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1. Bacteriostatic effects of benzyl isothiocyanate on Vibrio parahaemolyticus: Transcriptomic analysis and morphological verification

Author:
Jianan Liu; Ke Zhang; Jie Song; Hongyan Wu; Hongshun Hao; Jingran Bi; Hongman Hou; Gongliang Zhang
Source:
BMC biotechnology 2021 v.21 no.1 pp. 56
ISSN:
1472-6750
Subject:
Vibrio parahaemolyticus; antibacterial properties; benzyl isothiocyanate; biofilm; biotechnology; estuaries; fish; flagellum; foodborne illness; freshwater; gene expression; gentian violet; minimum inhibitory concentration; quantitative polymerase chain reaction; seafoods; transcriptomics; virulence
Abstract:
... BACKGROUND: Foodborne illness caused by Vibrio parahaemolyticus (V. parahaemolyticus) is generally associated with the consumption of seafood. Fish and other seafood can be contaminated with V. parahaemolyticus, natural inhabitants of the marine, estuarine, and freshwater environment. In this study, the antibacterial activities of benzyl isothiocyanate (BITC) against V. parahaemolyticus were inves ...
DOI:
10.1186/s12896-021-00716-4

2. Biochemical analysis of the initial carcinogenic changes that induce preneoplastic and neoplastic cell populations during chemical hepatocarcinogenesis in rats

Author:
Kimihiko Satoh
Source:
Analytical biochemistry 2022 v.643 pp. 114542
ISSN:
0003-2697
Subject:
DNA microarrays; benzyl isothiocyanate; carcinogenicity; cell division; diet; glutathione transferase; hepatocytes; liver; necrosis; neoplasm cells; protein synthesis; rats
Abstract:
... To analyze the initial carcinogenic changes that induce preneoplastic and neoplastic cell populations in the rat liver, a short-term in vivo promotion assay method was developed. Preneoplastic foci and nodules were quantitated with glutathione S-transferase P-form (GST-P) and γ-glutamyl transpeptidase. Among the four agents tested, benzyl isothiocyanate (BITC) demonstrated the strongest promotor a ...
DOI:
10.1016/j.ab.2021.114542

3. Exploration of the antimicrobial activity of benzyl isothiocyanate against Salmonella enterica serovar Typhimurium

Author:
Hong-Yan Wu; Tong-Xin Niu; Jing-Ran Bi; Hong-Man Hou; Hong-Shun Hao; Gong-Liang Zhang
Source:
Journal of food measurement & characterization 2022 v.16 no.1 pp. 500-506
ISSN:
2193-4126
Subject:
Brassicaceae; Salmonella Typhimurium; anti-infective agents; antibacterial properties; benzyl isothiocyanate; cell membranes; dose response; measurement; minimum inhibitory concentration; reverse transcriptase polymerase chain reaction; virulence
Abstract:
... Mounting evidence suggests that benzyl isothiocyanate (BITC), derived from cruciferous vegetables, could be an effective antimicrobial compound against a wide range of food-borne bacteria. This research investigated the antimicrobial activity of BITC against Salmonella enterica serovar Typhimurium (S. Typhimurium) by determining the minimum inhibitory concentration (MIC) value, observing S. Typhim ...
DOI:
10.1007/s11694-021-01175-3

4. Influences of Dietary Supplementation with Maca (Lepidium meyenii) on Performance, Parameters of Growth Curve and Carcass Characteristics in Japanese Quail

Author:
Firdevs Korkmaz Turgud; Doğan Narinç
Source:
Animals 2022 v.12 no.3 pp. -
ISSN:
2076-2615
Subject:
Coturnix japonica; Lepidium meyenii; antioxidants; benzyl isothiocyanate; chicks; dietary supplements; egg production; feed conversion; feed intake; glucosinolates; growth curves; logit analysis; phenolic compounds; quails; slaughter
Abstract:
... Maca plant contains rich nutrients and in addition, it has various bioactive substances (N-benzyl-palmitamide, benzyl isothiocyanate, glucosinolates and phenolics). It is used to improve reproductive properties and has antioxidant effects for both humans and animals. The aim of this study was to determine the effects of adding maca plant powder to the ration at different levels on growth, slaughte ...
DOI:
10.3390/ani12030318

5. Inhibitory effects of 3-(methylthio) propyl isothiocyanate in comparison with benzyl isothiocyanate on Listeria monocytogenes

Author:
Hong-Yan Wu; Yu-Hang Xu; Li-Na Wei; Jing-Ran Bi; Hong-Man Hou; Hong-Shun Hao; Gong-Liang Zhang
Source:
Journal of food measurement & characterization 2022 v.16 no.2 pp. 1768-1775
ISSN:
2193-4126
Subject:
Listeria monocytogenes; antibacterial properties; benzyl isothiocyanate; biofilm; gene expression; genes; measurement; minimum inhibitory concentration; quantitative polymerase chain reaction
Abstract:
... The objective of the current study was to investigate the inhibitory effects of benzyl isothiocyanate (BITC) and 3-(methylthio) propyl isothiocyanate (MTPITC) on Listeria monocytogenes (L. monocytogenes). The comparison of the antibacterial potential of these two sulfur-containing flavors was performed by examining the minimum inhibitory concentration (MIC). Moreover, the relative gene expression ...
DOI:
10.1007/s11694-022-01290-9

6. Preparation of benzyl isothiocyanate nanoemulsions by different emulsifiers: Stability and bioavailability

Author:
Tian Tian; Xiaohong Tong; Yue Yuan; Bo Lyu; Dengzhen Jiang; Wenyu Cui; Xiaoyi Cheng; Liang Li; Yang Li; Lianzhou Jiang; Huan Wang
Source:
Process biochemistry 2021 v.111 pp. 128-138
ISSN:
1359-5113
Subject:
benzyl isothiocyanate; bioavailability; chitosan; digestibility; digestion; emulsifiers; nanoemulsions; oils; particle size; soy protein isolate; ultraviolet-visible spectroscopy
Abstract:
... The stability and digestibility of food emulsions are affected by the various sources and structural characteristics of emulsifiers. Chitosan, Arabia gum, soy protein isolate (SPI), and soybean protein isolate-phosphatidylcholine (SPI-PC) were used as natural emulsifiers in this study, and a 10 % (v/v) MCT oil phase (containing 10 methyl g/mL benzyl isothiocyanate (BITC)) was used for the preparat ...
DOI:
10.1016/j.procbio.2021.09.001

7. Preparation of chitosan-cellulose-benzyl isothiocyanate nanocomposite film for food packaging applications

Author:
Jinghui Jiang; Xiaoxia Chen; Gong-Liang Zhang; Hongshun Hao; Hong-Man Hou; Jingran Bi
Source:
Carbohydrate polymers 2022 v.285 pp. 119234
ISSN:
0144-8617
Subject:
Escherichia coli; Salmonella Typhimurium; Staphylococcus aureus; antibacterial properties; benzyl isothiocyanate; cellulose; cellulose nanofibers; chickens; chitosan; crosslinking; nanocomposites; nanocrystals; oxygen; plate count; tensile strength; total volatile basic nitrogen
Abstract:
... To improve the mechanical and barrier properties of cellulose and chitosan (CS) and to allow the application of volatile antibacterial benzyl isothiocyanate (BITC) in active packaging, a double-layer nanocomposite film was prepared. Cellulose nanofibers (CNF) were crosslinked with CS via genipin to build the substrate. Quaternized cellulose nanocrystals (QCNC) were synthesized for carrying BITC as ...
DOI:
10.1016/j.carbpol.2022.119234

8. Antibacterial activity and main action pathway of benzyl isothiocyanate extracted from papaya seeds

Author:
Ping Li; Yi‐meng Zhao; Cui Wang; Hua‐ping Zhu
Source:
Journal of food science 2021 v.86 no.1 pp. 169-176
ISSN:
0022-1147
Subject:
Aspergillus niger; Bacillus subtilis; Escherichia coli; Penicillium citrinum; Salmonella enterica; Staphylococcus aureus; antibacterial properties; benzyl isothiocyanate; coenzymes; electron transport chain; food industry; food preservation; fruits; hydrogen; minimum inhibitory concentration; oxidation; papayas; permeability; secondary metabolites
Abstract:
... The development of natural antimicrobial agents has attracted long‐term attention due to the increasing demand for food preservation. Papaya, a widely cultivated nutritious tropical fruit, has benzyl isothiocyanate (BITC) as one of the most important secondary metabolites in its seeds. And the antibacterial activity of BITC toward different strains and the main antibacterial pathway remain unclear ...
DOI:
10.1111/1750-3841.15539

9. Antimicrobial and antihelminthic impacts of black cumin, pawpaw and mustard seeds in livestock production and health

Author:
Moyosore J. Adegbeye; Mona M. M. Y. Elghandour; Tolulope O. Faniyi; Nallely Rivero Perez; Alberto Barbabosa-Pilego; Adrian Zaragoza-Bastida; Abdelfattah Z. M. Salem
Source:
Agroforestry systems 2020 v.94 no.4 pp. 1255-1268
ISSN:
0167-4366
Subject:
Aspergillus; Brassica nigra; Carica papaya; Enterobacter cloacae; Escherichia coli; Nigella sativa; agroforestry; anthelmintics; benzyl isothiocyanate; biofilm; endoparasites; glucosinolates; livestock nutrition; livestock production; methane production; mustard oil; probiotics; production costs; sinapine
Abstract:
... The resistance of microbial strain to the use of medically important antibiotics, high cost of production, and the resistance of ecto and endoparasite to anthelminthic and acaricidal is a cause for concern. There has been intensified effort in search for alternatives to synthetic drugs. Such alternative must be able to kill, reduce, or inhibit pathogenic microbial population while improving the co ...
DOI:
10.1007/s10457-018-0337-0

10. Application and mechanism of benzyl-isothiocyanate, a natural antimicrobial agent from cruciferous vegetables, in controlling postharvest decay of strawberry

Author:
Yaya Sun; Ying Wang; Yong Xu; Tong Chen; Boqiang Li; Zhanquan Zhang; Shiping Tian
Source:
Postharvest biology and technology 2021 v.180 pp. 111604
ISSN:
0925-5214
Subject:
Botrytis cinerea; Brassicaceae; amino acids; anti-infective agents; antineoplastic activity; benzyl isothiocyanate; biosynthesis; carbohydrates; cell death; fruits; gene expression regulation; gray mold; lipids; mechanism of action; plasma membrane; postharvest diseases; postharvest losses; secondary metabolites; spore germination; strawberries; technology; transcriptomics
Abstract:
... Gray mold, caused by Botrytis cinerea, is an important cause of postharvest loss of strawberry fruit. In the present study, we found that benzyl-isothiocyanate (BITC), a natural compound widely existing in cruciferous vegetables with anti-cancer effect, was effective in controlling postharvest gray mold of strawberry fruit, and also strongly inhibited the natural decay of strawberry. The mode of a ...
DOI:
10.1016/j.postharvbio.2021.111604

