U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Subject term bacteria Remove constraint Subject term: bacteria Subject term tissues Remove constraint Subject term: tissues
Start Over

Search

977  Search Results

« Previous | 1 - 50 of 977 |
Number of results to display per page

Search Results

1. THE Relation of the cabbage maggot, hylemyia brassicae (bouché), to decay in some cruciferous crops¹

Author:
DOANE, J.F.; CHAPMAN, R.K.
Source:
Entomologia experimentalis et applicata 1964 v.7 no.1 pp. 1-8
ISSN:
0013-8703
Subject:
Brassica napus; Brassica oleracea; Brassica rapa subsp. pekinensis; Chinese cabbage; Delia radicum; Enterobacter; Erwinia; Pseudomonas; adults; bacteria; bacterial contamination; eclosion; eggs; larvae; pigmentation; prepupae; puparium; rutabagas; tissues
Abstract:
... Bacteria pathogenic to crucifers were isolated from 92% of the eggs from flies of the cabbage maggot, Hylemyia brassicae (Bouché) of a population from rutabaga (Swede; Brassica napus napobrassicd) and from 90% of those from a population from Chinese cabbage (Brassica pekinensis). The larvae transmitted the decay-causing bacteria to healthy tissues and aided in the development and spread of the rot ...
DOI:
10.1111/j.1570-7458.1964.tb00721.x

2. Alteration of response of bean leaves to compatible and incompatible bacteria

Author:
Lyon, F.; Wood, R.K.S.
Source:
Annals of botany 1977 v.41 no.172 pp. 359-367
ISSN:
0305-7364
Subject:
Pseudomonas; bacteria; beans; chloroform; cycloheximide; electrolytes; hypersensitive response; leaves; peroxidase; tissues; tobacco; vapors
Abstract:
... Injection of Red Mexican bean leaves with Pseudomonas phascolicola Race 2 (compatible, 18 h before P. mors-prunorum or P. phaseolicola Race 1 (incompatible), or simultaneous inoculation with compatible and incompatible bacteria (3:1) greatly delayed the appearance of hypersensitive responses. When compatible bacteria were inoculated 12 h or less before incompatible bacteria, or when the ratio of t ...
DOI:
10.1093/oxfordjournals.aob.a085298

3. Nontuberculous Mycobacterial Disease In Rhesus Monkeys

Author:
C.A. Holmberg; R.V. Henrickson; C. Malaga; R. Schneider; D. Gribble
Source:
Veterinary pathology v.19 no.7_suppl pp. 9-16
ISSN:
1544-2217
Subject:
Macaca mulatta; Mycobacterium avium complex; animal pathology; bacteria; colitis; diarrhea; large intestine; lungs; lymph nodes; macrophages; monkeys; mycobacterial diseases; serotypes; small intestine; tissues; tuberculin; weight loss
Abstract:
... In a colony of rhesus monkeys (Macaca mulatta), 42 cases of nontuberculous mycobacterial-related disease were identified from 1970 to 1978. The disease affected young and old colony-born and wild-caught monkeys of both sexes. Serotypes 1, 2, 4, 8, and 18 of the Mycobacterium avium-intracellulare group were isolated from different monkeys. The lesions were primarily intestinal in 36 monkeys. Lesion ...
DOI:
10.1177/030098588201907s02

4. Pseudomonas syringae pv. helianthi (Kawamura 1934) Dye, Wilkie et Young 1978, a Pathogen of Sunflower (Helianthus annuus L.)

Author:
M. Arsenijević; R. J. Vennete; S. Maširević
Source:
Phytopathologische Zeitschrift 1994 v.142 no.4 pp. 199-208
ISSN:
0931-1785
Subject:
Helianthus annuus; Pseudomonas syringae; bacteria; fluorescence; host range; leaves; pathogenicity; pathogens; strains; testing; tissues
Abstract:
... Among the bacterial strains isolated from diseased sunflower leaves, eight were studied in some detail. A fluorescent pseudomonad isolated from necrotic tissues and its reisolates belong to group Ia of phytopathogenic pseudomonads which includes Pseudomonas syringae bacterium. A study of host range indicated that the pathogen infects only sunflower but not the other plant species. Based on the pat ...
DOI:
10.1111/j.1439-0434.1994.tb00013.x

5. Biological control ofBotrytis cinerea in residues and flowers of Rose (Rosa hybrida)

Author:
Joseli da Silva Tatagiba; Luiz Antonio Maffia; Roberto W. Barreto; Acelino C. Alfenas; John C. Sutton
Source:
Phytoparasitica 1998 v.26 no.1 pp. 8-19
ISSN:
0334-2123
Subject:
Bacillus subtilis; Botrytis cinerea; Cladosporium cladosporioides; Clonostachys rosea; Rosa; Trichoderma harzianum; antagonists; bacteria; beneficial microorganisms; biological control; buds; corolla; hybrids; inoculum; leaves; pathogens; sporulation; tissues; vinclozolin; yeasts
Abstract:
... Microbial isolates from living petals, petal residues and leaf residues of rose, and from laboratory collections, were evaluated for control ofBotrytis cinerea in rose. In leaf residues artificially infested withB. cinerea, isolates of the filamentous fungiGliocladium roseum, FR136 (unidentified) andTrichoderma inhamatum reduced sporulation of the pathogen by >90%, other filamentous fungi were 25– ...
DOI:
10.1007/BF02981261

6. Rat liver contains age-regulated cytosolic 3-deoxy-d-glycero-d-galacto-non-2ulopyranosonic acid (Kdn)

Author:
M.Asunción Campanero-Rhodes; Dolores Solís; Elena Carrera; M.Julia de la Cruz; Teresa Díaz-Mauriño
Source:
Glycobiology 1999 v.9 no.6 pp. 527-532
ISSN:
1460-2423
Subject:
bacteria; derivatization; elderly; glycoconjugates; liver; neonates; postnatal development; rats; reversed-phase high performance liquid chromatography; sialic acid; subcellular fractions; tissues
Abstract:
... Kdn (3-deoxy-d-glycero-d-galacto-non-2-ulopyranosonic acid), a unique deaminated member of the sialic acid family, has emerged as a new building block of glycoconjugates from a wide variety of organisms, ranging from bacteria to mammals. In particular, the presence of Kdn has been demonstrated in different rat organs and tissues, but not in liver. Here we report on the detection and quantitation o ...
DOI:
10.1093/glycob/9.6.527

