An official website of the United States government
Here’s how you know
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
... Sulfur is an essential element for life. However, the soil microbe Pseudomonas (P.) fluorescens can survive in a low sulfur environment. When cultured in a sulfur-deficient medium, the bacterium reprograms its metabolic pathways to produce α-ketoglutarate (KG) and regenerate this keto-acid from succinate, a by-product of ROS detoxification. Succinate semialdehyde dehydrogenase (SSADH) and KG decar ...
... Surfactin is a bacterial lipopeptide and an influential biosurfactant mainly known for excellent surfactant ability. The amphiphilic nature of surfactin helps it to sustain under hydrophobic and hydrophilic conditions. In this investigation, a bacterium strain (BTKU3) that produces biosurfactant were isolated from oil-contaminated soil. Based on the blue agar plate (Bap) assay, the BTKU3 strain wa ...
... Siderite was applied to the binary oxidant system of siderite-catalyzed hydrogen peroxide (H₂O₂) and enhanced with persulfate (PS). In the absence of PS, methyl orange (MO) almost could not be degraded by the siderite/H₂O₂ process. However, adding PS significantly improved the capacity of MO to oxidize azo-dye. The influence of individual and interaction of reaction factors have been explored with ...
... Iron-manganese binary oxides (Fe-Mn oxides) have the potential to immobilize arsenite/arsenate [As(III/V)] in situ in natural environments. However, the As(III/V) immobilization performances of Fe-Mn oxides in the presence of indigenous metal-reducing bacteria in reducing groundwater systems have received limited attention. Here, As(III/V) immobilization by Fe-Mn oxides and the interactions of Fe ...
Enterobacter aerogenes; Eucheuma; ammonium sulfate; asparaginase; bacteria; dialysis; dietary supplements; drugs; endophytes; enzyme activity; gel chromatography; ion exchange chromatography; pH; protein content; temperature; Indonesia
Abstract:
... This study was aimed to purify and characterize the enzyme L‐asparaginase from endophytic microorganisms of Eucheuma sp. in Puntondo Island, South Sulawesi, Indonesia. Research stages were ammonium sulfate precipitation, dialysis, and Sephadex G‐75 gel filtration chromatography and Sephadex CMC‐50 ion‐exchange chromatography matrices. The result of test activities of ammonium sulfate fraction show ...
... Coral reefs are rapidly declining because of widespread mass coral bleaching causing extensive coral mortality. Elevated seawater temperatures are the main drivers of coral bleaching, and climate change is increasing the frequency and severity of destructive marine heatwaves. Efforts to enhance coral thermal bleaching tolerance can be targeted at the coral host or at coral-associated microorganism ...
... Red palm weevil, Rhynchophorus ferrugineus, is an invasive and destructive pest that causes serious damages to palm trees. Like other invertebrates, red palm weevil relies solely on its innate immune response to fight invading microbes; by definition, innate immunity lacks adaptive characteristics. However, we show here that priming the red palm weevil larvae with heat-killed Bacillus thuringiensi ...
... Listeria monocytogenes is a ubiquitous Gram-positive foodborne pathogen that is responsible for listeriosis in both humans and several animal species. The bacterium secretes a pore-forming cholesterol-dependent cytolysin, listeriolysin O (LLO), a major virulence factor involved in the activation of cellular processes. The ability of LLO to lyse erythrocytes is a measure of LLO activity. We used he ...
Agrobacterium radiobacter; Chromobacterium violaceum; Vibrio fluvialis; bacteria; biosensors; gene expression; homoserine; lactones; opportunistic pathogens; population density; tandem mass spectrometry; ultra-performance liquid chromatography; violacein; East China Sea
Abstract:
... Vibrio fluvialis is a marine opportunistic pathogen that frequently causes diseases in aquatic animals and humans. V. fluvialis can produce quorum sensing signaling molecules to coordinate cell density-dependent behavioral changes, including N-acyl homoserine lactone (AHL), which acts as a vital mediator of virulence-associated gene expression. Currently, several AHL molecules in V. fluvialis have ...
... Accurate and online quantification of viable cells is one of the necessary requirements during the microbial fermentation process for high productivity. The flow cytometry (FCM)-based method accurately quantifies viable cells, but this offline method cannot reflect the counts constantly. The dielectric spectroscopy (DS) sensor is widely utilized to monitor viable cells online; however, accurately ...
... Cellulase enzymes have wide range of industrial application, but high production cost and relatively low efficiency are the main issues, which are needed to resolve. Substrate is known as the main contributor which can bring down the production cost of these enzymes at large scale. Therefore, in the present study, corn cob (Cc) waste has been employed as a potential substrate to produce efficient ...
... From 45 private fish farms, 641 tilapia samples were collected, which suffered from tilapia summer mortality outbreaks at different localities. The findings of the conventional test and 16S rRNA gene sequence recognition indicated that the isolated bacterium from the examined farms is Aeromonas veronii. A 15‐day feeding trial was conducted to determine the effects of formic acid (FA) on biochemica ...