11. Beneficial Role of Carica papaya Extracts and Phytochemicals on Oxidative Stress and Related Diseases: A Mini Review

Author:
Yew Rong Kong; Yong Xin Jong; Manisha Balakrishnan; Zhui Ken Bok; Janice Kwan Kah Weng; Kai Ching Tay; Bey Hing Goh; Yong Sze Ong; Kok Gan Chan; Learn Han Lee; Kooi Yeong Khaw
Source:
Biology 2021 v.10 no.4 pp. -
ISSN:
2079-7737
Subject:
Carica papaya; alpha-tocopherol; antioxidant activity; benzyl isothiocyanate; caffeic acid; inflammation; kaempferol; myricetin; oxidative stress; papain; quercetin; rutin
Abstract:
... Oxidative stress is a result of disruption in the balance between antioxidants and pro-oxidants in which subsequently impacting on redox signaling, causing cell and tissue damages. It leads to a range of medical conditions including inflammation, skin aging, impaired wound healing, chronic diseases and cancers but these conditions can be managed properly with the aid of antioxidants. This review f ...
DOI:
10.3390/biology10040287

12. Benzyl Isothiocyanate Ameliorates High-Fat Diet-Induced Hyperglycemia by Enhancing Nrf2-Dependent Antioxidant Defense-Mediated IRS-1/AKT/TBC1D1 Signaling and GLUT4 Expression in Skeletal Muscle

Author:
Wei-Ting Chuang; Chih-Ching Yen; Chin-Shiu Huang; Haw-Wen Chen; Chong-Kuei Lii
Source:
Journal of agricultural and food chemistry 2020 v.68 no.51 pp. 15228-15238
ISSN:
1520-5118
Subject:
antioxidant activity; benzyl isothiocyanate; cholesterol; food chemistry; free fatty acids; glucose; high fat diet; hyperglycemia; insulin; insulin resistance; lipotoxicity; muscles; myotubes; obesity; palmitic acid; phosphorylation; skeletal muscle
Abstract:
... Obesity caused lipotoxicity, which results in insulin resistance. We studied whether benzyl isothiocyanate (BITC) improved insulin resistance in muscle. BITC was studied in vivo in mice fed a high-fat diet (HFD) and in vitro in C2C12 myotubes treated with palmitic acid (PA). In C2C12 cells, BITC mitigated PA inhibition of glucose uptake and phosphorylation of IRS-1, AKT, and TBC1D1 in response to ...
DOI:
10.1021/acs.jafc.0c06269

13. Benzyl isothiocyanate fumigation inhibits growth, membrane integrity and mycotoxin production in Alternaria alternata

Author:
Tiaolan Wang; Yongcai Li; Yang Bi; Miao Zhang; Tingting Zhang; Xiaoyuan Zheng; Yupeng Dong; Yi Huang
Source:
RSC advances 2020 v.10 no.3 pp. 1829-1837
ISSN:
2046-2069
Subject:
Alternaria alternata; altenuene; antifungal properties; benzyl isothiocyanate; dose response; electrical conductivity; fumigation; mycelium; pears; plasma membrane; propidium; spore germination; spores; staining
Abstract:
... The antifungal activity of benzyl isothiocyanate (BITC) against pear pathotype-Alternaria alternata, the causal agent of pear black spot, and its possible mechanisms were studied. The results indicated that both the spore germination and mycelial growth of A. alternata were significantly inhibited by BITC in a dose-dependent manner. BITC concentrations at 1.25 mM completely suppressed mycelial gro ...
DOI:
10.1039/c9ra09225k

14. Benzyl isothiocyanate-modified α-lactalbumin – Two-dimensional high-performance thin-layer chromatography for analyzing modified peptides

Author:
Svenja Badekow; Mascha Treblin; Jenny Spöttel; Sascha Rohn
Source:
Journal of chromatography 2021 v.1181 pp. 122937
ISSN:
1570-0232
Subject:
Lepidium sativum; benzyl isothiocyanate; digestibility; hydrolysis; hydrophobicity; metabolites; peptides; proteolysis; thin layer chromatography; trypsin
Abstract:
... In complex food matrices, non-directed reactions between food proteins and secondary plant metabolites (SPM) are conceivable. In this study, the interaction between the bioactive metabolite from garden cress (Lepidium sativum) and selected Brassicaceae – benzyl isothiocyanate (BITC) – and the dairy protein α-lactalbumin (α-LA) was investigated. It was focused on monitoring the proteolytic degradat ...
DOI:
10.1016/j.jchromb.2021.122937

15. Characterization of the Inclusion Complexes of Isothiocyanates with γ-Cyclodextrin for Improvement of Antibacterial Activities against Staphylococcus aureus

Author:
Jianan Liu; Hongyan Wu; Xinying Ao; Hongshun Hao; Jingran Bi; Hongman Hou; Gongliang Zhang
Source:
Foods 2021 v.11 no.1 pp. -
ISSN:
2304-8158
Subject:
Staphylococcus aureus; X-ray diffraction; agar; antibacterial properties; benzyl isothiocyanate; biofilm; breasts; cooked foods; encapsulation; gentian violet; logit analysis; quantitative polymerase chain reaction; thermogravimetry; virulence
Abstract:
... The aim of this study was to develop inclusions formed by γ-cyclodextrin (γ-CD) and three isothiocyanates (ITCs), including benzyl isothiocyanate (BITC), phenethyl isothiocyanate (PEITC), and 3-methylthiopropyl isothiocyanate (MTPITC) to improve their controlled release for the inhibition of Staphylococcus aureus (S. aureus). These inclusion complexes were characterized using X-ray diffraction, Fo ...
DOI:
10.3390/foods11010060

16. Chemical composition, antibacterial and antioxidant activities of oils obtained by different extraction methods from Lepidium sativum L. seeds

Author:
Tokuma Getahun; Vinit Sharma; Neeraj Gupta
Source:
Industrial crops and products 2020 v.156 pp. 112876
ISSN:
0926-6690
Subject:
2,2-diphenyl-1-picrylhydrazyl; Bacillus cereus; Escherichia coli; Klebsiella pneumoniae; Lepidium sativum; Staphylococcus aureus; antibacterial properties; antioxidant activity; bacteria; benzaldehyde; benzyl isothiocyanate; chemical composition; distillation; essential oils; gas chromatography-mass spectrometry; hydrogen peroxide; inhibitory concentration 50; linoleic acid; oils; oleic acid; palmitic acid; petroleum; secondary metabolites; seeds; stearic acid
Abstract:
... The oils obtained from plants by different extraction methods and their constituents are known to possess a vast range of pharmacological and biological activities. The essential oils (EOs) extracted by different methods viz. Clevenger type apparatus (CTA), modified simple distillation (MSD), and simultaneous distillation extraction (SDE) and fatty acid (FA) compositions of the petroleum ether Sox ...
DOI:
10.1016/j.indcrop.2020.112876

17. Comparative metabolome classification of desert truffles Terfezia claveryi and Terfezia boudieri via its aroma and nutrients profile

Author:
Mohamed A. Farag; Doaa Fathi; Samir Shamma; Mohamed Sherif A. Shawkat; Sohir M. Shalabi; Hesham R. El Seedi; Sherif M. Afifi
Source:
Lebensmittel-Wissenschaft + Technologie 2021 v.142 pp. 111046
ISSN:
0023-6438
Subject:
Terfezia; anti-infective agents; benzyl isothiocyanate; diet; ectomycorrhizae; furans; headspace analysis; metabolites; metabolome; monoterpenoids; octenol; odors; solid phase microextraction; sulfur; truffles
Abstract:
... Desert truffles are popular ectomycorrhizal fungi valued worldwide for their nutritional and traditional health benefits. Herein, two chief desert truffles viz., Terfezia claveryi and T. boudieri were assessed for their metabolites heterogeneity in context of their volatile sensory and nutrients profile as analyzed via GC-MS. Primary metabolites accounting for nutritive indices in T. claveryi and ...
DOI:
10.1016/j.lwt.2021.111046

18. Comparison of the inhibitory potential of benzyl isothiocyanate and phenethyl isothiocyanate on Shiga toxin-producing and enterotoxigenic Escherichia coli

Author:
Chun-Xue Yang; Hai-Tao Wu; Xin-Xin Li; Hong-Yan Wu; Tong-Xin Niu; Xiao-Ning Wang; Rui Lian; Gong-Liang Zhang; Hong-Man Hou
Source:
Lebensmittel-Wissenschaft + Technologie 2020 v.118 pp. 108806
ISSN:
0023-6438
Subject:
Shiga toxin-producing Escherichia coli; adenosine triphosphate; antibacterial properties; benzyl isothiocyanate; dose response; enterotoxigenic Escherichia coli; gene expression regulation; genes; minimum inhibitory concentration; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; scanning electron microscopy; toxigenic strains; virulence
Abstract:
... The aim of this study was to investigate the inhibitory effects of benzyl isothiocyanate (BITC) and phenethyl isothiocyanate (PEITC) against Shiga toxin-producing Escherichia coli (STEC) and enterotoxigenic E. coli (ETEC). Compared with that of PEITC, the antibacterial effect of BITC was more obvious. The minimal inhibitory concentration (MIC) of BITC was 60 and 160 μmol/L for STEC and ETEC, while ...
DOI:
10.1016/j.lwt.2019.108806

19. Determination of Isothiocyanate-Protein Conjugates in a Vegetable-Enriched Bread

Author:
Mareike Krell; Lina Cvancar; Michael Poloczek; Franziska S. Hanschen; Sascha Rohn
Source:
Foods 2021 v.10 no.6 pp. -
ISSN:
2304-8158
Subject:
Lepidium sativum; Lewis acids; Tropaeolum majus; benzyl isothiocyanate; bioactive properties; bioavailability; breads; cyanides; dough; food matrix; glucosinolates; health promotion; lysine; migratory behavior; thiourea; traditional foods
Abstract:
... Vegetables of the plant order Brassicales are believed to have health-promoting properties, as they provide high contents of glucosinolates (GLS) and deriving from these, enzymatically and heat-induced breakdown products, such as isothiocyanates (ITC). Besides their positive physiological effects, ITC are electrophilic and can undergo reactions with food components such as proteins. Following the ...
DOI:
10.3390/foods10061300

