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... Cryo-isolation of therapeutic cells such as pancreatic islets has been proposed as a novel new alternative to conventional enzymatic digestion of the pancreas (Taylor, Baicu., Transpl Proc. 43 (2011) 3181–3183. And Cell Transpl. 2013 (In Press)). Apart from avoiding the expense, inconsistencies and toxicity associated with the collagenase-digestion process, cryo-isolation embodies several inherent ...
E. S. C. Camela; R. P. Nociti; V. J. C. Santos; B. I. Macente; G. S. Maciel; M. A. R. Feliciano; W. R. R. Vicente; I. Gill; P. M. Bartlewski; M. E. F. Oliveira
... Spectral Doppler (velocimetric) indices are important indicators of blood perfusion and physiological status of internal organs. Doppler ultrasound is used in andrology to assess the blood flow in the testicular arteries, aiding in the diagnosis of scrotal disorders and abnormal spermatogenesis. It was proposed that blood flow indices measured in the testicular artery, namely, blood flow resistanc ...
... The proliferation of vascular smooth muscle cells (VSMCs) induced by injury to the intima of arteries is an important etiologic factor in vascular proliferative disorders such as atherosclerosis and restenosis. 2,3,4′,5‐Tetrahydroxystilbene‐2‐O‐β‐d‐glucoside (TSG), an active component extracted from Polygonum multiflorum, has been found to have an antiatherosclerotic effect. The aim of this study ...
... Deposition of lipids in the intima is followed by infiltration of inflammatory cells, like monocytic cells and T lymphocytes, in atherosclerosis. However, roles of lipids in the infiltration of the inflammatory cells are not clearly defined. We investigated the possible involvement of cholesterol or its catabolites in recruitment of monocytic cells. Consumption of a high cholesterol-diet resulted ...
... We attempted to determine the effects of a milieu rich in cholesterol molecules on expression of chemokine CXCL8. A high-cholesterol diet led to an increased transcription of the IL-8 gene in the arteries and elevated levels of CXCL8 in sera of ApoE−/− mice, compared with those of wild-type C57BL/6 mice. Treatment of THP-1 monocyte/macrophage cells with 27-hydroxycholesterol (27OHChol) resulted in ...
... Tissue preparation is the key to a successful MALDI mass spectrometry imaging experiment. A number of different tissue preparations methods have recently been reported for increased sensitivity and/or high spatial resolution analysis. In order to better benchmark these methods in terms of the information content and their suitability for analyzing small tissues containing small but distinct region ...
... With an objective to record the appearance, vascularity changes in umbilicus, placentomes, and blood flow to the uterus, adult (age 5-10 yr) Surti buffalo (parity=2-6; n=24) were inseminated during natural oestrus during the breeding season and scanned by transrectal B mode and colour flow mode ultrasonography every 3-4 days till Day 90 of gestation and then at an interval of 15 days for the compl ...
... During a survey on Myxobolus infection of pond-cultured common carp in Syria three Myxobolus spp. were found. Myxobolus dispar infected the gill arteries, forming large elongated plasmodia in the gill filaments. The plasmodia of M. basilamellaris were located in the gill arches at the base of the filaments. Elongated filiform plasmodia of M. encephalicus were fou ...
... OBJECTIVE: To evaluate the feasibility of a Carrel patch method in feline renal transplantation. STUDY DESIGN: Descriptive case series. ANIMALS: Nine healthy donor cats and 9 client recipient cats with chronic renal failure. METHODS: Renal transplantation was performed in 9 cats with chronic renal failure after collection of a donor's left kidney with a Carrel patch technique. A patch of donor aor ...
... The clinically oriented canine elbow anatomy in its complexity earned a high importance in surgery especially after multiple imaging modalities have been used in the benefit of diagnosis and treatment of canine elbow disorders. The bony, joint, and muscular structures, the arteries, the veins and the nerves supplying the elbow are described and illustrated in textbooks and atlases in the context o ...
RADCLIFFE, CATHERINE H.; WOODIE, J. BRETT; HACKETT, RICHARD P.; AINSWORTH, DOROTHY M.; ERB, HOLLIS N.; MITCHELL, LISA M.; SODERHOLM, L. VINCE; DUCHARME, NORM G.
