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1. Efficient secretion of human endostatin in the yeast, Pichia pastoris

Author:
Jubao Duan; Xin Cai; Baolin Zhang; Yuzhen Li; Minji Zou; Jiaxi Wang
Source:
Biotechnology letters 1999 v.21 no.12 pp. 1095-1099
ISSN:
0141-5492
Subject:
liver; complementary DNA; Komagataella pastoris; humans; secretion; brain; yeasts; collagen; rats; angiogenesis
Abstract:
... Endostatin is a 20 kDa COOH-terminal fragment of collagen XVIII that inhibits angiogenesis and tumor growth. The cDNA coding for human endostatin in human fetal liver has been cloned into the secreting expression organism Pichia pastoris, and the high level expression of human endostatin has been achieved (about 200 mg of endostatin in 1 l of culture). The recombinant human endostatin was purified ...
DOI:
10.1023/A:1005670009198

2. p53-Mediated Inhibition of Angiogenesis Through Up-Regulation of a Collagen Prolyl Hydroxylase

Author:
Teodoro, Jose G.; Parker, Albert E.; Zhu, Xiaochun; Green, Michael R.
Source:
Science 2006 v.313 no.5789 pp. 968-971
ISSN:
0036-8075
Subject:
angiogenesis; collagen; endothelial cells; genes; growth retardation; humans; mice; neoplasms; procollagen-proline dioxygenase; therapeutics; transcription (genetics)
Abstract:
... Recent evidence suggests that antiangiogenic therapy is sensitive to p53 status in tumors, implicating a role for p53 in the regulation of angiogenesis. Here we show that p53 transcriptionally activates the α(II) collagen prolyl-4-hydroxylase [α(II)PH] gene, resulting in the extracellular release of antiangiogenic fragments of collagen type 4 and 18. Conditioned media from cells ectopically expres ...
DOI:
10.1126/science.1126391

3. Effects of Ca2+ channel blocker verapamil on tissue regeneration in a lizard tail autotomy model: a biochemical and histological study

Author:
Mehmet Turgut; Yücel B. Koca; Süleyman Kaplan; Kubilay Metin; Nazan Üzüm; Emrah Soylu; Aziz Avcı; Murat Rağbetli; Elif P. Beytaş; Kurtuluş Olgun
Source:
Amphibia-reptilia v.28 no.1 pp. 7-15
ISSN:
0173-5373
Subject:
adults; angiogenesis; antagonists; autotomy; calcium; cell proliferation; collagen; fibroblasts; histology; lizards; models; neurogenesis; tail; tissue repair; verapamil
Abstract:
... Ca2+ ions have been reported to augment the activities of many cell types including cellular proliferation and tissue regeneration. Moreover, it is well known that verapamil is a L-type voltage-gated Ca2+ antagonist with important clinical implications. To evaluate the role of Ca2+ ions in the regeneration of tail in lizards, verapamil was used in vivo to modulate the activity of intracellular Ca2 ...
DOI:
10.1163/156853807779798992

4. High level expression of human endostatin in Pichia pastoris using a synthetic gene construct

Author:
Zhijian Su; Xiaoping Wu; Ya Feng; Changcai Ding; Yechen Xiao; Lu Cai; Wenke Feng; Xiaokun Li
Source:
Applied microbiology and biotechnology 2007 v.73 no.6 pp. 1355-1362
ISSN:
0175-7598
Subject:
liquid state fermentation; endothelial cells; Komagataella pastoris; humans; recombinant proteins; bioreactors; yeasts; collagen; codons; heparin; synthetic genes; affinity chromatography; fibroblasts; angiogenesis
Abstract:
... Endostatin, a 20-kDa C-terminal fragment derived from type XVIII collagen, is a potent angiogenesis inhibitor and an antitumor factor. To improve the production of recombinant human endostatin on increasing demand in clinical practice, we constructed an artificial gene encoding its mature peptide sequence in human collagen XVIII. The synthetic gene consisted of 20 codons in preference in methylotr ...
DOI:
10.1007/s00253-006-0604-2
PubMed:
17115211

5. Human neutrophils uniquely release TIMP-free MMP-9 to provide a potent catalytic stimulator of angiogenesis

Author:
Ardi, Veronica C.; Kupriyanova, Tatyana A.; Deryugina, Elena I.; Quigley, James P.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2007 v.104 no.51 pp. 20262-20267
ISSN:
0027-8424
Subject:
angiogenesis; catalytic activity; chickens; chorioallantoic membrane; collagen; fibrosarcoma; gelatinase B; humans; models; neoplasm cells; neutrophils; secretory granules
Abstract:
... Several lines of evidence have implicated matrix metalloproteinase 9 (MMP-9) as a protease inducing an angiogenic switch critical for tumor progression. Among MMP-9-expressing cell types, including cancer cells and tumor-associated leukocytes, inflammatory neutrophils appear to provide an important source of MMP-9 for tumor angiogenesis. However, delivery of MMP-9 by neutrophils has not been mecha ...
DOI:
10.1073/pnas.0706438104
PubMed:
18077379
PubMed Central:
PMC2154419

6. Ovarian wedge resection restores fertility in estrogen receptor β knockout (ERβ⁻/⁻) mice

Author:
Inzunza, José; Morani, Andrea; Cheng, Guojun; Warner, Margaret; Hreinsson, Julius; Gustafsson, Jan-Åke; Hovatta, Outi
Source:
Proceedings of the National Academy of Sciences of the United States of America 2007 v.104 no.2 pp. 600-605
ISSN:
0027-8424
Subject:
actin; angiogenesis; anovulation; blood; collagen; estrogen receptors; follicle-stimulating hormone receptors; follicular development; genes; granulosa cells; knockout mutants; males; mice; platelet-derived growth factor receptors; polycystic ovary syndrome; resection; smooth muscle; women
Abstract:
... Ovulation rarely occurs in mice in which the estrogen receptor β (ERβ) gene has been inactivated (ERβ⁻/⁻ mice). Here, we investigated whether this subfertility is due to a defect in the ovary itself or to more general endocrine changes in ERβ⁻/⁻ mice. We transplanted ERβ⁻/⁻ ovaries into WT mice and WT ovaries into ERβ⁻/⁻ mice. Upon mating with ERβ⁻/⁻ males, fertility increased from 20% in control ...
DOI:
10.1073/pnas.0608951103
PubMed:
17197418
PubMed Central:
PMC1766431

7. Dynamic changes in messenger RNA profiles of bovine endometrium during the oestrous cycle: Focus on Mammalian Embryogenomics