20. Effects of selenate applied at two growth stages on the nutrient quality of Cardamine violifolia

Author:
Shen Rao; Tian Yu; Xin Cong; Weiwei Zhang; Zhenzhou Zhu; Yongling Liao; Jiabao Ye; Shuiyuan Cheng; Feng Xu
Source:
Scientia horticulturae 2021 v.288 pp. 110352
ISSN:
0304-4238
Subject:
Cardamine; ascorbic acid; benzyl isothiocyanate; carotenoids; chlorophyll; flavonoids; free amino acids; glucosinolates; hydroponics; hyperaccumulators; phenolic acids; selenates; selenium; China
Abstract:
... Cardamine violifolia is an edible selenium (Se) hyperaccumulator that is mainly distributed in Enshi, China. Here, C. violifolia treated with different selenate concentrations (i.e., 0, 1.5, 20, and 80 μmol L ⁻ ¹) at the rosette and podding stages in a hydroponic experiment was carried out to investigate the effects of Se accumulation on the nutrient quality of this crop. The shoots accumulated hi ...
DOI:
10.1016/j.scienta.2021.110352

21. Enhancing the fungicidal activity of amphotericin B via vacuole disruption by benzyl isothiocyanate, a cruciferous plant constituent

Author:
N. Yamada; W. Murata; Y. Yamaguchi; K.‐I. Fujita; A. Ogita; T. Tanaka
Source:
Letters in applied microbiology 2021 v.72 no.4 pp. 390-398
ISSN:
0266-8254
Subject:
Brassicaceae; Candida albicans; Saccharomyces cerevisiae; amphotericin B; antifungal properties; benzyl isothiocyanate; cell viability; drug therapy; ergosterol; membrane permeability; mycoses; plasma membrane; vacuoles; virulent strains; yeasts
Abstract:
... Amphotericin B (AmB), a typical polyene macrolide antifungal agent, is widely used to treat systemic mycoses. In the present study, we show that the fungicidal activity of AmB was enhanced by benzyl isothiocyanate (BITC), a cruciferous plant‐derived compound, in the budding yeast, Saccharomyces cerevisiae. In addition to forming a molecular complex with ergosterol present in fungal cell membranes ...
DOI:
10.1111/lam.13425

22. Inhibitory effect of allyl and benzyl isothiocyanates on ochratoxin a producing fungi in grape and maize

Author:
Bolei Yang; Li Li; Hairong Geng; Chenxi Zhang; Gang Wang; Shuo Yang; Sheng Gao; Yueju Zhao; Fuguo Xing
Source:
Food microbiology 2021 v.100 pp. 103865
ISSN:
0740-0020
Subject:
Aspergillus ochraceus; allyl isothiocyanate; benzyl isothiocyanate; biosynthesis; corn; food microbiology; fungal growth; genes; grapes; mycelium; ochratoxin A; shelf life; spore germination
Abstract:
... The purpose of this study was to evaluate the inhibitory effect of allyl-isothiocyanate (AITC) and benzyl-isothiocyanate (BITC) on fungal growth and Ochratoxin A (OTA) production by Aspergillus ochraceus, A. carbonarius and A. niger. Here, we found that spore germination and fungal growth of the three fungi were significantly inhibited when the concentration of AITC and BITC was higher than 1.25 μ ...
DOI:
10.1016/j.fm.2021.103865

23. Key Odorants of Raw and Cooked Green Kohlrabi (Brassica oleracea var. gongylodes L.)

Author:
Monika Marcinkowska; Stephanie Frank; Martin Steinhaus; Henryk H. Jeleń
Source:
Journal of agricultural and food chemistry 2021 v.69 no.41 pp. 12270-12277
ISSN:
1520-5118
Subject:
Brassica oleracea var. gongylodes; benzyl isothiocyanate; butanes; disulfides; flavor; food chemistry; kohlrabi; lipid peroxidation; odor compounds; odors; propane; smell; sulfur
Abstract:
... Volatile compounds of raw and cooked green kohlrabi were investigated using a sensomics approach. A total of 55 odor-active compounds were detected and identified in raw and cooked green kohlrabi using GC-O. Twenty-eight odor-active compounds with high flavor dilution (FD) factors ranging from 64 to 1024 were quantitated, and odor activity values (OAVs) were determined. Eight compounds showed high ...
DOI:
10.1021/acs.jafc.1c04339

24. Small Functional Foods: Comparative Phytochemical and Nutritional Analyses of Five Microgreens of the Brassicaceae Family

Author:
Ilaria Marchioni; Marco Martinelli; Roberta Ascrizzi; Costanza Gabbrielli; Guido Flamini; Luisa Pistelli; Laura Pistelli
Source:
Foods 2021 v.10 no.2 pp. -
ISSN:
2304-8158
Subject:
Brassica juncea; Brassica oleracea; Eruca vesicaria subsp. sativa; Nasturtium officinale; Raphanus raphanistrum; allyl isothiocyanate; anthocyanins; antioxidant activity; antioxidants; ascorbic acid; benzyl isothiocyanate; broccoli; carotenoids; chlorophyll; diterpenoids; essential oils; functional foods; microgreens; myrcene; nutrient content; nutritive value; polyphenols; watercress
Abstract:
... Microgreens are the seedlings of herbs and vegetables which are harvested at the development stage of their two cotyledonary leaves, or sometimes at the emergence of their rudimentary first pair of true leaves. They are functional foods, the consumption of which is steadily increasing due to their high nutritional value. The species of the Brassicaceae family are good sources of bioactive compound ...
DOI:
10.3390/foods10020427

25. Transcriptome Analysis and Weighted Gene Co-expression Network Reveal Multitarget-Directed Antibacterial Mechanisms of Benzyl Isothiocyanate against Staphylococcus aureus

Author:
Hongbo Li; Xujia Ming; Dan Xu; Haizhen Mo; Zhenbin Liu; Liangbin Hu; Xiaohui Zhou
Source:
Journal of agricultural and food chemistry 2021 v.69 no.39 pp. 11733-11741
ISSN:
1520-5118
Subject:
RNA; arginine; benzyl isothiocyanate; biosynthesis; death; drugs; flow cytometry; food chemistry; food safety; genes; methicillin-resistant Staphylococcus aureus; minimum inhibitory concentration; oxidative phosphorylation; shrinkage; transcription (genetics); transcriptomics
Abstract:
... Staphylococcus aureus can cause many diseases and has a strong tendency to develop resistance to multiple antibiotics. In this study, benzyl isothiocyanate (BITC) was shown to have an excellent inhibitory effect on S. aureus ATCC25923 and methicillin-resistant S. aureus strains, with a minimum inhibitory concentration of 10 μg/mL. Under a scanning electron microscope, shrinkage and lysis of the ce ...
DOI:
10.1021/acs.jafc.1c03979

26. A new aliphatic ester of hydroxysalicylic acid from fermented Carica papaya L. preparation with a potential hair growth stimulating activity

Author:
Ahmed Ashour; Yhiya Amen; Toshinori Nakagawa; Yasuharu Niwa; Amira Mira; Koichiro Ohnuki; Shinki Murakami; Mitsuko Imao; Kuniyoshi Shimizu
Source:
Natural product research 2020 v.34 no.12 pp. 1750-1755
ISSN:
1478-6427
Subject:
4-hydroxybenzoic acid; Carica papaya; antioxidant activity; benzyl isothiocyanate; commercialization; fruits; functional foods; hydroxymethylfurfural; lycopene; papayas
Abstract:
... An aliphatic ester of hydroxysalicylic acid (6), reported for the first time from a natural source in addition to five known compounds were isolated from the fermented Carica papaya L. preparation, a commercialized functional food. The known compounds were identified as 5-hydroxymethylfurfuraldehyde (1), trans-caffeic acid (2), butyl 4-hydroxybenzoate (butylparaben) (3), lycopene (4), benzyl isoth ...
DOI:
10.1080/14786419.2018.1530231

27. Benzyl isothiocyanate promotes miR‐99a expression through ERK/AP‐1‐dependent pathway in bladder cancer cells

Author:
Te‐Fu Tsai; Po‐Chun Chen; Yi‐Chia Lin; Kuang‐Yu Chou; Hung‐En Chen; Chao‐Yen Ho; Ji‐Fan Lin; Thomas I‐S. Hwang
Source:
Environmental toxicology 2020 v.35 no.1 pp. 47-54
ISSN:
1520-4081
Subject:
Brassicaceae; antineoplastic activity; benzyl isothiocyanate; cell lines; enzyme activity; fibroblast growth factor receptor 3; fluorescent antibody technique; luciferase; mitogen-activated protein kinase; neoplasm cells; phosphorylation; signal transduction; transcription factors; transcriptional activation; urinary bladder neoplasms
Abstract:
... Benzyl isothiocyanate (BITC), a bioactive natural product present in cruciferous vegetables, has been proved to prevent cancer progression through various mechanisms. In our previous report, we proved that BITC exhibits antitumor effects in bladder cancer by suppressing IGF1R, FGFR3, and mTOR, which is mediated by miR‐99a expression. In this study, we identified the signal pathway involved in regu ...
DOI:
10.1002/tox.22841

28. Characterization of benzyl isothiocyanate extracted from mashed green papaya by distillation

Author:
Toshiyuki Nakamura; Yoshiyuki Murata; Yoshimasa Nakamura
Source:
Food chemistry 2019 v.299 pp. 125118
ISSN:
0308-8146
Subject:
aqueous solutions; benzyl isothiocyanate; cysteine; distillation; frozen storage; glutathione; solvents; storage temperature
Abstract:
... The aim of this study was to extract benzyl isothiocyanate (BITC) from green papaya by distillation apparatus without using organic solvents, and to improve the stability of BITC in aqueous solution. The distillation of mashed green papaya successfully yielded BITC as a water solution with more than 80% purity with good reproducibility. The amount of BITC in the distilled water gradually decreased ...
DOI:
10.1016/j.foodchem.2019.125118

29. Cysteine specific bioconjugation with benzyl isothiocyanates

Author:
László Petri; Péter A. Szijj; Ádám Kelemen; Tímea Imre; Ágnes Gömöry; Maximillian T. W. Lee; Krisztina Hegedűs; Péter Ábrányi-Balogh; Vijay Chudasama; György Miklós Keserű
Source:
RSC advances 2020 v.10 no.25 pp. 14928-14936
ISSN:
2046-2069
Subject:
antibodies; benzyl isothiocyanate; cysteine; diagnostic techniques; disulfide bonds; fluorescein; pH
Abstract:
... Protein labelling has a wide variety of applications in medicinal chemistry and chemical biology. In addition to covalent inhibition, specific labelling of biomolecules with fluorescent dyes is important in both target discovery, validation and diagnostics. Our research was conducted through the fragment-based development of a new benzyl-isothiocyanate-activated fluorescent dye based on the fluore ...
DOI:
10.1039/d0ra02934c

