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- Author:
- Hong Lu; Haihua Zhang; Qi Wang; Hanying Yuan; Wei He; Zhian Zhao; Yuyang Li
- Source:
- Current microbiology 2001 v.42 no.3 pp. 211-216
- ISSN:
- 0343-8651
- Subject:
- Escherichia coli; angiogenesis; anion exchange; bioactive properties; cell proliferation; chromatography; experimental design; inclusion bodies; interferon-gamma; nucleotide sequences; plasminogen; sodium chloride; solubilization; urea
- Abstract:
- ... The DNA sequence coding for plasminogen kringle 5 (pK5), an inhibitor of angiogenesis, was fused with that coding for interferon gamma and over-produced in the form of inactive inclusion bodies in E. coli. The amount of fusion protein was about 40% of total protein produced. The fusion protein contained in the inclusion bodies was solubilized in 8 m urea and purified by anion-exchange chromatograp ...
- DOI:
- 10.1007/s002840010206
- https://doi.org/10.1007/s002840010206
- Author:
- Wang, Jinfeng; Zhou, Leilei; Cai, Xin; Zou, Minji; Wang, Yuanyuan; Fu, Wenliang; Wang, Jiaxi; Xu, Donggang
- Source:
- Molecular biology reports 2010 v.37 no.8 pp. 3909-3913
- ISSN:
- 0301-4851
- Subject:
- personal development; insects; Escherichia coli; Komagataella pastoris; complementary DNA; growth and development; humans; methanol; neoplasms; recombinant proteins; reverse transcriptase polymerase chain reaction; rats; Chinese hamsters; animal ovaries; angiogenesis; angiopoietin-2
- Abstract:
- ... Angiopoietin-2 (Ang2) is a member of the Ang family. Its potential in clinical use has been highlighted for its important roles in angiogenesis during the individual development and the growth of tumors. Ang2 is difficult to be expressed in E. coli for its unique structure. The expressions of Ang2 in insect cells (Sf9) and Chinese hamster ovary (CHO) cell line have been reported, however, the larg ...
- DOI:
- 10.1007/s11033-010-0047-9
- https://doi.org/10.1007/s11033-010-0047-9
- Author:
- Kaleas, Kimberly A.; Schmelzer, Charles H.; Pizarro, Shelly A.
- Source:
- Journal of chromatography 2010 v.1217 no.2 pp. 235-242
- ISSN:
- 0021-9673
- Subject:
- Escherichia coli; agarose; angiogenesis; binding capacity; buffers; case studies; chromatography; crosslinking; desorption; feedstocks; human growth; humans; hydrochloric acid; hydrogen; hydrophobicity; inclusion bodies; industrialization; manufacturing; pH; protein content; proteins; resins; solubilization; tissue repair; urea; vascular endothelial growth factors
- Abstract:
- ... Mixed-mode chromatography resins are gaining popularity as effective purification tools for challenging feedstocks. This study presents the development of an industrial application to selectively capture recombinant human vascular endothelial growth factor (rhVEGF) on Capto MMC from an alkaline feedstock. Capto MMC resin contains a ligand that has the potential to participate in ionic, hydrophobic ...
- DOI:
- 10.1016/j.chroma.2009.07.023
- https://doi.org/10.1016/j.chroma.2009.07.023
- Author:
- Gang Liang; Catherine Butterfield; Jiwang Liang; Amy Birsner; Judah Folkman; Yuen Shing
- Source:
- Biochemical and biophysical research communications 2011 v.409 no.3 pp. 562-566
- ISSN:
- 0006-291X
- Subject:
- Escherichia coli; amino acids; angiogenesis; anticarcinogenic activity; cattle; chickens; chorioallantoic membrane; complementary DNA; endothelial cells; genes; humans; mass spectrometry; melanoma; mice; molecular weight; neoplasm cells; pancreatic neoplasms; recombinant proteins; severe combined immunodeficiency; transferrin
- Abstract:
- ... An angiogenesis inhibitor named Beta-35 has been identified and purified from the conditioned medium of mouse pancreatic β cells tumor cells. Beta-35 has a molecular weight of 35kDa and inhibits DNA synthesis of bovine capillary endothelial cells at a half-maximal concentration of approximately 5nM. It shows anti-angiogenic activity in the chick embryo chorioallantoic membrane at a dose of about 1 ...
