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... In forest ecosystems, mineralization of native organic N provides the primary plant N nutrient source. The rate of this N mineralization is affected directly and indirectly by interactions with plant root biomass and is also probably impacted by interactions with the soil microbial community. Thus, the objectives of this study were to evaluate the relationship of N-mineralization rates in an acid ...
roots, etc ; Brassica juncea; salt tolerance; sodium chloride; somatic embryogenesis; embryo (plant); shoots; seed germination; proline; amino acid metabolism; esterases; isozymes; Show all 12 Subjects
Abstract:
... Somatic embryos, developed from hypocotyl segments of light-grown seedlings of Brassica juncea cv RLM198, were subjected to selection at varying concentrations of sodium chloride (NaCl). Plants were developed from proliferated somatic embryos selected on NaCl-containing medium. The selections were characterized for salt tolerance, esterase isozyme pattern, and proline accumulation. It has been fou ...
... Assimilation of ammonium (NH4) into free amino acids and total reduced nitrogen (N) was monitored in both roots and shoots of two-week old rice seedlings supplied with 5 mM 99% (15NH4)2SO4 in aerated hydroponic culture with or without a 2 h preincubation with 1 mM methionine sulfoximine (MSX) an inhibitor of glutamine synthetase (GS) activity. 15NH4 was not assimilated into amino acids when the GS ...
... Spinach plants were grown in hydroponic culture provided with variable limiting amounts of N. During a complete diurnal cycle, growth of the root and shoot parts, as well as levels of soluble and insoluble sugars and of free amino acids, were monitored. No clear relationship could be detected between the level of N feeding and the levels of free sugars and amino acids. Analysis of variance reveale ...
roots, etc ; drought; polyethylene glycol; potassium chloride; turgor; proline; water stress; sodium; cytochemistry; amino acid metabolism; leaves; Arabidopsis thaliana; seeds; potassium; sodium chloride; water potential; salinity; free amino acids; flowers; Show all 19 Subjects
Abstract:
... Significant differences were observed in the amount and proportion of free amino acids in different organs of Arabidopsis thaliana (L.) Heynh, ecotype Columbia. The most notable were found for proline, which formed 17-26% of the total free amino acid concentration in reproductive tissues (floret and seed), but only 1-3% of the total free amino acid concentration in vegetative tissues (rosette leaf ...
... Exogenous application of the purine precursor 5-aminoimidazole-4-carboxamide (AICA) riboside restores infection and enhances the development of bean (Phaseolus vulgaris) root nodules elicited by Rhizobium etli purine auxotrophs. The uniqueness of AICA riboside as an effector was shown by testing varying concentrations of AICA riboside and purines for this effect, and by examining several mutants d ...
... Proline is a common compatible osmolyte in higher plants. Proline accumulation in response to water stress and salinity is preceded by a rapid increase of the mRNA level of delta 1-pyrroline-5-carboxylate synthase (P5CS) controlling the rate-limiting step of glutamate-derived proline biosynthesis. P5CS is encoded by two differentially regulated genes in Arabidopsis. Gene AtP5CS1 mapped to chromoso ...
... The ability of ryegrass (Lolium perenne L.) to take up and utilize aspartic acid (Asp) and serine (Ser,), and the effect of colonization of the roots by the arbuscular mycorrhizal (AM) fungus Glomus fasciculatum (Thax. sensu Gerd.) were studied. The seedlings were grown under controlled conditions in a series of micro‐lysimeters. All plants were fed with a nutrient solution containing either nitra ...
roots, etc ; protein synthesis; Meloidogyne incognita; Gossypium; pest resistance; protein content; amino acid metabolism; infection; plant proteins; Show all 9 Subjects
Abstract:
... The root-knot nematode (RKN), Meloidogyne incognita (Kofoid and White) Chitwood, is a sedentary endoparasite that retards growth and development of cotton, Gossypium spp. L., by attacking the root system, causing galling, stunting, and other adverse effects. All commercial cultivars have susceptibility to RKN, although they vary in degree of susceptibility. Plant breeding has led to the developmen ...
