An official website of the United States government
Here’s how you know
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
... For a rapid, specific and sensitive identification of cows', ewes' and goats' milk in mono-species Sicilian dairy products, species-specific duplex-PCR protocol was applied. DNA samples from blood and experimental cheeses of Sicilian autochthonous breeds were extracted to amplify the 12S rRNA (and part of 16S rRNA in case of Ovis aries) mitochondrial species-specific gene fragment. The use of spec ...
... Illegal adulteration of melamine in animal feed and food has been widely studied. However, the risk of using substitute non-protein nitrogen substances still exists. In this study, we developed the ¹³C NMR method for the detection of non-protein nitrogen substance adulteration in animal feed. Three compounds, i.e., urea, melamine, and biuret, were used for method development. We found that the che ...
Yvonne Gunning; Marianne Defernez; Andrew D. Watson; Niles Beadman; Ian J. Colquhoun; Gwénaëlle Le Gall; Mark Philo; Hollie Garwood; David Williamson; Aaron P. Davis; E. Kate Kemsley
adulterated products; lipophilicity; liquid chromatography; mass spectrometry; monitoring; nuclear magnetic resonance spectroscopy
Abstract:
... High-field and low-field proton NMR spectroscopy were used to analyse lipophilic extracts from ground roast coffees. Using a sample preparation method that produced concentrated extracts, a small marker peak at 3.16 ppm was observed in 30 Arabica coffees of assured origin. This signal has previously been believed absent from Arabicas, and has been used as a marker for detecting adulteration with r ...
adulterated products; chemometrics; discriminant analysis; least squares; models; nuclear magnetic resonance spectroscopy; prediction; principal component analysis; rapid methods; vegetable oil
Abstract:
... Proton nuclear magnetic resonance (1H NMR) and chemometrics were employed to detect the adulteration of camellia oil (CAO) with 3 different cheap vegetable oils. With the intensity of 15 selected 1H NMR signals as input variables, principal component analysis (PCA) showed good group clustering results for pure and nonpure CAO, but unsatisfied identification accuracy for the adulterated oil types, ...
adulterated products; apples; byproducts; chemical species; food chemistry; gas chromatography-mass spectrometry; isotope dilution technique; methanol; neurotoxins; nuclear magnetic resonance spectroscopy; pectins; stable isotopes; wines
Abstract:
... Methanol, a hepato- and neurotoxic compound, is present in apple-based beverages as a by-product of the enzymatic degradation of pectin. Stable isotope dilution assay (SIDA) employing gas chromatography-mass spectrometry (GC–MS) is the state-of-the-art method for methanol determination in beverages. Despite higher initial investment costs, quantitative ¹H NMR spectroscopy (qNMR) is a simpler and f ...
... In this work, we tested a NMR‐based MVA method to assess the authenticity of EVOOs cultivar (from specific origin) declaration by using a total of over a 100 samples coming from different selected areas of Southern Italian Regions (Apulia and Calabria). We built a reference model, by performing unsupervised PCA and supervised PLS‐DA, OPLS‐DA analyses on the ¹HNMR spectra of a 40 monovarietal EVOOs ...
... Paprika powder is a widely consumed high-priced product and therefore particularly prone to fraudulent practices. Nuclear magnetic resonance spectroscopy combined with one-class classification was applied for non-targeted detection of paprika adulteration and further chemometric tools were tested for diagnostic purposes. The ¹H NMR spectra of 186 commercial paprika powders and 216 spiked samples w ...
... A 1H NMR analytical protocol for the detection of refined hazelnut oils in admixtures with refined olive oils is reported according to ISO format. The main purpose of this research activity is to suggest a novel analytical methodology easily usable by operators with a basic knowledge of NMR spectroscopy. The protocol, developed on 92 oil samples of different origins within the European MEDEO proje ...
... Chinese Yams (Dioscorea polystachya Turczaninow) are mainly used as functional food and traditional Chinese medicine in China. Adulteration is a major problem in the food and herbal industry and has affected the commercial use of natural products. Here, a ¹H NMR-based metabolomics approach was used for the identification of the distinguishing metabolites between geo-authentic Chinese yam (D. polys ...
adulterated products; high performance liquid chromatography; honey; markets; nuclear magnetic resonance spectroscopy; rice; syrups
Abstract:
... The determination of honey authenticity is of importance to ensure its quality and safety. There is an urgent need of effective methods to detect adulterated honey. A simple, rapid, and effective HPLC–DAD method was developed to detect honey adulteration by rice syrup, using a characteristic compound from rice syrup, which is presently difficult to detect by current analytical methods. The charact ...
... Determination of changes in 2-furoylmethyl amino acids and hydroxymethylfurfural during the storage of four honey samples at 25 and 35 °C during 12 months was achieved to assess the potential use of both parameters, singly or in combination, as quality indicators. 2-Furoylmethyl amino acids increased during storage at both temperatures, whereas hydroxymethylfurfural only presented slight variation ...
