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You searched for: Subject term amino acids Remove constraint Subject term: amino acids Subject term Crassostrea gigas Remove constraint Subject term: Crassostrea gigas
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1. Integrated application of transcriptomics and metabolomics provides insights into condition index difference mechanisms in the Pacific oyster (Crassostrea gigas)

Author:
Xin He; Fucun Wu; Luping Wang; Li Li; Guofan Zhang
Source:
Genomics 2022 v.114 no.4 pp. 110413
ISSN:
0888-7543
Subject:
Crassostrea gigas; amino acids; carbohydrates; energy metabolism; genomics; lipids; metabolomics; transcriptomics
Abstract:
... The condition index (CI) is an economically important tool for assessing the quality of oysters, such as the Pacific oyster Crassostrea gigas. However, little is known about the mechanisms that underlie differences in CI between different C. gigas populations. In this study, we integrated transcriptomic and metabolomic profiling to investigate the mechanisms that underlie the differences between h ...
DOI:
10.1016/j.ygeno.2022.110413

2. Physicochemical characterization and identification of major linear epitopes of sarcoplasmic calcium‐binding protein (SCP) allergen from Pacific oyster (Crassostrea gigas)

Author:
Qingli Cheng; Xiaowen Feng; Xiaohan Zhao; Ruizeng Gu; Jun Lu; Wenying Liu; Guoming Li
Source:
Journal of the science of food and agriculture 2022 v.102 no.9 pp. 3551-3562
ISSN:
0022-5142
Subject:
Crassostrea gigas; agriculture; allergens; amino acids; blood serum; calcium-binding proteins; cholera toxin; digestion; enzyme-linked immunosorbent assay; food allergies; immune response; immunodominant epitopes; immunoglobulin E; models; molecular weight; muscles; oysters; pepsin; peptides; rats; shellfish; trypsin
Abstract:
... BACKGROUND: Food allergy is a serious public nutritional health problem that has attracted extensive worldwide attention. Shellfish allergy is a long‐lasting disorder that has a lifelong impact on health. Sarcoplasmic calcium‐binding protein (SCP) plays a vital role in cell and muscle functions and has been identified as an allergen in oyster. RESULTS: In this study, recombinant SCP (rSCP) with a ...
DOI:
10.1002/jsfa.11699

3. Winogradskyella luteola sp.nov., Erythrobacter ani sp. nov., and Erythrobacter crassostrea sp.nov., isolated from the hemolymph of the Pacific Oyster Crassostrea gigas

Author:
Hani Pira; Chandra Risdian; Mathias Müsken; Peter J. Schupp; Joachim Wink
Source:
Archives of microbiology 2022 v.204 no.8 pp. 488
ISSN:
0302-8933
Subject:
Crassostrea gigas; Erythrobacter; Sudan dyes; amino acids; bioremediation; coenzyme Q10; hemolymph; microbiology; nucleotide sequences; sequence analysis
Abstract:
... Three new bacterial strains, WHY3ᵀ, WH131ᵀ, and WH158ᵀ, were isolated and described from the hemolymph of the Pacific oyster Crassostrea gigas utilizing polyphasic taxonomic techniques. The 16S rRNA gene sequence analysis revealed that strain WHY3ᵀ was a member of the genus Winogradskyella, whereas strains WHI31ᵀ and WH158ᵀ were members of the genus Erythrobacter. According to the polygenomic stud ...
DOI:
10.1007/s00203-022-03099-y

4. Comparison of amino acid, 5′-nucleotide and lipid metabolism of oysters (Crassostrea gigas Thunberg) captured in different seasons

Author:
Jing-Xuan Gao; Yu-Ying Zhang; Xu-Hui Huang; Rong Liu; Xiu-Ping Dong; Bei-Wei Zhu; Lei Qin
Source:
Food research international 2021 v.147 pp. 110560
ISSN:
0963-9969
Subject:
Crassostrea gigas; amino acids; aquaculture; diacylglycerols; food research; lipid composition; lipid metabolism; lysophosphatidylcholine; muscles; nutritive value; oysters; phosphatidylethanolamines; product development; shellfish; stable isotopes; tandem mass spectrometry; taste; triacylglycerols
Abstract:
... As an important aquaculture shellfish, the superior taste and high nutritional value of oyster (Crassostrea gigas Thunberg) have drawn extensive attention. In this study, twenty-one free amino acids (FAAs) and six 5′-nucleotides were evaluated through stable isotope labeling-liquid chromatography coupled with tandem mass spectrometry (SIL-LC-MS/MS), and the lipid profile was explored using ultrahi ...
DOI:
10.1016/j.foodres.2021.110560

5. The DNA cytosine-5-methyltransferase 3 (DNMT3) involved in regulation of CgIL-17 expression in the immune response of oyster Crassostrea gigas

Author:
Qi Zhao; Weilin Wang; Jia xin Li; Pei Yuan; Yu Liu; Yan Li; Lingling Wang; Linsheng Song
Source:
Developmental and comparative immunology 2021 v.123 pp. 104092
ISSN:
0145-305X
Subject:
5-methylcytosine; Crassostrea gigas; DNA; DNA methylation; DNA methyltransferase; Vibrio splendidus; amino acids; cytokines; cytoplasm; genomics; hemocytes; immune response; muscles; oysters; transposons
Abstract:
... DNA methyltransferase, a key enzyme mediating DNA methylation, is involved in numerous processes including genomic imprinting, X chromosome inactivation, transposable element suppression, and immune defense in vertebrates. In the present study, a DNA cytosine-5-methyltransferase 3 was identified from oyster Crassostrea gigas (designed as CgDNMT3). There were a PWWP domain, a PHD domain and a DNA-m ...
DOI:
10.1016/j.dci.2021.104092

6. Pacificimonas pallium sp. nov., an Isolated Bacterium from the Mantle of Pacific Oyster Crassostrea gigas in Germany, and Prediction of One-Carbon Metabolism

Author:
Hani Pira; Chandra Risdian; Mathias Müsken; Peter J. Schupp; Joachim Wink
Source:
Diversity 2022 v.14 no.3 pp. -
ISSN:
1424-2818
Subject:
Candida albicans; Crassostrea gigas; agar; amino acids; bacteria; coenzyme Q10; glycosphingolipids; metabolism; nucleotide sequences; phosphatidylethanolamines; polyhydroxyalkanoates; prediction; siderophores; spermidine; spermine; vitamin B12; Germany
Abstract:
... A yellow bacterium from marine agar, strain WHA3ᵀ, was isolated from the mantel of the Pacific oysters Crassostrea gigas in the Wilhelmshaven Sea in northern Germany. Based on the 16S rRNA gene sequence, strain WHA3ᵀ had a high similarity to Pacificimonas flava JLT2015ᵀ (95.80%) and 94.79% to Pacificimonas aurantium JLT2012ᵀ. Furthermore, the dDDH and ANI value analysis between WHA3ᵀ and other clo ...
DOI:
10.3390/d14030181

7. UPLC-MS metabolomics provides insights into the differences between black- and white-shelled Pacific oysters Crassostrea gigas

Author:
Xi Chen; Qiuyun Jiang; Hongce Song; Lingling Li; Chaoyi Xie; Baoyu Huang; Yaqiong Liu; Meiwei Zhang; Lei Wei; Xiaotong Wang
Source:
Journal of oceanology and limnology 2021 v.39 no.1 pp. 340-349
ISSN:
2096-5508
Subject:
Crassostrea gigas; amino acids; glycerophospholipids; limnology; lipid content; melanin; metabolism; metabolites; metabolomics; nutritive value; oceanography
Abstract:
... A variety of shell colors are one of the most fundamental characteristics of molluscs, which have importantly ecological and economic significance. The Pacific oyster Crassostrea gigas is distributed in many sea areas around the world and also an aquacultured mollusc with high nutritional value. In this study, the whole soft body and the mantle tissue of black-shelled Pacific oyster (BSO) and whit ...
DOI:
10.1007/s00343-020-0117-3

