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You searched for: Subject term antiserum Remove constraint Subject term: antiserum Subject term bacterial antigens Remove constraint Subject term: bacterial antigens
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1. Early migration pattern of Avibacterium paragallinarum in the nasal passage of experimentally infected chicken and Japanese quail by immunohistochemistry

Author:
Aadish Balouria; Sidhartha Deshmukh; H. S. Banga; Ajaz Ahmad; R. S. Brar; Sandeep Sodhi
Source:
Avian pathology 2019 v.48 no.2 pp. 168-177
ISSN:
1465-3338
Subject:
Avibacterium paragallinarum; Coturnix japonica; antiserum; bacterial antigens; chickens; farmers; immunoelectrophoresis; immunohistochemistry; microorganisms; nasal cavity; pathogens; polyclonal antibodies; respiratory mucosa; swollen head syndrome; vaccines; virulence
Abstract:
... Infectious coryza (IC) is often a curse for poultry farmers when it occurs concurrently with several pathogens causing swollen head syndrome. The disease is caused by Avibacterium paragallinarum, which inflicts initial damage to the nasal and respiratory epithelium. This facilitates the progression of disease pathology across the nasal cavity, thereby providing a platform for multiplication of opp ...
DOI:
10.1080/03079457.2018.1562153

2. In silico identification and high throughput screening of antigenic proteins as candidates for a Mannheimia haemolytica vaccine

Author:
Cassidy L. Klima; Rahat Zaheer; Shaun R. Cook; Jay Rasmussen; Trevor W. Alexander; Andrew Potter; Steve Hendrick; Tim A. McAllister
Source:
Veterinary immunology and immunopathology 2018 v.195 pp. 19-24
ISSN:
0165-2427
Subject:
ABC transporters; Mannheimia haemolytica; antiserum; bacterial antigens; cattle; cell membranes; enzyme-linked immunosorbent assay; genome; genomics; hemagglutinins; molybdenum; outer membrane proteins; pathogens; porins; protein synthesis; respiratory tract diseases; screening; serine proteinases; serotypes; signal peptide; solutes; transcription (genetics); translation (genetics); vaccines
Abstract:
... This study examined the use of comparative genomic analysis for vaccine design against Mannheimia haemolytica, a respiratory pathogen of ruminants. A total of 2,341genes were identified in at least half of the 23 genomes. Of these, a total of 240 were identified to code for N-terminal signal peptides with diverse sub-cellular localizations (78 periplasmic, 52 outer membrane, 15 extracellular, 13 c ...
DOI:
10.1016/j.vetimm.2017.11.004

3. Identification and characterization of a novel conserved 46 kD maltoporin of Aeromonas hydrophila as a versatile vaccine candidate in European eel (Anguilla anguilla)

Author:
Jianjun Feng; Peng Lin; Songlin Guo; Yuanyuan Jia; Yilei Wang; Frank Zadlock; Ziping Zhang
Source:
Fish & shellfish immunology 2017 v.64 pp. 93-103
ISSN:
1050-4648
Subject:
Aeromonas hydrophila; Anguilla anguilla; Western blotting; affinity chromatography; amino acids; antibodies; antiserum; bacterial antigens; blood cells; blood serum; databases; eel; immune response; intraperitoneal injection; lysozyme; molecular cloning; nickel; nucleotides; open reading frames; outer membrane proteins; sequence alignment; serotypes; vaccines
Abstract:
... European eel (Anguilla anguilla) is a crucial economic fish that has been plagued by Aeromonas hydrophila infections for many years. Vaccines that are cross-protective against multiple serotypes could provide an effective control against A. hydrophila-mediated diseases. The outer membrane proteins (OMPs) are highly immunogenic and capable of eliciting protective immune responses. This study report ...
DOI:
10.1016/j.fsi.2017.03.010

4. Synthesis and immunodetection of 6-O-methyl-phosphoramidyl-α-D-galactose: a Campylobacter jejuni antigenic determinant