... The level of 2-glyceryl monopalmitate (2-GMP) measures adulteration in olive oil with esterified oils. However, there are genuine refined olive and refined olive-pomace oils that do not meet the specifications of the standard set by the International Olive Council. For this reason, there is a need to investigate the factors that affect 2-GMP levels in olive and in olive pomace oil. The results sho ...
adulterated products; air; death; manufacturing; melamine; milk; nitrogen; protein content; renal failure; traceability; urinary bladder neoplasms; China
Abstract:
... Milk is one of the most important food products for children’s growth and overall health. Melamine (2,4,6-triazine-1,3,5-triamino) is an organic compound used in the manufacture of pesticides, plastics, sanitizers, and disinfectants. Melamine when added to milk increases the overall amount of nitrogen in the milk thus fooling common tests for protein content. Melamine is also extremely harmful whe ...
adulterated products; aeration; cream; electrical resistance; milk; milk industry; milk tanks; powders; process monitoring; processing stages; temperature; tomography; total solids
Abstract:
... The novel application of Electrical Resistance Tomography (ERT) in monitoring milk solutions could have potential applications in various stages of the milk processing industry for monitoring and control of crucial parameters. In this study, Electrical Resistance Tomography has been applied for the first time in 3-dimensional visualization of milk holding tanks for analysis of overall homogeneity ...
Fourier transform infrared spectroscopy; adulterated products; chemical bonding; chemometrics; cooking fats and oils; detection limit; hazelnut oil; nuclear magnetic resonance spectroscopy; olive oil; screening; spectrometers; vibration
Abstract:
... We report the first results from a new 60MHz ¹H nuclear magnetic resonance (NMR) bench-top spectrometer, Pulsar, in a study simulating the adulteration of olive oil with hazelnut oil. There were qualitative differences between spectra from the two oil types. A single internal ratio of two isolated groups of peaks could detect hazelnut oil in olive oil at the level of ∼13%w/w, whereas a whole-spect ...
... In the century of research chronicled between 1917 and 2017, dairy goats have gone from simply serving as surrogates to cows to serving as transgenic carriers of human enzymes. Goat milk has been an important part of human nutrition for millennia, in part because of the greater similarity of goat milk to human milk, softer curd formation, higher proportion of small milk fat globules, and different ...
... Processed meat is a target in meat adulteration for economic gain. This study demonstrates a molecular and bioinformatics diagnostic pipeline, utilizing the mitochondrial 16S ribosomal RNA (rRNA) gene, to determine processed meat product mislabeling through Next-Generation Sequencing. Nine pure meat samples were collected and artificially mixed at different ratios to verify the specificity and sen ...
adulterated products; chemometrics; food fraud; public health; spectroscopy; whisky
Abstract:
... Food fraud and adulteration is a major concern in terms of economic and public health. Multivariate methods combined with spectroscopic techniques have shown promise as a novel analytical strategy for addressing issues related to food fraud that cannot be solved by the analysis of one variable, particularly in complex matrices such distilled beverages. This review describes and discusses different ...
... Campylobacter is one of the leading causes of foodborne travelers’ diarrhea worldwide. Although a large number cases of campylobacteriosis go undiagnosed or unreported, it is considered as the second most common foodborne illness in the USA affecting over 1.3 million individuals every year. Of various Campylobacter species, C. jejuni, C. coli, and C. lari have been accounted for causing more than ...
Camellia sinensis; Chinese people; adulterated products; alkaloids; amino acids; antimicrobial properties; antioxidant activity; body weight; chemical composition; chemical constituents of plants; disease control; green tea; minerals; monitoring; pharmaceutical industry; polyphenols; proteins; quality control; shrubs; trees; vitamins
Abstract:
... Tea, Camellia sinensis, which belongs to the family Theaceae, is a shrub or evergreen tree up to 16 m in height. Green tea is very popular because of its marked health benefits comprising its anticancer, anti-oxidant, and antimicrobial activities, as well as its effectiveness in reducing body weight. Additionally, it was recognized by Chinese people as an effective traditional drink required for t ...
... Honey is a highly consumed natural product, not only for its taste and nutritional value, but also for its health benefits. Owing to characteristics that are essentially or exclusively related to the specific region or particular local environment and flora, honey can be classified as a premium product generally perceived as a high‐quality and valued product because of its desirable flavor and tas ...
Bharathi Avula; Iffat Parveen; Jianping Zhao; Mei Wang; Natascha Techen; Yan-Hong Wang; Mohammad Riaz; Ji-Yeong Bae; Anter A. Shami; Amar G. Chittiboyina; Ikhlas A. Khan; Joshua S. Sharp
adulterated products; case studies; polyacrylamide gel electrophoresis; protein content; proteomics; quality control; supply chain; whey protein
Abstract:
... The presence of bio-macromolecules as major ingredients is a primary factor in marketing many biologically derived macromolecular supplements. Workflows for analyzing these supplements for quality assurance, adulteration, and other supply-chain difficulties must include a qualitative assessment of small-molecule and macromolecular components; however, no such integrated protocol has been reported ...
adulterated products; beta-lactoglobulin; cheeses; cluster analysis; control methods; densitometry; desorption; detection limit; digestion; freeze drying; gels; ionization; mass spectrometry; milk; monitoring; polyacrylamide gel electrophoresis; sodium dodecyl sulfate; trypsin; whey
Abstract:
... This study introduces a qualitative method to inspect the compositional authenticity of white nonripened cheeses like Minas Frescal, a typical Brazilian cheese, especially when irregular replacement of milk by whey is suspected. A sodium dodecyl sulfate gel electrophoresis (SDSâPAGE) method, followed by image densitometry, was validated. Cheeses were freeze-dried to electrophoresis, and β-lacto ...
