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... An ultrastructural study of the vegetative thallus of Collema tenax (SW). Ach, showed that although haustorial connections were absent, the mycobiont did occasionally penetrate the algal sheath material. Concentric bodies and mesosome‐like structures were characteristically found in the mycobiont. Normal and senescent blue‐green algal cells were found alongside unusual vesiculating cells. Vesicles ...
... The brainstems of four sheep with spontaneous listeric encephalitis had scattered small foci of inflammatory cells (neutrophils or macrophages, or both) with scattered fragments of degenerating nerve fibers and glial cells. In extensive areas of malacia in the pons and medulla oblongata, there was loss of parenchyma with massive accumulation of macrophages, a few neutrophils, lymphocytes and plasm ...
... Microbial contamination of parenteral nutrition solutions is a preventable cause of infection in patients receiving nutrition support. The components of the parenteral nutrition solutions have variable microbial growth potential. Crystalline amino acid and dextrose solutions are poor growth media for bacteria but may support fungal growth. Lipid emulsions provide an excellent medium for growth of ...
... In a survey of 186 randomly selected microbial strains isolated from composted manure, 63 transformed oleic acid into three types of products: hydroxy fatty acid, fatty amide, and less polar oleyl lipid. Selection of oleic acid-transforming microorganisms was enhanced in nutrient agar supplemented with 0.1% (vol/vol) oleic acid at pH 7.2. Most of the 63 diverse isolates elicited inconsistent and p ...
... The effect of radapertization using 60Co gamma radiation on the chemical and organoleptic properties of ground and whole dry pungent red pepper has been investigated. The fungal population was eliminated with a dose of 7.5 kGy and a dose of 10 kGy was required to radapertize the samples. Irradiation up to 10 kGy did not produce any significant (P > 0.05) changes in the contents of capsaicin and ca ...
... Salmon slices (Salmo salar) were packed under CO2 and stored in a chill room at 2 + 1 degrees C for 3 weeks. A study was carried out on the effect of CO2 atmosphere on bacterial growth as well as on chemical deterioration (pH, total volatile bases, (TVB), trimethylamine (TMA), lipid oxidation) and sensory features (exudation, raw fish odour, cooked fish flavour). Results were compared with a contr ...
... The thermophilic eubacterium Bacillus stearothermophilus has been used as a model system to identify DDT-promoted events in biological membranes putatively related with the insecticide toxicity. Two strategies have been approached: a) bacterial growth and viability were followed and the effects of DDT (2,2-bis(p-chlorophenyl)-1,1,1-trichloroethane) determined; b) biophysical studies with fluoresce ...
... Juvenile channel catfish, Ictalurus punctatus (Rafinesque), blue catfish, I. furcatus (Lesueur), and their reciprocal Fl hybrids were fed practical diets containing 25% and 45% protein during a 10‐week trial to determine the effects of genotype, dietary protein level and genotype X diet interactions on growth, feed conversion ratio (FCR), fillet proximate composition and resistance to the bacteriu ...
... Extensive investigations, worldwide, in the last 4 decades have shown the benefits of radiation processing for the preservation and microbial quality improvement of seafoods. In the present review, the various factors determining the quality of seafoods are first presented. The basic principles underlying the effects of ionizing radiation and specific effects on food constituents such as proteins, ...
Shigella flexneri; bacteria; epithelial cells; lipids; pH; phagosomes; protein secretion; type III secretion system
Abstract:
... Entry of Shigella flexneri into epithelial cells and lysis of the phagosome involve the IpaB, IpaC, and IpaD proteins, which are secreted by type III secretion machinery. We report here the purification of IpaB and IpaD and the characterization of their lipidâbinding properties as a function of pH. The interaction of IpaB with the membrane was quite independent of the pH whereas that of IpaD too ...
bacteria; flow cytometry; laws and regulations; lipids; milk; milk proteins; rapid methods; raw milk
Abstract:
... Application of flow cytometry (FCM) to microbial analysis of milk is hampered by the presence of milk proteins and lipid particles. Here we report on the development of a rapid (< or = 1-h) FCM assay based on enzymatic clearing of milk to determine total bacteria in milk. When bacteria were added to ultra-heat-treated milk, a good correlation (r > or = 0.98) between the FCM assay and the more conv ...
... The quality of frozen catfish fillets was monitored over a period of 11 months at -20 degrees C, in two types of packaging materials, namely vacuum packaging (VP) and oxygen-permeable packaging (OPP). Representative samples (n = 5) from both types of packaging materials were drawn at random, monthly for the full period of the trial. Samples were pooled and analysed microbiologically using standard ...