Author:
Mitko, Katrin; Ulbrich, Susanne E.; Wenigerkind, Hendrik; Sinowatz, Fred; Blum, Helmut; Wolf, Eckhard; Bauersachs, Stefan
Source:
Reproduction 2008 v.135 no.2 pp. 225-240
ISSN:
1470-1626
Subject:
Simmental; angiogenesis; biochemical pathways; cell adhesion; cell growth; collagen; complementary DNA; diestrus; endometrium; estrogens; estrus; extracellular matrix; gene expression; gene interaction; genes; heifers; immune response; messenger RNA; metestrus; microarray technology; morphogenesis; oxytocin; progesterone; tight junctions; transcriptome
Abstract:
... During the oestrous cycle, the bovine endometrium exhibits characteristic morphological and functional changes, which are mainly induced by progesterone (P₄), oestrogens and oxytocin. We studied the response of the endometrium to this changing hormonal environment at the transcriptome level using a custom-made cDNA microarray. Endometrium samples were recovered from Simmental heifers on days 0 (oe ...
DOI:
10.1530/REP-07-0415

8. Effect of SBD.4A – a defined multicomponent preparation of Angelica sinensis – in periodontal regeneration models

Author:
Hui Zhao; Alexei Alexeev; Vrushali Sharma; Lorenzo Dave T. Guzman; Krzysztof Bojanowski
Source:
Phytotherapy research 2008 v.22 no.7 pp. 923-928
ISSN:
0951-418X
Subject:
Angelica sinensis; acid deposition; angiogenesis; bones; collagen; disease course; growth and development; growth factors; hyaluronic acid; ligaments; medicinal plants; models; osteoblasts; rats; surgery; teeth
Abstract:
... Periodontitis is a major cause of tooth motility and loss, resulting in destruction of the supporting structures of the tooth, including periodontal ligaments and alveolar bone. Periodontal surgery can slow the progression of the disease, but is costly, invasive, limited by contraindications and technique‐sensitive. Recently, non‐invasive pharmacological treatments using proteinaceous biologicals ...
DOI:
10.1002/ptr.2421
PubMed:
18389473

9. FT-IR spectral imaging of blood vessels reveals protein secondary structure deviations induced by tumor growth

Author:
Katia Wehbe; Raphael Pinneau; Michel Moenner; Gérard Déléris; Cyril Petibois
Source:
Analytical and bioanalytical chemistry 2008 v.392 no.1-2 pp. 129-135
ISSN:
1618-2642
Subject:
Fourier transform infrared spectroscopy; angiogenesis; basement membrane; blood; blood capillaries; collagen; genetic markers; image analysis; membrane proteins; mice; neoplasms; protein secondary structure
Abstract:
... Vascular basement membrane remodeling is involved in tumor angiogenesis to enable tumor invasion and growth. FT-IR spectral imaging was used to determine changes in tumor blood vessels to reveal protein secondary structure in Rag-gamma immuno-deficient mice sacrificed 14 and 21 days after subcutaneous glioma implantation. For the oldest blood capillaries (diameter >20 microns), tumor growth induce ...
DOI:
10.1007/s00216-008-2230-6
PubMed:
18636246

10. Madecassoside isolated from Centella asiatica herbs facilitates burn wound healing in mice

Author:
Liu, M.; Dai, Y.; Li, Y.; Luo, Y.; Huang, F.; Gong, Z.; Meng, Q.
Source:
Planta medica 2008 v.74 no.8 pp. 809-815
ISSN:
0032-0943
Subject:
Centella asiatica; medicinal plants; Oriental traditional medicine; triterpenoids; phytochemicals; burns (injuries); tissue repair; antioxidant activity; animal disease models; epithelial cells; fibroblasts; cell differentiation; collagen; angiogenesis
DOI:
10.1055/s-2008-1074533
PubMed:
18484522

11. Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: standardization of semi-quantitative and quantitative histological assessments

Author:
P. Gal; M. Mokry; B. Vidinsky; T. Vasilenko; S. Mozes; N. Bobrov; Z. Tomori; J. Bober; L. Lenhardt
Source:
Veterinární medicína v.53 no.12 pp. 652-659
ISSN:
0375-8427
Subject:
adrenal cortex hormones; angiogenesis; collagen; fibroblasts; granulation tissue; histology; leukocytes; macrophages; males; quantitative analysis; surgery
Abstract:
... The use of a simple and reproducible model is inevitable for objective statement of the effects of external factors on wound healing. Hence, present study was conducted to establish an excisional model of skin wound healing in corticosteroid treated, and streptozotocine induced diabetic rats as well as to standardized the semi-quantitative and quantitative evaluation of selected parameters. Round ...
DOI:
10.17221/1973-vetmed

12. Spatially defined oxygen gradients and vascular endothelial growth factor expression in an engineered 3D cell model

Author:
Cheema, U.; Brown, R. A.; Alp, B.; MacRobert, A. J.
Source:
Cellular and molecular life sciences 2008 v.65 no.1 pp. 177-186
ISSN:
1420-682X
Subject:
angiogenesis; angiogenic proteins; collagen; hypoxia; models; monitoring; nutrients; oxygen; oxygen consumption; tissues; vascular endothelial growth factors; viability
Abstract:
... Tissue hypoxia results in rapid angiogenesis in vivo, triggered by angiogenic proteins, including vascular endothelial growth factor (VEGF). Current views of tissue viability are founded on whether 'deeper-lying' cells receive sufficient nutrients and oxygen for normal activity and ultimately survival. For intact tissues, levels of such essential nutrients are governed by micro-vascular perfusion. ...
DOI:
10.1007/s00018-007-7356-8
PubMed:
17994289

13. Cardiac cells implanted into a cylindrical, vascularized chamber in vivo: pressure generation and morphology

Author:
Birla, Ravi K.; Dhawan, Vikas; Dow, Douglas E.; Huang, Yen-Chih; Brown, David L.
Source:
Biotechnology letters 2009 v.31 no.2 pp. 191-201
ISSN:
0141-5492
Subject:
angiogenesis; animals; collagen; elderly; electrical treatment; electron microscopy; gap junctions; gels; myocardium; silicone
Abstract:
... We have previously described a model to implant dissociated cells into a cylindrical, vascularized bed in vivo to promote the formation of functional cardiac muscle constructs. We now investigate the cellular organization and the ability of the constructs to generate intra-luminal pressure. Primary cardiac cells were isolated from hearts of 2-3 day old rats, suspended in fibrin gel and inserted in ...
DOI:
10.1007/s10529-008-9859-2
PubMed:
18854950