Author:
Yuening Jiao; Zuchao Ma; Cheryl P. Ewing; Patricia Guerry; Mario A. Monteiro
Source:
Carbohydrate research 2015 v.418 pp. 9-12
ISSN:
0008-6215
Subject:
Campylobacter jejuni; antiserum; bacterial antigens; diarrhea; galactose; glycosides; immune response; polysaccharides; serotypes; vaccines
Abstract:
... Campylobacter jejuni is a leading cause of traveler's diarrhea. Previously, we have shown that a C. jejuni capsule polysaccharide (CPS) conjugate vaccine can fully prevent C. jejuni diarrhea in non-human primates. C. jejuni CPSs are decorated with non-stoichiometric amounts of O-methyl phosphoramidate (MeOPN) units that are key serospecific markers. In the case of C. jejuni serotype complex HS23/3 ...
DOI:
10.1016/j.carres.2015.09.012

5. Identification of vaccine antigens using integrated proteomic analyses of surface immunogens from serogroup B Neisseria meningitidis

Author:
Nikos Tsolakos; Charlotte Brookes; Stephen Taylor; Andrew Gorringe; Christoph M. Tang; Ian M. Feavers; Jun X. Wheeler
Source:
Journal of proteomics 2014 v.101 pp. 63-76
ISSN:
1874-3919
Subject:
Neisseria meningitidis; Western blotting; antiserum; bacteria; bacterial antigens; bacterial infections; complement; enzyme-linked immunosorbent assay; immune response; macrophages; meningitis; mice; pathogenicity; peptidase K; proteome; proteomics; serotypes; subunit vaccines; surface proteins; two-dimensional gel electrophoresis
Abstract:
... Meningococcal surface proteins capable of evoking a protective immune response are candidates for inclusion in protein-based vaccines against serogroup B Neisseria meningitidis (NmB). In this study, a 2-dimensional (2-D) gel-based platform integrating surface and immune-proteomics was developed to characterize NmB surface protein antigens. The surface proteome was analyzed by differential 2-D gel ...
DOI:
10.1016/j.jprot.2014.02.013

6. Identification of a common immune signature in murine and human systemic Salmonellosis

Author:
Seung-Joo Lee; Li Liang; Silvia Juarez; Minelva R. Nanton; Esther N. Gondwe; Chisomo L. Msefula; Matthew A. Kayala; Francesca Necchi; Jennifer N. Heath; Peter Hart; Renée M. Tsolis; Robert S. Heyderman; Calman A. MacLennan; Philip L. Felgner; D. Huw Davies; Stephen J. McSorley
Source:
Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.13 pp. 4998-5003
ISSN:
0027-8424
Subject:
Salmonella; antigen-antibody reactions; antiserum; bacteremia; bacterial antigens; bacterial proteins; children; humans; humoral immunity; mice; proteome; salmonellosis; vaccination
Abstract:
... Despite the importance of Salmonella infections in human and animal health, the target antigens of Salmonella-specific immunity remain poorly defined. We have previously shown evidence for antibody-mediating protection against invasive Salmonellosis in mice and African children. To generate an overview of antibody targeting in systemic Salmonellosis, a Salmonella proteomic array containing over 2, ...
DOI:
10.1073/pnas.1111413109
PubMed:
22331879
PubMed Central:
PMC3324033

7. Identification of Circulating Bacterial Antigens by In Vivo Microbial Antigen Discovery

Author:
Dana E. Nuti; Reva B. Crump; Farida Dwi Handayani; Narisara Chantratita; Sharon J. Peacock; Richard Bowen; Philip L. Felgner; D. Huw Davies; Terry Wu; C. Rick Lyons; Paul J. Brett; Mary N. Burtnick; Thomas R. Kozel; David P. AuCoin
Source:
mBio 2011 v.2 no.4 pp. e00136
ISSN:
2150-7511
Subject:
Burkholderia pseudomallei; Francisella tularensis; antibodies; antiserum; bacteria; bacterial antigens; bacterial proteins; blood serum; circulating antigens; diagnostic techniques; early diagnosis; immune response; immunoassays; infectious diseases; lipopolysaccharides; mice; microbial detection; pathogens; patients; proteome; therapeutics
Abstract:
... Detection of microbial antigens in clinical samples can lead to rapid diagnosis of an infection and administration of appropriate therapeutics. A major barrier in diagnostics development is determining which of the potentially hundreds or thousands of antigens produced by a microbe are actually present in patient samples in detectable amounts against a background of innumerable host proteins. In t ...
DOI:
10.1128/mBio.00136-11
PubMed:
21846829
PubMed Central:
PMC3163937