7. Serological diagnosis of ratoon stunting disease of sugarcane in india

Author:
G. P. Rao; G. P. Singh
Source:
Sugar tech 2000 v.2 no.4 pp. 35-36
ISSN:
0972-1525
Subject:
antigens; antiserum; bacteria; enzyme-linked immunosorbent assay; ratoon stunting disease; serodiagnosis; sugarcane; tissues; India
Abstract:
... Serological observations of stunted sugarcane plants of predominant varieties in India indicated the occurrence of ratoon stunting disease. The incidence ranged from 3 to 11 per cent on infected clump basis in different varieties. In the serological observations, diffusates from the infected stalks/tissues were used as antigen for identification of bacteriumClavibacter xyli subsp. xyli by DAC — EL ...
DOI:
10.1007/BF02995585

8. Temperature-regulated protein synthesis by Leptospira interrogans

Author:
Nally, J.E.; Timoney, J.F.; Stevenson, B.
Source:
Infection and immunity 2001 v.69 no.1 pp. 400-404
ISSN:
0019-9567
Subject:
Leptospira interrogans; antibodies; bacteria; cytoplasm; environmental factors; fractionation; horses; host-pathogen relationships; immunoblotting; lipoproteins; outer membrane proteins; pathogens; protein synthesis; serotypes; temperature; thermoregulation; tissues; virulent strains
Abstract:
... Leptospira interrogans is an important mammalian pathogen. Transmission from an environmental source requires adaptations to a range of new environmental conditions in the organs and tissues of the infected host. Since many pathogenic bacteria utilize temperature to discern their environment and regulate the synthesis of appropriate proteins, we investigated the effects of temperature on protein s ...
DOI:
10.1128/IAI.69.1.400-404.2001
PubMed:
11119530
PubMed Central:
PMC97896

9. Bacteria in heart and lungs of young chicks

Author:
Tankson, J.D.; Thaxton, J.P.; Vizzier-Thaxton, Y.
Source:
Journal of applied microbiology 2002 v.92 no.3 pp. 443-450
ISSN:
1364-5072
Subject:
rearing; bacteria; heart; air; lungs; embryogenesis; chicks; tissues
Abstract:
... Aims: The purpose of these experiments was to determine whether the heart and lungs of young chicks harboured bacteria. Methods and Results: Samples of the heart and lungs were aseptically removed from chicks on scheduled sampling days. Experiment 1 showed that of the 360 birds evaluated during the late embryonic and early post-hatching periods, only 10.8% harboured bacteria in the heart, lungs, a ...
DOI:
10.1046/j.1365-2672.2002.01546.x
PubMed:
11872119

10. Expression and distribution of penaeidin antimicrobial peptides are regulated by haemocyte reactions in microbial challenged shrimp

Author:
Marcelo Muñoz; Franck Vandenbulcke; Denis Saulnier; Evelyne Bachère
Source:
European journal of biochemistry 2002 v.269 no.11 pp. 2678-2689
ISSN:
0014-2956
Subject:
Penaeidae; antimicrobial peptides; bacteria; blood circulation; hemocytes; immune response; messenger RNA; shrimp; tissues
Abstract:
... Penaeidins are a family of antimicrobial peptides constitutively produced and stored in the haemocytes of penaeid shrimp. In response to microbial stimulation, they are released into the blood circulation and they further attach to shrimp cuticle surfaces through a chitin‐binding property. In the present paper, we have analysed their expression, regulation and distribution in shrimp tissues in r ...
DOI:
10.1046/j.1432-1033.2002.02934.x

11. Learning Vector Quantization for Color Classification of Diseased Air Sacs in Chicken Carcasses

Author:
J. G. Ibarra; Y. Tao; L. Newberry; Y. R. Chen
Source:
TRANSACTIONS OF THE ASAE ONLINE 2002 v.45 no.5 pp. 1629-1635
ISSN:
0001-2351
Subject:
Escherichia coli; USDA; air sacs; algorithms; bacteria; chicken carcasses; color; food quality; image analysis; meat processing plants; neural networks; poultry; tissues; United States
Abstract:
... The variation in color features observed during the evolution of air-sacculitis in chicken carcasses is exploited to classify the disease using digital imaging and neural networks. For the experiments, air-sacculitis was induced by secondary infected of E. coli via direct inoculation of challenge bacteria. Mild and severely infected samples were obtained and imaged. For the supervised classificati ...
DOI:
10.13031/2013.11048

12. Macrophage plasma membrane cholesterol contributes to Brucella abortus infection of mice

Author:
Watarai, M.; Makino, S.; Michikawa, M.; Yanagisawa, K.; Murakami, S.; Shirahata, T.
Source:
Infection and immunity 2002 v.70 no.9 pp. 4818-4825
ISSN:
0019-9567
Subject:
Brucella melitensis biovar Abortus; bacteria; bacterial infections; cholesterol; enzyme inhibitors; genes; ketoconazole; low density lipoprotein; lysosomes; macrophages; mice; plasma membrane; tissues
Abstract:
... Brucella abortus is a facultative intracellular bacterium capable of surviving inside macrophages. Intracellular replication of B. abortus requires the VirB complex, which is highly similar to conjugative DNA transfer systems. In this study, we show that plasma membrane cholesterol of macrophages is required for the VirB-dependent internalization of B. abortus and also contributes to the establish ...
DOI:
10.1128/IAI.70.9.4818-4825.2002
PubMed:
12183525
PubMed Central:
PMC128274

13. Pathogenicity, Transmissibility, and Tissue Distribution of Avian Pneumovirus in Turkey Poults

Author:
Alkhalaf, A.N.; Ward, L.A.; Dearth, R.N.; Saif, Y.M.
Source:
Avian diseases 2002 v.46 no.3 pp. 650-659
ISSN:
0005-2086
Subject:
Avian metapneumovirus; Bordetella avium; RNA; air flow; antibodies; bacteria; bird diseases; cages; direct contact; indirect contact; lungs; pathogenicity; poults; reverse transcriptase polymerase chain reaction; sinuses; tissue distribution; tissues; turkeys; viruses
Abstract:
... The pathogenicity, transmissibility, tissue distribution, and persistence of avian pneumovirus (APV) in turkey poults were investigated in three experiments. In the first experiment, we inoculated 2-wk-old commercial turkey poults oculonasally with APV alone or in combination with Bordetella avium. In the dually infected group, clinical signs were more severe, the virus persisted longer, the bacte ...
DOI:
10.1637/0005-2086(2002)046[0650:PTATDO]2.0.CO;2

14. Rainforest Endophytes and Bioactive Products

Author:
Gary A. Strobel
Source:
Critical reviews in biotechnology 2002 v.22 no.4 pp. 315-333
ISSN:
1549-7801
Subject:
Protozoa; animal health; bacteria; bioactive compounds; bioassays; culture filtrates; drug resistance; endophytes; fractionation; fungi; grasses; harvesting; host-pathogen relationships; human health; neoplasms; paclitaxel; rain forests; spectroscopy; tissues
Abstract:
... An increase in the number of people in the world having health problems caused by certain cancers, drug-resistant bacteria, parasitic protozoans, and fungi has caused alarm. An intensive search for newer and more effective agents to deal with these problems is now underway. Endophytes are a potential source of novel chemistry and biology to assist in helping solve not only human health, but plant ...
DOI:
10.1080/07388550290789531
PubMed:
12487423