30. Effect of benzyl isothiocyanate encapsulated biocompatible nanoemulsion prepared via ultrasonication on microbial strains and breast cancer cell line MDA MB 231

Author:
Shivani Uppal; Pratibha Sharma; Rajendra Kumar; Khushwinder Kaur; Alka Bhatia; S.K. Mehta
Source:
Colloids and surfaces 2020 v.596 pp. 124732
ISSN:
0927-7757
Subject:
Brassicaceae; Escherichia coli; Fourier transform infrared spectroscopy; GRAS substances; Staphylococcus aureus; antimicrobial properties; benzyl isothiocyanate; bioavailability; biocompatibility; breast neoplasms; cell lines; cytotoxicity; dietary supplements; encapsulation; flow cytometry; hemolysis; hydrophobicity; ingredients; nanocarriers; nanoemulsions; neoplasm cells; odors; pH; rhamnolipids; toxicity testing; transmission electron microscopy; ultrasonic treatment; ultraviolet-visible spectroscopy
Abstract:
... Benzyl Isothiocyanate (BITC), a nutraceutical belonging to the mustard family exhibits excellent antitumor and antimicrobial properties. Despite its prodigious potential, the lacuna in the practical application is due to hydrophobicity, high volatility, receded bioavailability, and acute odor. Owing to the entrenched advantages of the nanostructured colloidal systems, a new rhamnolipid based nanoe ...
DOI:
10.1016/j.colsurfa.2020.124732

31. Effect of quorum sensing and quorum sensing inhibitors on the expression of serine protease gene in Hafnia alvei H4

Author:
Yaolei Zhu; Xue Sang; Xue Li; Yanan Zhang; Hongshun Hao; Jingran Bi; Gongliang Zhang; Hongman Hou
Source:
Applied microbiology and biotechnology 2020 v.104 no.17 pp. 7457-7465
ISSN:
0175-7598
Subject:
Hafnia alvei; benzyl isothiocyanate; biotechnology; food spoilage; genes; lactones; serine proteinases; temperature
Abstract:
... The serp gene codes for a protease that is considered to be an important factor associated with quorum sensing (QS)–based food spoilage caused by microorganisms. In this study, we evaluated the effect of temperature (4–37 °C) and QS inhibitors on the production of N-acyl-L-homoserine lactones (AHLs) and relative expression of the luxR/I, as well as serp in Hafnia alvei H4. Production of AHLs and e ...
DOI:
10.1007/s00253-020-10730-9

32. Efficacy of benzyl isothiocyanate for controlling Salmonella on alfalfa seeds and sprouts

Author:
Hsin‐Bai Yin; Ashley Boomer; Chi‐Hung Chen; Jitendra Patel
Source:
International journal of food science & technology 2020 v.55 no.6 pp. 2669-2676
ISSN:
0950-5423
Subject:
Brassicaceae; Medicago sativa; Salmonella enterica; alfalfa; benzyl isothiocyanate; chlorine; inoculum; seed treatment; seeds; serotypes; sprouting; sprouts (food)
Abstract:
... The efficacy of benzyl isothiocyanate (BIT), a compound found in cruciferous vegetables, for controlling Salmonella on alfalfa seeds and sprouts was investigated. Alfalfa seeds were inoculated by individual Salmonella enterica serotypes Newport, Typhimurium, Tennessee, Montevideo, or Braenderup at two inoculation levels of 3 or 5 log CFU g⁻¹, followed by treatment with BIT (0.5–2%) or chlorine (2% ...
Handle:
10113/6943421
DOI:
10.1111/ijfs.14520

33. Fish skin gelatin-based emulsion as a delivery system to protect lipophilic bioactive compounds during in vitro and in vivo digestion: The case of benzyl isothiocyanate

Author:
Yue Tang; Xiaohan Wang; Jiahui Yu; Liang Song; Songyi Lin
Source:
Lebensmittel-Wissenschaft + Technologie 2020 v.134 pp. 110145
ISSN:
0023-6438
Subject:
absorption; atomic force microscopy; benzyl isothiocyanate; bioavailability; digestion; digestive tract; electron microscopy; emulsifiers; emulsions; fish skin; gelatin; intestines; lipid metabolism; lipophilicity; models; permeability; stomach
Abstract:
... To improve the stability of nutrients in the gastrointestinal tract, fish skin gelatin (FSG) was used as an emulsifier and benzyl isothiocyanate (BITC) as a model of nutrient delivery to construct a stable emulsion. Tween-based emulsions were used as controls. The emulsion stability, lipid digestion, and BITC bioavailability were investigated using a simulated gastrointestinal tract model. The in ...
DOI:
10.1016/j.lwt.2020.110145

34. Inhibiting plasmid mobility: The effect of isothiocyanates on bacterial conjugation

Author:
Awo Afi Kwapong; Paul Stapleton; Simon Gibbons
Source:
International journal of antimicrobial agents 2019 v.53 no.5 pp. 629-636
ISSN:
0924-8579
Subject:
Escherichia coli; allyl isothiocyanate; anti-infective agents; antibiotic resistance genes; benzyl isothiocyanate; genetic conjugation; host range; microorganisms; multiple drug resistance; plasmids
Abstract:
... Bacterial conjugation is the main mechanism for the transfer of multiple antimicrobial resistance genes among pathogenic micro-organisms. This process may be controlled by compounds that inhibit bacterial conjugation. In this study, the effects of allyl isothiocyanate, l-sulforaphane, benzyl isothiocyanate, phenylethyl isothiocyanate and 4-methoxyphenyl isothiocyanate on the conjugation of broad-h ...
DOI:
10.1016/j.ijantimicag.2019.01.011

35. Modelling the combined effect of chlorine, benzyl isothiocyanate, exposure time and cut size on the reduction of Salmonella in fresh-cut lettuce during washing process

Author:
Sofia Griselda Cuggino; Isabel Bascón Villegas; Francisco Rincón; Alejandra Pérez Agostini; Guiomar Posada-Izquierdo; Javier Marugán; Cristina Pablos Carro; Fernando Pérez-Rodríguez
Source:
Food microbiology 2019 pp. -
ISSN:
0740-0020
Subject:
Salmonella; benzyl isothiocyanate; chlorine; cutting; disinfectants; disinfection; exposure duration; food safety; fresh-cut produce; lettuce; sodium hypochlorite; statistical analysis; vegetable industry; washing
Abstract:
... This work aimed to study the effect of the combination of Sodium hypochlorite, the most used disinfectant by the vegetable industry, with a natural antimicrobial, benzyl-isothiocyanate (BITC), considering cutting surface and contact time, on the reduction of Salmonella in fresh-cut produce in washing operations under typical industrial conditions. Overall, the combinations of disinfectant and proc ...
DOI:
10.1016/j.fm.2019.103346

36. Moringin and Its Structural Analogues as Slow H₂S Donors: Their Mechanisms and Bioactivity

Author:
Yuyun Lu; Xingyi Wang; Haoliang Pu; Yi Lin; Dan Li; Shao Quan Liu; Dejian Huang
Source:
Journal of agricultural and food chemistry 2020 v.68 no.27 pp. 7235-7245
ISSN:
1520-5118
Subject:
anti-inflammatory activity; anti-inflammatory agents; antibiotics; benzyl isothiocyanate; bioactive compounds; cyclization reactions; cysteine; cytotoxicity; food chemistry; healthy diet; hydrogen sulfide; metabolites
Abstract:
... Moringin (rhamnobenzyl isothiocyanate) is a major bioactive compound in moringa seeds, which have been used as a healthy food. However, its bioactivity mechanisms are not well understood. We investigated moringin and its structurally similar analogues, including benzyl isothiocyanate and 4-hydroxylbenzyl isothiocyanate, for their hydrogen sulfide (H₂S)-releasing activity triggered by cysteine. The ...
DOI:
10.1021/acs.jafc.0c02358

37. Pseudosciaena crocea roe protein‐stabilized emulsions for oral delivery systems: In vitro digestion and in situ intestinal perfusion study

Author:
Yue Tang; Xiaohan Wang; Hui Jiang; Liang Song; Haozhe Cui; Zhihui Zhang; Songyi Lin
Source:
Journal of food science 2020 v.85 no.9 pp. 2923-2932
ISSN:
0022-1147
Subject:
Larimichthys crocea; absorption; benzyl isothiocyanate; bioavailability; digestion; digestive tract; emulsions; food industry; food science; free fatty acids; lipid metabolism; membrane permeability; models; oils; protective effect; protein isolates; small intestine
Abstract:
... Benzyl isothiocyanate (BITC) was encapsulated in oil‐in‐water emulsions stabilized by Pseudosciaena crocea roe protein isolate (PRPI). The stability, lipid digestion, BITC bioavailability, and retention rate of the emulsions were characterized using a simulated gastrointestinal tract model. Tween‐corn and PRPI‐medium‐chain triglycerides (MCT) emulsions were used as controls. The membrane permeabil ...
DOI:
10.1111/1750-3841.15371

38. Structural and energetic investigation on the host/guest inclusion process of benzyl isothiocyanate into β-cyclodextrin using dispersion-corrected DFT calculations

Author:
Abdelaziz Bouhadiba; Seyfeddine Rahali; Youghourta Belhocine; Hamza Allal; Leila Nouar; Mohamed Rahim
Source:
Carbohydrate research 2020 v.491 pp. 107980
ISSN:
0008-6215
Subject:
benzyl isothiocyanate; beta-cyclodextrin; chemical interactions; computer simulation; density functional theory; energy; models; moieties; nuclear magnetic resonance spectroscopy; van der Waals forces
Abstract:
... The formation of host-guest complex between benzyl isothiocyanate (BITC) and β-cyclodextrin (β-CD) was studied using dispersion-corrected density functional theory calculations. The complexation process was monitored using molecular docking simulations, natural bond orbital (NBO) technique, nuclear magnetic resonance (¹H NMR) chemical shift calculations and non-covalent interactions (NCI) analysis ...
DOI:
10.1016/j.carres.2020.107980

39. Suppression of multiple processes relevant to cancer progression by benzyl isothiocyanate may result from the inhibition of Aurora A kinase activity