8. Microscopic and polyclonal antibody-based detection of yellow leaf disease of arecanut (Areca catechu L.)

Author:
G. Rajeev; V. R. Prakash; M. Mayil Vaganan; M. Sasikala; J. J. Solomon; G. M. Nair
Source:
Archives of phytopathology and plant protection 2011 v.44 no.11 pp. 1093-1104
ISSN:
1477-2906
Subject:
Areca catechu; Phytoplasma; absorption; agar; antiserum; bacterial antibodies; bacterial antigens; centrifugation; disease detection; enzyme-linked immunosorbent assay; gels; leaves; microbial detection; pathogens; polyclonal antibodies; rabbits; scanning electron microscopy; sieve elements; transmission electron microscopy
Abstract:
... Phytoplasma, the pathogen of yellow leaf disease (YLD) of arecanut (Areca catechu L.) was detected by transmission and scanning electron microscopy. Tissues of YLD affected palms contained phytoplasmas in the phloem sieve elements, but not in symptomless healthy palm tissues. Phytoplasma was purified from tissues of diseased palms employing percoll density gradient centrifugation and confirmed by ...
DOI:
10.1080/03235408.2010.482402

9. Protection against heterologous Streptococcus iniae isolates using a modified bacterin vaccine in Nile tilapia, Oreochromis niloticus (L.)

Author:
Craig A. Shoemaker; Benjamin R. LaFrentz; Phillip H. Klesius; Joyce J. Evans
Source:
Journal of fish diseases 2010 v.33 no.7 pp. 537-544
ISSN:
0140-7775
Subject:
Geographical Locations; Oreochromis niloticus; Streptococcus iniae; Western blotting; animal pathogenic bacteria; antigenic variation; antiserum; aquaculture; bacterial antigens; bacterial infections; bacterial proteins; disease prevention; fish; fish diseases; molecular weight; polyacrylamide gel electrophoresis; vaccination; vaccines
Abstract:
... Streptococcus iniae is a significant pathogen impacting aquaculture production worldwide. The objectives of this study were to determine whether a developed modified S. iniae (ARS-98-60) bacterin vaccine is efficacious in Nile tilapia, Oreochromis niloticus (L.), against challenge with heterologous isolates from diverse geographical locations and to evaluate protein and antigenic variability among ...
Handle:
10113/55433
DOI:
10.1111/j.1365-2761.2010.01148.x
PubMed:
20298447

10. Secreted and immunogenic proteins produced by the honeybee bacterial pathogen, Paenibacillus larvae

Author:
Antúnez, Karina; Anido, Matilde; Evans, Jay D.; Zunino, Pablo
Source:
Veterinary microbiology 2010 v.141 no.3-4 pp. 385-389
ISSN:
0378-1135
Subject:
American foul brood; Apis mellifera; Bacillus (bacteria); Paenibacillus larvae; Western blotting; antiserum; apiculture; bacterial antigens; bacterial motility; bacterial proteins; bacterial spores; carbohydrate metabolism; honey bees; immune response; insect larvae; models; pathogens; physiological transport; polyacrylamide gel electrophoresis; polysaccharides; protein folding; protein secretion; toxicity; transcription (genetics); translation (genetics); virulence
Abstract:
... American Foulbrood is a severe disease affecting larvae of honeybee Apis mellifera, causing significant decrease in the honeybee population, beekeeping industries and agricultural production. In spite of its importance, little is known about the virulence factors secreted by Paenibacillus larvae during larval infection. The aim of the present work was to perform a first approach to the identificat ...
Handle:
10113/41239
DOI:
10.1016/j.vetmic.2009.09.006

11. Comparison of antigenic proteins from Lactococcus garvieae KG (−) and KG (+) strains that are recognized by olive flounder (Paralichthys olivaceus) antibodies