... DNA-based assays offer precision in ascertaining the species/cultivar origin of agro-food products. Yet, obtaining DNA of sufficient quality and quantity is the main challenge while performing DNA-based food authentication analyses. The aim of the present work was to standardize a cost-efficient, easy-to-apply, yet effective plant oil DNA isolation protocol that allows reliable downstream PCR-base ...
... Considering the recent cases of meat adulteration and fraud, efficient and accurate analytical methods are needed for the specific identification of meat species as a key importance to maintain consumers' trust and to comply with various local labelling legislations. As a reference approach, the application of PCR to meat speciation is always limited to few species, whereas there is a strong need ...
... This work is concerned with an analytical method for detecting acai adulteration based on digital image (DI) assisted by mean one-class classification (OCC) chemometric approaches, namely data-driven soft independent modeling of class analogy (DD-SIMCA) and one-class partial least squares (OC-PLS). In this study, two adulterants were considered, wheat flour and cassava. Digital images were acquire ...
... The presence of pork content in food products can be a serious issue over an adulteration of food, particularly for consumers who pay attention to halal certification or other religious beliefs concerning the consumption of pork. Due to this fact, finding a method for the detection of pork content in food products that is sensitive, fast, and accurate is of interest. In this study, an electrochemi ...
pork; Trichinella; drug therapy; adulterated products; raw meat; Muslims; disease severity; trichinosis; humans; morbidity; disease outbreaks; zoonoses; disease prevalence; food contamination; food pathogens; foodborne illness; beef; signs and symptoms (animals and humans); Turkey (country)
Abstract:
... An outbreak of trichinellosis occurred in Izmir, Turkey, between January and March 2004. The outbreak was caused by the consumption of raw meat balls made of beef deceptively mixed with pork infected with Trichinella britovi. A total of 1098 people who had consumed this food either in 14 restaurants or from the street vendors located in three different neighbourhoods, consulted six different healt ...
Laura Di Francesco; Francesco Di Girolamo; Maurizio Mennini; Andrea Masotti; Guglielmo Salvatori; Giuliano Rigon; Fabrizio Signore; Emanuela Pietrantoni; Margherita Scapaticci; Isabella Lante; Bianca Maria Goffredo; Oscar Mazzina; Ahmed Ibrahim Elbousify; Paola Roncada; Andrea Dotta; Alessandro Fiocchi; Lorenza Putignani
adulterated products; ass milk; breast milk; buffalo milk; camel milk; colostrum; ewe milk; goat milk; infant formulas; lactation; matrix-assisted laser desorption-ionization mass spectrometry; milk; milk composition; multivariate analysis; peptides; pipelines; protein content; proteomics
Abstract:
... Human milk composition is dynamic, and substitute formulae are intended to mimic its protein content. The purpose of this study was to investigate the potentiality of matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS), followed by multivariate data analyses as a tool to analyze the peptide profiles of mammalian, human, and formula milks. Breast milk samples ...
... Adulteration of meat in processed food is a sensitive issue since certain meat species are prohibited in some religions such as Islam and Judaism. Some meat types are also potential carrier of some deadly diseases such as severe acute respiratory syndrome, anthrax, and hepatitis. Furthermore, unconscious consumption of certain meat might lead to an allergic reaction. Feline meat is not only taboo ...
DNA; adulterated products; common markets; developed countries; electrophoresis; fraud; genetic polymorphism; grape must; grapes; markets; mass spectrometry; microsatellite repeats; polymerase chain reaction; traceability; winemaking; wines; Italy
Abstract:
... The process of wine traceability/authentication is a key target for Italy, the world’s first largest wine producer (around 4.6 million tons in 2010 according to FAOSTAT). Several European countries developed appellation systems, with their own unique labels and seals, trying to fight label fraud that misrepresented the true origins of the wine. Advancements on this topic were provided by Council R ...
Camellia sinensis; DNA; DNA barcoding; adulterated products; cultivars; dust; genome; green tea; libraries; risk; vegetation; India
Abstract:
... Exploration on DNA-based investigations is frequently obscured by being constrained to work in suboptimal conditions that incorporate minimal sampling regimes, crucial tissue type, and amount. As nucleic acids are the source to explain different inquiries in DNA-involved investigations there is a need for a trendy strategy to affirm entire genome from complicated heterogeneous vegetation. In this ...
... With an increased consumption of seafood products, food fraud with fish resources has been continuously reported. In particular, codfish has been exploited worldwide as a processed product in fresh, frozen, smoked, canned, or ready-to-eat dish forms. However, it is challenging to identify processed fish products after processing because of their similar morphological characteristics. Substitution ...