8. A myxovirus resistance like protein involved in CgIFNLP mediated immune response of oyster Crassostrea gigas

Author:
Yuanmei Li; Xue Qiao; Zhaoqun Liu; Lingling Wang; Linsheng Song
Source:
Fish & shellfish immunology 2021 v.119 pp. 318-328
ISSN:
1050-4648
Subject:
Crassostrea gigas; amino acid sequences; amino acids; cytoplasm; dynamins; fish; gene expression; hemocytes; immune response; interferons; oysters; palps; peptides; phylogeny; shellfish; signal transduction; transactivators
Abstract:
... The myxovirus resistance (Mx) proteins belong to interferon (IFN)-induced dynamin GTPase and play a pivotal role in the inhibition of replication of numerous viruses. In the present study, an Mx homologue (designated as CgMx1) was identified from oyster Crassostrea gigas. The open reading frame (ORF) of CgMx1 cDNA was of 1689 bp encoding a peptide of 562 amino acid residues. There was an N-termina ...
DOI:
10.1016/j.fsi.2021.10.008

9. A tripartite motif protein (CgTRIM1) involved in CgIFNLP mediated antiviral immunity in the Pacific oyster Crassostrea gigas

Author:
Jihan Wang; Xue Qiao; Zhaoqun Liu; Yuting Wang; Yuanmei Li; Yage Liang; Chang Liu; Lingling Wang; Linsheng Song
Source:
Developmental and comparative immunology 2021 v.123 pp. 104146
ISSN:
0145-305X
Subject:
Crassostrea gigas; RNA interference; amino acids; gene expression; hemocytes; immunity; inflammation; oysters; polypeptides; ubiquitin-protein ligase
Abstract:
... Tripartite motif (TRIM) proteins are a large family of E3 ubiquitin ligases involved in many biological processes, such as inflammation and antiviral immunity. In the present study, a novel TRIM protein homolog named CgTRIM1 was identified from Pacific oyster Crassostrea gigas. The open reading frame (ORF) of CgTRIM1 was of 1914 bp encoding a putative polypeptide of 637 amino acid residues. There ...
DOI:
10.1016/j.dci.2021.104146

10. Identification and characterization of an apoptosis-inducing factor 1 involved in apoptosis and immune defense of oyster, Crassostrea gigas

Author:
Xue Qiao; Lilin Hou; Jihan Wang; Yuhao Jin; Ning Kong; Jialuo Li; Sicong Wang; Lingling Wang; Linsheng Song
Source:
Fish & shellfish immunology 2021 v.119 pp. 173-181
ISSN:
1050-4648
Subject:
Crassostrea gigas; amino acid sequences; amino acids; apoptosis; cell viability; cytoplasm; fish; hemocytes; immune response; insects; mortality; oysters; peptides; phosphatidylserines; phylogeny; plasmids; shellfish; transfection
Abstract:
... The apoptosis-inducing factor (AIF) is a phylogenetically old protein with classic function of inducing caspase-independent apoptosis, which extensively present in all primary kingdoms. In the present study, an AIF homologue (designated as CgAIF1) was identified from oyster Crassostrea gigas. The open reading frame of CgAIF1 cDNA was of 1836 bp encoding a peptide of 611 amino acid residues. There ...
DOI:
10.1016/j.fsi.2021.09.016

11. Molecular cloning and expression analysis of mc5r like genes (mc5rl) in Ruditapes philippinarum (Manila clam) after aerial exposure and low-temperature stress

Author:
Kunyin Jiang; Hongtao Nie; Xiwu Yan
Source:
Molecular biology reports 2020 v.47 no.11 pp. 8891-8901
ISSN:
0301-4851
Subject:
Crassostrea gigas; Crassostrea virginica; Mizuhopecten yessoensis; Pecten maximus; Ruditapes philippinarum; amino acids; cold stress; genes; hepatopancreas; lipid metabolism; molecular biology; obesity; phylogeny; stress response; tissue distribution; vertebrates
Abstract:
... The melanocortin-5 receptor (mc5r) plays an important role in exocrine function, lipid metabolism, obesity, and stress response in the vertebrate. However, the functions of the mc5r in mollusks have been rarely investigated. We cloned the full length of Ruditapes philippinarum mc5r like gene (mc5rl) and the sequence structure and phylogenetic relationship of mc5rl were analyzed. Besides, we detect ...
DOI:
10.1007/s11033-020-05941-2

12. Molecular cloning and immune function study of an oyster IκB gene in the NF-κB signaling pathway

Author:
Xiuxiu Sang; Juan Dong; Fengmei Chen; Lei Wei; Yaqiong Liu; Meiwei Zhang; Baoyu Huang; Xiaotong Wang
Source:
Aquaculture 2020 v.525 pp. 735322
ISSN:
0044-8486
Subject:
Crassostrea gigas; DNA; amino acids; breeding; disease resistance; enzymes; genes; immune response; industry; innate immunity; messenger RNA; molecular cloning; mortality; open reading frames; oysters; pathogens; phylogeny; quantitative polymerase chain reaction; signal transduction; stomach; tissues; transcription factor NF-kappa B; tumor necrosis factor-alpha
Abstract:
... Mass mortality caused by pathogens has severely affected the oyster cultivation industry, but our knowledge about the innate immune system of oysters is still limited. The nuclear factor-κB (NF-κB) signaling pathway plays a vital role in many cellular, developmental, and biological processes. And inhibitor of NF-κB (IκB) proteins can prevent NF-κB nuclear localization and DNA binding, thereby inhi ...
DOI:
10.1016/j.aquaculture.2020.735322

13. Molluscan Beclin-1 is involved in the innate immune response by regulating the autophagosomes formation in Crassostrea hongkongensis

Author:
Yinyin Zhou; Kunna Liu; Xingyou Li; Yanpin Qin; Yuehuan Zhang; Yang Zhang; Zhiming Xiang; Haitao Ma; Jun Li; Ziniu Yu
Source:
Aquaculture reports 2020 v.18 pp. 100541
ISSN:
2352-5134
Subject:
Crassostrea gigas; Crassostrea hongkongensis; Crassostrea virginica; Mizuhopecten yessoensis; RNA interference; amino acid sequences; amino acids; autophagosomes; autophagy; complementary DNA; dose response; endoplasmic reticulum; enzymes; gene overexpression; gills; innate immunity; isoelectric point; mammals; messenger RNA; molecular weight; molluscs; phylogeny; reporter genes; sequence homology; tissues; transcription (genetics); transcription factor NF-kappa B
Abstract:
... Beclin-1 plays a central role in autophagy, which is well documented in mammals. However, the identity and function of Beclin-1 remain largely unclear in mollusks. In this study, a Beclin-1 gene (ChBeclin-1) was identified and characterized from Crassostrea hongkongensis. The full length of ChBeclin-1 cDNA encoded a predicted 442-amino acid protein with a deduced molecular weight of 51.11 kDa and ...
DOI:
10.1016/j.aqrep.2020.100541

14. PmCBP, a novel poly (chitin-binding domain) gene, participates in nacreous layer formation of Pinctada fucata martensii

