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1. The "M" in ICM: using ATP to evaluate sanitation

Author:
Powitz, Robert W.
Source:
Foodsafety magazine 2009 v.15 no.2 pp. 28-31
ISSN:
1084-5984
Subject:
food industry; sanitation standard operating procedures; adenosine triphosphate; cleaning; cost effectiveness; food processing plants; food safety; food microbiology

2. 'In-line attack' conformational effect plays a modest role in an enzyme-catalyzed RNA cleavage: a free energy simulation study

Author:
Min, Donghong; Xue, Song; Li, Hong; Yang, Wei
Source:
Nucleic acids research 2007 v.35 no.12 pp. 4001-4006
ISSN:
0305-1048
Subject:
Gibbs free energy; RNA; adenosine triphosphate; aqueous solutions; catalytic activity; energy; enzyme substrates; enzymes; oligonucleotides
Abstract:
... Since the proposal of 'in-line attack' conformation as a possibly important intermediate in RNA cleavage, its structure has been captured in various protein and RNA enzymes; these structures strengthen the belief that this conformation plays an essential role in the catalysis of RNA cleavage. As generally discussed, this intermediate structure can be involved in energy barrier reduction in two pos ...
DOI:
10.1093/nar/gkm394
PubMed:
17553832
PubMed Central:
PMC1919504

3. (+)-Catechin potentiates the oxidative response of Acinetobacter baumannii to quinolone-based antibiotics

Author:
O.B. Ibitoye; T.O. Ajiboye
Source:
Microbial pathogenesis 2019 v.127 pp. 239-245
ISSN:
0882-4010
Subject:
Acinetobacter baumannii; adenosine diphosphate; adenosine triphosphate; antibacterial properties; antibiotics; catechol; ciprofloxacin; electron transport chain; glutathione; minimum inhibitory concentration; mutants; oxidative stress; pharmacology; reactive oxygen species; resorcinol; synergism
Abstract:
... (+)-Catechin is a versatile compound with its pharmacophore (catechol and resorcinol) responsible for prooxidant and antibacterial activities. In this study, we present that demonstrating the synergistic interaction between (+)-catechin and quinolone-based antibiotics, ciprofloxacin and gemifloxacin is related to reactive oxygen species generation. The minimum inhibitory concentration of (+)-catec ...
DOI:
10.1016/j.micpath.2018.12.012

4. (E)-2-Hexenal, as a Potential Natural Antifungal Compound, Inhibits Aspergillus flavus Spore Germination by Disrupting Mitochondrial Energy Metabolism

Author:
Weibin Ma; Luling Zhao; Wenhong Zhao; Yanli Xie
Source:
Journal of agricultural and food chemistry 2019 v.67 no.4 pp. 1138-1145
ISSN:
1520-5118
Subject:
Aspergillus flavus; acetyl coenzyme A; adenosine triphosphate; antifungal agents; apoptosis; crops; energy metabolism; enzymes; food production; human health; leaves; mechanism of action; median effective concentration; microbial contamination; minimum inhibitory concentration; mitochondria; phosphatidylserines; propidium; protein content; pyruvic acid; scanning electron microscopy; spore germination; spores; staining
Abstract:
... Fungal contamination imposes threats to agriculture and food production and human health. A method to safely and effectively restrict fungal contamination is still needed. Here, we report the effect and mode of action of (E)-2-hexenal, one of the green leaf volatiles (GLVs), on the spore germination of Aspergillus flavus, which can contaminate a variety of crops. The EC₅₀ value, minimum inhibitory ...
DOI:
10.1021/acs.jafc.8b06367

5. The (F1F0) ATP Synthase of Buchnera aphidicola (Endosymbiont of Aphids): Genetic Analysis of the Putative ATP Operon

Author:
Marta A. Clark; Paul Baumann
Source:
Current microbiology 1997 v.35 no.2 pp. 84-89
ISSN:
0343-8651
Subject:
Buchnera aphidicola; Escherichia coli; H+/K+-exchanging ATPase; H-transporting ATP synthase; Schizaphis graminum; adenosine triphosphate; chloroplasts; endosymbionts; genetic techniques and protocols; mitochondria; operon; prokaryotic cells; proteins
Abstract:
... Buchnera aphidicola is an intracellular, non-cultivable prokaryotic symbiont of the aphid Schizaphis graminum. A 6.8-kilobase fragment from B. aphidicola was cloned and sequenced and was found to contain genes encoding for proteins of the ATP synthase. The order of the genes, atpBEFHAGDC, is identical to that found in Escherichia coli and many other prokaryotes. This genetic organization is differ ...
DOI:
10.1007/s002849900217

6. (p-ClPhSe)2 stimulates carbohydrate metabolism and reverses the metabolic alterations induced by high fructose load in rats

Author:
Caroline B. Quines; Suzan G. Rosa; Pietro M. Chagas; Daniela Velasquez; Vinicius C. Prado; Cristina W. Nogueira
Source:
Food and chemical toxicology 2017 v.107 pp. 122-128
ISSN:
0278-6915
Subject:
adenosine triphosphate; blood glucose; carbohydrate metabolism; citrate (si)-synthase; dose response; fructose; glucose; glycogen; hexokinase; homeostasis; humans; isocitrate dehydrogenase; lipid metabolism; liver; rats; skeletal muscle; toxicology; triacylglycerols; tricarboxylic acid cycle
Abstract:
... The modern life leads to excess consumption of food rich in fructose; however, the long-term changes in carbohydrate and lipid metabolism could lead to metabolic dysfunction in humans. The present study evaluated the in vitro insulin-mimetic action of p-chloro-diphenyl diselenide (p-ClPhSe)2. The second aim of this study was to investigate if (p-ClPhSe)2 reverses metabolic dysfunction induced by f ...
DOI:
10.1016/j.fct.2017.06.038

7. (−) Epicatechin attenuates mitochondrial damage by enhancing mitochondrial multi-marker enzymes, adenosine triphosphate and lowering calcium in isoproterenol induced myocardial infarcted rats

Author:
P. Stanely Mainzen Prince
Source:
Food and chemical toxicology 2013 v.53 pp. 409-416
ISSN:
0278-6915
Subject:
adenosine triphosphate; alpha-ketoglutaric acid; body weight; calcium; cytochrome-c oxidase; electron transport chain; epicatechin; free radical scavengers; glutathione; glutathione peroxidase; glutathione-disulfide reductase; heart; hydroxyl radicals; in vitro studies; lipid peroxidation; malates; mitochondria; myocardial infarction; protective effect; rats; succinic acid; toxicology; tricarboxylic acid cycle
Abstract:
... Cardiac mitochondrial damage plays an important role in the pathology of myocardial infarction. The protective effects of (−) epicatechin on cardiac mitochondrial damage in isoproterenol induced myocardial infarction were evaluated in rats. Rats were pretreated with (−) epicatechin (20mg/kg body weight) daily for a period of 21days. After the pretreatment period, isoproterenol (100mg/kg body weigh ...
DOI:
10.1016/j.fct.2012.12.010