Tylopilus; models; least squares; near-infrared spectroscopy; adulterated products; chemometrics; mushrooms; information processing; Boletus edulis; Italy; China
Abstract:
... Boletus edulis and allied species (BEAS), known as “porcini mushrooms”, represent almost the totality of wild mushrooms placed on the Italian market, both fresh and dehydrated. Furthermore, considerable amounts of these dried fungi are imported from China. The presence of Tylopilus spp. and other extraneous species (i.e., species edible but not belonging to BEAS) within dried porcini mushrooms – m ...
adulterated products; chromatography; color; humans; quality control
Abstract:
... To establish a new method of testing and evaluating the quality of refined montan wax (RMW), digital color and GC fingerprint technology were introduced and applied. CIE Lab color mode was used to digitize the exterior colors of RMW, and the score obtained through a fitting function was also used to reflect its quality. It is shown that they were in complete accord with the human visual perception ...
... A liquid chromatographic method based on determining of the amount of lactic acid was developed to detect milk adulteration with neutralizers. The developed method can be applied to milk with pH values within the regular range of 6.5–6.7 that is suspected of being neutralised. Determination of lactic acid was carried out in milk acidified with lactic acid and neutralised with sodium hydroxide to s ...
sugars; food analysis; high fructose corn syrup; adulterated products; carbohydrate composition; mass spectrometry; gas chromatography; honey
Abstract:
... Honey adulterations can be carried out by addition of inexpensive sugar syrups, such as high fructose corn syrup (HFCS) and inverted syrup (IS). Carbohydrate composition of 20 honey samples (16 nectar and 4 honeydew honeys) and 6 syrups has been studied by GC and GC-MS in order to detect differences between both sample groups. The presence of difructose anhydrides (DFAs) in these syrups is describ ...
... Many oils sold in China and India are a blend of various oils to improve performance, stability, and nutritional characteristics, which are required in their respective markets. Quantitative analysis of the proportions of constitutive components is fundamental to the conformity and adulteration checking of edible blended oil products. A multi linear regression model with constrained linear least s ...
... High-performance electrochemical enzymatic biosensor based on flowerlike α-Fe₂O₃ nanostructures was successfully developed for the detection of potential food adulterant, formaldehyde (formalin). The biosensor was found to be highly sensitive (744.15 μA mg⁻¹ Lcm⁻²) with linear range of detection (0.01–0.3 mg/L) and showed high shelf-life (9 weeks) and precision (0.73% RSD) with reasonably good rep ...
... A simple and novel method to quantify adulterations of extra virgin olive oil (EVOO) with refined olive oil (ROO) and refined olive−pomace oil (ROPO) is described here. This method consists of calculating chaotic parameters (Lyapunov exponent, autocorrelation coefficients, and two fractal dimensions, CPs) from UV−vis scans of adulterated EVOO samples. These parameters have been successfully linear ...
... Potassium bromate is used in the baking industry as a bread improver and is known to improve dough kneading characteristics and bread volume. However, over the years, its carcinogenic nature has been established and KBrO₃ is considered a hazardous adulterant in food systems. This study aimed to develop and validate a novel powder x-ray diffraction (PXRD) method for the detection of KBrO₃ adulterat ...
... Hypericum perforatum L. (Hypericaceae) is a medicinal plant of considerable interest for the therapeutic potentialities of its biologically active compounds. Due to the presence of hybrids and frequent adulterants from other species of Hypericum, the identification of the drug obtained of this species is difficult. Therefore, a quantitative morphological analysis of the leaf epidermises of H. hirc ...
... In this work, a reasonable substitute with laborious and expensive liquid chromatography for quick melamine detection in milk and dairy products is outlined. To accomplish this aim, first‐order multivariate calibration methods including partial least squares (PLS) and net analyte preprocessing‐partial least squares (NAP/PLS) are adopted for melamine quantification in complex food extracts down to ...
... Quantification of colorimetric assays with smartphones is being increasingly reported. However, a complete characterization of the performance of existing color spaces and single-color channels for optimum color/intensity change quantification is absent. Moreover, it has not been ascertained if it is necessary to utilize existing color spaces to reach optimal assay quantification. In this study, a ...
... Concerns over the potential adulteration of commercially produced foods give rise to a requirement for a simple and fast analytical method capable of quantifying potential adulterants. This work demonstrates a simple HPLC method tailored to detect major organic acids and furans within ingredients in commercial food products, for example, pomegranate molasses, balsamic vinegar, and apple cider vine ...
... A rapid, accurate, and efficient method for determining the sterol, uvaol, and erythrodiol concentrations was developed to meet International Olive Council (IOC) certification criteria for extra virgin olive oil (EVOO). The unsaponifiable fraction of the sample (0.2 g) was separated with a diatomaceous earth column, and the sterol and triterpenic dialcohols were isolated with a novel base-activate ...
