Subject term: animal ovaries and cell lines

1. β1,6 N-Acetylglucosaminyltransferase (core 2 GlcNAc-T) expression in normal rat tissues and different cell lines: Evidence for complex mechanisms of regulation

Author:: Ingrid E. VanderElst; Alessandro Datti
Source:: Glycobiology 1998 v.8 no.7 pp. 731-740
ISSN:: 1460-2423
Subject:: Chinese hamsters; animal ovaries; blood serum; butyrates; cell lines; cholera toxin; endoderm; enzyme activity; fluorescence; heart; liver; lungs; messenger RNA; polyclonal antibodies; protein content; protein synthesis; proteolysis; rats; reverse transcriptase polymerase chain reaction; spleen; tissues; transferases
Abstract:: ... The distribution of the Golgi enzyme β1,6-N-acetylglucosaminyltransferase (core 2 GlcNAc-T for short) has been investigated in several tissue and cell systems by combining the potentials of a polyclonal antibody and a novel, sensitive fluorescent enzyme assay. In normal rat tissues, levels of the protein were found to vary and as a general trend did not correlate with enzyme activities. Additional ...
DOI:: 10.1093/glycob/8.7.731
: https://doi.org/10.1093/glycob/8.7.731

2. Oligosaccharide analysis and molecular modeling of soluble forms of glycoproteins belonging to the Ly-6, scavenger receptor, and immunoglobulin superfamilies expressed in Chinese hamster ovary cells

Author:: Pauline M. Rudd3; Mark R. Wormald; David J. Harvey; Mercy Devasahayam; Mark S.B. McAlister; Marion H. Brown; Simon J. Davis; A.Neil Barclay; Raymond A. Dwek
Source:: Glycobiology 1999 v.9 no.5 pp. 443-458
ISSN:: 1460-2423
Subject:: Chinese hamsters; active sites; amino acid sequences; animal ovaries; antigens; cell lines; cell membranes; databases; glycoproteins; immunoglobulins; leukocytes; molecular models; oligosaccharides; polysaccharides; protein-protein interactions
Abstract:: ... Most cell surface molecules are glycoproteins consisting of linear arrays of globular domains containing stretches of amino acid sequence with similarities to regions in other proteins. These conserved regions form the basis for the classification of proteins into superfamilies. Recombinant soluble forms of six leukocyte antigens belonging to the Ly-6 (CD59), scavenger receptor (CD5), and immunogl ...
DOI:: 10.1093/glycob/9.5.443
: https://doi.org/10.1093/glycob/9.5.443

3. Selection strategies for the establishment of recombinant Chinese hamster ovary cell line with dihydrofolate reductase-mediated gene amplification

Author:: Seung Chul Jun; Min Soo Kim; Jong Youn Baik; Sun Ok Hwang; Gyun Min Lee
Source:: Applied microbiology and biotechnology 2005 v.69 no.2 pp. 162-169
ISSN:: 0175-7598
Subject:: Chinese hamsters; animal ovaries; antibodies; cell lines; clones; cloning (cells); gene amplification
Abstract:: ... To evaluate the efficacy of selection strategies for recombinant Chinese hamster ovary (rCHO) clones undergone with dihydrofolate reductase-mediated gene amplification, rCHO cell lines producing a chimeric antibody were established using two strategies, one based on individual clones and the other based on cell pools. In a selection based on individual clones, cell cloning by limiting dilution met ...
DOI:: 10.1007/s00253-005-1972-8
: https://doi.org/10.1007/s00253-005-1972-8

4. Adhesion and migration of ovarian cancer cells on crosslinked laminin fibers nanofabricated by multiphoton excited photochemistry

Author:: Xiyi Chen; Molly A. Brewer; Changping Zou; Paul J. Campagnola
Source:: Integrative biology 2009 v.1 no.7 pp. 469-476
ISSN:: 1757-9708
Subject:: adhesion; animal ovaries; cell lines; cell polarity; collagen; crosslinking; epithelial cells; focal adhesions; gels; laminin; metastasis; models; nanofibers; neoplasm cells; ovarian neoplasms; photochemistry
Abstract:: ... Ovarian cancer is the deadliest gynecological cancer, which may arise in part due to the concurrent invasion and metastasis of high grade tumors. It is thus crucial to gain insight into the adhesion and migration mechanisms in vivo, as this may ultimately lead to new treatment/detection options. To explore this possibility, we have used multiphoton excited photochemistry (MPE) to synthesize models ...
DOI:: 10.1039/b906310b
PubMed:: 20023757
PubMed Central:: PMC4337955
: https://doi.org/10.1039/b906310b

5. Platinum(ii) compounds bearing bone-targeting group: synthesis, crystal structure and antitumor activity

Author:: Zuqin Xue; Miaoxin Lin; Jianhui Zhu; Junfeng Zhang; Yizhi Li; Zijian Guo
Source:: Chemical communications 2010 v.46 no.8 pp. 1212-1214
ISSN:: 1364-548X
Subject:: animal ovaries; antineoplastic activity; apoptosis; aqueous solutions; cell lines; chemical communication; chemical reactions; cisplatin; crystal structure; cytotoxicity; humans; moieties; osteosarcoma; ovarian neoplasms; phosphonates; platinum; solubility
Abstract:: ... Platinum(ii) complexes bearing geminal bisphosphonate moieties have excellent solubility in both organic and aqueous solutions and show considerable cytotoxicity against human osteosarcoma (MG-63) and ovarian cancer (COC1) cell lines with different apoptotic pathways from that of cisplatin. ...
DOI:: 10.1039/b922222g
: https://doi.org/10.1039/b922222g

6. Conformational and thermal stability of mature dimeric human myeloperoxidase and a recombinant monomeric form from CHO cells

Author:: Srijib Banerjee; Johanna Stampler; Paul G. Furtmüller; Christian Obinger
Source:: Biochimica et biophysica acta 2011 v.1814 no.2 pp. 375-387
ISSN:: 1878-1454
Subject:: Chinese hamsters; animal ovaries; anti-infective properties; biosynthesis; bone marrow; cell lines; cytoplasmic granules; denaturation; differential scanning calorimetry; dithiothreitol; enzymology; fluorescence emission spectroscopy; heme; humans; innate immunity; monocytes; myeloperoxidase; neutrophils; polypeptides; post-translational modification; thermal stability
Abstract:: ... Myeloperoxidase (MPO) is a lysosomal heme enzyme present in the azurophilic granules of human neutrophils and monocytes. It is a critical element of the human innate immune system by exerting antimicrobial effects. It is a disulfide bridged dimer with each monomer containing a light and a heavy polypeptide and its biosynthesis and intracellular transport includes several posttranslational processi ...
DOI:: 10.1016/j.bbapap.2010.09.015
: https://doi.org/10.1016/j.bbapap.2010.09.015

7. Efficient and reproducible mammalian cell bioprocesses without probes and controllers?

Author:: Stéphanie Tissot; Agata Oberbek; Martino Reclari; Matthieu Dreyer; David L. Hacker; Lucia Baldi; Mohamed Farhat; Florian M. Wurm
Source:: New biotechnology 2011 v.28 no.4 pp. 382-390
ISSN:: 1871-6784
Subject:: Chinese hamsters; animal ovaries; bioprocessing; bioreactors; carbon dioxide; cell lines; cell viability; chemical concentration; controllers; culture media; dissolved oxygen; liquid state fermentation; mass transfer; mixing; monoclonal antibodies; oxygen; pH; receptors; recombinant proteins; redox potential; tumor necrosis factors
Abstract:: ... Bioprocesses for recombinant protein production with mammalian cells are typically controlled for several physicochemical parameters including the pH and dissolved oxygen concentration (DO) of the culture medium. Here we studied whether these controls are necessary for efficient and reproducible bioprocesses in an orbitally shaken bioreactor (OSR). Mixing, gas transfer, and volumetric power consum ...
DOI:: 10.1016/j.nbt.2011.02.004
: https://doi.org/10.1016/j.nbt.2011.02.004

8. LSINCT5 is over expressed in breast and ovarian cancer and affects cellular proliferation

Author:: Jessica M. Silva; Nicole J. Boczek; Michael W. Berres; Xinghong Ma; David I. Smith
Source:: RNA biology 2011 v.8 no.3 pp. 496-505
ISSN:: 1555-8584
Subject:: DNA-directed RNA polymerase; animal ovaries; cell lines; cell proliferation; gene expression regulation; genes; humans; messenger RNA; microRNA; neoplasm cells; non-coding RNA; ovarian neoplasms; quantitative polymerase chain reaction; tissues; transcription (genetics)
Abstract:: ... More than 98% of the human genome is comprised of non-protein coding sequences. Interestingly, a considerable fraction of these sequences is transcribed into non-protein coding RNA transcripts. These transcripts range in size from very small RNAs such as the miRNAs (20–25 base pairs) to transcripts that can range up to 100 kb or more. Some longer non-coding RNAs (lncRNAs) have been found to play i ...
DOI:: 10.4161/rna.8.3.14800
: https://doi.org/10.4161/rna.8.3.14800

9. Computational identification of microRNA gene loci and precursor microRNA sequences in CHO cell lines

Author:: Matthias Hackl; Vaibhav Jadhav; Tobias Jakobi; Oliver Rupp; Karina Brinkrolf; Alexander Goesmann; Alfred Pühler; Thomas Noll; Nicole Borth; Johannes Grillari
Source:: Journal of biotechnology 2012 v.158 no.3 pp. 151-155
ISSN:: 0168-1656
Subject:: Chinese hamsters; DNA; animal ovaries; bioinformatics; biomarkers; biotechnology; cell culture; cell engineering; cell lines; chromosome mapping; gene overexpression; genes; genomics; loci; microRNA; microarray technology; nucleotide sequences; phenotype; polymerase chain reaction; sequence alignment
Abstract:: ... MicroRNAs (miRNAs) have recently entered Chinese hamster ovary (CHO) cell culture technology, due to their severe impact on the regulation of cellular phenotypes. Applications of miRNAs that are envisioned range from biomarkers of favorable phenotypes to cell engineering targets. These applications, however, require a profound knowledge of miRNA sequences and their genomic organization, which exce ...
DOI:: 10.1016/j.jbiotec.2012.01.019
PubMed:: 22306111
PubMed Central:: PMC3314935
: https://doi.org/10.1016/j.jbiotec.2012.01.019

10. Identifying the CHO Secretome using Mucin-type O-Linked Glycosylation and Click-chemistry

Author:: Slade Peter G.; Hajivandi Mahbod; Bartel Cheryl Moody; Gorfien Stephen F.
Source:: Journal of Proteome Research 2012 v.11 no.12 pp. 6175-6186
ISSN:: 1535-3907
Subject:: Chinese hamsters; animal ovaries; biopharmaceuticals; cell culture; cell lines; culture media; glycosylation; mass spectrometry; polysaccharides; protein secretion; proteome; recombinant proteins
Abstract:: ... Chinese hamster ovary cells (CHO) are the most common cell line used in the production of therapeutic proteins. Understanding the complex pattern of secreted host cell proteins (HCP) that are released by CHO cells will facilitate the development of new recombinant protein production processes. In this study, we have adapted the N-azido-galactosamine (GalNAz) metabolic labeling method to enable the ...
DOI:: 10.1021/pr300810f
: https://doi.org/10.1021/pr300810f

11. Characterization of Host-Cell Line Specific Glycosylation Profiles of Early Transmitted/Founder HIV-1 gp120 Envelope Proteins

Author:: Go Eden P.; Liao Hua-Xin; Alam S. Munir; Hua David; Haynes Barton F.; Desaire Heather
Source:: Journal of Proteome Research 2013 v.12 no.3 pp. 1223-1234
ISSN:: 1535-3907
Subject:: Chinese hamsters; Human immunodeficiency virus 1; animal ovaries; antigens; cell lines; dissociation; electron transfer; glycopeptides; glycoproteins; glycosylation; humans; immune response; kidneys; mannose; medicinal properties; polysaccharides; proteome; recombinant vaccines; viruses
Abstract:: ... Glycosylation plays an essential role in regulating protein function by modulating biological, structural, and therapeutic properties. However, due to its inherent heterogeneity and diversity, the comprehensive analysis of protein glycosylation remains a challenge. As part of our continuing effort in the analysis of glycosylation profiles of recombinant HIV-1 envelope-based immunogens, we evaluate ...
DOI:: 10.1021/pr300870t
PubMed:: 23339644
PubMed Central:: PMC3674872
: https://doi.org/10.1021/pr300870t

12. Identification of Autocrine Growth Factors Secreted by CHO Cells for Applications in Single-Cell Cloning Media

Author:: Lim U Ming; Yap Miranda Gek Sim; Lim Yoon Pin; Goh Lin-Tang; Ng Say Kong
Source:: Journal of Proteome Research 2013 v.12 no.7 pp. 3496-3510
ISSN:: 1535-3907
Subject:: Chinese hamsters; animal ovaries; autocrine signaling; cell culture; cell lines; fetal bovine serum; growth factors; mass spectrometry; monoclonal antibodies; protein secretion; proteome; proteomics; recombinant proteins; risk reduction
Abstract:: ... Chinese hamster ovary (CHO) cell lines are widely used for the expression of therapeutic recombinant proteins, including monoclonal antibodies and other biologics. For manufacturing, cells derived from a single-cell clone are typically used to ensure product consistency. Presently, fetal bovine serum (FBS) is commonly used to support low cell density cultures to obtain clonal cell populations beca ...
DOI:: 10.1021/pr400352n
: https://doi.org/10.1021/pr400352n

13. Secretome-Based Identification of TFPI2, A Novel Serum Biomarker for Detection of Ovarian Clear Cell Adenocarcinoma

Author:: Arakawa Noriaki; Miyagi Etsuko; Nomura Ayako; Morita Erina; Ino Yoko; Ohtake Norihisa; Miyagi Yohei; Hirahara Fumiki; Hirano Hisashi
Source:: Journal of Proteome Research 2013 v.12 no.10 pp. 4340-4350
ISSN:: 1535-3907
Subject:: Western blotting; animal ovaries; bioinformatics; biomarkers; blood serum; cell lines; enzyme-linked immunosorbent assay; epithelium; graphs; mass spectrometry; monitoring; ovarian neoplasms; patients; prognosis; proteinase inhibitors; proteins; proteome; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; serine proteinases; women
Abstract:: ... Of all of the epithelial ovarian cancers (EOC), clear cell adenocarcinoma (CCA) has the worst clinical prognosis. Furthermore, the conventional EOC biomarker CA125 is more often negative in CCA than in other subtypes of EOC. This study sought to discover a new diagnostic biomarker that would allow more reliable detection of CCA. Using mass spectrometry, we compared proteins in conditioned media fr ...
DOI:: 10.1021/pr400282j
: https://doi.org/10.1021/pr400282j