Author:
Shanshan Fan; Zhe Zheng; Ruijuan Hao; Xiaodong Du; Yu Jiao; Ronglian Huang
Source:
Comparative biochemistry and physiology 2020 v.240 pp. 110374
ISSN:
1096-4959
Subject:
3' untranslated regions; Crassostrea gigas; Mizuhopecten yessoensis; Pinctada fucata; RNA interference; amino acids; chitin; genes; growth traits; in situ hybridization; microstructure; nacre; oysters; phylogeny; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction
Abstract:
... Chitin participates in shell formation as the main component of an organic framework. Chitin-binding protein contains domains that can bind to chitin specifically. In this study, a novel chitin-binding protein from Pinctada fucata martensii (PmCBP) with poly (chitin-binding domain) was cloned, which contains a 5′-untranslated region (UTR) of 114 bp and 3′UTR of 116 bp, and encodes a putative prote ...
DOI:
10.1016/j.cbpb.2019.110374

15. Zooshikella harenae sp. nov., Isolated from Pacific Oyster Crassostrea gigas, and Establishment of Zooshikella ganghwensis subsp. marina subsp. nov. and Zooshikella ganghwensis subsp. ganghwensis subsp. nov.

Author:
Hani Pira; Chandra Risdian; Peter Kämpfer; Mathias Müsken; Peter J. Schupp; Joachim Wink
Source:
Diversity 2021 v.13 no.12 pp. -
ISSN:
1424-2818
Subject:
Crassostrea gigas; DNA; amino acids; antimicrobial properties; genome; hybridization; lipid composition; menaquinones; new subspecies; phosphatidylethanolamines; phylogeny; sand; Germany
Abstract:
... Here, we describe the polyphasic taxonomy of a novel isolated strain WH53ᵀ from the genus Zooshikella isolated from the sand sediment located between the lumen of the Crassostrea gigas From Germany. Phylogenetic analysis determined that the strain WH53ᵀ had a high similarity to Zooshikella ganghwensis JC2044ᵀ (99.57%) and Zooshikella marina LMG 28823ᵀ (99.36%). Strain WH53ᵀ contained ubiquinone-9 ...
DOI:
10.3390/d13120641

16. A calmodulin targeted by miRNA scaffold659_26519 regulates IL-17 expression in the early immune response of oyster Crassostrea gigas

Author:
Zirong Han; Jialuo Li; Weilin Wang; Jiaxin Li; Qi Zhao; Meijia Li; Lingling Wang; Linsheng Song
Source:
Developmental and comparative immunology 2021 v.124 pp. 104180
ISSN:
0145-305X
Subject:
Crassostrea gigas; amino acids; calcium; calmodulin; gene expression; hemocytes; hepatopancreas; immune response; inflammation; luciferase; microRNA; muscles; oysters; polypeptides; transfection
Abstract:
... Calmodulin (CaM) is a highly conserved second messenger protein transducing calcium signals by binding and modulating intracellular calcium ions (Ca²⁺), and involves in the Ca²⁺-dependent physical processes including host defense in vertebrates. In the present study, a CaM homologue (designated as CgCaM) was identified from Pacific oyster Crassostrea gigas. The open reading frame of CgCaM cDNA was ...
DOI:
10.1016/j.dci.2021.104180

17. A novel Adiponectin receptor (AdipoR) involved in regulating cytokines production and apoptosis of haemocytes in oyster Crassostrea gigas

Author:
Wenjing Ge; Shu Huang; Shujing Liu; Jiejie Sun; Zhaoqun Liu; Wenwen Yang; Lingling Wang; Linsheng Song
Source:
Developmental and comparative immunology 2020 v.110 pp. 103727
ISSN:
0145-305X
Subject:
B-lymphocytes; Crassostrea gigas; Vibrio splendidus; adiponectin; adiponectin receptors; amino acid sequences; amino acids; apoptosis; calcium; complementary DNA; gene expression; gene expression regulation; hemocytes; hepatopancreas; immune response; interleukins; lipopolysaccharides; messenger RNA; oysters; polyinosinic-polycytidylic acid; polypeptides; transfection; vertebrates
Abstract:
... Adiponectin receptors (AdipoRs) comprise a seven-transmembrane domain-containing protein family, which specifically recognize adiponectin (APN) and play critical roles in the immunological and physiological processes in vertebrates. In the present study, a novel AdipoR is identified from oyster Crassostrea gigas (designated as CgAdipoR). The full-length cDNA of CgAdipoR is of 1209 bp encoding a po ...
DOI:
10.1016/j.dci.2020.103727

18. A novel CgIFNLP receptor involved in regulating ISG expression in oyster Crassostrea gigas

Author:
Xue Qiao; Yanan Zong; Zhaoqun Liu; Yuanmei Li; Jihan Wang; Lingling Wang; Linsheng Song
Source:
Developmental and comparative immunology 2021 v.124 pp. 104206
ISSN:
0145-305X
Subject:
Crassostrea gigas; amino acids; cytoplasm; fibronectins; gene expression; hemocytes; immunity; oysters; peptides; transcription (genetics)
Abstract:
... Interferons (IFNs) are the key coordinators of antiviral immunity by binding to their receptors to orchestrate a complex transcriptional network in vertebrates. Recently, the existence of molluscan IFN-like system has been certified by the identification of important components in IFN system, such as IFN-like protein (CgIFNLP) from oyster Crassostrea gigas. In the present study, a novel CgIFNLP re ...
DOI:
10.1016/j.dci.2021.104206

19. AP-1 regulates the expression of IL17-4 and IL17-5 in the pacific oyster Crassostrea gigas

Author:
Liyan Wang; Jiejie Sun; Zhaojun Wu; Xingye Lian; Shuo Han; Shu Huang; Chuanyan Yang; Lingling Wang; Linsheng Song
Source:
Fish & shellfish immunology 2020 v.97 pp. 554-563
ISSN:
1050-4648
Subject:
Crassostrea gigas; Crassostrea hongkongensis; amino acids; bacterial infections; gene expression; gene expression regulation; genes; granulocytes; hemocytes; immune response; leucine zipper; lipopolysaccharides; messenger RNA; oysters; phylogeny; tissues
Abstract:
... The activator protein-1 (AP-1) plays an important role in inducing the immune effector production in response to cellular stress and bacterial infection. In the present study, an AP-1 was identified from Pacific oyster Crassostrea gigas (designed as CgAP-1) and its function was investigated in response against lipopolysaccharide (LPS) stimulation. CgAP-1 was consisted of 290 amino acids including ...
DOI:
10.1016/j.fsi.2019.12.080

20. ATG10 (autophagy-related 10) regulates the formation of autophagosome in the anti-virus immune response of pacific oyster (Crassostrea gigas)

Author:
Zirong Han; Weilin Wang; Xiaojing Lv; Yanan Zong; Shujing Liu; Zhaoqun Liu; Lingling Wang; Linsheng Song
Source:
Fish & shellfish immunology 2019 v.91 pp. 325-332
ISSN:
1050-4648
Subject:
Crassostrea gigas; Crassostrea virginica; Octopodidae; amino acids; autophagy; cell viability; complementary DNA; double-stranded RNA; gene expression; gene expression regulation; gills; hemocytes; hepatopancreas; immune response; interferon regulatory factors; interferons; messenger RNA; open reading frames; oysters; polypeptides; shellfish
Abstract:
... Autophagy, a highly conserved intracellular degradation system, is involved in numerous processes in vertebrate and invertebrate, such as cell survival, ageing, and immune responses. However, the detailed molecular mechanism of autophagy and its immune regulatory role in bivalves are still not well understood. In the present study, an autophagy-related protein ATG10 (designated as CgATG10) was ide ...
DOI:
10.1016/j.fsi.2019.05.027