15. Streptococcus suis interactions with the murine macrophage cell line J774: adhesion and cytotoxicity

Author:
Segura, M.; Gottschalk, M.
Source:
Infection and immunity 2002 v.70 no.8 pp. 4312-4322
ISSN:
0019-9567
Subject:
Streptococcus suis; adhesion; bacteremia; bacteria; bacterial adhesion; blood flow; blood serum; cell lines; cholesterol; complement; cytotoxicity; enzyme-linked immunosorbent assay; etiological agents; inflammation; macrophages; meninges; meningitis; mice; moieties; pathogenesis; sialic acid; swine; tissues; transmission electron microscopy
Abstract:
... Streptococcus suis capsular type 2 is an important etiological agent of swine meningitis, and it is also a zoonotic agent. Since one hypothesis of the pathogenesis of S. suis infection is that bacteria enter the bloodstream and invade the meninges and other tissues in close association with mononuclear phagocytes, the objective of the present study was to evaluate the capacity of S. suis type 2 to ...
DOI:
10.1128/IAI.70.8.4312-4322.2002
PubMed:
12117940
PubMed Central:
PMC128179

16. Developing disease-suppressive soils through crop rotation and tillage management practices

Author:
Peters, R.D.; Sturz, A.V.; Carter, M.R.; Sanderson, J.B.
Source:
Soil & tillage research 2003 v.72 no.2 pp. 181-192
ISSN:
0167-1987
Subject:
minimum tillage; tubers; potatoes; silver scurf; endophytes; spring barley; rhizosphere; Thanatephorus cucumeris; Phytophthora erythroseptica; tissues; pathogens; agroecosystems; Helminthosporium solani; disease control; soil; Trifolium pratense; crop rotation; Fusarium; antibiosis; greenhouse experimentation; soil-borne diseases; bacteria; Prince Edward Island
Abstract:
... A field laboratory was established in Prince Edward Island, Canada, to determine the effects of 2- and 3-year crop rotations, with conventional and minimum tillage treatments, on the severity of soilborne diseases of potato. The 2-year rotation consisted of spring barley and potato (cv. ‘Russet Burbank’), and the 3-year rotation was barley (undersown with red clover), red clover and potato. Examin ...
DOI:
10.1016/S0167-1987(03)00087-4

17. Homalodisca coagulata (Hemiptera, Cicadellidae) Transmission of Xylella fastidiosa to Almond

Author:
Almeida, R.P.P.; Purcell, A.H.
Source:
Plant disease 2003 v.87 no.10 pp. 1255-1259
ISSN:
0191-2917
Subject:
stone fruits; grapes; epidemiology; Cercopidae; plant pathology; Xylella fastidiosa; trees; almond leaf scorch; Homalodisca vitripennis; petioles; almonds; population; strains; insects; tissues; bacteria; shoots; California
Abstract:
... Almond leaf scorch (ALS) is caused by the bacterium Xylella fastidiosa, transmitted by sharpshooter leafhoppers and spittlebugs. The recent invasion of a X. fastidiosa vector, Homalodisca coagulata (Hemiptera, Cicadellidae), into California may have major consequences to the spread of ALS because this insect feeds readily on trees, including stone fruit species. We found that, under laboratory con ...
DOI:
10.1094/PDIS.2003.87.10.1255

18. Immunohistochemical Localization of Clostridium perfringens β2-Toxin in the Gastrointestinal Tract of Horses

Author:
L. N. Bacciarini; P. Boerlin; R. Straub; J. Frey; A. Gröne
Source:
Veterinary pathology v.40 no.4 pp. 376-381
ISSN:
1544-2217
Subject:
Clostridium perfringens A; animal pathology; antitoxins; bacteria; diagnostic techniques; digestive system diseases; horses; immunohistochemistry; large intestine; polyclonal antibodies; polymerase chain reaction; rabbits; retrospective studies; small intestine; stomach; tissues
Abstract:
... Clostridia-associated intestinal disease in horses was generally reported to be due to infection with Clostridium perfringens type A, which harbors the cpa-encoded α-toxin. A recent study demonstrated a high incidence of β2-toxigenic C. perfringens in horses suffering or dying from typhlocolitis, suggesting that this novel type of C. perfringens might play an important role in typhlocolitis and po ...
DOI:
10.1354/vp.40-4-376

19. New Considerations for Pruning in Management of Fire Blight in Pears

Author:
Shtienberg, D.; Zilberstaine, M.; Oppenheim, D.; Levi, S.; Shwartz, H.; Kritzman, G.
Source:
Plant disease 2003 v.87 no.9 pp. 1083-1088
ISSN:
0191-2917
Subject:
Erwinia amylovora; bacteria; branches; cutting; growers; host plants; pathogens; pears; physiological state; plant pathology; pruning; spring; tissues; trees; Israel
Abstract:
... The efficacy of pruning infected pear tissues to combat fire blight (caused by Erwinia amylovora) was evaluated in two sets of experiments conducted during 1999 to 2001 in Israel. In the first set of two experiments, diseased tissues were removed soon after the observation of blossom infections. Pruning was effective in 0 to 50% of the treated trees, and resulted in complete eradication of E. amyl ...
DOI:
10.1094/PDIS.2003.87.9.1083

20. Pharmacokinetic–pharmacodynamic integration of danofloxacin in the calf

Author:
Aliabadi, F. Shojaee; Lees, P.
Source:
Research in veterinary science 2003 v.74 no.3 pp. 247-259
ISSN:
0034-5288
Subject:
Mannheimia haemolytica; antibacterial properties; bacteria; blood serum; calves; clinical trials; cross-over studies; danofloxacin; drugs; half life; intravenous injection; minimum inhibitory concentration; pathogens; pharmacokinetics; plate count; tissues
Abstract:
... The pharmacokinetics and pharmacodynamics of danofloxacin were studied in calves after intravenous (IV) and intramuscular (IM) administration, at a dose of 1.25 mg/kg in a two period cross-over study, using tissue cages to monitor aspects of extravascular distribution. Danofloxacin had a high volume of distribution (3.90 L/kg) and relatively rapid clearance (1.02 L/kg h) after IV dosing. Terminal ...
DOI:
10.1016/S0034-5288(03)00005-5

21. Suppression of Meloidogyne javanica by Pseudomonas aeruginosa IE-6S+ in tomato: the influence of NaCl, oxygen and iron levels