8. -Poly-l-Lysine Enhances Fruit Disease Resistance in Postharvest Longans (Dimocarpus longan Lour.) by Modulating Energy Status and ATPase Activity

Author:
Junzheng Sun; Hongbin Chen; Yihui Chen; Mengshi Lin; Yen-Con Hung; Yuji Jiang; Hetong Lin
Source:
Foods 2022 v.11 no.5 pp. -
ISSN:
2304-8158
Subject:
Diaporthe; Dimocarpus longan; adenosine diphosphate; adenosine monophosphate; adenosine triphosphate; adenosinetriphosphatase; ambient temperature; antibacterial properties; disease occurrence; disease resistance; energy; energy metabolism; food preservation; fruits; longans; mitochondria; vacuoles
Abstract:
... -poly-l-lysine (-PL) holds a strong antibacterial property and is widely used for food preservation. However, the application of -PL to enhance fruit disease resistance in postharvest longans (Dimocarpus longan Lour.) has not been explored. The objective of this study was to explore the impact of -PL treatment on disease occurrence and energy metabolism of longans infected with Phomopsis longanae ...
DOI:
10.3390/foods11050773

9. 036 Cryobiology of the Natural World: Linking the laboratory to the Field

Author:
Andrew Clarke
Source:
Cryobiology 2013 v.67 no.3 pp. 408
ISSN:
0011-2240
Subject:
adenosine triphosphate; animals; aquatic environment; boiling; cell membranes; chilling injury; cold; cryoprotectants; death; freezing; freezing point; ice; liquids; microorganisms; models; molecular weight; neurophysiology; plants (botany); seawater; solutes; temperature; vitrification; Alaska; Siberia
Abstract:
... Life has been found on Earth wherever water is liquid. Whilst considerable attention has been directed at microbes growing in boiling geysers and hydrothermal vents, much of the natural environment is seasonally or permanently cold. The coldest seawater is −1.96°C, but highly saline aquatic environments may reach −18°C. On land the interior of Siberia and Alaska may reach −60°C, a temperature whic ...
DOI:
10.1016/j.cryobiol.2013.09.042

10. 1 Assessing the energy status of porcine embryos by means of biodynamic imaging

Author:
I. Lorenzo; Z. Li; M. Torres; Z. Machaty; D. Nolte
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 125
ISSN:
1031-3613
Subject:
adenosine triphosphate; assisted reproductive technologies; bioluminescence assay; biomarkers; embryo (animal); energy; image analysis; microscopy; oocytes; oxidative phosphorylation; sodium azide; spectroscopy; swine; t-test; viability
Abstract:
... Assisted reproductive technologies are powerful tools for enhancing production in livestock or treating infertility in humans. Unfortunately, the success rate of the technologies is rather low. A major reason for the poor efficiency is the lack of methods to reliably assess the developmental potential of the embryos before transfer into recipients. Therefore, a noninvasive method to ensure the sel ...
DOI:
10.1071/RDv32n2Ab1

11. 1,2,3-Thiadiazolyl-Pheny 1-Ureas, New Inhibitors of Photosynthetic and Respiratory Energy Conservation

Author:
G. Hauska; A. Trebst; C. Kötter; H. Schulz
Source:
Zeitschrift für Naturforschung 2014 v.30 no.7-8 pp. 505-510
ISSN:
1865-7125
Subject:
adenosine triphosphate; aromatic compounds; cell respiration; chloroplasts; energy conservation; energy transfer; mechanism of action; mitochondria; photosynthesis; urea
Abstract:
... Substituted 1,2,3-thiadiazolyl-phenyl-ureas were found to be inhibitors of energy conservation in respiration and photosynthesis. The most effective dichlorophenylderivative uncouples ATP formation in isolated chloroplasts or mitochondria, at a concentration of about 2 and 9 μм respectively. At a certain concentration range the compounds also appear to be energy transfer inhibitors, similar to the ...
DOI:
10.1515/znc-1975-7-814

12. 1,25-Dihydroxyvitamin D3/vitamin D receptor suppresses brown adipocyte differentiation and mitochondrial respiration

Author:
Carolyn J. Ricciardi; Jiyoung Bae; Debora Esposito; Slavko Komarnytsky; Pan Hu; Jiangang Chen; Ling Zhao
Source:
European journal of nutrition 2015 v.54 no.6 pp. 1001-1012
ISSN:
1436-6207
Subject:
adenosine triphosphate; adipocytes; adults; animal models; brown adipose tissue; energy expenditure; enzymes; gene overexpression; heat production; humans; knockout mutants; messenger RNA; mice; obesity; oxygen consumption; transcriptional activation; vitamin D
Abstract:
... PURPOSE: The vitamin D system plays a role in metabolism regulation. 1,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃) suppressed 3T3-L1 white adipocyte differentiation. Vitamin D receptor (VDR) knockout mice showed increased energy expenditure, whereas mice with adipose-specific VDR over-expression showed decreased energy expenditure. Brown adipose tissue (BAT), now known to be present in adult humans, func ...
DOI:
10.1007/s00394-014-0778-9

13. 1,3-Diethylurea-enhanced Mg-ATPase activity of skeletal muscle myosin with a converse effect on the sliding motility

Author:
Tetsuichi Wazawa; Shin-ichiro Yasui; Nobuyuki Morimoto; Makoto Suzuki
Source:
BBA - Proteins and Proteomics 2013 v.1834 pp. 2620-2629
ISSN:
1570-9639
Subject:
actin; adenosine triphosphate; adenosinetriphosphatase; chickens; heavy meromyosin; microfilaments; skeletal muscle; thiourea; urea
Abstract:
... We investigate the effects of urea and its derivatives on the ATPase activity and on the in vitro motility of chicken skeletal muscle actomyosin. Mg-ATPase rate of myosin subfragment-1 (S1) is increased by 4-fold by 0.3M 1,3-diethylurea (DEU), but it is unaffected by urea, thiourea, and 1,3-dimethylurea at ≤1M concentration. Thus, we further examine the effects of DEU in comparison to those of ure ...
DOI:
10.1016/j.bbapap.2013.08.003

14. 1,5-Diaminonaphthalene Hydrochloride Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Small Molecules in Tissues Following Focal Cerebral Ischemia

