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You searched for: Subject term amino acids Remove constraint Subject term: amino acids Subject term beta-glucosidase Remove constraint Subject term: beta-glucosidase
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1. Effect of Azamethiphos on enzymatic activity and metabolic fingerprints of marine microbial communities from the water column

Author:
Diana V. Garcés; Marcelo E. Fuentes; Renato A. Quiñones
Source:
Aquaculture 2020 v.529 pp. 735650
ISSN:
0044-8486
Subject:
Caligus rogercresseyi; aerobiosis; amino acids; azamethiphos; bacteria; bacterioplankton; beta-glucosidase; carbohydrate metabolism; carbon; carboxylic acids; enzyme activity; financial economics; fish culture; fish farms; fish production; malate dehydrogenase; marine fungi; microbial communities; nontarget organisms; Chile
Abstract:
... Salmon production in Chile has increased significantly since the 1990s. However, there have been significant economic losses owing to outbreaks of Caligus rogercresseyi. Pesticides, among them Azamethiphos, are used to mitigate the impact of this sea louse. Azamethiphos can affect non-target species living near salmon farms such as microbial plankton, although there is little information about thi ...
DOI:
10.1016/j.aquaculture.2020.735650

2. Analysis of chickpea gene co-expression networks and pathways during heavy metal stress

Author:
Birendra Singh Yadav; Swati Singh; Sameer Srivastava; Ashutosh Mani
Source:
Journal of biosciences 2019 v.44 no.4 pp. 99
ISSN:
0250-5991
Subject:
F-box proteins; abiotic stress; amino acids; arsenic; beta-glucosidase; biochemical pathways; biosynthesis; biotic stress; cadmium; carboxylic ester hydrolases; cellulose synthase; chickpeas; chromium; gene expression regulation; genes; heavy metals; indole alkaloids; isoflavones; microarray technology; riboflavin; stress tolerance
Abstract:
... Crop productivity and yield are adversely affected by abiotic and biotic stresses. Therefore, finding out the genes responsible for stress tolerance is a significant stride towards crop improvement. A gene co-expression network is a powerful tool to detect the most connected genes during heavy metal (HM) stress in plants. The most connected genes may be responsible for HM tolerance by altering the ...
DOI:
10.1007/s12038-019-9899-x

3. Bioprocessed Production of Resveratrol-Enriched Rice Wine: Simultaneous Rice Wine Fermentation, Extraction, and Transformation of Piceid to Resveratrol from Polygonum cuspidatum Roots

Author:
Kai-Ruei Yang; Hui-Chuan Yu; Chun-Yung Huang; Jen-Min Kuo; Cheng Chang; Chwen-Jen Shieh; Chia-Hung Kuo
Source:
Foods 2019 v.8 no.7 pp. -
ISSN:
2304-8158
Subject:
2,2-diphenyl-1-picrylhydrazyl; Reynoutria japonica; acidity; amino acids; antioxidant activity; beta-glucosidase; bioprocessing; crop production; ethanol; fermentation; heat treatment; iron; pH; plate count; reducing sugars; refrigeration; resveratrol; rice wines; roots; shelf life; temperature; ultrafiltration
Abstract:
... A new bioprocess to produce resveratrol-enriched rice wine was established and the effects of adding Polygonum cuspidatum root powder to rice wine fermentation were investigated. In this new process, piceid and resveratrol were extracted from P. cuspidatum roots to rice wine and piceid was converted to resveratrol by β-glucosidase during fermentation. After 10 days co-fermentation, rice wine ...
DOI:
10.3390/foods8070258
PubMed:
31311091
PubMed Central:
PMC6678416

4. Effects of ultrasound irradiation on the properties of apricot kernels during accelerated debitterizing

Author:
Qing-An Zhang; Fang-Fang Shi; Jian-Li Yao; Ning Zhang
Source:
RSC advances 2020 v.10 no.18 pp. 10624-10633
ISSN:
2046-2069
Subject:
amino acids; apricots; beta-glucosidase; circular dichroism spectroscopy; color; electrophoresis; fluorescence; fluorescence emission spectroscopy; lipid content; oils; seeds; texture; thermal properties; ultrasonic treatment; ultrasonics; ultraviolet-visible spectroscopy
Abstract:
... In this paper, studies were conducted to investigate the effects of ultrasonically accelerated debitterizing on the physicochemical properties of apricot kernels, such as color, texture, oil content, protein characteristics and amino acids, with UV-Vis spectroscopy, synchronous fluorescence spectroscopy, circular dichroism, electrophoresis, environmental scanning electron microscopy, and thermal p ...
DOI:
10.1039/c9ra10965j

5. Farmyard manure applications stimulate soil carbon and nitrogen cycling by boosting microbial biomass rather than changing its community composition

Author:
Qingxu Ma; Yuan Wen; Deying Wang; Xiaodan Sun; Paul W. Hill; Andy Macdonald; David R. Chadwick; Lianghuan Wu; Davey L. Jones
Source:
Soil biology & biochemistry 2020 v.144 pp. 107760
ISSN:
0038-0717
Subject:
NPK fertilizers; amino acids; ammonium; animal manures; beta-glucosidase; carbon dioxide; carbon dioxide production; cellulose; cellulose 1,4-beta-cellobiosidase; community structure; enzyme activity; extracellular enzymes; fertilizer application; field experimentation; glucose; half life; isotope labeling; land application; leucyl aminopeptidase; long term effects; manure spreading; microbial biomass; microbial carbon; microbial communities; nitrates; nitrogen; nitrogen cycle; peptides; phospholipid fatty acids; proteinases; proteins; soil carbon; soil fertility; soil microorganisms; soil organic nitrogen; soil texture; subsoil; topsoil
Abstract:
... Land application of farmyard manure (FYM) is a widespread agronomic practice used to enhance soil fertility, but its long-term effects on soil microbial carbon (C) and nitrogen (N) cycling have not been investigated in detail. Topsoils (0–23 cm) and subsoils (23–38 cm) were collected from a field trial on a sandy-textured soil where FYM had been applied at high (50–25 t ha⁻¹ yr⁻¹, 28 yr) and low r ...
DOI:
10.1016/j.soilbio.2020.107760

6. Preliminary Characterization of Yeasts from Bombino Bianco, a Grape Variety of Apulian Region, and Selection of an Isolate as a Potential Starter

Author:
Barbara Speranza; Daniela Campaniello; Leonardo Petruzzi; Milena Sinigaglia; Maria Rosaria Corbo; Antonio Bevilacqua
Source:
Fermentation 2019 v.5 no.4 pp. -
ISSN:
2311-5637
Subject:
Saccharomyces cerevisiae; acetic acid; amino acids; beta-glucosidase; ethanol; fermentation; grapes; hydrolysis; pectins; sulfites; sulfur dioxide; yeasts; Italy
Abstract:
... Eighty-seven yeasts were isolated from Bombino bianco, a white grape variety from Apulian Region (Southern Italy). The isolates were characterized for the splitting of arbutin, the hydrolysis of pectins, sulphite production, the resistance to acetic acid, SO₂, and ethanol. An enhanced arbutin splitting (β-glucosidase) and a moderate pectolytic activity were found. Concerning ethanol resistance, th ...
DOI:
10.3390/fermentation5040102

7. Rational molecular design for improving digestive enzyme resistance of beta-glucosidase from Trichoderma viride based on inhibition of bound state formation

Author:
Hao Wang; Xiangna Lin; Shuang Li; Jianlin Lin; Chunfang Xie; Daling Liu; Dongsheng Yao
Source:
Enzyme and microbial technology 2020 v.133 pp. 109465
ISSN:
0141-0229
Subject:
Komagataella pastoris; Trichoderma viride; amino acids; beta-glucosidase; feed industry; feeds; half life; intestines; livestock farming; mutants; pepsin; prediction; trypsin
Abstract:
... Beta-glucosidase (BGL1) is widely used in animal feed industries. However, degradation caused by digestive enzymes in the intestine hampers its application. Improving the resistance of feed enzymes against proteases is crucial in livestock farming. To improve the resistance of beta-glucosidase against pepsin and trypsin, a rational molecular design based on the inhibition of bound-state formation ...
DOI:
10.1016/j.enzmictec.2019.109465

8. Tyr320 is a molecular determinant of the catalytic activity of β-glucosidase from Neosartorya fischeri

Author:
Ramasamy Shanmugam; In-Won Kim; Manish K. Tiwari; Hui Gao; Primata Mardina; Devashish Das; Anurag Kumar; Marimuthu Jeya; Sang-Yong Kim; Young Sin Kim; Jung-Kul Lee
Source:
International journal of biological macromolecules 2020 v.151 pp. 609-617
ISSN:
0141-8130
Subject:
Aspergillus fischeri; amino acids; aromatic acids; beta-glucosidase; biomass; catalytic activity; endo-1,4-beta-glucanase; enzyme activity; enzyme substrates; hydrophilicity; lignocellulose; mechanism of action; models; mutants; screening; sequence alignment; site-directed mutagenesis
Abstract:
... β-Glucosidases (BGL) are key members of the cellulase enzyme complex that determine efficiency of lignocellulosic biomass degradation, which have shown great functional importance to many biotechnological systems. A previous reported BGL from Neosartorya fischeri (NfBGL) showed much higher activity than other BGLs. Screening the important residues based on sequence alignment, analyzing a homology ...
DOI:
10.1016/j.ijbiomac.2020.02.117

9. Cloning, purification, and characterization of the organic solvent tolerant β-glucosidase, OaBGL84, from Olleya aquimaris DAU311

Author:
Eun-Jung Hwang; Yong-Suk Lee; Yong-Lark Choi
Source:
Han'guk Ŭngyong Saengmyŏng Hwahakhoe chi = 2018 v.61 no.3 pp. 325-336
ISSN:
1738-2203
Subject:
amino acid sequences; amino acids; aquatic bacteria; benzene; beta-glucosidase; cellulose; copper; enzyme activity; genes; glycosides; hexane; ions; isoflavones; mercury; molecular cloning; open reading frames; pH; polyacrylamide gel electrophoresis; sequence analysis; solvents; temperature; toluene; South Korea
Abstract:
... A marine bacterium, Olleya aquimaris DAU311, was isolated from Goraebul beach in the Republic of Korea. This strain had β-glucosidase activity on Luria–Bertani esculin plates. The β-glucosidase, oabgl84, was isolated, cloned, and sequenced, based on fosmid library. The gene encoded novel β-glucosidase and consisted of an open reading frame of 2304 bp, which encodes 768 amino acids. The deduced ami ...
DOI:
10.1007/s13765-018-0361-9

10. Cyanogenesis in Arthropods: From Chemical Warfare to Nuptial Gifts

Author:
Mika Zagrobelny; Érika Cristina Pinheiro de Castro; Birger Lindberg Møller; Søren Bak
Source:
Insects 2018 v.9 no.2 pp. -
ISSN:
2075-4450
Subject:
Chilopoda; Coleoptera; Diplopoda; aldehydes; amino acids; beta-glucosidase; biochemical pathways; biosynthesis; butterflies; catalytic activity; chemical defenses; convergent evolution; cyanogenesis; cytochrome P-450; electrons; energy; excretion; food plants; glucosides; hydrogen cyanide; hydrolysis; ingestion; ketones; lyases; manufacturing; mites; moths; nitrogen metabolism; phylogeny; predators; thiosulfate sulfurtransferase; toxicity
Abstract:
... Chemical defences are key components in insect–plant interactions, as insects continuously learn to overcome plant defence systems by, e.g., detoxification, excretion or sequestration. Cyanogenic glucosides are natural products widespread in the plant kingdom, and also known to be present in arthropods. They are stabilised by a glucoside linkage, which is hydrolysed by the action of β-g ...
DOI:
10.3390/insects9020051
PubMed:
29751568
PubMed Central:
PMC6023451

11. Description of Alteromonas abrolhosensis sp. nov., isolated from sea water of Abrolhos Bank, Brazil

Author:
Maria S. Nóbrega; Bruno S. Silva; Luciana Leomil; Diogo Antonio Tschoeke; Mariana E. Campeão; Gizele D. Garcia; Graciela A. Dias; Verônica V. Vieira; Cristiane C. Thompson; Fabiano L. Thompson
Source:
Antonie van Leeuwenhoek 2018 v.111 no.7 pp. 1131-1138
ISSN:
0003-6072
Subject:
Alteromonas macleodii; DNA; acetates; aerobes; amino acids; bacteria; beta-glucosidase; carboxylic ester hydrolases; enzyme activity; galactose; glycerol; maltose; mannitol; new species; nucleic acid hybridization; phenotype; ribosomal RNA; seawater; sequence analysis; xylose; Brazil
Abstract:
... Two Gram-negative, motile, aerobic bacteria isolated from waters of the Abrolhos Bank were classified through a whole genome-based taxonomy. Strains PEL67Eᵀ and PEL68C shared 99% 16S rRNA and dnaK sequence identity with Alteromonas marina SW-47ᵀ and Alteromonas macleodii ATCC 27126ᵀ. In silico DNA–DNA Hybridization, i.e. genome-to-genome distance (GGD), average amino acid identity (AAI) and averag ...
DOI:
10.1007/s10482-018-1016-x

12. Diversity and function of soil bacterial communities in response to long-term intensive management in a subtropical bamboo forest

Author:
Junhui Chen; Qifeng Wu; Songhao Li; Jiangfei Ge; Chenfei Liang; Hua Qin; Qiufang Xu; Jeffry J. Fuhrmann
Source:
Geoderma 2019 pp. 113894
ISSN:
0016-7061
Subject:
Phyllostachys edulis; amino acids; bacterial communities; bamboos; beta-glucosidase; biogeochemical cycles; carbohydrates; chronosequences; community structure; enzyme activity; forest management; forests; functional diversity; intensive forestry; microbiome; nitrate nitrogen; nutrient availability; nutrient content; phosphorus; phylogeny; plant growth; soil bacteria; soil enzymes; soil pH; soil structure; structural equation modeling; understory; China
Abstract:
... Intensive forest management practices, such as fertilization, understory removal and deep tilling, play an important role in improving plant growth in forests through altering nutrient availability and soil structure. However, how such management affects soil microbial community diversity and functions related to nutrient cycling remains largely unknown. In this study, we investigated the response ...
DOI:
10.1016/j.geoderma.2019.113894