... The physiological consequences for NO3(-) utilization by the plant of underexpression and overexpression of nitrate reductase (NR) were investigated in nine transformants of Nicotiana tabacum and Nicotiana plumbaginifolia. The in vitro NR activities (NRAs) in both roots and leaves of low- and high-NR tobacco transformants ranged from 5-10% to 150-200%, respectively, of those measured in wild-type ...
... Higher rates of nitrate assimilation are required to support faster growth in enhanced carbon dioxide. To investigate how this is achieved, tobacco plants were grown on high nitrate and high light in ambient and enhanced (700 micromoles mol-1) carbon dioxide. Surprisingly, enhanced carbon dioxide did not increase leaf nitrate reductase (NR) activity in the middle of the photoperiod. Possible reaso ...
... During the vegetation periods 1994 and 1995, net uptake of nitrate and ammonium by roots of adult spruce (Picea abies (L.) Karst) and beech (Fagus sylvatica L.) trees was studied at a field site exposed to high loads of N (‘Höglwald’, Germany). In addition, uptake experiments were carried out under controlled conditions with young spruce and beech trees grown at normal N supply. In the field, nitr ...
... Arabidopsis thaliana roots grow in a wavy pattern upon a slanted surface. A novel mutation in the anthranilate synthase alpha 1 (ASA1) gene, named trp5-2(wvc1), and mutations in the tryptophan synthase alpha and beta 1 genes (trp3-1 and trp2-1, respectively) confer a compressed root wave phenotype on tilted agar surfaces. When trp5-2(wvc1) seedlings are grown on media supplemented with anthranilat ...
roots, etc ; ammonium nitrate; sodium nitrate; application rate; nitrate reductase; salinity; phosphoenolpyruvate carboxylase; nitrogen; organic acids and salts; glutamate-ammonia ligase; cations; nutrient solutions; chemical constituents of plants; enzyme activity; amino acid metabolism; ammonium sulfate; Lolium multiflorum; shoots; Show all 18 Subjects
Abstract:
... The concentration of organic acids, organic nitrogen (N), nitrate (NO3), and total cations increased in annual ryegrass (Lolium multiflorum Lam.) with salinity and N concentration in the growth medium. Increasing salinity and N in the growth medium induced changes in the level of key enzymes of N assimilation and organic acids: nitrate reductase (NR, EC 1.6.6.1), phosphoenolpyruvate carboxylase (P ...
roots, etc ; gas exchange; ion transport; carbon; carbon dioxide; rhizosphere; nutrient uptake; electron transfer; anions; potassium cyanide; nutrient solutions; plasma membrane; amino acid metabolism; oxygen; phosphoenolpyruvate carboxylase; enzyme inhibitors; enzyme activity; Solanum lycopersicum var. lycopersicum; carbonate dehydratase; nutrient availability; biochemical pathways; nitrates; salicylic acids; Show all 23 Subjects
Abstract:
... Respiratory CO2 and O2 flux were measured in hydroponically grown Lycopersicon esculentum (L.) Mill. cv. F144 plants at either low (0 micromole mol-1) or elevated CO2 concentrations (>2000 micromole mol-1) supplied to the roots. In NO2--fed plants the consumption of O2 and the engagement of the alternative pathway were increased by elevated dissolved inorganic carbon (DIC=CO2+ HCO3-) concentration ...
roots, etc ; nitrate reductase; malic acid; aspartate-ammonia ligase; starch; varieties; gene expression; amino nitrogen; hexoses; amino acid metabolism; root shoot ratio; leaves; Zea mays; messenger RNA; seeds; sucrose; strain differences; translocation (plant physiology); phloem; xylem; enzyme activity; asparagine; chemical constituents of plants; protein content; Show all 24 Subjects
Abstract:
... In order to understand the metabolic processes governing the protein content in maize grains, we compared metabolic parameters of the two maize strains Illinois High Protein (IHP) and Illinois Low Protein (ILP), which differ largely in their relative starch and protein content. The activity of nitrate reductase (NR) and the transcription level of asparagine synthetase, two enzymes that play a cent ...
roots, etc ; Zea mays; biosynthesis; isotope labeling; water potential; seedlings; ornithine; proline; histochemistry; active transport; amino acid metabolism; glutamic acid; Show all 12 Subjects
Abstract:
... The proline (Pro) concentration increases greatly in the growing region of maize (Zea mays L.) primary roots at low water potentials (psi(w)), largely as a result of an increased net rate of Pro deposition. Labeled glutamate (Glu), ornithine (Orn), or Pro was supplied specifically to the root tip of intact seedlings in solution culture at high and low psi(w) to assess the relative importance of Pr ...