Author:
Liu Huihui; Chen Rui; Wang Jiyun; Chen Suming; Xiong Caiqiao; Wang Jianing; Hou Jian; He Qing; Zhang Ning; Nie Zongxiu; Mao Lanqun
Source:
Analytical chemistry 2014 v.86 no.20 pp. 10114-10121
ISSN:
1520-6882
Subject:
absorption; adenosine triphosphate; amino acids; antioxidants; biomedical research; brain; brain damage; carboxylic acids; desorption; image analysis; ionization; ischemia; kidneys; liver; mass spectrometry; metabolism; metabolites; metal ions; mice; phospholipids; rats; salt tolerance; tissues; tricarboxylic acid cycle
Abstract:
... A sensitive analytical technique for visualizing small endogenous molecules simultaneously is of great significance for clearly elucidating metabolic mechanisms during pathological progression. In the present study, 1,5-naphthalenediamine (1,5-DAN) hydrochloride was prepared for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) of small molecules in liver, brain, ...
DOI:
10.1021/ac5034566

15. 1,8-Naphthalimide modified [12]aneN3 compounds as selective and sensitive probes for Cu2+ ions and ATP in aqueous solution and living cells

Author:
Yong-Guang Gao; Quan Tang; You-Di Shi; Ying Zhang; Zhong-Lin Lu
Source:
Talanta 2016 v.152 pp. 438-446
ISSN:
0039-9140
Subject:
adenosine triphosphate; aqueous solutions; copper; cytotoxicity; fluorescence; fluorescent dyes; human cell lines; image analysis; metal ions; titration; water solubility
Abstract:
... A new fluorescent probe 1 featuring one 1,8-naphthalimide and two [12]aneN3 units was synthesized. In the presence of Cu²⁺ ions, the fluorescence emission of 1 was quenched by a factor of 127-fold and no interference by other metal ions was observed under physiological conditions. By means of titration and a Job's plot it was established that 1 forms a complex with Cu²⁺ ions in a 1:2 ratio. The fl ...
DOI:
10.1016/j.talanta.2016.02.040

16. 1,8-Naphthyridine-based boron complexes: visible colorimetric probes for highly selective sensing of phosphoric ion

Author:
Gao Fen Wu; Qiu Lin Xu; Lin E. Guo; Tie Nan Zang; Rui Tan; Si Ting Tao; Jian Fei Ji; Rui Ting Hao; Jun Feng Zhang; Ying Zhou
Source:
Tetrahedron letters 2015 v.56 no.35 pp. 5034-5038
ISSN:
0040-4039
Subject:
Caenorhabditis elegans; absorbance; adenosine diphosphate; adenosine monophosphate; adenosine triphosphate; anions; boron; chemical reactions; chemical species; color; colorimetry; dimethyl sulfoxide; fluorescence; guanosine monophosphate; guanosine triphosphate; human cell lines; image analysis; moieties; pH; phosphates; uridine diphosphate; uridine monophosphate; uridine triphosphate
Abstract:
... Two 1,8-naphthyridine-based N,O-chelated boron complexes (1 and 2) were designed as novel phosphate ion (Pi) probes, which are based on a methoxy oxalyl group reaction site. These two probes showed high selectivity for Pi detection without interference from ATP, PPi, or other anions. Dramatic color changes from colorless to light yellow were observed by addition of Pi to solutions of 1 and 2 in DM ...
DOI:
10.1016/j.tetlet.2015.07.025

17. 1-Chloro-2,4-dinitrobenzene-elicited increase in vacuolar glutathione-S-conjugate transport activity

Author:
Li, Z.S.; Zhen, R.G.; Rea, P.A.
Source:
Plant physiology 1995 v.109 no.1 pp. 177-185
ISSN:
0032-0889
Subject:
Vigna radiata; vacuoles; glutathione; glutathione transferase; hypocotyls; seedlings; seedling growth; zymogens; enzyme activity; active transport; magnesium; adenosine triphosphate; kinetics; hydrolases; plasma membrane; translocation (plant physiology)
Abstract:
... Unlike most other characterized organic solute transport in plants, uptake of the model compound S-(2,4-dinitrophenyl)glutathione (DNP-GS) and related glutathione-S-conjugates by vacuolar membranes is directly energized by MgATP. Here we show that exogenous application of the DNP-GS precursor 1-chloro-2,4-dinitrobenzene (CDNB) to seedlings of Vigna radiata (mung bean) increases the capacity of vac ...

18. 1-Methyl-4-phenylpyridinium affects fast axonal transport by activation of caspase and protein kinase C

Author:
Morfini, G.; Pigino, G.; Opalach, K.; Serulle, Y.; Moreira, J.E.; Sugimori, M.; Llinás, R.R.; Brady, S.T.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2007 v.104 no.7 pp. 2442-2447
ISSN:
0027-8424
Subject:
Parkinson disease; adenosine triphosphate; animal models; axonal transport; caspases; humans; metabolites; neurons; neuropathology; neurotoxicity; neurotoxins; pathogenesis; protein kinase C; squid
Abstract:
... Parkinson's disease (PD), a late-onset condition characterized by dysfunction and loss of dopaminergic neurons in the substantia nigra, has both sporadic and neurotoxic forms. Neurotoxins such as 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and its metabolite 1-methyl-4-phenylpyridinium (MPP+) induce PD symptoms and recapitulate major pathological hallmarks of PD in human and animal models. Both s ...
DOI:
10.1073/pnas.0611231104
PubMed:
17287338
PubMed Central:
PMC1892945

19. 1-Methylcyclopropene (1-MCP) slows ripening of kiwifruit and affects energy status, membrane fatty acid contents and cell membrane integrity

Author:
Hua Huang; Lifang Guo; Ling Wang; Hui Wang; Sanmei Ma; Yueming Jiang; Hongxia Qu
Source:
Postharvest biology and technology 2019 v.156 pp. 110941
ISSN:
0925-5214
Subject:
1-methylcyclopropene; adenosine diphosphate; adenosine monophosphate; adenosine triphosphate; cell membranes; energy content; fatty acid composition; firmness; fruits; kiwifruit; lipid peroxidation; membrane permeability; palmitic acid; ripening; unsaturated fatty acids
Abstract:
... Changes of energy status and membrane fatty acids during ripening of kiwifruit without as with 1-methylcyclopropene (1-MCP) treatment have been investigated. Fruit were treated without (as control) or with 1 μL L⁻¹ 1-MCP for 24 h and then kept at 22 ± 2 °C for 16 d. In comparison to control, 1-MCP treatment inhibited the increase of membrane permeability, lipid peroxidation, and palmitic acid, the ...
DOI:
10.1016/j.postharvbio.2019.110941

20. 1-Methylcyclopropene alleviates chilling injury by regulating energy metabolism and fatty acid content in ‘Nanguo’ pears