14. Multifunctionality of extracellular and cell surface heparan sulfate proteoglycans

Author:
Kirn-Safran, Catherine; Farach-Carson, Mary C.; Carson, Daniel D.
Source:
Cellular and molecular life sciences 2009 v.66 no.21 pp. 3421-3434
ISSN:
1420-682X
Subject:
N-acetylglucosamine; angiogenesis; biofiltration; collagen; enzymes; glucuronic acid; heparan sulfate; morphogenesis; polymers; proteoglycans; tissue repair; tissues
Abstract:
... Heparan sulfate proteoglycans are a remarkably diverse family of glycosaminoglycan-bearing protein cores that include the syndecans, the glypicans, perlecan, agrin, and collagen XVIII. Members of this protein class play key roles during normal processes that occur during development, tissue morphogenesis, and wound healing. As key components of basement membranes in organs and tissues, they also p ...
DOI:
10.1007/s00018-009-0096-1

15. Adenovirus-mediated stromal cell-derived factor-1 alpha gene transfer improves cardiac structure and function after experimental myocardial infarction through angiogenic and antifibrotic actions

Author:
Tang, Junming; Wang, Jianing; Song, Hongxian; Huang, Yongzhang; Yang, Jianye; Kong, Xia; Guo, Linyun; Zheng, Fei; Zhang, Lei
Source:
Molecular biology reports 2010 v.37 no.4 pp. 1957-1969
ISSN:
0301-4851
Subject:
angiogenesis; chemoattractants; collagen; coronary vessels; gene expression; gene transfer; infarction; messenger RNA; models; myocardial infarction; myocardium; rats; stem cells; transforming growth factor beta 1
Abstract:
... Stromal cell-derived factor 1α (SDF-1) is not only a major chemotactic factor, but also an inducer of angiogenesis. The effects of SDF-1α on the left ventricular remodeling in a rat myocardial infarction (MI) model were analyzed. Myocardial infarction was induced by ligation of the left coronary artery in rats. 0.5 × 10¹⁰ pfu/ml AdV-SDF-1 or 0.5 × 10¹⁰ pfu/ml Adv-LacZ were immediately injected int ...
DOI:
10.1007/s11033-009-9642-z
PubMed:
19653123
PubMed Central:
PMC2831180

16. Canine pre-iridal fibrovascular membranes: morphologic and immunohistochemical investigations

Author:
Zarfoss, Mitzi K.; Breaux, Carrie B.; Whiteley, Herbert E.; Hamor, Ralph E.; Flaws, Jodi A.; Labelle, Philippe; Dubielzig, Richard R.
Source:
Veterinary ophthalmology 2010 v.13 no.1 pp. 4-13
ISSN:
1463-5216
Subject:
actin; adenoma; angiogenesis; cell adhesion; collagen; dog diseases; dogs; endothelial cells; extracellular matrix; eyes; immunohistochemistry; laminin; mucins; smooth muscle; vascular endothelial growth factors
Abstract:
... Pathologic intraocular neovascularization is a key component of many canine ophthalmic diseases such as uveitis, retinal detachment, intraocular neoplasms, and corneal perforation. The purpose of this study was to evaluate the structure of pre-iridal fibrovascular membranes (PIFMs) associated with several different disease processes and to identify specific factors associated with their developmen ...
DOI:
10.1111/j.1463-5224.2009.00739.x
PubMed:
20149169

17. The Effect of AlloDerm on the Initiation and Growth of Human Neovessels

Author:
Weiss, Sean R.; Tenney, Justin M.; Thomson, Jessica L.; Anthony, Catherine T.; Chiu, Ernest S.; Friedlander, Paul L.; Woltering, Eugene A.
Source:
Laryngoscope 2010 v.120 no.3 pp. 443-449
Subject:
collagen; surgical equipment; regrowth; blood veins; cell growth; endothelial cells; tissue repair; angiogenesis
Abstract:
... Objectives/Hypothesis: AlloDerm (LifeCell Corp., Branchburg, NJ) is commonly employed for reconstruction of ablative soft tissue and mucosal defects following surgical resections. Although devoid of growth factors, AlloDerm may serve as an adhesive matrix for binding of growth factors, increasing local angiogenesis, and wound healing. We hypothesized that AlloDerm would enhance angiogenesis and mi ...
Handle:
10113/43651
DOI:
10.1002/lary.20679
PubMed:
20131371

18. Healing potential of Rosmarinus officinalis L. on full-thickness excision cutaneous wounds in alloxan-induced-diabetic BALB/c mice

Author:
Abu-Al-Basal, Mariam A.
Source:
Journal of ethnopharmacology 2010 v.131 no.2 pp. 443-450
ISSN:
0378-8741
Subject:
Rosmarinus officinalis; rosemary; medicinal plants; herbal medicines; tissue repair; animal injuries; mice; animal models; diabetes mellitus; skin lesions; intraperitoneal injection; plant extracts; essential oils; topical application; blood glucose; body weight; granulation tissue; histology; inflammation; angiogenesis; collagen
Abstract:
... Aim of the study: Rosmarinus officinalis (Rosemary) used in Jordanian folk medicine for wound management and treatment. Therefore, the present study was conducted to assess the healing efficacy of both aqueous extract and essential oil of the aerial parts on alloxan-induced diabetic BALB/c mice. Materials and methods: Two full-thickness round wounds were created in the dorsal area of each mouse. A ...
DOI:
10.1016/j.jep.2010.07.007
PubMed:
20633625

19. In vitro and ex vivo antiangiogenic activity of salvia officinalis

Author:
Keshavarz, Maryam; Mostafaie, Ali; Mansouri, Kamran; Bidmeshkipour, Ali; Motlagh, Hamid Reza Mohammadi; Parvaneh, Shahram
Source:
Phytotherapy research 2010 v.24 no.10 pp. 1526-1531
ISSN:
0951-418X
Subject:
Salvia officinalis; acetates; aerial parts; angiogenesis; aorta; cell proliferation; collagen; endothelial cells; ethanol; herbs; metastasis; models; rats; tissue repair; umbilical veins
Abstract:
... Angiogenesis is a key process in the promotion of cancer and its metastasis. Herein, the antiangiogenic activity of Salvia officinalis extract and its fractions was investigated. S. officinalis aerial parts were extracted with ethanol and its successive hexane, ethyl acetate, n-butanol and aqueous fractions were evaluated for their antiangiogenic activities using human umbilical vein endothelial c ...
DOI:
10.1002/ptr.3168
PubMed:
20878705

