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... Dark uptake of ³ ⁵SO₄ was investigated as a possible method for estimating bacterial production rates in Castle Lake, California. Assuming a fixed cellular sulfur; carbon ratio, bacterial productivities determined in this manner ranged from 2.5 to 28 mg C°m.— ³°day— ¹ in 48 in situ measurements in the summer of 1972. Average production rates increased throughout the sampling period and corresponde ...
... Azotobacter vinelandii strain ATCC 12837 and A. chroococcum strain H23 (CECT 4435) were able to grow on N‐free or NH₄Cl‐amended chemically‐defined (Burk's) media, with protocatechuic acid (1–2 mmol l⁻¹) or sodium p‐hydroxybenzoate (1–10 mmol l⁻¹) as sole carbon (C) sources. At a concentration of 2 mmol l⁻¹, both substrates supported nitrogen fixation (acetylene reduction assay) at similar or highe ...
... The acidulation effects of the prolonged use of nitrogenous fertilizers and lime application on nitrifying bacteria and nitrification activity were assessed. Soil samples were collected between November 1995 and November 1996 from experimental plots established in 1971 that were annually treated for ten consecutive years with nine nitrogen (N) fertilizers and planted to corn. Each fertilizer was a ...
... Azospirillum brasilense is a nitrogen-fixing, root-colonizing bacterium that brings about plant-growth-promoting effects mainly because of its ability to produce phytohormones. Ethylenediamine (EDA)-resistant mutants of A. brasilense were isolated and screened for their higher ability to decrease acetylene and release ammonia in the medium. One of the mutants showed considerably higher levels of a ...
... Orientia tsutsugamushi, a causative agent of scrub typhus, is an obligate intracellular bacterium that requires the exploitation of the endocytic pathway in the host cell. We observed the localization of O. tsutsugamushi with clathrin or adaptor protein 2 within 30 min after the infection of nonprofessional phagocytes. We have further confirmed that the infectivity of O. tsutsugamushi is significa ...
... The objective of this paper was to study the adaptation dynamics and biochemical response of Azospirillum lipoferum grown in a continuous culture at various environmental shifts. The kinetics of A. lipoferum Sp 59b grown at steady states in a microaerobic chemostatic environment deviated from a typical Monod kinetics in both low and high dilution rates (D) due to several metabolic shifts that occu ...
... Polyhydroxyalkanoates (PHAs), intracellular carbon and energy reserve compounds in many bacteria, have been used extensively in biodegradable plastics. PHA formation is influenced by nutrient limitations and growth conditions. To characterize the PHA accumulation in a new denitrifying phosphorus-removing bacterium Brachymonas sp. P12, batch experiments were conducted in which the electron acceptor ...
... To isolate micro-organisms capable of utilizing polyhexamethylene biguanide (PHMB) as a sole source of nitrogen, and to demonstrate biodegradation of the biocide. Two consortia of bacteria were successfully enriched at the expense of PHMB, using sand from PHMB-treated swimming pools as inoculum. Both consortia were shown to contain bacteria belonging to the genera Sphingomonas, Azospirillum and Me ...
... Because excessive using of pesticides poses a threat to the environment and to human health, development of low-cost and sensitive methods for analysis of pesticides in the environment is needed. Several bacteria can release halide ions from the molecules of halogenated hydrocarbons. This can be used in a device for analysis of halogenated hydrocarbons in the environment by quantification of the h ...
... Cotton was cationized by exhaustion method using 3-chloro-2-hydroxypropyl trimethyl ammonium chloride (CHPTAC) as a cation-generating agent. Adsorption of silver nanoparticles on normal and cationized cotton was studied by exhaustion method at temperatures of 80°C and 100°C. Two exhaustion baths were used, containing nanosilver colloidal solutions stabilized by two different stabilizers and variou ...
... Intra-cellular pathogen, Listeria monocytogenes, is capable of invasion and survival within mammalian cells. However, Acanthamoeba polyphaga trophozoites phagocytose and rapidly degrade Listeria cells. In order to provide more information on amoeba phagocytosis and killing mechanisms, this study used several inhibitor agents known to affect the phagocytosis and killing of bacteria by eukaryotes. A ...