13. Draft genome analysis of lignocellulolytic enzymes producing Aspergillus terreus with structural insight of β-glucosidases through molecular docking approach

Author:
Tripti Dadheech; Subhash Jakhesara; Prakram Singh Chauhan; Ramesh Pandit; Ankit Hinsu; Anju Kunjadiya; Dharamshibhai Rank; Chaitanya Joshi
Source:
International journal of biological macromolecules 2019 v.125 pp. 181-190
ISSN:
0141-8130
Subject:
Aspergillus terreus; amino acids; beta-glucosidase; catalytic activity; cellobiose; computer simulation; genes; genome assembly; hydrogen bonding; hydrolysis; lignocellulases; models; polysaccharides; rumen; value added
Abstract:
... Members of the genus Aspergillus are extensively studied ascomycetes because of their ability to synthesize high value-added compounds and enzymes of industrial interest. Precise whole genome assembly and gene annotation are significant for gene functional analyses. Here, we report the draft genome sequencing, assembly and whole genome analysis of Aspergillus terreus P14_T3, isolated from rumen sa ...
DOI:
10.1016/j.ijbiomac.2018.12.020

14. Identification of novel glycoside hydrolases via whole genome sequencing of Niabella ginsenosidivorans for production of various minor ginsenosides

Author:
Muhammad Zubair Siddiqi; Majid S. Hashmi; Jung-Mi Oh; Sungkun Chun; Wan-Taek Im
Source:
3 Biotech 2019 v.9 no.7 pp. 258
ISSN:
2190-572X
Subject:
Escherichia coli; alpha-L-fucosidase; alpha-N-arabinofuranosidase; alpha-galactosidase; amino acid sequences; amino acids; beta-glucosidase; clones; genes; ginsenosides; molecular weight; polyacrylamide gel electrophoresis; proteins; sequence analysis; trehalase
Abstract:
... In this study, many bacterial strains were screened for the production of minor ginsenosides, but based on conversion competence among the strains, the strain Niabella ginsenosidivorans BS26ᵀ has the good ginsenoside-transforming ability. Therefore, the strain BS26ᵀ was selected for complete genome sequence analysis to determine the target (glycoside hydrolase) functional genes. Whole genome analy ...
DOI:
10.1007/s13205-019-1776-7
PubMed:
31192083
PubMed Central:
PMC6556516

15. Molecular Characterization and Potential Synthetic Applications of GH1 β-Glucosidase from Higher Termite Microcerotermes annandalei

Author:
Siriphan Arthornthurasuk; Wantha Jenkhetkan; Eukote Suwan; Daranee Chokchaichamnankit; Chantragan Srisomsap; Pakorn Wattana-Amorn; Jisnuson Svasti; Prachumporn T. Kongsaeree
Source:
Applied biochemistry and biotechnology 2018 v.186 no.4 pp. 877-894
ISSN:
0273-2289
Subject:
Microcerotermes; amino acid sequences; amino acids; beta-glucosidase; biosynthesis; biotechnology; biotransformation; functional properties; glucose; glucosides; glycosylation; hydrolysis; intracellular enzymes; molecular weight; screening; secretion
Abstract:
... A novel β-glucosidase from higher termite Microcerotermes annandalei (MaBG) was obtained via a screening method targeting β-glucosidases with increased activities in the presence of glucose. The purified natural MaBG showed a subunit molecular weight of 55 kDa and existed in a native form as a dimer without any glycosylation. Gene-specific primers designed from its partial amino acid sequences wer ...
DOI:
10.1007/s12010-018-2781-8

16. Nanoimmobilization of β-glucosidase onto hydroxyapatite

Author:
Thamara C. Coutinho; Mayerlenis J. Rojas; Paulo W. Tardioli; Elaine C. Paris; Cristiane S. Farinas
Source:
International journal of biological macromolecules 2018 v.119 pp. 1042-1051
ISSN:
0141-8130
Subject:
Fourier transform infrared spectroscopy; adsorption; amino acids; beta-glucosidase; beverages; biorefining; calcium; catalysts; desorption; enzymatic hydrolysis; hydroxyapatite; immobilized enzymes; ionic strength; nanoparticles; pH; protocols; zeta potential
Abstract:
... β-Glucosidase is an enzyme of great industrial interest that is used in biorefineries and in the pharmaceutical, food, and beverage sectors, among others. These industrial processes would benefit from the use of immobilized enzyme systems that allow several reuses of the enzyme. A promising inorganic and nontoxic material for such application is hydroxyapatite (HA), which can be synthesized at nan ...
DOI:
10.1016/j.ijbiomac.2018.08.042

17. Biosurfactant–Protein Interaction: Influences of Mannosylerythritol Lipids-A on β-Glucosidase

Author:
Linlin Fan; Pujun Xie; Ying Wang; Zisu Huang; Jianzhong Zhou
Source:
Journal of agricultural and food chemistry 2018 v.66 no.1 pp. 238-246
ISSN:
1520-5118
Subject:
amino acids; beta-glucosidase; biosurfactants; circular dichroism spectroscopy; differential scanning calorimetry; enthalpy; enzyme activity; enzyme inhibition; fatty acids; hydrogen bonding; hydrophobic bonding; micelles; temperature; titration
Abstract:
... In this work, the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions were studied by using differential scanning calorimetry (DSC), circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), and docking simulation. The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critica ...
DOI:
10.1021/acs.jafc.7b04469

18. Cloning and characterization of two novel β-glucosidase genes encoding isoenzymes of the cellobiase complex from Cellulomonas biazotea

Author:
Anthony K.N. Chan; Alan K.L. Ng; Kate K.Y. Ng; W.K.R. Wong
Source:
Gene 2018 v.642 pp. 367-375
ISSN:
0378-1119
Subject:
Cellulomonas biazotea; Escherichia coli; amino acids; beta-glucosidase; biofuels; carbon; cellobiose; cellulose; cellulosic wastes; clones; energy; genes; hosts; hydrolysis; isozymes; plasmids; proteins; signal peptide; substrate specificity
Abstract:
... Enzymatic degradation of cellulosic waste to generate renewable biofuels has offered an attractive solution to the energy problem. Synergistic hydrolysis of cellulose residues requires the participation of three different types of cellulases – endoglucanases, exoglucanases, and β-glucosidases (Bgl). Our group has been interested in using Bgl of Cellulomonas biazotea in studies designed to investig ...
DOI:
10.1016/j.gene.2017.11.043

19. Complete genome sequencing of Arachidicoccus ginsenosidimutans sp. nov., and its application for production of minor ginsenosides by finding a novel ginsenoside-transforming β-glucosidase

Author:
Muhammad Zubair Siddiqi; Siddiqi Muhammad Shafi; Wan-Taek Im
Source:
RSC advances 2017 v.7 no.74 pp. 46745-46759
ISSN:
2046-2069
Subject:
Chitinophagaceae; Escherichia coli; Internet; amino acids; beta-glucosidase; chemotaxonomy; chromosomes; clones; genes; ginsenosides; new species; phenotype; phylogeny; sequence analysis
Abstract:
... A novel bacterial strain (BS20ᵀ), which has ginsenoside-transforming ability, was whole genome sequenced for the identification of a target gene. After complete genome sequencing, phylogenetic, phenotypic and chemotaxonomic analyses, the strain BS20ᵀ (Arachidicoccus ginsenosidimutans sp. nov.) was placed within the genus Arachidicoccus of family Chitinophagaceae. The complete genome of strain BS20 ...
DOI:
10.1039/c7ra02612a

20. Molecular Cloning and Characterization of a Putative β-glucosidase from the Formosan Subterranean Termite (Isoptera: Rhinotermitidae)

Author:
Xiaolin Liu; Wenjing Wu; Dandan Zhang; Zhiqiang Li
Source:
Journal of entomological science 2017 v.52 no.2 pp. 177-192
ISSN:
0749-8004
Subject:
Coptotermes formosanus; amino acids; beta-glucosidase; cellulose; complementary DNA; digestion; digestive enzymes; gene expression regulation; genes; hydrolysis; messenger RNA; models; molecular cloning; molecular weight; open reading frames; phylogeny; quantitative polymerase chain reaction; signal peptide; temperature
Abstract:
... Beta-glucosidases play an important role in the hydrolysis of cellulose and are among the cellulases in termites responsible for cellulose degradation and regulation of gene expression. A cDNA encoding putative β-glucosidase (CfBGIa) from the Formosan subterranean termite, Coptotermes formosanus Shiraki, was successfully cloned. The full-length of the cDNA of CfBGIa was 2,195 base pairs (bp) with ...
DOI:
10.18474/0749-8004-52.2.177

21. Multiple mutations in the aglycone binding pocket to convert the substrate specificity of dalcochinase to linamarase

Author:
Nusra Tongtubtim; Pornpanna Thenchartanan; Khakhanang Ratananikom; Khuanjarat Choengpanya; Jisnuson Svasti; Prachumporn T. Kongsaeree
Source:
Biochemical and biophysical research communications 2018 v.504 no.4 pp. 647-653
ISSN:
0006-291X
Subject:
1-propanol; Dalbergia cochinchinensis; Manihot esculenta; alkyl glucosides; amino acids; beta-glucosidase; carbon; energy; glucose; hydrolysis; kinetics; moieties; mutants; mutation; quantitative analysis; sequence analysis; substrate specificity
Abstract:
... Dalcochinase from Dalbergia cochinchinensis Pierre and linamarase from Manihot esculenta Crantz are β-glucosidases which share 47% sequence identity, but show distinct substrate specificities in hydrolysis and transglucosylation. Previously, three amino acid residues of dalcochinase, namely I185, N189 and V255, were identified as being important for determining substrate specificity. In this study ...
DOI:
10.1016/j.bbrc.2018.08.202

22. Purification and characterization of a novel GH1 beta-glucosidase from Jeotgalibacillus malaysiensis

Author:
Kok Jun Liew; Lily Lim; Hui Ying Woo; Kok-Gan Chan; Mohd Shahir Shamsir; Kian Mau Goh
Source:
International journal of biological macromolecules 2018 v.115 pp. 1094-1102
ISSN:
0141-8130
Subject:
Anoxybacillus; Elaeis guineensis; Escherichia coli; amino acids; beta-glucosidase; biofuels; calcium chloride; carboxymethylcellulose; cellobiose; fruits; genes; glycosides; molecular weight; pH; recombinant proteins; sequence homology; wastes; xylan
Abstract:
... Beta-glucosidase (BGL) is an important industrial enzyme for food, waste and biofuel processing. Jeotgalibacillus is an understudied halophilic genus, and no beta-glucosidase from this genus has been reported. A novel beta-glucosidase gene (1344 bp) from J. malaysiensis DSM 28777ᵀ was cloned and expressed in E. coli. The recombinant protein, referred to as BglD5, consists of a total 447 amino acid ...
DOI:
10.1016/j.ijbiomac.2018.04.156

23. Specific amino acids responsible for the cold adaptedness of Micrococcus antarcticus β-glucosidase BglU

Author:
Li-Li Miao; Hong-Xia Fan; Jie Qu; Ying Liu; Zhi-Pei Liu
Source:
Applied microbiology and biotechnology 2017 v.101 no.5 pp. 2033-2041
ISSN:
0175-7598
Subject:
Micrococcus antarcticus; amino acids; bacteria; beta-glucosidase; cold; crystal structure; engineering; food production; heat stability; industry; mutagenesis; mutants; sequence alignment; sewage treatment; surface area; temperature
Abstract:
... Psychrophilic enzymes display efficient activity at moderate or low temperatures (4–25 °C) and are therefore of great interest in biotechnological industries. We previously examined the crystal structure of BglU, a psychrophilic β-glucosidase from the bacterium Micrococcus antarcticus, at 2.2 Å resolution. In structural comparison and sequence alignment with mesophilic (BglB) and thermophilic (Gly ...
DOI:
10.1007/s00253-016-7990-x

24. Time-resolved comparative metabolomes for Koji fermentation with brown-, white-, and giant embryo-rice

Author:
Da Eun Lee; Sunmin Lee; Digar Singh; Eun Seok Jang; Hye Won Shin; Byoung Seok Moon; Choong Hwan Lee
Source:
Food chemistry 2017 v.231 pp. 258-266
ISSN:
0308-8146
Subject:
amino acids; antioxidant activity; beta-glucosidase; brown rice; fatty acids; fermentation; flavonoids; koji; metabolites; metabolome; metabolomics; phenolic acids; sugars; vitamins
Abstract:
... A time-resolved non-targeted metabolomic study towards evaluating the effects of three rice substrate types viz., white rice (WR), giant embryo rice (GER), and brown rice (BR), on Koji fermentation was performed. Notwithstanding the relatively higher proportions of metabolites in unfermented BR (BR 0>WR 0, GER 0), the relative levels of metabolic repertoire in BR Koji were marginally enhanced duri ...
DOI:
10.1016/j.foodchem.2017.03.119

25. Untargeted metabolite profiling for koji-fermentative bioprocess unravels the effects of varying substrate types and microbial inocula

Author:
Han Sol Seo; Sunmin Lee; Digar Singh; Hye Won Shin; Sun A Cho; Choong Hwan Lee
Source:
Food chemistry 2018 v.266 pp. 161-169
ISSN:
0308-8146
Subject:
Aspergillus oryzae; Bacillus amyloliquefaciens; alpha-amylase; amino acids; beta-glucosidase; bioprocessing; data collection; enzyme activity; fatty acids; fermented foods; flavones; ingredients; inoculum; koji; mass spectrometry; metabolomics; multivariate analysis; nucleosides; phenolic acids; rice; secondary metabolites; soybeans; sugar alcohols; vitamins; wheat
Abstract:
... Untargeted metabolomics unraveled the effects of varying substrates (soybean, wheat, and rice) and inocula (Aspergillus oryzae and Bacillus amyloliquefaciens) on metabolite compositions of koji, a starter ingredient in various Asian fermented foods. Multivariate analyses of the hyphenated mass spectrometry datasets for different koji extracts highlighted 61 significantly discriminant primary metab ...
DOI:
10.1016/j.foodchem.2018.05.048