Fourier transform infrared spectroscopy; adulterants; adulterated products; black pepper; bulking agents; chemical composition; image analysis; microscopy; principal component analysis; probability
Abstract:
... Infrared spectroscopy is often used as a simple, fast, and green method to screen for economically motivated adulteration in spices. However, conventional microscopy remains the reference method. In this research, the combination of microscopy and Fourier-transform infrared spectroscopy in mapping mode, namely, micro-FTIR imaging, along with Principle Component Analysis were used to develop a non- ...
adulterated products; corn; models; nuclear magnetic resonance spectroscopy; olive oil; olives; soybeans; sunflower seed; support vector machines; vegetable oil
Abstract:
... Due to the quality differentiation and commercial concerns, rapid authentication and addressing the adulterants in olive oil is of great importance. The feasibility of identifying pure olive oil as well as classifying the binary blends of olive oils according to the adulterants in olive oils using low-field NMR spectroscopy and support vector machine (SVM) have been investigated. Based on the char ...
adulterated products; biofuels; derivatization; detection limit; gasoline; government agencies; internal combustion engines; ionization; mass spectrometry; methanol; organochlorine compounds; solvents
Abstract:
... Methanol is an organic compound commonly used as a solvent in the synthesis of fuel for internal combustion engines. However, the adulteration of fuels (biofuels and gasoline) with methanol is commonly practiced throughout world, and regulations to control the content of methanol in these fuels have been imposed. Although methanol in fuels can be analyzed by numerous analytical techniques, the tra ...
... Rice importance resides in its high consumption mainly in Asia and Africa and less in the EU. Several cultivars, both GM and non-GM, have established themselves in various regions depending mainly on the climatic and soil conditions. A high number of analytical, enzymic, and genomic analyses (instrumental) in conjunction with sensory analysis were applied (not always very successfully) towards det ...
... Authenticity and traceability have been two of the most important issues in the food chain. Authenticity in particular, is closely related with both food quality and safety issues. Vegetables stand for a category of foods heavily affected by adulteration either in terms of geographic origin (national or international level) or production methods (organic or conventional production, fertilizers, pe ...
... Extra virgin olive oil (EVOO) has elevated commercial value due to its health appeal, desirable characteristics and quantitatively limited production, and thus it has become an object of intentional adulteration. As EVOOs on the market might consist of a blend of olive varieties or sometimes even of a mixture of oils from different botanical species, an array of DNA-fingerprinting methods have bee ...
... Extra virgin olive oil has been one of the most common targets for economically motivated food adulteration. This research employed the hand‐held FTIR spectrometer to develop the simple and portable screening methods for extra virgin olive oils adulterated with other cheaper vegetable oils. With the help of the ATR probe and chemometrics tools, oil samples can be measured directly without any pret ...
... Multiplex PCR methods have been frequently used for authentication of meat product adulteration. Through screening of new species-specific primers designed based on the mitochondrial DNA sequences, a septuple PCR method is ultimately developed and optimized to simultaneously detect seven species including turkey (110 bp), goose (194 bp), pig (254 bp), sheep (329 bp), beef (473 bp), chicken (612 bp ...
adulterated products; cameras; color; colorimetry; milk; mobile telephones; monitoring; nanofibers; pH; prototypes; support vector machines
Abstract:
... Development of an economical miniaturized platform for monitoring inherent biophysical properties of milk is imperative for tamper-proof milk adulteration detection. Towards this, herein, we demonstrate synthesis and evaluation of a paper-based scalable pH sensor derived from electrospun halochromic nanofibers. The sensor manifests into three unique color-signatures corresponding to pure (6.6 ≤ pH ...
... Cereal crops play an important role in preventing chronic diseases and regulating human functions due to their rich phytochemicals. However, an increasing number of cases of cereal‐crop adulteration are occurring, affecting the nutrition of food and threatening food safety. Therefore, this study used hyperspectral imaging (HSI) and a back‐propagation neural network (BPNN) to establish the variety ...
... In the present work, a barcode-DNA analysis method is described for the detection of plant oil adulteration in milk and dairy products. The method relies on the fact that plant DNA should not be present in readily detectable amounts in a dairy product unless it contains undeclared plant material. Thus, a universal plant barcode is chosen as the target to be amplified from dairy samples. Accordingl ...
... Adulteration of antidiabetic herbal medicines with hypoglycemic drugs has been reported as a recurrent practice throughout the world. In this context, a new method for the determination of metformin, chlorpropamide, glibenclamide and gliclazide by capillary zone electrophoresis (CZE) with capacitively coupled contactless conductivity detection (C⁴D) is described in this paper. The proposed method ...
... Plant secondary metabolites are emerging as attractive alternatives in the development of therapeutics against infectious and chronic diseases. Due to the present pandemic, therapeutics showing toxicity against bacterial pathogens and viruses are gaining interest. Plant metabolites of terpenoid and phenylpropanoid categories have known antibacterial and antiviral properties. These metabolites have ...