20. The expression of tumstatin is down-regulated in renal carcinoma

Author:
Xu, Chun-xiao; Liu, Xian-xi; Hou, Guo-sheng; Yan, Yun-fei; Chen, Shi-min; Wang, Wei; Jiang, Guang-shui; Liu, Bin; Xin, Jia-xuan
Source:
Molecular biology reports 2010 v.37 no.5 pp. 2273-2277
ISSN:
0301-4851
Subject:
Western blotting; angiogenesis; carcinoma; cell proliferation; collagen; endothelial cells; gene expression; gene expression regulation; genes; humans; messenger RNA; polyclonal antibodies; prognosis; reverse transcriptase polymerase chain reaction; therapeutics; tissues
Abstract:
... Tumstatin is the 28 kDa NC1 domain of the α3 chain of type IV collagen that inhibits pathological angiogenesis and suppresses endothelial cell proliferation and tumor growth. In the present paper, we expressed and purified recombinant human tumstatin protein and then prepared the anti-tumstatin polyclonal antibody. To investigate the expression of tumstatin in renal carcinoma, tumstatin protein wa ...
DOI:
10.1007/s11033-009-9718-9
PubMed:
19688274

21. Biodegradable collagen patch with covalently immobilized VEGF for myocardial repair

Author:
Yasuo Miyagi; Loraine L.Y. Chiu; Massimo Cimini; Richard D. Weisel; Milica Radisic; Ren-Ke Li
Source:
Biomaterials 2011 v.32 no.5 pp. 1280-1290
ISSN:
0142-9612
Subject:
angiogenesis; biodegradability; blood vessels; bone marrow cells; cell viability; collagen; correlation; rats; tissues; transfection; vascular endothelial growth factors
Abstract:
... Vascularization of engineered tissues in vitro and in vivo remains a key problem in translation of engineered tissues to clinical practice. Growth factor signalling can be prolonged by covalent tethering, thus we hypothesized that covalent immobilization of vascular endothelial growth factor (VEGF-165) to a porous collagen scaffold will enable rapid vascularization in vivo. Covalent immobilization ...
DOI:
10.1016/j.biomaterials.2010.10.007

22. Controlled release of thymosin β4 using collagen–chitosan composite hydrogels promotes epicardial cell migration and angiogenesis

Author:
Loraine L.Y. Chiu; Milica Radisic
Source:
Journal of controlled release 2011 v.155 no.3 pp. 376-385
ISSN:
0168-3659
Subject:
adults; angiogenesis; cell movement; chitosan; collagen; electrostatic interactions; encapsulation; explants; growth factors; hydrocolloids; mice; myocardial infarction; myocytes; peptides; rats; scanning electron microscopy; smooth muscle; subcutaneous injection; thymosin
Abstract:
... Rapid vascularization at the infarcted site is crucial for cardiac repair following myocardial infarction. Thymosin β4 (Tβ4), a 43-amino acid peptide, is both angiogenic and cardioprotective. Tβ4 in soluble form was previously shown to promote cell migration from quiescent adult cardiac explants. Here we developed a collagen–chitosan hydrogel for the encapsulation of Tβ4, which allowed its control ...
DOI:
10.1016/j.jconrel.2011.05.026

23. Developmental effects related to angiogenesis and osteogenic differentiation in Salmon larvae continuously exposed to dioxin-like 3,3′,4,4′-tetrachlorobiphenyl (congener 77)

Author:
Marianne Olufsen; Augustine Arukwe
Source:
Aquatic toxicology 2011 v.105 no.3-4 pp. 669-680
ISSN:
0166-445X
Subject:
RNA; anemia; angiogenesis; arrhythmia; bone formation; bone morphogenetic proteins; chondrogenesis; collagen; edema; eggs; genes; heart rate; larvae; quantitative polymerase chain reaction; receptors; salmon; transcription (genetics); transforming growth factors
Abstract:
... We have studied the effects of dioxin-like 3,3′,4,4′-tetrachlorobiphenyl (PCB-77) on developmental effects related to angiogenesis and osteogenesis during early life-stages of salmon. Larvae were kept at 6°C and continuously exposed to waterborne PCB-77 (1 or 10ng/L) initiated at the egg stage or 416-day degrees (dd) and throughout yolk-sac stage (716dd) and for a total duration of 50 days (or 300 ...
DOI:
10.1016/j.aquatox.2011.09.005
PubMed:
21979385

24. Evaluation of the effectiveness of an ointment based on Alkannins/Shikonins on second intention wound healing in the dog

Author:
Karayannopoulou, Maria; Tsioli, Tassiliki; Loukopoulos, Panayiotis; Anagnostou, Tilemahos L.; Giannakas, Nikolaos; Savvas, Ioannis; Papazoglou, Lysimachos G.; Kaldrymidou, Eleni
Source:
Canadian journal of veterinary research 2011 v.75 no.1 pp. 42-48
ISSN:
0830-9000
Subject:
angiogenesis; collagen; dogs; humans; naphthoquinones; ointments; tissue repair
Abstract:
... The enantiomeric naphthoquinones alkannins and shikonins (A/S) have been established as potent wound healing agents. The purpose of this study was to evaluate the effectiveness of an A/S based ointment for humans on second intention wound healing in the dog, as compared to wound flushing with Lactated Ringer’s solution (LRS). Ten mixed breed dogs, aged 2 to 5 y, were used. One 2.5 × 2.5 cm full-th ...

25. Growth factor/heparin-immobilized collagen gel system enhances viability of transplanted hepatocytes and induces angiogenesis

Author:
Yung-Te Hou; Hiroyuki Ijima; Takayuki Takei; Koei Kawakami
Source:
Journal of bioscience and bioengineering 2011 v.112 no.3 pp. 265-272
ISSN:
1389-1723
Subject:
angiogenesis; collagen; foams; gels; hemoglobin; hepatocytes; liver; liver diseases; polyurethanes; rats; tissue engineering; vascular endothelial growth factors; viability
Abstract:
... Hepatocyte transplantation is being explored as a treatment strategy for end-stage liver disease; however, the main limitation is the insufficient vascularization of transplanted hepatocytes. To overcome this problem, a suitable 3D microenvironment and the types of transplanted cells must be considered for hepatocyte transplantation. In this study, a growth factor (GF)/heparin-immobilized collagen ...
DOI:
10.1016/j.jbiosc.2011.05.003

26. Local administration of TGFβ-1/VEGF₁₆₅ gene-transduced bone mesenchymal stem cells for Achilles allograft replacement of the anterior cruciate ligament in rabbits

Author:
Xuelei Wei; Zebin Mao; Yu Hou; Lin Lin; Tao Xue; Lianxu Chen; Haijun Wang; Changlong Yu
Source:
Biochemical and biophysical research communications 2011 v.406 no.2 pp. 204-210
ISSN:
0006-291X
Subject:
allografting; angiogenesis; anterior cruciate ligament; biomechanics; collagen; gene therapy; genes; mechanical properties; rabbits; stem cells; toluidine blue; vascular endothelial growth factors
Abstract:
... Graft remodeling following anterior cruciate ligament (ACL) reconstruction requires a long period of recovery before it is capable of withstanding physiological loads. Graft revascularization is extremely important in the remodeling process. In ACL reconstruction, the local administration of vascular endothelial growth factor (VEGF) significantly increased revascularization of the graft, but did n ...
DOI:
10.1016/j.bbrc.2011.02.015