... Effects of supplementing soy protein or casein diet with isoflavones on intestinal microflora and plasma concentrations of lipids and isoflavone metabolites were studied. Male mice were fed a soy protein or casein diet supplemented with isoflavones for four weeks, and feces and plasma samples were collected. Animals were also fed the soy protein or casein diet and feces were collected to investiga ...
... Aims: To study the biochemical response of Yarrowia lipolytica LGAM S(7)1 during growth on raw glycerol (the main by-product of bio-diesel production units) in order to produce metabolic products of industrial significance. Methods and Results: Yarrowia lipolytica was cultivated on raw glycerol or glucose in flasks. Although nitrogen-limited media were employed, growth was not followed by producti ...
... : AbstractThe complementary DNA coding for European sea bass somatolactin was expressed in the pET-3a bacteria expression vector. The recombinant somatolactin (rbSL) was purified by size exclusion chromatography, and 95% of the protein remained in the oxidized form with negligible aggregation over prolonged cold storage. The identity of the recombinant protein was demonstrated by Western blotting ...
BCG vaccine; Mycobacterium bovis BCG; Mycobacterium tuberculosis; adjuvants; auxotrophs; bacteria; bioinformatics; death; drug therapy; etiological agents; genome; growth retardation; human diseases; immune response; lipids; multiple drug resistance; phagocytes; recombinant vaccines; tuberculosis; vaccine development
Abstract:
... Tuberculosis, a bacterial disease prevalent since ancient times, continues to cause the most deaths globally compared with all other diseases. The causative agent Mycobacterium tuberculosis is responsible for different types of tuberculosis in humans; however, pulmonary tuberculosis is the most common and causes the most deaths. Mycobacterium tuberculosis is an intracellular pathogenic bacterium, ...
... The chicken meat was stored by the Hyo-on (controlled freezing point) CA (controlled atmosphere by introducing carbon dioxide, 100%) storage method. Then changes in the qualities of chicken meat stored by the Hyo-on CA storage method were monitored. Based on the measurement data thus obtained, the chicken meat qualities were evaluated depending on freshness (taste), smell, lipids, microbial prolif ...
... Some ice-nucleating bacterial strains, including Pantoea ananatis (Erwinia uredovora), Pseudomonas fluorescens, and Pseudomonas syringae isolates, were examined for the ability to shed ice nuclei into the growth medium. A novel ice-nucleating bacterium, Pseudomonas antarctica IN-74, was isolated from Ross Island, Antarctica. Cell-free ice nuclei from P. antarctica IN-74 were different from the con ...
... An extracellular protease producing bacteria Bacillus circulans (Lr 1.1) was isolated from the gut of rohu, Labeo rohita, fingerlings, and used as a supplement in five diets for rohu fingerlings. The effect of the supplement on the growth performance and feed utilization efficiency of the rohu was evaluated. Rohu fingerlings (avg wt 0.83+/-0.02 g) were fed diets supplemented with 1.5 x 10(4) (diet ...
... Iron, as the ferrous or ferric ion, is essential for the life processes of all eukaryotes and most prokaryotes; however, the element is toxic when in excess of that needed for cellular homeostasis. Ferrous ions can react with metabolically generated hydrogen peroxide to yield toxic hydroxyl radicals that in turn degrade lipids, DNA, and other cellular biomolecules. Mechanisms have evolved in livin ...
van der Meer, Marcel T. J.; Schouten, Stefan; Bateson, Mary M.; Nůbel, Ulrich; Wieland, Andrea; Kůhl, Michael; de Leeuw, Jan W.; Sinninghe Damsté, Jaap S.; Ward, David M.
... Green nonsulfur-like bacteria (GNSLB) in hot spring microbial mats are thought to be mainly photoheterotrophic, using cyanobacterial metabolites as carbon sources. However, the stable carbon isotopic composition of typical Chloroflexus and Roseiflexus lipids suggests photoautotrophic metabolism of GNSLB. One possible explanation for this apparent discrepancy might be that GNSLB fix inorganic carbo ...