26. Characterization of a new multifunctional beta-glucosidase from Musca domestica

Author:
Shu Zhang; Jian Huang; Rong Hu; Guo Guo; Xiaoli Shang; Jianwei Wu
Source:
Biotechnology letters 2017 v.39 no.8 pp. 1219-1227
ISSN:
0141-5492
Subject:
Komagataella pastoris; complementary DNA; glucose; ionization; cellobiose; rapid amplification of cDNA ends; cellulose; industrial applications; amino acids; genes; endo-1,4-beta-glucanase; glycosides; Musca domestica; beta-glucosidase; desorption
Abstract:
... OBJECTIVE: To engineer Pichia pastoris for heterologous production of cellulase from Musca domestica and explore its potential for industrial applications. RESULTS: A new beta-glucosidase gene (bg), encoding 562 amino acids, was cloned from M. domestica by using rapid amplification of cDNA ends. The gene bg was linked to pPICZαA and expressed in P. pastoris with a yield of 500 mg l⁻¹. The enzyme h ...
DOI:
10.1007/s10529-017-2351-0

27. Characterization of molecular signature of the roots of Paeonia lactiflora during growth

Author:
Pei LIU; Yuan XU; Hui YAN; Jing CHEN; Er-Xin SHANG; Da-Wei QIAN; Shu JIANG; Jin-Ao DUAN
Source:
Chinese journal of natural medicines 2017 v.15 no.10 pp. 785-793
ISSN:
1875-5364
Subject:
Oriental traditional medicine; Paeonia lactiflora; amino acids; beta-glucosidase; drugs; enzyme activity; esterases; gallic acid; glycosides; harvest date; health foods; high performance liquid chromatography; metabolites; monoterpenoids; nucleosides; phenols; polysaccharides; roots; tandem mass spectrometry; Asia
Abstract:
... The roots of Paeoniae lactiflora Pall. are widely consumed as crude drugs in Asian countries due to their remarkable beneficial health effects. The present research was undertaken to illuminate the dynamic changes in metabolites and enzymes and facilitate selection of the harvesting time when the herb can provide optimum health benefits. P. lactiflora roots were analyzed at 12 stages of growth for ...
DOI:
10.1016/S1875-5364(17)30110-3

28. Crystal structure and identification of a key amino acid for glucose tolerance, substrate specificity, and transglycosylation activity of metagenomic β‐glucosidase Td2F2

Author:
Tomohiko Matsuzawa; Toshinori Jo; Taku Uchiyama; Jenny A. Manninen; Takatoshi Arakawa; Kentaro Miyazaki; Shinya Fushinobu; Katsuro Yaoi
Source:
FEBS journal 2016 v.283 no.12 pp. 2340-2353
ISSN:
1742-464X
Subject:
amino acids; beta-glucosidase; composts; crystal structure; databases; glucose tolerance; glycosides; metagenomics; mutagenesis; mutants; substrate specificity
Abstract:
... β‐Glucosidase Td2F2 isolated from a compost metagenome has high glucose tolerance and transglycosylation activity. In this study, we determined the high‐resolution crystal structure of Td2F2. It has a unique structure at the −1 subsite that is important for substrate specificity but not for glucose tolerance. To elucidate the mechanism(s) of glucose tolerance, we isolated a glucose‐sensitive Td2F2 ...
DOI:
10.1111/febs.13743

29. Molecular cloning, characterization and in silico analysis of a thermostable β-glucosidase enzyme from Putranjiva roxburghii with a significant activity for cellobiose

Author:
Bibekananda Kar; Preeti Verma; Girijesh Kumar Patel; Ashwani Kumar Sharma
Source:
Phytochemistry 2017 v.140 pp. 151-165
ISSN:
0031-9422
Subject:
Escherichia coli; Lewis bases; Putranjiva roxburghii; affinity chromatography; amino acids; beta-galactosidase; beta-glucosidase; bioinformatics; catalytic activity; cellobiose; enzyme activity; gene overexpression; genes; glucose; molecular cloning; open reading frames; polypeptides; recombinant proteins; sequence homology; substrate specificity; thermal stability
Abstract:
... The native Putranjiva roxburghii family 1 glycoside hydrolase enzyme showed β-D-fucosidase activity in addition to β-D-glucosidase and β-D-galactosidase activities reported in our previous study. A single step concanvalin A affinity chromatography for native PRGH1 improved the yield and reduced the purification time. The PRGH1 gene was cloned and overexpressed in E. coli. The full length gene cont ...
DOI:
10.1016/j.phytochem.2017.04.021

30. Response of hydrolytic enzyme activities and nitrogen mineralization to fertilizer and organic matter application in subtropical paddy soils

Author:
M.A. Kader; S. Yeasmin; Z.M. Solaiman; S. De Neve; S. Sleutel
Source:
European journal of soil biology 2017 v.80 pp. 27-34
ISSN:
1164-5563
Subject:
agricultural colleges; alluvial soils; amino acids; animal manures; beta-glucosidase; correlation; crop rotation; enzyme activity; farms; fertilizer application; field experimentation; hydrolysis; microbial activity; mineralization; nitrogen; nitrogen fertilizers; organic matter; paddy soils; rice; terrace soils; terraces; urease; wheat; Bangladesh
Abstract:
... Drivers of nitrogen (N) mineralization in paddy soils, especially under anaerobic soil conditions, are elusive. The influences of exogenous organic matter (OM) and fertilizer application on the activities of five relevant enzymes (β-glucosaminidase, β-glucosidase, l-glutaminase, urease and arylamidase) were measured in two long-term field experiments. Of the two field experiments, the 18-year fiel ...
DOI:
10.1016/j.ejsobi.2017.03.004

31. A Novel Soybean (Glycine max) Gene Encoding a Family 3 β-Glucosidase Has High Isoflavone 7-O-Glucoside-Hydrolyzing Activity in Transgenic Rice

Author:
Chia-Chen Hsu; Tsung-Meng Wu; Yi-Ting Hsu; Chih-Wen Wu; Chwan-Yang Hong; Nan-Wei Su
Source:
Journal of agricultural and food chemistry 2015 v.63 no.3 pp. 921-928
ISSN:
0021-8561
Subject:
Glycine max; Oryza sativa; amino acid sequences; amino acids; beta-glucosidase; complementary DNA; conserved sequences; daidzein; daidzin; enzyme activity; genes; genetic engineering; genistein; genistin; hydrolysis; leaves; molecular weight; open reading frames; phylogeny; polypeptides; rice; roots; seeds; sequence analysis; soybeans; substrate specificity; transgenic plants
Abstract:
... A previous study demonstrated that purified Glycine max β-glucosidase (GmBGL) could hydrolyze glucosyl isoflavone to the aglyconic form. This study reports the cloning and functional characterization of a soybean cDNA encoding the β-glucosidase. GmBGL was isolated by use of a purified soybean N-terminal amino acid sequence and conserved sequences of β-glucosidase genes from other plants. Sequen ...
DOI:
10.1021/jf504778x

32. A Solanum torvum GH3 β-glucosidase expressed in Pichia pastoris catalyzes the hydrolysis of furostanol glycoside

Author:
Rungarun Suthangkornkul; Pornpisut Sriworanun; Hiroyuki Nakai; Masayuki Okuyama; Jisnuson Svasti; Atsuo Kimura; Saengchan Senapin; Dumrongkiet Arthan
Source:
Phytochemistry 2016 v.127 pp. 4-11
ISSN:
0031-9422
Subject:
quantitative polymerase chain reaction; Solanum torvum; seeds; Komagataella pastoris; complementary DNA; glycosylation; open reading frames; seedlings; substrate specificity; reverse transcriptase polymerase chain reaction; rapid amplification of cDNA ends; amino acid sequences; amino acids; hydrolysis; catalytic activity; glycosides; beta-glucosidase; leaves
Abstract:
... Plant β-glucosidases are usually members of the glucosyl hydrolase 1 (GH1) or 3 (GH3) families. Previously, a β-glucosidase (torvosidase) was purified from Solanum torvum leaves that specifically catalyzed hydrolysis of two furostanol 26-O-β-glucosides, torvosides A and H. Furostanol glycoside 26-O-β-glucosides have been reported as natural substrates of some plant GH1 enzymes. However, torvosidas ...
DOI:
10.1016/j.phytochem.2016.03.015

33. Alkali-tolerant β-glucosidase produced by newly isolated Aspergillus fumigatus WL002 from rotten wood

Author:
Pinghua Cao; Lei Wang; Yaoyue Wang; Ning Zhou; Yulin Chen
Source:
International Biodeterioration & Biodegradation 2015 v.105 pp. 276-282
ISSN:
0964-8305
Subject:
lignocellulose; polyacrylamide gel electrophoresis; nucleotides; Aspergillus fumigatus; genes; polypeptides; open reading frames; internal transcribed spacers; amino acids; active sites; molecular weight; metal ions; beta-glucosidase; carboxymethylcellulose; Escherichia coli; polymerase chain reaction; biotransformation; temperature; wood; pH; biodegradation; fungi
Abstract:
... A lignocellulosic decomposing fungus WL002 with a high β-glucosidase activity was isolated from rotten wood. The fungus was classified and designated as Aspergillus fumigatus WL002 through morphological and internal transcribed spacer sequence (ITS) analysis. A putative β-glucosidase gene, bglW, which was cloned from the isolated strain by PCR amplification, consists of an open reading frame of 25 ...
DOI:
10.1016/j.ibiod.2015.09.010

34. Biotransformation of gypenoside XVII to compound K by a recombinant β-glucosidase

Author:
Fei-Liang Zhong; Wei-Wei Dong; Songquan Wu; Jun Jiang; Deok-Chun Yang; Donghao Li; Lin-Hu Quan
Source:
Biotechnology letters 2016 v.38 no.7 pp. 1187-1193
ISSN:
0141-5492
Subject:
Escherichia coli; Lactobacillus brevis; amino acids; beta-glucosidase; biotransformation; genes; glucose; pH; sodium phosphate
Abstract:
... OBJECTIVE: To study the β-glucosidase gene (bgy1) from Lactobacillus brevis that was cloned and expressed in Escherichia coli BL21 (DE3) and then using it for the biotransformation of gypenoside XVII. RESULTS: The bgy1 gene consists of 2283 bp encoding 761 amino acids, with homology to the glycosyl hydrolase family-3 protein domain. The enzyme (Bgy1) hydrolyzed the glucose moieties at the C-3 posi ...
DOI:
10.1007/s10529-016-2094-3

35. Characterization of a novel manganese dependent endoglucanase belongs in GH family 5 from Phanerochaete chrysosporium

Author:
Nguyen Duc Huy; Cu Le Nguyen; Han-Sung Park; Nguyen Hoang Loc; Myoung-Suk Choi; Dae-Hyuk Kim; Jeong-Woo Seo; Seung-Moon Park
Source:
Journal of bioscience and bioengineering 2016 v.121 no.2 pp. 154-159
ISSN:
1389-1723
Subject:
manganese; thermal stability; Komagataella pastoris; polyacrylamide gel electrophoresis; cellulose 1,4-beta-cellobiosidase; glucose; amino acids; active sites; carbohydrate binding; molecular weight; beta-glucosidase; saccharification; complementary DNA; temperature; enzyme activity; biomass; Phanerochaete chrysosporium; glycosides; barley straw; pH; endo-1,4-beta-glucanase
Abstract:
... The cDNA encoding a putative glycoside hydrolase family 5, which has been predicted to be an endoglucanase (PcEg5A), was cloned from Phanerochaete chrysosporium and expressed in Pichia pastoris. PcEg5A contains a carbohydrate-binding domain and two important amino acids, E209 and E319, playing as proton donor and nucleophile in substrate catalytic domain. SDS-PAGE analysis indicated that the recom ...
DOI:
10.1016/j.jbiosc.2015.06.009

36. Cloning with kinetic and thermodynamic insight of a novel hyperthermostable β-glucosidase from Thermotoga naphthophila RKU-10T with excellent glucose tolerance

Author:
Fatima Akram; Ikram ul Haq; Mahmood Ali Khan; Zahid Hussain; Hamid Mukhtar; Kaleem Iqbal
Source:
Journal of molecular catalysis 2016 v.124 pp. 92-104
ISSN:
1381-1177
Subject:
Escherichia coli; Thermotoga; amino acids; bacteria; beta-glucosidase; biomass; catalytic activity; cations; cellobiose; denaturation; detergents; enzyme activity; gene overexpression; genes; glucose; glucose tolerance; glycosides; half life; heat tolerance; heat treatment; hydrolysis; hydrophobic interaction chromatography; industrial applications; ion exchange; molecular weight; pH; renewable resources; temperature; thermal stability
Abstract:
... With a paradigm shift in industry, moving from natural fuels to alternative renewable resource utilization, the need of efficient thermostable cellulases are expected to increase in future. β-glucosidase, an essential member of cellulases that plays a critical role in cellulosic biomass degradation and in many biological processes. Therefore, a novel β-glucosidase gene encodes a protein (BglA) of ...
DOI:
10.1016/j.molcatb.2015.12.005

37. Discovery of a Bacterial Glycoside Hydrolase Family 3 (GH3) β-Glucosidase with Myrosinase Activity from a Citrobacter Strain Isolated from Soil

Author:
Abdulhadi Albaser; Eleanna Kazana; Mark H. Bennett; Fatma Cebeci; Vijitra Luang-In; Pietro D. Spanu; John T. Rossiter
Source:
Journal of agricultural and food chemistry 2016 v.64 no.7 pp. 1520-1527
ISSN:
0021-8561
Subject:
Citrobacter; amino acids; beta-glucosidase; carbon; enzyme activity; gel chromatography; genes; ion exchange; molecular weight; pH; sequence analysis; signal peptide; sinigrin; soil; temperature; thioglucosidase; United Kingdom
Abstract:
... A Citrobacter strain (WYE1) was isolated from a UK soil by enrichment using the glucosinolate sinigrin as sole carbon source. The enzyme myrosinase was purified using a combination of ion exchange and gel filtration to give a pure protein of approximately 66 kDa. The N-terminal amino acid and internal peptide sequence of the purified protein were determined and used to identify the gene, which, ba ...
DOI:
10.1021/acs.jafc.5b05381