... Triclocarban (TCC) is an emerging and intractable environmental contaminant due to its hydrophobicity and chemical stability. However, the antibacterial property of TCC limits its biodegradation, and only the functional enzyme TccA involved in TCC degradation has been characterized to date. In this study, we report a highly efficient TCC-degrading bacterium, Rhodococcus rhodochrous BX2, that could ...
... Shigella flexneri is the main causative agent of the communicable diarrheal disease, shigellosis. It is estimated that about 80–165 million cases and > 1 million deaths occur every year due to this disease. S. flexneri causes dysentery mostly in young children, elderly and immunocompromised patients, all over the globe. Recently, due to the emergence of S. flexneri antibiotic resistance strains, i ...
... The redclaw crayfish (Cherax quadricarinatus) is a freshwater decapod crustacean, cultured in numerous countries worldwide for both food and ornamental purposes. Redclaw crayfish has become an important aquaculture species due to its physical and biological traits, relatively easy breeding, and a short growing‐out period to reach commercial size. Bacterial infections are the second‐most studied pa ...
... The human diarrheal disease cholera is caused by the bacterium Vibrio cholerae. Efforts to develop animal models that closely mimic cholera to study the pathogenesis of this disease began >125 years ago. Here, we review currently used non-surgical, oral inoculation-based animal models for investigation of V. cholerae intestinal colonization and disease and highlight recent discoveries that have il ...
... To evaluate the antibacterial effect of Khadirarishta (An Ayurvedic traditional formulation) against some of the bacterial strains as well as to investigate the probable mechanism of action against sensitive bacterial strains by using standard procedures. The antibacterial activity of Khadirarishta against some of the bacterial strains was evaluated by using bacterial susceptibility assay and the ...
... The objective of this study was to investigate the antibacterial activity and potential mechanism of cyclolinopeptides, a type of cyclic hydrophobic peptides present in flaxseed oil. In this study, 1-Mso cyclolinopeptides B and 1-Mso, 3-Mso-cyclolinopeptides F from flaxseed oil exhibited excellent antibacterial activity against Listeria monocytogenes through destroying bacterial cell membrane. Our ...
Betty Mancebo Dorvigny; Lethicia Souza Tavares; Ingrydt Alcântara de Almeida; Lucas Nunes Santana; Esther de Souza Silva; Juliana Kelly Urtigas de Souza; Anísio Francisco Soares; Valdemiro Amaro da Silva Júnior; José Vitor Lima‐Filho
... Caffeine has been reported for its antiinflammatory properties by stimulating phagocytosis. In this study, we investigated the antiinflammatory and antiinfective potential of caffeine in murine macrophage cell cultures and Swiss mice infected with virulent Salmonella enterica serotype typhimurium. Peritoneal macrophages (pMØ) were treated with caffeine on 96‐well plates for 24 hr and then infected ...
... BACKGROUND: Listeria monocytogenes is a widespread common contaminant in food production facilities during preparation, storage, and distribution, and minimally processed ready‐to‐eat products are considered at high risk of contamination by this bacterium. Increased antibiotic resistance has led researchers to search for plant‐based natural alternatives to control pathogenic microorganisms. Among ...
... European plum (Prunus domestica L.) is a tree cultivated in many countries for its edible fruits, and necessarily certain amounts of useless woody residues are generated every year during pruning works. The objectives of this research were to recover the phenolics present on that wood wastes and study their antimicrobial and antibiofilm activities against a selection of foodborne microorganisms, i ...
... We isolated Pseudoalteromonas xiamenensis STKMTI.2 from the mangrove sediments of Setokok Island, Indonesia. This study aimed to identify the bacterium, investigate antagonistic properties and minimum inhibitory concentration, and detect the active compounds using gas chromatography–mass spectrometry. The bacterium was identified using a biochemical test and 16S rDNA sequence analysis. Bioactive c ...
... Chemical fertilizers are used in modern agricultural practice to increase plant output. They possess anthropogenic compounds which are hazardous, result in poor soil quality, poor crop nutrition and pollutes the water table. Currently, food crops that lack in micro-nutrients (Zn, silicates and Se) can be enriched with micronutrients by use of fertilizers. Eco-friendly bio-fertilizers have been pro ...
... The advent of genetically modified crops expressing insecticidal proteins of the bacterium Bacillus thuringiensis (Bt) revolutionized the management of insect pest species. Nonetheless, such disruption does not come free from controversies and concerns. The quick spread of Bt resistance in targeted pest species is an example, and the impact in non‐targeted arthropod species is another. The former ...
... Bacterial kidney disease (BKD) can be a devastating bacterial infection in salmonids, and it is present in aquaculture throughout the world. BKD is caused by the Gram‐positive facultative intracellular bacterium Renibacterium salmoninarum (R. salmoninarum) that is spread both horizontally and vertically. Disease signs include external ulcerations and blisters and internal signs such as organ swell ...
... The study of peptidoglycan-binding proteins frequently requires in vitro binding assays, in which the isolated peptidoglycan used as a substrate must be carefully quantified. Here, we describe an easy and sensitive assay for peptidoglycan quantification based on a modified Nelson–Somogyi reducing sugar assay. We report the response of this assay to different common sugars and adapt its use to pept ...