14. Evaluation of anti-inflammatory and mechanism of action of extract of Macrosiphonia longiflora (Desf.) Müll. Arg

Author:: Anísio Onório da Silva; Aurea Damaceno Alves; Danielle Ayr Tavares de Almeida; Sikiru Olaitan Balogun; Ruberlei Godinho de Oliveira; Aline Aires Aguiar; Ilsamar Mendes Soares; Poliana Guerino Marson-Ascêncio; Sérgio Donizeti Ascêncio; Domingos Tabajara de Oliveira Martins
Source:: Journal of ethnopharmacology 2014 v.154 no.2 pp. 319-329
ISSN:: 0378-8741
Subject:: Apocynaceae; Chinese hamsters; alkaloids; animal ovaries; anti-inflammatory activity; anti-inflammatory agents; carrageenan; cell lines; cell movement; cerrado; cytotoxicity; dextran; edema; ellagic acid; enzyme-linked immunosorbent assay; epithelial cells; high performance liquid chromatography; inflammation; interferon-gamma; interleukin-10; interleukin-17; interleukin-1beta; lipopolysaccharides; luteolin; maceration; mechanism of action; mice; models; myricetin; naringin; neutrophils; nitric oxide; nitrites; peritonitis; phenols; pleurisy; quercetin; rats; rutin; secondary metabolites; spectral analysis; spectroscopy; terpenoids; traditional medicine; tumor necrosis factor-alpha
Abstract:: ... Macrosiphonia longiflora (Desf.) Müll. Arg (Apocynaceae), popularly known as ‘velame’ and ‘velame branco’, is a native subshrub that grows in the Brazilian Cerrado. This plant is widely used in traditional medicine in the form of decoction and infusion, particularly as anti-inflammatory, depurative, anti-rheumatic, antisyphilitic and antiulcer remedy. There is no available information in the liter ...
DOI:: 10.1016/j.jep.2014.03.017
: https://doi.org/10.1016/j.jep.2014.03.017

15. Peptides derived from central turn motifs within integrin αIIb and αV cytoplasmic tails inhibit integrin activation

Author:: Xinlei Li; Yongqing Liu; Thomas A. Haas
Source:: Peptides 2014 v.62 pp. 38-48
ISSN:: 0196-9781
Subject:: Chinese hamsters; adhesion; animal ovaries; breast neoplasms; cell adhesion; cell lines; cytoskeleton; fibrinogen; integrins; ligands; models; neoplasm cells; peptides; tubulin
Abstract:: ... We previously found that peptides derived from the full length of integrin αIIb and αV cytoplasmic tails inhibited their parent integrin activation, respectively. Here we showed that the cell-permeable peptides corresponding to the conserved central turn motif within αIIb and αV cytoplasmic tails, myr-KRNRPPLEED (αIIb peptide) and myr-KRVRPPQEEQ (αV peptide), similarly inhibited both αIIb and αV i ...
DOI:: 10.1016/j.peptides.2014.07.029
: https://doi.org/10.1016/j.peptides.2014.07.029

16. Promoting cell adhesion on slippery phosphorylcholine hydrogel surfaces

Author:: Samantha McRae Page; Sangram Parelkar; Alex Gerasimenko; Dong Yeop Shin; Shelly R. Peyton; Todd Emrick
Source:: Journal of materials chemistry B 2014 v.2 no.6 pp. 620-624
ISSN:: 2050-7518
Subject:: animal ovaries; biocompatibility; cell adhesion; cell lines; cell membranes; copolymerization; gel chromatography; gelation; humans; hydrogels; hydrophilicity; lipoic acid; mice; myoblasts; nuclear magnetic resonance spectroscopy; oligopeptides; ovarian neoplasms; pH; phospholipids; phosphorylcholine; skeletal muscle
Abstract:: ... The growing interest in regenerative medicine has created a need for superior polymer matrices that suit multiple physical, mechanical, and biological requirements. While the phospholipid bilayer of a cell membrane is considered optimal for interacting with biologics, polymeric materials composed of 2-methacryloyloxyethyl phosphorylcholine (MPC) offer a cell membrane-like synthetic alternative. In ...
DOI:: 10.1039/c3tb21493a
: https://doi.org/10.1039/c3tb21493a

17. Synthesis and Anticancer Evaluation of Some New 4-Amino-3- (p-methoxybenzyl)-4,5-dihydro-1,2,4-triazole-5-one Derivatives

Author:: Olcay Bekircan; Murat Kucuk; Bahittin Kahveci; Hakan Bektas
Source:: Zeitschrift für Naturforschung 2014 v.63 no.11 pp. 1305-1314
ISSN:: 0932-0776
Subject:: aldehydes; animal ovaries; antineoplastic activity; breasts; cell lines; central nervous system; colon; condensation reactions; hydrazides; kidneys; leukemia; lungs; melanoma; organobromine compounds; prostate gland; sodium hydroxide; synthesis
Abstract:: ... 4-Amino-3-p-methoxybenzyl-5-oxo-4,5-dihydro-1,2,4-triazol-1-yl-acetic acid ethyl ester (2) was prepared from 4-amino-4,5-dihydro-1H-1,2,4-triazole-5-one (1) and ethyl bromoacetate. Compound 3 was synthesized by the condensation of 2 with hydrazine hydrate. The treatment of compound 3 with various aromatic aldehydes resulted in the formation of arylidene hydrazides as cistrans conformers 4a - g. Th ...
DOI:: 10.1515/znb-2008-1109
: https://doi.org/10.1515/znb-2008-1109

18. Comparison of SYBR Green and TaqMan real-time PCR methods for quantitative detection of residual CHO host-cell DNA in biopharmaceuticals

Author:: Mohammad Soltany-Rezaee-Rad; Zargham Sepehrizadeh; Negar Mottaghi-Dastjerdi; Mojtaba Tabatabaei Yazdi; Neda Seyatesh
Source:: Biologicals 2015 v.43 pp. 130-135
ISSN:: 1045-1056
Subject:: Chinese hamsters; DNA; animal ovaries; biopharmaceuticals; cell lines; detection limit; hosts; industry; melting; oncogenes; quantitative analysis; quantitative polymerase chain reaction; tumor suppressor genes
Abstract:: ... The Chinese hamster ovary (CHO) cell line is one of the predominant hosts used in the bioproduction of pharmaceutical proteins. There have been many concerns about the use of animal cell lines in biopharm industries, and one of the most important concerns has been residual host-cell DNA. Improper integration of residual DNA into the recipient genomes could activate oncogenes or deactivate tumor su ...
DOI:: 10.1016/j.biologicals.2014.11.004
: https://doi.org/10.1016/j.biologicals.2014.11.004

19. Conducting polymer nanoparticles for targeted cancer therapy

Author:: Mona Doshi; Marissa Krienke; Saeid Khederzadeh; Henry Sanchez; Alicja Copik; Jeremiah Oyer; Andre J. Gesquiere
Source:: RSC advances 2015 v.5 no.47 pp. 37943-37956
ISSN:: 2046-2069
Subject:: absorbance; absorption; animal ovaries; biocompatibility; cancer therapy; carboxylic acids; cell lines; cell viability; ethylene oxide; flow cytometry; folate receptors; folic acid; hydrophobicity; lung neoplasms; moieties; nanoparticles; neoplasm cells; ovarian neoplasms; oxygen; photochemotherapy; photosensitizing agents; polystyrenes; singlet oxygen; toxicity; zeta potential
Abstract:: ... First and second generation photosensitizers used in photodynamic therapy (PDT) have shown promising results in clinical applications, aided by recent improvements in light absorption efficiency and quantum yield of singlet oxygen formation. However, these photosensitizers still have several drawbacks that prevent PDT from being an efficient therapy, including lack of selectivity to diseased tissu ...
DOI:: 10.1039/c5ra05125h
: https://doi.org/10.1039/c5ra05125h

20. Elucidation of the CHO Super-Ome (CHO-SO) by Proteoinformatics

Author:: Kumar Amit; Baycin-Hizal Deniz; Wolozny Daniel; Pedersen Lasse Ebdrup; Lewis Nathan E.; Heffner Kelley; Chaerkady Raghothama; Cole Robert N.; Shiloach Joseph; Zhang Hui; Bowen Michael A.; Betenbaugh Michael J.
Source:: Journal of Proteome Research 2015 v.14 no.11 pp. 4687-4703
ISSN:: 1535-3907
Subject:: Chinese hamsters; Internet; animal ovaries; bioinformatics; catalytic activity; cell growth; cell lines; cytoskeleton; databases; drugs; exosomes; extracellular space; immune response; manufacturing; monoclonal antibodies; protein degradation; proteins; proteome; proteomics; purification methods; signal peptide
Abstract:: ... Chinese hamster ovary (CHO) cells are the preferred host cell line for manufacturing a variety of complex biotherapeutic drugs including monoclonal antibodies. We performed a proteomics and bioinformatics analysis on the spent medium from adherent CHO cells. Supernatant from CHO-K1 culture was collected and subjected to in-solution digestion followed by LC/LC–MS/MS analysis, which allowed the iden ...
DOI:: 10.1021/acs.jproteome.5b00588
PubMed:: 26418914
PubMed Central:: PMC4721571
: https://doi.org/10.1021/acs.jproteome.5b00588

21. Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells

Author:: Christine Lin Chin; Hing Kah Chin; Cara Sze Hui Chin; Ethan Tingfeng Lai; Say Kong Ng
Source:: BMC biotechnology 2015 v.15 no.1 pp. 44
ISSN:: 1472-6750
Subject:: Chinese hamsters; animal ovaries; blood serum; cell lines; culture flasks; dihydrofolate reductase; engineering; gene amplification; genes; genetic vectors; interferon-gamma; internal ribosome entry sites; messenger RNA; methotrexate; mice; ornithine decarboxylase; point mutation; protein content; protein degradation; protein synthesis; recombinant proteins; therapeutics; transcription (genetics); translation (genetics)
Abstract:: ... BACKGROUND: Expression vector engineering technology is one of the most convenient and timely method for cell line development to meet the rising demand of novel production cell line with high productivity. Destabilization of dihydrofolate reductase (dhfr) selection marker by addition of AU-rich elements and murine ornithine decarboxylase PEST region was previously shown to improve the specific pr ...
DOI:: 10.1186/s12896-015-0145-9
PubMed:: 26033090
PubMed Central:: PMC4450478
: https://doi.org/10.1186/s12896-015-0145-9

22. High-Antibody-Producing Chinese Hamster Ovary Cells Up-Regulate Intracellular Protein Transport and Glutathione Synthesis

Author:: Orellana Camila A.; Marcellin Esteban; Schulz Benjamin L.; Nouwens Amanda S.; Gray Peter P.; Nielsen Lars K.
Source:: Journal of Proteome Research 2015 v.14 no.2 pp. 609-618
ISSN:: 1535-3907
Subject:: Chinese hamsters; DNA replication; animal ovaries; biosynthesis; cell lines; gene expression regulation; glutathione; monoclonal antibodies; nucleotide sequences; post-translational modification; protein transport; proteins; proteome; proteomics; transfection
Abstract:: ... Chinese hamster ovary (CHO) cells are the preferred production host for therapeutic monoclonal antibodies (mAb) due to their ability to perform post-translational modifications and their successful approval history. The completion of the genome sequence for CHO cells has reignited interest in using quantitative proteomics to identify markers of good production lines. Here we applied two different ...
DOI:: 10.1021/pr501027c
: https://doi.org/10.1021/pr501027c

23. Optimizing production of Fc-amidated peptides by Chinese hamster ovary cells

Author:: Kristina Carlson; Steven C. Pomerantz; Omid Vafa; Michael Naso; William Strohl; Richard E. Mains; Betty A. Eipper
Source:: BMC biotechnology 2015 v.15 no.1 pp. 95
ISSN:: 1472-6750
Subject:: Chinese hamsters; animal ovaries; cell lines; chelation; copper; endoplasmic reticulum; endosomes; enzyme immunoassays; enzymes; glucagon-like peptides; immunoglobulins; lysosomes; mass spectrometry; membrane proteins; molecular weight; peptides
Abstract:: ... BACKGROUND: Amidation of the carboxyl terminal of many peptides is essential for full biological potency, often increasing receptor binding and stability. The single enzyme responsible for this reaction is peptidylglycine α-amidating monooxygenase (PAM: EC 1.14.17.3), a copper- and ascorbate-dependent Type I membrane protein. METHODS: To make large amounts of high molecular weight amidated product ...
DOI:: 10.1186/s12896-015-0210-4
PubMed:: 26475607
PubMed Central:: PMC4609047
: https://doi.org/10.1186/s12896-015-0210-4

24. Transcriptome study and identification of potential marker genes related to the stable expression of recombinant proteins in CHO clones

Author:: Uros Jamnikar; Petra Nikolic; Ales Belic; Marjanca Blas; Dominik Gaser; Andrej Francky; Holger Laux; Andrej Blejec; Spela Baebler; Kristina Gruden
Source:: BMC biotechnology 2015 v.15 no.1 pp. 98
ISSN:: 1472-6750
Subject:: Chinese hamsters; DNA microarrays; algorithms; animal ovaries; bioprocessing; cell lines; chromosome translocation; clones; gene amplification; genes; genetic markers; homologous recombination; karyotyping; methotrexate; post-translational modification; prediction; protein folding; quantitative polymerase chain reaction; recombinant proteins; reverse transcriptase polymerase chain reaction; reverse transcription; transcriptome
Abstract:: ... BACKGROUND: Chinese hamster ovary (CHO) cells have become the host of choice for the production of recombinant proteins, due to their capacity for correct protein folding, assembly, and posttranslational modifications. The most widely used system for recombinant proteins is the gene amplification procedure that uses the CHO-Dhfr expression system. However, CHO cells are known to have a very unstab ...
DOI:: 10.1186/s12896-015-0218-9
PubMed:: 26499110
PubMed Central:: PMC4812793
: https://doi.org/10.1186/s12896-015-0218-9

25. The distribution pattern of α2,3- and α2,6-linked sialic acids affects host cell preference in Toxoplasma gondii

Author:: Minami Baba; Masanao Sato; Katsuya Kitoh; Yasuhiro Takashima
Source:: Experimental parasitology 2015 v.155 pp. 74-81
ISSN:: 0014-4894
Subject:: Chinese hamsters; Toxoplasma gondii; adhesion; animal ovaries; cell lines; confocal laser scanning microscopy; fluorescence; genes; parasites; regression analysis; sialic acid; tachyzoites; transfection; transferases
Abstract:: ... Tachyzoites of Toxoplasma gondii, an obligate intracellular parasite, actively invade almost all types of nucleated cells. However, T. gondii tachyzoites preferentially infect particular types of animal tissue cells. The mechanism underlying the host cell preference of T. gondii is not yet known. In this study, we found that enzymatic removal of α2,3- but not α2,6-linked sialic acids on the surfac ...
DOI:: 10.1016/j.exppara.2015.05.005
: https://doi.org/10.1016/j.exppara.2015.05.005

26. A synthetic peptide inhibits human ovarian cancer cell motility

Author:: Yan Wei; Di Huang; Kaiqun Wang; Jingjing Du; Yinchun Hu
Source:: RSC advances 2015 v.5 no.102 pp. 83801-83808
ISSN:: 2046-2069
Subject:: Western blotting; actin; animal ovaries; antineoplastic activity; cell lines; cell movement; cell viability; cytoskeleton; humans; mitochondria; neoplasm cells; ovarian neoplasms; peptide libraries; phages; phosphatidylinositol 3-kinase; pseudopodia; rough endoplasmic reticulum; signal transduction; staining; synthetic peptides; therapeutics; transmission electron microscopy
Abstract:: ... Phage display libraries have been commonly used to identify recognition sites and antigen–antibody binding systems in tumor cells since the 1990s. In the previous study, we isolated a 12-mer binding peptide SVSVGMKPSPRP (SV-peptide) while isolating human ovarian cancer cells (SK-OV-3) using a random phage display library. Therefore, we tested the specific binding and anticancer properties of the S ...
DOI:: 10.1039/c5ra08755d
: https://doi.org/10.1039/c5ra08755d

27. New halogenated constituents from Mangifera zeylanica Hook.f. and their potential anti-cancer effects in breast and ovarian cancer cells