Author:
Siddiqui, Imran Ali; Shaukat, Syed Shahid; Khan, Ghazala Habib; Ali, Nasima Imam
Source:
Soil biology & biochemistry 2003 v.35 no.12 pp. 1625-1634
ISSN:
0038-0717
Subject:
Meloidogyne javanica; Pseudomonas aeruginosa; Pseudomonas fluorescens; bacteria; biological control; culture filtrates; environmental factors; greenhouses; hydrogen cyanide; iron; juveniles; leaf protein; leaves; mortality; nematicidal properties; oxygen; plant growth; protein content; rhizosphere; root-knot nematodes; secondary metabolites; seedlings; shoots; sodium chloride; soil; soil amendments; tissues; tomatoes
Abstract:
... Understanding the environmental factors that influence the suppression of disease-suppressive strains of Pseudomonas fluorescens is an essential step toward improving the level and reliability of their biocontrol activity. A 0.8 M NaCl concentration was optimal for in vitro survival and growth of IE-6S(+)while, nematicidal activity by IE-6S(+) was maximal when the bacterium was exposed to 0.4 M Na ...
DOI:
10.1016/j.soilbio.2003.08.007

22. Enhancing the frequency of somatic embryogenesis following agrobacterium-mediated transformation of immature cotyledons of soybean \[glycine max (l.) merrill.\]

Author:
Ko Tae-Seok; Korban Schuyler S.
Source:
In vitro cellular & developmental biology 2004 v.40 no.6 pp. 552-558
ISSN:
1054-5476
Subject:
Agrobacterium; Glycine max; acetosyringone; bacteria; coculture; cotyledons; explants; genetically modified organisms; kanamycin; phenotype; somatic embryogenesis; somatic embryos; soybeans; tissue culture; tissues
Abstract:
... A characteristic phenotype of highly embryogenic explants along with the location of embryogenesis- and transformation-competent cells/tissues on immature cotyledons of soybean \[Glycine max (L.) Merrill.\] under hygromycin selection was identified. This highly embryogenic immature cotyledon was characterized with emergence of somatic embryos and incidence of browning/necrotic tissues along the ma ...
DOI:
10.1079/IVP2004566

23. Effects of gamma radiation on Brugia malayi infective larvae and their intracellular Wolbachia bacteria

Author:
R. Rao; H. Moussa; R. P. Vanderwaal; E. Sampson; L. J. Atkinson; G. J. Weil
Source:
Parasitology research 2005 v.97 no.3 pp. 219-227
ISSN:
0932-0113
Subject:
Brugia malayi; DNA; Meriones; Wolbachia; animals; antibodies; bacteria; cesium; dose response; electron microscopy; filariasis; gamma radiation; immunity; in vitro culture; in vivo studies; irradiation; larvae; molting; quantitative polymerase chain reaction; reproduction; surface proteins; tissues
Abstract:
... Prior studies have shown that irradiated filarial larvae are developmentally stunted but capable of inducing partial immunity to filariasis in animals. The mechanisms for these effects are poorly understood. Recent studies suggest that intracellular Wolbachia bacteria are necessary for the normal development, reproduction and survival of filarial nematodes. The purpose of this study was to examine ...
DOI:
10.1007/s00436-005-1421-8

24. Human and murine paraoxonase 1 are host modulators of Pseudomonas aeruginosa quorum-sensing

Author:
Ozer, Egon A.; Pezzulo, Alejandro; Shih, Diana M.; Chun, Carlene; Furlong, Clement; Lusis, Aldons J.; Greenberg, Everett P.; Zabner, Joseph
Source:
FEMS microbiology letters 2005 v.253 no.1 pp. 29-37
ISSN:
0378-1097
Subject:
Pseudomonas aeruginosa; bacteria; biofilm; genes; humans; immunity; in vitro studies; messenger RNA; mice; models; quorum sensing; sepsis (infection); tissues; virulence
Abstract:
... The pathogenic bacterium Pseudomonas aeruginosa uses acyl-HSL quorum-sensing signals to regulate genes controlling virulence and biofilm formation. We found that paraoxonase 1 (PON1), a mammalian lactonase with an unknown natural substrate, hydrolyzed the P. aeruginosa acyl-HSL 3OC12-HSL. In in vitro assays, mouse serum-PON1 was required and sufficient to degrade 3OC12-HSL. Furthermore, PON2 and P ...
DOI:
10.1016/j.femsle.2005.09.023

25. Betaine aldehyde dehydrogenase from Pseudomonas aeruginosa: cloning, over-expression in Escherichia coli, and regulation by choline and salt

Author:
Velasco-García, Roberto; Villalobos, Miguel Angel; Ramírez-Romero, Miguel A.; Mújica-Jiménez, Carlos; Iturriaga, Gabriel; Muñoz-Clares, Rosario A.
Source:
Archives of microbiology 2006 v.185 no.1 pp. 14-22
ISSN:
0302-8933
Subject:
Escherichia coli; Pseudomonas aeruginosa; Western blotting; amino acid sequences; bacteria; betaine; betaine-aldehyde dehydrogenase; carbon; choline; gel chromatography; gene expression; glucose; humans; nitrogen; operon; osmotic stress; osmotolerance; pathogens; promoter regions; recombinant proteins; response elements; salt tolerance; sodium chloride; spectroscopy; tissues; transcription (genetics); transcription factors
Abstract:
... In the human pathogen Pseudomonas aeruginosa, betaine aldehyde dehydrogenase (BADH) may play a dual role assimilating carbon and nitrogen from choline or choline precursors--abundant at infection sites--and producing glycine betaine, which protects the bacteria against the high-osmolarity stress prevalent in the infected tissues. We cloned the P. aeruginosa BADH gene and expressed the BADH protein ...
DOI:
10.1007/s00203-005-0054-8
PubMed:
16315011

26. HvMCB1, a R1MYB transcription factor from barley with antagonistic regulatory functions during seed development and germination

Author:
Ignacio Rubio‐Somoza; Manuel Martinez; Isabel Diaz; Pilar Carbonero
Source:
plant journal 2006 v.45 no.1 pp. 17-30
ISSN:
0960-7412
Subject:
Northern blotting; aleurone cells; amylases; bacteria; barley; endosperm; genes; germination; gibberellins; in situ hybridization; messenger RNA; oligonucleotides; promoter regions; repressor proteins; seed development; tissues; transcription (genetics)
Abstract:
... The functional analysis of hydrolase gene promoters induced by gibberellin (GA) in barley aleurone cells upon germination has identified a tripartite GA‐response complex (GARC) containing a 5′‐TATCCAC‐3′ box as well as the GA‐responsive element (GARE) recognized by GAMYB and the pyrimidine box interacting with the DOF transcription factors BPBF and SAD. We show here that the MCB1 gene encoding a R ...
DOI:
10.1111/j.1365-313X.2005.02596.x