... The development of alternatives to dairy products has gained great importance using plant-based foods. In this study, efforts have been made to develop vegetable alternatives from soybean paste without any supplement using single potential probiotic culture. Commercial soybean meal was inoculated with Bifidobacterium (B.) longum under previously fitted fermentation conditions by experimental desig ...
Anandita Pal; Shan Sun; Michael Armstrong; Jonathan Manke; Nicole Reisdorph; Victoria R. Adams; Arion Kennedy; Yujiao Zu; Naima Moustaid-Moussa; Ian Carroll; Saame Raza Shaikh
... Eicosapentaenoic acid (EPA) ethyl esters are of interest given their clinical approval for lowering circulating triglycerides and cardiometabolic disease risk. EPA ethyl esters prevent metabolic complications driven by a high fat diet in male mice; however, their impact on female mice is less studied. Herein, we first investigated how EPA influences the metabolic profile of female C57BL/6J mice co ...
... The pathogenic bacterium Vibrio vulnificus is related to human infections by direct contact with the bacteria or by consuming raw aquacultural products, like oysters and clams. This work aimed to measure the effect of the microalga Isochrysis galbana on the density of two pathogenic V. vulnificus strains when they are co-cultured. Vibrio vulnificus strains CICESE-316 and CICESE-325 were inhibited ...
... The overuse of chloramphenicol (CAP) due to its low price is detrimental to ecological safety and human health. An earthworm gut content dwelling bacterium, Aeromonas media SZW3, was isolated with capability of CAP biodegradation, and the CAP degradation efficiency reached 55.86% at day 1 and 67.28% at day 6. CAP biodegradation kinetics and characteristic of strain SZW3 determined the factors that ...
... Di-(2-ethylhexyl) phthalate (DEHP), the most extensively used phthalate acid esters (PAEs), poses a potential risk to human and environment. A novel bacterial strain, Rhodococcus sp. LW-XY12, with efficient PAE-degrading capability, was isolated from activated sludge. Strain LW-XY12 could degrade 96.91 ± 0.68% of DEHP (500 mg L⁻¹) within 32 h. The degradation curves of DEHP (50–1500 mg L⁻¹) and PA ...
... Sulfoxaflor (SUL), a novel sulfoximine insecticide, causes environmental contamination and poses potential risks to animals. However, there are few reports of SUL degradation by pure microbes. In this study, a novel isolated bacterium, Pseudomonas stutzeri CGMCC 22915, rapidly degraded SUL to SUL-amide via hydration. P. stutzeri CGMCC 22915 degraded 58.2% of SUL (814.28 μmol/L) within 2 h with a h ...
... Actinobacteria have many properties that make them good candidates for the bioremediation of sites contaminated by several organic and inorganic pollutants. However, studies on the biodegradation of used motor oils by Actinobacteria, compared to other bacteria, remain little studied. Actinobacteria were isolated from soil contaminated with used motor oils and sewage sludge in order to select a spe ...
... Biosurfactants are non-toxic, surface-active biomolecules capable of reducing surface tension (ST) and emulsifying interface at a comparably lower concentration than commercial surfactants. Yet, poor yield, costlier substrates, and complex cultivation processes limit their commercial applications. This study focuses on producing biosurfactants by Pseudomonas aeruginosa P7815 in batch and fed-batch ...
... Bensulfuron-methyl (BSM) residues in soil threaten the rotation of BSM-sensitive crops. Microbial biofilms formed on crop roots could improve the ability of microbes to survive and protect crop roots. However, the research on biofilms with the purpose of mitigating or even eliminating BSM damage to sensitive crops is very limited. In this study, one BSM-degrading bacterium, Hansschlegelia zhihuaia ...
... AIMS: Carbapenem‐resistant Acinetobacter baumannii represents a public health problem, and the search for new antibacterial drugs has become a priority. Here, we investigate the antibacterial activity of biogenic silver nanoparticles (Bio‐AgNPs) synthesized by Fusarium oxysporum, used alone or in combination with polymyxin B against carbapenem‐resistant A. baumannii. METHODS AND RESULTS: In this s ...
... A novel Cr(VI)-resistant haloalkaliphilic bacterial strain NRC-R, identified as Salipaludibacillus agaradhaerens, was isolated from hypersaline soda lakes and characterized for its Cr(VI) bioreduction efficiency. Strain NRC-R grew well and effectively reduced Cr(VI) under a wide range of sodium chloride, pH, shaking velocity and temperature, showing maximum Cr(VI) reduction at 8% NaCl, pH 10, 150 ...
Cicer arietinum; bacteria; biochemical oxygen demand; biodegradation; bioremediation; chemical oxygen demand; chemical pollutants; color; decolorization; environmental technology; glucose; high performance liquid chromatography; metabolites; pH; peptones; phytotoxicity; sludge; sustainable development; temperature; India
Abstract:
... In India, the environment friendly and sustainable management of colored effluents emanating from alcohol-producing distilleries has become a serious environmental concern. The current study aimed to examine the efficiency of ligninolytic enzyme-producing bacterial strain isolated from ameliorated distillery sludge by enrichment method for its bioremediation and detoxification potential of biometh ...