Author:
Shunchang Cheng; Baodong Wei; Qian Zhou; Dehong Tan; Shujuan Ji
Source:
Postharvest biology and technology 2015 v.109 pp. 130-136
ISSN:
0925-5214
Subject:
1-methylcyclopropene; adenosine triphosphate; chilling injury; cold storage; cytochrome-c oxidase; energy; energy content; energy metabolism; enzyme activity; fatty acid composition; fatty acid metabolism; malondialdehyde; pears; saturated fatty acids; shelf life
Abstract:
... This study investigated the effects of 1-methylcyclopropene (1-MCP) treatment on chilling injury (CI), energy metabolism, and membrane fatty acid content in harvested ‘Nanguo’ pears during the shelf life after cold storage at 0°C. 1-MCP treated fruit showed slower CI injury development, lower ion leakage and malondialdehyde accumulation, increased adenosine triphosphate content and energy charge d ...
DOI:
10.1016/j.postharvbio.2015.05.012

21. 1-Methylcyclopropene alleviates peel browning of ‘Nanguo’ pears by regulating energy, antioxidant and lipid metabolisms after long term refrigeration

Author:
Dongbing Tao; Junwei Wang; Lei Zhang; Yangao Jiang; Mei Lv
Source:
Scientia horticulturae 2019 v.247 pp. 254-263
ISSN:
0304-4238
Subject:
Pyrus ussuriensis; shelf life; electrolytes; H+/K+-exchanging ATPase; vacuoles; NADH dehydrogenase; glutathione; malondialdehyde; hydrogen peroxide; ambient temperature; pears; gene expression; superoxide anion; adenosinetriphosphatase; energy; H-transporting ATP synthase; linoleate 13S-lipoxygenase; adenosine triphosphate; firmness; refrigeration; antioxidant activity; lipid metabolism; cold; 1-methylcyclopropene; fruits; ethylene production; cell respiration; glutathione peroxidase; sulfides; phospholipase D; cold storage
Abstract:
... Ripening of ‘Nanguo’ pears (Pyrus ussuriensis Maxim.) can be slowed down by cold storage, but the peel of fruit is prone to browning when they are returned to room temperature. In this study, effects of 1-methylcyclopropene (1-MCP) treatment on peel browning (PB) of ‘Nanguo’ pears, as well as on energy, antioxidant and lipid metabolisms, were investigated during shelf life after cold storage. 1-MC ...
DOI:
10.1016/j.scienta.2018.12.025

22. 108 Single-Cell RNA Sequencing Reveals Blastomere Heterogeneity and Early Lineage Specification Events in Bovine Embryos During Major Embryonic Genome Activation

Author:
I. Lavagi; S. Krebs; K. Simmet; V. Zakhartchenko; E. Wolf; H. Blum
Source:
Reproduction, fertility, and development 2018 v.30 no.1 pp. 193-194
ISSN:
1031-3613
Subject:
RNA; RNA splicing; adenosine triphosphate; barcoding; blastocyst; blastomeres; cDNA libraries; cattle; cell division; embryogenesis; gene ontology; genes; microvilli; mitochondria; polymerase chain reaction; quality control; sequence analysis; transcriptome; translation (genetics); zona pellucida
Abstract:
... During early embryonic stages, gene products generated by the embryo acquire control over embryonic development. At the 8- to 16-cell stage, major embryonic genome activation (EGA) occurs in bovine embryos. Morphological observations, such as size of blastomeres and distribution of microvilli, suggest heterogeneity of individual cells already at this developmental stage. To study this heterogeneit ...
DOI:
10.1071/RDv30n1Ab107

23. 123 Development of cryopreservation protocol for zebrafish ovarian fragments using controlled slow cooling

Author:
S. Anil; D.M. Rawson; T. Zhang
Source:
Cryobiology 2013 v.67 no.3 pp. 433
ISSN:
0011-2240
Subject:
Danio rerio; acetates; adenosine triphosphate; ambient temperature; aquaculture; cooling; cryopreservation; cryoprotectants; eggs; fish; fisheries management; fluorescein; freezing; germplasm; humans; methanol; nitrogen; oocytes; ovarian follicles; potassium chloride; propidium; sowing; spermatozoa; staining; storage time; straw; survival rate; viability
Abstract:
... Cryopreservation of gametes is an important method of conservation of germplasm and has wide range of applications in aquaculture and fisheries management. Cryopreservation is the most efficient method for long term storage of genetic materials. Though fish sperm cryopreservation has been a success, developing successful protocols for eggs and embryo cryopreservation still remain elusive. Several ...
DOI:
10.1016/j.cryobiol.2013.09.129

24. 13C metabolic flux analysis at a genome-scale

Author:
Saratram Gopalakrishnan; Costas D. Maranas
Source:
Metabolic engineering 2015 v.32 pp. 12-22
ISSN:
1096-7176
Subject:
NAD (coenzyme); NADP (coenzyme); adenosine triphosphate; arginine; biomass; carbon; confidence interval; culture media; fermentation; gluconeogenesis; glucose; glycolysis; metabolic flux analysis; metabolites; models; prediction; topology
Abstract:
... Metabolic models used in 13C metabolic flux analysis generally include a limited number of reactions primarily from central metabolism. They typically omit degradation pathways, complete cofactor balances, and atom transition contributions for reactions outside central metabolism. This study addresses the impact on prediction fidelity of scaling-up mapping models to a genome-scale. The core mappin ...
DOI:
10.1016/j.ymben.2015.08.006

25. 13C-metabolic flux analysis in S-adenosyl-l-methionine production by Saccharomyces cerevisiae

Author:
Kenshi Hayakawa; Shuichi Kajihata; Fumio Matsuda; Hiroshi Shimizu
Source:
Journal of bioscience and bioengineering 2015 v.120 no.5 pp. 532-538
ISSN:
1389-1723
Subject:
S-adenosylmethionine; Saccharomyces cerevisiae; adenosine triphosphate; drugs; oxidative phosphorylation; tricarboxylic acid cycle; yeasts
Abstract:
... S-Adenosyl-l-methionine (SAM) is a major biological methyl group donor, and is used as a nutritional supplement and prescription drug. Yeast is used for the industrial production of SAM owing to its high intracellular SAM concentrations. To determine the regulation mechanisms responsible for such high SAM production, ¹³C-metabolic flux analysis (¹³C-MFA) was conducted to compare the flux distribut ...
DOI:
10.1016/j.jbiosc.2015.03.010

26. 13C-metabolic flux analysis of lipid accumulation in the oleaginous fungus Mucor circinelloides