- DOI:
- 10.1016/j.bbrc.2011.05.045
- https://doi.org/10.1016/j.bbrc.2011.05.045
- Author:
- Roman Esipov; Ksenia Beyrakhova; Vera Likhvantseva; Evgenia Stepanova; Vasily Stepanenko; Maria Kostromina; Yulia Abramchik; Anatoly Miroshnikov
- Source:
- Biochimie 2012 v.94 no.6 pp. 1368-1375
- ISSN:
- 0300-9084
- Subject:
- Escherichia coli; Tobacco etch virus; angiogenesis; anion exchange; blood vessels; collagen; cornea; dose response; endothelial cells; fibroblasts; ion exchange chromatography; mice; models; proteinases; rabbits; reversed-phase high performance liquid chromatography; viruses
- Abstract:
- ... Tumstatin, a cleavage fragment of collagen IV, is a potent endogenous inhibitor of angiogenesis. Tumstatin-derived peptide T8 possesses all angiostatic properties of full-length tumstatin and indirectly suppresses tumor growth. The potential of T8 to block pathological angiogenesis in the eye has not been explored yet. Here we assess antiangiogenic effects of a recombinant T8 peptide in rabbit cor ...
- DOI:
- 10.1016/j.biochi.2012.03.007
- https://doi.org/10.1016/j.biochi.2012.03.007
- Author:
- Wei-Cheng Chen; Shorong-Shii Liou; Thing-Fong Tzeng; Shiow-Ling Lee; I-Min Liu
- Source:
- BMC complementary and alternative medicine 2012 v.12 no.1 pp. 226
- ISSN:
- 1472-6882
- Subject:
- Candida albicans; Candida tropicalis; Escherichia coli; Lonicera japonica; Staphylococcus aureus; Staphylococcus epidermidis; aerial parts; alternative medicine; angiogenesis; anti-inflammatory activity; antimicrobial properties; cosmetics; cytokines; drugs; ethanol; excision; fibroblasts; flowering; healthy diet; hexosamines; histopathology; hydroxyproline; infectious diseases; medicinal plants; microorganisms; models; ointments; rats; reference standards; tissue repair
- Abstract:
- ... BACKGROUND: Lonicera japonica Thunb. (Caprifoliaceae), a widely used traditional Chinese medicinal plant, is used to treat some infectious diseases and it may have uses as a healthy food and applications in cosmetics and as an ornamental groundcover. The ethanol extract of the flowering aerial parts of L. japonica (LJEE) was investigated for its healing efficiency in a rat excision wound model. ME ...
- DOI:
- 10.1186/1472-6882-12-226
- PubMed:
- 23173654
- PubMed Central:
- PMC3577469
- https://doi.org/10.1186/1472-6882-12-226
- Author:
- Zhiyu Dai; Yifei Chen; Weiwei Qi; Lijun Huang; Yang Zhang; Ti Zhou; Xia Yang; Guoquan Gao
- Source:
- Preparative biochemistry & biotechnology 2013 v.43 no.1 pp. 123-136
- ISSN:
- 1532-2297
- Subject:
- humans; staining; resins; polyacrylamide gel electrophoresis; Western blotting; codons; amino acids; sodium dodecyl sulfate; liquid chromatography; antioxidant activity; algorithms; Escherichia coli
- Abstract:
- ... A unique serpin, kallistatin, displays vasodilatory, antiangiogenic, anti-inflammatory, and antioxidant activity. Difficulty and low efficacy of obtaining recombinant kallistatin limit the wide investigation of its biological and pathological function. The present study employed a codon optimization algorithm to redesign the kallistatin gene and achieved a high yield of recombinant kallistatin pro ...
- DOI:
- 10.1080/10826068.2012.712079
- https://doi.org/10.1080/10826068.2012.712079
- Author:
- B. V. Sunil Kumar; K. Aswani Kumar; K. Padmanath; Bhaskar Sharma; Meena Kataria
- Source:
- Animal biotechnology 2013 v.24 no.1 pp. 31-43
- ISSN:
- 1532-2378
- Subject:
- Canis lupus; EDTA (chelating agent); Escherichia coli; acetates; angiogenesis; apoptosis; bacterial proteins; breast neoplasms; dogs; extracellular matrix; gelatinase A; genes; genetic vectors; interstitial collagenase; kidneys; mammary neoplasms (animal); metastasis; trypsin; zinc
- Abstract:
- ... Matrix metalloproteinases (MMPs) are reported to be involved in tumor growth, apoptosis, angiogenesis, invasion, and development of metastases. These are zinc containing metalloproteases, known for their role in extracellular matrix degradation. MMP-11 (stromelysin3) is reported to be highly expressed in breast cancer, therefore it may act as marker enzyme for breast cancer progression. The presen ...