Author:: Meran Keshawa Ediriweera; Kamani Hemamala Tennekoon; Achyut Adhikari; Sameera Ranganath Samarakoon; Ira Thabrew; E. Dilip de Silva
Source:: Journal of ethnopharmacology 2016 v.189 pp. 165-174
ISSN:: 0378-8741
Subject:: 2,2-diphenyl-1-picrylhydrazyl; Ayurvedic medicine; Mangifera; animal ovaries; antineoplastic activity; antineoplastic agents; apoptosis; bark; breast neoplasms; breasts; caspase-3; catechin; cell cycle; cell lines; chloroform; cytotoxicity; drug therapy; epithelial cells; epithelium; estrogen receptors; gene expression; growth factors; high performance liquid chromatography; histone deacetylase; mammary glands; mass spectrometry; neoplasm cells; nuclear magnetic resonance spectroscopy; ovarian neoplasms; pharmacokinetics; quantitative polymerase chain reaction; quercetin; receptor protein-tyrosine kinase; Sri Lanka
Abstract:: ... Mangifera zeylanica Hook.f. (Anacardiaceae) is a plant endemic to Sri Lanka. Its bark has been used in traditional and Ayurvedic medicine for the treatment of various diseases including some cancers.This study was planned to isolate and identify potentially cytotoxic compounds from the bark of M. zeylanica, which may have contributed to its ethno pharmacological use in the treatment of cancer.The ...
DOI:: 10.1016/j.jep.2016.05.047
: https://doi.org/10.1016/j.jep.2016.05.047

28. Accumulative scFv-Fc antibody gene integration into the hprt chromosomal locus of Chinese hamster ovary cells

Author:: Xue Wang; Yoshinori Kawabe; Risa Kato; Takeshi Hada; Akira Ito; Yoshimasa Yamana; Masako Kondo; Masamichi Kamihira
Source:: Journal of bioscience and bioengineering 2017 v.124 no.5 pp. 583-590
ISSN:: 1389-1723
Subject:: Chinese hamsters; animal ovaries; antibodies; biopharmaceuticals; cell lines; color; enzymes; fluorescent proteins; loci; models; protein synthesis; transfection; transgenes
Abstract:: ... We have previously developed an accumulative site-specific gene integration system (AGIS) using Cre-recombinase and mutated loxP sites. AGIS enables repeated transgene integration into a predetermined chromosomal site in mammalian cells. However, the process of establishing cells with multiple integrated copies of the transgene is still time-consuming. In the present study, we describe an improved ...
DOI:: 10.1016/j.jbiosc.2017.05.017
: https://doi.org/10.1016/j.jbiosc.2017.05.017

29. CHO cell cytotoxicity and genotoxicity analyses of disinfection by-products: An updated review

Author:: Elizabeth D. Wagner; Michael J. Plewa
Source:: Journal of environmental sciences (China) 2017 v.58 pp. 64-76
ISSN:: 1001-0742
Subject:: Chinese hamsters; adverse effects; analytical chemistry; animal ovaries; bromides; byproducts; cell lines; cytotoxicity; disinfectants; disinfection; epidemiological studies; genotoxicity; health services; iodides; mutagens; neurotoxicity; organic matter; public health; tap water; teratogenicity; toxicology
Abstract:: ... The disinfection of drinking water is an important public health service that generates high quality, safe and palatable tap water. The disinfection of drinking water to reduce waterborne disease was an outstanding public health achievement of the 20th century. An unintended consequence is the reaction of disinfectants with natural organic matter, anthropogenic contaminants and bromide/iodide to f ...
DOI:: 10.1016/j.jes.2017.04.021
: https://doi.org/10.1016/j.jes.2017.04.021

30. Evaluating the expression profile and stability of different UCOE containing vector combinations in mAb-producing CHO cells

Author:: Fatemeh Nematpour; Fereidoun Mahboudi; Behrouz Vaziri; Vahid Khalaj; Samira Ahmadi; Maryam Ahmadi; Saedeh Ebadat; Fatemeh Davami
Source:: BMC biotechnology 2017 v.17 no.1 pp. 18
ISSN:: 1472-6750
Subject:: Chinese hamsters; animal ovaries; cell lines; chromatin; gene silencing; genes; manufacturing; monoclonal antibodies; transcription (genetics)
Abstract:: ... BACKGROUND: As the demand for monoclonal antibodies (mAb) increases, more efficient expression methods are required for their manufacturing process. Transcriptional gene silencing is a common phenomenon in recombinant cell lines which leads to expression reduction and instability. There are reports on improved antibody expression in ubiquitous chromatin opening element (UCOE) containing both heavy ...
DOI:: 10.1186/s12896-017-0330-0
PubMed:: 28228095
PubMed Central:: PMC5322649
: https://doi.org/10.1186/s12896-017-0330-0

31. Lessons from the Hamster: Cricetulus griseus Tissue and CHO Cell Line Proteome Comparison

Author:: Heffner Kelley M.; Hizal Deniz Baycin; Yerganian George S.; Kumar Amit; Can Özge; O’Meally Robert; Cole Robert; Chaerkady Raghothama; Wu Herren; Bowen Michael A.; Betenbaugh Michael J.
Source:: Journal of Proteome Research 2017 v.16 no.10 pp. 3672-3687
ISSN:: 1535-3907
Subject:: Chinese hamsters; Golgi apparatus; animal ovaries; biopharmaceuticals; cell cycle; cell lines; data collection; developmental stages; gene ontology; genetic variation; glycosylation; lipids; liver; peptides; proteome; proteomics; recombinant proteins; tissues
Abstract:: ... Chinese hamster ovary cells represent the dominant host for therapeutic recombinant protein production. However, few large-scale data sets have been generated to characterize this host organism and derived CHO cell lines at the proteomics level. Consequently, an extensive label-free quantitative proteomics analysis of two cell lines (CHO-S and CHO DG44) and two Chinese hamster tissues (liver and o ...
DOI:: 10.1021/acs.jproteome.7b00382
: https://doi.org/10.1021/acs.jproteome.7b00382

32. LongR3 enhances Fc-fusion protein N-linked glycosylation while improving protein productivity in an industrial CHO cell line

Author:: Yueming Qian; Amanda M. Lewis; Sarah M. Sidnam; Alison Bergeron; Nicholas R. Abu-Absi; Nisha Vaidyanathan; Adam Deresienski; Nan-Xin Qian; Michael C. Borys; Zheng Jian Li
Source:: Process biochemistry 2017 v.53 pp. 201-209
ISSN:: 1359-5113
Subject:: Chinese hamsters; animal ovaries; cell culture; cell lines; cost effectiveness; enzyme activity; gene expression regulation; genes; glycoproteins; glycosylation; industry; insulin-like growth factor I; polymerase chain reaction; polysaccharides; recombinant proteins; reverse transcription; sialic acid; sialidase; therapeutics
Abstract:: ... Glycosylation is a critical quality attribute for many therapeutic glycoproteins. Numerous studies in academia and industry have sought to understand and control protein glycosylation in order to obtain consistent glycoform profiles in production. In many cases, the resulting enhancement of protein glycosylation comes at the expense of protein productivity. Here we examine the impact of insulin-li ...
DOI:: 10.1016/j.procbio.2016.11.018
: https://doi.org/10.1016/j.procbio.2016.11.018

33. Accelerated Barocycler Lysis and Extraction Sample Preparation for Clinical Proteomics by Mass Spectrometry

Author:: Natasha Lucas; Andrew B. Robinson; Maiken Marcker Espersen; Sadia Mahboob; Dylan Xavier; Jing Xue; Rosemary L. Balleine; Anna deFazio; Peter G. Hains; Phillip J. Robinson
Source:: Journal of proteome research 2018 v.18 no.1 pp. 399-405
ISSN:: 1535-3907
Subject:: 1-propanol; animal ovaries; animal tissues; biopsy; brain; cell lines; deoxycholic acid; humans; kidneys; lungs; mass spectrometry; muscles; ovarian neoplasms; proteins; proteolysis; proteome; proteomics; rats; solubilization; testes; urea
Abstract:: ... We have developed a streamlined proteomic sample preparation protocol termed Accelerated Barocycler Lysis and Extraction (ABLE) that substantially reduces the time and cost of tissue sample processing. ABLE is based on pressure cycling technology (PCT) for rapid tissue solubilization and reliable, controlled proteolytic digestion. Here, a previously reported PCT based protocol was optimized using ...
DOI:: 10.1021/acs.jproteome.8b00684
: https://doi.org/10.1021/acs.jproteome.8b00684

34. All-trans retinoic acid in combination with sodium butyrate enhances specific monoclonal antibody productivity in recombinant CHO cell line

Author:: Mahmood Rahimi-Zarchi; Seyed Abbas Shojaosadati; Mohammad Mehdi Amiri; Mahmood Jeddi-Tehrani; Fazel Shokri
Source:: Bioprocess and biosystems engineering 2018 v.41 no.7 pp. 961-971
ISSN:: 1615-7591
Subject:: Chinese hamsters; animal ovaries; antibody formation; binding capacity; cell culture; cell lines; developmental stages; models; monoclonal antibodies; retinoic acid; sodium butyrate; temperature; viability
Abstract:: ... The effects of all-trans retinoic acid (RA) and sodium butyrate (NaBu) on growth, viability and antibody production of two types of transfected Chinese hamster ovary cell lines (CHO-K1 and CHO-S) were investigated using a batch mode cell culture. By adding 0.5 mM NaBu in the CHO-K1 cell culture, the cell specific productivity (Qₚ) and antibody concentration increased by five- and threefold, respec ...
DOI:: 10.1007/s00449-018-1927-y
: https://doi.org/10.1007/s00449-018-1927-y

35. Amelioration of arsenic-induced oxidative stress in CHO cells by Ixora coccinea flower extract

Author:: S. A. Salmataj; Shobha U. Kamath; V. Ramachandra Murty; Sreedhara Ranganath Pai
Source:: 3 Biotech 2018 v.8 no.10 pp. 446
ISSN:: 2190-572X
Subject:: 2,2-diphenyl-1-picrylhydrazyl; Chinese hamsters; DNA damage; Ixora coccinea; animal ovaries; antioxidant activity; apoptosis; arsenic; catalase; cell lines; chronic exposure; cytotoxicity; flower extracts; flowers; free radical scavengers; genotoxicity; inhibitory concentration 50; mitochondrial membrane; nitric oxide; oxidative stress; sodium arsenate; superoxide dismutase
Abstract:: ... Chronic exposure to inorganic arsenic creates various health problems. Ixora coccinea flower extract was investigated for its ability to protect against arsenic-induced cytotoxicity and genotoxicity in CHO cell line. MTT assay confirmed the efficacy of the extract in ameliorating arsenic-induced cytotoxicity. The value (48 mM) of 24 h inhibitory concentration (IC50) of sodium arsenate for CHO cell ...
DOI:: 10.1007/s13205-018-1446-1
PubMed:: 30333948
PubMed Central:: PMC6177355
: https://doi.org/10.1007/s13205-018-1446-1

36. Applications of proteomic methods for CHO host cell protein characterization in biopharmaceutical manufacturing

Author:: Kristin N Valente; Nicholas E Levy; Kelvin H Lee; Abraham M Lenhoff
Source:: Current opinion in biotechnology 2018 v.53 pp. 144-150
ISSN:: 0958-1669
Subject:: Chinese hamsters; additives; animal ovaries; biopharmaceuticals; cell lines; chromatography; enzyme-linked immunosorbent assay; genetic engineering; manufacturing; monoclonal antibodies; product quality; proteomics; temperature; viability
Abstract:: ... Chinese hamster ovary (CHO) cells are the most prevalent host organism for production of recombinant therapeutic proteins, including monoclonal antibodies (mAbs). Regulatory guidance mandates control of the host cell protein (HCP) concentration in the production process, which remains a primary challenge. Although HCP concentrations are typically measured by ELISA, orthogonal proteomic methods are ...
DOI:: 10.1016/j.copbio.2018.01.004
: https://doi.org/10.1016/j.copbio.2018.01.004

37. A CRISPR/Cas9 based engineering strategy for overexpression of multiple genes in Chinese hamster ovary cells

Author:: Peter Eisenhut; Gerald Klanert; Marcus Weinguny; Laurenz Baier; Vaibhav Jadhav; Daniel Ivansson; Nicole Borth
Source:: Metabolic engineering 2018 v.48 pp. 72-81
ISSN:: 1096-7176
Subject:: Chinese hamsters; RNA; animal ovaries; biopharmaceuticals; cell lines; engineering; gene overexpression; genes; phenotype; plasmids; protein synthesis; screening; transcription termination; transfection
Abstract:: ... Manipulation of multiple genes to engineer Chinese Hamster Ovary (CHO) cells for better performance in production processes of biopharmaceuticals has recently become more and more popular. Yet, identification of useful genes and the unequivocally assessment of their effect alone and in combination(s) on the cellular phenotype is difficult due to high variation between subclones. Here, we present d ...
DOI:: 10.1016/j.ymben.2018.05.017
: https://doi.org/10.1016/j.ymben.2018.05.017

38. Clonal variation in productivity and proteolytic clipping of an Fc-fusion protein in CHO cells: Proteomic analysis suggests a role for defective protein folding and the UPR

Author:: Michael Henry; Clair Gallagher; Ronan M. Kelly; Christopher C. Frye; Matthew D. Osborne; Ciaran P. Brady; Niall Barron; Martin Clynes; Paula Meleady
Source:: Journal of biotechnology 2018 v.281 pp. 21-30
ISSN:: 0168-1656
Subject:: Chinese hamsters; animal ovaries; biopharmaceutical industry; cell lines; clonal variation; clones; development projects; gene ontology; phenotype; protein folding; protein synthesis; proteinases; proteins; proteolysis; proteomics; translation (genetics)
Abstract:: ... Product degradation, such as clipping, is a common quality issue in the production of Fc-fusion proteins from Chinese hamster ovary (CHO) cells. Degradation of proteins is mainly due to the action of either intracellular or extracellular host cell proteases. This study was carried out to understand more fundamentally the intracellular events that may play a role in determining why cell lines from ...
DOI:: 10.1016/j.jbiotec.2018.05.018
: https://doi.org/10.1016/j.jbiotec.2018.05.018

39. Comprehensive Glycoproteomic Analysis of Chinese Hamster Ovary Cells

Author:: Ganglong Yang; Yingwei Hu; Shisheng Sun; Chuanzi Ouyang; Weiming Yang; Qiong Wang; Michael Betenbaugh; Hui Zhang
Source:: Analytical chemistry 2018 v.90 no.24 pp. 14294-14302
ISSN:: 1520-6882
Subject:: Chinese hamsters; adverse effects; animal ovaries; antibodies; biopharmaceuticals; cell lines; erythropoietin; fractionation; glycomics; glycopeptides; glycoproteomics; glycosylation; hydrophilicity; reversed-phase liquid chromatography; sequons; tandem mass spectrometry; therapeutics
Abstract:: ... The Chinese hamster ovary (CHO) cell line is a major expression system for the production of therapeutic proteins, the majority of which are glycoproteins, such as antibodies and erythropoietin (EPO). The characterization glycosylation profile of therapeutic proteins produced from engineered CHO cells and therapeutic functions, as well as side effects, are critical to understand the important role ...
DOI:: 10.1021/acs.analchem.8b03520
PubMed:: 30457839
PubMed Central:: PMC6440468
: https://doi.org/10.1021/acs.analchem.8b03520

40. Debottlenecking protein secretion and reducing protein aggregation in the cellular host

Author:: Yizhou Zhou; Ravali Raju; Christina Alves; Alan Gilbert
Source:: Current opinion in biotechnology 2018 v.53 pp. 151-157
ISSN:: 0958-1669
Subject:: Chinese hamsters; animal ovaries; biopharmaceuticals; cell lines; protein secretion; protein synthesis; proteins; technology; therapeutics
Abstract:: ... Chinese hamster ovary (CHO) cells have been extensively used for industrial production of biotherapeutics. With advances in cell line development and process optimization, production levels of therapeutic proteins using the CHO expression system have increased to beyond 10g per liter scale. These high-titer processes could challenge the secretory capacity of CHO cells, which can result in degradat ...
DOI:: 10.1016/j.copbio.2018.01.007
: https://doi.org/10.1016/j.copbio.2018.01.007

41. Development of hyper osmotic resistant CHO host cells for enhanced antibody production

Author:: Yasuharu Kamachi; Takeshi Omasa
Source:: Journal of bioscience and bioengineering 2018 v.125 no.4 pp. 470-478
ISSN:: 1389-1723
Subject:: Chinese hamsters; animal ovaries; antibody formation; cell culture; cell growth; cell lines; cell viability; culture media; feeding methods; monoclonal antibodies; nutrients; osmolality; osmotic stress; product development
Abstract:: ... Cell culture platform processes are generally employed to shorten the duration of new product development. A fed-batch process with continuous feeding is a conventional platform process for monoclonal antibody production using Chinese hamster ovary (CHO) cells. To establish a simplified platform process, the feeding method can be changed from continuous feed to bolus feed. However, this change ind ...
DOI:: 10.1016/j.jbiosc.2017.11.002
: https://doi.org/10.1016/j.jbiosc.2017.11.002

42. Ecotoxicological potential of the biocides terbutryn, octhilinone and methylisothiazolinone: Underestimated risk from biocidal pathways?