21. A C1qDC (CgC1qDC-6) with a collagen-like domain mediates hemocyte phagocytosis and migration in oysters

Author:
Huan Li; Ning Kong; Jiejie Sun; Weilin Wang; Meijia Li; Changhao Gong; Miren Dong; Min Wang; Lingling Wang; Linsheng Song
Source:
Developmental and comparative immunology 2019 v.98 pp. 157-165
ISSN:
0145-305X
Subject:
Crassostrea gigas; Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; Komagataella pastoris; Micrococcus luteus; Staphylococcus aureus; Vibrio splendidus; amino acids; chemotaxis; collagen; dose response; fungi; hemocytes; hepatopancreas; immune response; mannose; messenger RNA; muscles; oligomerization; oysters; pathogen-associated molecular patterns; pathogens; peptides; phagocytosis; phylogeny; polyinosinic-polycytidylic acid; protein content; signal peptide; tissues; vertebrates
Abstract:
... Most of the bivalve C1q domain containing proteins (C1qDCs) are either only composed of the globular head domain, or contain an N-terminal coiled-coil domain, presumed to cover a role in oligomerization. On the other hand, collagen regions, widespread in vertebrate C1qDCs, are very uncommon in bivalves. In the present study, a C1qDC with a collagen-like domain (designated CgC1qDC-6) was identified ...
DOI:
10.1016/j.dci.2019.04.010

22. CgSOCS6 negatively regulates the expression of CgIL17s and CgDefh1 in the pacific oyster Crassostrea gigas

Author:
Zhaojun Wu; Jiejie Sun; Liyan Wang; Yanan Zong; Zirong Han; Wen Yang; Zhaoqun Liu; Lingling Wang; Linsheng Song
Source:
Fish & shellfish immunology 2019 v.93 pp. 1084-1092
ISSN:
1050-4648
Subject:
Crassostrea gigas; Eriocheir sinensis; amino acid sequences; amino acids; complementary DNA; gene expression; gene expression regulation; hemocytes; immune response; interleukins; lipopolysaccharides; messenger RNA; open reading frames; oysters; phylogeny; shellfish; signal transduction
Abstract:
... As a family of negatively feedback regulating factors, the suppressor of cytokine signaling (SOCS) can depress cytokine signal transduction, and eventually modulate growth, development, differentiation, and immune response. In the present study, a SOCS homologue (designated as CgSOCS6) was identified from oyster Crassostrea gigas. The open reading frame of CgSOCS6 cDNA was of 1167 bp encoding a pe ...
DOI:
10.1016/j.fsi.2019.08.055

23. IKKε-like plays an important role in the innate immune signaling of the Pacific oyster (Crassostrea gigas)

Author:
Baoyu Huang; Xueying Tang; Linlin Zhang; Li Li; Wei Wang; Mingkun Liu; Guofan Zhang
Source:
Fish & shellfish immunology 2019 v.93 pp. 551-558
ISSN:
1050-4648
Subject:
Crassostrea gigas; amino acids; complementary DNA; enzymes; gene activation; gene expression; hemocytes; human cell lines; immune system; interferons; messenger RNA; mutants; open reading frames; oysters; phosphorylation; polyinosinic-polycytidylic acid; quantitative polymerase chain reaction; reporter genes; shellfish; signal transduction; transcription factor NF-kappa B; transfection
Abstract:
... IκB-related kinase ε (IKKε) plays a crucial role in the activation of nuclear factor κB (NF-κB) by phosphorylating inhibitor of NF-κB (IκB) and in the regulation of interferon (IFN) gene expression by phosphorylating IFN regulatory factors (IRFs). In this study, we cloned an IKKε homologue cDNA (designated as CgIKKε-like) from the Pacific oyster, Crassostrea gigas. The full 2896-bp cDNA sequence c ...
DOI:
10.1016/j.fsi.2019.07.074

24. Molecular characterization and functional analysis of a Rel gene in the Pacific oyster

Author:
Juan Dong; Xiuxiu Sang; Hongce Song; Rui Zhan; Lei Wei; Yaqiong Liu; Meiwei Zhang; Baoyu Huang; Xiaotong Wang
Source:
Fish & shellfish immunology 2020 v.101 pp. 9-18
ISSN:
1050-4648
Subject:
Crassostrea gigas; Pinctada fucata; amino acids; apoptosis; cell proliferation; dose response; gene overexpression; human cell lines; immune response; immune system; inflammation; interferons; interleukin-17; lipopolysaccharides; open reading frames; oysters; peptidoglycans; phylogeny; polyinosinic-polycytidylic acid; precipitin tests; reporter genes; sequence homology; signal transduction; tissues; transcription (genetics); transcription factor NF-kappa B; transfection; tumor necrosis factor-alpha
Abstract:
... The nuclear factor-κB (NF-κB) signaling pathway plays a crucial role in regulating many physiological processes such as development, inflammation, apoptosis, cell proliferation, differentiation and immune responses. And the NF-κB/Rel family members were considered as the most important transcription factors in the NF-κB signaling pathway. In this study, we cloned a Rel homolog gene (named as CgRel ...
DOI:
10.1016/j.fsi.2020.03.043

25. The involvement of a regucalcin in suppressing hemocyte apoptosis in Pacific oyster Crassostrea gigas

Author:
Xingye Lian; Shu Huang; Shuo Han; Qilin Yi; Weilin Wang; Jiejie Sun; Lingling Wang; Linsheng Song
Source:
Fish & shellfish immunology 2020 v.103 pp. 229-238
ISSN:
1050-4648
Subject:
Crassostrea gigas; amino acid sequences; amino acids; apoptosis; calcium; caspase-3; complementary DNA; enzyme activity; genome; gills; hemocytes; hepatopancreas; homeostasis; immune response; lipopolysaccharides; messenger RNA; nitric oxide; open reading frames; oysters; tissues
Abstract:
... Regucalcin (RGN), also known as senescence marker protein-30 (SMP30), plays a vital role in the regulation of Ca²⁺ homeostasis. In the present study, a regucalcin (designated as CgRGN) was identified from Pacific oyster Crassostrea gigas. The complete cDNA sequence of CgRGN was of 1059 bp, containing an open reading frame of 933 bp which encoded a protein of 310 amino acids. The deduced amino acid ...
DOI:
10.1016/j.fsi.2020.05.002

26. A new member of the runt domain family from Pacific oyster Crassostrea gigas (CgRunx) potentially involved in immune response and larvae hematopoiesis

Author:
Xiaorui Song; Ying Song; Miren Dong; Zhaoqun Liu; Weilin Wang; Lingling Wang; Linsheng Song
Source:
Fish & shellfish immunology 2019 v.89 pp. 228-236
ISSN:
1050-4648
Subject:
Crassostrea gigas; Vibrio splendidus; amino acids; cell nucleus; fluorescent antibody technique; gene expression regulation; gills; hematopoiesis; hemocytes; immune response; larvae; messenger RNA; oysters; polypeptides; shellfish; transcription factors
Abstract:
... The Runx family is a kind of heteromeric transcription factors, which is defined by the presence of a runt domain. As transcriptional regulator during development and cell fate specification, Runx is best known for its critical roles in hematopoiesis. In the present study, a Runx transcription factor (designed as CgRunx) was identified and characterized from the oyster Crassostrea gigas. The compl ...
DOI:
10.1016/j.fsi.2019.03.066