... A cDNA clone that encodes a feedback-insensitive anthranilate synthase (AS), ASA2, isolated from a 5-methyl-tryptophan (Trp) (5MT)-resistant tobacco cellline under the control of the constitutive cauliflower mosaic virus 35S promoter, was introduced into the forage legume Astragalus sinicus by Agrobacterium rhizogenes with kanamycin selection. The 35S-ASA2 gene was expressed constitutively as demo ...
... Using a transposon carrying a promoterless lux operon to generate transcriptional fusions by insertional mutagenesis, we have identified a Pseudomonas putida gene with increased expression in the presence of corn root exudates. Expression of the transcriptional fusion, induced by the amino acid lysine, was detected in P. putida in the rhizosphere of plants as well as in response to seed exudates. ...
roots, etc ; seed germination; Juglans regia; stems; free amino acids; seedlings; nutrient availability; citrulline; alanine; cotyledons; embryo (animal); translocation (plant physiology); chemical constituents of plants; xylem; amino acid metabolism; leaves; Show all 16 Subjects
Abstract:
... We investigated the composition of free amino acids in walnut (Juglans regia L.) seeds (embryo and cotyledons). We also examined xylem transport of free amino acids in young seedlings grown in the absence of external nutrients. A relatively high concentration of free alanine was found in seed tissue, whereas a relatively high concentration of citrulline was detected in young seedlings. Citrulline ...
... The primary flavor compound in potato, methional, is synthesized from methionine by the Strecker degradation reaction. A major problem associated with potato processing is the loss of methional. Methional or its precursor, methionine, is not added back during potato processing due to high costs of production. A novel approach to enhance the methional level in processed potato would be to increase ...
... Anthranilate synthase (AS) is a key enzyme in the biosynthesis of various indole compounds including tryptophan. AS consists of two subunits, alpha and beta, and converts chorismate to anthranilate. Two or more AS alpha-subunit genes have been identified and characterized in several land plants. Although alpha subunits of AS induced by elicitation have been suggested to play significant roles in s ...
... In cucumber (Cucumis sativus L.), phosphoenolpyruvate carboxykinase (PEPCK) was shown by activity measurements and immunoblots to be present in leaves, stems, roots, flowers, fruit and seed. However, immunolocalisation showed that it was present only in certain cell types. PEPCK was present in the companion cells of the adaxial phloem of minor veins, the adaxial and abaxial phloem of larger veins, ...
roots, etc ; messenger RNA; pentosyltransferases; complementary DNA; Alyssum montanum; gene expression; nucleotide sequences; nickel; metal tolerance; amino acid metabolism; histidine; hyperaccumulators; Show all 12 Subjects
Abstract:
... Plants that hyperaccumulate Ni exhibit an exceptional degree of Ni tolerance and the ability to translocate Ni in large amounts from root to shoot. In hyperaccumulator plants in the genus Alyssum, free His is an important Ni binding ligand that increases in the xylem proportionately to root Ni uptake. To determine the molecular basis of the His response and its contribution to Ni tolerance, transc ...
... Storage protein synthesis is dependent on available nitrogen in the seed, which may be controlled by amino acid import via specific transporters. To analyze their rate-limiting role for seed protein synthesis, a Vicia faba amino acid permease, VfAAP1, has been ectopically expressed in pea (Pisum sativum) and Vicia narbonensis seeds under the control of the legumin B4 promoter. In mature seeds, sta ...
... The inhibition of branched-chain amino acid (BCAA) biosynthesis was evaluated in pea plants in relation to the ability for induction of fermentative metabolism under aerobic conditions. Chlorsulfuron and imazethapyr (inhibitors of acetolactate synthase, ALS, EC 4.1.3.18) produced a strong induction of pyruvate decarboxylase (PDC, EC 4.1.1.1) and alcohol dehydrogenase (ADH, EC 1.1.1.1) activities a ...