Author:
Tzu-Tung Yu; Meng-Ya Chang; Yi-Jen Hsieh; Chih-Jui Chang
Source:
Food & function 2020 v.11 no.10 pp. 9010-9019
ISSN:
2042-650X
Subject:
Brassicaceae; angiogenesis; antimitotic agents; antineoplastic activity; apoptosis; benzyl isothiocyanate; carcinogenesis; cell cycle checkpoints; cells; fluorescent antibody technique; glucosinolates; hydrolysis; inactivation; incidence; mitosis; neoplasm progression; non-specific serine/threonine protein kinase; phenotype; phytochemicals; staining; vegetables
Abstract:
... Cruciferous vegetables are good sources of phytochemicals that have the potential to prevent cancer. Benzyl isothiocyanate (BITC) is the hydrolysis product of glucosinolates that are especially abundant in cruciferous vegetables. The anti-cancer activities of BITC have been studied for decades. The mechanisms of reducing the incidence of cancer by BITC involve multiple pathways, including the inhi ...
DOI:
10.1039/d0fo01565b

40. The effect of benzyl isothiocyanate on Candida albicans growth, cell size, morphogenesis, and ultrastructure

Author:
Cheila Pereira; Ana Margarida Calado; Ana Cristina Sampaio
Source:
World journal of microbiology & biotechnology 2020 v.36 no.10 pp. 153
ISSN:
0959-3993
Subject:
Candida albicans; allyl isothiocyanate; antifungal properties; benzyl isothiocyanate; biotechnology; cell viability; cell walls; disk diffusion antimicrobial test; germ tube; morphogenesis; oxidative stress; ultrastructure; yeasts
Abstract:
... Candida albicans is a commensal yeast that may become pathogenic and even lethal to the host. Over the last few decades, antifungal resistance has increased, promoting screening of the antifungal potential of old and new substances. This study investigates the antifungal potential of isothiocyanates (ITCs) against C. albicans oral isolates. A preliminary susceptibility disk diffusion test (DD) was ...
DOI:
10.1007/s11274-020-02929-9

41. Benzyl Isothiocyanate Reduces High-Fat/High-Cholesterol/Cholic Acid Diet-Induced Non-Alcoholic Steatohepatitis by Inhibiting the Activation of NLRP3 Inflammasome (P06-084-19)

Author:
Li-Li Ko; Haw-wen Chen; Chong-Kuei Lii
Source:
Current developments in nutrition 2019 v.3 no.Supplement_1 pp. -
ISSN:
2475-2991
Subject:
Brassicaceae; antineoplastic activity; antioxidants; benzyl isothiocyanate; blood sampling; blood serum; caspase-1; cholesterol; cholic acid; diet; fatty liver; fibrosis; histology; inflammasomes; interleukin-1beta; liver; macrophages; males; messenger RNA; mice; obesity; tissues; triacylglycerols; vegetables; Taiwan
Abstract:
... Non-alcoholic steatohepatitis (NASH) is one kind of fatty liver disease, which is associated with obesity. Benzyl isothiocyanate (BITC), an organosulfur compound enriched in cruciferous vegetables, shows anti-inflammatory, antioxidant, and anti-tumor activities. This study examined whether BITC protects against high-fat/high-cholesterol/cholic acid diet-induced NASH and the underlying mechanisms i ...
DOI:
10.1093/cdn/nzz031.P06-084-19

42. Benzyl Isothiocyanate and Phenethyl Isothiocyanate Inhibit Adipogenesis and Hepatosteatosis in Mice with Obesity Induced by a High-Fat Diet

Author:
Wei-Ting Chuang; Yun-Ta Liu; Chin-Shiu Huang; Chia-Wen Lo; Hsien-Tsung Yao; Haw-Wen Chen; Chong-Kuei Lii
Source:
Journal of agricultural and food chemistry 2019 v.67 no.25 pp. 7136-7146
ISSN:
1520-5118
Subject:
Brassicaceae; acetyl-CoA carboxylase; adipocytes; adipogenesis; benzyl isothiocyanate; cholesterol; dose response; epididymis; fatty liver; fatty-acid synthase; gene expression regulation; high fat diet; immunoblotting; interphase; liver; low fat diet; mice; obesity; oral administration; phytochemicals; stearoyl-CoA desaturase; tissues; transcription factors; triacylglycerols; vegetables
Abstract:
... Benzyl isothiocyanate (BITC) and phenethyl isothiocyanate (PEITC) are organosulfur phytochemicals rich in cruciferous vegetables. We investigated the antiobesity and antihepatosteatosis activities of BITC and PEITC and the working mechanisms involved. C57BL/6J mice were fed a low-fat diet (LFD), a high-fat diet (HFD), or a HFD supplemented with 0.5 (L) or 1 g/kg (H) BITC or PEITC for 18 weeks. Com ...
DOI:
10.1021/acs.jafc.9b02668

43. Chitosan nanoparticles as a biocompatible and efficient nanowagon for benzyl isothiocyanate

Author:
Shivani Uppal; Khushwinder Kaur; Rajendra Kumar; Naval Deep Kaur; Geeta Shukla; S.K. Mehta
Source:
International journal of biological macromolecules 2018 v.115 pp. 18-28
ISSN:
0141-8130
Subject:
X-ray diffraction; antimicrobial properties; benzyl isothiocyanate; bioavailability; biodegradability; biosafety; chitosan; encapsulation; hemolysis; infrared spectroscopy; nanoparticles; polymers; scanning electron microscopy; solubility; sulfur; therapeutics; transmission electron microscopy; ultraviolet radiation; ultraviolet-visible spectroscopy
Abstract:
... A plethora of evidences support the health benefits of a sulfur containing compound called Benzyl Isothiocyanate. However, its therapeutic application is limited due to its low solubility, poor stability and inadequate bioavailability. The problem has been worked upon and resolved by the synthesis of biodegradable nanoparticles using chitosan as the controlled delivery nanowagon. The prepared nano ...
DOI:
10.1016/j.ijbiomac.2018.04.036

44. Effect of isothiocyanates, BITC and PEITC, on stress protein accumulation, protein aggregation and aggresome-like structure formation in Xenopus A6 kidney epithelial cells

Author:
Imran Khamis; John J. Heikkila
Source:
Comparative Biochemistry and Physiology, Part C 2018 v.204 pp. 1-13
ISSN:
1532-0456
Subject:
Lewis acids; Xenopus laevis; actin; benzyl isothiocyanate; epithelial cells; glutathione; heat shock proteins; heme oxygenase (biliverdin-producing); immunocytochemistry; kidneys; microtubules; thiols; tubulin; vegetables
Abstract:
... Numerous studies have elucidated the health benefits of organosulfur compounds, known as isothiocyanates (ITCs), derived from cruciferous vegetables. As electrophiles, ITCs have the ability to directly bind and modify thiol-containing compounds such as glutathione and cellular protein, including tubulin. While the biochemical effects of ITCs have been well characterized, less information is availa ...
DOI:
10.1016/j.cbpc.2017.10.011

45. Fabrication and Physicochemical Characterization of Pseudosciaena crocea Roe Protein‐Stabilized Emulsions as a Nutrient Delivery System

Author:
Yue Tang; Hui Jiang; Haitao Wu; Xiaohan Wang; Yuzhu Chen; Youling L. Xiong; Songyi Lin
Source:
Journal of food science 2019 v.84 no.6 pp. 1346-1352
ISSN:
0022-1147
Subject:
Larimichthys crocea; benzyl isothiocyanate; confocal laser scanning microscopy; emulsifiers; emulsifying properties; emulsions; fish roe; food industry; lipid content; models; nutrients; oils; pH; particle size; protective effect; protein isolates; storage quality; storage temperature; zeta potential
Abstract:
... The aim of this study was to develop a protein emulsifier that can be used to construct a nutrient delivery system. Pseudosciaena crocea roe protein isolate (PRPI) was prepared and tested for its emulsifying properties. Benzyl isothiocyanate (BITC) was used as a model delivery nutrient in the constructed emulsions. The average particle size, zeta potential, and BITC retention rate of the emulsions ...
DOI:
10.1111/1750-3841.14612

46. Health effects and potential mode of action of papaya (Carica papaya L.) bioactive chemicals

Author:
Y. Desjardins
Source:
Acta horticulturae 2019 no.1250 pp. 197-208
ISSN:
0567-7572
Subject:
Carica papaya; abortifacients; anthelmintics; antifertility effect; ascorbic acid; bark; benzyl isothiocyanate; beta-carotene; beta-cryptoxanthin; cardiovascular diseases; dengue; digestive system; dysbiosis; ferulic acid; free radical scavengers; fungi; glucosinolates; health effects assessments; intestinal microorganisms; kaempferol; latex; leaf extracts; leaves; lycopene; malaria; mechanism of action; mesocarp; neoplasms; noninsulin-dependent diabetes mellitus; obesity; p-coumaric acid; papain; papayas; procyanidins; quercetin; roots; seeds; tissue repair; traditional medicine
Abstract:
... Papaya (Carica papaya L.) has been used in traditional medicine to treat many disorders. Leaves, bark, roots, latex, fruits and seeds contain specific phytochemicals that display bioactivity against many diseases. Indeed, experiments have shown that C. papaya possesses anthelmintic, antiprotozoan, antibacterial, antifungal, antiviral, anti-inflammatory, antihypertensive, hypoglycaemic and hypolipi ...
DOI:
10.17660/ActaHortic.2019.1250.28

47. In Vitro Determination of Protein Conjugates in Human Cells by LC-ESI-MS/MS after Benzyl Isothiocyanate Exposure

Author:
Carla Kühn; Tobias von Oesen; Corinna Herz; Monika Schreiner; Franziska S. Hanschen; Evelyn Lamy; Sascha Rohn
Source:
Journal of agricultural and food chemistry 2018 v.66 no.26 pp. 6727-6733
ISSN:
1520-5118
Subject:
benzyl isothiocyanate; bioactive properties; cysteine; digestion; eukaryotic cells; exposure duration; glucosinolates; health promotion; humans; liquid chromatography; lysine; mechanism of action; pH; surveys; tandem mass spectrometry; temperature
Abstract:
... Glucosinolates and their breakdown products, especially isothiocyanates (ITCs), are hypothesized to exert a broad range of bioactivities. However, physiological mechanisms are not yet completely understood. In this study, formation of protein conjugates after incubation with benzyl isothiocyanate (BITC) was investigated in vitro. A survey of protein conjugates was done by determining BITC cysteine ...
DOI:
10.1021/acs.jafc.8b01309