27. A novel tumor necrosis factor in the Pacific oyster Crassostrea gigas mediates the antibacterial response by triggering the synthesis of lysozyme and nitric oxide

Author:
Yan Zheng; Zhaoqun Liu; Lingling Wang; Meijia Li; Yukun Zhang; Yanan Zong; Yinan Li; Linsheng Song
Source:
Fish & shellfish immunology 2020 v.98 pp. 334-341
ISSN:
1050-4648
Subject:
Crassostrea gigas; Crassostrea hongkongensis; Mizuhopecten yessoensis; Vibrio splendidus; amino acid sequences; amino acids; antibacterial properties; apoptosis; blood serum; enzyme activity; epithelium; hemocytes; humans; lipopolysaccharides; lysozyme; messenger RNA; nitric oxide; open reading frames; oysters; peptidoglycans; phagocytosis; phylogeny; polypeptides; recombinant proteins; scallops; tumor necrosis factor-alpha
Abstract:
... Tumor necrosis factors (TNFs) are a group of multifunctional inflammatory cytokines involved in various pathological and immune processes. Recently, a few primitive TNFs have been characterized from molluscs, which play important roles in modulating cell apoptosis, phagocytosis and production of immune-related enzymes. In the present study, a novel TNF (named as CgTNF-2) with the activity to media ...
DOI:
10.1016/j.fsi.2019.12.073

28. CgAATase with specific expression pattern can be used as a potential surface marker for oyster granulocytes

Author:
Miren Dong; Xiaorui Song; Min Wang; Weilin Wang; Peng Zhang; Yu Liu; Meijia Li; Lingling Wang; Linsheng Song
Source:
Fish & shellfish immunology 2019 v.87 pp. 96-104
ISSN:
1050-4648
Subject:
Crassostrea gigas; Vibrio splendidus; alcohols; amino acids; density gradient centrifugation; fish; flow cytometry; ganglia; gene expression; gene expression regulation; granulocytes; hemocytes; hepatopancreas; immune response; in vitro culture; messenger RNA; monoclonal antibodies; muscles; open reading frames; oysters; peptides; pseudopodia; shellfish; transferases
Abstract:
... Granulocytes are known as the main immunocompetent hemocytes that play important roles in the immune defense of oyster Crassostrea gigas. In the present study, an alcohol acyltransferase (designed as CgAATase) with specific expression pattern was identified from oyster C. gigas, and it could be employed as a potential marker for the isolation of oyster granulocytes. The open reading frame (ORF) of ...
DOI:
10.1016/j.fsi.2019.01.003

29. CgNrdp1, a conserved negative regulating factor of MyD88-dependent Toll like receptor signaling in oyster Crassostrea gigas

Author:
Rui Sun; Limei Qiu; Qilin Yi; Mengqiang Wang; Feng Yue; Lingling Wang; Linsheng Song
Source:
Fish & shellfish immunology 2018 v.74 pp. 386-392
ISSN:
1050-4648
Subject:
Crassostrea gigas; amino acids; enzyme activity; enzyme-linked immunosorbent assay; fish; genome; immune response; luciferase; mammals; open reading frames; oysters; pathogens; polypeptides; shellfish; transcription factor NF-kappa B; ubiquitin-protein ligase
Abstract:
... Toll like receptor (TLR) signaling cascades are under precise regulations to ensure the proper immune responses during various pathogen invasions. The neuregulin receptor degradation protein-1 (Nrdp1) has been demonstrated to be a novel negative regulator of TLR signaling by targeting MyD88 to induce degradation in mammals. In the present study, an Nrdp1 homologue, CgNrdp1, was identified from the ...
DOI:
10.1016/j.fsi.2017.12.057

30. Characterisation and functional comparison of single-CRD and multidomain containing galectins CgGal-2 and CgGal-3 from oyster Crassostrea gigas

Author:
Mengmeng Huang; Tao Zhou; Yuehong Wu; Hui Fei; Gaoyang Wang; Zhi Li; Yutong Lei; Qian Liu; Cong Sun; Zhengbing Lv; Xue-Wei Xu
Source:
Fish & shellfish immunology 2018 v.78 pp. 238-247
ISSN:
1050-4648
Subject:
Crassostrea gigas; Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; Komagataella pastoris; Micrococcus luteus; Saccharomyces cerevisiae; Vibrio vulnificus; amino acid sequences; amino acids; bacterial infections; binding capacity; carbohydrate binding; coagulation; consensus sequence; erythrocytes; fungi; galactose; galectins; glucans; immune response; innate immunity; lipopolysaccharides; open reading frames; oysters; pathogen-associated molecular patterns; peptidoglycans; polypeptides; shellfish
Abstract:
... Galectins are β-galactoside binding lectins that play crucial roles in innate immunity in vertebrates and invertebrates through their conserved carbohydrate-recognition domains (CRDs). In the present study, single- and four-CRD-containing galectins were identified in oyster Crassostrea gigas (designated CgGal-2 and CgGal-3). The open reading frames (ORFs) of CgGal-2 and CgGal-3 encode polypeptides ...
DOI:
10.1016/j.fsi.2018.04.029

31. Gene cloning, expression, and X-ray crystallographic analysis of a β-mannanase from Eisenia fetida

Author:
Mitsuhiro Ueda; Yu Hirano; Hiroaki Fukuhara; Yuki Naka; Masami Nakazawa; Tatsuji Sakamoto; Yoshiyuki Ogata; Taro Tamada
Source:
Enzyme and microbial technology 2018 v.117 pp. 15-22
ISSN:
0141-0229
Subject:
Komagataella pastoris; Eisenia fetida; polyacrylamide gel electrophoresis; high performance liquid chromatography; genes; Daphnia pulex; Cryptopygus; amino acids; Crassostrea gigas; reaction mechanisms; Aplysia; molecular weight; amino acid sequences; hydrolysis; molecular cloning; temperature; glycosides; proteins; X-ray diffraction; glycosylation; beta-mannosidase; Mytilus edulis
Abstract:
... The endo-1,4-β-mannanases (Ef-Man) gene from Eisenia fetida was determined to consist of 1131 bp and encode a 377 amino acid protein. The amino acid sequence showed similarity with the endo-1,4-β-mannanases of Daphnia pulex (62%), Cryptopygus antarcticus (64%), Crassostrea gigas (61%), Mytilus edulis (60%), and Aplysia kurodai (58%). The gene encoding mature Ef-Man was expressed in Pichia pastoris ...
DOI:
10.1016/j.enzmictec.2018.05.014

32. A SPRY domain-containing SOCS box protein 3 (SPSB3) involved in the regulation of cytokine production in granulocytes of Crassostrea gigas

Author:
Min Wang; Chang Liu; Weilin Wang; Miren Dong; Peng Zhang; Yu Liu; Lingling Wang; Linsheng Song
Source:
Developmental and comparative immunology 2019 v.95 pp. 28-37
ISSN:
0145-305X
Subject:
Crassostrea gigas; RNA interference; Vibrio splendidus; amino acids; cell growth; cytokines; cytoplasm; fluorescent antibody technique; gene expression; genes; granulocytes; hemocytes; lipopolysaccharides; messenger RNA; open reading frames; oysters; polypeptides; tissues
Abstract:
... The sp1A/ryanodine receptor (SPRY) family members have been reported to involve in important biological pathways, including innate immune signaling, cytokine signaling suppression, development, cell growth, and retroviral restriction. In the present study, a SPRY domain-containing SOCS box protein (named as CgSPSB3) was identified and characterized from oyster Crassostrea gigas. The open reading f ...
DOI:
10.1016/j.dci.2019.01.013