- DOI:
- 10.1080/10495398.2012.739978
- PubMed:
- 23394368
- https://doi.org/10.1080/10495398.2012.739978
- Author:
- Chan Li; Cui-Luan Yao
- Source:
- Fish & shellfish immunology 2013 v.34 no.3 pp. 799-809
- ISSN:
- 1050-4648
- Subject:
- nucleotides; fish; genetic markers; tissue repair; open reading frames; amino acids; spleen; Vibrio parahaemolyticus; signal peptide; superoxide anion; introns; tissues; interleukin-8; Escherichia coli; complementary DNA; Larimichthys crocea; immune response; kidneys; exons; single nucleotide polymorphism; quantitative polymerase chain reaction; liver; inflammation; neutrophils; angiogenesis
- Abstract:
- ... IL-8 plays a crucial role in acute inflammation by recruiting and mediating neutrophils and other cells and in initiating the oxidative burst in neutrophils and inducing wound healing by promoting angiogenesis. In the present study, the full-length cDNA and genome sequence of interleukin-8 (LcIL-8) were cloned from large yellow croaker Larimichthys crocea. The LcIL-8 cDNA sequence was 931 bp, cont ...
- DOI:
- 10.1016/j.fsi.2012.12.019
- https://doi.org/10.1016/j.fsi.2012.12.019
- Author:
- Gabriela Vazquez Rodriguez; Carmen Gonzalez; Antonio De Leon Rodriguez
- Source:
- Molecular biotechnology 2013 v.54 no.3 pp. 920-929
- ISSN:
- 1073-6085
- Subject:
- Escherichia coli; angiogenesis; bioactive properties; cell proliferation; chromatography; endothelial cells; genes; growth retardation; in vitro studies; metastasis; molecular weight; peptides; rats; therapeutics
- Abstract:
- ... Angiogenesis has been considered an important target for cancer therapy. The inhibition of angiogenesis represents a promising strategy for anti-cancer treatment, tumor growth inhibition, and metastasis. Vasostatin 30 (Vs30), and the 14.1 kDa vasoinhibin (Vi-II-14.1) are two peptides with remarkable anti-tumor and anti-angiogenic effect. The aim of this study was to produce a novel fusion protein ...
- DOI:
- 10.1007/s12033-012-9642-4
- PubMed:
- 23315240
- https://doi.org/10.1007/s12033-012-9642-4
- Author:
- Sun Kwon Bang; Young Sik Kim; Byung Soo Chang; Cheol Beom Park; In Seok Bang
- Source:
- Biotechnology and bioprocess engineering 2013 v.18 no.5 pp. 835-842
- ISSN:
- 1226-8372
- Subject:
- Escherichia coli; affinity chromatography; angiogenesis; chickens; chorioallantoic membrane; endothelial cells; genes; humans; inclusion bodies; plasmids; proteins; vascular endothelial growth factors
- Abstract:
- ... Vascular endothelial growth factors (VEGFs) are a family of proteins that promote angiogenesis and participate in a variety of physiological and pathological processes. In this work, the gene encoding the human VEGF isoform 165 (hVEGF₁₆₅) was cloned into the expression vector pET32a (+) to construct a fusion expression plasmid that induced the thioredoxin (Trx) gene and transformed into Escherichi ...
- DOI:
- 10.1007/s12257-012-0829-0
- https://doi.org/10.1007/s12257-012-0829-0
- Author:
- Fengmei Li; Li Xu; Xuemei Gai; Zhi Zhou; Lingling Wang; Huan Zhang; Yunchao Gai; Linsheng Song; Jiansheng Yu; Chengwei Liang
- Source:
- Fish & shellfish immunology 2013 v.35 no.4 pp. 1240-1248
- ISSN:
- 1050-4648
- Subject:
- dopamine; cell movement; crabs; Komagataella pastoris; genes; cell differentiation; heart; Eriocheir sinensis; open reading frames; fluorescence; recombinant proteins; Vibrio anguillarum; gene expression; hepatopancreas; signal peptide; vascular endothelial growth factors; neurodevelopment; cysteine; tissues; Escherichia coli; fluorometry; platelet-derived growth factor; messenger RNA; complementary DNA; muscles; immune response; cell proliferation; norepinephrine; quantitative polymerase chain reaction; neurosecretory system; angiogenesis; hemocytes
- Abstract:
- ... Members of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family have been implicated in cell proliferation, cell differentiation, and cell migration, vascular development, angiogenesis and neural development. In the present study, a novel PDGF/VEGF related factor gene was cloned and identified in Chinese mitten crab Eriocheir sinensis (designated as EsPVF1). The ...