Author:
Lina Zhao; Huaiyuan Zhang; Liping Wang; Haiqin Chen; Yong Q. Chen; Wei Chen; Yuanda Song
Source:
Bioresource technology 2015 v.197 pp. 23-29
ISSN:
0960-8524
Subject:
Mucor circinelloides; NADP (coenzyme); acetyl coenzyme A; adenosine triphosphate; amino acids; citrates; gamma-linolenic acid; gas chromatography-mass spectrometry; glyoxylate cycle; malic enzyme; oleaginous fungi; pentose phosphate cycle; tricarboxylic acid cycle
Abstract:
... The oleaginous fungus Mucor circinelloides is of industrial interest because it can produce high levels of polyunsaturated fatty acid γ-linolenic acid. M. circinelloides CBS 277.49 is able to accumulate less than 15% of cell dry weight as lipids, while M. circinelloides WJ11 can accumulate lipid up to 36%. In order to better understand the mechanisms behind the differential lipid accumulation in t ...
DOI:
10.1016/j.biortech.2015.08.035

27. 14,15-EET promotes mitochondrial biogenesis and protects cortical neurons against oxygen/glucose deprivation-induced apoptosis

Author:
Lai Wang; Man Chen; Lin Yuan; Yuting Xiang; Ruimao Zheng; Shigong Zhu
Source:
Biochemical and biophysical research communications 2014 v.450 pp. 604-609
ISSN:
0006-291X
Subject:
adenosine triphosphate; apoptosis; arachidonic acid; binding proteins; biogenesis; brain; cell viability; cortex; gene expression regulation; glucose; ischemia; membrane potential; messenger RNA; metabolites; mitochondria; mitochondrial DNA; mitochondrial membrane; neurons; neuroprotective effect; nuclear genome; oxygen; small interfering RNA; transcription factors
Abstract:
... 14,15-Epoxyeicosatrienoic acid (14,15-EET), a metabolite of arachidonic acid, is enriched in the brain cortex and exerts protective effect against neuronal apoptosis induced by ischemia/reperfusion. Although apoptosis has been well recognized to be closely associated with mitochondrial biogenesis and function, it is still unclear whether the neuroprotective effect of 14,15-EET is mediated by promo ...
DOI:
10.1016/j.bbrc.2014.06.022

28. 14-3-3 protein is a regulator of the mitochondrial and chloroplast ATP synthase

Author:
Bunney, T.D.; Walraven, H.S. van.; Boer, A.H. de.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2001 v.98 no.7 pp. 4249-4254
ISSN:
0027-8424
Subject:
mitochondria; chloroplasts; pyrophosphatases; binding proteins; enzyme activity; adenosine triphosphate; hydrolysis; oxygen; oxygen consumption; phosphorylation
Abstract:
... Mitochondrial and chloroplast ATP synthases are key enzymes in plant metabolism, providing cells with ATP, the universal energy currency. ATP synthases use a transmembrane electrochemical proton gradient to drive synthesis of ATP. The enzyme complexes function as miniature rotary engines, ensuring energy coupling with very high efficiency. Although our understanding of the structure and functionin ...
DOI:
10.1073/pnas.061437498
PubMed:
11274449
PubMed Central:
PMC31211

29. 143 Stimulated glycolysis is able to maintain ATP levels and motility of bull spermatozoa submitted to mitochondrial depolarisation

Author:
J. Losano; J. Padín; I. Méndez-López; D. Angrimani; N. Montez; A. García; V. Barnabe; M. Nichi
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 198
ISSN:
1031-3613
Subject:
adenosine triphosphate; albumins; analysis of variance; bulls; cyanides; glucose; glycolysis; lactic acid; luminescence; mitochondria; oxidative phosphorylation; pyruvic acid; spermatozoa
Abstract:
... Studies have reported the importance of mitochondria in sperm metabolism. However, for some species, glycolysis appears to be as essential as oxidative phosphorylation in sperm physiology. On the other hand, these mechanisms have not been fully elucidated for bovine spermatozoa. Therefore, the aim of this study was to evaluate the role of mitochondria and glycolysis in ATP synthesis and sperm kine ...
DOI:
10.1071/RDv32n2Ab143

30. 161 Dichloroacetate influences the mitochondrial activity of bovine oocytes impairing meiotic progression

Author:
N. Pagano; K. Annes; C. De Canditiis; J. Ispada; B. Gasparrini; M. Milazzotto
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 205-206
ISSN:
1031-3613
Subject:
NAD (coenzyme); acetyl coenzyme A; adenosine triphosphate; analysis of variance; cattle; culture media; embryo (animal); embryo culture; embryogenesis; energy; flavin-adenine dinucleotide; hydrides; meiosis; membrane potential; metabolism; mice; mitochondria; mitochondrial membrane; oocytes; oxidation; oxidative stress; pyruvate dehydrogenase (acetyl-transferring) kinase; pyruvate dehydrogenase (lipoamide); pyruvic acid; reactive oxygen species; staining; tricarboxylic acid cycle
Abstract:
... Pyruvate is a key energy substrate for the oocyte during maturation and acquisition of developmental competence. Mitochondrial activity is also essential for oocyte competence. Dichloroacetate (DCA) is an inhibitor of pyruvate dehydrogenase kinase that indirectly stimulates pyruvate dehydrogenase (PDH), increasing pyruvate oxidation. PDH converts pyruvate into acetyl coenzyme A (acetyl-CoA) and th ...
DOI:
10.1071/RDv31n1Ab161

31. 18O-Exchange by Hydrolyzing Enzymes: An ab initio Calculation

Author:
Paul Rösch; Hans Robert Kalbitzer; Roger S. Goody
Source:
Zeitschrift für Naturforschung 2014 v.36 no.7-8 pp. 534-538
ISSN:
1865-7125
Subject:
adenosine diphosphate; adenosine triphosphate; enzymes; myosin
Abstract:
... Enzymes which hydrolyse ATP cause an exchange of 180 of labelled Pi in the presence of ADP. A theory for the evaluation of rate constants from an observation of the time dependence of the concentration of the various Pi species is presented. Application to the 180 exchange catalysed by myosin SI as observed by 31P-NMR shows excellent agreement with values of the rate constants determined earlier. ...
DOI:
10.1515/znc-1981-7-804

32. 1H NMR metabolomics of earthworm responses to polychlorinated biphenyl (PCB) exposure in soil

Author:
Melissa L. Whitfield Åslund; André J. Simpson; Myrna J. Simpson
Source:
Ecotoxicology 2011 v.20 no.4 pp. 836-846
ISSN:
0963-9292
Subject:
Eisenia fetida; adenosine triphosphate; correlation; earthworms; metabolism; metabolites; metabolomics; nuclear magnetic resonance spectroscopy; polychlorinated biphenyls; soil; soil toxicity
Abstract:
... 1H NMR-based metabolomics was used to examine the metabolic profile of D2O-buffer extracted tissues of Eisenia fetida earthworms exposed for 2 days to an artificial soil spiked with sub-lethal concentrations of polychlorinated biphenyls (PCBs) (0, 0.5, 1, 5, 10, or 25 mg/kg Aroclor 1254). Univariate statistical analysis of the identified metabolites revealed a significant increase in ATP concentra ...
DOI:
10.1007/s10646-011-0638-9