33. A conserved interferon regulation factor 1 (IRF-1) from Pacific oyster Crassostrea gigas functioned as an activator of IFN pathway

Author:
Mengmeng Lu; Chuanyan Yang; Meijia Li; Qilin Yi; Guangxia Lu; Yichen Wu; Chen Qu; Lingling Wang; Linsheng Song
Source:
Fish & shellfish immunology 2018 v.76 pp. 68-77
ISSN:
1050-4648
Subject:
Crassostrea gigas; DNA-binding domains; amino acids; cytoplasm; enzyme activity; fish; gene expression; hemocytes; immune response; interferon regulatory factor-1; interferons; luciferase; messenger RNA; open reading frames; oysters; polypeptides; reporter genes; response elements; shellfish; tissues; transcription (genetics); transfection
Abstract:
... Interferon regulatory factors (IRFs), a family of transcription factors with a novel helix-turn-helix DNA-binding motif, play important roles in regulating the expression of interferons (IFNs) and IFN-stimulated genes. In the present study, an interferon regulation factor 1 was identified from oyster Crassostrea gigas (designated CgIRF-1), and its immune function was characterized to understand th ...
DOI:
10.1016/j.fsi.2018.02.024

34. The modulation of Smac/DIABLO on mitochondrial apoptosis induced by LPS in Crassostrea gigas

Author:
Zhao Lv; Xiaorui Song; Jiachao Xu; Zhihao Jia; Bin Yang; Yunke Jia; Limei Qiu; Lingling Wang; Linsheng Song
Source:
Fish & shellfish immunology 2019 v.84 pp. 587-598
ISSN:
1050-4648
Subject:
Crassostrea gigas; RNA interference; Western blotting; amino acids; apoptosis; caspase-3; defense mechanisms; fish; gene expression; hemocytes; immune response; lipopolysaccharides; membrane potential; mitochondria; mitochondrial membrane; necrosis; nucleotides; open reading frames; oysters; polypeptides; protein content; proteins; shellfish
Abstract:
... The mitochondrial pathway of apoptosis is well studied as the major mechanism of physiological cell death in vertebrates. In the present study, a second mitochondria-derived activator of caspases (Smac)/direct inhibitor of apoptosis-binding protein (IAP) with low pI protein (DIABLO) (designated as CgSmac) was identified from oyster Crassostrea gigas. The open reading frame of CgSmac was of 966 bp ...
DOI:
10.1016/j.fsi.2018.10.035

35. A novel caspase-associated recruitment domain (CARD) containing protein (CgCARDCP-1) involved in LPS recognition and NF-κB activation in oyster (Crassostrea gigas)

Author:
Feifei Wang; Zichao Yu; Weilin Wang; Yiqun Li; Guangxia Lu; Chen Qu; Hui Wang; Mengmeng Lu; Lingling Wang; Linsheng Song
Source:
Fish & shellfish immunology 2018 v.79 pp. 120-129
ISSN:
1050-4648
Subject:
Crassostrea gigas; Vibrio splendidus; amino acids; apoptosis; caspase-2; cytoplasm; fish; gene expression; gene expression regulation; genes; hemocytes; immune response; immunohistochemistry; inflammation; lipopolysaccharides; luciferase; messenger RNA; open reading frames; oysters; polypeptides; reverse transcriptase polymerase chain reaction; shellfish; signal transduction; tissues; transcription (genetics); transcription factor NF-kappa B
Abstract:
... Caspase-associated recruitment domain (CARD) containing proteins play critical roles in molecular interaction and regulation of various signaling pathways, such as the activation of caspase and NF-κB singling pathway in the process of apoptosis or inflammation. In the present study, a novel CARD containing protein (designed CgCARDCP-1) was identified and characterized from oyster Crassostrea gigas ...
DOI:
10.1016/j.fsi.2018.05.018

36. A novel globular C1q domain containing protein (C1qDC-7) from Crassostrea gigas acts as pattern recognition receptor with broad recognition spectrum

Author:
Yanan Zong; Zhaoqun Liu; Zhaojun Wu; Zirong Han; Lingling Wang; Linsheng Song
Source:
Fish & shellfish immunology 2019 v.84 pp. 920-926
ISSN:
1050-4648
Subject:
Aeromonas hydrophila; Crassostrea gigas; Crassostrea virginica; Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; Komagataella pastoris; Micrococcus luteus; Mizuhopecten yessoensis; Mytilus galloprovincialis; Staphylococcus aureus; Vibrio alginolyticus; Vibrio anguillarum; Vibrio splendidus; Yarrowia lipolytica; amino acids; binding capacity; fish; fungi; gills; hemocytes; hepatopancreas; immunology; ligands; lipopolysaccharides; messenger RNA; muscles; open reading frames; palps; pathogen-associated molecular patterns; peptidoglycans; phylogeny; polypeptides; receptors; recombinant proteins; shellfish; tissues
Abstract:
... The globular C1q domain containing (C1qDC) proteins are a family of versatile pattern recognition receptors (PRRs) to bind various ligands by their globular C1q (gC1q) domain. In the present study, a novel globular C1qDC (CgC1qDC-7) was characterized from Pacific oyster Crassostrea gigas. The open reading frame of CgC1qDC-7 was of 555 bp, encoding a polypeptide of 185 amino acids. Phylogenetic ana ...
DOI:
10.1016/j.fsi.2018.10.079

37. A single-CRD C-type lectin (CgCLec-3) with novel DIN motif exhibits versatile immune functions in Crassostrea gigas

Author:
Xiaorui Song; Xiaoyu Xin; Hao Wang; Hui Li; Huan Zhang; Zhihao Jia; Conghui Liu; Shuai Jiang; Lingling Wang; Linsheng Song
Source:
Fish & shellfish immunology 2019 v.92 pp. 772-781
ISSN:
1050-4648
Subject:
Crassostrea gigas; Escherichia coli; Komagataella pastoris; Micrococcus luteus; Staphylococcus aureus; Vibrio anguillarum; Vibrio splendidus; Yarrowia lipolytica; amino acid sequences; amino acids; antibacterial properties; bacteria; binding capacity; hemocytes; hepatopancreas; immune response; immunohistochemistry; lectins; lipopolysaccharides; mannose; muscles; oysters; pathogen-associated molecular patterns; pathogens; peptidoglycans; phagocytosis; receptors; recombinant proteins; shellfish
Abstract:
... C-type lectins (CTLs), as important pattern recognition receptors (PRRs), are a superfamily of Ca2+-dependent carbohydrate-recognition proteins which participate in nonself-recognition and eliminating pathogens. In the present study, a novel CTL (designated as CgCLec-3) was identified from the Pacific oyster Crassostrea gigas. There was only one carbohydrate-recognition domain (CRD) of 151 amino a ...
DOI:
10.1016/j.fsi.2019.07.001

38. A vital ubiquitin-conjugating enzyme CgUbe2g1 participated in regulation of immune response of Pacific oyster Crassostrea gigas