... To compare upper airway mechanics, arterial blood gases, and tracheal contamination in horses with induced left laryngeal hemiplegia (recurrent laryngeal neuropathy [RLN]) treated by laryngoplasty/vocal cordectomy (LPVC) or modified partial arytenoidectomy (MPA). Repeated measures under the following conditions: Control, RLN, LPVC, and MPA. Six horses. Two trials were conducted under all condition ...
arteries; blood flow; magnetic fields; mathematical models; shear stress
Abstract:
... This paper discuss the biomagnetic fluid aspect of blood flow through narrow tubes with mild stenosis. The blood flow in narrow arteries is treated as two-fluid model with non-Newtonian Jeffrey fluid in the core region and in peripheral region as Newtonian fluid. An analytical solution is obtained for the velocity, volume flow rate, and wall shear stress. The effect of Hartmann number, Jeffrey flu ...
... As the backbone and arteries of a comprehensive transportation network, highways play an important role in improving people’s living standards and promoting economic growth. However, globally, there is limited quantifiable data evaluating the highway traffic state, characteristics, and performance. From the 1960s to the present, remote sensing has been regarded as the most effective technology for ...
... In this study, we present a cat with a common 1‐cm‐long celiacomesenteric trunk. The arteria hepatica branched off first from the separate arteria celiaca and gave rise to the arteria gastrica dextra and arteria gastroduodenalis. The main stem of the arteria celiaca then split into the arteria gastrica sinistra and the arteria lienalis. The arteria mesenterica cranialis extended ventrocaudally in ...
... The adult mammalian heart is minimally regenerative after injury, whereas neonatal hearts fully recover even after major damage. New work from the Red-Horse and Woo labs (Das et al., 2019) shows that collateral artery formation is a key mechanism contributing to successful regeneration in newborn mice and provides insights into how collateral arteries form. ...
... To minimize radial compliance mismatch between native arteries and vascular prostheses over the entire blood pressure range, a biomimetic woven prosthesis sample with a bilayer wall has been developed. Poly(trimethylene terephthalate) (PTT) filaments were used as the circumferential yarns in the inner layer to increase the radial compliance of the inner wall, and poly(ethylene terephthalate) (PET) ...
arteries; biodegradability; carrageenan; crosslinking; doxorubicin; enzymes; hepatoma; hydrophilicity; image analysis; iron oxides; magnetic resonance imaging; microparticles; nanoparticles; sulfates
Abstract:
... Trans-arterial chemoembolization (TACE) has been a mainstream treatment for hepatocellular carcinoma (HCC), accordingly there are more requirements for embolic agents’ properties, mainly concentrated on biodegradability and imaging function. A new kind of biodegradable multifunctional porous microspheres (BMPMs) with internal coarse plications are ingeniously designed and manufactured, consisting ...
... Tropoelastin is the dominant monomer that assembles to form elastin, which confers elasticity to vertebrate elastic tissues including skin, arteries, and lungs. Tropoelastin interacts with cells through cell surface receptors including integrins and glycosaminoglycans (GAGs). As the region 17–18 is recognized as a key region in cell attachment and spreading, we utilized C‐terminal truncated tropoe ...
... Evidence suggests that obesity can contribute to the development of certain complications such as insulin resistance, dyslipidemia, accumulation of visceral fats and cardiovascular dysfunctions. Excess fat also blocks small blood vessels and arteries by accumulating plaque, which increases vascular inflammation, atherosclerosis and risk of stroke and other cardiovascular disease. In 2010, the Worl ...
... Hemodynamic perturbations, partly resulting from abnormal vasoconstriction of digital vessels, have been implicated in the pathogenesis of bovine and equine laminitis. This study compared the responsiveness of isolated bovine (BDA) and equine (EDA) digital arteries to pharmacological agents that stimulate receptor systems involved in the regulation of normal vessel tone. The role of the endotheliu ...
... To test the hypothesis that subarachnoid administration of buprenorphine and lidocaine provides more intense and longer lasting perioperative analgesia with less side effects than xylazine and lidocaine in goats. Randomized, blinded, controlled study. Ten healthy female goats randomly assigned to two groups of five animals each. After sedation with acepromazine (0.1 mg kg⁻¹) intravenously (IV), li ...
... AIMS: To determine if abdominal insufflation with medical air will improve oxygenation and ventilation parameters when compared to insufflation with CO₂ in xylazine-sedated sheep undergoing laparoscopic artificial insemination (AI). METHODS: Forty-seven sheep underwent oestrus synchronisation and were fasted for 24 hours prior to laparoscopic AI. Each animal was randomised to receive either CO₂ or ...