Author:: Simon Kresmann; Arokia Hansel Rajan Arokia; Christoph Koch; Bernd Sures
Source:: Science of the total environment 2018 v.625 pp. 900-908
ISSN:: 0048-9697
Subject:: Chinese hamsters; Daphnia magna; Oncorhynchus mykiss; animal ovaries; aquatic environment; biocides; cell lines; cleaning agents; coatings; databases; ecotoxicology; environmental assessment; household products; households; industry; leaching; liver; median effective concentration; models; pollution; risk; risk assessment; terbutryn
Abstract:: ... The use of biocides by industry, agriculture and households increased throughout the last two decades. Many new applications with known substances enriched the variety of biocidal pollution sources for the aquatic environment. While agriculture was the major source for a long time, leaching from building facades and preservation of personal care and cleaning products was identified as new sources ...
DOI:: 10.1016/j.scitotenv.2017.12.280
: https://doi.org/10.1016/j.scitotenv.2017.12.280

43. Engineering a stable CHO cell line for the expression of a MERS-coronavirus vaccine antigen

Author:: Mun Peak Nyon; Lanying Du; Chien-Te Kent Tseng; Christopher A. Seid; Jeroen Pollet; Kevin S. Naceanceno; Anurodh Agrawal; Abdullah Algaissi; Bi-Hung Peng; Wanbo Tai; Shibo Jiang; Maria Elena Bottazzi; Ulrich Strych; Peter J. Hotez
Source:: Vaccine 2018 v.36 no.14 pp. 1853-1862
ISSN:: 0264-410X
Subject:: Chinese hamsters; Middle East respiratory syndrome coronavirus; adjuvants; animal ovaries; antigens; cell lines; humans; immunoglobulin G; interleukin-2; manufacturing; methotrexate; mice; neutralizing antibodies; patients; plasmids; recombinant proteins; secretion; therapeutics; toxicity; transgenic animals; vaccines
Abstract:: ... Middle East respiratory syndrome coronavirus (MERS-CoV) has infected at least 2040 patients and caused 712 deaths since its first appearance in 2012, yet neither pathogen-specific therapeutics nor approved vaccines are available. To address this need, we are developing a subunit recombinant protein vaccine comprising residues 377–588 of the MERS-CoV spike protein receptor-binding domain (RBD), whi ...
DOI:: 10.1016/j.vaccine.2018.02.065
PubMed:: 29496347
PubMed Central:: PMC5860679
: https://doi.org/10.1016/j.vaccine.2018.02.065

44. Enhanced production of anti-PD1 antibody in CHO cells through transient co-transfection with anti-apoptotic genes Bcl-x L and Mcl-1

Author:: Xinyu Zhang; Lei Han; Huifang Zong; Kai Ding; Yuan Yuan; Jingyi Bai; Yuexian Zhou; Baohong Zhang; Jianwei Zhu
Source:: Bioprocess and biosystems engineering 2018 v.41 no.5 pp. 633-640
ISSN:: 1615-7591
Subject:: Chinese hamsters; animal ovaries; antibodies; apoptosis; biopharmaceuticals; cell culture; cell lines; cell viability; cost effectiveness; gene overexpression; genes; polyethyleneimine; screening
Abstract:: ... Apoptosis has a negative impact on the cell survival state during cell cultivation. To optimize mammalian cell culture for production of biopharmaceuticals, one of the important approaches is to extend cell life through over-expression of anti-apoptotic genes. Here, we reported a cost-effective process to enhance cell survival and production of an antibody through transient co-transfection with an ...
DOI:: 10.1007/s00449-018-1898-z
: https://doi.org/10.1007/s00449-018-1898-z

45. Enhancement of glycosylation by stable co-expression of two sialylation-related enzymes on Chinese hamster ovary cells

Author:: Nguyen Thi Sam; Ryo Misaki; Takao Ohashi; Kazuhito Fujiyama
Source:: Journal of bioscience and bioengineering 2018 v.126 no.1 pp. 102-110
ISSN:: 1389-1723
Subject:: Chinese hamsters; animal ovaries; bioactive properties; biopharmaceuticals; cell lines; glycoproteins; glycosidases; glycosylation; glycosyltransferases; half life; humans; ions; proteinases; sialic acid
Abstract:: ... Sialic acid plays important roles in stabilization and modulation of the interaction of molecules and membranes in organisms. Due to its high electronegativity, sialic acid can promote binding effects of molecules and support the transportation of drugs and ions in cells. This also strengthens cells against degradation from glycosidases and proteases. Hence sialic acid helps glycoproteins extend t ...
DOI:: 10.1016/j.jbiosc.2018.01.010
: https://doi.org/10.1016/j.jbiosc.2018.01.010

46. Evaluation of fluorescent dyes to measure protein aggregation within mammalian cell culture supernatants

Author:: Sheun Oshinbolu; Rachana Shah; Gary Finka; Mike Molloy; Mark Uden; Daniel G Bracewell
Source:: Journal of chemical technology and biotechnology 2018 v.93 no.3 pp. 909-917
ISSN:: 0268-2575
Subject:: Chinese hamsters; animal ovaries; bioprocessing; cell culture; cell lines; cell viability; fluorescence; fluorescent dyes; molecular weight; monoclonal antibodies; protein aggregates
Abstract:: ... BACKGROUND: A current challenge in bioprocessing is the ability to analyse critical quality attributes such as aggregation without prior purification. This study evaluated the use of fluorescent dyes (Bis‐ANS, SYPRO Orange, Thioflavin T and ProteoStat) to characterise mAb aggregates in Chinese hamster ovary clarified cultures. RESULTS: The null and mAb culture supernatants showed an increase in fl ...
DOI:: 10.1002/jctb.5519
PubMed:: 29540956
PubMed Central:: PMC5838538
: https://doi.org/10.1002/jctb.5519

47. Glyco-Mapper: A Chinese hamster ovary (CHO) genome-specific glycosylation prediction tool

Author:: Benjamin G. Kremkow; Kelvin H. Lee
Source:: Metabolic engineering 2018 v.47 pp. 134-142
ISSN:: 1096-7176
Subject:: Chinese hamsters; animal ovaries; cell engineering; cell lines; feed composition; feeding methods; genes; glycosylation; models; polysaccharides; prediction; product quality; sugars
Abstract:: ... Glyco-Mapper is a novel systems biology product quality prediction tool created using a new framework termed: Discretized Reaction Network Modeling using Fuzzy Parameters (DReaM-zyP). Within Glyco-Mapper, users fix the nutrient feed composition and the glycosylation reaction fluxes to fit the model glycoform to the reference experimental glycoform, enabling cell-line specific glycoform predictions ...
DOI:: 10.1016/j.ymben.2018.03.002
: https://doi.org/10.1016/j.ymben.2018.03.002

48. In situ base release for pH maintenance can allow shake flasks to better mimic bioreactor performance for CHO cell culture

Author:: Vishwanathgouda Maralingannavar; Bharath R Shenoy; Jahnabi Hazarika; Divya Unnikrishnan; Anuja Prabhu; Sunit Maity; Mugdha Gadgil
Source:: Journal of chemical technology and biotechnology 2018 v.93 no.10 pp. 2842-2850
ISSN:: 0268-2575
Subject:: Chinese hamsters; animal ovaries; bioreactors; cell culture; cell growth; cell lines; clones; culture flasks; glycosylation; hydrogels; immunoglobulin G; infrastructure; lactic acid; pH; screening
Abstract:: ... BACKGROUND: Shake flasks are widely used for evaluating mammalian cells in suspension. Lack of pH control can contribute to differences in culture performance between them and bioreactors. This study evaluates whether a previously reported in situ base releasing hydrogel (pHmH) to counter pH decrease can enable shake flask cultures to better mimic bioreactor cultures. RESULTS: Compared with biorea ...
DOI:: 10.1002/jctb.5635
: https://doi.org/10.1002/jctb.5635

49. Inactivation of deubiquitinase CYLD enhances therapeutic antibody production in Chinese hamster ovary cells

Author:: Yafang Lu; Qin Zhou; Qianqian Han; Pengfei Wu; Lanlan Zhang; Lin Zhu; David T. Weaver; Changzhi Xu; Buchang Zhang
Source:: Applied microbiology and biotechnology 2018 v.102 no.14 pp. 6081-6093
ISSN:: 0175-7598
Subject:: Chinese hamsters; animal ovaries; antibodies; antibody formation; apoptosis; cell engineering; cell lines; cell proliferation; chromosomes; genetic engineering; immunoglobulin G; manufacturing; mutation; neoplasms; plasmids; signal transduction; therapeutics; transcription factor NF-kappa B
Abstract:: ... Chinese hamster ovary (CHO) cells are promising host engineering cells for industry manufacturing of therapeutic antibodies. However, cell death due to apoptosis remains a huge challenge to augment antibody production, and developing CHO cells with enhanced anti-apoptosis and proliferation ability is fundamental for cell line development and high-yielding bioprocesses. Deubiquitinase cylindromatos ...
DOI:: 10.1007/s00253-018-9070-x
: https://doi.org/10.1007/s00253-018-9070-x

50. Integrated use of bioinformatic resources reveals that co-targeting of histone deacetylases, IKBK and SRC inhibits epithelial-mesenchymal transition in cancer

Author:: Farnaz Barneh; Mehdi Mirzaie; Payman Nickchi; Tuan Zea Tan; Jean Paul Thiery; Mehran Piran; Mona Salimi; Fatemeh Goshadrou; Amir R Aref; Mohieddin Jafari
Source:: Briefings in bioinformatics 2018 v.20 no.2 pp. 717-731
ISSN:: 1477-4054
Subject:: animal ovaries; bioinformatics; case studies; cell lines; clinical trials; coculture; collagen; computer simulation; drugs; endothelial cells; epithelium; genes; histone deacetylase; lung neoplasms; neoplasm cells; ovarian neoplasms; patients; phosphotransferases (kinases); prognosis; signal transduction; statistical analysis; therapeutics
Abstract:: ... With the advent of high-throughput technologies leading to big data generation, increasing number of gene signatures are being published to predict various features of diseases such as prognosis and patient survival. However, to use these signatures for identifying therapeutic targets, use of additional bioinformatic tools is indispensible part of research. Here, we have generated a pipeline compr ...
DOI:: 10.1093/bib/bby030
: https://doi.org/10.1093/bib/bby030

51. Intracellular response of CHO cells to oxidative stress and its influence on metabolism and antibody production

Author:: Michael W. Handlogten; Min Zhu; Sanjeev Ahuja
Source:: Biochemical engineering journal 2018 v.133 pp. 12-20
ISSN:: 1369-703X
Subject:: Chinese hamsters; air; amino acids; animal ovaries; antibody formation; biochemical pathways; bioreactors; cell lines; dissolved oxygen; enzyme activity; glutathione; homeostasis; hydrogen peroxide; manufacturing; metabolism; mitochondria; oxidative stress; oxygen; viability
Abstract:: ... Chinese hamster ovary (CHO) cells are widely used for the manufacture of therapeutic mAbs with high productivity processes generating 5–10 g/L mAb. High productivity processes impose considerable strain on the intracellular metabolic and redox homeostasis resulting in increased oxidative stress. We investigated the mechanisms by which oxidative stress affects the productivity of a mAb producing CH ...
DOI:: 10.1016/j.bej.2018.01.031
: https://doi.org/10.1016/j.bej.2018.01.031

52. LncRNA ADAMTS9-AS2 regulates ovarian cancer progression by targeting miR-182-5p/FOXF2 signaling pathway

Author:: Aihong Wang; Canhui Jin; Hongyu Li; Qiaohong Qin; Lei Li
Source:: International journal of biological macromolecules 2018 v.120 pp. 1705-1713
ISSN:: 0141-8130
Subject:: animal ovaries; cell lines; cell proliferation; lymph nodes; metastasis; non-coding RNA; ovarian neoplasms; patients; signal transduction; therapeutics
Abstract:: ... Increasing studies revealed that aberrant expression of long non-coding RNAs (lncRNAs) play critical roles in ovarian cancer (OC) progression. However, the roles and underlying mechanisms of ADAMTS9-AS2 in OC remain unclear. In the present study, we showed that ADAMTS9-AS2 expression was significantly decreased in OC tissues and cell lines. Low ADAMTS9-AS2 expression was correlated with advanced F ...
DOI:: 10.1016/j.ijbiomac.2018.09.179
: https://doi.org/10.1016/j.ijbiomac.2018.09.179

53. LncRNA LINC00319 accelerates ovarian cancer progression through miR-423-5p/NACC1 pathway

Author:: Wenling Du; Zejiao Feng; Qinqin Sun
Source:: Biochemical and biophysical research communications 2018 v.507 no.1-4 pp. 198-202
ISSN:: 0006-291X
Subject:: Western blotting; animal ovaries; bioinformatics; biomarkers; carcinogenesis; cell lines; disease course; luciferase; neoplasm cells; non-coding RNA; ovarian neoplasms; patients; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; squamous cell carcinoma; tissues
Abstract:: ... Long noncoding RNA (lncRNA) LINC00319 has been reported to promote carcinogenesis of lung cancer and cutaneous squamous cell carcinoma. However, the role and mechanism of LINC00319 in ovarian cancer progression is unclear. In this study, LINC00319 expression was found to be upregulated in ovarian cancer tissues and cell lines. And our evidence showed that LINC00319 could be a potential prognostic ...
DOI:: 10.1016/j.bbrc.2018.11.006
: https://doi.org/10.1016/j.bbrc.2018.11.006

54. Multiple reaction monitoring targeted LC-MS analysis of potential cell death marker proteins for increased bioprocess control