48. Inhibition of Escherichia coli O157:H7 and Salmonella enterica virulence factors by benzyl isothiocyanate

Author:
Jitendra Patel; Hsin-Bai Yin; Gary Bauchan; Joseph Mowery
Source:
Food microbiology 2019 pp. -
ISSN:
0740-0020
Subject:
Escherichia coli O157; Salmonella enterica; Shiga toxin; antimicrobial properties; bacteria; bacterial motility; benzyl isothiocyanate; enzyme-linked immunosorbent assay; food pathogens; glucosinolates; hydrolysis; public health; transmission electron microscopy; virulence; United States
Abstract:
... Escherichia coli O157:H7 and Salmonella enterica are foodborne pathogens with major public health concern in the U.S. These pathogens utilize several virulence factors to initiate infections in humans. The antimicrobial effect of seven glucosinolate hydrolysis compounds against Salmonella and E. coli O157:H7 was investigated by the disc diffusion assay. Among the tested compounds, benzyl isothiocy ...
Handle:
10113/6593914
DOI:
10.1016/j.fm.2019.103303

49. Methyl-β-cyclodextrin potentiates the BITC-induced anti-cancer effect through modulation of the Akt phosphorylation in human colorectal cancer cells

Author:
Qifu Yang; Miku Miyagawa; Xiaoyang Liu; Beiwei Zhu; Shintaro Munemasa; Toshiyuki Nakamura; Yoshiyuki Murata; Yoshimasa Nakamura
Source:
Bioscience, biotechnology, and biochemistry 2018 v.82 no.12 pp. 2158-2167
ISSN:
1347-6947
Subject:
antineoplastic activity; apoptosis; benzyl isothiocyanate; biotechnology; blood serum; calcium; cattle; cholesterol; colorectal neoplasms; cyclodextrins; cytotoxicity; humans; magnesium; mitogen-activated protein kinase; phosphatidylinositol 3-kinase; phosphorylation; plasma membrane; propidium; thin layer chromatography
Abstract:
... Methyl-β-cyclodextrin (MβCD) is an effective agent for the removal of plasma membrane cholesterol. In this study, we investigated the modulating effects of MβCD on the antiproliferation induced by benzyl isothiocyanate (BITC), an ITC compound mainly derived from papaya seeds. We confirmed that MβCD dose-dependently increased the cholesterol level in the medium, possibly through its removal from th ...
DOI:
10.1080/09168451.2018.1514249

50. The potential of Brassicaceae biofumigant crops to manage Pleiochaeta setosa in sustainable lupin cultivation

Author:
Kevin Dewitte; Sofie Landschoot; Jasper Carrette; Kris Audenaert; Veerle Derycke; Joos Latré; Pieter Vermeir; Geert Haesaert
Source:
Biological control 2019 pp. -
ISSN:
1049-9644
Subject:
Lupinus; Pleiochaeta setosa; Raphanus sativus; Sinapis alba subsp. alba; allyl isothiocyanate; antifungal properties; benzyl isothiocyanate; biofumigation; biological control; dimethyl disulfide; disease control; fungal growth; gas chromatography-mass spectrometry; leaves; mycelium; pesticides; radishes; root rot; tissues; vegetative growth
Abstract:
... Lupin is prone to infection by Pleiochaeta setosa (Kirchn.) Hughes causing brown spot and root rot in Lupinus species. The control of these diseases is crucial to limit yield losses, but an increased public awareness and European legislative restrictions have resulted in a drastic reduction of allowed pesticides and many research groups invest in alternative biocontrol strategies. In the current s ...
DOI:
10.1016/j.biocontrol.2019.02.020

51. Aerial parts of maca (Lepidium meyenii Walp.) as functional vegetables with gastrointestinal prokinetic efficacy in vivo

Author:
Wenwen Jin; Xuemin Chen; Qing Huo; Yajie Cui; Zejun Yu; Longjiang Yu
Source:
Food & function 2018 v.9 no.6 pp. 3456-3465
ISSN:
2042-650X
Subject:
Lepidium meyenii; active ingredients; adenosine; aerial parts; amino acids; animal models; ascorbic acid; benzyl isothiocyanate; blood serum; calcium; developmental stages; gastric emptying; gastrins; glucosinolates; intestines; mice; minerals; motilin; niacin; potassium; powders; saponins; vegetables; Andes region
Abstract:
... Lepidium meyenii Walp. (maca) has been utilized in the Andean region because of its edibleness and medicinal value. The aerial parts of maca (APM) were analyzed for protein, total sugar, vitamins, amino acids, and minerals and its characteristic active ingredients at five different growth stages. The results showed the high protein, total sugar, vitamin C, niacin, potassium, and calcium contents o ...
DOI:
10.1039/c8fo00405f

52. Anthelmintic activity of cytochrome P450 inhibitors miconazole and clotrimazole: in-vitro effect on the liver fluke Opisthorchis felineus

Author:
Viatcheslav A. Mordvinov; Alexander G. Shilov; Maria Y. Pakharukova
Source:
International journal of antimicrobial agents 2017 v.50 no.1 pp. 97-100
ISSN:
0924-8579
Subject:
Opisthorchis; adults; benzyl isothiocyanate; clotrimazole; cytochrome P-450; disulfiram; drugs; enzymes; genes; heme; inhibitory concentration 50; juveniles; ketoconazole; liver flukes; metacercariae; metyrapone; miconazole; mortality; opisthorchiasis; praziquantel; schistosomiasis; therapeutics; triadimenol
Abstract:
... Discovery of drugs for the treatment of opisthorchiasis and schistosomiasis is a high priority. The basic metabolic cytochrome P450 (CYP) system in parasitic flatworms contains a single gene. CYP of the liver fluke Opisthorchis felineus, the causative agent of opisthorchiasis, is important for survival of the worm, so it may be a promising target for therapeutics against liver fluke infection.The ...
DOI:
10.1016/j.ijantimicag.2017.01.037

53. Benzyl isothiocyanate ameliorates lipid accumulation in 3T3-L1 preadipocytes during adipocyte differentiation

Author:
Ying Liang; Ikumi Sasaki; Yuki Takeda; Beiwei Zhu; Shintaro Munemasa; Toshiyuki Nakamura; Yoshiyuki Murata; Yoshimasa Nakamura
Source:
Bioscience, biotechnology, and biochemistry 2018 v.82 no.12 pp. 2130-2139
ISSN:
1347-6947
Subject:
2-deoxyglucose; AMP-activated protein kinase; Brassicaceae; adipocytes; benzyl isothiocyanate; biotechnology; calves; fetal bovine serum; gene expression; glucose; glucose transporters; lactate dehydrogenase; lipids; mitogen-activated protein kinase; phosphorylation
Abstract:
... Benzyl isothiocyanate (BITC) is an organosulfur compound derived from cruciferous vegetables and papaya seeds. In this study, we investigated the effect of BITC on the lipid accumulation in 3T3-L1 preadipocytes during adipocyte differentiation. The treatment of BITC during the differentiation-inducing stage significantly ameliorated the lipid accumulation, whereas it had no inhibitory effect durin ...
DOI:
10.1080/09168451.2018.1514247

54. Benzyl isothiocyanate inhibits human brain glioblastoma multiforme GBM 8401 cell xenograft tumor in nude mice in vivo

Author:
Yi‐Shih Ma; Jen‐Jyh Lin; Chin‐Chung Lin; Jin‐Cherng Lien; Shu‐Fen Peng; Ming‐Jen Fan; Fei‐Ting Hsu; Jing‐Gung Chung
Source:
Environmental toxicology 2018 v.33 no.11 pp. 1097-1104
ISSN:
1520-4081
Subject:
animal models; benzyl isothiocyanate; body weight; brain; caspase-3; cell death; dimethyl sulfoxide; glioblastoma; humans; image analysis; immunohistochemistry; luciferase; males; mice; neoplasm cells; oral administration; photons
Abstract:
... Benzyl isothiocyanate (BITC), a member of isothiocyanates (ITCs), has been shown to induce cell death in many human cancer cells, but there is no further report to show BITC suppresses glioblastoma multiforme cells in vivo. In the present study, we investigate the effects of BITC on the inhibition of GBM 8401/luc2 cell generated tumor on athymic nude mice. We established a luciferase expressing st ...
DOI:
10.1002/tox.22581

55. Determination of isothiocyanate-protein conjugates in milk and curd after adding garden cress (Lepidium sativum L.)

Author:
Carla Kühn; Tobias von Oesen; Franziska S. Hanschen; Sascha Rohn
Source:
Food research international 2018 v.108 pp. 621-627
ISSN:
0963-9969
Subject:
Lepidium sativum; Lewis bases; allyl isothiocyanate; amino acid derivatives; benzyl isothiocyanate; bioavailability; cysteine; food matrix; health promotion; liquid chromatography; lysine; milk; milk proteins; neoplasms; pH; tandem mass spectrometry
Abstract:
... Isothiocyanates (ITC) play an important role in health promotion and cancer prevention due to their anti-bacterial, anti-inflammatory, and anti-cancerogenic properties. However, ITC are highly reactive so that a reaction with further food components is very likely. For example, a reaction of ITC with nucleophilic amino acid side chains of proteins such as cysteine and lysine can occur, reducing th ...
DOI:
10.1016/j.foodres.2018.04.001

56. Diverse Excretion Pathways of Benzyl Glucosinolate in Humans after Consumption of Nasturtium (Tropaeolum majus L.)—A Pilot Study

Author:
Carla Kühn; Franziska Kupke; Susanne Baldermann; Rebecca Klopsch; Evelyn Lamy; Silke Hornemann; Andreas F. H. Pfeiffer; Monika Schreiner; Franziska S. Hanschen; Sascha Rohn
Source:
Molecular nutrition & food research 2018 v.62 no.20 pp. e1800588
ISSN:
1613-4125
Subject:
Nasturtium; Tropaeolum majus; benzyl isothiocyanate; blood; breads; breathing; cyanides; excretion; freeze drying; glucosinolates; metabolites; urine
Abstract:
... SCOPE: Different metabolic and excretion pathways of the benzyl glucosinolate breakdown products benzyl isothiocyanate and benzyl cyanide are investigated to obtain information about their multiple fate after ingestion. Detailed focus is on the so far underestimated transformation/excretion pathways—protein conjugation and exhalation. METHODS AND RESULTS: Metabolites, protein conjugates, and non‐c ...
DOI:
10.1002/mnfr.201800588

57. Inhibition of phosphatidylinositide 3-kinase impairs the benzyl isothiocyanate-induced accumulation of autophagic molecules and Nrf2 in human colon cancer cells