27. Mechanical and biological properties of keratose biomaterials

Author:
Roche C. de Guzman; Michelle R. Merrill; Jillian R. Richter; Rawad I. Hamzi; Olga K. Greengauz-Roberts; Mark E. Van Dyke
Source:
Biomaterials 2011 v.32 no.32 pp. 8205-8217
ISSN:
0142-9612
Subject:
physical properties; action potentials; gels; sulfonic acids; mass spectrometry; metallic elements; extracellular matrix; peracetic acid; disulfide bonds; keratin; leukocytes; amino acids; oxidation; spinal cord; dialysis; cortex; medicine; humans; resorption; cytotoxicity; collagen; encapsulation; mice; biocompatible materials; amino acid composition; acid treatment; thiols; crosslinking; freeze drying; hydrocolloids; foams; fibroblasts; tissue engineering; angiogenesis; nerve tissue
Abstract:
... The oxidized form of extractable human hair keratin proteins, commonly referred to as keratose, is gaining interest as a biomaterial for multiple tissue engineering studies including those directed toward peripheral nerve, spinal cord, skin, and bone regeneration. Unlike its disulfide cross-linked counterpart, kerateine, keratose does not possess a covalently cross-linked network structure and con ...
DOI:
10.1016/j.biomaterials.2011.07.054

28. Non-RGD-containing snake venom disintegrins, functional and structural relations

Author:
Erin M. Walsh; Cezary Marcinkiewicz
Source:
Toxicon 2011 v.58 no.4 pp. 355-362
ISSN:
0041-0101
Subject:
active sites; amino acids; angiogenesis; apoptosis; asthma; birds; collagen; insulin-dependent diabetes mellitus; integrins; metastasis; models; polypeptides; rodents; snakes; venoms
Abstract:
... Snake venom disintegrins are present in a variety of species and are functionally divided into three families: RGD, MLD and R/KTS. The RGD family of disintegrins, which bind and inhibit the physiological functions of RGD-dependent integrins, constitute the largest and most investigated family. This review will be focused on characterization of two relatively new families of snake venom disintegrin ...
DOI:
10.1016/j.toxicon.2011.07.004

29. Oral administration of marine collagen peptides from Chum Salmon skin enhances cutaneous wound healing and angiogenesis in rats

Author:
Zhaofeng Zhang; Junbo Wang; Ye Ding; Xiaoqian Dai; Yong Li
Source:
Journal of the science of food and agriculture 2011 v.91 no.12 pp. 2173-2179
ISSN:
0022-5142
Subject:
Oncorhynchus keta; angiogenesis; animal injuries; animal models; collagen; excision; oral administration; peptides; rats; tensile strength; tissue repair; wound treatment
Abstract:
... BACKGROUND: A wound is a clinical entity which often poses problems in clinical practice. The present study was aimed to investigate the wound healing potential of administering marine collagen peptides (MCP) from Chum Salmon skin by using two wound models (incision and excision) in rats. RESULTS: Ninety‐six animals were equally divided into the two wound models and then within each model animals ...
DOI:
10.1002/jsfa.4435
PubMed:
21560132

30. Patterns of cancer invasion revealed by QDs-based quantitative multiplexed imaging of tumor microenvironment

Author:
Chun-Wei Peng; Xiu-Li Liu; Chuang Chen; Xiong Liu; Xue-Qin Yang; Dai-Wen Pang; Xiao-Bo Zhu; Yan Li
Source:
Biomaterials 2011 v.32 no.11 pp. 2907-2917
ISSN:
0142-9612
Subject:
angiogenesis; coevolution; collagen; gelatinase B; image analysis; macrophages; neoplasm cells; proteolysis; quantum dots; washing
Abstract:
... Tumor growth and progression depends on their microenvironment, which undergoes constant co-evolution because of the dynamic tumor–stormal interactions. Such co-evolution has long been under appreciated due to the lack of appropriate technology platforms to simultaneously reveal these complex interactions. Here we report on a quantum dots based multiplexed imaging and spectrum analysis technology ...
DOI:
10.1016/j.biomaterials.2010.12.053

31. Potential Activity of Aqueous Extract of Culinary-Medicinal Lion's Mane Mushroom, Hericium erinaceus (Bull.: Fr.) Pers. (Aphyllophoromycetideae) in Accelerating Wound Healing in Rats

Author:
Abdulla, Mahmood Ameen; Fard, Atieh Abdollahi; Sabaratnam, Vikineswary; Wong, Kah-Hui; Kuppusamy, Umah Rani; Abdullah, Noorlidah; Ismail, Salmah
Source:
International journal of medicinal mushrooms 2011 v.13 no.1 pp. 33-39
ISSN:
1521-9437
Subject:
Hericium; angiogenesis; collagen; fruiting bodies; gels; histology; macrophages; males; medicinal fungi; mushrooms; neck; rats; seals; tissue repair; topical application
Abstract:
... This study was conducted to evaluate the effects of topical application of aqueous extract of Hericium erinaceus fruiting bodies (HEFB) on the rate of wound healing enclosure and histology of the healed wound. Five groups of male Sprague-Dawley rats were experimentally wounded in the posterior neck area. A uniform wound area of 2.00 cm in diameter, using a circular stamp, was excised from the nape ...
DOI:
10.1615/IntJMedMushr.v13.i1.50

32. Potential dermal wound healing agent in Blechnum orientale Linn

Author:
How Yee Lai; Yau Yan Lim; Kah Hwi Kim
Source:
BMC complementary and alternative medicine 2011 v.11 no.1 pp. 62
ISSN:
1472-6882
Subject:
Blechnum orientale; abscess; alternative medicine; analysis of variance; angiogenesis; antibacterial properties; antioxidants; bladder; blisters; collagen; cream; excision; ferns and fern allies; fibroblasts; histopathology; hydroxyproline; pain; rats; stomach; synergism; tissue repair
Abstract:
... BACKGROUND: Blechnum orientale Linn. (Blechnaceae) is used ethnomedicinally to treat wounds, boils, blisters or abscesses and sores, stomach pain and urinary bladder complaints. The aim of the study was to validate the ethnotherapeutic claim and to evaluate the effects of B. orientale water extract on wound healing activity. METHODS: Water extract of B. orientale was used. Excision wound healing a ...
DOI:
10.1186/1472-6882-11-62
PubMed:
21835039
PubMed Central:
PMC3170243