- DOI:
- 10.1016/j.fsi.2013.07.042
- https://doi.org/10.1016/j.fsi.2013.07.042
- Author:
- Jinhua Li; Guifang Wang; Donghui Wang; Qianju Wu; Xinquan Jiang; Xuanyong Liu
- Source:
- Journal of colloid and interface science 2014 v.436 pp. 160-170
- ISSN:
- 0021-9797
- Subject:
- Escherichia coli; Staphylococcus aureus; alkali treatment; alkalosis; angiogenesis; antibacterial properties; bacteria; bacterial adhesion; bone formation; bone marrow; cell proliferation; human cell lines; humans; hydrolysis; models; nanopores; neoplasm cells; neoplasms; pH; salts; stem cells; titanium
- Abstract:
- ... Many attentions have been paid to the beneficial effect of alkali-treated titanium to bioactivity and osteogenic activity, but few to the other biological effect. In this work, hierarchical micro/nanopore films were prepared on titanium surface by acid etching and alkali treatment and their biological effects on bacteria, cancer cells and mesenchymal stem cells were investigated. Gram-positive Sta ...
- DOI:
- 10.1016/j.jcis.2014.08.053
- https://doi.org/10.1016/j.jcis.2014.08.053
- Author:
- Qianju Wu; Jinhua Li; Wenjie Zhang; Haixin Qian; Wenjun She; Hongya Pan; Jin Wen; Xiuli Zhang; Xuanyong Liu; Xinquan Jiang
- Source:
- Journal of materials chemistry B 2014 v.2 no.39 pp. 6738-6748
- ISSN:
- 2050-7518
- Subject:
- Escherichia coli; alkaline phosphatase; angiogenesis; antibacterial properties; bone formation; bone marrow; chemical composition; coatings; copper; cytotoxicity; enzyme activity; genes; hot water treatment; hypoxia-inducible factor 1; oxidation; rats; stem cells; titanium dioxide; topography; vascular endothelial growth factors
- Abstract:
- ... Numerous efforts have been made to modify the surface topography and chemical composition of biomedical implants in order to enhance the antibacterial ability and the osteointegration between implants and surrounding bone tissue. In the present work, copper-incorporated TiO₂ coatings were fabricated by combining micro-arc oxidation and hydrothermal treatment together to functionalize the surface o ...
- DOI:
- 10.1039/c4tb00923a
- https://doi.org/10.1039/c4tb00923a
- Author:
- Sophie Winkler; Daniela Schweiger; Zheng Wei; Erich Rajkovic; Andreas J. Kungl
- Source:
- Carbohydrate research 2014 v.389 pp. 72-77
- ISSN:
- 0008-6215
- Subject:
- Escherichia coli; angiogenesis; circular dichroism spectroscopy; enzyme inhibitors; extracellular matrix; heparan sulfate; humans; metastasis; screening
- Abstract:
- ... Human heparanase is a heparan sulfate degrading enzyme located in the extracellular matrix playing a decisive role in angiogenesis and tumor metastasis. Translated as a 65kDa inactive prae-form, the protein is processed into an 8kDa and a 50kDa subunit which form a non-covalently associated active heterodimer. We have expressed the two subunits separately in Escherichia coli which yielded active h ...
- DOI:
- 10.1016/j.carres.2014.01.002
- https://doi.org/10.1016/j.carres.2014.01.002
- Author:
- Y. Ma; X.-B. Jin; F.-J. Chu; D.-Mei Bao; J.-Y. Zhu
- Source:
- Applied microbiology and biotechnology 2014 v.98 no.18 pp. 7923-7933
- ISSN:
- 0175-7598
- Subject:
- Escherichia coli; affinity chromatography; angiogenesis; chicks; chorioallantoic membrane; culture media; drugs; gene fusion; hepatoma; human umbilical vein endothelial cells; humans; liver
- Abstract:
- ... Recombinant human endostatin (rEndostatin or endostar) has been shown to inhibit endothelial cells proliferation, migration, and angiogenesis and exhibits a broad spectrum of activities against solid tumors. However, rEndostatin is easily degradable and evenly distributed to all tissues. Selectively delivering rEndostatin to the lesion site might be more potent. The circumsporozoite protein (CSP) ...