38. Elevated atmospheric CO2 affected photosynthetic products in wheat seedlings and biological activity in rhizosphere soil under cadmium stress

Author:
Xia Jia; Tuo Liu; Yonghua Zhao; Yunhua He; Mingyan Yang
Source:
Environmental science and pollution research international 2016 v.23 no.1 pp. 514-526
ISSN:
0944-1344
Subject:
Actinomycetales; amino acids; asparaginase; beta-glucosidase; bioactive properties; cadmium; carbon dioxide; carbon dioxide enrichment; endo-1,4-beta-glucanase; enzyme activity; fluorescein; free amino acids; fungi; heavy metals; monophenol monooxygenase; phenolic acids; pollution; reducing sugars; rhizosphere; seedlings; soil; soil fertility; starch; urease; wheat; wheat protein
Abstract:
... The objective of this study was to investigate the effects of elevated CO₂ (700 ± 23 μmol mol⁻¹) on photosynthetic products in wheat seedlings and on organic compounds and biological activity in rhizosphere soil under cadmium (Cd) stress. Elevated CO₂ was associated with decreased quantities of reducing sugars, starch, and soluble amino acids, and with increased quantities of soluble sugars, total ...
DOI:
10.1007/s11356-015-5288-7

39. Endogenous cellulase production in the leaf litter foraging mangrove crab Parasesarma erythodactyla

Author:
T.H. Hanh Bui; Shing Yip Lee
Source:
Comparative Biochemistry and Physiology, Part B 2015 v.179 pp. 27-36
ISSN:
1096-4959
Subject:
DNA fragmentation; Uca; Varunidae; active sites; amino acids; arthropods; beta-glucosidase; cellulose; complementary DNA; crabs; detritivores; digestion; digestive juices; endo-1,4-beta-glucanase; exons; foraging; hepatopancreas; introns; molecular cloning; muscle tissues; open reading frames; pH; plant litter; polymerase chain reaction; sequence analysis; temperature
Abstract:
... The sesarmid crab Parasesarma erythodactyla consumes large amounts of mangrove leaf litter but its biochemical capacity for cellulose digestion is poorly known. We demonstrate the presence of endo-β-1,4-glucanase, β-glucosidase and total cellulase activities in the digestive juice of this crab. The highest total cellulase activity was observed at mildly acidic pH (5 to 6) and temperature between 3 ...
DOI:
10.1016/j.cbpb.2014.09.004

40. Enzyme-assisted subcritical water extraction and characterization of soy protein from heat-denatured meal

Author:
Wei Lu; Xiao-Wei Chen; Jin-Mei Wang; Xiao-Quan Yang; Jun-Ru Qi
Source:
Journal of food engineering 2016 v.169 pp. 250-258
ISSN:
0260-8774
Subject:
Aspergillus oryzae; Maillard reaction; adsorption; amino acids; beta-glucosidase; emulsifying; emulsifying properties; emulsions; food industry; functional foods; glycosides; hydrolysis; hydrophobicity; isoflavones; protein unfolding; proteinases; soy protein isolate; soybean meal; surface proteins
Abstract:
... Protease prehydrolysis followed by subcritical water (SW) treatment was carried out to extract protein from heat-denatured soy meal. The composition, physicochemical, interfacial and emulsifying properties of soy protein isolate (SPI) were evaluated. A significant increase in extraction yield was observed although protein purity was decreased with prolonged hydrolysis. Due to contaminated β-glucos ...
DOI:
10.1016/j.jfoodeng.2015.09.006

41. Heterologous Expression and Characterization of a GH3 β-Glucosidase from Thermophilic Fungi Myceliophthora thermophila in Pichia pastoris

Author:
Junqi Zhao; Chao Guo; Chaoguang Tian; Yanhe Ma
Source:
Applied biochemistry and biotechnology 2015 v.177 no.2 pp. 511-527
ISSN:
0273-2289
Subject:
thermal stability; molecular models; Komagataella pastoris; Myceliophthora thermophila; cellobiose; nucleotides; amino acids; thermophilic fungi; genes; pH; enzyme substrates; heterologous gene expression; glycosides; beta-glucosidase; gentiobiose
Abstract:
... A novel β-glucosidase of glycoside hydrolase (GH) family 3 from Myceliophthora thermophila (mtbgl3b) was successfully expressed in Pichia pastoris. The full-length gene consists of 2613 bp nucleotides encoding a protein of 870 amino acids. MtBgl3b showed maximum activity at pH 5.0 and remained more than 70 % relative activity at 3.5–6.0. The enzyme displayed the highest activity at 60 °C and kept ...
DOI:
10.1007/s12010-015-1759-z

42. Lignocellulose degrading extremozymes produced by Pichia pastoris: current status and future prospects

Author:
Burcu Gündüz Ergün; Pınar Çalık
Source:
Bioprocess and biosystems engineering 2016 v.39 no.1 pp. 1-36
ISSN:
1615-7591
Subject:
engineering; thermal stability; protein folding; lignocellulose; Komagataella pastoris; xylanases; glycosylation; beta-mannosidase; models; metagenomics; amino acids; molecular dynamics; site-directed mutagenesis; catalytic activity; beta-glucosidase; prediction
Abstract:
... In this review article, extremophilic lignocellulosic enzymes with special interest on xylanases, β-mannanases, laccases and finally cellulases, namely, endoglucanases, exoglucanases and β-glucosidases produced by Pichia pastoris are reviewed for the first time. Recombinant lignocellulosic extremozymes are discussed from the perspectives of their potential application areas; characteristics of rec ...
DOI:
10.1007/s00449-015-1476-6

43. Rice bran protein hydrolysates exhibit strong in vitro α‐amylase, β‐glucosidase and ACE‐inhibition activities

Author:
Chatchaporn Uraipong; Jian Zhao
Source:
Journal of the science of food and agriculture 2016 v.96 no.4 pp. 1101-1110
ISSN:
0022-5142
Subject:
acarbose; albumins; alpha-amylase; alpha-glucosidase; amino acids; beta-glucosidase; bioactive properties; diabetes; functional foods; glutelins; health promotion; hydrolysates; hydrolysis; hypoglycemic agents; ion exchange chromatography; peptides; peptidyl-dipeptidase A; prolamins; protein hydrolysates; rice bran; subtilisin
Abstract:
... BACKGROUND: The objective of this study was to systematically examine the in vitro health‐promotion activities of rice bran protein hydrolysates. Rice bran proteins were fractioned into albumin, globulin, prolamin and glutelin, which were subjected to hydrolysis by four protease preparations, namely Alcalase, Neutrase, Flavourzyme and Protamax, and the inhibitory activities of the hydrolysates aga ...
DOI:
10.1002/jsfa.7182

44. Secretory expression, characterization and docking study of glucose-tolerant β-glucosidase from B. subtilis

Author:
Shivangi Chamoli; Piyush Kumar; Naveen Kumar Navani; Ashok Kumar Verma
Source:
International journal of biological macromolecules 2016 v.85 pp. 425-433
ISSN:
0141-8130
Subject:
Bacillus subtilis; Escherichia coli; active sites; amino acids; beta-glucosidase; catalytic activity; detergents; gene overexpression; genes; gluconolactone; glucose; glycosides; hydrogen bonding; hydrophobic bonding; industrial applications; kinetics; models; molecular weight; open reading frames; pH; recombinant proteins; secretion; solvents; temperature; thermal stability
Abstract:
... The thermostable, glucose tolerant β-glucosidase gene (bgl) of Glycoside hydrolase family 1, isolated from Bacillus subtilis, was cloned and overexpressed in Escherichia coli. The bgl has open reading frame of 1407bp, encoding 469 amino acids with predicted molecular weight of 53kDa. The recombinant protein (BGL) was purified 10.76 fold to homogeneity with specific activity of 54.04U/mg and recove ...
DOI:
10.1016/j.ijbiomac.2016.01.001

45. Site-saturation mutagenesis for β-glucosidase 1 from Aspergillus aculeatus to accelerate the saccharification of alkaline-pretreated bagasse

Author:
Yutaro Baba; Jun-ichi Sumitani; Kiyotaka Tanaka; Shuji Tani; Takashi Kawaguchi
Source:
Applied microbiology and biotechnology 2016 v.100 no.24 pp. 10495-10507
ISSN:
0175-7598
Subject:
Aspergillus aculeatus; Lewis bases; Trichoderma reesei; amino acids; bagasse; beta-glucosidase; biomass; catalytic activity; cellobiose; gentiobiose; kinetics; mutagenesis; mutants; pH; saccharification; xylanases
Abstract:
... Aspergillus aculeatus β-glucosidase 1 (AaBGL1) is one of the best cellobiose hydrolytic enzymes without transglycosylation products, among β-glucosidase from various origins, for use in cellulosic biomass conversion with Trichoderma cellulases. However, in our previous report, it was demonstrated that AaBGL1 has lower catalytic efficiency toward cellobiose, which is a major end product from cellul ...
DOI:
10.1007/s00253-016-7726-y

46. The effects of sediment depth and oxygen concentration on the use of organic matter: An experimental study using an infiltration sediment tank

Author:
A. Freixa; S. Rubol; A. Carles-Brangarí; D. Fernàndez-Garcia; A. Butturini; X. Sanchez-Vila; A.M. Romaní
Source:
Science of the total environment 2016 v.540 pp. 20-31
ISSN:
0048-9697
Subject:
amino acids; anaerobic conditions; beta-glucosidase; carbon; carboxylic acids; enzyme activity; enzyme inhibition; extracellular enzymes; groundwater recharge; hyporheic zone; metabolism; microbial communities; microorganisms; nitrogen compounds; organic matter; oxygen; rivers; sediments
Abstract:
... Water flowing through hyporheic river sediments or artificial recharge facilities promotes the development of microbial communities with sediment depth. We performed an 83-day mesocosm infiltration experiment, to study how microbial functions (e.g., extracellular enzyme activities and carbon substrate utilization) are affected by sediment depth (up to 50cm) and different oxygen concentrations. Res ...
DOI:
10.1016/j.scitotenv.2015.04.007

47. Transglycosylating glycoside hydrolase family 1 β-glucosidase from Penicillium funiculosum NCL1: Heterologous expression in Escherichia coli and characterization

Author:
Gurusamy Ramani; Balasubramanian Meera; Jeyaprakash Rajendhran; Paramasamy Gunasekaran
Source:
Biochemical engineering journal 2015 v.102 pp. 6-13
ISSN:
1369-703X
Subject:
EDTA (chelating agent); Escherichia coli; RNA; Talaromyces funiculosus; amino acids; beta-glucosidase; calcium; cations; cellobiose; complementary DNA; fungi; gene overexpression; genes; gentiobiose; glucose; glucose tolerance; glycosides; heterologous gene expression; lactose; magnesium; manganese; molecular weight; open reading frames; pH; prebiotics; reverse transcriptase polymerase chain reaction; temperature; zinc
Abstract:
... A β-glucosidase cDNA belonging to glycoside hydrolase family 1 was cloned from RNA isolated from lactose grown cellulolytic fungus, Penicillium funiculosum NCL1 by reverse-transcriptase polymerase chain reaction. The gene (bgl6) contained an open reading frame of 1470bp that encoded 490 amino acids. bgl6 gene was overexpressed in Escherichia coli BL21-CodonPlus and the recombinant enzyme (rBgl6) w ...
DOI:
10.1016/j.bej.2015.03.018

48. A Novel Three Domains Glycoside Hydrolase Family 3 from Sclerotinia sclerotiorum Exhibits β-Glucosidase and Exoglucanase Activities: Molecular, Biochemical, and Transglycosylation Potential Analysis

Author:
Haifa Chahed; Aymen Ezzine; Mohamed Amine Ben Mlouka; Christophe Rihouey; Julie Hardouin; Thierry Jouenne; M. Nejib Marzouki
Source:
Molecular biotechnology 2015 v.57 no.11-12 pp. 993-1002
ISSN:
1073-6085
Subject:
Sclerotinia sclerotiorum; active sites; amino acids; beta-glucosidase; crystal structure; fibronectins; fungi; gel chromatography; glucooligosaccharides; mass spectrometry; molecular models; molecular weight; pH; polyacrylamide gel electrophoresis; polypeptides
Abstract:
... The filamentous fungus Sclerotinia sclerotiorum produces a complete set of cellulolytic enzymes. We report here the purification and the biochemical characterization of a new β-glucosidase from S. sclerotiorum which belongs to the family 3 of glycoside hydrolases and that was named as SsBgl3. After two size-exclusion chromatography steps, purified protein bands of 80 and 90 kDa from SDS-PAGE were ...
DOI:
10.1007/s12033-015-9892-z

49. Characterization of recombinant β-glucosidase from Arthrobacter chlorophenolicus and biotransformation of ginsenosides Rb₁, Rb ₂, Rc, and Rd

Author:
Myung Keun Park; Chang-Hao Cui; Sung Chul Park; Seul-Ki Park; Jin-Kwang Kim; Mi-Sun Jung; Suk-Chae Jung; Sun-Chang Kim; Wan-Taek Im
Source:
journal of microbiology 2014 v.52 no.5 pp. 399-406
ISSN:
1225-8873
Subject:
Arthrobacter chlorophenolicus; Escherichia coli; agarose; amino acids; beta-glucosidase; biotransformation; gene overexpression; genes; ginsenosides; glucose; hydrolysis; industry; microbiology; pH
Abstract:
... The focus of this study was the cloning, expression, and characterization of recombinant ginsenoside hydrolyzing β-glucosidase from Arthrobacter chlorophenolicus with an ultimate objective to more efficiently bio-transform ginsenosides. The gene bglAch, consisting of 1,260 bp (419 amino acid residues) was cloned and the recombinant enzyme, overexpressed in Escherichia coli BL21 (DE3), was characte ...
DOI:
10.1007/s12275-014-3601-7

50. Effects of the glyphosate-resistance gene and of herbicides applied to the soybean crop on soil microbial biomass and enzymes