... Solid-phase microextraction (SPME) using cross-linked polymeric ionic liquid (PIL)-based sorbent coatings was used to extract volatile aroma-related compounds from coffee samples. Several PIL-based coatings were screened alongside a commercial poly(acrylate) (PA) SPME coating. The best performing PIL-based SPME fiber, poly(1-vinyl-3-hexadecylimidazolium bis[(trifluoromethyl)sulfonylimide]) with 50 ...
... A biosensor based on a chiral assembly of polymer of gold nanoparticle (AuNP) trimers was developed for the detection and quantification of the major shellfish allergen tropomyosin (TROP). TROP and anti-TROP monoclonal antibodies (mAb) were immobilized on 20 nm and 30 nm 16-mercaptohexadecanoic acid (16-MHDA) functionalized AuNPs to assemble a trimer, which has a Circular dichroism (CD) signal. Th ...
... In the present study, a novel analytical platform is introduced, which enables both analysis and quality control of St John's wort extracts and tissue. The synergistic combination of separation techniques (including thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC)) with mass spectrometry (MS) and vibrational spectroscopy is demonstrated to get deeper insights into the ...
... Functional foods are products focused to human consumption with benefits to different physiologic aspects in order to prevent diverse metabolic problems in the body. Prebiotics recently have been started to be employed as functional foods, and fructans as inulin or agave inulin, known as agavin, represent a valuable source of them. Standard polysaccharides as starch, inulin, cellulose and agavin w ...
... The aim of this study was to develop the models based on the texture parameters of images of selected parts of pepper fruit for cultivar discrimination. This study was aimed to compare the usefulness of textures from cross‐sections (slices) and seeds of pepper for distinguishing the different cultivars using the discriminative classifiers. The textures from the cross‐sections and seeds were calcul ...
... Not-from-concentrate (NFC) juice has better nutrition, flavor and higher price than reconstituted juice. Accordingly, NFC juice is prone to adulteration and is an ongoing industry problem that has not yet been resolved. Undeclared addition of water and sugar are the main forms of NFC juice adulteration. This paper investigates the carbon and oxygen stable isotope ratios (δ¹³C and δ¹⁸O values) of t ...
adulterated products; ash content; briquettes; burning; charcoal; energy; fuels; issues and policy; monitoring; prices; renewable energy sources; urbanization; waste utilization; wastes; water content; Uganda
Abstract:
... As a result of the rising energy needs and environmental concerns, carbonized briquettes have been looked at as a possible substitute source of energy for charcoal in most of the developing regions. However their use and adoption in Uganda cannot be rated amidst continued increase in charcoal demand from the ever growing urbanization. This study therefore investigated burning performance and cost ...
... Lonicera japonica Thunb. is a well-known traditional herbal medicine in most East Asian countries. In China, the flower bud and stem of this plant are used for various clinical therapies, while the leaf is not officially recognized as an active part. Due to the similarities in their chemical constituents but great differences in their commercial values, the flower bud has been found to be adultera ...
Food and Agriculture Organization; World Health Organization; adulterated products; erythrosine; food additives; food law; foods; rural areas; India
Abstract:
... A study was carried out to find the type and level of food colours added to various foods in the city of Hyderabad and rural areas around Hyderabad. The majority of foods from both urban and rural areas contained permitted colours while some foods sold at kiosks contained non-permitted colours. The majority of the foods were found to contain colours exceeding the statutory limits. Of the eight syn ...
... This paper proposes a novel milk quality detection approach based on utilization of inherent biophysical properties as ‘markers’ for adulteration. Unlike the traditional adulterant-specific approaches, this method is generic and universal. It exploits the change in innate milk properties, such as electrical conductivity and pH, upon addition of adulterants as a transduction mechanism for detecting ...
... Vanilla, being the world's most popular flavoring materials, finds extensive applications in food, beverages, perfumery and pharmaceutical industry. With the high demand and limited supply of vanilla pods and the continuing increase in their cost, numerous efforts of blending and adulteration in natural vanilla extracts have been reported. Thus, to ensure the quality of vanilla extracts and vanill ...
... Fraud in the global food supply chain is becoming increasingly common due to the huge profits associated with this type of criminal activity. Food commodities and ingredients that are expensive and are part of complex supply chains are particularly vulnerable. Both herbs and spices fit these criteria perfectly and yet strategies to detect fraudulent adulteration are still far from robust. An FT-IR ...
European Union; Piper nigrum; adulterated products; black pepper; food industry; mass spectrometry; monoterpenoids; odors; small fruits; storage conditions; taste; volatile organic compounds
Abstract:
... Black pepper (BP) is globally the most widely used spice and is appreciated for its aroma and taste qualities. Its aroma is influenced by various factors. In the current study, we examined a reference set of 90 quality BP and 40 quality white pepper (WP) samples from the EU spice industry for their volatile organic compounds (VOC) by Proton Transfer Reaction - Mass Spectrometry, as well as 10 low- ...
adulterated products; capacitance; cluster analysis; electrodes; ethanol; ethanol fuels; principal component analysis; t-test; water content
Abstract:
... A new approach for the discrimination of the adulteration process of ethanol fuel with water is reported using a copper interdigitated electrode and chemometrical tools. The sensor was constructed using copper sheets with non-chemical modification of the electrode surface. The discrimination process was performed using capacitance values recorded at different frequencies (1000Hz to 0.1MHz) as the ...
