PubAg

Main content area

You searched for: Author "Nakayasu, Chihaya" Remove constraint Author: "Nakayasu, Chihaya" Subject antigens Remove constraint Subject: antigens
Start Over

Search

4  Search Results

1 - 4 of 4
Number of results to display per page

Search Results

1. Vibrio anguillarum bacterin uptake via the gills of Japanese flounder and subsequent immune responses

Author:
Nakayasu, Chihaya,   et al.   ; Kato, Goshi; Takano, Tomokazu; Sakai, Takamitsu; Matsuyama, Tomomasa;     Show all 5 Authors
Source:
Fish & shellfish immunology 2013 v.35 no.5 pp. 1591-1597
ISSN:
1050-4648
Subject:
Paralichthys olivaceus; Vibrio anguillarum; adaptive immunity; antigens; epithelial cells; flounder; gene expression regulation; gills; immune response; immunohistochemistry; inflammation; interleukin-6; interleukin-8; serotypes; tumor necrosis factor-alpha
Abstract:
... The mucosal surfaces of fish allow for the introduction of foreign substances, including antigens, from the surrounding environment. In this study, uptake of Vibrio anguillarum J-O-3 serotype bacterin by Japanese flounder, and the subsequent immune responses were investigated. Immunohistochemistry revealed that the bacterin was taken up through the epithelial cells of gills. The transcription leve ...
DOI:
10.1016/j.fsi.2013.09.007

2. Protective efficacies and immune responses induced by recombinant HCD, atpD and gdhA against bacterial cold-water disease in ayu (Plecoglossus altivelis)

Author:
Nakayasu, Chihaya,   et al.   ; Kato, Goshi; Sakai, Takamitsu; Suzuki, Kyuma; Sano, Natsumi; Takano, Tomokazu; Matsuyama, Tomomasa;     Show all 7 Authors
Source:
Fish & shellfish immunology 2014 v.39 no.2 pp. 396-400
ISSN:
1050-4648
Subject:
3-hydroxyacyl-CoA dehydrogenase; Escherichia coli; Flavobacterium psychrophilum; H+/K+-exchanging ATPase; H-transporting ATP synthase; Plecoglossus altivelis; antibodies; antigens; bacterial cold-water disease; blood serum; fish; fluorescent antibody technique; glutamate dehydrogenase; immune response; recombinant proteins; surface proteins; vaccine development
Abstract:
... Protective efficacies of three antigenic proteins (3-hydroxyacyl-CoA dehydrogenase (HCD), ATP synthase beta subunit (atpD), and glutamate dehydrogenase (gdhA)) against Flavobacterium psychrophilum were investigated in ayu (Plecoglossus altivelis). Recombinant proteins of HCD, atpD, and gdhA were expressed in Escherichia coli BL21 cells. Ayu were then vaccinated with inactivated cells via the intra ...
DOI:
10.1016/j.fsi.2014.05.040

3. Antibody profiling using a recombinant protein–based multiplex ELISA array accelerates recombinant vaccine development: Case study on red sea bream iridovirus as a reverse vaccinology model

Author:
Nakayasu, Chihaya,   et al.   ; Matsuyama, Tomomasa; Sano, Natsumi; Takano, Tomokazu; Sakai, Takamitsu; Yasuike, Motoshige; Fujiwara, Atushi; Kawato, Yasuhiko; Kurita, Jun; Yoshida, Kazunori; Shimada, Yukinori;     Show all 11 Authors
Source:
Vaccine 2018 v.36 no.19 pp. 2643-2649
ISSN:
0264-410X
Subject:
Infectious spleen and kidney necrosis virus; Seriola dumerili; Seriola quinqueradiata; antibodies; antigens; blood serum; case studies; enzyme-linked immunosorbent assay; fish; genes; immune response; models; pathogens; prediction; protective effect; recombinant vaccines; screening; sorbents; vaccine development
Abstract:
... Predicting antigens that would be protective is crucial for the development of recombinant vaccine using genome based vaccine development, also known as reverse vaccinology. High-throughput antigen screening is effective for identifying vaccine target genes, particularly for pathogens for which minimal antigenicity data exist. Using red sea bream iridovirus (RSIV) as a research model, we developed ...
DOI:
10.1016/j.vaccine.2018.03.059

4. Enhancement of piscine orthoreovirus-2 DNA vaccine potency by linkage of antigen gene to a trigger factor gene or signal peptide genes

Author:
Nakayasu, Chihaya,   et al.   ; Matsuyama, Tomomasa; Takano, Tomokazu; Honjo, Miho; Kikuta, Takumi; Nawata, Akatsuki; Kumagai, Akira; Honda, Ryo; Sakai, Takamitsu; Kurita, Jun; Terashima, Sachiko; Yamazaki, Masatoshi; Matsuura, Yuta; Nishizawa, Yuko;     Show all 14 Authors
Source:
Aquaculture 2021 v.533 pp. 736163
ISSN:
0044-8486
Subject:
Oncorhynchus kisutch; anemia; antibodies; antigens; antiserum; blood serum; cell lines; erythrocytes; etiological agents; fish; genes; genotype; inclusion bodies; muscles; necrosis; protective effect; recombinant vaccines; signal peptide; vaccination; vaccine development; viral growth; viral load; viral nonstructural proteins; virus replication; Japan
Abstract:
... Coho salmon (Oncorhynchus kisutch) is a Pacific salmon species that is commercially farmed in Japan, and is susceptible to erythrocyte inclusion body syndrome (EIBS), which is characterized by anemia, erythrocyte inclusion bodies, and cardiovascular muscle necrosis. At present, no vaccine for this disease is available. Piscine orthoreovirus-2 (PRV-2) is the causative agent of EIBS in Japan, and is ...
DOI:
10.1016/j.aquaculture.2020.736163