... The pattern of nitrogen assimilation in soybean plants treated with a herbicide that inhibits branched-chain amino acid biosynthesis was evaluated by 15N isotopic analysis. The herbicide imazethapyr caused a strong decrease in nitrate uptake by roots, partly due to a reduced stomatal conductance. The inhibition of 15N uptake was accompanied by a decrease in the 15N content in the plant and, concom ...
roots, etc ; ammonia; ion channels; glutamate dehydrogenase; soil nutrients; transaminases; arginine; deamination; glutamate synthase (NADH); glutamate-ammonia ligase; biochemical pathways; glutamates; signal transduction; genes; receptors; amino acid metabolism; glutamic acid; soil-plant interactions; Show all 18 Subjects
Abstract:
... Glutamate occupies a central position in amino acid metabolism in plants. The acidic amino acid is formed by the action of glutamate synthase, utilizing glutamine and 2-oxoglutarate. However, glutamate is also the substrate for the synthesis of glutamine from ammonia, catalysed by glutamine synthetase. The α-amino group of glutamate may be transferred to other amino acids by the action of a wide r ...
roots, etc ; ionization; seedlings; plant pathogens; genes; mass spectrometry; shoots; two-dimensional gel electrophoresis; plant diseases and disorders; proteome; cell walls; biological control agents; starch; root inoculation; disease resistance; photosynthesis; Trichoderma lixii; gene expression regulation; amino acid metabolism; plant growth; Zea mays; corn; induced resistance; proteins; protein synthesis; stress response; desorption; Show all 27 Subjects
Abstract:
... Trichoderma spp. are effective biocontrol agents for several soil-borne plant pathogens, and some are also known for their abilities to enhance systemic resistance to plant diseases and overall plant growth. Root colonization with Trichoderma harzianum Rifai strain 22 (T22) induces large changes in the proteome of shoots of maize (Zea mays) seedlings, even though T22 is present only on roots. We c ...
... In addition to serving as an important nitrogen reserve, l-Arginine participates in various physiological processes in plants. In plants, like in animals, nitric oxide (NO) is synthesized through nitric oxide synthase (NOS) pathway, while polyamines (PAs) are through arginine decarboxylase (ADC) and/or arginase-ornithine decarboxylase (ODC) pathway in arginine metabolism. In this study, arginine m ...
roots, etc ; tomatoes; plant viruses; plant tissues; plant diseases and disorders; ethylene; microarray technology; shoots; signal transduction; transcription (genetics); gene expression; secondary metabolites; resistance mechanisms; abscisic acid; amino acid metabolism; Tomato spotted wilt orthotospovirus; Solanum lycopersicum var. lycopersicum; Show all 17 Subjects
Abstract:
... Tomato (Solanum lycopersicon), a model species for the family Solanaceae, is severely affected by Tomato spotted wilt virus (TSWV) worldwide. To elucidate the systemic transcriptional response of plants to TSWV infection, microarray experiments were performed on tomato. Parallel analysis of both shoots and roots revealed organ-specific responses, although the virus was present in similar concentra ...
... The waterlogging tolerance and the physiological responses to this stress were tested in seedlings of Fraxinus angustifolia, an ash tree inhabiting riparian forests, and two provenances of the closely related Fraxinus excelsior, one derived from a riparian forest (FER) and one from a mountainous region (FEM). Besides visible damage, physiological parameters reflecting adaptations of plants to wate ...
... The molecular and physiological responses of gray poplar (Populus x canescens) following root hypoxia were studied in roots and leaves using transcript and metabolite profiling. The results indicate that there were changes in metabolite levels in both organs, but changes in transcript abundance were restricted to the roots. In roots, starch and sucrose degradation were altered under hypoxia, and c ...
... Germination of buckwheat seeds in solutions of synthetic mono- and tricyclic cyclopentane β,β'-triketones of various concentrations was accompanied by inhibition of seedling root growth and changes in the contents of glutamate, γ-aminobutyric acid, proline, glutamine, and alanine. The monocyclic triketone also affected the amount of isoleucine. It is likely that the increase in proline content is ...