Author:
Xiaoyang Liu; Naomi Abe-Kanoh; Yujia Liu; Beiwei Zhu; Shintaro Munemasa; Toshiyuki Nakamura; Yoshiyuki Murata; Yoshimasa Nakamura
Source:
Bioscience, biotechnology, and biochemistry 2017 v.81 no.11 pp. 2212-2215
ISSN:
1347-6947
Subject:
benzyl isothiocyanate; biotechnology; colorectal neoplasms; humans; phosphatidylinositol 3-kinase
Abstract:
... The regulating role of phosphatidylinositide 3-kinase (PI3K) in benzyl isothiocyanate (BITC)-induced Nrf2 activation, contributing to the inducible expression of cytoprotective genes, was investigated. BITC significantly enhanced the accumulation of Nrf2 as well as autophagic molecules in human colorectal cancer HCT-116 cells. Experiments using a PI3K-specific inhibitor suggested that PI3K plays t ...
DOI:
10.1080/09168451.2017.1374830

58. Mechanistic evaluation of phytochemicals in breast cancer remedy: current understanding and future perspectives

Author:
Muhammad Younas; Christophe Hano; Nathalie Giglioli-Guivarc'h; Bilal Haider Abbasi
Source:
RSC advances 2018 v.8 no.52 pp. 29714-29744
ISSN:
2046-2069
Subject:
Vinca; adjuvants; adverse effects; alkaloids; angiogenesis; apigenin; apoptosis; benzyl isothiocyanate; breast neoplasms; cell proliferation; chemoprevention; cost effectiveness; curcumin; death; drug resistance; drugs; epigallocatechin gallate; genes; genistein; ginsenosides; hormone replacement therapy; kaempferol; luteolin; metastasis; naringenin; neoplasm cells; pterostilbene; quercetin; quinones; radiotherapy; resveratrol; signal transduction; stem cells; sulforaphane; women
Abstract:
... Breast cancer is one of the most commonly diagnosed cancers around the globe and accounts for a large proportion of fatalities in women. Despite the advancement in therapeutic and diagnostic procedures, breast cancer still represents a major challenge. Current anti-breast cancer approaches include surgical removal, radiotherapy, hormonal therapy and the use of various chemotherapeutic drugs. Howev ...
DOI:
10.1039/c8ra04879g

59. Raman spectroscopy, electronic microscopy and SPME-GC-MS to elucidate the mode of action of a new antimicrobial food packaging material

Author:
Isabel Clemente; Margarita Aznar; Jesús Salafranca; Cristina Nerín
Source:
Analytical and bioanalytical chemistry 2017 v.409 no.4 pp. 1037-1048
ISSN:
1618-2642
Subject:
Aspergillus ochraceus; Escherichia coli; Penicillium verrucosum; Raman spectroscopy; active food packaging; allyl isothiocyanate; anti-infective agents; antimicrobial packaging; antimicrobial properties; bacteria; benzyl isothiocyanate; cinnamon; essential oils; gas chromatography-mass spectrometry; mechanism of action; molds (fungi); ochratoxin A; oregano; packaging materials; scanning electron microscopy; solid phase microextraction
Abstract:
... One critical challenge when developing a new antimicrobial packaging material is to demonstrate the mode of action of the antimicrobials incorporated into the packaging. For this task, several analytical techniques as well as microbiology are required. In this work, the antimicrobial properties of benzyl isothiocyanate, allyl isothiocyanate and essential oils of cinnamon and oregano against severa ...
DOI:
10.1007/s00216-016-0022-y

60. Use of potential dietary phytochemicals to target miRNA: Promising option for breast cancer prevention and treatment?

Author:
Md. Abu Sayeed; Massimo Bracci; Raffaella Lazzarini; Marco Tomasetti; Monica Amati; Guendalina Lucarini; Roberto Di Primio; Lory Santarelli
Source:
Journal of functional foods 2017 v.28 pp. 177-193
ISSN:
1756-4646
Subject:
adverse effects; benzyl isothiocyanate; breast neoplasms; capsaicin; curcumin; diallyl disulfide; drug resistance; ellagic acid; epigallocatechin; females; genistein; metastasis; microRNA; oleanolic acid; phytopharmaceuticals; quercetin; resveratrol; therapeutics; ursolic acid
Abstract:
... Breast cancer is one of the most common forms of cancers in females. Traditional therapies are associated with adverse side effects, and drug resistance, recurrence, and lack of treatment in metastasis are the major problems. Accumulating evidence indicates that dietary phytochemicals may exert therapeutic effects by regulating miRNA expression. A number of dietary phytochemicals have been tested ...
DOI:
10.1016/j.jff.2016.11.008

61. Benzyl isothiocyanate ameliorates acetaldehyde-induced cytotoxicity by enhancing aldehyde dehydrogenase activity in murine hepatoma Hepa1c1c7 cells

Author:
Yujia Liu; Momoko Yamanaka; Naomi Abe-Kanoh; Xiaoyang Liu; Beiwei Zhu; Shintaro Munemasa; Toshiyuki Nakamura; Yoshiyuki Murata; Yoshimasa Nakamura
Source:
Food and chemical toxicology 2017 v.108 pp. 305-313
ISSN:
0278-6915
Subject:
NAD(P)H dehydrogenase (quinone); Western blotting; aldehyde dehydrogenase; benzyl isothiocyanate; cytotoxicity; enzyme activity; genes; heme oxygenase (biliverdin-producing); hepatoma; mice; mitochondria; protein synthesis; toxicology; vegetables
Abstract:
... In the present study, we assessed benzyl isothiocyanate (BITC), an organosulfur compound from cruciferous vegetables, as a potential inducer of aldehyde dehydrogenase (ALDH) activity using murine hepatoma Hepa1c1c7 cells. BITC was shown to enhance not only the total ALDH activity, but also the ALDH activity of the cytosolic/microsomal and mitochondrial fraction. BITC also significantly increased t ...
DOI:
10.1016/j.fct.2017.08.016

62. Chemical composition and antifungal activity of Carica papaya Linn. seed essential oil against Candida spp.

Author:
X. He; Y. Ma; G. Yi; J. Wu; L. Zhou; H. Guo
Source:
Letters in applied microbiology 2017 v.64 no.5 pp. 350-354
ISSN:
0266-8254
Subject:
Candida parapsilosis; Carica papaya; antifungal agents; antifungal properties; benzyl isothiocyanate; chemical composition; diameter; drug resistance; essential oils; gas chromatography-mass spectrometry; minimum inhibitory concentration; oils; seeds; yeasts
Abstract:
... In recent years, the incidence of clinical yeast infections has increased dramatically. Due to the extensive use of broad‐spectrum antifungal agents, there has been a notable increase in drug resistance among infections yeast species. As one of the most popular natural antimicrobial agents, essential oils (EOs) have attracted a lot of attention from the scientific community. The aim of this study ...
DOI:
10.1111/lam.12711

63. Cytotoxicity effect of water extracts of Moringa oleifera leaves and seeds against MCF-7 cells

Author:
M. H. A. Rahaman; N. H. A. Kadir; N. M. Amin; W. B. W. Omar
Source:
Acta horticulturae 2017 v. no.1158 pp. 279-286
ISSN:
0567-7572
Subject:
Brassicaceae; Caricaceae; Moringa oleifera; Western blotting; acridine orange; adenocarcinoma; antineoplastic activity; benzyl isothiocyanate; breast neoplasms; breasts; cell death; cytotoxicity; glucosinolates; inhibitory concentration 50; leaves; mitogen-activated protein kinase; plant tissues; propidium; protein synthesis; seeds; staining; thioglucosidase; viability
Abstract:
... Glucosinolates are the most abundant compounds in Brassicaceae, Moringaceae and Caricaceae. The glucosinolates will be hydrolysed to their degradation products by myrosinase when plant tissues are disrupted. Water extracts of Moringa oleifera leaves (MOL) and seeds (MOS) were screened for cytotoxic activity against breast adenocarcinoma (MCF-7) cells. Benzyl isothiocyanate (BITC) which possesses a ...
DOI:
10.17660/ActaHortic.2017.1158.31

64. Effect of an active label based on benzyl isothiocyanate on the morphology and ochratoxins production of Aspergillus ochraceus

Author:
Isabel Clemente; Margarita Aznar; Cristina Nerín
Source:
Food research international 2017 v.101 pp. 61-72
ISSN:
0963-9969
Subject:
Aspergillus ochraceus; active food packaging; analytical methods; benzyl isothiocyanate; cleistothecia; mechanism of action; metabolism; ochratoxin A; ochratoxin B; protocols; scanning electron microscopy; sclerotia; secondary metabolites
Abstract:
... The aim of this work was the study of the main effects of benzyl isothiocyanate (BITC) on A. ochraceus morphology and on its production metabolism of ochratoxins. This compound was evaluated as active agent of an antimicrobial label in food packaging. Microbiological studies showed a slowdown in mould growth when the active material was applied to A. ochraceus and the presence of three different a ...
DOI:
10.1016/j.foodres.2017.08.060

65. Effects of natural antimicrobials on prevention and reduction of bacterial cross-contamination during the washing of ready-to-eat fresh-cut lettuce

Author:
Cristina Pablos; Alba Fernández; Alison Thackeray; Javier Marugán
Source:
Food science and technology international v.23 no.5 pp. 403-414
ISSN:
1082-0132
Subject:
Salmonella; anti-infective agents; antimicrobial properties; bacteria; benzyl isothiocyanate; chitosan; chlorine; cross contamination; fresh-cut produce; lettuce; microbiological quality; minimum inhibitory concentration; plate count; ready-to-eat foods; washing
Abstract:
... Microbiological safety of the fresh-cut produce may not be guaranteed if the quality of wash water is not maintained. The use of natural antimicrobials as alternative to chlorine may offer interesting possibilities for disinfecting wash water. Antimicrobial properties of allyl- and benzyl-isothiocyanates, respectively, and chitosan against Salmonella spp. were evaluated by standard plate count. Mi ...
DOI:
10.1177/1082013217697851

66. Inhibition of phosphatidylinositide 3-kinase ameliorates antiproliferation by benzyl isothiocyanate in human colon cancer cells

Author:
Xiaoyang Liu; Chiaki Takano; Tomomi Shimizu; Shintaro Yokobe; Naomi Abe-Kanoh; Beiwei Zhu; Toshiyuki Nakamura; Shintaro Munemasa; Yoshiyuki Murata; Yoshimasa Nakamura
Source:
Biochemical and biophysical research communications 2017 pp. -
ISSN:
0006-291X
Subject:
apoptosis; benzyl isothiocyanate; colorectal neoplasms; enzyme activity; humans; neoplasm cells; phosphatidylinositol 3-kinase; protein-tyrosine-phosphatase
Abstract:
... In the present study, we clarified the role of phosphatidylinositide 3-kinase (PI3K) in antiproliferation induced by benzyl isothiocyanate (BITC) in human colorectal cancer cells. BITC simultaneously activated the PI3K/Akt/forkhead box O (FoxO) pathway, whereas it significantly inhibited the proliferation in human colorectal cancer cells. Inhibitory experiments using a PI3K selective inhibitor, LY ...
DOI:
10.1016/j.bbrc.2017.07.078