27. Influence of Limnological Conditions on Clostridium Botulinum Type E Presence in Eastern Lake Erie Sediments (Great Lakes, USA)

Author:
Alicia Pérez-Fuentetaja; Mark D. Clapsadl; Donald Einhouse; Paul R. Bowser; Rodman G. Getchell; W. Theodore Lee
Source:
Hydrobiologia 2006 v.563 no.1 pp. 189-200
ISSN:
0018-8158
Subject:
Chironomidae; Clostridium botulinum E; DNA; Gammarus; Oligochaeta; bacteria; birds; botulism; dissolved oxygen; environmental factors; fish; genes; lakes; mussels; pH; principal component analysis; quantitative polymerase chain reaction; redox potential; sampling; sediments; spores; tissues; Lake Erie; New York
Abstract:
... Avian and fish botulism outbreaks have been recorded since 1999 in eastern Lake Erie. These outbreaks are caused by Clostridium botulinum type E, a toxin-producing bacteria that is found in anoxic substrates rich in organic material. We studied the environmental conditions present in eastern Lake Erie during 2002, a year when several botulism outbreaks were observed. We also tested for the presenc ...
DOI:
10.1007/s10750-005-0011-1

28. Molecular Identification, Polymorphism, and Expression Analysis of Major Histocompatibility Complex Class IIA and B Genes of Turbot (Scophthalmus maximus)

Author:
Yu-Xi Zhang; Song-Lin Chen
Source:
Marine biotechnology 2006 v.8 no.6 pp. 611-623
ISSN:
1436-2228
Subject:
Cichlidae; Danio rerio; Ginglymostoma cirratum; Oncorhynchus mykiss; Pagrus major; Paralichthys olivaceus; Salmo salar; Scophthalmus maximus; T-lymphocytes; Vibrio anguillarum; adaptive immunity; alleles; amino acid sequences; bacteria; bass; carp; complementary DNA; flounder; humans; kidneys; liver; loci; major histocompatibility complex; messenger RNA; mice; nucleotide sequences; peptides; rapid amplification of cDNA ends; reverse transcriptase polymerase chain reaction; spleen; tissues; turbot
Abstract:
... Major histocompatibility complex (MHC) class II has a central role in the adaptive immune system by presenting foreign peptides to the T-cell receptor. The full lengths of MHC class II A and B cDNA were cloned from turbot by homology cloning and rapid amplification of cDNA ends polymerase chain reaction (RACE PCR), and genomic organization, molecular polymorphism, and expression of turbot class II ...
DOI:
10.1007/s10126-005-6174-y

29. Colonization of Female Watermelon Blossoms by Acidovorax avenae ssp. citrulli and the Relationship between Blossom Inoculum Dosage and Seed Infestation

Author:
Lessl, J.T.; Fessehaie, A.; Walcott, R.R.
Source:
Phytopathologische Zeitschrift 2007 v.155 no.2 pp. 114-121
ISSN:
0931-1785
Subject:
Acidovorax avenae subsp. citrulli; Cucurbitaceae; Pseudomonas syringae; bacteria; epiphytes; females; fruits; greenhouse production; inoculum; microbial growth; pollination; polymerase chain reaction; seedlings; stigma; tissues; tomatoes; watermelons
Abstract:
... The aim of this work was to investigate the ability of Acidovorax avenae ssp. citrulli, the causal agent of bacterial fruit blotch of cucurbits (BFB), to colonize female watermelon blossoms, and to explore the relationship between blossom inoculum dosage and seed infestation. Under greenhouse conditions A. avenae ssp. citrulli colonized stigmas and styles of female watermelon blossoms reaching pop ...
DOI:
10.1111/j.1439-0434.2007.01204.x

30. Detection of the bacterium Flavobacterium psychrophilum from a natural infection in rainbow trout, Oncorhynchus mykiss (Walbaum), using formalin-fixed, wax-embedded fish tissues

Author:
Crumlish, M.; Diab, A.M.; George, S.; Ferguson, H.W.
Source:
Journal of fish diseases 2007 v.30 no.1 pp. 37-41
ISSN:
0140-7775
Subject:
Flavobacterium psychrophilum; Oncorhynchus mykiss; bacteria; bacterial cold-water disease; bacteriology; fish; heart; histopathology; immunohistochemistry; isolation techniques; myocarditis; pathogens; polymerase chain reaction; spleen; tissues
Abstract:
... The bacterial pathogen Flavobacterium psychrophilum was successfully identified from formalin-fixed, wax-embedded tissue blocks of infected rainbow trout heart and spleen tissues, using a polymerase chain reaction (PCR)-based assay. Filamentous bacteria were observed in haematoxylin and eosin and Giemsa-stained sections but no bacteria were recovered from the diseased fish using standard bacteriol ...
DOI:
10.1111/j.1365-2761.2007.00779.x
PubMed:
17241403

31. Endophytic bacterial flora in the stem tissue of a tropical maize (Zea mays L.) genotype: isolation, identification and enumeration

Author:
Rai, Rhitu; Dash, Prasanta K.; Prasanna, B. M.; Singh, Aqbal
Source:
World journal of microbiology & biotechnology 2007 v.23 no.6 pp. 853-858
ISSN:
0959-3993
Subject:
Bacillus pumilus; Pseudomonas aeruginosa; Zea mays; bacteria; corn; cultivars; disinfestation; endophytes; fatty acid methyl esters; gas chromatography; genetic variation; genotype; greenhouse experimentation; greenhouses; growing season; population density; population dynamics; seedlings; tissues
Abstract:
... The genotypic diversity of indigenous bacterial endophytes within stem of tropical maize (Zea mays L.) was determined in field and greenhouse experiments. Strains were isolated from stem tissues of a tropical maize cultivar (PEHM-1) by trituration and surface disinfestation and their population dynamics was determined. Endophytes were found in most of the growing season at populations ranging from ...
DOI:
10.1007/s11274-006-9309-z

32. Isolation and characterization of endophytic bacteria from wild and cultivated soybean varieties

Author:
Hung, Pham Q.; Kumar, Senthil M.; Govindsamy, V.; Annapurna, K.
Source:
Biology and fertility of soils 2007 v.44 no.1 pp. 155-162
ISSN:
0178-2762
Subject:
Glycine max; bacteria; endo-1,4-beta-glucanase; endophytes; fluorescence; genetic variation; indole acetic acid; pigments; polygalacturonase; soybeans; stems; tissues
Abstract:
... Endophytic bacteria were isolated from surface sterilized stems, root, and nodules of wild and cultivated soybean varieties. Various phenotypic traits that are expected to be involved in the persistence and functions of the bacteria were analyzed. Most of the isolates from soybean were motile and indoleacetic acid producers, and 70 and 33% of isolates excreted cellulase and pectinase, respectively ...
DOI:
10.1007/s00374-007-0189-7