Shewanella oneidensis; bacteria; lipids; proteins; temperature
Abstract:
... Shewanella oneidensis MR-1 is a mesophilic bacterium with a maximum growth temperature of [approximately]35°C but the ability to grow over a wide range of temperatures, including temperatures near zero. At room temperature ([approximately]22°C) MR-1 grows with a doubling time of about 40 min, but when moved from 22°C to 3°C, MR-1 cells display a very long lag phase of more than 100 h followed by v ...
bacteria; desorption; ionization; lasers; lipids; mass spectrometry
Abstract:
... On-probe sample pretreatment using trifluoroacetic acid as an additional reagent enabled the direct detection of phospholipids in whole bacteria by means of matrix-assisted laser desorption ionization mass spectrometry for not only gram-negative organisms but also gram-positive ones with a thicker peptidoglycan layer. ...
... To devise proper strategies to evaluate and maintain the quality of cultured blue mussels (Mytilus edulis) during storage on ice, this study examined the potential use of microbial growth, pH, lipid oxidation, and composition characteristics as quality indicators. It also evaluated the use of individual (i15:0, ai15:0, i16:0, ai16:0, i17:0, ai17:0, 17:0) and total bacterial fatty acid contents as ...
... Magnetic particles are increasingly used for various biomedical applications because they are easy to handle and separate from biological samples. In this work, a novel anchor molecule was used for targeted protein display onto magnetic nanoparticles. The magnetic bacterium Magnetospirillum magneticum AMB-1 synthesizes intracellular bacterial magnetic particles (BMPs) covered with a lipid bilayer ...
... The tear film is a complex mixture of secreted fluid, ions, proteins, glycoproteins, and lipids that lubricates and protects the ocular surface. Recently, several antimicrobial peptides have been described in the tear fluid. In this study, we describe the presence of the large secreted glycoprotein gp340 in the tear film. Western blot analysis showed that gp340 is abundant in secreted tears and in ...
... This study reports on two types of storage cells that are present in the mantle connective tissue of the deep-sea mussel Bathymodiolus azoricus. One type corresponds to the adipogranular cells, a kind of storage cell previously described in other bivalves. In these cells extensive regions of the cytoplasm are filled with glycogen deposits and these zones became strongly stained after histochemical ...
... The aim of this study was to investigate the lipolytic activity of cold-adapted Antarctic marine bacteria and, furthermore, the combined effect of some environmental factors on this enzymatic process. Strains were assayed for lipolytic activity on a basal medium amended with seven individual fatty acid esters. A significant activity was observed for 148 isolates (95·5% of the total screened). The ...
... The formation and activity of an As(III)-oxidising biofilm in a bioreactor, using pozzolana as bacterial growth support, was studied for the purpose of optimising fixed-bed bioreactors for bioremediation. After 60 days of continuous functioning with an As(III)-contaminated effluent, the active biofilm was found to be located mainly near the inflow rather than homogeneously distributed. Biofilm dev ...
... Production and characterization of biosurfactant from renewable sources. Biosurfactant production was carried out in 3-l fermentor using waste motor lubricant oil and peanut oil cake. Maximum biomass (9·8 mg ml⁻l) and biosurfactant production (6·4 mg ml⁻l) occurred with peanut oil cake at 120 and 132 h, respectively. Chemical characterization of the biosurfactant revealed that it is a glycolipopep ...
... Southern sea otters (Enhydra lutris nereis) from the California coast continue to exhibit a slower population regrowth rate than the population in Alaska. Infectious diseases have been identified as a frequent cause of death. Infectious diseases caused by varied pathogens including bacteria, fungi, and parasites were suggestive of compromised immunological health of mature animals in this populati ...
... Cold seeps in the Gulf of Mexico are often dominated by mussels of the genus Bathymodiolus that harbour symbiotic bacteria in their gills. In this study, we analysed symbiont diversity, abundance and metabolic potential in three mussel species from the northern Gulf of Mexico: Bathymodiolus heckerae from the West Florida Escarpment, Bathymodiolus brooksi from Atwater Valley and Alaminos Canyon, an ...
... Municipal wastewater treatment plants in the USA produce over 6.2 x 10⁶ t of dried sewage sludge every year. This microorganism-rich sludge is often landfilled or used as fertilizer. Recent restrictions on the use of sewage sludge, however, have resulted in increased disposal problems. Extraction of lipids from sludge yields an untapped source of cheap feedstock for biodiesel production. Solvents ...
... ATP-binding cassette (ABC) transporters are integral membrane proteins that translocate a wide variety of substrates across cellular membranes and are conserved from bacteria to humans. Here we compare four x-ray structures of the bacterial ABC lipid flippase, MsbA, trapped in different conformations, two nucleotide-bound structures and two in the absence of nucleotide. Comparison of the nucleotid ...