... Blood pressure assessment is valuable during management of chronic conditions with increased risk of developing hypertension and as a standard practice for anesthetic monitoring. Normal arterial blood pressure values have not been well described in megachiropteran species. Following anesthetic induction and maintenance with isoflurane in oxygen, arterial blood pressure was obtained from the poster ...
... Birds are an enigma: their plasma glucose concentration is 1.5–2 times higher than similar-sized mammals, yet they do not normally exhibit symptoms of diabetes. We hypothesized that feeding adult mourning doves a refined carbohydrate diet (white bread: WB) for four weeks would raise plasma glucose concentrations and alter metabolic pathways and endothelial function when compared to birds receiving ...
... While there is strong evidence supporting retinal vascular pattern as a distinctive marker for sheep, it would be advantageous to get an insight into its robustness; in other words, to determine whether retinal recognition of young animals (lambs) can reach as good a matching performance as the one demonstrated for adult sheep. To this aim, a longitudinal study was devised to observe the evolution ...
... We describe a method for the isolation and recirculating perfusion of rabbit livers. Livers were perfused simultaneously through the portal vein and the hepatic artery. Precise descriptions of the surgical preparation, perfusion apparatus and perfusion method are given. The viability of rabbit livers was assessed with the use of physiological, biochemical and histological parameters. We conclude t ...
... Perinatal cerebral ischemia is a major cause of brain damage in the human newborn. Animal models of the brain ischemia provided important roles for extension of the pathophysiological knowledge and evaluating of the efficacy of therapeutic interventions. The purpose of the present study was to introduce a cerebral ischemia model of rabbit neonates which provides useful clues for assessing the isch ...
... Polytetrafluorethylene (PTFE) prostheses were implanted in 12 sheep as a shunt between the carotid artery and the jugular vein using an end-to-side anastomosis technique. This technique allows repeated tests of the pharmacological and toxicological safety of artificial kidney units after both single and multiple administration. Further-more, it enables the investigation of detoxification of compou ...
... Bioengineering approaches have been intensively applied to create small diameter vascular grafts using artificial materials. However, a fully successful, high performing and anti-thrombogenic structure has not been achieved yet. In this study, we present the first step of a process aiming at biofunctionalizing previously designed compliant polyethylene terephthalate (PET) scaffolds (Moreno et al., ...
... The cutaneous distribution and anatomical landmarks of the lateral thoracic artery in the dog are reported. Perfusion studies in cadavers showed a variable distribution, but a skin flap capable of rotation through 90° could be created. The successful use of an axial pattern skin flap based on the lateral thoracic artery for reconstruction of a major skin deficit over the caudal elbow of a Greyhoun ...
EDTA (chelating agent); Western blotting; actin; antibodies; arteries; atherosclerosis; blood serum; confocal microscopy; copper sulfate; cytochalasin D; humans; immunoglobulin G; ionization; ligands; liquid chromatography; low density lipoprotein; macrophages; models; monocytes; oxidants; phagocytosis; phenotype; polyacrylamide gel electrophoresis; polymerization; protein kinase C; staining; tandem mass spectrometry; trypsin
Abstract:
... The human monocyte cell line U937 was differentiated into an adherent macrophage phenotype using phorbol 12-myristate 13-acetate (PMA) to assay the phagocytosis of oxidized low-density lipoprotein (oxLDL) that may play a role in atherosclerosis. Microbeads were coated with the inflammatory ligand oxLDL to create a novel phagocytosis assay that models the binding of macrophages to oxLDL in the soli ...
... The ear vessels in the rabbit appear to be easily accessible for blood puncturing. Several measures are described to overcome problems of vascular spasm and uncontrolled movements of the animal during injection. ...
... Objectives: To investigate the prevalence and progression of murmurs and the development of the left ventricular outflow tract (LVOT) and flow velocities in the great arteries in boxer puppies through adolescence until three years of age. Methods: Nineteen boxer puppies with or without a low‐intensity murmur, but without evidence of congenital heart disease, were studied prospectively. Examination ...
... The assessment of collagen content in tissues is important in biomedical research, since this protein is altered in numerous diseases. Hydroxyproline and Sirius red based assays are the most common methods for collagen quantification. However, these procedures have some pitfalls, such as the requirement of oxygen-free medium or expensive equipment and large sample size or being unsuitable for hydr ...