33. Quantification of in vitro and in vivo angiogenesis stimulated by ovine forestomach matrix biomaterial

Author:
Sharleen M. Irvine; Juliet Cayzer; Elise M. Todd; Stan Lun; Evan W. Floden; Leonardo Negron; James N. Fisher; Sandi G. Dempsey; Alan Alexander; Michael C. Hill; Annalee O’Rouke; Sarah P. Gunningham; Cameron Knight; Paul F. Davis; Brian R. Ward; Barnaby C.H. May
Source:
Biomaterials 2011 v.32 no.27 pp. 6351-6361
ISSN:
0142-9612
Subject:
angiogenesis; aorta; biomimetics; cell adhesion; chickens; chorioallantoic membrane; collagen; endothelial cells; extracellular matrix; forestomach; humans; in vitro studies; models; sheep; swine; tissue repair
Abstract:
... Ovine forestomach matrix (OFM) biomaterial acts as a biomimetic of native extracellular matrix (ECM) by providing structural and functional cues to orchestrate cell activity during tissue regeneration. The ordered collagen matrix of the biomaterial is supplemented with secondary ECM-associated macromolecules that function in cell adhesion, migration and communication. As angiogenesis and vasculoge ...
DOI:
10.1016/j.biomaterials.2011.05.040

34. Tailoring the porosity and pore size of electrospun synthetic human elastin scaffolds for dermal tissue engineering

Author:
Jelena Rnjak-Kovacina; Steven G. Wise; Zhe Li; Peter K.M. Maitz; Cara J. Young; Yiwei Wang; Anthony S. Weiss
Source:
Biomaterials 2011 v.32 no.28 pp. 6729-6736
ISSN:
0142-9612
Subject:
angiogenesis; cell movement; collagen; elastin; fibroblasts; humans; mice; porosity; tensile strength; tissue engineering
Abstract:
... We obtained low and high porosity synthetic human elastin scaffolds by adapting low (1 mL/h) and high (3 mL/h) flow rates respectively during electrospinning. Physical, mechanical and biological properties of these scaffolds were screened to identify the best candidates for the bioengineering of dermal tissue. SHE scaffolds that were electrospun at the higher flow rate presented increased fiber di ...
DOI:
10.1016/j.biomaterials.2011.05.065

35. Use of Adipose Tissue-Derived Mesenchymal Stem Cells for Experimental Tendinitis Therapy in Equines

Author:
Armando de Mattos Carvalho; Ana Liz Garcia Alves; Patrícia Galvão Gomes de Oliveira; Luis Emiliano Cisneros Álvarez; Renée Laufer Amorim; Carlos Alberto Hussni; Elenice Deffune
Source:
Journal of equine veterinary science 2011 v.31 no.1 pp. 26-34
ISSN:
0737-0806
Subject:
adipose tissue; angiogenesis; athletes; collagen; collagenase; extracellular matrix; histopathology; lameness; mares; models; stem cells; tail; tendinitis; tendons; therapeutics; ultrasonography
Abstract:
... Superficial digital flexor tendon lesion is an important cause of lameness in equine athletes. Although numerous treatments have been described, few are effective at promoting significant improvement in the quality of the extracellular matrix. Therefore, great potential remains for recurrence and in certain cases, an abrupt end to the horse’s athletic career. Recently, several experiments have foc ...
DOI:
10.1016/j.jevs.2010.11.014

36. The biomechanical behavior and host response to porcine-derived small intestine submucosa, pericardium and dermal matrix acellular grafts in a rat abdominal defect model

Author:
Jian Zhang; Guan Yu Wang; Yi Pin Xiao; Lie Ying Fan; Qiang Wang
Source:
Biomaterials 2011 v.32 no.29 pp. 7086-7095
ISSN:
0142-9612
Subject:
adhesion; angiogenesis; animal models; biocompatible materials; biomechanics; collagen; fistula; pericardium; rats; small intestine; surgery; tensile strength; water vapor
Abstract:
... Several porcine-derived acellular biologic grafts are increasingly used in abdominal wall reconstruction due to the limitations of synthetic meshes in many clinical situations. However, relatively little is known so far about their comparative mechanical characteristics and performance after defect repair. We therefore investigated three most commonly used porcine-derived acellular biomaterials, s ...
DOI:
10.1016/j.biomaterials.2011.06.016

37. A mechanism of apigenin-induced apoptosis is potentially related to anti-angiogenesis and anti-migration in human hepatocellular carcinoma cells

Author:
Bo Ra Kim; Young Keul Jeon; Myeong Jin Nam
Source:
Food and chemical toxicology 2011 v.49 no.7 pp. 1626-1632
ISSN:
0278-6915
Subject:
angiogenesis; anticarcinogenic activity; apigenin; apoptosis; cell adhesion; cell movement; collagen; gene expression regulation; hepatoma; humans; messenger RNA; models; protein synthesis; toxicology; two-dimensional gel electrophoresis; vascular endothelial growth factors; vimentin
Abstract:
... Apigenin (APG) has been shown to have a strong anti-cancer effect on various cancer models via a programmed cell death, apoptosis. However, the fundamental mechanisms of these effects are still unclear. In the present study, we examined the question of whether or not APG can inhibit proliferation of hepatocellular carcinoma (HCC), huh-7 cells, resulting in apoptosis. In APG-treated cells, we obser ...
DOI:
10.1016/j.fct.2011.04.015

38. Achyranthes aspera Linn. accelerates cutaneous wound healing in immunocompromised rats by modulating endogenous antioxidants

Author:
C.C. Barua; S.A. Begum; D.C. Pathak; R.S. Bora; A. Gupta
Source:
Indian journal of veterinary pathology 2012 v.36 no.2 pp. 183-186
ISSN:
0973-970X
Subject:
Achyranthes aspera; angiogenesis; animal pathology; antioxidants; ascorbic acid; basement membrane; body weight; catalase; collagen; cortisol; cough; drugs; excision; fibroblasts; histopathology; hydroxyproline; methanol; ointments; pneumonia; topical application
Abstract:
... Achyranthes aspera Linn is traditionally used to treat piles, pneumonia, cough and skin eruptions. The present study was designed to investigate the wound healing activity of 5% ointment of methanol extract of leaves of A. aspera (MEAA) by topical application and the endogenous antioxidant profile in experimental immunocompromised rats with excision wound. Immunocompromised state was induced by pr ...