... Guanidinoacetate (GAA) is a naturally occurring amino acid derivative and the direct precursor of creatine, which is widely used in feed additives and the pharmaceutical industry. The current industrial synthesis of GAA is based on chemical methods, which limits the application of GAA. Here, a biological approach is developed for food safety GAA production via whole-cell biocatalysis by the genera ...
... Klebsiella pneumoniae is a 2,3-butanediol producing bacterium. Nevertheless, a design and construction of L-valine production strain was studied in this paper. The first step of 2,3-butanediol synthesis and branched-chain amino acid synthesis pathways share the same step of α-acetolactate synthesis from pyruvate. However, the two pathways are existing in parallel and do not interfere with each oth ...
... Strain Marseille-P9829 was isolated from a bone sample collected from an open right fibula fracture from a 46-years old patient. Strain Marseille-P9829 (= CSUR P9829 = DSM 110695) was a Gram-negative, non-spore-forming and non-motile bacterium. This strain had a positive catalase activity but was oxidase-negative. The major fatty acids methyl esters were hexadecanoic acid (45.6%) and 9-hexadecenoi ...
... A new focus on biofiltration has emerged that aims to shape microbial communities to improve treatment efficacy. It is therefore necessary to understand the linkages between microbial community structure and biofilter function. However, the assembly and interaction of microbial communities in biological activated carbon (BAC) filters are unknown. In this study, we selected one coal-based granular ...
... A large (47.75 ± 3.56 µm in diameter) Thiovulum bacterial strain forming white veils is described from a marine mangrove ecosystem. High sulfide concentrations (up to 8 mM of H₂S) were measured on sunken organic matter (wood/bone debris) under laboratory conditions. This sulfur-oxidizing bacterium colonized the organic matter, forming a white veil. According to conventional scanning electron micro ...
... In the present study, E. coli was taken as a model bacterium, anti-E. coli functionalized magnetic beads were constructed and used to capture E. coli from aqueous extracts of fish sarcoplasmic protein (FSP) and fish muscle protein of sablefish. The excellency of the reproducibility of the present protocol was demonstrated by capturing E. coli from sablefish FSP extracts. The presence of 10 CFU/mL ...
... The immobility of phosphorus (P) and its precipitation in the soil is a major fact and remains in place where P fertilizer applied. Without organic amendments and beneficial microbes, P availability is limited in the current season crop. To increase crop productivity and P availability, field experiments were conducted at Agriculture Research Institute Mingora Swat (ARI) for two consecutive years ...
... The objective of this work is to elucidate the mechanism of tetracycline (TC) removal by Pseudomonas sp. TC952. The TC removal characteristics of strain TC952 under various environmental conditions were studied. Results showed that the bio-removal efficiency was significantly affected by initial TC and peptone concentration, pH values, divalent metal ion (Zn²⁺) and carbon source, and the strain TC ...
... BACKGROUND: To date, Campylobacter jejuni has not been found to be pathogenic to peafowl. The available publications show that out of a total of 44 samples tested from peafowl, this bacterium was isolated only in two cases. Eimeria pavonina infestations in the peafowl have been described, but no fatal cases have been reported yet. CASE PRESENTATION: The four-year-old peacock was presented with chr ...
... BACKGROUND: Enterobacter hormaechei is typically a opportunistic pathogenic bacterium in humans, and no pathological change of of Enterobacter hormaechei in diseased sheep has previously been documented. CASE PRESENTATION: Three free-range, four-month-old female sheep were ill with respiratory disease and died three days after receiving treatment with ceftiofur sodium. A frozen lung sample of one ...
... Peptidoglycan recognition proteins (PGRPs) participate in insect defense against bacterial pathogens by recognizing bacterial cell wall peptidoglycans (PGNs). Here, we identified the PGRP-LB gene in the white-backed planthopper Sogatella furcifera (SfPGRP-LB). SfPGRP-LB is a secreted protein with a typical PGN-binding domain and five conserved amino acid (aa) residues required for amidase activity ...
... Zymomonas mobilis ZM4 is a gram-negative, facultative anaerobic, natural ethanologenic bacterium used in industrial production of bio-products. For expression of genes, promoters are required. However, most of the promoters reported from Z. mobilis poorly function in Escherichia coli. This makes the process of expression and screening labor-intensive. In the present study, we compared the strength ...
Holothuroidea; Streptomyces cavourensis; bacteria; bacterial culture; calcium; cations; chemical structure; color; melanin; polymers; water solubility
Abstract:
... Melanin is a widely distributed and striking dark-colored pigment produced by countless living organisms. Although a wide range of bioactivities have been recognized, there are still major constraints in using melanin for biotechnological applications such as its fragmentary known chemical structure and its insolubility in inorganic and organic solvents. In this study, a bacterial culture of Strep ...
... The sulfur-containing odor emitted from sludge composting could be controlled by sulfide oxidizing bacteria, yet mesophilic strains show inactivation during the thermophilic stage of composting. Aimed to investigate and characterize the thermotolerant bacterium that could oxidize sulfide into sulfate, a heterotrophic strain was isolated from sewage sludge composting and identified as Paenibacillus ...