Author:
André Shigueyoshi Nakatani; Marcelo Ferreira Fernandes; Rosinei Aparecida de Souza; Adriana Pereira da Silva; Fábio Bueno dos Reis-Junior; Iêda Carvalho Mendes; Mariangela Hungria
Source:
Field crops research 2014 v.162 pp. 20-29
ISSN:
0378-4290
Subject:
3-phosphoshikimate 1-carboxyvinyltransferase; acid phosphatase; amino acids; bacteria; beta-glucosidase; cultivars; enzyme activity; field experimentation; fungi; genetic engineering; glyphosate; herbicide resistance; microbial biomass; multivariate analysis; pesticide application; proteins; shikimic acid; soil; soil microorganisms; soybeans; transgenes; transgenic plants; weed control; Brazil
Abstract:
... Glyphosate, a broad-spectrum herbicide used for the non-selective control of weeds, inhibits 5-enolpyruvylshikimate-3-phosphate synthase, a key enzyme in the synthesis of aromatic amino acids in the shikimic acid pathway in plants, fungi and bacteria, thus impairing the synthesis of proteins required for various life processes. Soybean genetically engineered to be glyphosate resistant (GR or Round ...
DOI:
10.1016/j.fcr.2014.03.010

51. Enhanced soluble expression of a thermostble β-glucosidase from Thermotoga maritima in Escherichia coli and its applicaton in immobilization

Author:
Y. M. Xue; C. Y. Xu; J. J. Hou; X. Q. Li; Z. G. Cao
Source:
Applied biochemistry and microbiology 2015 v.51 no.3 pp. 306-315
ISSN:
0003-6838
Subject:
temperature; thermal stability; immobilized enzymes; dairy products; chitinase; amino acids; production costs; genes; Thermotoga maritima; Thermoanaerobacterium thermosaccharolyticum; culture media; beta-glucosidase; chitin; Escherichia coli
Abstract:
... The β-glucosidase Tm-bglA gene from hyperthermophile Thermotoga maritima was cloned into expression vectors pET-28a, producing two proteins of 55 kDa and 52 kDa in cell, which could be referred to Tm-BglA with and without 23 amino acids. The results showed that fusion of 23 amino acids to Tm-BglA improved its soluble expression and product tolerance, resulting over 60% yield of recombinant Tm-BglA ...
DOI:
10.1134/S0003683815030175

52. Enzymatic transformation of vina-ginsenoside R7 to rare notoginsenoside ST-4 using a new recombinant glycoside hydrolase from Herpetosiphon aurantiacus

Author:
Ru-Feng Wang; Ming-Min Zheng; Yue-De Cao; Hao Li; Chun-Xiu Li; Jian-He Xu; Zheng-Tao Wang
Source:
Applied microbiology and biotechnology 2015 v.99 no.8 pp. 3433-3442
ISSN:
0175-7598
Subject:
Herpetosiphon aurantiacus; amino acids; beta-glucosidase; biotechnology; ginsenosides; glucose; microbiology; thermal stability; xylose
Abstract:
... An eco-friendly and convenient preparation method for notoginsenoside ST-4 has been established by completely transforming vina-ginsenoside R₇using a recombinant glycosidase hydrolyzing enzyme (HaGH03) from Herpetosiphon aurantiacus. This enzyme specifically hydrolyzed the glucose at the C-20 position but not the external xylose or two inner glucoses at position C-3. Protein sequence BLAST reveale ...
DOI:
10.1007/s00253-015-6446-z

53. Expression and characterization of a novel β-glucosidase, with transglycosylation and exo-β-1,3-glucanase activities, from Rhizomucor miehei

Author:
Yu Guo; Qiaojuan Yan; Yu Yang; Shaoqing Yang; Yu Liu; Zhengqiang Jiang
Source:
Food chemistry 2015 v.175 pp. 431-438
ISSN:
0308-8146
Subject:
Escherichia coli; Rhizomucor miehei; amino acid sequences; amino acids; beta-glucosidase; gel chromatography; genes; gentiobiose; glucose; glycosides; industrial applications; molecular weight; open reading frames; pH; polyacrylamide gel electrophoresis; substrate specificity; temperature; thermophilic fungi
Abstract:
... A novel β-glucosidase gene, designated RmBglu3B, was cloned from the thermophilic fungus, Rhizomucor miehei CAU432. Its 2196-bp open reading frame encoded 731 amino acids. Its deduced amino-acid sequence showed highest identity (66%) with a glycoside hydrolase family 3 β-glucosidase from R. miehei NRRL5382. RmBglu3B was successfully expressed in Escherichia coli. The recombinant enzyme was purifie ...
DOI:
10.1016/j.foodchem.2014.12.004

54. Fluorescent probes for the detection of cyanide ions in aqueous medium: cellular uptake and assay for β-glucosidase and hydroxynitrile lyase

Author:
Hridesh Agarwalla; Monalisa Gangopadhyay; Dharmendar Kumar Sharma; Santanu Kumar Basu; Sameer Jadhav; Arindam Chowdhury; Amitava Das
Source:
Journal of materials chemistry B 2015 v.3 no.47 pp. 9148-9156
ISSN:
2050-7518
Subject:
World Health Organization; amino acids; anions; beta-glucosidase; cellular microenvironment; chemistry; detection limit; drinking water; energy; fluorescence; fluorescence microscopy; fluorescent dyes; glutathione; hydrogen cyanide; image analysis; membrane permeability; statistical analysis; toxicity
Abstract:
... A chemodosimteric reagent (1) for the efficient detection of cyanide species (CN⁻ and/or HCN) in aq. medium as well as under physiological conditions has been described. Selective reaction of the cyanide species with this reagent in the presence of all common interfering anions, amino acids and glutathione (GSH) led to the generation of the corresponding cyanohydrin derivative. The formation of th ...
DOI:
10.1039/c5tb01853f

55. Identification and characterization of prunasin hydrolases in sweet and bitter almonds and their expression in Nicotiana benthamiana plants

Author:
Cueto, J. del; Dicenta, F.; Jørgensen, K.; Møller, B.L.; Sánchez-Pérez, R.
Source:
Acta horticulturae 2014 no.1028 pp. 83-89
ISSN:
0567-7572
Subject:
seedlings; Nicotiana benthamiana; nucleotides; gels; glucosides; clones; amino acids; genotype; almonds; beta-glucosidase; nucellus; amino acid sequences; enzyme activity; cyanides; taste; bitterness; amygdalin; breeding; reverse transcriptase polymerase chain reaction; cotyledons; genetic variation; RNA; agroinfiltration
Abstract:
... Almond bitterness, caused by high amygdalin content, is the most important trait since bitter kernelled seedlings are usually discarded from breeding programs. Amygdalin and its precursor prunasin, both cyanogenic glucosides, are degraded by specific enzymes called β-glucosidases. In order to deep in the genetic control of almond bitterness some works showed differences in the localization of prun ...

56. Metabolism, excretion and avoidance of cyanogenic glucosides in insects with different feeding specialisations

Author:
Stefan Pentzold; Mika Zagrobelny; Nanna Bjarnholt; Juergen Kroymann; Heiko Vogel; Carl Erik Olsen; Birger Lindberg Møller; Søren Bak
Source:
Insect biochemistry and molecular biology 2015 v.66 pp. 119-128
ISSN:
0965-1748
Subject:
Lipaphis erysimi; Mamestra brassicae; Myzus persicae; Pieris brassicae; Pieris rapae; Plutella xylostella; Spodoptera exigua; Spodoptera littoralis; amino acids; beta-glucosidase; diet; excretion; glucosides; herbivores; hydrogen cyanide; insects; metabolism; plant tissues; rearing; toxicity
Abstract:
... Cyanogenic glucosides (CNglcs) are widespread plant defence compounds releasing toxic hydrogen cyanide when hydrolysed by specific β-glucosidases after plant tissue damage. In contrast to specialist herbivores that have mechanisms to avoid toxicity from CNglcs, it is generally assumed that non-adapted herbivores are negatively affected by CNglcs. Recent evidence, however, implies that the defence ...
DOI:
10.1016/j.ibmb.2015.10.004

57. Molecular Cloning of Five β-Glucosidases from Four Species of Higher Termites (Blattodea: Termitidae)

Author:
Nurmastini Sufina Bujang; Nigel A. Harrison; Nan-Yao Su
Source:
Annals of the Entomological Society of America 2014 v.107 no.1 pp. 251-256
ISSN:
0013-8746
Subject:
Macrotermes; biosphere; xylanases; glycosylation; amino acid sequences; cellulose; amino acids; digestive enzymes; molecular cloning; polymers; amylases; catalytic activity; beta-glucosidase; Blattodea
Abstract:
... Cellulose is the most abundant polymer in the biosphere, and termites are one of the most important metazoan cellulose processors. Termites are a rich source of digestive enzymes such as endo- β-1,4-glucanases, β-glucosidases, xylanases, amylases, pectinases, and laccases, regardless of whether they are termite derived or of microbiotic origin. Molecular identification of the termite's digestive e ...
DOI:
10.1603/AN13012

58. Molecular cloning and expression of thermostable glucose-tolerant β-glucosidase of Penicillium funiculosum NCL1 in Pichia pastoris and its characterization

Author:
Gurusamy Ramani; Balasubramanian Meera; Chinnathambi Vanitha; Jeyaprakash Rajendhran; Paramasamy Gunasekaran
Source:
Journal of industrial microbiology & biotechnology 2015 v.42 no.4 pp. 553-565
ISSN:
1367-5435
Subject:
matrix-assisted laser desorption-ionization mass spectrometry; thermal stability; RNA; Komagataella pastoris; profitability; genes; heat tolerance; glucose; recombinant proteins; Talaromyces funiculosus; cellulose; amino acids; active sites; molecular weight; glycoproteins; beta-glucosidase; complementary DNA; gene overexpression; industrial microbiology; cellobiose; ethanol production; hydrolysis; molecular cloning; Trichoderma reesei; glycosides; glucose tolerance; reverse transcriptase polymerase chain reaction; pH; endo-1,4-beta-glucanase; desorption
Abstract:
... A partial peptide sequence of β-glucosidase isoform (Bgl4) of Penicillium funiculosum NCL1 was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The cDNA (bgl4) encoding Bgl4 protein was cloned from P. funiculosum NCL1 RNA by consensus RT-PCR. The bgl4 gene encoded 857 amino acids that contained catalytic domains specific for glycoside hydrolase family 3. ...
DOI:
10.1007/s10295-014-1549-6

59. Overexpression and characterization of a glucose-tolerant β-glucosidase from T. aotearoense with high specific activity for cellobiose

Author:
Fang Yang; Xiaofeng Yang; Zhe Li; Chenyu Du; Jufang Wang; Shuang Li
Source:
Applied microbiology and biotechnology 2015 v.99 no.21 pp. 8903-8915
ISSN:
0175-7598
Subject:
Escherichia coli; Thermoanaerobacterium aotearoense; amino acids; beta-glucosidase; bioethanol; biomass; cellobiose; endo-1,4-beta-glucanase; enzyme activity; genes; glucose; glycosides; hydrolysis; lignocellulose; open reading frames; pH; potassium; reducing sugars; sodium; sugarcane bagasse; thermal stability
Abstract:
... Thermoanaerobacterium aotearoense P8G3#4 produced β-glucosidase (BGL) intracellularly when grown in liquid culture on cellobiose. The gene bgl, encoding β-glucosidase, was cloned and sequenced. Analysis revealed that the bgl contained an open reading frame of 1314 bp encoding a protein of 446 amino acid residues, and the product belonged to the glycoside hydrolase family 1 with the canonical glyco ...
DOI:
10.1007/s00253-015-6619-9

60. Soil peroxidase regulates organic matter decomposition through improving the accessibility of reducing sugars and amino acids

Author:
Lei Tian; Wei Shi
Source:
Biology and fertility of soils 2014 v.50 no.5 pp. 785-794
ISSN:
0178-2762
Subject:
humus; correlation; mineralization; proteins; reducing sugars; bioavailability; soil properties; microorganisms; arable soils; amino acids; endo-1,4-beta-glucanase; peroxidase; beta-glucosidase; phenolic compounds
Abstract:
... Peroxidase activity can be an important soil property for predicting soil organic matter dynamics, but peroxidase control on organic matter degradation has not yet been fully understood. We hypothesized that peroxidase could enhance soil C and N mineralization via improving the bioavailability of reducing sugars and amino acids. First, an arable soil (~13 g dry weight equivalent) was amended with ...
DOI:
10.1007/s00374-014-0903-1

61. Vegetation type affects soil enzyme activities and microbial functional diversity following re-vegetation of a severely eroded red soil in sub-tropical China

Author:
Rui Yin; Huan Deng; Hui-li Wang; Bin Zhang
Source:
Catena 2014 v.115 pp. 96-103
ISSN:
0341-8162
Subject:
B horizons; Cinnamomum camphora; Lespedeza; Pinus massoniana; amines; amino acids; beta-glucosidase; carbohydrates; carbon; carboxylic acids; catechol oxidase; community structure; endo-1,4-beta-glucanase; enzyme activity; eroded soils; functional diversity; microbial activity; microbial biomass; organic horizons; phenolic compounds; physicochemical properties; planting; polymers; soil enzymes; soil microorganisms; soil sampling; urease; vegetation types; China
Abstract:
... The objectives of this study were to evaluate the restorative effects of re-vegetation and vegetation type on soil enzyme activities and microbial functional diversity of eroded red soil. Soil samples were collected by horizon from eroded soils that had been restored for 18years with Cinnamomum camphora, Pinus massoniana or Lespedeza bicolor. Un-eroded soils planted with these vegetation types and ...
DOI:
10.1016/j.catena.2013.11.015

62. Cloning and characterization of a new broadspecific β-glucosidase from Lactococcus sp. FSJ4

Author:
Shujun Fang; Jie Chang; Yong Seok Lee; Weiliang Guo; Yong Lark Choi; Yongcan Zhou
Source:
World journal of microbiology & biotechnology 2014 v.30 no.1 pp. 213-223
ISSN:
0959-3993
Subject:
Lactococcus; amino acids; beta-glucosidase; catalytic activity; enzyme activity; galactose; genes; glucose; glycosides; models; open reading frames; pH; polyacrylamide gel electrophoresis; substrate specificity; xylose
Abstract:
... A β-glucosidase gene bglX was cloned from Lactococcus sp. FSJ4 by the method of shotgun. The bglX open reading frame consisted of 1,437 bp, encoding 478 amino acids. SDS-PAGE showed a recombinant bglX monomer of 54 kDa. Substrate specificity study revealed that the enzyme exhibited multifunctional catalysis activity against pNPG, pNPX and pNPGal. This enzyme shows higher activity against aryl glyc ...
DOI:
10.1007/s11274-013-1444-8
PubMed:
23892562