DNA-directed DNA polymerase; adulterated products; agar gel electrophoresis; beef; chickens; color; cost effectiveness; cross reaction; equipment; food composition; mitochondrial DNA; nucleic acid hybridization; rapid methods; recombinase polymerase amplification; traceability; world markets
Abstract:
... Meat adulteration is becoming increasingly prominent in the global market. Therefore, a robust, sensitive, and affordable rapid detection strategy for identifying meat species is urgently needed. In this study, we developed a novel strategy using chicken-specific recombinase polymerase amplification (RPA) coupled with nucleic acid hybridization lateral flow strip (NAH-LFS) for the rapid detection ...
... Extra virgin olive oil (EVOO) is acknowledged as a liquid gold. It is cherished for its unique aroma, flavour and health benefits. Due to its high demand and high cost, fraudsters deliberately blend low-cost poor quality edible oil with EVOO to get economic benefits. The consumption of adulterated EVOO leads to health and safety issue of consumers. Thus, the rapid, precise, accurate and up-to-date ...
Food and Drug Administration; adulterated products; antibodies; dairy products; detection limit; enzyme-linked immunosorbent assay; fluorescence; foods; immunosensors; liquid chromatography; mass spectrometry; melamine
Abstract:
... We report a droplet-based microfluidic immunosensor for the rapid and accurate detection of melamine, an organic base that has been implicated in widescale adulteration of food products such as milk. Our melamine assay is based on the competitive reaction between native melamine and a melamine-fluorescein isothiocyanate (FITC) conjugate against an anti-hapten antibody. The adoption of fluorescence ...
... Orange juice is a kind of fruit juice with huge market share. The most common adulteration is the addition of mandarin juice and grapefruit juice. Therefore, it is necessary to develop a method that can simultaneously detect mandarin and grapefruit components in orange juice. In this study, the specific site of the trnL-trnF gene of mandarin and the specific site of the accD-psaI gene of grapefrui ...
... A duplex polymerase chain reaction (PCR) has been applied for the detection of both cows' and goats' milk in goats' milk cheeses using primers targeting the mitochondrial 12S rRNA genes. By means of a linear normalised calibration curve between the log of the ratio of the cows' band intensity and the sum of cows' and goats' intensities vs. the log of cows' milk percentage, it was possible to quant ...
... Supply chain has a principal role in supporting the development of milk processing industry. The supply chain of the dairy industry in Indonesia involves a number of actors and a variety of dairy handling that may lead into potential risks and failures. One of the risks affecting supply chain performance is the adulteration of raw materials. This condition can possibly result in the occurrence of ...
DNA; DNA barcoding; Oriental traditional medicine; Panax ginseng; Panax quinquefolius; adulterated products; genetic distance; genome; internal transcribed spacers; intraspecific variation; loci; medicinal plants; single nucleotide polymorphism
Abstract:
... Medicinal plants of the Panax genus belonging to Araliaceae family are well-known, rare plants used as tonics in traditional Chinese medicine and have been described in the Chinese Pharmacopoeia. Because of the high price and the huge human demand, these commercial products often contain adulterants. In this study, 377 sequences from four species were analyzed. Single nucleotide polymorphisms (SNP ...
... The substitution of high priced meat with low cost ones and the fraudulent labeling of meat products make the identification and traceability of meat species and their processed products in the food chain important. A polymerase chain reaction followed by a High Resolution Melting (HRM) analysis was developed for species specific detection of buffalo; it was applied in six commercial meat products ...
adulterants; adulterated products; combustion; cost effectiveness; ethanol; gasoline; oil and gas industry; paper; petroleum
Abstract:
... Adulteration of fuel is a global problem that needs to be curbed. Studies have proved that no technically easy solution has been developed and easily accessible to the Oil and gas industry to detect adulterated gasoline. The need therefore arises for adequate and substantial methods for easy detection of adulterated gasoline.•This study developed an effective and cost-efficient method to detect ad ...
adulterated products; carrageenan; gels; image analysis; least squares; magnetic resonance imaging; models; nuclear magnetic resonance spectroscopy; prediction; shrimp
Abstract:
... Gel injection for shrimp is a commonly adulterated way to obtain illegal profits by unscrupulous traders. In this study, a method based on low field nuclear magnetic resonance (LF-NMR) and magnetic resonance imaging was developed to investigate the gel-injected adulteration in a fast and non-destructive manner. Shrimp samples injected with carrageenan in the range of 0.2–1.2 mL at 0.2 mL increment ...
adulterated products; discriminant analysis; food industry; least squares; livestock breeding; microscopy; nonprotein nitrogen; raw materials; screening; soybean meal
Abstract:
... The quality and safety of soybean meal is a key matter for the livestock breeding and food industries, since it is one of the most important and widely used protein feed raw materials. As driven by commercial interests, new illegal adulterants which are unknown to consumers and regulators emerge constantly. In order to make up for the inadequacy of traditional detection methods, a novel non-target ...
