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- Dähne, Lars, et al. Show all 6 Authors
- Applied microbiology and biotechnology 2017 v.101 no.2 pp. 599-607
- Escherichia coli; Pseudozyma antarctica; acetates; ambient temperature; biomass; buffers; carboxylic ester hydrolases; cell culture; coatings; culture media; drying; filters; half life; models; pH; polycyclic aromatic hydrocarbons; polymers
- ... The objective of this study was to use for the first time depth filters, which are usually intended for clarification of cell culture broth, as a direct immobilization support/matrix for industrially relevant enzymes. With this method, it is not only possible to immobilize pure enzymes; it can be also used for capturing recombinant enzymes directly out of culture supernatant. Therefore, the depth ...