63. Cloning and expression of A. oryzae β-glucosidase in Pichia pastoris

Author:
Zizhong Tang; Shan Liu; Haijun Jing; Rong Sun; Moyang Liu; Hui Chen; Qi Wu; Xueyi Han
Source:
Molecular biology reports 2014 v.41 no.11 pp. 7567-7573
ISSN:
0301-4851
Subject:
Aspergillus oryzae; Komagataella pastoris; complementary DNA; glycoproteins; open reading frames; secretion; gene expression regulation; nucleotides; introns; amino acids; pH; molecular cloning; catalytic activity; beta-glucosidase
Abstract:
... A β-glucosidase gene (bgl) from Aspergillus oryzae GIF-10 was cloned, sequenced and expressed. Its full-length DNA sequence was 2,903 bp and included three introns. The full-length cDNA sequence contained an open reading frame of 2,586 nucleotides, encoding 862 amino acids with a potential secretion signal. The A. oryzae GIF-10 bgl was functionally expressed in Pichia pastoris. After 7-day inducti ...
DOI:
10.1007/s11033-014-3644-1
PubMed:
25123895

64. Cloning and expression of β-1, 4-endoglucanase gene from Bacillus subtilis isolated from soil long term irrigated with effluents of paper and pulp mill

Author:
Sangeeta Pandey; Jyoti Kushwah; Rameshwar Tiwari; Ram Kumar; Vishal Singh Somvanshi; Lata Nain; Anil Kumar Saxena
Source:
Microbiological research 2014 v.169 pp. 693-698
ISSN:
0944-5013
Subject:
Bacillus subtilis; Escherichia coli; amino acids; beta-glucosidase; endo-1,4-beta-glucanase; enzyme activity; gene overexpression; genes; molecular cloning; molecular weight; nucleotides; open reading frames; pH; polyacrylamide gel electrophoresis; pulp and paper mill effluents; sequence analysis; soil; temperature
Abstract:
... A strain of Bacillus subtilis IARI-SP-1 isolated from soil long term irrigated with effluents of paper and pulp mill showed high β-1, 4-endoglucanase (2.5IU/ml) but low activity of β-1, 4-exoglucanase (0.8IU/ml) and β-glucosidase (0.084IU/ml). The β-1, 4-endoglucanase gene of IARI-SP-1 was amplified using degenerate primers designed based on sequences already available in NCBI GenBank. A full leng ...
DOI:
10.1016/j.micres.2014.02.006

65. Enhanced Catalytic Efficiency in Quercetin-4′-glucoside Hydrolysis of Thermotoga maritima β-Glucosidase A by Site-Directed Mutagenesis

Author:
Huihui Sun; Yemin Xue; Yufei Lin
Source:
Journal of agricultural and food chemistry 2014 v.62 no.28 pp. 6763-6770
ISSN:
0021-8561
Subject:
Thermoanaerobacter ethanolicus; Thermotoga maritima; amino acids; beta-glucosidase; catalytic activity; enzymatic hydrolysis; enzyme activity; glucosides; hydrolysis; isoflavones; models; mutants; pH; quercetin; sequence analysis; site-directed mutagenesis; substrate specificity; temperature
Abstract:
... Te-BglA and Tm-BglA are glycoside hydrolase family 1 β-glucosidases from Thermoanaerobacter ethanolicus JW200 and Thermotoga maritima, respectively, with 53% sequence identity. However, Te-BglA could more effectively hydrolyze isoflavone glucosides to their aglycones than could Tm-BglA, possibly due to the difference in amino acid residues around their glycone binding pockets. Site-directed mutag ...
DOI:
10.1021/jf501932v

66. In vitro evaluation of the safety and probiotic properties of Lactobacilli isolated from chicken and calves

Author:
Dobroslava Bujnakova; Eva Strakova; Vladimir Kmet
Source:
Anaerobe 2014 v.29 pp. 118-127
ISSN:
1075-9964
Subject:
Escherichia coli; Klebsiella; Lactobacillus agilis; Lactobacillus amylovorus; Lactobacillus murinus; Lactobacillus reuteri; Lactobacillus salivarius; acid tolerance; agmatine; amino acids; beta-glucosidase; beta-glucuronidase; bile resistance; biofilm; calves; chickens; competitive exclusion; desorption; enzyme activity; fluorescence in situ hybridization; gastrointestinal system; genes; gentamicin; gentian violet; histamine; histidine decarboxylase; in vitro studies; kanamycin; mass spectrometry; ornithine decarboxylase; pH; pathogens; polymerase chain reaction; probiotics; putrescine; safety assessment; screening; streptomycin; tyramine; tyrosine decarboxylase
Abstract:
... A total of 73 chicken and calves isolates were diagnosed using matrix-assisted laser desorption ionization-time-of flight mass spectrometry (Maldi-Tof MS). After a preliminary subtractive screening based on the high acid tolerance at pH 2.5 and bile resistance at 0.3% oxgall, twenty isolates belonging to the species Lactobacillus salivarius, Lactobacillus agilis, Lactobacillus reuteri, Lactobacill ...
DOI:
10.1016/j.anaerobe.2013.10.009

67. Residues affecting hydrolysis of soy isoflavone glycosides, stability and catalytic properties of Thermotoga maritima β-glucosidase

Author:
Y. M. Xue; X. F. Song; C. Y. Xu; H. H. Sun; Z. G. Cao
Source:
Applied biochemistry and microbiology 2013 v.49 no.5 pp. 464-472
ISSN:
0003-6838
Subject:
Escherichia coli; Thermotoga maritima; amino acids; beta-glucosidase; catalytic activity; daidzin; glycosides; hydrolysis; mutants; mutation; temperature; thermal stability
Abstract:
... The thermostable β-glucosidase A (TmBglA) from Thermotoga maritima is a promising biocatalyst for production of isoflavone aglycones. Use of enzymes with high specificity for soy isoflavone conjugates is however essential for efficient hydrolysis. The effect of the amino acids located in the aglycone binding pocket with non-conserved residues between specificity groups in family 1 glycoside hydrol ...
DOI:
10.1134/S0003683813050153

68. Separating cellular metabolism from exoenzyme activity in soil organic matter decomposition

Author:
Joseph C. Blankinship; Caryl A. Becerra; Sean M. Schaeffer; Joshua P. Schimel
Source:
Soil biology & biochemistry 2014 v.71 pp. 68-75
ISSN:
0038-0717
Subject:
alpha-glucosidase; amino acids; autoclaving; beta-glucosidase; biochemical pathways; carbon dioxide; catalysts; cell viability; cellulose 1,4-beta-cellobiosidase; chloroform; decarboxylation; depolymerization; fumigation; glucose; grassland soils; greenhouse gas emissions; irradiation; metabolism; regrowth; soil organic matter
Abstract:
... Soil organic matter (SOM) decomposes both inside and outside of cells. Cellular metabolism and extracellular depolymerization normally operate simultaneously in soil but are difficult to separate in practice. To learn more about the extracellular component of SOM decomposition, we sterilized a semiarid annual grassland soil to inhibit cellular metabolism, and then assayed cell viability, exoenzyme ...
DOI:
10.1016/j.soilbio.2014.01.010

69. dl-2 Aminobutyric acid and calliterpinone are the potential stimulators of Trichoderma cellulase activities

Author:
Mahesh Chandra; Neelam S. Sangwan; Hirdesh Kumar; Anil K. Singh; Alok Kalra
Source:
Biomass and bioenergy 2014 v.62 pp. 212-217
ISSN:
0961-9534
Subject:
Trichoderma citrinoviride; amino acids; beta-glucosidase; diterpenoids; endo-1,4-beta-glucanase; enzyme activity; fermentation; fungi; isomers; jasmonic acid; molecular weight; mutants; plant growth substances
Abstract:
... Trichoderma citrinoviride EB-104 has recently been developed as a hyper β-glucosidase producing mutant strain. In the present study, efforts were made to further enhance its enzyme titers using low molecular weight stimulators viz., calliterpinone (a natural plant diterpenoid), dl-2-aminobutyric acid (isomer of an amino acid, aminobutyric acid) and jasmonic acid (a natural plant growth regulator). ...
DOI:
10.1016/j.biombioe.2014.01.026

70. Biochemical properties of a novel glycoside hydrolase family 1 β-glucosidase (PtBglu1) from Paecilomyces thermophila expressed in Pichia pastoris

Author:
Shaoqing Yang; Chengwei Hua; Qiaojuan Yan; Yinan Li; Zhengqiang Jiang
Source:
Carbohydrate polymers 2013 v.92 no.1 pp. 784-791
ISSN:
0144-8617
Subject:
substrate specificity; Komagataella pastoris; fermenters; glycoproteins; open reading frames; nucleotides; cellobiose; amino acids; Paecilomyces; genes; pH; thermophilic fungi; molecular weight; catalytic activity; glycosides; beta-glucosidase; industrial applications; gentiobiose
Abstract:
... A novel β-glucosidase gene (PtBglu1) from the thermophilic fungus, Paecilomyces thermophila, was cloned and expressed in Pichia pastoris. PtBglu1 contained an open reading frame of 1440-bp nucleotides and encoded a protein of 479 amino acids which showed significant similarity to other fungal β-glucosidases from glycoside hydrolase (GH) family 1. The recombinant β-glucosidase (PtBglu1) was secrete ...
DOI:
10.1016/j.carbpol.2012.09.086

71. Biotransformation of ginsenosides Re and Rg1 into ginsenosides Rg2 and Rh1 by recombinant β-glucosidase

Author:
Lin-Hu Quan; Jin-Woo Min; Subramaniyam Sathiyamoorthy; Dong-Uk Yang; Yeon-Ju Kim; Deok-Chun Yang
Source:
Biotechnology letters 2012 v.34 no.5 pp. 913-917
ISSN:
0141-5492
Subject:
amino acids; beta-glucosidase; biotransformation; genes; glucose; sodium phosphate
Abstract:
... Ginsenosides Re and Rg1 were transformed by recombinant β-glucosidase (Bgp1) to ginsenosides Rg2 and Rh1, respectively. The bgp1 gene consists of 2,496 bp encoding 831 amino acids which have homology to the glycosyl hydrolase families 3 protein domain. Using 0.1 mg enzyme ml−1 in 20 mM sodium phosphate buffer at 37°C and pH 7.0, the glucose moiety attached to the C-20 position of ginsenosides Re a ...
DOI:
10.1007/s10529-012-0849-z
PubMed:
22261865

72. Characterisation of a novel Bacillus sp. SJ-10 β-1,3–1,4-glucanase isolated from jeotgal, a traditional Korean fermented fish

Author:
Yu-Ri Kim; Eun-Young Kim; Jong Min Lee; Joong Kyun Kim; In-Soo Kong
Source:
Bioprocess and biosystems engineering 2013 v.36 no.6 pp. 721-727
ISSN:
1615-7591
Subject:
Bacillus (bacteria); Escherichia coli; affinity chromatography; amino acids; barley; beta-glucanase; beta-glucans; beta-glucosidase; bioprocessing; engineering; enzymatic treatment; fermented fish; gene banks; genes; glucose; molecular cloning; open reading frames; pH; recombinant proteins; substrate specificity; temperature; thin layer chromatography; Korean Peninsula
Abstract:
... A novel β-1,3–1,4-glucanase gene was identified in Bacillus sp. SJ-10 (KCCM 90078) isolated from jeotgal, a traditional Korean fermented fish. We analysed the β-1,3–1,4-glucanase gene sequence and examined the recombinant enzyme. The open reading frame of the gene encoded 244 amino acids. The sequence was not identical to any β-glucanases deposited in GenBank. The gene was cloned into pET22b(+) an ...
DOI:
10.1007/s00449-013-0896-4

73. Defensive role of Gossypium hirsutum L. anti-oxidative enzymes and phenolic acids in response to Spodoptera litura F. feeding

Author:
Pathipati Usha Rani; Sambangi Pratyusha
Source:
Journal of Asia-Pacific entomology 2013 v.16 no.2 pp. 131-136
ISSN:
1226-8615
Subject:
Gossypium hirsutum; Spodoptera litura; amino acids; ammonia; assays; beta-glucosidase; caffeic acid; carbohydrates; catalase; catechin; catechol oxidase; cotton; digestive system; esterases; gallic acid; glutathione transferase; herbivores; high performance liquid chromatography; insects; laboratory experimentation; metabolites; peroxidase; pests; phenols; proteins; superoxide dismutase; toxicity
Abstract:
... The responses of the cotton plant, Gossypium hirsutum L. to herbivory by Spodoptera litura F. was studied in various laboratory experiments as a measure to understand the defense strategies of certain plant metabolites. Insect feeding damage enhanced the concentration of total phenol content and proteins, whereas amount of carbohydrates and amino acids were reduced. The experiments on estimation o ...
DOI:
10.1016/j.aspen.2013.01.001

74. Direct L-lysine production from cellobiose by Corynebacterium glutamicum displaying beta-glucosidase on its cell surface

Author:
Noriko Adachi; Chihiro Takahashi; Naoko Ono-Murota; Rie Yamaguchi; Tsutomu Tanaka; Akihiko Kondo
Source:
Applied microbiology and biotechnology 2013 v.97 no.16 pp. 7165-7172
ISSN:
0175-7598
Subject:
Corynebacterium glutamicum; absorbance; amino acids; beta-glucosidase; carbon; cellobiose; fermentation; glucose; porins; screening
Abstract:
... We constructed beta-glucosidase (BGL)-displaying Corynebacterium glutamicum, and direct L-lysine fermentation from cellobiose was demonstrated. After screening active BGLs, Sde1394, which is a BGL from Saccharophagus degradans, was successfully displayed on the C. glutamicum cell surface using porin as an anchor protein, and cellobiose was directly assimilated as a carbon source. The optical densi ...
DOI:
10.1007/s00253-013-5009-4
PubMed:
23749228

75. Exchanging a single amino acid residue generates or weakens a +2 cellooligosaccharide binding subsite in rice β-glucosidases