33. 1H NMR-based metabolomic analysis of cuttlefish, Sepia pharaonis (Ehrenberg, 1831) exposed to hypoxia stresses and post-anoxia recovery

Author:
Maowang Jiang; Hua Yang; Ruibing Peng; Qingxi Han; Xiamin Jiang
Source:
Science of the total environment 2020 v.726 pp. 138317
ISSN:
0048-9697
Subject:
Sepia pharaonis; adenosine; adenosine triphosphate; alanine; amino acid metabolism; arginine; betaine; biochemical pathways; brain; choline; coastal ecosystems; energy; estuaries; gamma-aminobutyric acid; gills; glutamine; glycogen; hypoxia; lactic acid; metabolites; metabolomics; neurotransmitters; nuclear magnetic resonance spectroscopy; taurine
Abstract:
... Oxygen deficiency (hypoxia and anoxia) is an emerging concern in estuarine and coastal ecosystems worldwide. Previous studies on Mollusca Cephalopoda have focused on the effects of hypoxia stress on physiological performance and survival, but there are few reports on the molecular mechanism, and the application of metabolomics in cephalopods remains unknown. In this study, a ¹H nuclear magnetic re ...
DOI:
10.1016/j.scitotenv.2020.138317

34. 1H NMR-based metabolomics study on the physiological variations during the rat pregnancy process

Author:
Guiping Shen; Zhishui Li; Yan Zhang; Huifeng Wu; Jianghua Feng
Source:
Molecular and Cellular Endocrinology 2016 v.423 pp. 40-50
ISSN:
0303-7207
Subject:
ABC transporters; adenosine triphosphate; biochemical pathways; carbohydrate metabolism; digestion; fetal development; growth and development; metabolites; metabolomics; physiological response; physiological state; pregnancy; rats
Abstract:
... In this study, NMR-based metabolomics in combination with multivariate pattern recognition technologies were employed to evaluate the physiological variations in the Wistar rats' plasma that are induced by pregnancy on the gestational days (GDs) 11, 14, 17 and 20. Untargeted metabolomics analysis revealed some possible mechanism of physiological effects for healthy pregnancies and showed a metabol ...
DOI:
10.1016/j.mce.2016.01.003

35. 1H and 31P nuclear magnetic resonance and kinetic studies of the active site structure of chloroplast CF1 ATP synthase

Author:
Devlin, C.C.; Grisham, C.M.
Source:
Biochemistry 1990 v.29 no.26 pp. 6192-6203
ISSN:
0006-2960
Subject:
Spinacia; chloroplasts; adenosine triphosphate; ligases; manganese; kinetics; spectral analysis; nuclear magnetic resonance spectroscopy; binding sites
DOI:
10.1021/bi00478a012
PubMed:
2145032

36. 1H-NMR analysis of the human urinary metabolome in response to an 18-month multi-component exercise program and calcium–vitamin-D3 supplementation in older men

Author:
John R. Sheedy; Paul R. Gooley; Amsha Nahid; Dedreia L. Tull; Malcolm J. McConville; Sonja Kukuljan; Caryl A. Nowson; Robin M. Daly; Peter R. Ebeling
Source:
Applied Physiology, Nutrition, and Metabolism 2014 v.39 no.11 pp. 1294-1304
ISSN:
1715-5320
Subject:
adenosine triphosphate; biomarkers; bone density; bone metabolism; calcium; choline; creatinine; exercise; fortified foods; humans; hypoxanthine; lean body mass; men; metabolism; metabolites; metabolome; metabolomics; milk consumption; monitoring; muscle strength; muscles; nuclear magnetic resonance spectroscopy; patients; randomized clinical trials; urinalysis; urine
Abstract:
... The musculoskeletal benefits of calcium and vitamin-D₃ supplementation and exercise have been extensively studied, but the effect on metabolism remains contentious. Urine samples were analyzed by ¹H-NMR spectroscopy from participants recruited for an 18-month, randomized controlled trial of a multi-component exercise program and calcium and vitamin-D₃ fortified milk consumption. It was shown previ ...
DOI:
10.1139/apnm-2014-0060

37. 1H-NMR-based metabolomic studies of bisphenol A in zebrafish (Danio rerio)

Author:
Changshin Yoon; Dahye Yoon; Junghee Cho; Siwon Kim; Heonho Lee; Hyeonsoo Choi; Suhkmann Kim
Source:
Journal of environmental science and health 2017 v.52 no.4 pp. 282-289
ISSN:
1532-4109
Subject:
Danio rerio; adenosine triphosphate; bisphenol A; discriminant analysis; glutamine; lactic acid; metabolites; metabolome; metabolomics; nuclear magnetic resonance spectroscopy; oxidative stress; succinic acid
Abstract:
... Proton nuclear magnetic resonance (¹H-NMR) spectroscopy was used to study the response of zebrafish (Danio rerio) to increasing concentrations of bisphenol A (4,4′-(propane-2,2-diyl)diphenol, BPA). Orthogonal partial least squares discriminant analysis (OPLS-DA) was applied to detect aberrant metabolomic profiles after 72 h of BPA exposure at all levels tested (0.01, 0.1, and 1.0 mg/L). The OPLS-D ...
DOI:
10.1080/03601234.2016.1273009

38. 1‐Methylcyclopropene treatment controls ethanol accumulation associated with regulation of mitochondrial energy metabolism in kiwifruit (Actinidia deliciosa) cv. “Bruno” during storage at room temperature

Author:
Maratab Ali; Muhammad Ammar Raza; Shenge Li; Chen Huan; Xiaolin Zheng
Source:
Journal of food biochemistry 2020 v.44 no.7 pp. e13273
ISSN:
0145-8884
Subject:
1-methylcyclopropene; Actinidia deliciosa; adenosine triphosphate; alanine transaminase; ambient temperature; aromatic compounds; citric acid; climacteric fruits; cytochrome-c oxidase; decarboxylation; energy; ethanol; kiwifruit; metabolites; mitochondria; off flavors; oxidative phosphorylation; pyruvate dehydrogenase (lipoamide); pyruvic acid; tricarboxylic acid cycle; China
Abstract:
... In this paper, the effects of 1‐methylcyclopropene (1‐MCP) treatment on the ethanol accumulation and mitochondrial energy metabolism in kiwifruit (Actinidia deliciosa) cv. “Bruno” were investigated during storage at room temperature (24 ± 1°C). The results showed that 1‐MCP treatment significantly reduced the ethanol accumulation, maintained higher levels of adenosine triphosphate (ATP) content an ...
DOI:
10.1111/jfbc.13273