Author:: Simone Albrecht; Christian Kaisermayer; David Reinhart; Monica Ambrose; Renate Kunert; Anna Lindeberg; Jonathan Bones
Source:: Analytical and bioanalytical chemistry 2018 v.410 no.13 pp. 3197-3207
ISSN:: 1618-2642
Subject:: Chinese hamsters; animal ovaries; biomarkers; bioprocessing; bioreactors; cell death; cell growth; cell lines; death; growth models; liquid chromatography; mass spectrometry; monitoring; monoclonal antibodies; prediction; proteins; proteomics; viability
Abstract:: ... The monitoring of protein biomarkers for the early prediction of cell stress and death is a valuable tool for process characterization and efficient biomanufacturing control. A representative set of six proteins, namely GPDH, PRDX1, LGALS1, CFL1, TAGLN2 and MDH, which were identified in a previous CHO-K1 cell death model using discovery LC-MSᴱ was translated into a targeted liquid chromatography m ...
DOI:: 10.1007/s00216-018-1029-3
: https://doi.org/10.1007/s00216-018-1029-3

55. PTP1B promotes the malignancy of ovarian cancer cells in a JNK-dependent mechanism

Author:: Wenyan Wang; Yunxia Cao; Xiao Zhou; Bing Wei; Yu Zhang; Xiaochun Liu
Source:: Biochemical and biophysical research communications 2018 v.503 no.2 pp. 903-909
ISSN:: 0006-291X
Subject:: animal ovaries; carcinogenesis; cell lines; death; epigenetics; metastasis; mice; mitogen-activated protein kinase; neoplasm cells; ovarian neoplasms; phenotype; signal transduction; women
Abstract:: ... Ovarian cancer is the leading cause of death from gynecological malignancies in women. Diagnosis at the early stage remains challenging and efficient treatment is still highly needed. The development and progression of this cancer is associated with many genetic and epigenetic changes, representing the dysregulation of a highly complex signaling network. Previous studies found that protein-tyrosin ...
DOI:: 10.1016/j.bbrc.2018.06.094
: https://doi.org/10.1016/j.bbrc.2018.06.094

56. Pathological analysis of silkworm infected by two microsporidia Nosema bombycis CQ1 and Vairimorpha necatrix BM

Author:: Xian-Zhi Meng; Bo Luo; Xiang-You Tang; Qiang He; Ting-Rong Xiong; Zhuo-Ya Fang; Guoqing Pan; Tian Li; Ze-Yang Zhou
Source:: Journal of invertebrate pathology 2018 v.153 pp. 75-84
ISSN:: 0022-2011
Subject:: Bombyx mori; Nosema bombycis; Vairimorpha necatrix; animal ovaries; cell lines; connective tissues; epithelial cells; intestines; pathogens; scanning electron microscopy; silkworms; spores; testes; transmission electron microscopy; virulence
Abstract:: ... Microsporidia Nosema bombycis CQ1 can be vertically transmitted in silkworm Bombyx mori but Vairimorpha necatrix BM cannot. Therefore, the pathological differences in silkworm infected with these two microsporidia required clarification. Here, we compared the virulence of N. bombycis CQ1 and V. necatrix BM against silkworm. The pathological characteristics in intestine, testis and ovary were surve ...
DOI:: 10.1016/j.jip.2017.12.005
: https://doi.org/10.1016/j.jip.2017.12.005

57. Perspectives on progressive strategies and recent trends in the production of recombinant human factor VIII

Author:: S.T. Mannully; Ramya L.N.; K.K. Pulicherla
Source:: International journal of biological macromolecules 2018 v.119 pp. 496-504
ISSN:: 0141-8130
Subject:: Chinese hamsters; animal ovaries; bioprocessing; blood; blood coagulation; cell lines; factor VIII; freeze drying; glycoproteins; glycosylation; hemophilia; humans; patients; product safety; therapeutics
Abstract:: ... Factor VIII (fVIII), a glycoprotein cofactor, plays a crucial role in the intrinsic blood coagulation pathway. As one of the most essential blood clotting factors known today, fVIII is the largest and most complex commercialized therapeutic protein used in the treatment of hemophilia A, an X-linked recessive disorder. Two lyophilized fVIII concentrates (viz., plasma fractionated and recombinant) a ...
DOI:: 10.1016/j.ijbiomac.2018.07.164
: https://doi.org/10.1016/j.ijbiomac.2018.07.164

58. Physiological responses of Chinese hamster ovary cells to a productivity-enhancing yeast extract

Author:: Dongdong Hu; Liang Zhao; Jiaqi Wang; Li Fan; Xuping Liu; Haibin Wang; Wen-Song Tan
Source:: Journal of bioscience and bioengineering 2018 v.126 no.5 pp. 636-643
ISSN:: 1389-1723
Subject:: Chinese hamsters; animal ovaries; cell culture; cell engineering; cell lines; energy; energy metabolism; fatty acids; hydrolysates; metabolites; nucleic acids; nucleotides; physiological response; protein synthesis; recombinant proteins; yeast extract
Abstract:: ... Hydrolysates play important roles in enhancing the productivity of recombinant proteins in mammalian cell cultures. Lacking of detailed understanding of the mechanisms, hydrolysate is commonly regarded as an unstable factor which should be used with cautions. A yeast extract (YE) was approved to improve the Fc-fusion protein productivity in a recombinant Chinese hamster ovary (CHO) cell line. To e ...
DOI:: 10.1016/j.jbiosc.2018.05.005
: https://doi.org/10.1016/j.jbiosc.2018.05.005

59. Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma

Author:: Shing-Jyh Chang; En-Chi Liao; Hsin-Yueh Yeo; Wen-Hung Kuo; Hsin-Yi Chen; Yi-Ting Tsai; Yu-Shan Wei; Ying-Jen Chen; Yi-Shiuan Wang; Ji-Min Li; Chuan-Chi Shih; Chia-Hao Chan; Zih-Yin Lai; Hsiu-Chuan Chou; Yung-Jen Chuang; Hong-Lin Chan
Source:: Archives of biochemistry and biophysics 2018 v.647 pp. 10-32
ISSN:: 0003-9861
Subject:: DNA damage; DNA repair; animal ovaries; apoptosis; biosynthesis; cell cycle checkpoints; cell lines; cell proliferation; humans; nucleoside-diphosphate kinase; ovarian neoplasms; precision medicine; proteomics; tumor suppressor proteins
Abstract:: ... With the concept of precision medicine, combining multiple molecular-targeting therapies has brought new approaches to current cancer treatments. Malfunction of the tumor suppressor protein, p53 is a universal hallmark in human cancers. Under normal conditions, p53 is degraded through an ubiquitin-proteosome pathway regulated by its negative regulator, MDM2. In contrast, cellular stress such as DN ...
DOI:: 10.1016/j.abb.2018.04.004
: https://doi.org/10.1016/j.abb.2018.04.004

60. Rapid development of stable transgene CHO cell lines by CRISPR/Cas9-mediated site-specific integration into C12orf35

Author:: Menglin Zhao; Jiaxian Wang; Manyu Luo; Han Luo; Meiqi Zhao; Lei Han; Mengxiao Zhang; Hui Yang; Yueqing Xie; Hua Jiang; Lei Feng; Huili Lu; Jianwei Zhu
Source:: Applied microbiology and biotechnology 2018 v.102 no.14 pp. 6105-6117
ISSN:: 0175-7598
Subject:: Chinese hamsters; animal ovaries; binding capacity; cell lines; genetic engineering; high-throughput nucleotide sequencing; loci; monoclonal antibodies; mutation; protein synthesis; recombinant proteins; transcription (genetics); transgenes
Abstract:: ... Chinese hamster ovary (CHO) cells are the most widely used mammalian hosts for recombinant protein production. However, by conventional random integration strategy, development of a high-expressing and stable recombinant CHO cell line has always been a difficult task due to the heterogenic insertion and its caused requirement of multiple rounds of selection. Site-specific integration of transgenes ...
DOI:: 10.1007/s00253-018-9021-6
: https://doi.org/10.1007/s00253-018-9021-6

61. Strategy for Generating Sequence-Defined Aptamer Reagent Sets for Detecting Protein Contaminants in Biotherapeutics

Author:: James B. McGivney; Andrew T. Csordas; Faye M. Walker; Elizabeth R. Bagley; Emily M. Gruber; Peter L. Mage; Jose Casas-Finet; Margaret A. Nakamoto; Michael Eisenstein; Christopher J. Larkin; Robert J. Strouse; H. Tom Soh
Source:: Analytical chemistry 2018 v.90 no.5 pp. 3262-3269
ISSN:: 1520-6882
Subject:: Chinese hamsters; animal ovaries; cell lines; drugs; isoelectric point; nucleotide aptamers; patients; polyclonal antibodies; proteins; screening; therapeutics; toxicity
Abstract:: ... Biologic drugs are typically manufactured in mammalian host cells, and it is critical from a drug safety and efficacy perspective to detect and remove host cell proteins (HCPs) during production. This is currently achieved with sets of polyclonal antibodies (pAbs), but these suffer from critical shortcomings because their composition is inherently undefined, and they cannot detect nonimmunogenic H ...
DOI:: 10.1021/acs.analchem.7b04775
: https://doi.org/10.1021/acs.analchem.7b04775

62. Transition-metal-free C–N and C–C formation: synthesis of benzo[4,5]imidazo[1,2-a]pyridines and 2-pyridones from ynones

Author:: Qing-Hu Teng; Xiang-Jun Peng; Zu-Yu Mo; Yan-Li Xu; Hai-Tao Tang; Heng-Shan Wang; Hong-Bin Sun; Ying-Ming Pan
Source:: Green chemistry 2018 v.20 no.9 pp. 2007-2012
ISSN:: 1463-9270
Subject:: animal ovaries; apoptosis; calcium; cell cycle; cell lines; cycloaddition reactions; green chemistry; humans; inhibitory concentration 50; liver neoplasms; membrane potential; mitochondrial membrane; neoplasm cells; ovarian neoplasms; pyridines; urinary bladder neoplasms
Abstract:: ... Herein, a transition metal-free and efficient cascade reaction of ynones with 2-methylbenzimidazoles under mild conditions has been revealed. This cascade reaction involved a Michael addition/intramolecular cycloaddition/dehydration and provided the desired benzo[4,5]imidazo[1,2-a]pyridines in moderate to good yield. Furthermore, three benzo[4,5]imidazo[1,2-a]pyridines (3d, 3i, and 3q) exhibited g ...
DOI:: 10.1039/c8gc00069g
: https://doi.org/10.1039/c8gc00069g

63. Two new β-carboline alkaloids from the stems of Picrasma quassioides

Author:: Hyun Sook Kwon; Hanna Lee; Jin Su Lee; Kijun Lee; Jung-Hye Choi; Dae Sik Jang
Source:: Archives of pharmacal research 2018 v.41 no.5 pp. 513-518
ISSN:: 0253-6269
Subject:: Picrasma quassioides; alkaloids; animal ovaries; cell lines; cytotoxicity; humans; nuclear magnetic resonance spectroscopy; ovarian neoplasms; spectral analysis; stems
Abstract:: ... Two new β-carboline alkaloids, 1-acetyl-4-methoxy-8-hydroxy-β-carboline (1) and 1-acetyl-4,8-dimethoxy-β-carboline (2), together with 10 known compounds; seven β-carboline alkaloids (3–9), two canthin-6-one alkaloids (10 and 11), and one quassinoid (12) were isolated from the stems of Picrasma quassioides. The structure of the new compounds 1 and 2 were determined by spectroscopic analyses includi ...
DOI:: 10.1007/s12272-018-1034-2
: https://doi.org/10.1007/s12272-018-1034-2

64. Understanding biopharmaceutical production at single nucleotide resolution using ribosome footprint profiling

Author:: Ioanna Tzani; Craig Monger; Paul Kelly; Niall Barron; Ronan M Kelly; Colin Clarke
Source:: Current opinion in biotechnology 2018 v.53 pp. 182-190
ISSN:: 0958-1669
Subject:: Chinese hamsters; animal ovaries; biopharmaceuticals; cell lines; engineering; high-throughput nucleotide sequencing; manufacturing; monoclonal antibodies; patients; protein synthesis; ribosomes
Abstract:: ... Biopharmaceuticals such as monoclonal antibodies have revolutionised the treatment of a variety of diseases. The production of recombinant therapeutic proteins, however, remains expensive due to the manufacturing complexity of mammalian expression systems and the regulatory burden associated with administrating these medicines to patients in a safe and efficacious manner. In recent years, academic ...
DOI:: 10.1016/j.copbio.2018.01.030
: https://doi.org/10.1016/j.copbio.2018.01.030

65. Verteporfin mediated sequence dependent combination therapy against ovarian cancer cell line

Author:: S. Sibel Erdem; Vildan Akgul Obeidin; Turkan Yigitbasi; S. Serranur Tumer; Pakize Yigit
Source:: Journal of photochemistry and photobiology 2018 v.183 pp. 266-274
ISSN:: 1011-1344
Subject:: animal ovaries; apoptosis; cell lines; cell membranes; neoplasm cells; ovarian neoplasms; oxygen; peptides; photochemotherapy; photosensitizing agents; synergism
Abstract:: ... Ovarian Cancer is one of the deadliest gynecological cancer showing high resistance to chemotherapy. Non-overlapping and synergistic combination therapies are the best option to overcome this multi-pathological silent disease. Cationic peptides (CPs) with high targeting feature and ability to pass through cell membrane induce apoptosis via disruption of cancer cell membrane. Photodynamic Therapy ( ...
DOI:: 10.1016/j.jphotobiol.2018.04.039
: https://doi.org/10.1016/j.jphotobiol.2018.04.039

66. Visualisation of intracellular production bottlenecks in suspension-adapted CHO cells producing complex biopharmaceuticals using fluorescence microscopy

Author:: Sven Mathias; Simon Fischer; René Handrick; Jürgen Fieder; Patrick Schulz; Harald Bradl; Ingo Gorr; Martin Gamer; Kerstin Otte
Source:: Journal of biotechnology 2018 v.271 pp. 47-55
ISSN:: 0168-1656
Subject:: Chinese hamsters; Golgi apparatus; animal ovaries; antibodies; automation; biopharmaceuticals; cell lines; endoplasmic reticulum; fluorescence microscopy; immunoglobulin G; recombinant proteins; secretion; translation (genetics)
Abstract:: ... With the advance of complex biological formats such as bispecific antibodies or fusion proteins, mammalian expression systems often show low performance. Described determining factors may be accumulation or haltering of heterologous proteins within the different cellular compartments disturbing transport or secretion. In case of the investigated bispecific antibody (bsAb)-producing Chinese hamster ...
DOI:: 10.1016/j.jbiotec.2018.02.009
: https://doi.org/10.1016/j.jbiotec.2018.02.009

67. WITHDRAWN: 3,3′-Diindolylmethane suppresses ovarian cancer cell viability and metastasis and enhances chemotherapy sensitivity via STAT3 and Akt signaling in vitro and in vivo

Author:: Minghua Zou; Changhua Xu; Hua Li; Xianquan Zhang; Weidong Fan
Source:: Archives of biochemistry and biophysics 2018 pp. -
ISSN:: 0003-9861
Subject:: animal ovaries; antineoplastic activity; caspase-3; cell lines; cell viability; cisplatin; drug therapy; gene expression; genes; hypoxia-inducible factor 1; metastasis; mice; neoplasm cells; ovarian neoplasms; phosphorylation; pro-apoptotic proteins; protein synthesis; signal transduction; vascular endothelial growth factors
Abstract:: ... This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause.The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal ...
DOI:: 10.1016/j.abb.2018.07.002
: https://doi.org/10.1016/j.abb.2018.07.002