Author:
Rui Liu; Qi Cheng; Xiaorui Song; Hao Wang; Xiudan Wang; Lingling Wang; Beiwei Zhu; Linsheng Song
Source:
Developmental and comparative immunology 2019 v.91 pp. 132-142
ISSN:
0145-305X
Subject:
Crassostrea gigas; amino acids; apoptosis; cell communication; cell-mediated immunity; hemocytes; hepatopancreas; immune response; inflammation; innate immunity; lipopolysaccharides; males; messenger RNA; molecular weight; oysters; pathogens; phylogeny; polypeptides; transcription factor NF-kappa B; tumor necrosis factor-alpha; ubiquitin; ubiquitin-protein ligase; ubiquitination; vertebrates
Abstract:
... As an important post-translational protein modification, ubiquitination has been demonstrated to play a vital role in immune response of vertebrates. Ubiquitin (Ub)-conjugating enzyme E2 is the “heart” of ubiquitination, which is responsible for Ub cellular signaling and substrate modification. In the present study, an Ub-conjugating enzyme E2 (designed as CgUbe2g1) was identified from oyster Cras ...
DOI:
10.1016/j.dci.2018.10.014

39. Antimicrobial effect of the 60S ribosomal protein L29 (cgRPL29), purified from the gill of pacific oyster, Crassostrea gigas

Author:
Jung-Kil Seo; Dong-Gyun Kim; Ryunkyoung Oh; Kwon-Sam Park; In-Ah Lee; Sang-Man Cho; Ki–Young Lee; Bo-Hye Nam
Source:
Fish & shellfish immunology 2017 v.67 pp. 675-683
ISSN:
1050-4648
Subject:
5' untranslated regions; Crassostrea gigas; amino acid sequences; amino acids; antimicrobial peptides; antimicrobial properties; aquatic invertebrates; cation exchange; complementary DNA; gene expression; models; molecular weight; open reading frames; prediction; reversed-phase high performance liquid chromatography; ribosomal proteins; tissue distribution
Abstract:
... We purified an ∼6.4-kDa antimicrobial peptide from an acidified gill extract of the Pacific oyster, Crassostrea gigas, by cation-exchange and C18 reversed-phase high performance liquid chromatography (HPLC). The identified peptide was composed of 54 amino acids and had a molecular weight of 6484.6 Da. Comparison of the amino acid sequence and molecular weight with those of other known proteins or ...
DOI:
10.1016/j.fsi.2017.06.058

40. A DM9-containing protein from oyster Crassostrea gigas (CgDM9CP-2) serves as a multipotent pattern recognition receptor

Author:
Yu Liu; Peng Zhang; Weilin Wang; Miren Dong; Min Wang; Changhao Gong; Zhihao Jia; Zhaoqun Liu; Anguo Zhang; Lingling Wang; Linsheng Song
Source:
Developmental and comparative immunology 2018 v.84 pp. 315-326
ISSN:
0145-305X
Subject:
Staphylococcus aureus; lipopolysaccharides; Komagataella pastoris; phylogeny; genes; Gram-positive bacteria; polypeptides; Vibrio splendidus; Micrococcus luteus; open reading frames; Yarrowia lipolytica; Vibrio anguillarum; gene expression; amino acids; Crassostrea gigas; hepatopancreas; binding capacity; Drosophila melanogaster; tissues; Escherichia coli; Gram-negative bacteria; messenger RNA; Aeromonas hydrophila; muscles; mannose; oysters; protein domains; innate immunity; lectins; peptidoglycans; gills; hemocytes; fungi
Abstract:
... DM9 is a novel protein domain with unknown function originally discovered in Drosophila melanogaster. Recently, a protein harboring DM9 repeats was identified as mannose-specific lectin (CgCGL1, renamed as CgDM9CP-1) from the Pacific oyster Crassostrea gigas. In the present study, another DM9 containing protein was identified from oyster C. gigas (designated as CgDM9CP-2). The open reading frame o ...
DOI:
10.1016/j.dci.2018.03.003

41. Molecular characterization and expression profiles of myosin essential light chain gene in the Pacific oyster Crassostrea gigas

Author:
Hong Yu; Huijuan Li; Qi Li
Source:
Comparative biochemistry and physiology 2017 v.213 pp. 1-7
ISSN:
1096-4959
Subject:
Crassostrea gigas; amino acid sequences; amino acids; binding sites; calcium; complementary DNA; genes; in situ hybridization; larvae; messenger RNA; muscle contraction; muscles; myosin; open reading frames; oysters; quantitative analysis; sequence analysis
Abstract:
... In molluscs, muscle contraction is triggered by a direct binding of Ca²⁺ to myosin. The myosin essential light chain (MELC) provides the Ca²⁺ binding site and is of importance for motor function and regulation. In this study, the complete cDNA sequence of MELC gene of the Pacific oyster, Crassostrea gigas, was obtained, and the expression profiles were performed in different tissues and different ...
DOI:
10.1016/j.cbpb.2017.07.007

42. A cysteine protease (cathepsin Z) from disk abalone, Haliotis discus discus: Genomic characterization and transcriptional profiling during bacterial infections

Author:
G.I. Godahewa; N.C.N. Perera; Sukkyoung Lee; Myoung-Jin Kim; Jehee Lee
Source:
Gene 2017 v.627 pp. 500-507
ISSN:
0378-1119
Subject:
Crassostrea gigas; Haliotis discus; Listeria monocytogenes; Vibrio parahaemolyticus; abalone; active sites; acute phase proteins; amino acids; bacteria; bacterial infections; cathepsin X; dendritic cells; developmental stages; early development; exons; glycosylation; hemocytes; immune response; introns; lipopolysaccharides; macrophages; molecular weight; monocytes; papain; phagocytosis; phylogeny; quantitative polymerase chain reaction; signal peptide; signal transduction; tissues; transcription (genetics); vertebrates
Abstract:
... Cathepsin Z (CTSZ) is lysosomal cysteine protease of the papain superfamily. It participates in the host immune defense via phagocytosis, signal transduction, cell-cell communication, proliferation, and migration of immune cells such as monocytes, macrophages, and dendritic cells. Hence, CTSZ is also acknowledged as an acute-phase protein in host immunity. In this study, we sought to identify the ...
DOI:
10.1016/j.gene.2017.07.007

43. A molluscan extracellular signal-regulated kinase is involved in host response to immune challenges in vivo and in vitro

Author:
Fufa Qu; Zhiming Xiang; Jun Li; Shu Xiao; Fan Mao; Yanping Qin; Yingli Zhou; Haitao Ma; Ziniu Yu
Source:
Fish & shellfish immunology 2017 v.62 pp. 311-319
ISSN:
1050-4648
Subject:
Crassostrea gigas; Staphylococcus haemolyticus; Vibrio alginolyticus; amino acids; binding sites; cytokines; cytoplasm; embryogenesis; immune response; inflammation; lipopolysaccharides; mitogen-activated protein kinase; molecular weight; open reading frames; oysters; pathogens; peptidoglycans; phosphorylation; phylogeny; polypeptides; quantitative polymerase chain reaction; tissues; China
Abstract:
... Extracellular signal-regulated kinases (ERKs) are a group of highly conserved serine/threonine-specific protein kinases that function as important signaling intermediates in mitogen-activated protein kinase (MAPK) pathways, which are involved in a wide variety of cellular activities, including proliferation, inflammation and cytokine production. However, little is known about the roles of this kin ...
DOI:
10.1016/j.fsi.2017.01.038

44. A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas

Author:
Yunke Jia; Bin Yang; Wenjing Dong; Zhaoqun Liu; Zhao Lv; Zhihao Jia; Limei Qiu; Lingling Wang; Linsheng Song
Source:
Developmental and comparative immunology 2018 v.82 pp. 83-93
ISSN:
0145-305X
Subject:
Crassostrea gigas; amino acids; antagonists; apoptosis; blood serum; calcium; cyclic AMP; gene expression; hemocytes; hepatopancreas; humoral immunity; immune response; immunomodulation; ion channels; lipopolysaccharides; messenger RNA; open reading frames; oysters; polypeptides; serotonin; serotonin receptors; superoxide dismutase; tissues; transfection; tumor necrosis factors
Abstract:
... Serotonin receptors, including ligand-gated ion channel (LGICs) and G protein-coupled receptors (GPCR), play vital roles in modulating physiological processes and immunoreaction. In the present study, a homologue of serotonin (5-HT) receptor was identified from oyster Crassostrea gigas (designated Cg5-HTR-1). Its open reading frame (ORF) was of 1239 bp, encoding a polypeptide of 412 amino acids wi ...
DOI:
10.1016/j.dci.2017.12.029