Markov chain; arteries; data collection; development planning; development projects; models; remote sensing; vegetation types; wetlands; Uganda
Abstract:
... This article reports an assessment of the spatial and temporal loss of wetlands to development projects (DPs) in the Kampala–Mukono Corridor (KMC). Four sets of satellite images (1974–2013), historical and field data sets were used to assess changes in the spatial extent of wetland land cover types. Through unsupervised classification, wetland cover types and DPs were delineated and future wetland ...
... OBJECTIVES: To describe the clinical practice of insertion of arterial catheters in anesthetized dogs and cats, to document complications of arterial catheterization, and to determine risk factors associated with the complications. DESIGN: Prospective clinical study and retrospective evaluation of medical records. SETTING: University teaching hospital. ANIMALS: Dogs (n = 251) and 13 cats anestheti ...
... Our team has previously reported a high strength thermoplastic supramolecular polymer hydrogel. However, the hydrogel required injection temperatures outside the physiological range therefore preventing its use in a living environment. In this article, we reported a thermoresponsive supramolecular copolymer hydrogel p(N-acryloyl glycinamide-co-acrylamide) (PNAGA-PAAm), which can be injected at tem ...
... To investigate relevant indicators of quality of care in a large population-based sample of people with diabetes representative of clinical practice in Italy in 2018.We analyzed data from 11,300,750 subjects. All administrative healthcare claims collected in 2018 were scrutinized to identify subjects with diabetes and investigate several indicators of quality of care. Subjects with diabetes were i ...
... Cryosurgery is evolving from a focal therapy for cancer to one that can also treat cardiovascular and neural targets. For example, new focal therapeutic targets include the pulmonary vein (atrial fibrillation), arteries (renal denervation) and nerves (pain and plantar fasciitis). For cryosurgery to successfully compete with heat or other ablative technologies in these new targets, it will be neces ...
... Abnormal levels of High temperature requirement A1 (HtrA1) protein have been repeatedly observed in sera and placentas of preeclampsia patients. To understand the functions of HtrA1 in placentation and in the etiology of preeclampsia, we established HtrA1⁻/⁻ mice. HtrA1⁻/⁻ mice show intrauterine growth retardation, and their placentas are small due to a reduced size of the junctional zone and aber ...
... The Eya1 gene encodes a transcriptional co-activator that acts with Six1 to control the development of different organs. However, Six1-Eya1 interactions and functional roles in mesenchymal cell proliferation and differentiation as well as alveolarization during the saccular stage of lung development are still unknown. Herein, we provide the first evidence that Six1 and Eya1 act together to regulat ...
... Incomplete endothelialization and neointimal hyperplasia of injured arteries can cause acute and late stent thromboses. This work develops hybrid stent/biodegradable nanofibers for the local and sustained delivery of rosuvastatin to denuded artery walls. Biodegradable nanofibers were firstly prepared by dissolving poly(D,L)-lactide-co-glycolide and rosuvastatin in 1,1,1,3,3,3-hexafluoro-2-propanol ...
... Two beef steers accidentally injected into a branch of the auricular artery with an oil-based formulation of ceftiofur died within 5 minutes of injection. Notable pathologic findings included distention and obstruction of cerebral and cerebellar arteries by a whitish tan material and hemorrhages within meningeal spaces, the choroid plexus, cerebrum, and cerebellum. Lipid material was identified wi ...
... To compare the accuracy of a 3rd (Dolphin Voyager) versus 1st generation pulse oximeter (Nellcor N-180). Prospective laboratory investigation. Eight adult dogs. In anesthetized dogs, arterial oxygen saturation (SpO₂) was recorded simultaneously with each pulse oximeter. The oxygen fraction in inspired gas (FiO₂) was successively reduced from 1.00 to 0.09, with re-saturation (FiO₂ 0.40) after each ...
... Canadian physical activity guidelines recommend older adults accumulate 150 min of weekly moderate to vigorous physical activity (MVPA). Older adults who are insufficiently active may have reduced blood vessel health and an increased risk of cardiovascular disease. We tested this hypothesis in 11 older adults who did (7 female; age, 65 ± 5 years; MVPA, = 239 ± 81 min/week) and 10 older adults who ...
... The recent identification of acid sensing ion channels (ASICs) in vascular beds suggests their possible involvement in modulating vasomotor tone. Therefore, we investigated the gene expression profiles of ASIC subtypes in the middle cerebral artery (MCA) of Wistar rats and the functional implication of ASICs in acidosis-induced relaxation as well as maintenance of resting tension.Real time PCR was ...