68. The contributions of individual galactosyltransferases to protein specific N-glycan processing in Chinese Hamster Ovary cells

Author:: Nina Bydlinski; Daniel Maresch; Valerie Schmieder; Gerald Klanert; Richard Strasser; Nicole Borth
Source:: Journal of biotechnology 2018 v.282 pp. 101-110
ISSN:: 0168-1656
Subject:: Chinese hamsters; animal ovaries; cell lines; erythropoietin; galactosyltransferases; glycosylation; isozymes; mass spectrometry; models; product quality
Abstract:: ... Galactosylation as part of N-glycan processing is conducted by a set of beta-1,4-galactosyltransferases (B4GALTs), with B4GALT1 as the dominant isoenzyme for this reaction. Nevertheless, the exact contributions of this key-player as well as of the other isoenzymes involved in N-glycosylation, B4GALT2, B4GALT3 and B4GALT4, have not been studied in-depth.To increase the understanding of the protein- ...
DOI:: 10.1016/j.jbiotec.2018.07.015
: https://doi.org/10.1016/j.jbiotec.2018.07.015

69. An efficient method to control high mannose and core fucose levels in glycosylated antibody production using deoxymannojirimycin

Author:: Sagit Shalel Levanon; Orit Aharonovitz; Ayelet Maor-Shoshani; Gita Abraham; Dan Kenett; Yehoshua Aloni
Source:: Journal of biotechnology 2018 v.276-277 pp. 54-62
ISSN:: 0168-1656
Subject:: Chinese hamsters; animal ovaries; antibodies; antibody formation; bioreactors; biosynthesis; cell lines; cell viability; dose response; enzyme inhibitors; fucose; glycosylation; immunoglobulin G; mannose; protein value; therapeutics
Abstract:: ... Glycosylation on the Fc region of recombinant Immunoglobulin G (IgG) therapeutic antibodies is a critical protein quality attribute which may affect the efficacy and safety of the molecule. During the development of biosimilar therapeutics, adjustment of the glycosylation profile is required in order to match the reference innovator profile. Deoxymannojirimycin (DMJ), a known inhibitor of mannosid ...
DOI:: 10.1016/j.jbiotec.2018.04.006
: https://doi.org/10.1016/j.jbiotec.2018.04.006

70. A highly GSH-sensitive SN-38 prodrug with an “OFF-to-ON” fluorescence switch as a bifunctional anticancer agent

Author:: Chang-Hee Whang; Eunsoo Yoo; Seong Kwon Hur; Kyeong Soo Kim; Dongin Kim; Seongbong Jo
Source:: Chemical communications 2018 v.54 no.65 pp. 9031-9034
ISSN:: 1364-548X
Subject:: animal ovaries; antineoplastic agents; cell lines; chemical bonding; chemical compounds; cytotoxicity; drug therapy; electron transfer; fluorescence; metabolites; neoplasm cells; ovarian neoplasms
Abstract:: ... SN-38 (7-ethyl-10-hydroxy-camptothecin) is an active metabolite of irinotecan (CPT-11) and the most potent camptothecin analogue. In this study, 2,4-dinitrobenzene sulfonyl (DNS) was covalently conjugated as a GSH-sensitive trigger to 10’ –OH of SN-38 to yield a GSH-sensitive prodrug, denoted as DNS-SN38, with virtually quenched fluorescence due to donor-excited photo-induced electron transfer (d- ...
DOI:: 10.1039/c8cc05010d
PubMed:: 30047958
PubMed Central:: PMC6112243
: https://doi.org/10.1039/c8cc05010d

71. miR-4478 contributes to the radio-sensitivity of ovarian cancer cells by regulating Fus

Author:: Chunli Ding; Lihui Hou
Source:: Biochemical and biophysical research communications 2018 pp. -
ISSN:: 0006-291X
Subject:: 3' untranslated regions; animal ovaries; cell lines; cell proliferation; gain-of-function mutation; irradiation; luciferase; microRNA; mortality; neoplasm cells; non-coding RNA; ovarian neoplasms; patients; relapse; tissues; women
Abstract:: ... Ovarian cancer is one of the most prevalent cancers among women around the world, with high mortality and relapse rate. MicroRNAs (miRNAs), a class of small non-coding RNAs, have been reported by various studies to regulate multiple biological processes by binding to the 3'UTR of target genes. Radio-resistance is one of the major reasons for treatment failure among ovarian cancer patients. Present ...
DOI:: 10.1016/j.bbrc.2018.12.035
: https://doi.org/10.1016/j.bbrc.2018.12.035

72. pH excursions impact CHO cell culture performance and antibody N-linked glycosylation

Author:: Rubin Jiang; Hao Chen; Sen Xu
Source:: Bioprocess and biosystems engineering 2018 v.41 no.12 pp. 1731-1741
ISSN:: 1615-7591
Subject:: Chinese hamsters; animal ovaries; antibodies; bioreactors; carbon dioxide; cell culture; cell lines; glucose; glycosylation; lactic acid; osmolality; pH
Abstract:: ... pH excursions exist due to frequent base addition and environmental heterogeneity in large-scale bioreactors. Such excursions could lead to suboptimal culture performance. Here we investigated the impact of pH excursions on cell culture performance and N-linked glycosylation for three MAb-producing Chinese hamster ovary cell lines. Frequent pH excursions were introduced by bolus base addition (in ...
DOI:: 10.1007/s00449-018-1996-y
: https://doi.org/10.1007/s00449-018-1996-y

73. “Antimicrobial and antiproliferative activity of essential oil, aqueous and ethanolic extracts of Ocimum micranthum Willd leaves”

Author:: Isabel O. Caamal-Herrera; Leydi M. Carrillo-Cocom; Diana Y. Escalante-Réndiz; Diana Aráiz-Hernández; José A. Azamar-Barrios
Source:: BMC complementary and alternative medicine 2018 v.18 no.1 pp. 55
ISSN:: 1472-6882
Subject:: Bacillus subtilis; Candida albicans; Chinese hamsters; Ocimum campechianum; Pseudomonas aeruginosa; Staphylococcus aureus; alternative medicine; animal ovaries; antimicrobial properties; cell lines; cell proliferation; essential oils; fibroblasts; humans; leaves; mechanism of action; microorganisms; minimum inhibitory concentration; traditional medicine; Mexico
Abstract:: ... BACKGROUND: Ocimum micranthum Willd is a plant used in traditional medicine practiced in the region of the Yucatan peninsula. In particular, it is used for the treatment of cutaneous infections and wound healing, however there are currently no existing scientific studies that support these applications. The aim of the present study was to evaluate the antimicrobial and the in vitro proliferative a ...
DOI:: 10.1186/s12906-018-2122-z
PubMed:: 29422064
PubMed Central:: PMC5806308
: https://doi.org/10.1186/s12906-018-2122-z

74. ALPL regulates the aggressive potential of high grade serous ovarian cancer cells via a non-canonical WNT pathway

Author:: Mu Luo; Lin Zhou; Shi-jie Zhan; Li-juan Cheng; Ruo-nan Li; Hui wang; Bin Liu; Hua Linghu
Source:: Biochemical and biophysical research communications 2019 v.513 no.2 pp. 528-533
ISSN:: 0006-291X
Subject:: animal ovaries; cell lines; cell movement; gene ontology; gene overexpression; genes; genetic disorders; histology; ligands; neoplasm cells; ovarian neoplasms; patients; small interfering RNA
Abstract:: ... The ALPL gene is linked to hypophosphatasia, a rare genetic disease. Owing to the inverse relationships between ALPL expression and both the International Federation of Gynecology and Obstetrics (FIGO) stages and histological grades assigned to patients with serous ovarian cancer (SOC), this study was designed to explore the role and possible mechanisms of ALPL in cell motility of high grade SOC ( ...
DOI:: 10.1016/j.bbrc.2019.04.016
: https://doi.org/10.1016/j.bbrc.2019.04.016

75. Analysis of intracellular IgG secretion in Chinese hamster ovary cells to improve IgG production

Author:: Kohei Kaneyoshi; Keiji Uchiyama; Masayoshi Onitsuka; Noriko Yamano; Yuichi Koga; Takeshi Omasa
Source:: Journal of bioscience and bioengineering 2019 v.127 no.1 pp. 107-113
ISSN:: 1389-1723
Subject:: Chinese hamsters; animal ovaries; antibodies; antibody formation; biopharmaceuticals; cell culture; cell lines; endoplasmic reticulum; engineering; gel chromatography; immunoglobulin G; microscopy; secretion
Abstract:: ... The production of biopharmaceutical immunoglobulin G (IgG) using cultured mammalian cells, especially Chinese hamster ovary (CHO) cells is well established and has been markedly improved through the modification of cells and cell culture engineering technologies. The establishment of high-production cell lines remains a challenge. The intracellular secretion of IgG has been investigated to identif ...
DOI:: 10.1016/j.jbiosc.2018.06.018
: https://doi.org/10.1016/j.jbiosc.2018.06.018

76. Application of a genome-scale model in tandem with enzyme assays for identification of metabolic signatures of high and low CHO cell producers

Author:: Cyrielle Calmels; Solène Arnoult; Bassem Ben Yahia; Laetitia Malphettes; Mikael Rørdam Andersen
Source:: Metabolic Engineering Communications 2019 pp. -
ISSN:: 2214-0301
Subject:: Chinese hamsters; animal ovaries; biopharmaceuticals; cell lines; engineering; glycolysis; lipid metabolism; models; monoclonal antibodies; oxidative phosphorylation; pentose phosphate cycle; phenotype; tricarboxylic acid cycle
Abstract:: ... Biopharmaceutical industrial processes are based on high yielding stable recombinant Chinese Hamster Ovary (CHO) cells that express monoclonal antibodies. However, the process and feeding regimes need to be adapted for each new cell line, as they all have a slightly different metabolism and product performance. A main limitation for accelerating process development is that the metabolic pathways u ...
DOI:: 10.1016/j.mec.2019.e00097
PubMed:: 31720213
PubMed Central:: PMC6838488
: https://doi.org/10.1016/j.mec.2019.e00097

77. Autophagy-dependent toxicity of amino-functionalized nanoparticles in ovarian cancer cells

Author:: Christian Seca; Alessandra Ferraresi; Suratchanee Phadngam; Chiara Vidoni; Ciro Isidoro
Source:: Journal of materials chemistry B 2019 v.7 no.35 pp. 5376-5391
ISSN:: 2050-7518
Subject:: adverse effects; animal ovaries; autophagy; cell lines; chemistry; fluorescence; genes; genetic background; mitochondria; nanoparticles; neoplasm cells; ovarian neoplasms; polystyrenes; proteins; risk; stress response; therapeutics; toxicity
Abstract:: ... The use of nanoparticles (NPs) for diagnostic and therapeutic purposes involves the risk of side effects due to the presence of reactive groups on their surface. We studied the cellular stress response to spheroid fluorescent polystyrene nanoparticles (PS-NPs) functionalized with Amino groups in two ovarian cancer cell lines differing in the expression, among others, of relevant proteins involved ...
DOI:: 10.1039/c9tb00935c
: https://doi.org/10.1039/c9tb00935c

78. BCAT1 and BCAT2 disruption in CHO cells has cell line-dependent effects

Author:: Sara Pereira; Daniel Ley; Mikkel Schubert; Lise Marie Grav; Helene Faustrup Kildegaard; Mikael Rørdam Andersen
Source:: Journal of biotechnology 2019 v.306 pp. 24-31
ISSN:: 0168-1656
Subject:: Chinese hamsters; animal ovaries; branched chain amino acids; byproducts; cell growth; cell lines; essential amino acids; genes; isoleucine; leucine; metabolites; product quality; protein synthesis; recombinant proteins; toxic substances; transferases; valine
Abstract:: ... In recombinant protein expression using Chinese hamster ovary (CHO) cells, chemically defined media contain essential amino acids such as branched chain amino acids (BCAAs) leucine, isoleucine and valine. Availability of amino acids is critical as these are building blocks for protein synthesis. However, breakdown of amino acids can lead to build up of toxic intermediates and metabolites that decr ...
DOI:: 10.1016/j.jbiotec.2019.08.017
: https://doi.org/10.1016/j.jbiotec.2019.08.017

79. Carboplatin prodrug conjugated Fe₃O₄ nanoparticles for magnetically targeted drug delivery in ovarian cancer cells

Author:: Haiqin SongThe two authors contributed equally to this work.; Feifei Quan; Zhiqiang Yu; Minhua Zheng; Yan Ma; Haihua Xiao; Fang Ding
Source:: Journal of materials chemistry B 2019 v.7 no.3 pp. 433-442
ISSN:: 2050-7518
Subject:: adverse effects; animal ovaries; antineoplastic activity; cell lines; chemistry; cisplatin; cytotoxicity; drug resistance; endocytosis; in vivo studies; iron oxides; magnetic fields; nanocarriers; nanoparticles; neoplasm cells; ovarian neoplasms; platinum; toxicity testing; zeta potential
Abstract:: ... Platinum (Pt)-based drugs including cisplatin, carboplatin and oxaliplatin have been widely used as first-line anticancer regimens due to their excellent anticancer efficacy. However, the clinical application of these drugs is greatly limited due to their side effects and drug resistance. In this study, an antitumor drug delivery system based on carboplatin prodrug loading Fe₃O₄ nanoparticles (NPs ...
DOI:: 10.1039/c8tb02574f
: https://doi.org/10.1039/c8tb02574f

80. Cellular Targeting of Bispecific Antibody-Functionalized Poly(ethylene glycol) Capsules: Do Shape and Size Matter?

Author:: Danzi Song; Jiwei Cui; Yi Ju; Matthew Faria; Huanli Sun; Christopher B. Howard; Kristofer J. Thurecht; Frank Caruso
Source:: ACS applied materials & interfaces 2019 v.11 no.32 pp. 28720-28731
ISSN:: 1944-8252
Subject:: Chinese hamsters; animal ovaries; antibodies; breast neoplasms; cell lines; dosimetry; epidermal growth factor receptors; humans; macrophages; mice; mixing; neoplasm cells; polyethylene glycol; surface area
Abstract:: ... In the present study, a capsule system that consists of a stealth carrier based on poly(ethylene glycol) (PEG) and functionalized with bispecific antibodies (BsAbs) is introduced to examine the influence of the capsule shape and size on cellular targeting. Hollow spherical and rod-shaped PEG capsules with tunable aspect ratios (ARs) of 1, 7, and 18 were synthesized and subsequently functionalized ...
DOI:: 10.1021/acsami.9b10304
: https://doi.org/10.1021/acsami.9b10304

81. Cisplatin-loaded PLGA nanoparticles for HER2 targeted ovarian cancer therapy

Author:: Rossina Domínguez-Ríos; Dante R. Sánchez-Ramírez; Kassandra Ruiz-Saray; Paola E. Oceguera-Basurto; Mario Almada; Josué Juárez; Abraham Zepeda-Moreno; Alicia del Toro-Arreola; Antonio Topete; Adrián Daneri-Navarro
Source:: Colloids and surfaces 2019 v.178 pp. 199-207
ISSN:: 0927-7765
Subject:: animal ovaries; breast neoplasms; cell lines; cisplatin; clinical trials; cytotoxicity; drug therapy; epithelium; fluorescein; fluorescence; genetic markers; monoclonal antibodies; multiple drug resistance; nanoparticles; neoplasm cells; ovarian neoplasms; patients; receptors; resistance mechanisms; taxanes; zeta potential
Abstract:: ... The conventional treatment (cytoreduction combined with cisplatin/carboplatin and taxane drugs) of ovarian cancer has a high rate of failure and recurrence despite a favorable initial response. This lack of success is usually attributed to the development of multidrug resistance mechanisms by cancer cells and avoidance of the anti-growth effects of monoclonal targeted therapeutic antibodies. The d ...
DOI:: 10.1016/j.colsurfb.2019.03.011
: https://doi.org/10.1016/j.colsurfb.2019.03.011