45. A shell-formation related carbonic anhydrase in Crassostrea gigas modulates intracellular calcium against CO2 exposure: Implication for impacts of ocean acidification on mollusk calcification

Author:
Xiudan Wang; Mengqiang Wang; Zhihao Jia; Xiaorui Song; Lingling Wang; Linsheng Song
Source:
Aquatic toxicology 2017 v.189 pp. 216-228
ISSN:
0166-445X
Subject:
Crassostrea gigas; RNA interference; acetazolamide; amino acids; bicarbonates; biomineralization; calcification; calcium; carbon dioxide; carbon dioxide enrichment; carbonate dehydratase; complementary DNA; early development; gene expression; genes; hemocytes; hepatopancreas; ions; larvae; messenger RNA; molluscs; muscles; ocean acidification; polypeptides; signal peptide; tissues; vertebrates
Abstract:
... Ocean acidification (OA) could decrease the shells and skeletons formation of mollusk by reducing the availability of carbonate ions at calcification sites. Carbonic anhydrases (CAs) convert CO2 to HCO3− and play important roles in biomineralization process from invertebrate to vertebrate. In the present study, a CA (designated as CgCA) was identified and characterized in Pacific oyster C. gigas. ...
DOI:
10.1016/j.aquatox.2017.06.009

46. Characterization and expression of a novel caspase gene: Evidence of the expansion of caspases in Crassostrea gigas

Author:
Zhongxiao Li; Chao Wang; Fengjuan Jiang; Pin Huan; Baozhong Liu
Source:
Comparative biochemistry and physiology 2016 v.201 pp. 37-45
ISSN:
1096-4959
Subject:
Crassostrea gigas; adults; amino acid sequences; amino acids; apoptosis; caspases; gene expression; genes; gills; hepatopancreas; messenger RNA; molecular cloning; oocytes; open reading frames; oysters; proteins; seawater; thermal stress
Abstract:
... Caspases are a group of cysteine-aspartate proteases involved in apoptosis and a variety of non-apoptotic processes. In this study, a novel caspase gene was cloned and its potential role in apoptosis was investigated. The caspase gene (CgCasp 3/7) has an open reading frame of 1626bp encoding 541 amino acids containing the conserved functional domains and motifs of effector caspases. Its amino acid ...
DOI:
10.1016/j.cbpb.2016.07.001

47. Cloning and differential expression of a novel toll-like receptor gene in noble scallop Chlamys nobilis with different total carotenoid content

Author:
Yeqing Lu; Huaiping Zheng; Hongkuan Zhang; Jianqin Yang; Qiang Wang
Source:
Fish & shellfish immunology 2016 v.56 pp. 229-238
ISSN:
1050-4648
Subject:
Chlamys nobilis; Crassostrea gigas; Mytilus galloprovincialis; Toll-like receptors; Vibrio parahaemolyticus; amino acids; carotenoids; complementary DNA; gene expression; gene expression regulation; genes; hemocytes; hepatopancreas; immune response; intestines; kidneys; lipopolysaccharides; open reading frames; pathogens; phylogeny; scallops
Abstract:
... To investigate whether toll like receptors (TLRs) genes do have an immune influence on noble scallop Chlamys nobilis under pathogen stress, acute challenges lasting 48 h to Vibrio parahaemolyticus, lipopolysaccharide (LPS), polyinosinic polycytidylic acid (Poly I:C), and PBS were conducted in two scallop stains of orange and brown with different carotenoids content. A novel toll-like receptor gene ...
DOI:
10.1016/j.fsi.2016.07.007

48. Comparative study of two single CRD C-type lectins, CgCLec-4 and CgCLec-5, from pacific oyster Crassostrea gigas

Author:
Zhihao Jia; Huan Zhang; Shuai Jiang; Mengqiang Wang; Lingling Wang; Linsheng Song
Source:
Fish & shellfish immunology 2016 v.59 pp. 220-232
ISSN:
1050-4648
Subject:
Crassostrea gigas; Escherichia coli; Staphylococcus aureus; Yarrowia lipolytica; amino acids; antimicrobial properties; bacteria; binding capacity; binding sites; calcium; disulfide bonds; fish; fungi; hemocytes; hepatopancreas; immune response; innate immunity; lectins; lipopolysaccharides; open reading frames; oysters; pathogens; polypeptides; recombinant proteins; shellfish; signal peptide
Abstract:
... C-type lectins (CTLs), a superfamily of Ca2+-dependent carbohydrate-recognition proteins, are involved in nonself-recognition and pathogen elimination, and play crucial roles in the innate immunity. In the present study, two single CRD C-type lectins, CgCLec-4 and CgCLec-5, were identified from oyster Crassostrea gigas. The open reading frame (ORF) of CgCLec-4 and CgCLec-5 encoded polypeptides of ...
DOI:
10.1016/j.fsi.2016.10.030

49. Gene Co-Expression Network Analysis Reveals the Correlation Patterns Among Genes in Euryhaline Adaptation of Crassostrea gigas

Author:
Xuelin Zhao; Hong Yu; Lingfeng Kong; Qi Li
Source:
Marine biotechnology 2016 v.18 no.5 pp. 535-544
ISSN:
1436-2228
Subject:
Crassostrea gigas; amino acids; aquaculture; biosynthesis; electron transfer; euryhaline species; free amino acids; gene regulatory networks; genes; gills; osmotic stress; oxidation; oysters; salinity; salt stress; salt tolerance; sequence analysis; transcriptome; Atlantic Ocean
Abstract:
... The Pacific oyster Crassostrea gigas is a dominant aquaculture species in many intertidal zones throughout the Pacific and Atlantic Oceans and can tolerate a wide range of salinity. Studying the gene expression profiles of oyster gills had found differentially expressed genes (DEGs) involved in salinity tolerance. A systematic study of cellular response to salinity stress may provide insights into ...
DOI:
10.1007/s10126-016-9715-7

50. The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through TNF pathway in oyster Crassostrea gigas

Author:
Zhaoqun Liu; Zhi Zhou; Lingling Wang; Wenjing Dong; Limei Qiu; Linsheng Song
Source:
Developmental and comparative immunology 2016 v.65 pp. 139-148
ISSN:
0145-305X
Subject:
Acromyrmex echinatior; Crassostrea gigas; Echinococcus granulosus; Habropoda laboriosa; acetylcholine; amino acids; antagonists; apoptosis; calcium; cholinergic receptors; cyclic AMP; gene expression; hemocytes; ligands; messenger RNA; muscles; open reading frames; oysters; phylogeny; transfection; tumor necrosis factors
Abstract:
... Muscarinic receptors, which selectively take muscarine as their ligand, are critical for the immunological and physiological processes in animals. In the present study, the open region frame (ORF) of a homologue of muscarinic acetylcholine (ACh) receptor (mAChR) was amplified from oyster Crassostrea gigas (named as CgmAChR-1), whose full length was 1983 bp and the protein it encoded contained 660  ...
DOI:
10.1016/j.dci.2016.07.003