Author:
Sompong Sansenya; Janjira Maneesan; James R. Ketudat Cairns
Source:
Carbohydrate research 2012 v.351 no. pp. 130-133
ISSN:
0008-6215
Subject:
X-ray diffraction; amino acids; beta-glucosidase; glycosides; hydrogen bonding; hydrolysis; mutation; oligosaccharides; polymerization; rice
Abstract:
... Os3BGlu6, Os3BGlu7, and Os4BGlu12 are rice glycoside hydrolase family 1 β-glucosidases, the structures of which have been solved by X-ray crystallography. In complex structures, Os3BGlu7 residue Asn245 hydrogen bonds to the second sugar in the +1 subsite for laminaribiose and the third sugar in the +2 subsite for cellotetraose and cellopentaose. The corresponding Os3BGlu6 residue, Met251, appears ...
DOI:
10.1016/j.carres.2012.01.010

76. Expression and characterization of GH3 β-Glucosidase from Aspergillus niger NL-1 with high specific activity, glucose inhibition and solvent tolerance

Author:
Linguo Zhao; Tanche Zhou; Xun Li; Song Fan; Lijin You
Source:
Microbiology 2013 v.82 no.3 pp. 356-363
ISSN:
0026-2617
Subject:
fermentation; Aspergillus niger; Komagataella pastoris; methanol; glucose; open reading frames; polyacrylamide gel electrophoresis; ultrafiltration; EDTA (chelating agent); cellobiose; amino acids; genes; pH; solvents; metal ions; glycosides; beta-glucosidase; industrial applications
Abstract:
... A β-glucosidase gene bglI from Aspergillus niger NL-1 was cloned and expressed in Pichia pastoris. The bglI gene consists of a 2583 bp open reading frame encoding 861 amino acids; the enzyme was classified into glycoside hydrolases 3. To improve the expression level of recombinant BGL in P. pastoris, fermentation conditions were optimized by the single-factor experiments. The optimal fermentation ...
DOI:
10.1134/S0026261713030181

77. Influence of seasons and land-use practices on soil microbial activity and metabolic diversity in the “Montado ecosystem”

Author:
Dalila Costa; Helena Freitas; José Paulo Sousa
Source:
European journal of soil biology 2013 v.59 pp. 22-30
ISSN:
1164-5563
Subject:
Quercus ilex subsp. rotundifolia; Quercus suber; acid phosphatase; amino acids; autumn; beta-glucosidase; cork; crops; ecosystems; fallow; land use; microbial activity; mineralization; nitrification; organic matter; pastures; polymers; seasonal variation; soil microorganisms; soil water content; spring; urease; wet season; winter
Abstract:
... The “Montado ecosystem” is important both economically and ecologically; this ecosystem is dominated by cork and holm oak trees (Quercus suber L. and Quercus rotundifolia Lam. respectively) combined with a rotation of crops/fallow/pastures. Diverse management strategies, deviating from the sustainable use of the ecosystem, have been implemented, from which arise some extreme situations of over-use ...
DOI:
10.1016/j.ejsobi.2013.08.003

78. Investigation of the functional role of aldose 1-epimerase in engineered cellobiose utilization

Author:
Sijin Li; Suk-Jin Ha; Hee Jin Kim; Jonathan M. Galazka; Jamie H.D. Cate; Yong-Su Jin; Huimin Zhao
Source:
Journal of biotechnology 2013 v.168 pp. 1-6
ISSN:
0168-1656
Subject:
Neurospora crassa; Saccharomyces cerevisiae; aldose 1-epimerase; amino acids; beta-glucosidase; biotechnology; cell growth; cellobiose; fermentation; xylose
Abstract:
... Functional expression of a cellodextrin transporter and an intracellular β-glucosidase from Neurospora crassa in Saccharomyces cerevisiae enables simultaneous co-fermentation of cellobiose and non-glucose sugars such as xylose. Here we investigate the functional role of aldose 1-epimerase (AEP) in engineered cellobiose utilization. One AEP (Gal10) and two putative AEPs (Yhr210c and Ynr071c sharing ...
DOI:
10.1016/j.jbiotec.2013.08.003

79. Molecular and biochemical characterization of a cyanogenic β-glucosidase in the inner bark tissues of rubber tree (Hevea brasiliensis Muell. Arg.)

Author:
Wei-Min Tian; Hua Zhang; Shu-Guang Yang; Min-Jing Shi; Xu-Chu Wang; Long-Jun Dai; Yue-Yi Chen
Source:
Journal of plant physiology 2013 v.170 no.8 pp. 723-730
ISSN:
0176-1617
Subject:
Hevea brasiliensis; Manihot esculenta; amino acids; bark; beta-glucosidase; biosynthesis; cassava; complementary DNA; gene expression regulation; genes; latex; leaves; nitrogen; open reading frames; proteins; rubber; vacuoles
Abstract:
... Tapping causes the loss of large amounts of latex from laticifers and subsequently enhances latex regeneration, a high carbon- and nitrogen-cost activity in rubber tree. It is suggested that a 67kDa protein associated with protein-storing cells in the inner bark tissues of rubber tree plays an important role in meeting the nitrogen demand for latex regeneration. Here, the 67kDa protein was further ...
DOI:
10.1016/j.jplph.2012.12.019
PubMed:
23510639

80. Prediction of Optimal pH in Hydrolytic Reaction of Beta-glucosidase

Author:
Shaomin Yan; Guang Wu
Source:
Applied biochemistry and biotechnology 2013 v.169 no.6 pp. 1884-1894
ISSN:
0273-2289
Subject:
amino acids; beta-glucosidase; bioenergy industry; enzymatic reactions; neural networks; pH; prediction; probability distribution
Abstract:
... This is the continuation of our studies to use very basic information on enzyme to predict optimal reaction parameters in enzymatic reactions because the gap between available enzyme sequences and their available reaction parameters is widening. In this study, 23 features selected from 540 plus features of individual amino acid as well as a feature combined whole protein information were screened ...
DOI:
10.1007/s12010-013-0103-8
PubMed:
23344943

81. Bioconversion of ginsenosides Rb₁, Rb₂, Rc and Rd by novel β-glucosidase hydrolyzing outer 3-O glycoside from Sphingomonas sp. 2F2: Cloning, expression, and enzyme characterization

Author:
Liang Wang; Qing-Mei Liu; Bong-Hyun Sung; Dong-Shan An; Hyung-Gwan Lee; Song-Gun Kim; Sun-Chang Kim; Sung-Taik Lee; Wan-Taek Im
Source:
Journal of biotechnology 2011 v.156 no.2 pp. 125-133
ISSN:
0168-1656
Subject:
Escherichia coli; Panax; Sphingomonas; amino acids; beta-glucosidase; biotechnology; biotransformation; chromatography; enzymatic reactions; genes; ginsenosides; glucose; molecular weight; pH
Abstract:
... A new β-glucosidase gene (bglSp) was cloned from the ginsenoside converting Sphingomonas sp. strain 2F2 isolated from the ginseng cultivating filed. The bglSp consisted of 1344bp (447 amino acid residues) with a predicted molecular mass of 49,399Da. A BLAST search using the bglSp sequence revealed significant homology to that of glycoside hydrolase superfamily 1. This enzyme was overexpressed in ...
DOI:
10.1016/j.jbiotec.2011.07.024

82. Characterization of a novel thermostable β-glucosidase from a metagenomic library of termite gut

Author:
Qianfu Wang; Changli Qian; Xiao-Zhou Zhang; Nin Liu; Xing Yan; Zhihua Zhou
Source:
Enzyme and microbial technology 2012 v.51 no.6-7 pp. 319-324
ISSN:
0141-0229
Subject:
Acidothermus cellulolyticus; Escherichia coli; Isoptera; Trichoderma reesei; active sites; amino acids; beta-glucosidase; cellulose; cellulose 1,4-beta-cellobiosidase; digestive system; endo-1,4-beta-glucanase; fungi; genes; genomic libraries; glycosides; half life; industrial applications; molecular weight; pH; polyacrylamide gel electrophoresis; polypeptides; synergism; temperature; thermal stability
Abstract:
... A novel β-glucosidase-encoding gene, bgl-gs1, which was identified from a positive fosmid clone in a metagenomic library of the gut of Globitermes sulphureus, encodes a 455 amino acid polypeptide that contains a catalytic domain belonging to glycoside hydrolase family 1 (GH1). It was expressed in Escherichia coli BL21 (DE3) and the expression product showed a molecular mass of ∼51.7kDa by SDS-PAGE ...
DOI:
10.1016/j.enzmictec.2012.07.015

83. Cloning and biochemical analysis of β-glucoside utilization (bgl) operon without phosphotransferase system in Pectobacterium carotovorum subsp. carotovorum LY34

Author:
Chang Long An; Min Keun Kim; Tae Ho Kang; Jungho Kim; Hoon Kim; Han Dae Yun
Source:
Microbiological research 2012 v.167 no.8 pp. 461-469
ISSN:
0944-5013
Subject:
DNA fragmentation; Pectobacterium carotovorum subsp. carotovorum; amino acids; bacteria; beta-glucosidase; genomic libraries; magnesium; metabolism; open reading frames; operon; pH; promoter regions; proteins; screening; sequence analysis; signal peptide; site-directed mutagenesis; sugars; terminal repeat sequences; transcription (genetics); transportation
Abstract:
... β-Glucosidases are widespread in bacteria and involved in the metabolism of various carbohydrate substrates. Studying of β-glucoside utilization (bgl) operons on operon of Pectobacterium carotovorum subsp. carotovorum LY34 (Pcc LY34) will help us understanding how β-glucoside utilization (bgl) operon can cooperate with other systems in bacterium caused soft-rot disease. Pcc LY34 causes soft-rot di ...
DOI:
10.1016/j.micres.2012.03.004

84. Cloning and characterization of a novel cellobiase gene, cba3, encoding the first known β-glucosidase of glycoside hydrolase family 1 of Cellulomonas biazotea

Author:
Anthony K.N. Chan; Yule Y. Wang; K.L. Ng; Zhibiao Fu; W.K.R. Wong
Source:
Gene 2012 v.493 no.1 pp. 52-61
ISSN:
0378-1119
Subject:
Cellulomonas biazotea; Escherichia coli; amino acids; beta-glucosidase; biomass; carbon; catalytic activity; cellobiose; enzyme activity; genes; glycosides; hydrolysis; signal peptide
Abstract:
... A novel cellobiase gene, designated cba3, was cloned from Cellulomonas biazotea. Although cellobiase genes of C. biazotea were previously cloned, published and/or patented, they encoded β-glucosidases all belonging to glycoside hydrolase family 3 (GH3); the new Cba3 cellobiase was identified to be a glycoside hydrolase family 1 (GH1) member, which represents the first discovered GH1 β-glucosidase ...
DOI:
10.1016/j.gene.2011.11.027
PubMed:
22138482

85. Cloning and characterization of a putative β-glucosidase (NfBGL595) from Neosartorya fischeri

Author:
Priyadharshini Ramachandran; Manish Kumar Tiwari; Raushan Kumar Singh; Jung-Rim Haw; Marimuthu Jeya; Jung-Kul Lee
Source:
Process biochemistry 2012 v.47 no.1 pp. 99-105
ISSN:
1359-5113
Subject:
Aspergillus fischeri; DNA; Escherichia coli; Trichoderma reesei; X-ray diffraction; amino acids; beta-glucosidase; catalytic activity; cellobiose; enzyme activity; gene overexpression; genes; glycosides; models; molecular dynamics; open reading frames; pH; polypeptides; sequence analysis; substrate specificity; temperature
Abstract:
... Whole genome sequence of Neosartorya fischeri NRRL181 revealed four putative GH1 β-glucosidases (BGLs). One BGL, NfBGL595 was successfully expressed and characterized. DNA sequence analysis revealed an open reading frame of 1590bp, encoding a polypeptide of 529 amino acid residues. The gene was cloned in pET28a and overexpressed in Escherichia coli. The purified recombinant BGL showed high levels ...
DOI:
10.1016/j.procbio.2011.10.015

86. Enzymatic Biotransformation of Ginsenoside Rb1 to Compound K by Recombinant β-Glucosidase from Microbacterium esteraromaticum

Author:
Quan Lin-Hu; Min Jin-Woo; Jin Yan; Wang Chao; Kim Yeon-Ju; Yang Deok-Chun
Source:
Journal of agricultural and food chemistry 2012 v.60 no.14 pp. 3776-3781
ISSN:
1520-5118
Subject:
Microbacterium esteraromaticum; Panax; amino acids; beta-glucosidase; biotransformation; genes; glucose; hydrolysis; molecular weight; pH; polyacrylamide gel electrophoresis; sodium phosphate
Abstract:
... We cloned and characterized a β-glucosidase (bgp3) gene from Microbacterium esteraromaticum isolated from ginseng field. The bgp3 gene consists of 2,271 bp encoding 756 amino acids which have homology to the glycosyl hydrolase family 3 protein domain. The molecular mass of purified Bgp3 was 80 kDa, as determined by SDS–PAGE. The enzyme (Bgp3) catalyzed the conversion of ginsenoside Rb1 to the more ...
DOI:
10.1021/jf300186a
PubMed:
22428991

87. Focused directed evolution of β-glucosidases: theoretical versus real effectiveness of a minimal working setup and simple robust screening

Author:
Pavel Mazura; Tomáš Filipi; Přemysl Souček; Břetislav Brzobohatý
Source:
Carbohydrate research 2011 v.346 no.2 pp. 238-242
ISSN:
0008-6215
Subject:
active sites; amino acid sequences; amino acids; beta-glucosidase; chemical derivatives; corn; enzyme activity; mutants; phenotype; phenotypic variation; proteins; site-directed mutagenesis; structure-activity relationships
Abstract:
... Here we present an optimized procedure to generate amino acid variations at specific site(s) of proteins, followed by a simple one-step screen for mutants with the desired β-glucosidase activity. The procedure was evaluated by introducing sequence variation into a codon specifying a non-functional variant of the catalytic nucleophile (E401) of the maize β-glucosidase Zm-p60.1. Observed and theoret ...
DOI:
10.1016/j.carres.2010.08.016