33. Lipidomics reveals control of Mycobacterium tuberculosis virulence lipids via metabolic coupling

Author:
Jain, Madhulika; Petzold, Christopher J.; Schelle, Michael W.; Leavell, Michael D.; Mougous, Joseph D.; Bertozzi, Carolyn R.; Leary, Julie A.; Cox, Jeffery S.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2007 v.104 no.12 pp. 5133-5138
ISSN:
0027-8424
Subject:
Mycobacterium tuberculosis; bacteria; biochemical pathways; fatty acids; malonyl coenzyme A; mass spectrometry; metabolism; mice; pathogens; tissues; virulence
Abstract:
... Mycobacterium tuberculosis synthesizes specific polyketide lipids that interact with the host and are required for virulence. Using a mass spectrometric approach to simultaneously monitor hundreds of lipids, we discovered that the size and abundance of two lipid virulence factors, phthiocerol dimycocerosate (PDIM) and sulfolipid-1 (SL-1), are controlled by the availability of a common precursor, m ...
DOI:
10.1073/pnas.0610634104
PubMed:
17360366
PubMed Central:
PMC1829275

34. Localization of granulatimide alkaloids in the tissues of the ascidian Didemnum granulatum

Author:
Mirna H. R. Seleghim; Simone P. de Lira; Patrícia T. Campana; Roberto G. S. Berlinck; Marcio R. Custódio
Source:
Marine biology 2007 v.150 no.5 pp. 967-975
ISSN:
0025-3162
Subject:
Ascidiacea; alkaloids; animals; antifeedants; bacteria; bladder; fluorescence; fluorescence microscopy; fungi; protective effect; secondary metabolites; sunscreens; symbionts; tissues
Abstract:
... Ascidians are a notable source of nitrogen-bearing secondary metabolites with a wide range of biological activities. Although many biologically active compounds have been isolated from ascidians, it is often unclear whether the animal or associated microbial symbionts such as bacteria or fungi are the true biosynthetic source of the metabolites. We have addressed the question of the biosynthetic s ...
DOI:
10.1007/s00227-006-0410-5

35. Molecular and biochemical characterization of galectin from amphioxus: primitive galectin of chordates participated in the infection processes

Author:
Yu, Yanhong; Yuan, Shaochun; Yu, Yingcai; Huang, Huiqing; Feng, Kaixia; Pan, Minming; Huang, Shengfeng; Dong, Meiling; Chen, Shangwu; Xu, Anlong
Source:
Glycobiology 2007 v.17 no.7 pp. 774-783
ISSN:
0959-6658
Subject:
Chordata; alternative splicing; bacteria; diverticulum; embryogenesis; exons; galectins; gills; host-pathogen relationships; immune response; immune system; intestines; introns; messenger RNA; phylogeny; quantitative polymerase chain reaction; recombinant proteins; tissue distribution; tissues
Abstract:
... A novel F4-carbohydrate recognition domain (CRD)-linker-F3-CRD-type bi-CRD Branchiostoma belcheri tsingtauense galectin (BbtGal)-L together with its alternatively spliced mono-CRD isoform BbtGal-S from amphioxus intestine was encoded by a 9488-bp unique gene with eight exons and seven introns. The recombinant proteins of BbtGal were found to have β-galactoside-binding activity, indicating that Bbt ...
DOI:
10.1093/glycob/cwm044

36. Occurrence of Alfalfa Bacterial Stem Blight Disease in Kurdistan Province, Iran

Author:
Harighi, B.
Source:
Phytopathologische Zeitschrift 2007 v.155 no.10 pp. 593-595
ISSN:
0931-1785
Subject:
Pseudomonas syringae pv. syringae; alfalfa; arginine deiminase; bacteria; blight; hypersensitivity; levan; pathogenicity; petioles; phenotype; potatoes; spring; summer; tissues; tobacco; Iran
Abstract:
... During spring and summer of 2004 and 2005, a new disease of alfalfa was observed for the first time in some areas of the Kurdistan province in Iran. Symptoms were initially yellowed area on leaves, within which water-soaked, irregular spots developed. These spots eventually coalesced to produce large necrotic areas. Symptoms on petiole and stem include water-soaked lesions, which later turned brow ...
DOI:
10.1111/j.1439-0434.2007.01284.x

37. Primary co-culture as a complementary approach to explore the diversity of bacterial associations in marine invertebrates: the example of Nautilus macromphalus (Cephalopoda: Nautiloidea)

Author:
Mathieu Pernice; Delphine Pichon; Isabelle Domart-Coulon; Jocelyne Favet; Renata Boucher-Rodoni
Source:
Marine biology 2007 v.150 no.5 pp. 749-757
ISSN:
0025-3162
Subject:
Cephalopoda; DNA; RNA; animal physiology; aquatic invertebrates; bacteria; beta-Proteobacteria; cell growth; coculture; genetic markers; microbial detection; models; tissues
Abstract:
... The recent application of molecular tools to address associations between bacteria and marine invertebrates has provided access to an immense diversity of unidentified microbes resistant to cultivation. However, the role of bacteria as partners in animal physiology remains unclear and in most cases difficult to investigate in the absence of adequate condition of cell growth and proliferation. In t ...
DOI:
10.1007/s00227-006-0413-2

38. Susceptibility of channel catfish, Ictalurus punctatus (Rafinesque), to Edwardsiella ictaluri challenge following copper sulphate exposure

Author:
Griffin, B.R.; Mitchell, A.J.
Source:
Journal of fish diseases 2007 v.30 no.10 pp. 581-585
ISSN:
0140-7775
Subject:
Edwardsiella ictaluri; Ictalurus punctatus; bacteria; bacterial adhesion; catfish; copper; copper sulfate; disease resistance; epithelial cells; freeze drying; mortality; tissues; toxicity
Abstract:
... Channel catfish, Ictalurus punctatus (Rafinesque), with or without a preliminary 24 h exposure to 2 mg copper sulphate L⁻¹, were challenged with 7.5 x 10⁶ colony forming units L⁻¹ of Edwardsiella ictaluri to determine the effect of copper sulphate on disease resistance. Catfish previously exposed to copper sulphate were significantly more resistant to the bacterial challenge than those not exposed ...
DOI:
10.1111/j.1365-2761.2007.00838.x
PubMed:
17850574