82. Comparing two automated high throughput viable-cell counting systems for cell culture applications

Author:: Séverine Fagète; Cédric Steimer; Pierre-Alain Girod
Source:: Journal of biotechnology 2019 v.305 pp. 23-26
ISSN:: 0168-1656
Subject:: Chinese hamsters; animal ovaries; automation; biopharmaceuticals; business enterprises; cell culture; cell lines; cell viability; counters; labor; monitoring
Abstract:: ... Cell counting and viability assessment is an integral part of mammalian cell line development. While manual counting with a hemocytometer is still the gold standard method, its subjectivity and high labor intensity has resulted in its reduced use in favor of automated systems. In addition, some of these automated systems offer multiwell plate based high throughput cell count, which is an asset for ...
DOI:: 10.1016/j.jbiotec.2019.08.014
: https://doi.org/10.1016/j.jbiotec.2019.08.014

83. Differential effects of bioreactor process variables and purification on the human recombinant lysosomal enzyme β-glucuronidase produced from Chinese hamster ovary cells

Author:: Hamideh Parhiz; Stephanie A. Ketcham; Guozhang Zou; Bidesh Ghosh; Erica J. Fratz-Berilla; Muhammad Ashraf; Tongzhong Ju; Chikkathur N. Madhavarao
Source:: Applied microbiology and biotechnology 2019 v.103 no.15 pp. 6081-6095
ISSN:: 0175-7598
Subject:: Chinese hamsters; animal ovaries; beta-glucuronidase; bioreactors; butyrates; capillary electrophoresis; cell lines; drugs; enzyme replacement therapy; matrix-assisted laser desorption-ionization mass spectrometry; molecular models; pH; protein synthesis; purification methods; temperature
Abstract:: ... β-Glucuronidase is a lysosomal enzyme and a molecular model of a class of therapeutics approved as enzyme replacement therapies for lysosomal storage diseases. Understanding the effect of bioreactor process variables on the production and quality of the biologics is critical for maintaining quality and efficacy of the biotherapeutics. Here, we have investigated the effect of three process variable ...
DOI:: 10.1007/s00253-019-09889-7
: https://doi.org/10.1007/s00253-019-09889-7

84. Direct and indirect effects of exposure to 900 MHz GSM radiofrequency electromagnetic fields on CHO cell line: Evidence of bystander effect by non-ionizing radiation

Author:: Najmeh Jooyan; Bahram Goliaei; Bahareh Bigdeli; Reza Faraji-Dana; Ali Zamani; Milad Entezami; Seyed Mohammad Javad Mortazavi
Source:: Environmental research 2019 v.174 pp. 176-187
ISSN:: 0013-9351
Subject:: DNA damage; absorption; adverse effects; animal ovaries; cell death; cell lines; culture media; direct contact; electromagnetic field; energy; hamsters; ionizing radiation; membrane permeability; mitosis; mobile telephones; olives; radio waves; reactive oxygen species
Abstract:: ... The rapid rise in global concerns about the adverse health effects of exposure to radiofrequency radiation (RFR) generated by common devices such as mobile phones has prompted scientists to further investigate the biological effects of these environmental exposures. Non-targeted effects (NTEs) are responses which do not need a direct exposure to be expressed and are particularly significant at low ...
DOI:: 10.1016/j.envres.2019.03.063
: https://doi.org/10.1016/j.envres.2019.03.063

85. Dual-drug loaded micelle for combinatorial therapy targeting HIF and mTOR signaling pathways for ovarian cancer treatment

Author:: Bhuvana S. Doddapaneni; Adel M. AL-Fatease; Deepa A. Rao; Adam W.G. Alani
Source:: Journal of controlled release 2019 v.307 pp. 272-281
ISSN:: 0168-3659
Subject:: anaerobic conditions; angiogenesis; animal models; animal ovaries; apoptosis; cell lines; composite polymers; drug resistance; drugs; evaporation; hypoxia; immunohistochemistry; in vitro studies; mammals; micelles; mutation; ovarian neoplasms; phenotype; polyethylene glycol; rapamycin; remission; signal transduction; solubility; solvents; therapeutics
Abstract:: ... Mutations in the tumor protein (TP53) and the mammalian target of rapamycin (mTOR) pathway have been elucidated as driver mutations in ovarian carcinomas that transform into an invasive phenotype under hypoxic conditions. Chetomin (CHE) targets the hypoxic pathway while Everolimus (EVR) acts on the mTOR pathway. Poor aqueous solubilities of both compounds limit their clinical applications. Diblock ...
DOI:: 10.1016/j.jconrel.2019.06.036
: https://doi.org/10.1016/j.jconrel.2019.06.036

86. Evidence of potential vertical transmission of tilapia lake virus

Author:: Jidapa Yamkasem; Puntanat Tattiyapong; Attapon Kamlangdee; Win Surachetpong
Source:: Journal of fish diseases 2019 v.42 no.9 pp. 1293-1300
ISSN:: 0140-7775
Subject:: RNA; Tilapia lake virus; animal ovaries; biosecurity; breeding stock; cell lines; fish diseases; fish fry; genomics; hatcheries; in situ hybridization; liver; progeny; testes; virus transmission; viruses
Abstract:: ... Tilapia lake virus disease (TiLVD) is an emerging viral disease in tilapia with worldwide distribution. Although the horizontal transmission of TiLV has been demonstrated through the cohabitation of infected fish with susceptible fish, no direct experiment showed the potential of vertical transmission from broodstock to progeny. In this study, natural outbreaks of TiLV in broodstock and fry in two ...
DOI:: 10.1111/jfd.13050
: https://doi.org/10.1111/jfd.13050

87. Expression, purification, and characterization of a human complement component C3 analog that lacks the C-terminal C345c domain

Author:: Kasra X. Ramyar; Xin Xu; Natalie M. White; Andrew Keightley; Brian V. Geisbrecht
Source:: Journal of immunological methods 2019 pp. -
ISSN:: 0022-1759
Subject:: Chinese hamsters; Tobacco etch virus; animal ovaries; cell lines; complement; culture media; gel chromatography; homeostasis; humans; innate immunity; ligands; proteinases; proteolysis; surface plasmon resonance; surface proteins
Abstract:: ... The complement system consists of a series of soluble and cell-surface proteins that serve numerous roles in innate immunity, development, and homeostasis. Despite its many functions, the central event in the complement system is the proteolytic activation of the 185 kDa complement component 3 (C3) into its opsonin and anaphylatoxin fragments known as C3b (175 kDa) and C3a (10 kDa), respectively. ...
DOI:: 10.1016/j.jim.2019.07.005
PubMed:: 31319063
PubMed Central:: PMC6707835
: https://doi.org/10.1016/j.jim.2019.07.005

88. Germinality does not necessarily define mAb expression and thermal stability

Author:: Linda Schwaigerlehner; Patrick Mayrhofer; Matthias Diem; Willibald Steinfellner; Emma Fenech; Chris Oostenbrink; Renate Kunert
Source:: Applied microbiology and biotechnology 2019 v.103 no.18 pp. 7505-7518
ISSN:: 0175-7598
Subject:: Chinese hamsters; amino acid sequences; animal ovaries; cell lines; germ cells; hydrophobicity; loci; models; monoclonal antibodies; protein engineering; somatic mutation; thermal stability
Abstract:: ... The production potential of recombinant monoclonal antibody (mAb) expressing cell lines depends, among other factors, on the intrinsic antibody structure determined by the amino acid sequence. In this study, we investigated the influence of somatic mutations in the V(D)J sequence of four individual, mature model mAbs on the expression potential. Therefore, we defined four couples, each consisting ...
DOI:: 10.1007/s00253-019-09998-3
PubMed:: 31350616
PubMed Central:: PMC6719414
: https://doi.org/10.1007/s00253-019-09998-3

89. Gliotoxin Enhances Autophagic Cell Death via the DAPK1-TAp63 Signaling Pathway in Paclitaxel-Resistant Ovarian Cancer Cells

Author:: Ga-Bin Park; Jee-Yeong Jeong; Daejin Kim
Source:: Marine drugs 2019 v.17 no.7 pp. -
ISSN:: 1660-3397
Subject:: animal ovaries; apoptosis; autophagy; cell lines; death; drug resistance; gene silencing; gliotoxins; marine fungi; membrane potential; mitochondrial membrane; neoplasm cells; ovarian neoplasms; paclitaxel; protein kinases; proteins; signal transduction; transcription (genetics)
Abstract:: ... Death-associated protein kinase 1 (DAPK1) expression induced by diverse death stimuli mediates apoptotic activity in various cancers, including ovarian cancer. In addition, mutual interaction between the tumor suppressor p53 and DAPK1 influences survival and death in several cancer cell lines. However, the exact role and connection of DAPK1 and p53 family proteins (p53, p63, and p73) in drug-resis ...
DOI:: 10.3390/md17070412
PubMed:: 31336860
PubMed Central:: PMC6669733
: https://doi.org/10.3390/md17070412

90. Glyconanoparticles for Targeted Tumor Therapy of Platinum Anticancer Drug

Author:: Aydan Dag; Pinar Sinem Omurtag Ozgen; Sezen Atasoy
Source:: Biomacromolecules 2019 v.20 no.8 pp. 2962-2972
ISSN:: 1526-4602
Subject:: Chinese hamsters; adverse effects; animal ovaries; antineoplastic agents; breast neoplasms; cell lines; composite polymers; cytotoxicity; drug therapy; glycosylation; hydrophobicity; kidney neoplasms; light scattering; moieties; nanocarriers; nanoparticles; neoplasm cells; platinum; polymerization; prostatic neoplasms; toxicity testing; transmission electron microscopy
Abstract:: ... An important requirement to decrease the side effects of chemotherapy drugs is to develop nanocarriers with precise biological functions. In this work, a set of glyconanoparticles was prepared via self-assembly of amphiphilic glycoblock copolymers for the targeted delivery of a hydrophobic chemotherapy drug. Well-defined glycoblock copolymers that consist of 1,1-di-tert-butyl 3-(2-(metyloyloxy)eth ...
DOI:: 10.1021/acs.biomac.9b00528
: https://doi.org/10.1021/acs.biomac.9b00528

91. Host-antibody inductivity of virulent Entamoeba histolytica and non-virulent Entamoeba moshkovskii in a mouse model

Author:: Narumol Khomkhum; Somphob Leetachewa; Aulia Rahmi Pawestri; Saengduen Moonsom
Source:: Parasites & vectors 2019 v.12 no.1 pp. 101
ISSN:: 1756-3305
Subject:: Chinese hamsters; Entamoeba histolytica; adhesion; animal models; animal ovaries; antibodies; cell adhesion; cell lines; colon; cytoskeletal proteins; dysentery; epithelium; humans; immune response; immune system; immunization; immunoglobulin G; liver abscess; membrane proteins; mice; parasites; plasma membrane; protein composition; surface proteins; trophozoites; virulence
Abstract:: ... BACKGROUND: Despite similarities in morphology, gene and protein profiles, Entamoeba histolytica and E. moshkovskii show profound differences in pathogenicity. Entamoeba histolytica infection might result in amoebic dysentery and liver abscess, while E. moshkovskii causes only mild diarrhea. Extensive studies focus on roles of host immune responses to the pathogenic E. histolytica; however, eviden ...
DOI:: 10.1186/s13071-019-3363-5
PubMed:: 30867040
PubMed Central:: PMC6416977
: https://doi.org/10.1186/s13071-019-3363-5

92. Improved recombinant protein production by regulation of transcription and protein transport in Chinese hamster ovary cells

Author:: Kaiming Chen; Dong Li; Hongwen Li; Bing Li; Ruiqin Wang; Lingxiao Jiang; Lei Huang; Xiaoqing Xu; Jie Li; Fei Teng; Cizhong Jiang; Hua Gu; Jianmin Fang
Source:: Biotechnology letters 2019 v.41 no.6-7 pp. 719-732
ISSN:: 0141-5492
Subject:: Chinese hamsters; RNA; animal ovaries; cell lines; gene expression regulation; genes; protein synthesis; protein transport; recombinant proteins; sequence analysis; transcription (genetics); transcriptome
Abstract:: ... OBJECTIVES: To identify genes that affected protein expression in Chinese hamster ovary (CHO) cells was significant, and we identified the changes in the transcriptome and the functional gene sets that would contribute to increase expression of recombinant protein. RESULTS: Here two sub-clones from a methotrexate-treated parental recombinant CHO cell line were selected. The two sub-clones, with di ...
DOI:: 10.1007/s10529-019-02681-7
: https://doi.org/10.1007/s10529-019-02681-7

93. Improving recombinant bone morphogenetic protein-4 (BMP-4) production by autoregulatory feedback loop removal using BMP receptor-knockout CHO cell lines

Author:: Che Lin Kim; Gyun Min Lee
Source:: Metabolic engineering 2019 v.52 pp. 57-67
ISSN:: 1096-7176
Subject:: Chinese hamsters; animal ovaries; autocrine signaling; bone morphogenetic proteins; cell lines; clones; gene editing; gene expression; genes; growth retardation; humans; messenger RNA; methotrexate; signal transduction
Abstract:: ... A Chinese hamster ovary (CHO) cell line producing recombinant human bone morphogenetic protein-4 (rhBMP-4) (CHO-BMP-4), which expresses essential components of BMP signal transduction, underwent autocrine BMP-4 signaling. RNA seq analysis on CHO host cells (DG44) treated with rhBMP-4 (20 µg/mL) suggested that rhBMP-4 induced signaling in CHO cells could be a critical factor in limiting rhBMP-4 pro ...
DOI:: 10.1016/j.ymben.2018.11.003
: https://doi.org/10.1016/j.ymben.2018.11.003

94. In vitro response of human ovarian cancer cells to dietary bioflavonoid isoquercitrin

Author:: Katarina Michalcova; Shubhadeep Roychoudhury; Marek Halenar; Eva Tvrda; Eva Kovacikova; Jaromir Vasicek; Peter Chrenek; Simona Baldovska; Luboslav Sanislo; Vladimir Kren; Adriana Kolesarova
Source:: Journal of environmental science and health 2019 v.54 no.9 pp. 752-757
ISSN:: 1532-4109
Subject:: animal ovaries; apoptosis; cell lines; cell viability; chemiluminescence; dietary supplements; enzyme-linked immunosorbent assay; flow cytometry; isoquercitrin; neoplasm cells; ovarian neoplasms; oxidative stress; reactive oxygen species; transforming growth factor beta 1
Abstract:: ... Isoquercitrin is a dietary bioflavonoid used as a food supplement. We studied the mechanism underlying its effect in human ovarian cancer cells using OVCAR-3 cell line. Viability, survival, apoptosis, release of human transforming growth factor-β1 (TGF-β1) and TGF-β1 receptor, and intracellular reactive oxygen species (ROS) generation by OVCAR-3 cells were examined after isoquercitrin treatment at ...
DOI:: 10.1080/03601234.2019.1633214
: https://doi.org/10.1080/03601234.2019.1633214