Author:
Shin, Gee-Wook; Nho, Seong-Won; Park, Seong-Bin; Jang, Ho-Bin; Cha, In-Seok; Ha, Mi-Ae; Kim, Young-Rim; Dalvi, Rishikesh S.; Joh, Seong-Joon; Jung, Tae-Sung
Source:
Veterinary microbiology 2009 v.139 no.1-2 pp. 113-120
ISSN:
0378-1135
Subject:
heat shock proteins; Paralichthys olivaceus; proteome; antigenic variation; antibody formation; biomarkers; serotypes; flounder; strain differences; proteomics; bacterial antigens; fish diseases; Lactococcus garvieae; bacterial proteins; antiserum; pathotypes; antigen-antibody reactions; virulence; bacterial infections
Abstract:
... Lactococcus garvieae is an important etiological agent of lactococcosis in various fish species including olive flounder (Paralichthys olivaceus). In this study, proteomic and immunoproteomic analyses were employed to compare the antigenic profiles of strains KG9408, MS93003, and NSS9310 strains of L. garvieae. Proteomic analysis using two-dimensional gel electrophoresis (2-DE) revealed difference ...
DOI:
10.1016/j.vetmic.2009.05.007

12. The Major Neutralizing Antibody Responses to Recombinant Anthrax Lethal and Edema Factors Are Directed to Non-Cross-Reactive Epitopes

Author:
Nguyen, Melissa L.; Terzyan, Simon; Ballard, Jimmy D.; James, Judith A.; Farris, A. Darise
Source:
Infection and immunity 2009 v.77 no.11 pp. 4714-4723
ISSN:
0019-9567
Subject:
B-lymphocytes; amino acids; anthrax; antiserum; bacterial antigens; bacterial toxins; enzyme-linked immunosorbent assay; epitopes; humoral immunity; immunoglobulin G; mice; neutralization; neutralizing antibodies; spores; vaccination
Abstract:
... Anthrax lethal and edema toxins (LeTx and EdTx, respectively) form by binding of lethal factor (LF) or edema factor (EF) to the pore-forming moiety protective antigen (PA). Immunity to LF and EF protects animals from anthrax spore challenge and neutralizes anthrax toxins. The goal of the present study is to identify linear B-cell epitopes of EF and to determine the relative contributions of cross- ...
DOI:
10.1128/IAI.00749-09
PubMed:
19720758
PubMed Central:
PMC2772542

13. A DNA Fusion Vaccine Induces Bactericidal Antibodies to a Peptide Epitope from the PorA Porin of Neisseria meningitidis

Author:
Zhu, Delin; Williams, Jeannette N.; Rice, Jason; Stevenson, Freda K.; Heckels, John E.; Christodoulides, Myron
Source:
Infection and immunity 2008 v.76 no.1 pp. 334-338
ISSN:
0019-9567
Subject:
Neisseria meningitidis; antibacterial properties; antibodies; antiserum; bacteria; bacterial antigens; bacterial vaccines; enzyme-linked immunosorbent assay; epitopes; fluorescent antibody technique; immune response; immunization; mice; plasmids; porins; recombinant vaccines; serotypes; tetanus
Abstract:
... An experimental DNA plasmid vaccine was developed based on a well-characterized and protective peptide epitope derived from a bacterial porin protein. For this study, we used the P1.16b serosubtype epitope, located in variable region (VR)2 in loop 4 of the PorA outer membrane (OM) porin from Neisseria meningitidis serogroup B strain MC58. A plasmid that encoded the entire loop (pPorAloop4) was pre ...
DOI:
10.1128/IAI.00943-07
PubMed:
17967859
PubMed Central:
PMC2223639

14. Production of polyclonal antibodies against spores of Clostridium tyrobutyricum, a contaminant affecting the quality of cheese: characterisation of the immunodominant protein

Author:
Lavilla, Maria; Luis, Ruth de; Conesa, Celia; Perez, Maria D.; Calvo, Miguel; Sanchez, Lourdes
Source:
Food and agricultural immunology 2008 v.19 no.1 pp. 77-91
ISSN:
0954-0105
Subject:
Clostridium tyrobutyricum; bacterial spores; bacterial antigens; enzyme-linked immunosorbent assay; polyclonal antibodies; antiserum; rabbits; chaperonins; ultrasonic treatment
DOI:
10.1080/09540100801956653