... In the context of algal wastewater bioremediation, this study has identified a novel consortium formed by the bacterium Methylobacterium oryzae and the microalga Chlamydomonas reinhardtii that greatly increase biomass generation (1.22 g L⁻¹·d⁻¹), inorganic nitrogen removal (>99%), and hydrogen production (33 mL·L⁻¹) when incubated in media containing ethanol and methanol. The key metabolic aspect ...
... Three moderately halophilic strains, TMW 2.2308ᵀ, TMW 2.2299 and TMW 2.2304, were isolated from a lupine-based moromi fermentation. Initial identification based on their low molecular sub-proteome using mass spectrometry showed relation to the genus Halomonas, however, low score values indicated novelty. The comparison of 16S rRNA gene sequences placed these strains within the genus Chromohalobact ...
... The clinical relevance of Helicobacter heilmannii (H. heilmannii) infection in patients with upper gastrointestinal diseases is not yet fully understood. The main purpose of this study was to evaluate the clinical, endoscopic, histopathological, and therapeutic features of H. heilmannii. To retrieve the relevant studies, we conducted a comprehensive systematic search of global databases such as th ...
... The fate and transport of perfluorinated compounds (PFCs) have been extensively studied and widely reviewed recently. Literature reports that they are persistent and may travel considerable distances in all environmental compartments, such as water, sediment, air, and soil owing to their chemical and thermal stability. However, their transport with co-existing contaminants and their potential as v ...
... Gluconacetobacter diazotrophicus is a nitrogen (N) fixing bacterium originally isolated from sugarcane in Brazil. Understanding the interactions between this bacterium and plants is important to exploit the nitrogen-fixing feature of G. diazotrophicus in different crops. Research was conducted to establish, monitor and optimize methods for introducing G. diazotrophicus into Brachypodium distachyon ...
... Groundwater contamination by recalcitrant organic micropollutants such as pesticide residues poses a great threat to the quality of drinking water. One way to remediate drinking water containing micropollutants is to bioaugment with specific pollutant degrading bacteria. Previous attempts to augment sand filters with the 2,6-dichlorobenzamide (BAM) degrading bacterium Aminobacter niigataensis MSH1 ...
... Escherichia coli, a bacterium that causes severe foodborne diseases, is transmitted to humans primarily through the consumption of contaminated foods. These foodborne pathogens are causing a public health problem that requires alternative control approaches, such as bacteriophage (phage) biocontrol. In this study, we characterized vB_EcoM_Tw01 (vTw01) isolated from sewage and vB_EcoM_Tcm05 (vTcm05 ...
... Rhodococcus pyridinivorans B403 is a promising bacterium for degrading phenolic pollutants. In the application, the high-concentration substrate has a significant inhibitory effect on cell growth and phenol degradation, which makes adaptive evolution study of bacteria an important guarantee for further application. The present work found evolved R. pyridinivorans (X1 and X2) had enhanced tolerance ...
Vibrio cholerae; bacteremia; bacteria; cholera; cholera toxin; cross resistance; diarrhea; fish; food safety; liquid chromatography; pandemic; pathogenesis; pathogens; proteome; proteomics; shellfish; tandem mass spectrometry; two-dimensional gel electrophoresis; vaccines; virulence
Abstract:
... Vibrio cholerae can cause pandemic cholera in humans. The bacterium resides in aquatic environments worldwide. Identification of risk factors of V. cholerae in aquatic products is imperative for assuming food safety. In this study, we determined virulence-associated genes, cross-resistance between antibiotics and heavy metals, and genome fingerprinting profiles of non O1/O139 V. cholerae isolates ...
Elena S. Baena-Santillán; Javier Piloni-Martini; Esmeralda Rangel-Vargas; Carlos A. Gómez-Aldapa; Manuel Sánchez-Gutiérrez; Eduardo O. Madrigal-Santillán; Javier Castro-Rosas
... The main aim of this study was to determine and compare the antimicrobial effect of hibiscus acid and a commercial 0.12% (w/v) chlorhexidine mouthrinse against Streptococcus mutans, Streptococcus sanguinis, Capnocytophaga gingivalis, and Staphylococcus aureus, and to determine the effect on bacterial cell membrane permeability and the toxicity of hibiscus acid in a mouse model. Hibiscus acid was o ...
... Xanthomonas arboricola pv. corylina is the causal agent of bacterial blight of hazelnut. The bacterium has been listed as an A2 quarantine pathogen in Europe since 1978 and on the regulated non-quarantine pest list since 2019. Three isolates from various geographic regions and isolated at different times were sequenced using a hybrid approach with short- and long-read technologies to generate clos ...
... The Bacillus velezensis YYC strain was isolated from the tomato rhizosphere. In a previous experiment, it increased tomato growth and induced systemic resistance against Ralstonia solanacearum. However, information on its genomic content is lacking. The complete genome sequence of the bacterium was described in this study. The genome size was 3,973,236 bp and consisted of 4034 genes in total, with ...