39. Antiangiogenic and antivascular effects of a recombinant tumstatin-derived peptide in a corneal neovascularization model

Author:
Roman Esipov; Ksenia Beyrakhova; Vera Likhvantseva; Evgenia Stepanova; Vasily Stepanenko; Maria Kostromina; Yulia Abramchik; Anatoly Miroshnikov
Source:
Biochimie 2012 v.94 no.6 pp. 1368-1375
ISSN:
0300-9084
Subject:
Escherichia coli; Tobacco etch virus; angiogenesis; anion exchange; blood vessels; collagen; cornea; dose response; endothelial cells; fibroblasts; ion exchange chromatography; mice; models; proteinases; rabbits; reversed-phase high performance liquid chromatography; viruses
Abstract:
... Tumstatin, a cleavage fragment of collagen IV, is a potent endogenous inhibitor of angiogenesis. Tumstatin-derived peptide T8 possesses all angiostatic properties of full-length tumstatin and indirectly suppresses tumor growth. The potential of T8 to block pathological angiogenesis in the eye has not been explored yet. Here we assess antiangiogenic effects of a recombinant T8 peptide in rabbit cor ...
DOI:
10.1016/j.biochi.2012.03.007

40. Blocking αvβ3 integrin by a recombinant RGD disintegrin impairs VEGF signaling in endothelial cells

Author:
Cyntia F. Montenegro; Carmen L. Salla-Pontes; Juliana U. Ribeiro; Aline Z. Machado; Rafael F. Ramos; Camila C. Figueiredo; Verônica Morandi; Heloisa S. Selistre-de-Araujo
Source:
Biochimie 2012 v.94 no.8 pp. 1812-1820
ISSN:
0300-9084
Subject:
Bothrops; angiogenesis; blood flow; breast neoplasms; cell adhesion; cell movement; clinical trials; collagen; endothelial cells; fibroblasts; integrins; mechanism of action; messenger RNA; metalloproteinases; metastasis; models; neoplasm cells; receptors; snakes; vascular endothelial growth factor receptor-1; vascular endothelial growth factor receptor-2; vascular endothelial growth factors; venoms
Abstract:
... Vascular endothelial growth factor (VEGF) and αvβ3 integrin are key molecules that actively participate in tumor angiogenesis and metastasis. Some integrin-blocking molecules are currently under clinical trials for cancer and metastasis treatment. However, the mechanism of action of such inhibitors is not completely understood. We have previously demonstrated the anti-angiogenic and anti-metastati ...
DOI:
10.1016/j.biochi.2012.04.020

41. Development of growth factor-immobilizable material for hepatocyte transplantation

Author:
Yung-Te Hou; Hiroyuki Ijima; Nana Shirakigawa; Takayuki Takei; Koei Kawakami
Source:
Biochemical engineering journal 2012 v.69 no. pp. 172-181
ISSN:
1369-703X
Subject:
angiogenesis; cell viability; collagen; foams; gels; growth and development; hemoglobin; heparin; hepatocytes; liver; polyurethanes; tissue engineering
Abstract:
... Growth factor (GF)-immobilizable materials were developed as a practical hepatocyte transplantation method for reconstructing a tissue-like structure in liver tissue engineering. Two GF-immobilizable scaffolds, namely single hepatocyte-embedded, heparin-immobilized, collagen-gel-filled polyurethane foam, and hepatocyte spheroid-embedded, heparin-immobilized, collagen-gel-filled polyurethane foam w ...
DOI:
10.1016/j.bej.2012.09.007

42. Dexamethasone reduces bronchial wall remodeling during pulmonary migration of Strongyloides venezuelensis larvae in rats

Author:
Cristiane Tefé-Silva; Cristina T. Beneli; Mara R. Celes; Eleuza R. Machado; Marlene T. Ueta; Carlos A. Sorgi; Elaine M. Floriano; Lúcia H. Faccioli; Simone G. Ramos
Source:
Parasitology international 2012 v.61 no.3 pp. 425-430
ISSN:
1383-5769
Subject:
Strongyloides; angiogenesis; asthma; collagen; dexamethasone; edema; enzyme-linked immunosorbent assay; fibrosis; hyperplasia; immune response; immunohistochemistry; interleukin-4; larvae; morphometry; parasitology; rats; secretion; smooth muscle; strongyloidiasis; vascular endothelial growth factors
Abstract:
... Strongyloidiasis is an intestinal parasitosis with an obligatory pulmonary cycle. A Th2-type immune response is induced and amplifies the cellular response through the secretion of inflammatory mediators. Although this response has been described as being similar to asthma, airway remodeling during pulmonary migration of larvae has not yet been established. The aim of this study was to identify th ...
DOI:
10.1016/j.parint.2012.02.004

43. Doping control analysis of TB-500, a synthetic version of an active region of thymosin β₄, in equine urine and plasma by liquid chromatography–mass spectrometry

Author:
Emmie N.M. Ho; W.H. Kwok; M.Y. Lau; April S.Y. Wong; Terence S.M. Wan; Kenneth K.H. Lam; Peter J. Schiff; Brian D. Stewart
Source:
Journal of chromatography 2012 v.1265 no. pp. 57-69
ISSN:
0021-9673
Subject:
acetylation; actin; active sites; angiogenesis; cell differentiation; cell movement; collagen; endothelial cells; horses; in vitro studies; inflammation; ingredients; ion exchange; ions; keratinocytes; liquid chromatography; mass spectrometry; metabolites; solid phase extraction; sports; thymosin; tissue repair; tissues; urine
Abstract:
... A veterinary preparation known as TB-500 and containing a synthetic version of the naturally occurring peptide LKKTETQ has emerged. The peptide segment ¹⁷LKKTETQ²³ is the active site within the protein thymosin β₄ responsible for actin binding, cell migration and wound healing. The key ingredient of TB-500 is the peptide LKKTETQ with artificial acetylation of the N-terminus. TB-500 is claimed to p ...
DOI:
10.1016/j.chroma.2012.09.043

44. Effect of Andrographis paniculata leaf extract on wound healing in rats

Author:
Fouad Hussain Al-Bayaty; Mahmood Ameen Abdulla; Mohamed Ibrahim Abu Hassan; Hapipah Mohd Ali
Source:
Natural product research 2012 v.26 no.5 pp. 423-429
ISSN:
1478-6427
Subject:
Andrographis paniculata; angiogenesis; collagen; fibroblasts; gels; leaf extracts; neck; rats; tissue repair; topical application
Abstract:
... This work was carried out to study the effect of topical application of Andrographis paniculata on the rate of wound enclosure and its histological features. A wound was created in four groups of rat in posterior neck region. Blank placebo was applied topically to the wounds of Group 1. Groups 2 and 3 were dressed with placebo containing 5% and 10% extracts of A. paniculata, respectively. Intrasit ...
DOI:
10.1080/14786419.2010.496114

45. Flt1 and Flk1 mediate regulation of intraocular pressure and their double heterozygosity causes the buphthalmia in mice