15. Molecular cloning of a Mycoplasma meleagridis-specific antigenic domain endowed with a serodiagnostic potential

Author:
Ben Abdelmoumen Mardassi, B.; Bejaoui Khiari, A.; Oussaief, L.; Landoulsi, A.; Brik, C.; Mlik, B.; Amouna, F.
Source:
Veterinary microbiology 2007 v.119 no.1 pp. 31-41
ISSN:
0378-1135
Subject:
bacteriophages; bacterial antigens; epitopes; interspecific variation; recombinant antigens; Mycoplasma meleagridis; gene expression; membrane proteins; antiserum; molecular weight; screening; Escherichia coli; disease detection; antigen detection; pathogen identification; molecular epidemiology; molecular cloning; serodiagnosis; poultry diseases; genomics
Abstract:
... A recombinant phage library harbouring Mycoplasma meleagridis (MM) genomic DNA fragments was generated in the bacteriophage lambda gt11 expression vector. The library was screened for expression of MM specific antigens with a polyclonal antiserum that had been preadsorbed with antigens of the most common unrelated avian mycoplasma species. A 49-aminoacid antigenic domain unique to MM was isolated, ...
DOI:
10.1016/j.vetmic.2006.08.008

16. Variation in the molecular weight of Photobacterium damselae subsp. piscicida antigens when cultured under different conditions in vitro

Author:
Jung, T.S.; Thompson, K.D.; Volpatti, D.; Galeotti, M.; Adams, A.
Source:
Journal of veterinary science 2007 v.8 no.3 pp. 255-261
ISSN:
1229-845X
Subject:
Photobacterium damselae subsp. piscicida; bacterial antigens; molecular weight; in vitro culture; culture media; iron; antigen-antibody reactions; antiserum; Dicentrarchus labrax; fish diseases; pasteurellosis; enzyme-linked immunosorbent assay; Western blotting
DOI:
10.4142/jvs.2007.8.3.255
PubMed:
17679772
PubMed Central:
PMC2868132

17. Anti-capsular antibodies activate killing of Escherichia coli O8:K87 by the alternate complement pathway in porcine serum

Author:
Clark, N.M.; Berberov, E.M.; Wang, M.; Moxley, R.A.
Source:
Veterinary immunology and immunopathology 2006 v.114 no.1-2 pp. 185-191
ISSN:
0165-2427
Subject:
complement; blood serum; food animals; antibodies; serotypes; bacterial antigens; enterotoxigenic Escherichia coli; food pathogens; affinity chromatography; immune response; immunization; antiserum; swine
Abstract:
... Enterotoxigenic Escherichia coli (ETEC) strains that produce K88 (F4)+ fimbria are important causes of diarrhea and post-diarrheal septicemia in swine. ETEC O8:K87, a serotype represented by a number of these strains, is typically serum resistant. Strain-specific antibodies are known to activate alternative C pathway-mediated killing of other serum-resistant E. coli [Hill, A.W., Shears, A.L., Hibb ...
DOI:
10.1016/j.vetimm.2006.07.008

18. An Improved Immunohistochemical Diagnostic Technique for Canine Leptospirosis using Antileptospiral Antibodies on Renal Tissue

Author:
Chad J. Wild; Justin J. Greenlee; Carole A. Bolin; Jeanne K. Barnett; A. David Haake; Norman F. Cheville
Source:
Journal of veterinary diagnostic investigation v.14 no.1 pp. 20-24
ISSN:
1040-6387
Subject:
diagnostic techniques; immunoglobulin G; leptospirosis; immunohistochemistry; antibodies; dogs; bacterial antigens; membrane proteins; nephritis; kidneys; antiserum
Abstract:
... The purpose of this study was to compare the immunoreactivity in canine renal tissues stained with antisera specific for 3 leptospiral antigens and those processed with traditional staining methods. In addition, immunoglobulin staining was done on tissues with immunoreactivity to leptospiral antigens. Formalin-fixed renal sections from 12 dogs with chronic interstitial nephritis suspected or prove ...
Handle:
10113/18648
DOI:
10.1177/104063870201400105
PubMed:
12680639
PubMed Central:
PMC2666280