... Dietary nucleotides play an important role in the development of the gastrointestinal tract and may affect intestinal bacteria in young monogastrics. Two in vitro studies have been performed to evaluate the effects of individually added nucleotides (AMP, CMP, GMP, IMP, and UMP) at different concentrations on growth response of selected bacteria strains that dominate the piglets' small intestine. I ...
Pseudoxanthomonas; colloids; cations; scanning electron microscopy; cellulose; molecular weight; retention agents; hemicellulose; polystyrenes; monitoring; wood extractives; papermaking; adsorption; wood; atomic force microscopy; quartz; pH; pulp and paper industry; ammonium chloride; bacteria
Abstract:
... Quartz crystal microbalance with dissipation monitoring (QCM-D), atomic force microscopy (AFM) and field emission scanning electron microscopy (FESEM) were used as the tools to study the adsorption of bacteria onto surfaces of silica and polystyrene coated with materials related to papermaking. Cationic polyelectrolytes used as fixatives and retention aids in paper industry were found to promote i ...
... Aims: To gain an understanding of the environmental factors that affect the growth of the bacterium Sporosarcina pasteurii, the metabolism of the bacterium and the calcium carbonate precipitation induced by this bacterium to optimally implement the biological treatment process, microbial induced calcium carbonate precipitation (MICP), in situ. Methods and Results: Soil column and batch tests were ...
... We compared the efficacy of a natural biocide with four chemical tetrakishydroxymethyl phosphonium sulfonate, benzyl trimethyl ammonium chloride, and formaldehyde, glutaraldehyde, to control microbial induced corrosion in oil pipelines. The efficacy of biocides were monitored against Desulfovibrio vulgaris and Desulfovibrio gigas in experimental pipes by measuring cell counts, H₂S production, Fe(I ...
... The effects of nutritional conditions on growth and protease production by the feather-degrading Chryseobacterium sp. kr6 were investigated. Higher growth was observed on feather-containing or tryptone (TR) medium when compared to casein (CA) or glucose-nitrogen (GN) base medium. Protease production occurred during growth on feather-containing and TR media, whereas no protease activity was detecte ...
... Jonesia denitrificans BN-13 was isolated from a sample collected from Algerian soil. This strain produced extracellular xylanases, the best xylanolytic activity is obtained in a medium made up of xylan (7mg/ml), extract of yeast (2.5mg/ml), NaCl (3mg/ml), NH₄Cl (6mg/ml), and of MgSO₄ (0.3mg/ml). In fermentor it also proved that the maximum of xylanolytic activity (10.81U/ml) was observed at the en ...
Bacillus (bacteria); EDTA (chelating agent); ammonium chloride; bacteria; biotransformation; carbohydrates; casein; cheese whey; energy conservation; feather meal; feeds; fertilizers; gelatin; metal ions; pH; protein hydrolysates; proteinases; wastes
Abstract:
... Extracellular keratinase production by the feather-degrading Amazonian isolate Bacillus sp. P45 was evaluated with various growth substrates. Higher enzyme production occurred with feather meal (FM) in comparison to casein, gelatin, and cheese whey, suggesting the specificity of this strain for the utilization of keratinous substrates. Supplementation of FM medium with carbohydrates reduced enzyme ...
... The demulsifying bacterium XH1 was identified as a Bacillus mojavensis by the 16S rDNA gene. The extracellular biodemulsifier produced by this species was purified by ethanol extraction and column chromatography through a sephadex and silicon gel column. Preliminary investigation using UV–vis and TLC indicated that the biodemulsifier had two components a protein and a lipopeptide. All major compon ...
... The development of the nematode Caenorhabditis elegans is a highly dynamic process. Although various studies have assessed global transcriptome changes, information on the dynamics of the proteome during ontogenesis is not available. We metabolically labeled C. elegans by using ¹⁵N ammonium chloride as a precursor in Escherichia coli feeding bacteria grown in minimal media as a new cost-effective ...