39. A 2 bp deletion in the mitochondrial ATP 6 gene responsible for the NARP (neuropathy, ataxia, and retinitis pigmentosa) syndrome

Author:
Patrick Mordel; Stéphane Schaeffer; Quentin Dupas; Marie-Alice Laville; Marion Gérard; Françoise Chapon; S. Allouche
Source:
Biochemical and biophysical research communications 2017 v.494 no.1-2 pp. 133-137
ISSN:
0006-291X
Subject:
adenosine triphosphate; adenosinetriphosphatase; ataxia (disorder); blood; deafness; epilepsy; fibroblasts; fluorescent dyes; gene deletion; genes; high-throughput nucleotide sequencing; membrane potential; mitochondria; mitochondrial DNA; mitochondrial membrane; muscles; oligomycin; patients; peripheral nervous system diseases
Abstract:
... Mitochondrial (mt) DNA-associated NARP (neurogenic muscle weakness, ataxia, and retinitis pigmentosa) syndrome is due to mutation in the MT-ATP6 gene.We report the case of a 18-year-old man who presented with deafness, a myoclonic epilepsy, muscle weakness since the age of 10 and further developed a retinitis pigmentosa and ataxia. The whole mtDNA analysis by next-generation sequencing revealed th ...
DOI:
10.1016/j.bbrc.2017.10.066

40. 2,3-Butandione 2-monoxime inhibits skeletal myosin II by accelerating ATP cleavage

Author:
Hideyuki Komatsu; Yuji Koseki; Takeshi Kanno; Shunsuke Aoki; Takao Kodama
Source:
Biochemical and biophysical research communications 2017 pp. -
ISSN:
0006-291X
Subject:
acetylcholinesterase; adenosine; adenosine triphosphate; adenosinetriphosphatase; mechanism of action; myosin; nuclear magnetic resonance spectroscopy; phosphorus; potassium; stable isotopes
Abstract:
... 2,3-Butandione 2-monoxime (BDM) is a widely used myosin inhibitor with an unclear mode of action. In this report, we investigated the mechanism of BDM oxime group nucleophilic reactivity on the phosphoester bond of ATP. BDM increased the ATPase activity of skeletal myosin subfragment 1 (S1) under conditions in which ATP cleavage is the rate-limiting step (K+, EDTA-ATPase activity of native S1 and ...
DOI:
10.1016/j.bbrc.2017.06.130

41. 2,3-Dihydroxy-quinoxaline induces ATPase activity of Herpes Simplex Virus thymidine kinase

Author:
Alexey A. Zeifman; Fedor N. Novikov; Victor S. Stroylov; Oleg V. Stroganov; Ghermes G. Chilov; Alexander Y. Skoblov; Anatoly I. Miroshnikov; Yuri S. Skoblov
Source:
FEBS letters 2014 v.588 no.3 pp. 509-511
ISSN:
0014-5793
Subject:
adenosine triphosphate; adenosinetriphosphatase; catalytic activity; herpes simplex; hydrolysis; phosphorylation; screening; thymidine kinase; viruses
Abstract:
... 2,3-Dihydroxy-quinoxaline, a small molecule that promotes ATPase catalytic activity of Herpes Simplex Virus thymidine kinase (HSV-TK), was identified by virtual screening. This compound competitively inhibited HSV-TK catalyzed phosphorylation of acyclovir with Ki=250μM (95% CI: 106–405μM) and dose-dependently increased the rate of the ATP hydrolysis with KM=112μM (95% CI: 28–195μM). The kinetic sc ...
DOI:
10.1016/j.febslet.2013.12.017

42. 2,4-Dinitrophenol reduces the reactivity of Lys553 in the lower 50-kDa region of myosin subfragment 1

Author:
Theresa R. Bomfim; Luciana E.S.F. Machado; Luis Mauricio T.R. Lima; Martha M. Sorenson; Verônica P. Salerno
Source:
Archives of biochemistry and biophysics 2011 v.505 no.1 pp. 105-111
ISSN:
0003-9861
Subject:
2,4-dinitrophenol; actin; adenosine triphosphate; binding sites; enzyme activity; fluorescence; hexanoic acid; hydrophobicity; magnesium; molecular conformation; myosin
Abstract:
... 2,4-Dinitrophenol (DNP) increases the affinity of myosin for actin and accelerates its Mg²⁺ATPase activity, suggesting that it acts on a region of the myosin head that transmits conformational changes to actin- and ATP-binding sites. The binding site/s for DNP are unknown; however similar hydrophobic compounds bind to the 50-kDa subfragment of the myosin head, near the actin-binding interface. In ...
DOI:
10.1016/j.abb.2010.09.022

43. 2-Amino-3-carboxy-1,4-naphthoquinone affects the end-product profile of bifidobacteria through the mediated oxidation of NAD(P)H

Author:
Yamazaki, S.; Kaneko, T.; Taketomo, N.; Kano, K.; Ikeda, T.
Source:
Applied microbiology and biotechnology 2002 v.59 no.1 pp. 72-78
ISSN:
0175-7598
Subject:
Bifidobacterium breve; Bifidobacterium longum; NAD (coenzyme); NAD(P)H dehydrogenase (quinone); NADP (coenzyme); acetates; adenosine triphosphate; fructose 6-phosphate; glucose; iron; lactate dehydrogenase; metabolism; metabolites; oxidation; pentose phosphate cycle; pyruvic acid
Abstract:
... Glucose metabolism of bifidobacteria in the presence of 2-amino-3-carboxy-1,4-naphthoquinone (ACNQ), a specific growth stimulator for bifidobacteria, and ferricyanide (Fe(CN)6(3-) as an extracellular electron acceptor was examined using resting cells of Bifidobacterium longum and Bifidobacterium breve. NAD(P)H in the cells is oxidized by ACNQ with the aid of diaphorase activity, and reduced ACNQ d ...
DOI:
10.1007/s00253-002-0982-z
PubMed:
12073135

44. 2-Amino-nonyl-6-methoxyl-tetralin muriate activity against Candida albicans augments endogenous reactive oxygen species production - a microarray analysis study

Author:
Liang, Rong Mei; Yong, Xiao Lan; Jiang, Yun Ping; Tan, Yong Hong; Dai, Bao Di; Wang, Shi Hua; Hu, Ting Ting; Chen, Xi; Li, Nan; Dong, Zhao Hui; Huang, Xiao Chun; Chen, Jun; Cao, Yong Bing; Jiang, Yuan Ying
Source:
FEBS journal 2011 v.278 no.7 pp. 1075-1085
ISSN:
1742-464X
Subject:
mechanism of action; Candida albicans; mitochondrial membrane; oligonucleotides; energy; drugs; microarray technology; reactive oxygen species; drug resistance; electron transfer; adenosine triphosphate; aerobiosis; genes; ascorbic acid; patients; gene expression regulation; membrane potential; antifungal properties
Abstract:
... Candida infections have become an increasingly significant problem, mainly because of the widespread nature of Candida and drug resistance. There is an urgent need to develop new classes of drugs for the treatment of opportunistic Candida infections, especially in medically complex patients. Previous studies have confirmed that 2-amino-nonyl-6-methoxyl-tetralin muriate (10b) possesses powerful ant ...
DOI:
10.1111/j.1742-4658.2011.08021.x