... Roseivivax marinus strain TCYB24 is a rod-shaped bacterium of Rhodobacteraceae isolated from the gill of deep-sea mussel Bathymodiolus marisindicus which collected from the Tiancheng hydrothermal vent under depth of 2700 m on the southwest Indian ridge. In our previous study, the strain TCYB24 was proved to produce quorum sensing signal of N-Acyl-homoserine lactones (AHLs) and form biofilm. In ord ...
... Parasedimentitalea marina W43ᵀ is a novel psychrotolerant and piezotolerant Rhodobacteraceae bacterium isolated from deep-sea water (4000 m) of the New Britain Trench. Here we present the first complete genome sequence of the bacterial genus Parasedimentitalea, which contains a circular chromosome and four plasmids. The 5,080,916 bp long genome exhibits a G + C content of 55.9 mol% and contains 50 ...
... The lyophilization process is the most convenient and successful method to preserve probiotics, although microorganisms are exposed to conditions of extremely low freezing temperatures as well as dehydration. In this study, we evaluated the cryoprotective effect of turanose on Lactobacillus paracasei subsp. paracasei, L. casei 431 (L. casei 431) as a method to increase survival rate by improving c ...
... Cupriavidus metallidurans is a model bacterium to study molecular metal resistance mechanisms and its use for the bioremediation of several metals has been shown. However, its mechanisms for radionuclide resistance are unexplored. We investigated the interaction with uranium and associated cellular response to uranium for Cupriavidus metallidurans NA4. Strain NA4 actively captured 98 ± 1% of the u ...
bacteria; cell walls; multiple drug resistance; oxidative stress; public health; reactive oxygen species; toxicity
Abstract:
... The emergence of multidrug-resistant (MDR) bacteria in recent years has been alarming and represents a major public health problem. The development of effective antimicrobial agents remains a key challenge. Nanotechnologies have provided opportunities for the use of nanomaterials as components in the development of antibacterial agents. Indeed, metal-based nanoparticles (NPs) show an effective rol ...
... A. baumannii is a multi-drug resistant pathogen with a relatively high mortality rate. To date, no vaccine has been approved against this bacterium. DcaP is a high abundance porin during infection that its structure has been recently determined, but no information about its immunogenic properties has been reported yet. So, in this study DcaP properties were analyzed and its vaccine potential was e ...
... Polychlorinated dibenzo-p-dioxins and furans (PCDD/F) are some of the most environmentally recalcitrant and toxic compounds. They occur naturally and as by-products of anthropogenic activity. Sydney Harbour Estuary (Sydney, Australia), is heavily contaminated with PCDD/F. Analysis of sediment cores revealed that the contamination source area in Homebush Bay continues to have one of the highest lev ...
... Herein, we present a facile and sensitive fluorescence resonance energy transfer (FRET) aptasensor for the detection of pathogenic bacteria, where antibiotic-functionalized cerium oxide nanoparticles were served as an energy donor and aptamer-modified gold nanoparticles (aptamer-AuNPs) were employed as an energy acceptor. To illustrate the feasibility of this strategy, Escherichia coli (E. coli) w ...
Bacillus subtilis; agar; bacteria; biosensors; biotechnology; dose response; fluorescence; green fluorescent protein; liquids; metabolites; multiple drug resistance; polymyxin B
Abstract:
... It is an urgent need to develop novel antibiotics to treat infections caused by multi-drug-resistant bacteria. One promising strategy could be the use of whole-cell biosensors, which have been extensively studied to monitor environmental pollutants and intracellular metabolites. Here, we used the σᴹ-mediated regulatory system of Bacillus subtilis to construct a whole-cell biosensor for the detecti ...
Hyeon‐Je Cho; York Moy; Nathan A. Rudnick; Theodore M. Klein; Jiaming Yin; Joy Bolar; Carol Hendrick; Mary Beatty; Leandro Castañeda; Anthony J. Kinney; Todd J. Jones; N. Doane Chilcoat
... We have discovered a novel bacterium, Ochrobactrum haywardense H1 (Oh H1), which is capable of efficient plant transformation. Ochrobactrum is a new host for Agrobacterium‐derived vir and T‐DNA‐mediated transformation. Oh H1 is a unique, non‐phytopathogenic species, categorized as a BSL‐1 organism. We engineered Oh H1 with repurposed Agrobacterium virulence machinery and demonstrated Oh H1 can tra ...
Heba H. Mahboub; Hassnaa Mahmoud Elsheshtawy; Nagwa I. Sheraiba; Esra.M. Fahmy; Shimaa R. masoud; Eman A.A. Mohamed; Noha S. Abdelnaeim; Dalia Ibrahim Mohamed; Tamer Ahmed Ismail; Shaimaa A.A. Ahmed
... The present study assessed the protective aspects of the dietary exposure to black cumin (Nigella sativa, NS) powder on immune response, liver and kidney enzymes, tissue antioxidant capacity, gene expression, and testing the antibacterial activity against Burkholderia cepacia (B. cepacia, BC). Nile tilapia (O. niloticus) (n = 150) were divided into 5 groups, the first was control (Cont) neither ch ...