- DOI:
- 10.1007/s00253-014-5818-0
- PubMed:
- 24908076
- https://doi.org/10.1007/s00253-014-5818-0
- Author:
- Kiran Kumar Chereddy; Charles-Henry Her; Michela Comune; Claudia Moia; Alessandra Lopes; Paolo E. Porporato; Julie Vanacker; Martin C. Lam; Lars Steinstraesser; Pierre Sonveaux; Huijun Zhu; Lino S. Ferreira; Gaëlle Vandermeulen; Véronique Préat
- Source:
- Journal of controlled release 2014 v.194 pp. 138-147
- ISSN:
- 0168-3659
- Subject:
- Escherichia coli; angiogenesis; antimicrobial properties; biodegradability; cell movement; collagen; drug delivery systems; granulation tissue; health services; humans; interleukin-6; keratinocytes; lactic acid; metabolism; nanoparticles; tissue repair; wound treatment
- Abstract:
- ... Wound treatment remains one of the most prevalent and economically burdensome healthcare issues in the world. Poly (lactic-co-glycolic acid) (PLGA) supplies lactate that accelerates neovascularization and promotes wound healing. LL37 is an endogenous human host defense peptide that modulates wound healing and angiogenesis and fights infection. Hence, we hypothesized that the administration of LL37 ...
- DOI:
- 10.1016/j.jconrel.2014.08.016
- https://doi.org/10.1016/j.jconrel.2014.08.016
- Author:
- Roghaye Arezumand; Reza Mahdian; Mahdi Behdani; Hossein Khanahmad; Jahangir Langari; Nabiollah Namvarasl; Reza Hassanzadeh-Ghasabeh; Sirous Zeinali
- Source:
- Saudi Journal of Biological Sciences 2014 v.21 pp. 35-39
- ISSN:
- 1319-562X
- Subject:
- Camelidae; Escherichia coli; Western blotting; angiogenesis; antigens; antiserum; blood serum; camels; enzyme-linked immunosorbent assay; humans; inflammation; ischemia; plasmids; polyclonal antibodies; recombinant proteins; vascular endothelial growth factors
- Abstract:
- ... Placental growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family. Unlike VEGF, PlGF is dispensable for normal cell development as well as playing various roles in pathological angiogenesis which occurs in tissue ischemia, inflammation, and malignancy. The PlGF-1 has been considered as a potential candidate for the diagnosis and targeting of pathological angiogenes ...
- DOI:
- 10.1016/j.sjbs.2013.04.008
- PubMed:
- 24596498
- PubMed Central:
- PMC3937466
- https://doi.org/10.1016/j.sjbs.2013.04.008
- Author:
- Evelien Wynendaele; Frederick Verbeke; Matthias D’Hondt; An Hendrix; Christophe Van De Wiele; Christian Burvenich; Kathelijne Peremans; Olivier De Wever; Marc Bracke; Bart De Spiegeleer
- Source:
- Peptides 2015 v.64 pp. 40-48
- ISSN:
- 0196-9781
- Subject:
- Bacillus (bacteria); Enterococcus faecium; Escherichia coli; angiogenesis; bacteria; colorectal neoplasms; humans; hydrogen sulfide; intestinal microorganisms; metastasis; microbiome; mutagens; neoplasm cells; nitrogen; oxygen; peptides; quorum sensing; virulence
- Abstract:
- ... To date, the precise role of the human microbiome in health and disease states remains largely undefined. Complex and selective crosstalk systems between the microbiome and mammalian cells are also not yet reported. Research up till now mainly focused on bacterial synthesis of virulence factors, reactive oxygen/nitrogen species (ROS/RNS) and hydrogen sulphide, as well as on the activation of exoge ...
- DOI:
- 10.1016/j.peptides.2014.12.009
- https://doi.org/10.1016/j.peptides.2014.12.009
20. Tat PTD–endostatin: A novel anti-angiogenesis protein with ocular barrier permeability via eye-drops
- Author:
- Xinke Zhang; Yan Li; Yanna Cheng; Haining Tan; Zhiwei Li; Yi Qu; Guoying Mu; Fengshan Wang
- Source:
- BBA - General Subjects 2015 v.1850 pp. 1140-1149
- ISSN:
- 0304-4165
- Subject:
- Escherichia coli; angiogenesis; cell proliferation; chickens; chicks; chorioallantoic membrane; chromatography; denaturation; drugs; endothelial cells; flow cytometry; fluorescence; genetic engineering; immunohistochemistry; inclusion bodies; intraocular injection; mice; models; permeability; pharmacology; retina
- Abstract:
- ... Endostatin, a specific inhibitor of endothelial cell proliferation and angiogenesis, has been proved to have effects on ocular neovascular diseases by intraocular injection. In order to increase its permeability to ocular barriers and make it effective on fundus oculi angiogenesis diseases via non-invasive administration (eye drops), endostatin was fused to Tat PTD via a genetic engineering method ...
- DOI:
- 10.1016/j.bbagen.2015.01.019
- https://doi.org/10.1016/j.bbagen.2015.01.019
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