88. Genome-wide analysis of the beta-glucosidase gene family in maize (Zea mays L. var B73)

Author:
Gracia Gómez-Anduro; Esther Adriana Ceniceros-Ojeda; Luz Edith Casados-Vázquez; Christelle Bencivenni; Arturo Sierra-Beltrán; Bernardo Murillo-Amador; Axel Tiessen
Source:
Plant molecular biology 2011 v.77 no.1-2 pp. 159-183
ISSN:
0167-4412
Subject:
sequence alignment; phylogeny; promoter regions; gene expression; amino acids; genotype; signal peptide; introns; beta-glucosidase; transcription factors; Zea mays; variance; corn; transcription (genetics); hydrolysis; genetic variance; isozymes; exons; microarray technology; reverse transcriptase polymerase chain reaction; environmental factors; gene duplication; binding sites
Abstract:
... The hydrolysis of beta-D: -glucosidic bonds which is required for the liberation of many physiologically important compounds is catalyzed by the enzyme beta-glucosidase (BGLU, EC 3.2.1.21). BGLUs are implicated in several processes in plants, such as the timely response to biotic and abiotic stresses through activation of phytohormones and defense compounds. We identified 26 BGLU isozymes in the g ...
DOI:
10.1007/s11103-011-9800-2

89. Identifying and characterizing the most significant β-glucosidase of the novel species Aspergillus saccharolyticus

Author:
Annette Sørensen; Birgitte K. Ahring; Mette Lübeck; Wimal Ubhayasekera; Kenneth S. Bruno; David E. Culley; Peter S. Lübeck
Source:
Canadian journal of microbiology 2012 v.58 no.9 pp. 1035-1046
ISSN:
1480-3275
Subject:
Aspergillus aculeatus; Aspergillus niger; Trichoderma reesei; amino acids; beta-glucosidase; cellobiose; culture media; enzyme kinetics; fungi; genes; glycosides; half life; ion exchange chromatography; mass spectrometry; models; polyacrylamide gel electrophoresis; polymerase chain reaction; polypeptides; thermal stability
Abstract:
... The newly discovered fungal species Aspergillus saccharolyticus was found to produce a culture broth rich in β-glucosidase activity. In this present work, the main β-glucosidase of A. saccharolyticus responsible for the efficient hydrolytic activity was identified, isolated, and characterized. Ion exchange chromatography was used to fractionate the culture broth, yielding fractions with high β-glu ...
DOI:
10.1139/w2012-076

90. A new β-glucosidase gene from the zygomycete fungus Rhizomucor miehei

Author:
Takó, Miklós; Tóth, Adél; Nagy, László G.; Krisch, Judit; Vágvölgyi, Csaba; Papp, Tamás
Source:
Antonie van Leeuwenhoek 2010 v.97 no.1 pp. 1-10
ISSN:
0003-6072
Subject:
Mucor; proteins; Rhizomucor miehei; plasmids; amino acids; active sites; introns; sequence homology; beta-glucosidase; fungi
Abstract:
... In this study, a β-glucosidase coding gene (bgl) of the zygomycete fungus Rhizomucor miehei has been cloned and characterized. The gene comprises a total of 2,826 bp including the coding sequence of a 717 amino acids length putative protein and 10 introns dispersed in the whole coding region. The putative N-and C-terminal catalytic domains (aa 68 to aa 274 and aa 358-601, respectively) were identi ...
DOI:
10.1007/s10482-009-9382-z

91. Chemical composition of dissolved organic matter in agroecosystems: Correlations with soil enzyme activity and carbon and nitrogen mineralization

Author:
Tian, Lei; Dell, Emily; Shi, Wei
Source:
Applied soil ecology 2010 v.46 no.3 pp. 426-435
ISSN:
0929-1393
Subject:
agricultural soils; soil organic carbon; dissolved organic carbon; soil ecology; reducing sugars; amino acids; molecular weight; peroxidase; beta-glucosidase; agroecosystems; mineralization; catechol oxidase; enzyme activity; nitrogen; soil enzymes; phenolic compounds
Abstract:
... Soil enzyme-catalyzed depolymerization of organic matter results in the production of low molecular weight and dissolved organic compounds. This fraction of soil organic matter is the immediate energy, carbon and other nutrient substrates for microbial catabolic pathways and thus likely plays an important role in soil processes. The purpose of this study was to elucidate interrelationships among d ...
DOI:
10.1016/j.apsoil.2010.09.007

92. Organic matter availability during pre- and post-drought periods in a Mediterranean stream

Author:
Ylla, Irene; Sanpera-Calbet, Isis; Vázquez, Eusebi; Romaní, Anna M.; Muñoz, Isabel; Butturini, Andrea; Sabater, Sergi
Source:
Hydrobiologia 2010 v.657 no.1 pp. 217-232
ISSN:
0018-8158
Subject:
amino acid composition; amino acids; bacterial communities; beta-glucosidase; biofilm; climate change; drought; drying; ecosystems; enzyme activity; floods; heterotrophs; leaves; leucyl aminopeptidase; lipid content; microorganisms; organic matter; peptides; polysaccharides; resource management; rivers; rocks; sand; stream channels; water content; water flow
Abstract:
... Mediterranean streams are characterized by water flow changes caused by floods and droughts. When intermittency occurs in river ecosystems, hydrologic connectivity is interrupted and this affects benthic, hyporheic and flowing water compartments. Organic matter use and transport can be particularly affected during the transition from wet to dry and dry to wet conditions. In order to characterize t ...
DOI:
10.1007/s10750-010-0193-z

93. Screening and characterization of a cellulase with endocellulase and exocellulase activity from yak rumen metagenome

Author:
Lei Bao; Qiang Huang; Lei Chang; Jungang Zhou; Hong Lu
Source:
Journal of molecular catalysis 2011 v.73 no.1-4 pp. 104-110
ISSN:
1381-1177
Subject:
rumen; lignocellulose; catalytic activity; mechanism of action; barley; glucose; amino acids; phosphates; screening; yaks; beta-glucosidase; saccharification; carboxymethylcellulose; Escherichia coli; polymerization; cellobiose; metagenomics; enzyme activity; biomass; hydrolysates; biofuels; endo-1,4-beta-glucanase
Abstract:
... Cellulose saccharification is an important process in conversion from lignocellulosic biomass to biofuels and other chemicals, and requires concerted action of endocellulase, exocellulase and β-glucosidase. Thus, it is very interesting to discover and develop multifunctional cellulase in order to convert cellulose to glucose more efficiently. Here we report an endo/exocellulase Rucel5B with 336 am ...
DOI:
10.1016/j.molcatb.2011.08.006

94. A Novel Cold-Active and Alkali-Stable β-Glucosidase Gene Isolated from the Marine Bacterium Martelella mediterranea

Author:
Mao, Xiaoxia; Hong, Yuzhi; Shao, Zongze; Zhao, Yan; Liu, Ziduo
Source:
Applied biochemistry and biotechnology 2010 v.162 no.8 pp. 2136-2148
ISSN:
0273-2289
Subject:
buffers; genomic libraries; Agrobacterium radiobacter; industrial applications; amino acids; genes; pH; temperature; enzyme activity; metal ions; beta-glucosidase; bacteria
Abstract:
... A β-glucosidase gene designated gluc3m was cloned through construction of a genomic library of Martelella mediterranea 2928. The gluc3m consisted of 2,496 bp and encoded a peptide of 832 amino acids that shared the greatest amino acid similarity (59%) with a β-glucosidase of family 3 glycoside hydrolase from Agrobacterium radiobacter K84. The optimum reaction temperature and pH of Gluc3M were 45 ° ...
DOI:
10.1007/s12010-010-8988-y
PubMed:
20503105

95. Characterization of a novel β-glucosidase-like activity from a soil metagenome

Author:
Chengjian Jiang; Gefei Ma; Shuangxi Li; Tingting Hu; Zhiqun Che; Peihong Shen; Bing Yan; Bo Wu
Source:
journal of microbiology 2009 v.47 no.5 pp. 542
ISSN:
1225-8873
Subject:
Escherichia coli; amino acid sequences; amino acids; beta-glucosidase; calcium chloride; gene overexpression; genes; genomic libraries; glucose; high performance liquid chromatography; metagenomics; molecular weight; open reading frames; pH; polypeptides; protein hydrolysates; recombinant proteins; screening; soil; soil microorganisms
Abstract:
... We report the cloning of a novel β-glucosidase-like gene by function-based screening of a metagenomic library from uncultured soil microorganisms. The gene was named bgllC and has an open reading frame of 1,443 base pairs. It encodes a 481 amino acid polypeptide with a predicted molecular mass of about 57.8 kDa. The deduced amino acid sequence did not show any homology with known β-glucosidases. T ...
DOI:
10.1007/s12275-009-0024-y

96. Characterization of β-glucosidase from a strain of Penicillium purpurogenum KJS506

Author:
Jeya, Marimuthu; Joo, Ah-Reum; Lee, Kyoung-Mi; Tiwari, Manish Kumar; Lee, Kyoung-Min; Kim, Sang-Hwan; Lee, Jung-Kul
Source:
Applied microbiology and biotechnology 2010 v.86 no.5 pp. 1473-1484
ISSN:
0175-7598
Subject:
Talaromyces purpurogenus; open reading frames; nitrogen; ribosomal DNA; polymerase chain reaction; chromatography; internal transcribed spacers; microorganisms; amino acids; carbon; corn straw; genes; pH; rice straw; temperature; molecular weight; glycosides; beta-glucosidase
Abstract:
... A novel β-glucosidase (BGL)-producing strain was isolated and identified as Penicillium purpurogenum KJS506 based on its morphology and internal transcribed spacer (ITS) rDNA gene sequence. When rice straw and corn steep powder were used as carbon and nitrogen sources, respectively, the maximal BGL activity of 12.3 U ml⁻¹, one of the highest levels among BGL-producing microorganisms was observed. ...
DOI:
10.1007/s00253-009-2395-8

97. Characterization and application of recombinant β-glucosidase (BglH) from Bacillus licheniformis KCTC 1918

Author:
In Seong Choi; Seung Gon Wi; Se Ra Jung; Darshan H. Patel; Hyeun-Jong Bae
Source:
Journal of wood science 2009 v.55 no.5 pp. 329-334
ISSN:
1435-0211
Subject:
Bacillus licheniformis; adverse effects; amino acids; beta-glucosidase; calcium; cellobiose; cellulose; copper; enzyme activity; gels; glucose; glycerol; hydrolysis; magnesium; manganese; mercury; molecular weight; pH; polyacrylamide gel electrophoresis; sodium dodecyl sulfate; soil bacteria; structural genes; urea; wood
Abstract:
... β-Glucosidase (β-1,4-D-glucoside glucohydrolase: EC.3.2.1.21) catalyzes the hydrolysis of β-glucosidic bonds between saccharides and aryl or alkyl groups. A gene encoding β-glucosidase from Bacillus licheniformis KCTC 1918, an anaerobic spore-forming soil bacterium, was cloned and characterized. The structural gene for the β-glucosidase consists of 1410 bp encoding 469 amino acid residues, and has ...
DOI:
10.1007/s10086-009-1044-2

98. Functional analysis of the aglycone-binding site of the maize β-glucosidase Zm-p60.1

Author:
Dopitová, Radka; Mazura, Pavel; Janda, Lubomír; Chaloupková, Radka; Jeřábek, Petr; Damborský, Jiří; Filipi, Tomáš; Kiran, Nagavalli S.; Brzobohatý, Břetislav
Source:
FEBS journal 2008 v.275 no.24 pp. 6123-6135
ISSN:
1742-464X
Subject:
Brassica napus; Zea mays; active sites; amino acids; beta-glucosidase; computers; consensus sequence; corn; cytokinins; enzyme activity; hydrophobicity; models; mutants; mutation; plant development; substrate specificity
Abstract:
... β-Glucosidases such as Zm-p60.1 (Zea mays) and Bgl4:1 (Brassica napus) have implicated roles in regulating plant development by releasing biologically active cytokinins from O-glucosides. A key determinant of substrate specificity in Zm-p60.1 is the F193-F200-W373-F461 cluster. However, despite sharing the same substrates, amino acids in the active sites of Zm-p60.1 and Bgl4:1 differ dramatically. ...
DOI:
10.1111/j.1742-4658.2008.06735.x

99. Chemical components of Aspergillus-type Douchi, a Chinese traditional fermented soybean product, change during the fermentation process

Author:
Zhang, Jian-Hua; Tatsumi, Eizo; Fan, Jun-Feng; Li, Li-Te
Source:
International journal of food science & technology 2007 v.42 no.3 pp. 263-268
ISSN:
0950-5423
Subject:
Aspergillus; amino acids; beta-glucosidase; chemical concentration; daidzein; daidzin; fermentation; free amino acids; genistein; genistin; nitrogen; organic acids and salts; proteinases; reducing sugars; soybean products; China
Abstract:
... Douchi, a traditional fermented soybean product that originated in China, has been consumed since ancient times as a food seasoning. The influence of fermentation on the chemical components of naturally fermented douchi and Aspergillus egypticus pure-cultured douchi was investigated. Changes in per cent and/or concentration of amino-type nitrogen, total acid, reducing sugar, organic acid, amino ac ...
DOI:
10.1111/j.1365-2621.2005.01150.x

100. Enhancement of substrate recognition ability by combinatorial mutation of β-glucosidase displayed on the yeast cell surface

Author:
Takeshi Fukuda; Michiko Kato-Murai; Tetsuya Kadonosono; Hiroshi Sahara; Yoji Hata; Shin-ichiro Suye; Mitsuyoshi Ueda
Source:
Applied microbiology and biotechnology 2007 v.76 no.5 pp. 1027-1033
ISSN:
0175-7598
Subject:
Aspergillus oryzae; amino acids; beta-glucosidase; mutants; site-directed mutagenesis; yeasts
Abstract:
... Recently, in family 3 β-glucosidase (BGL), the catalytically important Asp nucleophile has been identified in the SDW segment of the SDWG sequence by site-directed mutagenesis. However, the details about the roles of each amino acid residue of the SDWG sequence have not been investigated. W293 of the SDW segment, which is the residue next to the nucleophile (D292) in family 3 BGL, is very importan ...
DOI:
10.1007/s00253-007-1070-1