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You searched for: Author "Nakayasu, Chihaya" Remove constraint Author: "Nakayasu, Chihaya" Subject bacterial infections Remove constraint Subject: bacterial infections Subject Edwardsiella tarda Remove constraint Subject: Edwardsiella tarda Subject Paralichthys olivaceus Remove constraint Subject: Paralichthys olivaceus
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1. Suppression subtractive hybridization coupled with microarray analysis to examine differential expression of genes in Japanese flounder Paralichthys olivaceus leucocytes during Edwardsiella tarda and viral hemorrhagic septicemia virus infection

Author:
Nakayasu, Chihaya,   et al.   ; Matsuyama, Tomomasa; Fujiwara, Atushi; Takano, Tomokazu;     Show all 4 Authors
Source:
Fish & shellfish immunology 2011 v.31 no.4 pp. 524-532
ISSN:
1050-4648
Subject:
Edwardsiella tarda; Paralichthys olivaceus; Viral hemorrhagic septicemia virus; bacterial infections; cDNA libraries; chemokines; clones; complementary DNA; flounder; gene expression; gene expression regulation; genes; interferons; leukocytes; messenger RNA; microarray technology; pathogens; response elements; suppression subtractive hybridization; transcription (genetics)
Abstract:
... Transcriptional changes in the peripheral blood leucocytes (PBL) of Japanese flounder Paralichthys olivaceus challenged by Edwardsiella tarda and viral hemorrhagic septicemia virus (VHSV) were investigated using suppression subtractive hybridization (SSH) coupled with cDNA microarray analysis. First, we constructed an SSH cDNA library using mRNA samples isolated from PBL of P. olivaceus that had b ...
DOI:
10.1016/j.fsi.2011.06.015

2. Identification of Major Antigenic Proteins of Edwardsiella Tarda Recognized by Japanese Flounder Antibody

Author:
Nakayasu, Chihaya,   et al.   ; Sakai, Takamitsu; Matsuyama, Tomomasa; Nishioka, Toyohiro; Kamaishi, Takashi; Yamaguchi, Kenichi; Iida, Takaji;     Show all 7 Authors
ISSN:
1040-6387
Subject:
bacterial antigens; bacterial proteins; pathogen identification; Edwardsiella tarda; Paralichthys olivaceus; animal pathogenic bacteria; vaccine development; bacterial infections; fish diseases; disease diagnosis; antigen detection; Japan
Abstract:
... Edwardsiella tarda is a fish pathogen that causes systemic infections in fresh water and marine fish. Determining the antigenic proteins is important for the development of an immunodiagnostic tests and a vaccine for effective infection control in fish. In the current study, antigens were detected by immunoblotting and affinity column chromatography using a Japanese flounder (Paralichthys olivaceu ...
DOI:
10.1177/104063870902100412
PubMed:
19564499