Bacteroidetes; Clostridium; Vigna radiata; bacteria; colon; food science; human health; intestinal microorganisms; metabolites; mung beans; polysaccharides; probiotics; technology; water solubility
Abstract:
... Cumulative evidence revealed that the gut microbiota play important role in human health. Polysaccharide from Mung bean [Vigna radiate (Linn.) Wilczek] skin has been confirmed to have a variety of biological activities, but its effect on the gut microbiota has not been considered. This study aimed to investigate the effect of hot water extraction of mung bean skin water‐soluble polysaccharides (MB ...
... Specific microorganisms in the human gut (i.e., gut microbes) provide mutually beneficial outcomes such as microbial balance by inhibiting the growth of pathogenic organisms, immune system modulation, fermentation of ingested products, and vitamin production. The intake of contaminants including potenially toxic elements (PTEs) can occur through food, air, water and some medicines. The gut microbe ...
... Brachyspira (B.) pilosicoli is a bacterium causing porcine intestinal spirochaetosis, a disease characterized by diarrhoea and depressed growth rates especially in nursery and fattening pigs. Knowledge of the epidemiology and antimicrobial susceptibility of this pathogen is limited. Therefore, the aim of this study was to analyse the distribution, genetic heterogeneity, and antimicrobial susceptib ...
... From a unicellular bacterium to a more complex human, smell and taste form an integral part of the basic sensory system. In fruit flies Drosophila melanogaster, the behavioral responses to odorants and tastants are simple, though quite sensitive, and robust. They explain the organization and elementary functioning of the chemosensory system. Molecular and functional analyses of the receptors and o ...
... Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course. Its etiology is closely related to abnormal interactions between host and gut microbiota. The mucus barrier lining the gastrointestinal tract is necessary to coordinate host and gut microbiota interaction by nourishing and modulating the microbiota. Differential e ...
... As a potential eco-friendly and sustainable method of ground improvement, microbiologically induced carbonate precipitation (MICP) has drawn considerable attention from researchers over the last few years. In this study, Bacillus megaterium, a nonpathogenic microorganism has been used for MICP treatment to enhance the shear strength of the weak silty soil. In the 1st stage of the experiment, bacte ...
Bordetella pertussis; adhesins; atomic force microscopy; bacteria; cell walls; coevolution; cough; hemagglutinins; modulus of elasticity; spectroscopy; virulence
Abstract:
... In recent years, the coevolution of microorganisms with current antibiotics has increased the mechanisms of bacterial resistance, generating a major health problem worldwide. Bordetella pertussis is a bacterium that causes whooping cough and is capable of adopting different states of virulence, i.e. virulent or avirulent states. In this study, we explored the nanomechanical properties of both viru ...
... Chain elongation is an anaerobic biotechnological process that converts short chain carboxylates and an electron donor (e.g. ethanol, lactate) into more valuable medium chain carboxylates. Caproate production in lactate-based chain elongation is gaining popularity, however, the relation between lactate (electron donor) and acetate (electron acceptor) has not yet been fully elucidated. Herein, for ...
... The purpose of this study was to investigate sublethal concentrations (SLC) of bactericidal antibiotics (ampicillin, gentamicin, kanamycin, and vancomycin) on the mutation frequency and stress response of antibiotic-induced-mutated (AIM) Listeria monocytogenes. Three L. monocytogenes strains (reference, clinical, and food isolate strains) were used in this study. SLC of bactericidal antibiotics si ...
... The transgenic soybean expressing the cry1Ac insecticidal gene of the Bacillus thuringiensis bacterium has been widely adopted in agriculture. This bacterium acts as an efficient control agent of pest insects due to the expression of different proteins with entomopathogenic activity such as Cry and Vip. This study investigates the toxicity and mode of interaction between the Cry1Ab, Cry1Ac, Cry2Aa ...
... Organochlorine pesticides, especially lindane and endosulfan, have been demonstrated to be both biodegradable and frequently coexistent, but their inhibitory effect has never been studied. In this study, we investigated the effect of endosulfan coexistence on lindane degradation to a lindane-degrading isolate, Novosphingobium barchaimii strain LL02, and mixed enrichment cultures from two different ...
Bacillus cereus; activated sludge; bacteria; bioremediation; decolorization; laccase; lignin; manganese; pulp and paper mill effluents; pulp and paper mills; toxicity; wastewater
Abstract:
... The effective degradation of KL from paper mill effluent is an important for environmental safety. This research is primarily concerned with the identification of KL-degrading Bacillus cereus from activated sludge and their possible use for the degradation of Kraft lignin (KL). This strain was involved in the production of lignin peroxidase-LiP (3.20 U/mL), manganese peroxidase-MnP (20.36 U/mL), a ...
... To investigate the potential of Ampelopsis grossedentata extract used as a feed additive, laying performance, egg quality, yolk cholesterol, plasma biochemical parameters, intestinal histology, and gut microbiota of hens (n = 60) were determined between basal diet (CK) and dietary supplementation with A. grossedentata extract (RT) for 11 weeks. The laying rate in RT group was 6.3 percentage points ...