19. Identification of two core types in lipopolysaccharides of Actinobacillus pleuropneumoniae representing serotypes 1 to 12

Author:
Mario Jacques; Stéphane Rioux; Sonia-Élaine Paradis; Caroline Bégin; Marcelo Gottschalk
Source:
Canadian journal of microbiology 1996 v.42 no.8 pp. 855-858
ISSN:
0008-4166
Subject:
Actinobacillus pleuropneumoniae; genetic variation; lipopolysaccharides; bacterial antigens; immunoblotting; polyacrylamide gel electrophoresis; physicochemical properties; antiserum
Abstract:
... Lipopolysaccharides (LPS) of Actinobacillus pleuropneumoniae were separated by Tricine–SDS–polyacrylamide gel electrophoresis, which has been shown to improve resolution of low-molecular-mass fast migrating bands. Strains representing the 12 serotypes of A. pleuropneumoniae can be divided in two groups according to the gel mobility of the core–lipid A region of their LPS. The first electromorphic ...
DOI:
10.1139/m96-108
PubMed:
8776854

20. An enzyme-linked immunosorbent assay (ELISA) for monitoring rodent colonies for Pasteurella pneumotropica antibodies

Author:
R. Boot; H. C. W. Thuis; J. L. Veenema; R. G. H. Bakker
Source:
Laboratory animals 1995 v.29 no.3 pp. 307-313
ISSN:
1758-1117
Subject:
cross reaction; Actinobacillus; antibodies; Haemophilus; enzyme-linked immunosorbent assay; bacterial antigens; antiserum; Pasteurella pneumotropica; rodents
Abstract:
... An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Pasteurella pneumotropica antibodies in the sera of rats} mice, hamsters and Mastomys. P. pneumotropica from mice and rats showed cross-reactivity. The ELISA using P. pneumotropica NCTC 8284 detected more infected animals than selective culture in groups of rodents from which P. pneumotropica, Haemophilus sp and/or Act ...
DOI:
10.1258/002367795781088306
PubMed:
7564216

21. Serological studies of Corynebacterium kutscheri and coryneform bacteria using an enzyme-linked immunosorbent assay (ELISA)

Author:
R. Boot; H. Thuis; R. Bakker; J. L. Veenema
Source:
Laboratory animals 1995 v.29 no.3 pp. 294-299
ISSN:
1758-1117
Subject:
cross reaction; Arcanobacterium pyogenes; antibodies; Rhodococcus equi; mice; bacterial antigens; enzyme-linked immunosorbent assay; Corynebacterium; antiserum; rats
Abstract:
... An enzyme-linked immunosorbent assay (ELISA) to measure Corynebacterium kutscheri antibodies in mice and rats was developed. Seven c. kutscheri isolates showed considerable serological relationship, but Japanese isolates differed from the British isolates. The ELISA appeared specific since c. kutscheri antigen did not react with antisera against 8 heterologous coryneform species. Antibodies to c. ...
DOI:
10.1258/002367795781088351
PubMed:
7564214

22. Interlaboratory comparison of enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence (IIF) for detection of Bordetella bronchiseptica antibodies in guineaplgs

Author:
M. Wullenweber; R. Boot
Source:
Laboratory animals 1994 v.28 no.4 pp. 335-339
ISSN:
1758-1117
Subject:
bacteria; guinea pigs; Bordetella bronchiseptica; cell culture; fluorescent antibody technique; antibodies; bacterial antigens; cross reaction; antiserum; enzyme-linked immunosorbent assay
Abstract:
... Thirty-nine guineapigs were examined for Bordetella bronchiseptica infection by culture and serology, using the enzyme-linked immunosorbent assay (ELISA)and the indirect immunofluorescence (IIF) test. Each serological method detected evidence of B. bronchiseptica infection in more guineapigs than did culture. IIF using an antigen prepared from a mouse isolate of B. bronchiseptica detected fewer in ...
DOI:
10.1258/002367794780745056
PubMed:
7830373