Author:
Keigo Sano; Osamu Katsuta; Satoshi Shirae; Yoshiaki Kubota; Masatsugu Ema; Toshio Suda; Masatsugu Nakamura; Masanori Hirashima
Source:
Biochemical and biophysical research communications 2012 v.420 no.2 pp. 422-427
ISSN:
0006-291X
Subject:
angiogenesis; cell death; collagen; eyes; ganglia; genes; glaucoma; heterozygosity; humans; mice; mutants; receptor protein-tyrosine kinase; tissues; vascular endothelial growth factor A
Abstract:
... Flt1 and Flk1 are receptor tyrosine kinases for vascular endothelial growth factor-A which play a crucial role in physiological and pathological angiogenesis. To study genetic interaction between the Flt1 and Flk1 genes, we crossed between Flt1 and Flk1 heterozygous (Flt1⁺/⁻ and Flk1⁺/⁻) mice. We found that Flt1; Flk1 double heterozygous (Flt1⁺/⁻; Flk1⁺/⁻) mice showed enlarged eyes similar to the ...
DOI:
10.1016/j.bbrc.2012.03.011

46. Humanin prevents intra-renal microvascular remodeling and inflammation in hypercholesterolemic ApoE deficient mice

Author:
Xin Zhang; Victor H. Urbieta-Caceres; Alfonso Eirin; Caitlin C. Bell; John A. Crane; Hui Tang; Kyra L. Jordan; Yun-Kyu Oh; Xiang-Yang Zhu; Michael J. Korsmo; Adi R. Bachar; Pinchas Cohen; Amir Lerman; Lilach O. Lerman
Source:
Life sciences 2012 v.91 no.5-6 pp. 199-206
ISSN:
0024-3205
Subject:
Western blotting; angiogenesis; angiopoietin-1; apoptosis; atherogenesis; atherosclerosis; collagen; diet; fibrosis; histology; humans; hypercholesterolemia; inflammation; kidney diseases; kidneys; mice; osteopontin; protective effect; tumor necrosis factor-alpha
Abstract:
... AIMS: Humanin (HN) is an endogenous mitochondrial-derived cytoprotective peptide that has shown protective effects against atherosclerosis and is expressed in human vessels. However, its effects on the progression of kidney disease are unknown. We hypothesized that HN would protect the kidney in the early phase of atherogenesis. MAIN METHODS: Forty-eight mice were studied in four groups (n=12 each ...
DOI:
10.1016/j.lfs.2012.07.010
PubMed:
22820173
PubMed Central:
PMC3703734

47. In vitro microvessels for the study of angiogenesis and thrombosis

Author:
Ying Zheng; Junmei Chen; Michael Craven; Nak Won Choi; Samuel Totorica; Anthony Diaz-Santana; Pouneh Kermani; Barbara Hempstead; Claudia Fischbach-Teschl; José A. López; Abraham D. Stroock
Source:
Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.24 pp. 9342-9347
ISSN:
0027-8424
Subject:
angiogenesis; collagen; endothelium; inflammation; mass transfer; pathophysiology; thrombosis; tissue scaffolds
Abstract:
... Microvascular networks support metabolic activity and define microenvironmental conditions within tissues in health and pathology. Recapitulation of functional microvascular structures in vitro could provide a platform for the study of complex vascular phenomena, including angiogenesis and thrombosis. We have engineered living microvascular networks in three-dimensional tissue scaffolds and demons ...
DOI:
10.1073/pnas.1201240109
PubMed:
22645376
PubMed Central:
PMC3386137

48. Inhibition of MT1-MMP activity using functional antibody fragments selected against its hemopexin domain

Author:
B. Basu; P. Correa de Sampaio; H. Mohammed; M. Fogarasi; P. Corrie; N.A. Watkins; P.A. Smethurst; W.R. English; W.H. Ouwehand; G. Murphy
Source:
international journal of biochemistry & cell biology 2012 v.44 no.2 pp. 393-403
ISSN:
1357-2725
Subject:
humans; neoplasms; antibodies; proteinases; therapeutics; collagen; neoplasm cells; active sites; angiogenesis
Abstract:
... The membrane associated MMP, MT1-MMP, is a critical pericellular protease involved in tumour cell invasion and angiogenesis and is highly up-regulated in numerous human cancers. It therefore represents an exciting new therapeutic cancer-specific target. We have generated recombinant human scFv antibodies against the non-catalytic, hemopexin domain of MT1-MMP that modulate its interactions with col ...
DOI:
10.1016/j.biocel.2011.11.015

49. Inhibitory effects of salvianolic acid B on CCl₄-induced hepatic fibrosis through regulating NF-κB/IκBα signaling

Author:
Rong Wang; Xiao-Yan Yu; Zhu-Ying Guo; Yu-Jie Wang; Yan Wu; Yong-Fang Yuan
Source:
Journal of ethnopharmacology 2012 v.144 no.3 pp. 592-598
ISSN:
0378-8741
Subject:
Salvia miltiorrhiza; Western blotting; alanine transaminase; angiogenesis; aspartate transaminase; bilirubin; body weight; carbon tetrachloride; cell nucleolus; collagen; cytoplasm; fibrosis; hyaluronic acid; laminin; liver; liver cirrhosis; models; patients; radioimmunoassays; rats; salvianolic acid
Abstract:
... ETHNOPHARMACOLOGICAL RELEVANCE: Hepatic fibrosis, a precursor of liver cirrhosis, is a consequence of severe liver damage that occurs in many patients with chronic liver diseases. Salvianolic acid B (SA-B) is one of water soluble compounds derived from Salvia miltiorrhiza Bunge (Danshen in Chinese) widely used for chronic liver diseases. In this study we investigated the protective effects of SA-B ...
DOI:
10.1016/j.jep.2012.09.048

50. Injectable system for spatio-temporally controlled delivery of hypoxia-induced angiogenic signalling

Author:
E. Hadjipanayi; U. Cheema; U. Hopfner; A. Bauer; H.G. Machens; A.F. Schilling
Source:
Journal of controlled release 2012 v.161 no.3 pp. 852-860
ISSN:
0168-3659
Subject:
angiogenesis; bioactive properties; collagen; hypoxia; interleukin-8; oxygen; phase transition; temperature; tissues; vascular endothelial growth factors
Abstract:
... While chronically ischaemic tissues are continuously exposed to hypoxia, the primary angiogenic stimulus, they fail to appropriately respond to it, as hypoxia-regulated angiogenic factor production gradually undergoes down-regulation, thus hindering adaptive angiogenesis. We have previously reported on two strategies for delivering on demand hypoxia-induced signalling (HIS) in vivo, namely, implan ...
DOI:
10.1016/j.jconrel.2012.04.048