Acidobacteria; Actinobacteria; Chloroflexi; Cyanobacteria; Firmicutes; Myxococcus; bacterial communities; cadmium; community structure; copper; heavy metals; lead; pH; pollution; potassium; soil fertility; soil microorganisms; soil remediation; total nitrogen; zinc; China
Abstract:
... Heavy metal (HM) pollution is a severe and common environmental problem in mining area soil. It is imperative to understand the micro ecological characteristics of mining area soil for HM contaminated soil remediation. This study described the effects of HM pollution level and soil physical and chemical parameters on microbial diversity. In this study, high-throughput sequencing technology was use ...
... Byur dMar Nyer lNga Ril Bu (BdNlRB) is a classic Tibetan medicine prescription for treating " white vein disease". Alzheimer's disease (AD) is a chronic degenerative disease of the central nervous system, characterized by distinct "white vein disease". In the absence of effective drugs for AD, BdNlRB may be a possible treatment for AD.To verify the therapeutic effect and possible mechanism of the ...
... In light of spreading antibiotic resistance among pathogenic bacteria, the development of novel approaches to combat such microorganisms is crucial. Salmonella enterica is pathogenic to humans, however, it can also infect poultry, being a potential foodborne pathogen when poultry-derived food is contaminated by this bacterium. Phage therapy is one of the alternative ways to treat Salmonella-infect ...
... Lactobionic acid (LBA) is an emerging chemical that has been widely utilized in food, cosmetic, and pharmaceutical industries. We sought to produce LBA using Escherichia coli. LBA can be produced from lactose in E. coli, which is innately unable to produce LBA, by coexpressing a heterologous quinoprotein glucose dehydrogenase (GDH) and a pyrroloquinoline quinone (PQQ) synthesis gene cluster. Using ...
... The cocultivation of Clostridium acetobutylicum and Mucor indicus was evaluated for the first time to take the advantages of both microorganisms for fully exploiting the energy potential of the organic fraction of municipal solid waste (OFMSW), generating high yields of acetone-butanol-ethanol (ABE) and hydrogen through a biorefinery scheme. Unlike M. indicus, C. acetobutylicum can ferment unhydro ...
... A new bacterium, Rhodococcus sp. S2-17, which could completely degrade an emerging organic pollutant, benzophenone-3 (BP-3), was isolated from contaminated sediment through an enrichment procedure, and its BP-3 catabolic pathway and genes were identified through metabolic intermediate and transcriptomic analyses and biochemical and genetic studies. Metabolic intermediate analysis suggested that st ...
... Bacteria survived and thrived inside any part of plant without damaging its host are called endophytic bacteria. Many studies have focused on plant–endophytic bacterium interactions and their Plant-growth-promoting (PGP) abilities. Albino tea plants are widely investigated because of their high amino acid concentrations. Previous studies mainly elucidated specific metabolite mechanisms in albino t ...
... A common way for bacteria to cooperate is via the secretion of beneficial public goods (proteases, siderophores, biosurfactants) that can be shared amongst individuals in a group. Bacteria often simultaneously deploy multiple public goods with complementary functions. This raises the question whether natural selection could favour division of labour where subpopulations or species specialize in th ...
... Wolbachia is an endosymbiotic bacterium found in many species of arthropods and manipulates its host reproduction. Cytoplasmic incompatibility (CI) is one of the most common manipulations that is induced when an uninfected female mates with a Wolbachia-infected male. The CI factors (cifA and cifB genes) are encoded by phage WO that naturally infects Wolbachia. Here, we questioned whether an enviro ...
Vibrio parahaemolyticus; Washington (state); bacteria; economies of scale; farms; gastrointestinal diseases; labor; markets; oyster culture; oysters; raw seafoods; research; risk; shellfish; stakeholders; surveys
Abstract:
... The bacterium Vibrio parahaemolyticus (Vp) causes gastrointestinal illness in people, generally through the consumption of undercooked or raw seafood. It is a major concern in the oyster industry, especially as the raw oyster market gains in popularity. As a result, a network of adaptive regulations is triggered during times when Vp risk is high (i.e., during warmer temperatures) and reporting req ...
... A novel, integrated process for the production of ethanol from lignocellulosic biomass is reported which involves simultaneous pretreatment, saccharification and fermentation using environmentally friendly technologies. The lignocellulosic biomass (8.33 g/L rice straw) was pretreated and saccharified with the anaerobic fungus Pecoramyces sp. F1, while the facultative anaerobic bacterium Zymomonas ...
Acidithiobacillus ferrooxidans; active sites; aspartic acid; bacteria; electron transfer; electron transport chain; energy; glutamic acid; histidine; ligands; molecular dynamics; mutants; mutation; oxidation; redox potential
Abstract:
... Cyc₁ (Cytochrome c₅₅₂) is a protein in the electron transport chain of the Acidithiobacillus ferrooxidans (Af) bacteria which obtain their energy from oxidation Fe²⁺ to Fe³⁺. The electrons are directed through Cyc₂, RCY (rusticyanin), Cyc₁ and Cox aa₃ proteins to O₂. Cyc₁ protein consists of two chains, A and B. In the present study, a novel mutation (E121D) in the A chain of Cyc₁ protein was sele ...