39. Attempts to revive Mycobacterium tuberculosis from 300-year-old human mummies

Author:
Lemma, Eshetu; Zimhony, Oren; Greenblatt, Charles L.; Koltunov, Viktoria; Zylber, María Inés; Vernon, Kim; Spigelman, Mark
Source:
FEMS microbiology letters 2008 v.283 no.1 pp. 54-61
ISSN:
0378-1097
Subject:
Mycobacterium tuberculosis; antibiotics; bacteria; chemical treatment; environmental factors; formalin; guinea pigs; humans; longevity; pasteurization; tissues
Abstract:
... Environmental persistence of Mycobacterium tuberculosis is subject to speculation. However, the reality that infected postmortem tissues can be a danger to pathologists and embalmers has worrisome implications. A few experimental studies have demonstrated the organism's ability to withstand exposure to embalming fluid and formalin. Recently, a failure was reported in an attempt to resuscitate an o ...
DOI:
10.1111/j.1574-6968.2008.01150.x
PubMed:
18399990

40. Construction of a stable GFP-tagged Vibrio harveyi strain for bacterial dynamics analysis of abalone infection

Author:
Travers, Marie-Agnès; Barbou, Annaïck; Le Goïc, Nelly; Huchette, Sylvain; Paillard, Christine; Koken, Marcel
Source:
FEMS microbiology letters 2008 v.289 no.1 pp. 34-40
ISSN:
0378-1097
Subject:
Haliotis tuberculata; Vibrio harveyi; abalone; bacteria; disease transmission; flow cytometry; fluorescence; hemolymph; models; mortality; parasites; pathogens; seawater; tissues; vertebrates; virulence; virulent strains
Abstract:
... Vibrio harveyi is a bacterial marine pathogen that can cause fatal disease in a large range of vertebrates and invertebrates, including the commercially important marine gastropod, Haliotis tuberculata. Since 1997, strains of this bacterium have regularly been causing high mortalities in farmed and wild abalone populations. The way in which the pathogen enters into abalone and the disease transmis ...
DOI:
10.1111/j.1574-6968.2008.01367.x
PubMed:
19054091

41. Differential regulation of two glutamine synthetase genes by a single Dof transcription factor

Author:
Rueda-López, Marina; Crespillo, Remedios; Cánovas, Francisco M.; Ávila, Concepción
Source:
Plant journal 2008 v.56 no.1 pp. 73-85
ISSN:
0960-7412
Subject:
Pinus pinaster; bacteria; biosynthesis; conifers; gene expression; genes; glutamate-ammonia ligase; glutamine; leaves; messenger RNA; models; oligonucleotide probes; protoplasts; repressor proteins; roots; shoots; tissues; tobacco; transcription (genetics); trees
Abstract:
... The PpDof5 transcription factor from maritime pine (Pinus pinaster) is a regulator of the expression of glutamine synthetase (GS) genes in photosynthetic and non-photosynthetic tissues. PpDof5 mRNA is detected almost ubiquitously during pine development with low levels of gene expression in green tissues and much higher levels in roots and lignified shoots. The PpDof5 protein expressed in bacteria ...
DOI:
10.1111/j.1365-313X.2008.03573.x
PubMed:
18547397

42. Digestive function of lysozyme in synanthropic acaridid mites enables utilization of bacteria as a food source

Author:
Erban, Tomas; Hubert, Jan
Source:
Experimental & applied acarology 2008 v.44 no.3 pp. 199-212
ISSN:
0168-8162
Subject:
Dermatophagoides farinae; Lepidoglyphus destructor; Micrococcus luteus; acarology; bacteria; cecum; cell walls; correlation; culture media; diet; digestion; fluorescein; insects; lysozyme; midgut; mites; pH; peptidoglycans; population growth; ruminant stomach; tissues
Abstract:
... The activity of lysozyme, the enzyme that hydrolyzes peptidoglycan in G⁺ bacterial cell walls, was detected in whole mite extracts (WME) and in spent growth medium extracts (SGME) of 14 species of synanthropic mites (Acari: Acaridida). The adaptation of lysozyme for digestive activity and bacteriophagy was based on: (i) high lysozyme activity in SGME, and (ii) the correlation of maximum lysozyme a ...
DOI:
10.1007/s10493-008-9138-x
PubMed:
18357505

43. Diversity and distribution of hemerythrin-like proteins in prokaryotes

Author:
French, Christopher E.; Bell, Jennifer M.L.; Ward, F. Bruce
Source:
FEMS microbiology letters 2008 v.279 no.2 pp. 131-145
ISSN:
0378-1097
Subject:
Archaea; amino acid sequences; aspartic acid; bacteria; bacterial proteins; body fluids; chemotaxis; eukaryotic cells; glutamic acid; histidine; histidine kinase; invertebrates; nucleotide sequences; oxygen; prokaryotic cells; signal transduction; surveys; tissues
Abstract:
... Hemerythrins are oxygen-binding proteins found in the body fluids and tissues of certain invertebrates. Oxygen is bound at a nonheme iron centre consisting of two oxo-bridged iron atoms bound to a characteristic set of conserved histidine: aspartate and glutamate residues with the motifs H-HxxxE-HxxxH-HxxxxD. It has recently been demonstrated biochemically that two bacterial proteins bearing the s ...
DOI:
10.1111/j.1574-6968.2007.01011.x
PubMed:
18081840

44. Effects of colonization, luminescence, and autoinducer on host transcription during development of the squid-vibrio association

Author:
Chun, Carlene K.; Troll, Joshua V.; Koroleva, Irina; Brown, Bartley; Manzella, Liliana; Snir, Einat; Almabrazi, Hakeem; Scheetz, Todd E.; de Fatima Bonaldo, Maria; Casavant, Thomas L.; Soares, M. Bento; Ruby, Edward G.; McFall-Ngai, Margaret J.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2008 v.105 no.32 pp. 11323-11328
ISSN:
0027-8424
Subject:
Danio rerio; Vibrio fischeri; bacteria; bioluminescence; evolution; gene expression regulation; genes; juveniles; mice; microarray technology; mutants; receptors; squid; symbionts; symbiosis; tissues; transcription (genetics); transcriptome
Abstract:
... The light-organ symbiosis between the squid Euprymna scolopes and the luminous bacterium Vibrio fischeri offers the opportunity to decipher the hour-by-hour events that occur during the natural colonization of an animal's epithelial surface by its microbial partners. To determine the genetic basis of these events, a glass-slide microarray was used to characterize the light-organ transcriptome of j ...
DOI:
10.1073/pnas.0802369105
PubMed:
18682555
PubMed Central:
PMC2516268

45. Genetic diversity of endophytic bacteria which could be find in the apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants

Author:
Velázquez, Encarna; Rojas, Marcia; Lorite, María José; Rivas, Raúl; Zurdo-Piñeiro, José Luis; Heydrich, Mayra; Bedmar, Eulogio J.
Source:
Journal of basic microbiology 2008 v.48 no.2 pp. 118-124
ISSN:
0233-111X
Subject:
Acinetobacter; Comamonas; DNA fingerprinting; Gluconacetobacter; Microbacterium; Micrococcus; Pantoea; Rhizobium; Staphylococcus; Xanthomonas; bacteria; bacterial communities; endophytes; genes; genetic variation; phylogeny; ribosomal RNA; sap; sugarcane; tissues; Cuba
Abstract:
... The genetic diversity of 29 endophytic bacterial strains isolated from apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants grown in Cuba was analysed by Two Primers-Ramdom Amplified Polymorphic DNA fingerprinting (TP-RAPD) and 16S rRNA gene sequencing. The strains were distributed into 17 groups on the basis of their TP-RAPD patterns, and a representative strain from ...
DOI:
10.1002/jobm.200700161

46. Genome analysis of the coral bleaching pathogen Vibrio shiloi

Author:
Reshef, Leah; Ron, Eliora; Rosenberg, Eugene
Source:
Archives of microbiology 2008 v.190 no.2 pp. 185-194
ISSN:
0302-8933
Subject:
Vibrio mediterranei; Vibrio shiloi; adhesion; bacteria; coral bleaching; corals; fimbriae; genes; genomic islands; occludins; pathogens; sequence analysis; superoxide dismutase; suppression subtractive hybridization; tissues; virulence
Abstract:
... The past few decades have seen a world-wide increase in coral diseases, yet little is known about coral pathogens. In this study, techniques commonly used in pathogenomic research were applied to the coral pathogen Vibrio shiloi in order to identify genetic elements involved in its virulence. Suppressive subtractive hybridization was used to compare the gene content of V. shiloi to that of a close ...
DOI:
10.1007/s00203-008-0388-0

47. The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants

Author:
Gutierrez, Laurent; Mauriat, Mélanie; Guénin, Stéphanie; Pelloux, Jérôme; Lefebvre, Jean-François; Louvet, Romain; Rusterucci, Christine; Moritz, Thomas; Guerineau, François; Bellini, Catherine; Van Wuytswinkel, Olivier
Source:
Plant biotechnology journal 2008 v.6 no.6 pp. 609-618
ISSN:
1467-7644
Subject:
Arabidopsis thaliana; Populus tremula; Populus tremuloides; algorithms; bacteria; essential genes; gene expression; hybrids; mammals; models; reverse transcriptase polymerase chain reaction; tissues; transcriptome; yeasts
Abstract:
... Reverse transcription-polymerase chain reaction (RT-PCR) approaches have been used in a large proportion of transcriptome analyses published to date. The accuracy of the results obtained by this method strongly depends on accurate transcript normalization using stably expressed genes, known as references. Statistical algorithms have been developed recently to help validate reference genes, and mos ...
DOI:
10.1111/j.1467-7652.2008.00346.x
PubMed:
18433420

48. A BAHD acyltransferase is expressed in the tapetum of Arabidopsis anthers and is involved in the synthesis of hydroxycinnamoyl spermidines

Author:
Grienenberger, Etienne; Besseau, Sébastien; Geoffroy, Pierrette; Debayle, Delphine; Heintz, Dimitri; Lapierre, Catherine; Pollet, Brigitte; Heitz, Thierry; Legrand, Michel
Source:
Plant journal 2009 v.58 no.2 pp. 246-259
ISSN:
0960-7412
Subject:
RNA interference; flowering; genes; pollen; methylation; recombinant proteins; males; secondary metabolites; genotype; esters; antiserum; metabolomics; tissues; Arabidopsis thaliana; buds; gene overexpression; spermidine; hydroxycinnamoyltransferase; immunoblotting; flowers; tandem mass spectrometry; acylation; mutants; methyltransferases; bacteria
Abstract:
... BAHD acyltransferases catalyze the acylation of many plant secondary metabolites. We characterized the function of At2g19070, a member of the BAHD gene family of Arabidopsis thaliana. The acyltransferase gene was shown to be specifically expressed in anther tapetum cells in the early stages of flower development. The impact of gene repression was studied in RNAi plants and in a knockout (KO) mutan ...
DOI:
10.1111/j.1365-313X.2008.03773.x
PubMed:
19077165

49. A highly virulent pathogen, Aeromonas hydrophila, from the freshwater crayfish Pacifastacus leniusculus

Author:
Jiravanichpaisal, Pikul; Roos, Stefan; Edsman, Lennart; Liu, Haipeng; Soderhall, Kenneth
Source:
Journal of invertebrate pathology 2009 v.101 no.1 pp. 56-66
ISSN:
0022-2011
Subject:
Aeromonas hydrophila; Pacifastacus leniusculus; animal pathogens; aquatic plants; bacteria; crayfish; cytotoxicity; endotoxins; freshwater; genes; heart; hematopoietic stem cells; hemocytes; hepatopancreas; histopathology; humans; in vitro studies; mortality; temperature; tissues; virulence
Abstract:
... Aeromonas spp. are characteristic bacteria of freshwaters and many of them can be components of the bacterial flora of aquatic animals and may become pathogens on animals including humans. In this study Aeromonas hydrophila was isolated from the freshwater crayfish, Pacifastacus leniusculus, and was found to be a highly pathogenic bacterium among many isolated bacteria. Mortality reached 100% with ...
DOI:
10.1016/j.jip.2009.02.002

50. A redox-active isopropylmalate dehydrogenase functions in the biosynthesis of glucosinolates and leucine in Arabidopsis

Author:
He, Yan; Mawhinney, Thomas P.; Preuss, Mary L.; Schroeder, Amy C.; Chen, Bing; Abraham, Linda; Jez, Joseph M.; Chen, Sixue
Source:
Plant journal 2009 v.60 no.4 pp. 679-690
ISSN:
0960-7412
Subject:
Arabidopsis; bacteria; biosynthesis; chloroplasts; decarboxylation; enzymes; gene expression regulation; genes; glucosinolates; leucine; methionine; mutants; mutation; phenotype; substrate specificity; tissues; yeasts
Abstract:
... We report a detailed functional characterization of an Arabidopsis isopropylmalate dehydrogenase (AtIPMDH1) that is involved in both glucosinolate biosynthesis and leucine biosynthesis. AtIPMDH1 shares high homology with enzymes from bacteria and yeast that are known to function in leucine biosynthesis. In plants, AtIPMDH1 is co-expressed with nearly all the genes known to be involved in aliphatic ...
DOI:
10.1111/j.1365-313X.2009.03990.x
PubMed:
19674406