95. In vitro selection of DNA aptamers recognizing drug-resistant ovarian cancer by cell-SELEX

Author:: Junqing He; Junyan Wang; Nan Zhang; Luyao Shen; Linlin Wang; Xiao Xiao; Yan Wang; Tao Bing; Xiangjun Liu; Songqing Li; Dihua Shangguan
Source:: Talanta 2019 v.194 pp. 437-445
ISSN:: 0039-9140
Subject:: animal ovaries; blood serum; cell lines; dissociation; drug resistance; glycoproteins; humans; ions; ligands; neoplasm cells; nucleases; nucleotide aptamers; ovarian neoplasms; prognosis; survival rate; temperature; therapeutics
Abstract:: ... Ovarian cancer is regarded as the most lethal gynecologic malignancy with poor prognosis and high mortality rate. Drug-resistance was thought to be the main obstacle to improving overall survival rate of ovarian cancer. New ligands for drug-resistant ovarian cancer cells have potential for the development of diagnosis and therapy of ovarian cancer. In present work, we reported two aptamers, HF3–58 ...
DOI:: 10.1016/j.talanta.2018.10.028
: https://doi.org/10.1016/j.talanta.2018.10.028

96. Increased growth rate and productivity following stable depletion of miR-7 in a mAb producing CHO cell line causes an increase in proteins associated with the Akt pathway and ribosome biogenesis

Author:: Orla Coleman; Srinivas Suda; Justine Meiller; Michael Henry; Markus Riedl; Niall Barron; Martin Clynes; Paula Meleady
Source:: Journal of proteomics 2019 v.195 pp. 23-32
ISSN:: 1874-3919
Subject:: animal ovaries; biogenesis; biopharmaceuticals; cell growth; cell lines; engineering; hamsters; immunoglobulin G; liquid chromatography; microRNA; phenotype; protein synthesis; proteome; proteomics; recombinant proteins; ribosomes; tandem mass spectrometry
Abstract:: ... Cell line engineering using microRNAs represents a desirable route for improving the efficiency of recombinant protein production by CHO cells. In this study we generated stable CHO DP12 cells expressing a miR-7 sponge transcript which sequesters miR-7 from its endogenous targets. Depletion of miR-7 results in a 65% increase in cell growth and >3-fold increase in yield of secreted IgG protein. Qua ...
DOI:: 10.1016/j.jprot.2019.01.003
: https://doi.org/10.1016/j.jprot.2019.01.003

97. Induction of apoptosis in ovarian cancer cells by miR-493-3p directly targeting AKT2, STK38L, HMGA2, ETS1 and E2F5

Author:: Michael Kleemann; Helga Schneider; Kristian Unger; Jeremias Bereuther; Simon Fischer; Philip Sander; E. Marion Schneider; Pamela Fischer-Posovszky; Christian U. Riedel; René Handrick; Kerstin Otte
Source:: Cellular and molecular life sciences 2019 v.76 no.3 pp. 539-559
ISSN:: 1420-682X
Subject:: DNA; animal ovaries; apoptosis; caspases; cell lines; gene expression regulation; luciferase; membrane potential; messenger RNA; microRNA; mitochondrial membrane; neoplasm cells; ovarian neoplasms; phosphorylation; protein synthesis; protein-serine-threonine kinases; proto-oncogenes; serine; signal transduction; threonine; transcription (genetics); transfection
Abstract:: ... Apoptosis is a form of directed programmed cell death with a tightly regulated signalling cascade for the destruction of single cells. MicroRNAs (miRNAs) play an important role as fine tuners in the regulation of apoptotic processes. MiR-493-3p mimic transfection leads to the induction of apoptosis causing the breakdown of mitochondrial membrane potential and the activation of Caspases resulting i ...
DOI:: 10.1007/s00018-018-2958-x
: https://doi.org/10.1007/s00018-018-2958-x

98. Investigation of anti-cancer and migrastatic properties of novel curcumin derivatives on breast and ovarian cancer cell lines

Author:: Jinsha Koroth; Snehal Nirgude; Shweta Tiwari; Vidya Gopalakrishnan; Raghunandan Mahadeva; Sujeet Kumar; Subhas S. Karki; Bibha Choudhary
Source:: BMC complementary and alternative medicine 2019 v.19 no.1 pp. 273
ISSN:: 1472-6882
Subject:: Western blotting; adverse effects; alternative medicine; animal ovaries; antineoplastic activity; apoptosis; bioavailability; breast neoplasms; cell lines; cell movement; curcumin; cytotoxicity; flow cytometry; interstitial collagenase; liquids; metastasis; neoplasm cells; ovarian neoplasms; stem cells; toxicity testing
Abstract:: ... BACKGROUND: Curcumin is known for its multitude of medicinal properties, including anti-cancer and migrastatic activity. Efforts to overcome poor bioavailability, stability, and side effects associated with the higher dose of curcumin has led to the development of newer derivatives of curcumin. Thus, the focus of this study is to screen novel curcumin derivatives, namely ST03 and ST08, which have ...
DOI:: 10.1186/s12906-019-2685-3
PubMed:: 31638975
PubMed Central:: PMC6802352
: https://doi.org/10.1186/s12906-019-2685-3

99. LAMC2 regulated by microRNA-125a-5p accelerates the progression of ovarian cancer via activating p38 MAPK signalling

Author:: Dongya Zhang; Hongjun Guo; Wei Feng; Haifeng Qiu
Source:: Life sciences 2019 v.232 pp. 116648
ISSN:: 0024-3205
Subject:: Western blotting; animal ovaries; apoptosis; bioinformatics; carcinogenesis; cell lines; cell proliferation; flow cytometry; gene expression regulation; gene overexpression; laminin; luciferase; mitogen-activated protein kinase; neoplasm cells; neoplasm progression; oncogenes; ovarian neoplasms; quantitative polymerase chain reaction; staining; tissues
Abstract:: ... Laminin γ2 (LAMC2) is over-expressed in ovarian cancer, and its high expression facilitates cell invasion. Nevertheless, the effects of LAMC2 on other ovarian cancer cell functions and its underlying mechanism remain largely unclear. Bioinformatics analysis shows that LAMC2 is a predicted target of miR-125a-5p and miR-193a-3p. Therefore, the present study aimed to investigate the effects of LAMC2 ...
DOI:: 10.1016/j.lfs.2019.116648
: https://doi.org/10.1016/j.lfs.2019.116648

100. MiR-106b promotes therapeutic antibody expression in CHO cells by targeting deubiquitinase CYLD

Author:: Changzhi Xu; Qianqian Han; Qin Zhou; Lanlan Zhang; Pengfei Wu; Yafang Lu; Yan Si; Tengfei Ma; Bingbing Ma; Buchang Zhang
Source:: Applied microbiology and biotechnology 2019 v.103 no.17 pp. 7085-7095
ISSN:: 0175-7598
Subject:: Chinese hamsters; animal ovaries; antibodies; antibody formation; apoptosis; bioinformatics; cell cycle; cell lines; cell viability; engineering; enzyme inhibition; enzymes; gene overexpression; microRNA; therapeutics; transfection
Abstract:: ... MicroRNAs (miRNAs) function as important regulators of major cellular processes, such as cell cycle, proliferation, development, and apoptosis. Recently, miRNA engineering of Chinese hamster ovary (CHO) cells has emerged as a promising strategy for enhancing therapeutic antibody production. Previously, we have reported that inhibition of deubiquitinase cylindromatosis (CYLD) remarkably enhanced th ...
DOI:: 10.1007/s00253-019-10000-3
: https://doi.org/10.1007/s00253-019-10000-3

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1. β1,6 N-Acetylglucosaminyltransferase (core 2 GlcNAc-T) expression in normal rat tissues and different cell lines: Evidence for complex mechanisms of regulation

2. Oligosaccharide analysis and molecular modeling of soluble forms of glycoproteins belonging to the Ly-6, scavenger receptor, and immunoglobulin superfamilies expressed in Chinese hamster ovary cells

3. Selection strategies for the establishment of recombinant Chinese hamster ovary cell line with dihydrofolate reductase-mediated gene amplification

4. Adhesion and migration of ovarian cancer cells on crosslinked laminin fibers nanofabricated by multiphoton excited photochemistry

5. Platinum(ii) compounds bearing bone-targeting group: synthesis, crystal structure and antitumor activity

6. Conformational and thermal stability of mature dimeric human myeloperoxidase and a recombinant monomeric form from CHO cells

7. Efficient and reproducible mammalian cell bioprocesses without probes and controllers?

8. LSINCT5 is over expressed in breast and ovarian cancer and affects cellular proliferation

9. Computational identification of microRNA gene loci and precursor microRNA sequences in CHO cell lines

10. Identifying the CHO Secretome using Mucin-type O-Linked Glycosylation and Click-chemistry

11. Characterization of Host-Cell Line Specific Glycosylation Profiles of Early Transmitted/Founder HIV-1 gp120 Envelope Proteins

12. Identification of Autocrine Growth Factors Secreted by CHO Cells for Applications in Single-Cell Cloning Media

13. Secretome-Based Identification of TFPI2, A Novel Serum Biomarker for Detection of Ovarian Clear Cell Adenocarcinoma

14. Evaluation of anti-inflammatory and mechanism of action of extract of Macrosiphonia longiflora (Desf.) Müll. Arg

15. Peptides derived from central turn motifs within integrin αIIb and αV cytoplasmic tails inhibit integrin activation

16. Promoting cell adhesion on slippery phosphorylcholine hydrogel surfaces

17. Synthesis and Anticancer Evaluation of Some New 4-Amino-3- (p-methoxybenzyl)-4,5-dihydro-1,2,4-triazole-5-one Derivatives

18. Comparison of SYBR Green and TaqMan real-time PCR methods for quantitative detection of residual CHO host-cell DNA in biopharmaceuticals

19. Conducting polymer nanoparticles for targeted cancer therapy

20. Elucidation of the CHO Super-Ome (CHO-SO) by Proteoinformatics

21. Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells

22. High-Antibody-Producing Chinese Hamster Ovary Cells Up-Regulate Intracellular Protein Transport and Glutathione Synthesis

23. Optimizing production of Fc-amidated peptides by Chinese hamster ovary cells

24. Transcriptome study and identification of potential marker genes related to the stable expression of recombinant proteins in CHO clones

25. The distribution pattern of α2,3- and α2,6-linked sialic acids affects host cell preference in Toxoplasma gondii

26. A synthetic peptide inhibits human ovarian cancer cell motility

27. New halogenated constituents from Mangifera zeylanica Hook.f. and their potential anti-cancer effects in breast and ovarian cancer cells

28. Accumulative scFv-Fc antibody gene integration into the hprt chromosomal locus of Chinese hamster ovary cells

29. CHO cell cytotoxicity and genotoxicity analyses of disinfection by-products: An updated review

30. Evaluating the expression profile and stability of different UCOE containing vector combinations in mAb-producing CHO cells

31. Lessons from the Hamster: Cricetulus griseus Tissue and CHO Cell Line Proteome Comparison

32. LongR3 enhances Fc-fusion protein N-linked glycosylation while improving protein productivity in an industrial CHO cell line

33. Accelerated Barocycler Lysis and Extraction Sample Preparation for Clinical Proteomics by Mass Spectrometry

34. All-trans retinoic acid in combination with sodium butyrate enhances specific monoclonal antibody productivity in recombinant CHO cell line

35. Amelioration of arsenic-induced oxidative stress in CHO cells by Ixora coccinea flower extract

36. Applications of proteomic methods for CHO host cell protein characterization in biopharmaceutical manufacturing

37. A CRISPR/Cas9 based engineering strategy for overexpression of multiple genes in Chinese hamster ovary cells

38. Clonal variation in productivity and proteolytic clipping of an Fc-fusion protein in CHO cells: Proteomic analysis suggests a role for defective protein folding and the UPR

39. Comprehensive Glycoproteomic Analysis of Chinese Hamster Ovary Cells

40. Debottlenecking protein secretion and reducing protein aggregation in the cellular host

41. Development of hyper osmotic resistant CHO host cells for enhanced antibody production

42. Ecotoxicological potential of the biocides terbutryn, octhilinone and methylisothiazolinone: Underestimated risk from biocidal pathways?

43. Engineering a stable CHO cell line for the expression of a MERS-coronavirus vaccine antigen

44. Enhanced production of anti-PD1 antibody in CHO cells through transient co-transfection with anti-apoptotic genes Bcl-x L and Mcl-1

45. Enhancement of glycosylation by stable co-expression of two sialylation-related enzymes on Chinese hamster ovary cells

46. Evaluation of fluorescent dyes to measure protein aggregation within mammalian cell culture supernatants

47. Glyco-Mapper: A Chinese hamster ovary (CHO) genome-specific glycosylation prediction tool

48. In situ base release for pH maintenance can allow shake flasks to better mimic bioreactor performance for CHO cell culture

49. Inactivation of deubiquitinase CYLD enhances therapeutic antibody production in Chinese hamster ovary cells

50. Integrated use of bioinformatic resources reveals that co-targeting of histone deacetylases, IKBK and SRC inhibits epithelial-mesenchymal transition in cancer

51. Intracellular response of CHO cells to oxidative stress and its influence on metabolism and antibody production

52. LncRNA ADAMTS9-AS2 regulates ovarian cancer progression by targeting miR-182-5p/FOXF2 signaling pathway

53. LncRNA LINC00319 accelerates ovarian cancer progression through miR-423-5p/NACC1 pathway

54. Multiple reaction monitoring targeted LC-MS analysis of potential cell death marker proteins for increased bioprocess control

55. PTP1B promotes the malignancy of ovarian cancer cells in a JNK-dependent mechanism

56. Pathological analysis of silkworm infected by two microsporidia Nosema bombycis CQ1 and Vairimorpha necatrix BM

57. Perspectives on progressive strategies and recent trends in the production of recombinant human factor VIII

58. Physiological responses of Chinese hamster ovary cells to a productivity-enhancing yeast extract

59. Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma

60. Rapid development of stable transgene CHO cell lines by CRISPR/Cas9-mediated site-specific integration into C12orf35

61. Strategy for Generating Sequence-Defined Aptamer Reagent Sets for Detecting Protein Contaminants in Biotherapeutics

62. Transition-metal-free C–N and C–C formation: synthesis of benzo[4,5]imidazo[1,2-a]pyridines and 2-pyridones from ynones

63. Two new β-carboline alkaloids from the stems of Picrasma quassioides

64. Understanding biopharmaceutical production at single nucleotide resolution using ribosome footprint profiling

65. Verteporfin mediated sequence dependent combination therapy against ovarian cancer cell line

66. Visualisation of intracellular production bottlenecks in suspension-adapted CHO cells producing complex biopharmaceuticals using fluorescence microscopy

67. WITHDRAWN: 3,3′-Diindolylmethane suppresses ovarian cancer cell viability and metastasis and enhances chemotherapy sensitivity via STAT3 and Akt signaling in vitro and in vivo

68. The contributions of individual galactosyltransferases to protein specific N-glycan processing in Chinese Hamster Ovary cells

69. An efficient method to control high mannose and core fucose levels in glycosylated antibody production using deoxymannojirimycin

70. A highly GSH-sensitive SN-38 prodrug with an “OFF-to-ON” fluorescence switch as a bifunctional anticancer agent

84. Direct and indirect effects of exposure to 900 MHz GSM radiofrequency electromagnetic fields on CHO cell line: Evidence of bystander effect by non-ionizing radiation