45. 2-Deoxyglucose Induces Cell Cycle Arrest and Apoptosisin Colorectal Cancer Cells Independent of Its Glycolysis Inhibition

Author:
Pratik Muley; Alex Olinger; Hemachand Tummala
Source:
Nutrition and cancer 2015 v.67 no.3 pp. 514-522
ISSN:
1532-7914
Subject:
2-deoxyglucose; adenosine triphosphate; antineoplastic agents; apoptosis; cell cycle checkpoints; colorectal neoplasms; drug therapy; glycolysis; hexokinase; interphase; neoplasm cells
Abstract:
... 2-Deoxyglucose (2DG) is an anticancer drug with excellent safety profile. Because of its higher dose requirements, its potential is yet to translate into a monotherapy. However, recently, 2DG has been tested as an adjunct in established chemotherapeutic regimens. 2DG enhanced the potency of several chemotherapeutic agents but not all. The rationale selection of known chemotherapeutic agents to use ...
DOI:
10.1080/01635581.2015.1002626

46. 2-Deoxyglucose induces the expression of thioredoxin interacting protein (TXNIP) by increasing O-GlcNAcylation – Implications for targeting the Warburg effect in cancer cells

Author:
Shin Yee Hong; Thilo Hagen
Source:
Biochemical and biophysical research communications 2015 v.465 pp. 838-844
ISSN:
0006-291X
Subject:
2-deoxyglucose; adenosine triphosphate; endoplasmic reticulum; glucose; glycolysis; hexokinase; neoplasm cells; neoplasms; oxygen; therapeutics; tumor suppressor proteins
Abstract:
... The high proliferation rate of cancer cells and the microenvironment in the tumor tissue require the reprogramming of tumor cell metabolism. The major mechanism of metabolic reprogramming in cancer cells is the Warburg effect, defined as the preferential utilization of glucose via glycolysis even in the presence of oxygen. Targeting the Warburg effect is considered as a promising therapeutic strat ...
DOI:
10.1016/j.bbrc.2015.08.097

47. 2-Deoxyglucose treatment complements the cisplatin- or BH3-only mimetic-induced suppression of neuroblastoma cell growth

Author:
Jiin-Haur Chuang; Ming-Huei Chou; Ming-Hong Tai; Tsu-Kung Lin; Chia-Wei Liou; Tingya Chen; Wen-Ming Hsu; Pei-Wen Wang
Source:
international journal of biochemistry & cell biology 2013 v.45 no.5 pp. 944-951
ISSN:
1357-2725
Subject:
2-deoxyglucose; adenosine triphosphate; apoptosis; cell growth; cell proliferation; cisplatin; glycolysis; hexokinase; hypoxia-inducible factor 1
Abstract:
... Neuroblastoma (NB) is characterized by pleomorphic molecular characteristics, which may influence cellular metabolism as well as the efficacy of glycolytic inhibitors in suppressing NB cell growth. We studied the metabolic profile of four NB cell lines without or with MYCN amplification and found no unanimous metabolic characteristics. The two NB cell lines with MYCN amplification exhibited a sign ...
DOI:
10.1016/j.biocel.2013.01.019

48. 2-Hydroxyisocaproyl-CoA dehydratase and its activator from Clostridium difficile

Author:
Kim, Jihoe; Darley, Daniel; Buckel, Wolfgang
Source:
FEBS journal 2005 v.272 no.2 pp. 550-561
ISSN:
1742-464X
Subject:
Clostridium difficile; Escherichia coli; adenosine triphosphate; fermentation; genes; hydrolysis; leucine; magnesium chloride; metal ions; molybdenum; nitrogenase; sulfur; zinc
Abstract:
... The hadBC and hadI genes from Clostridium difficile were functionally expressed in Escherichia coli and shown to encode the novel 2-hydroxyisocaproyl-CoA dehydratase HadBC and its activator HadI. The activated enzyme catalyses the dehydration of (R)-2-hydroxyisocaproyl-CoA to isocaprenoyl-CoA in the pathway of leucine fermentation. The extremely oxygen-sensitive homodimeric activator as well as th ...
DOI:
10.1111/j.1742-4658.2004.04498.x

49. The 2-arachidonoylglycerol effect on myosin light chain phosphorylation in human platelets

Author:
Maria Grazia Signorello; Enrica Giacobbe; Mario Passalacqua; Giuliana Leoncini
Source:
Biochimie 2013 v.95 pp. 1620-1628
ISSN:
0300-9084
Subject:
actin; adenosine triphosphate; calcium; humans; myosin light chains; phosphorylation; polymerization; protein kinase C; secretion
Abstract:
... In human platelets the endocannabinoid 2-arachidonoylglycerol (2-AG) stimulates some important pathways leading to thromboxane B2 formation, calcium intracellular elevation, ATP secretion and actin polymerisation. The aim of the present study was to examine the 2-AG effect on myosin light chain (MLC) phosphorylation and to investigate the mechanisms involved. We demonstrated that 2-AG induced a ra ...
DOI:
10.1016/j.biochi.2013.05.003

50. 20(S)-ginsenoside Rg3, a neuroprotective agent, inhibits mitochondrial permeability transition pores in rat brain

Author:
Tian, Jingwei; Zhang, Shumin; Li, Guisheng; Liu, Zhifeng; Xu, Benming
Source:
Phytotherapy research 2009 v.23 no.4 pp. 486-491
ISSN:
0951-418X
Subject:
Oriental traditional medicine; Panax ginseng; adenosine triphosphate; antioxidant activity; biochemical mechanisms; brain; energy metabolism; free radical scavengers; herbal medicines; mitochondria; neuroprotective effect; rats; reactive oxygen species; triterpenoids
Abstract:
... Ginseng, the root of Panax ginseng C.A. Meyer (Araliaceae), is a well-known traditional Chinese herbal medicine. Ginsenosides, which are triterpene derivatives that contain sugar moieties, are the main active ingredients of ginseng. 20(S)-Ginsenoside Rg3, a triterpene glycoside which chemically belongs to the protopanaxadiol ginsenoside group, is effective in attenuating brain infarction after cer ...
DOI:
10.1002/ptr.2653
PubMed:
19003949