... In this work, the polar metabolite pool of commercial caprine milk was studied by gas chromatography-mass spectrometry and multivariate statistical data analysis. Experimental data were compared with those of cow milk and the discriminant analysis correctly classified milk. By the same means, differences due to heat treatments (UHT or pasteurization) on milk samples were also investigated. Results ...
adulterated products; drug abuse; fenfluramine; graphene; obesity; solid phase extraction; tandem mass spectrometry; ultra-performance liquid chromatography
Abstract:
... Slimming supplements were popularly sold online driven by the increasement of obesity and the development of social networking platform. However, events of drug abuse in slimming supplements were also frequently reported. In this study, a graphene tip solid-phase extraction (Gtip SPE) and ultra-performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) method was established for deter ...
Sapium; acetates; adulterated products; benzaldehyde; chemometrics; databases; headspace analysis; honey; image analysis; least squares; phenylacetaldehyde; principal component analysis; spectroscopy; winter
Abstract:
... A simple and environmentally approach using untargeted imaging of volatile substances combined with chemometrics and markers response was proposed for discriminating different species of honey with headspace gas-chromatography-ion-mobility (HS-GC-IMS). The 3D HS-GC-IMS imaging and their response differences enabled the clear discrimination between winter honey and sapium honey. Principal component ...
... Due to their high market value, meat products are often targets for species substitution and adulteration. DNA-based methods are recognized as the most appropriate means to detect such fraudulent practices, however, these have not been extensively employed for the authentication of meat products available in South Africa. The aim of this study was to utilize a variety of molecular techniques to ev ...
... A novel closed-tube loop-mediated isothermal amplification (LAMP) was developed to detect goat milk adulterated with cow milk. The reaction results of the LAMP assay developed, identified using both visual observation and agarose gel electrophoresis, were completely consistent, indicating that visual observation was suitable and efficient to judge the final LAMP results. The specificity of LAMP as ...
... Milk processing industries and distributors have problems with adulteration of liquid milk by the addition of bovine cheese whey. Recently, the detection of fraudulent manipulation of milk with whey has focused on the identification of glycomacropeptide (GMP). Current non-immunological methods to detect GMP in dairy products are expensive and time-consuming or have low sensitivity. In this study, ...
... A highly specific single step polymerase chain reaction (PCR) is described for the detection of goat (Capra hircus) meat. Goat-specific primers (DAF-01 and DGR-04) were designed against a conserved region of mitochondrial d-loop gene that yielded a 294bp PCR product. Specificity of the primers was confirmed by testing them with DNA from other commonly used meat animal species, i.e., cattle, buffal ...
DNA primers; adulterated products; detection limit; gel electrophoresis; heat treatment; molecular weight; mutton; polymerase chain reaction; quality control; sheep
Abstract:
... A highly species-specific polymerase chain reaction (PCR) was developed for authentic identification of raw and heat treated sheep meat (mutton). Oligonucleotide primers were designed against a unique target region of sheep mitochondrial D-loop, that yielded four PCR products upon gel electrophoresis of which two amplicons of molecular size 329 and 404bp were found highly diagnostic. Chances of cr ...
DNA; adenosinetriphosphatase; adulterated products; cost effectiveness; cytochrome b; dogs; fast foods; food chain; fraud; genes; hot dogs; markets; meat; monkeys; polymerase chain reaction; public health; rats; risk; swine; wildlife
Abstract:
... Food forgery has posed considerable risk to public health, religious rituals, personal budget and wildlife. Pig, dog, cat, rat and monkey meat are restricted in most religions, but their sporadic adulteration are rampant. Market controllers need a low-cost but reliable technique to track and trace suspected species in the food chain. Considering the need, here we documented a lab-on-a-chip-based m ...
... A label free electrochemical immunosensor for sensitive detection of porcine serum albumin (PSA) is reported in this work. The immunosensor was constructed by first electrochemically reducing 4-carboxyphenyl diazonium salt, which had been electrochemically generated in situ, to a stable 4-carboxyphenyl layer on carbon nanofiber-modified screen printed electrode. Antibodies were covalently attached ...
... BACKGROUND: The authenticity of honey is of great importance for commercial and health reasons, because adulteration of bee honey with cheaper sweetening materials is sometimes carried out for financial gain. The objectives of this study were to evaluate clover honey quality according to physicochemical characteristics, to measure light transmission through honey samples using a visible laser tech ...
... Food fraud or food adulteration may be of forensic interest for instance in the case of suspected deliberate mislabeling. On account of its potential health benefits and nutritional qualities, geographical origin determination of olive oil might be of special interest. The use of a likelihood ratio (LR) model has certain advantages in contrast to typical chemometric methods because the LR model ta ...
... A new analytical method was developed to determine the presence of six (6) compounds with the potential to be used in economic adulteration to enhance the nitrogen content in milk products and bulk proteins. Residues were extracted from the matrix with 2% formic acid, after which acetonitrile (ACN) was added to induce precipitation of the proteins. Extracts were analyzed by liquid chromatography u ...