... Frankia alni induces root nodules on Alnus, in which the bacterium differentiates into nitrogen (N)-fixing cells called vesicles. In culture, F. alni also undergoes major morphological changes as it alternates between N-replete and N-fixing conditions. Lack of biologically available N induces the synthesis of vesicles in which nitrogenase is protected from molecular oxygen by a thick lipid hopanoi ...
Cyanobacteria; autotrophs; bacteria; bicarbonates; biomass; carbon; centrifugation; fermentation; glucose; lipids; national parks; organic compounds; spring; United States
Abstract:
... Alkaline siliceous hot spring microbial mats in Yellowstone National Park are composed of two dominant phototropic groups, cyanobacteria and green non-sulfur-like bacteria (GNSLB). While cyanobacteria are thought to cross-feed low-molecular-weight organic compounds to support photoheterotrophic metabolism in GNSLB, it is unclear how this could lead to the heavier stable carbon isotopic signatures ...
Ventura, Gregory T.; Kenig, Fabien; Reddy, Christopher M.; Schieber, Juergen; Frysinger, Glenn S.; Nelson, Robert K.; Dinel, Etienne; Gaines, Richard B.; Schaeffer, Philippe
... Highly cracked and isomerized archaeal lipids and bacterial lipids, structurally changed by thermal stress, are present in solvent extracts of 2,707- to 2,685-million-year-old (Ma) metasedimentary rocks from Timmins, ON, Canada. These lipids appear in conventional gas chromatograms as unresolved complex mixtures and include cyclic and acyclic biphytanes, C₃₆-C₃₉ derivatives of the biphytanes, and ...
... Hopanoids are ubiquitous isoprenoid lipids found in modern biota, in recent sediments and in low-maturity sedimentary rocks. Because these lipids primarily are derived from bacteria, they are used as proxies to help decipher geobiological communities. To date, much of the information about sources of hopanoids has come from surveys of culture collections, an approach that does not address the vast ...
... The flowers of Origanum sipyleum L., which is an endemic species in Turkey, are traditionally used as a beverage and medicinal tea. The extract was analyzed by reversed-phase high-performance liquid chromatography. The main phenolic compounds of O. sipyleum contained 6.81 ± 1.95, 3.94 ± 1.65, 6.78 ± 1.53, 6.71 ± 1.01, 4.95 ± 3.30, 870.22 ± 82.66, 19.47 ± 1.77 and 1017.33 ± 93.06 mg/100 g herb of a ...
... Sauces (15 and 20% brine solution) were made from African yam beans (AYBs) using a combination of solid and liquid state fermentations as practiced in Indonesia. They were analyzed to ascertain their nutritive value, microbiological safety and consumer acceptability. Results showed that the protein, fat, ash and carbohydrate contents were 4.85, 3.82, 11.82 and 16.75%, respectively, for 15% brine s ...
... Alfalfa (Medicago sativa) plants were exposed to drought to examine the involvement of carbon metabolism and oxidative stress in the decline of nitrogenase (N₂ase) activity. Exposure of plants to a moderate drought (leaf water potential of -1.3 MPa) had no effect on sucrose (Suc) synthase (SS) activity, but caused inhibition of N₂ase activity (-43%), accumulation of succinate (+36%) and Suc (+58%) ...
... Crystalline bacterial cell surface layers (S-layers) have been identified in a great number of different species of bacteria and represent an almost universal feature of archaea. Isolated native S-layer proteins and S-layer fusion proteins incorporating functional sequences self-assemble into monomolecular crystalline arrays in suspension, on a great variety of solid substrates and on various lipi ...
... The distribution of core lipids in the membranes of nine different species of the order Thermotogales, one of the early and deep branching lineages in the Bacteria, were examined by HPLC/MS and demonstrated to consist of membrane-spanning diglycerol lipids comprised of diabolic acid-derived alkyl moieties. In the Thermotoga species the core membrane lipids are characterized by the presence of both ...
... Dairy products are suitable vehicles for delivering beneficial microorganisms to consumers. Both Lactobacillus reuteri RC-14 and Lactobacillus rhamnosus GR-1 are considered as probiotic agents with therapeutic properties. The objective of this study was to monitor growth and survival of these bacteria in milk during storage period. Four formulations of milk (1% fat) with 0.33% yeast extract (Y), 0 ...