Afzal, Ahmed J.; Natarajan, Aparna; Saini, Navinder; Iqbal, M. Javed; Geisler, Matt; El Shemy, Hany A.; Mungur, Rajsree; Willmitzer, Lothar; Lightfoot, David A.
roots, etc ; Glycine max; Heterodera glycines; alleles; amino acid metabolism; chronic diseases; gas chromatography; giant cells; glucose-6-phosphate isomerase; isogenic lines; loci; mass spectrometry; metabolites; protein degradation; proteins; proteome; soybeans; systemic acquired resistance; trypsin; two-dimensional gel electrophoresis; Show all 20 Subjects
Abstract:
... Heterodera glycines, the soybean cyst nematode (SCN), causes the most damaging chronic disease of soybean (Glycine max). Host resistance requires the resistance allele at rhg1. Resistance destroys the giant cells created in the plant's roots by the nematodes about 24 to 48 h after commencement of feeding. In addition, 4 to 8 d later, a systemic acquired resistance develops that discourages later i ...
... Typical for a graminaceous plant, barley secretes mugineic acid-family phytosiderophores (MAs) to acquire iron (Fe). Under Fe-deficient conditions, MAs secretion from barley roots increases markedly. Secretion shows a diurnal pattern, with a clear peak 2-3 h after sunrise and cessation within a few hours. Microarray analyses were performed to profile the Fe deficiency-inducible genes in barley roo ...
roots, etc ; Malus hupehensis; amino acid metabolism; amino acids; carbohydrate metabolism; complementary DNA; genes; leaves; nucleotides; open reading frames; quantitative polymerase chain reaction; rapid amplification of cDNA ends; starch; stems; tomatoes; Show all 15 Subjects
Abstract:
... Sucrose non-fermenting-1-related protein kinase-1 (SnRK1) plays an important role in metabolic regulation in plant. To understand the molecular mechanism of amino acids and carbohydrate metabolism in Malus hupehensis Rehd. var. pinyiensis Jiang (Pingyi Tiancha, PYTC), a full-length cDNA clone encoding homologue of SnRK1 was isolated from PYTC by Rapid Amplification of cDNA Ends (RACE). The clone, ...
roots, etc ; amino acid metabolism; cell walls; corn; flavonoids; phenolic compounds; proteomics; reactive oxygen species; root growth; soy protein; soybeans; transcriptomics; water stress; Show all 13 Subjects
Abstract:
... Progress in understanding root growth regulation and adaptation under water-stressed conditions is reviewed, with emphasis on recent advances from transcriptomic and proteomic analyses of maize and soybean primary roots. In both systems, kinematic characterization of the spatial patterns of cell expansion within the root elongation zone showed that at low water potentials, elongation rates are pre ...
... Conifers have a preference for ammonium over nitrate as the main inorganic nitrogen source. However, it is unknown how changes in nitrogen nutrition may affect transcription profiles. In this study, microarray analysis and suppressive subtraction hybridization were used to identify differentially expressed genes in the roots of maritime pine exposed to changes in ammonium availability. A total of ...
roots, etc ; Zea mays; grain crops; phytotoxicity; metabolic detoxification; corn; transcription (genetics); biochemical pathways; water stress; metal tolerance; aluminum; inbred lines; type III secretion system; gene expression regulation; amino acid metabolism; leaves; Show all 16 Subjects
Abstract:
... So far, the tolerance mechanisms of plant tolerance to aluminium (Al) toxicity are still controversial. This will require an interdisciplinary approaches integrating genetic, molecular, and physiological investigations. To lay a foundation for studying mechanism of whole maize plant tolerance to Al stress (AS) and growth recovery by removal of Al toxicity (RAT), temporal physiological responses an ...
... Plants reconfigure their metabolic network under stress conditions. Changes of mitochondrial metabolism such as tricarboxylic acid (TCA) cycle and amino acid metabolism are reported in Arabidopsis roots but the exact molecular basis underlying this remains unknown. We here hypothesise the reassembly of enzyme protein complexes to be a molecular mechanism for metabolic regulation and tried in the p ...