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... 1 The present contribution, as part of a general study of pink rot of the potato, deals with Phytophthora erythroseptica Pethybr. in its relation to staling, light, acidity, moisture and temperature. 2 In natural media the fungus is not, or but very slightly, affected by its own staling products, but, in general, is intensely intolerant to the presence of other fungi and bacteria. 3 Ordinary dayli ...
... Plants may be useful in stabilization and remediation of polluted surface soils, but phytotoxicity and plant bioconcentration of some pollutants can interfere with plant-associated site remediation. Pseudomonas strain SR3, when applied to seeds of proso millet (Panicum miliaceum L.), protected the growing plants from phytotoxicity of pentachlorophenol (PCP) in the soil during a 4-wk growth-chamber ...
... Anaerobic bacteria that reduce hexavalent chromium [Cr(VI)] to trivalent [Cr(III)] are common in soils and were used to develop a bioprocess employing a selection strategy. Indigenous Cr(VI)-reducers were enriched from Cr(VI)contaminated soil under anaerobic conditions. The mixed culture was then tested for Cr(VI)-reducing activity in a chemostat, followed by transfer to a 1-L packed-bed bioreacto ...
... The use of an indigenous microbial consortium, pollutant-acclimated and attached to soil particles (activated soil), was studied as a bioaugmentation method for the aerobic biodegradation of pentachlorophenol (PCP) in a contaminated soil. A 125-1 completely mixed soil slurry (10% soil) bioreactor was used to produce the activated soil biomass. Results showed that the bioreactor was very effective ...
... 4-Nitrophenol degrading bacterial strainCorynebacterium sp. 8/3 was isolated from chemically polluted soil. The product of cometabolic transformation of 4-nitrophenol was identified as 4-nitrocatechol., Effect of immobilization (encapsulation in calcium alginate) ofCorynebacterium sp. cells on the process of 4-nitrophenol transformation was investigated. 4-Nitrophenol was converted by encapsulated ...
... The possibility of enhancing the ex situ bioremediation of a chronically polychlorinated biphenyl (PCB)-contaminated soil by using Triton X-100 or Quillaya Saponin, a synthetic and a biogenic surfactant, respectively, was studied. The soil, which contained about 350 mg/kg of PCBs and indigenous aerobic bacteria capable of growing on biphenyl or on monochlorobenzoic acids, was amended with inorgani ...
... To test how a dysfunctioning ecosystem of a severely metal-polluted soil responds to renewed plant growth, a pot experiment was conducted with soil from an experimental arable field with pH and copper gradients imposed 13 years ago. In this experiment, four pH/copper combinations from this field were either planted with a pH- and copper-resistant grass cultivar or remained fallow. During a 10-week ...
... Oil spills occur in the Antarctic when fuel oils such as JP8 jet fuel are moved or stored. Hydrocarbons, both n-alkanes and aromatic compounds, have been detected in oil-contaminated soils of the Ross Dependency. In such areas hydrocarbon-degrading microbes, if naturally occurring, could be used for clean-up. Soil samples from oil-impacted and control sites were analysed for hydrocarbon-degrading ...
Pseudomonas; atrazine; bacteria; biomass; mineralization; polluted soils; slurries; soil inoculation; soil water content; starvation; water holding capacity
Abstract:
... The evaluation of pesticide-mineralising microorganisms to clean-up contaminated soils was studied with the widely applied and easily detectable compound atrazine, which is rapidly mineralised by several microorganisms including the Pseudomonas sp. strain Yaya 6. The rate of atrazine removal was proportional to the water content of the soil and the amount of bacteria added to the soil. In soil slu ...
... Bioaugmentation has previously been unreliable for the in situ clean-up of contaminated soils because of problems with poor survival and the rapid decline in activity of the bacterial inoculum. In an attempt to solve these problem, a 500-l batch fermenter was investigated for its ability to deliver inoculum repeatedly to contaminated soils via irrigation lines. In a field experiment, mesocosms wer ...
... Historical emissions of old nonferrous factories lead to large geographical areas of metals-contaminated sites. At least 50 sites in Europe are contaminated with metals like Zn, Cd, Cu, and Pb. Several methods, based on granular differentiation, were developed to reduce the metals content. However, the obtained cleaned soil is just sand. Methods based on chemical leaching or extraction or on elect ...
... A bacterial strain capable of utilizing 2-methylphenanthrene (2-MP) as its sole source of carbon and energy for growth was isolated from creosote contaminated soil. The isolate was identified as a strain of Sphingomonas sp. and was designated strain JS5. Utilization of 2-MP by strain JS5 was demonstrated by an increase in bacterial biomass concomitant with a decrease of 2-MP in liquid mineral medi ...
... An old PAH/creosote contaminated soil (total approximately 300 microgram PAH/g soil) from a former gas work site in Stockholm, Sweden, has been treated at 20 degrees C with the addition of various nutrients and inoculated with bacteria (isolated from the soil) to enhance the degradation of selected hydrocarbons. Microcosm studies showed that the soil consisted of two contaminant fractions: one ava ...
... This study investigated the biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons (PAHs) in liquid media and soil by bacteria (Stenotrophomonas maltophilia VUN 10,010 and bacterial consortium VUN 10,009) and a fungus (Penicillium janthinellum VUO 10,201) that were isolated from separate creosote- and manufactured-gas plant-contaminated soils. The bacteria could use pyrene as the ...
... Benzene, toluene, xylenes, phenol, naphthalene, and biphenyl are among a group of compounds that have at least one reported pathway for biodegradation involving catechol 2,3-dioxygenase enzymes. Thus, detection of the corresponding catechol 2,3-dioxygenase genes can serve as a basis for identifying and quantifying bacteria that have these catabolic abilities. Primers that can successfully amplify ...
... The biotreatability of a xenobiotic contaminated soil is frequently determined through a bioslurry treatment usually performed in lab-scale shaken baffled flasks. In this study, a 3-l unconventional stirred tank reactor was developed and tested in the slurry-phase treatment of a soil heavily contaminated by polychlorobiphenyls (PCBs) derived from an Italian dump site, in the absence and in the pre ...
Archaea; agricultural soils; agroecosystems; bacteria; community structure; crop rotation; denaturing gradient gel electrophoresis; microbial communities; polluted soils; polycyclic aromatic hydrocarbons; polymerase chain reaction; ribosomal DNA; ribosomal RNA; soil ecology; soil sampling; tillage; Norway; United States
Abstract:
... Separation of polymerase chain reaction (PCR)–amplified 16S rDNA products using denaturing gradient gel electrophoresis (DGGE) was tested as a means to study microbial community composition in bulk soil samples. DNA was extracted from six soils from agroecosystems in Norway and the USA under different agronomic treatments (crop, rotation, and tillage); one soil is contaminated with polyaromatic hy ...