67. Oral administration of nasturtium affects peptide YY secretion in male subjects

Author:
Sonja Schiess; Stefanie Platz; Margrit Kemper; Monika Schreiner; Inga Mewis; Sascha Rohn; Christiane Bumke‐Vogt; Olga Pivovarova; Andreas F.H. Pfeiffer
Source:
Molecular nutrition & food research 2017 v.61 no.8 pp. -
ISSN:
1613-4125
Subject:
Nasturtium; anti-inflammatory activity; benzyl isothiocyanate; blood sampling; blood serum; c-peptide; chemoprevention; digestive system; energy; freeze drying; glucagon-like peptide 1; glucosinolates; humans; leaves; males; neoplasms; obesity; oral administration; peptide YY; rodents; secretion; special diets
Abstract:
... SCOPE: Nasturtium plants contain the glucosinolate glucotropaeolin and its corresponding breakdown product benzyl isothiocyanate (BITC), the latter being intensively studied with regard to cancer chemoprevention and anti‐inflammatory properties. In addition, recent research has shown that isothiocyanates are able to activate the release of several gut hormones in vitro and in rodent studies. Here, ...
DOI:
10.1002/mnfr.201600886

68. Benzyl isothiocyanate (BITC) induces apoptosis of GBM 8401 human brain glioblastoma multiforms cells via activation of caspase‐8/Bid and the reactive oxygen species‐dependent mitochondrial pathway

Author:
Hung‐Sheng Shang; Yung‐Luen Shih; Tai‐Jung Lu; Ching‐Hsiao Lee; Shu‐Ching Hsueh; Yu‐Cheng Chou; Hsu‐Feng Lu; Nien‐Chieh Liao; Jing‐Gung Chung
Source:
Environmental toxicology 2016 v.31 no.12 pp. 1751-1760
ISSN:
1520-4081
Subject:
Western blotting; apoptosis; benzyl isothiocyanate; brain; calcium; calpain; caspase-3; caspase-8; cytochrome c; dose response; flow cytometry; humans; membrane potential; mitochondria; mitochondrial membrane; neoplasm cells; neoplasms; pro-apoptotic proteins; signal transduction
Abstract:
... Benzyl isothiocyanate (BITC) is one of member of the isothiocyanate family which has been shown to induce cancer cell apoptosis in many human cancer cells. In the present study, we investigated the effects of BITC on the growth of GBM 8401 human brain glioblastoma multiforms cells. Results indicated that BITC‐induced cell morphological changes decreased in the percentage of viable GBM8401 cells an ...
DOI:
10.1002/tox.22177

69. Benzyl isothiocyanate but not benzyl nitrile from Brassicales plants dually blocks the COX and LOX pathway in primary human immune cells

Author:
Corinna Herz; Melinda-Rita Márton; Hoai Thi Thu Tran; Carsten Gründemann; Jana Schell; Evelyn Lamy
Source:
Journal of functional foods 2016 v.23 pp. 135-143
ISSN:
1756-4646
Subject:
Brassicales; T-lymphocytes; anti-inflammatory activity; benzyl isothiocyanate; glucosinolates; humans; inflammation; lipopolysaccharides; nitriles; prostaglandins
Abstract:
... The main products formed from glucosinolates from Brassicales plants are isothiocyanates (ITCs) and nitriles. ITCs are known for their diverse bioactive potential, knowledge about the bioactivity of food-borne nitriles is poor, though. Anti-inflammation by benzyl ITC (BITC) and its nitrile has not so far been studied in human immune cells. Here, the potential of BITC to inhibit the lipopolysacchar ...
DOI:
10.1016/j.jff.2016.02.034

70. Benzyl isothiocyanate promotes apoptosis of oral cancer cells via an acute redox stress-mediated DNA damage response

Author:
Yao-Tsung Yeh; Yen-Nien Hsu; Sheng-Yun Huang; Jian-Sheng Lin; Zi-Feng Chen; Nan-Haw Chow; Shu-Hui Su; Huey-Wen Shyu; Ching-Chiang Lin; Wu-Tein Huang; Hua Yeh; Yu-chia chih; Yu-Hsuan Huang; Shu-Jem Su
Source:
Food and chemical toxicology 2016 v.97 pp. 336-345
ISSN:
0278-6915
Subject:
DNA damage; acetylcysteine; antineoplastic activity; apoptosis; benzyl isothiocyanate; beta-mercaptoethanol; catalase; cyclins; dithiothreitol; drugs; free radical scavengers; glutathione; humans; mannitol; membrane potential; mitochondrial membrane; mouth neoplasms; neoplasm cells; nitric oxide; reactive oxygen species; toxicity; toxicology
Abstract:
... Benzyl isothiocyanate (BITC) is a cruciferous vegetable-derived compound with anticancer properties in human cancer cells. However, its anticancer potential and underlying mechanisms remain absent in human oral cancer cells. Results indicate that BITC inhibits growth, promotes G2/M phase arrest and triggers apoptosis of OC2 cells with a minimal toxicity to normal cells. BITC-induced cell death was ...
DOI:
10.1016/j.fct.2016.09.028

71. Bioavailability and metabolism of benzyl glucosinolate in humans consuming Indian cress (Tropaeolum majus L.)

Author:
Stefanie Platz; Carla Kühn; Sonja Schiess; Monika Schreiner; Margrit Kemper; Olga Pivovarova; Andreas F. H. Pfeiffer; Sascha Rohn
Source:
Molecular nutrition & food research 2016 v.60 no.3 pp. 652-660
ISSN:
1613-4125
Subject:
Tropaeolum majus; benzyl isothiocyanate; bioavailability; blood; chemoprevention; cross-over studies; drugs; excretion; foods; freeze drying; glucosinolates; humans; ingestion; men; metabolism; metabolites; neoplasms; resorption; urine
Abstract:
... SCOPE: Benzyl isothiocyanate (BITC), which occurs in Brassicales, has demonstrated chemopreventive potency and cancer treatment properties in cell and animal studies. However, fate of BITC in human body is not comprehensively studied. Therefore, the present human intervention study investigates the metabolism of the glucosinolate (GSL) glucotropaeolin and its corresponding BITC metabolites. Analyz ...
DOI:
10.1002/mnfr.201500633

72. Effect of Essential Oils on Germination and Growth of Some Pathogenic and Spoilage Spore-Forming Bacteria

Author:
Voundi Stève Olugu; Nyegue Maximilienne; Lazar Iuliana; Raducanu Dumitra; Ndoye Florentine Foe; Stamate Marius; Etoa François-Xavier
Source:
Foodborne pathogens & disease 2015 v.12 no.6 pp. 551-559
ISSN:
1556-7125
Subject:
Drypetes; Geobacillus stearothermophilus; antibacterial properties; bacterial spores; benzyl isothiocyanate; essential oils; food industry; food preservatives; food safety; gas chromatography; mass spectrometry; minimum inhibitory concentration; oils; spoilage; spore germination; spore-forming bacteria; synergism; vegetative cells; vegetative growth
Abstract:
... The use of essential oils as a food preservative has increased due to their capacity to inhibit vegetative growth of some bacteria. However, only limited data are available on their effect on bacterial spores. The aim of the present study was to evaluate the effect of some essential oils on the growth and germination of three Bacillus species and Geobacillus stearothermophilus. Essential oils were ...
DOI:
10.1089/fpd.2014.1892

73. Glutathione and its related enzymes in the gonad of Nile Tilapia (Oreochromis niloticus)

Author:
R. R. Hamed; N. S. M. Saleh; A. Shokeer; R. A. Guneidy; S. S. Abdel-Ghany
Source:
Fish physiology and biochemistry 2016 v.42 no.1 pp. 353-364
ISSN:
0920-1742
Subject:
Oreochromis niloticus; allyl isothiocyanate; animal ovaries; antioxidants; benzyl isothiocyanate; catalase; eggs; enzyme kinetics; excretion; glutathione; glutathione peroxidase; glutathione transferase; glutathione-disulfide reductase; inhibitory concentration 50; molecular weight; pH; polyacrylamide gel electrophoresis; protein content; testes
Abstract:
... Glutathione (GSH) concentration, the activity of its metabolizing enzymes, glutathione transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR), and the antioxidant enzyme catalase (CAT) in O. niloticus ovary and testis were examined. GSH concentration of O. niloticus testis exhibited high concentration (129 ± 21 nmol/g tissue) compared with GSH concentration (49.2 ± 8.3 nmol/g ...
DOI:
10.1007/s10695-015-0143-9

74. Phytochemical investigation and in vitro antibacterial activity of essential oils from Cleome droserifolia (Forssk.) Delile and C. trinervia Fresen. (Cleomaceae)

Author:
Riyadh Muhaidat; Mahmoud A. Al-Qudah; Omar Samir; Jacob H. Jacob; Emad Hussein; Ibrahim N. Al-Tarawneh; Emad Bsoul; Sultan T. Abu Orabi
Source:
South African journal of botany 2015 v.99 pp. 21-28
ISSN:
0254-6299
Subject:
Cleome; Gram-negative bacteria; antibacterial properties; benzyl isothiocyanate; drug resistance; essential oils; evolution; gas chromatography-mass spectrometry; in vitro studies; minimum inhibitory concentration; monoterpenoids; nitrogen; oils; pathogens; petioles; scanning electron microscopy; sesquiterpenoids; trichomes; Jordan
Abstract:
... The evolution of drug resistant strains of microbial pathogens provides an urgent need to develop novel therapeutical regimes with resistance-combating capacity. In this study, the phytochemical composition and antibacterial activities of essential oils from Cleome droserifolia and Cleome trinervia growing in Jordan desert were investigated. Hydro-distilled oils were analyzed using GC–MS and evalu ...
DOI:
10.1016/j.sajb.2015.03.184

75. Raman Imaging Spectroscopy as a Tool To Investigate the Cell Damage on Aspergillus ochraceus Caused by an Antimicrobial Packaging Containing Benzyl Isothiocyanate

Author:
Clemente Isabel; Aznar Margarita; Nerín Cristina
Source:
Analytical chemistry 2016 v.88 no.9 pp. 4772-4779
ISSN:
1520-6882
Subject:
Aspergillus ochraceus; amino acids; antimicrobial packaging; benzyl isothiocyanate; carbohydrates; cell cycle; chemometrics; direct contact; food packaging; genes; hyphae; image analysis; lipids; metabolism; ochratoxin A; packaging materials; principal component analysis; proteins; spectroscopy; spores; vapors
Abstract:
... Raman imaging spectroscopy is a nondestructive analytical method that can be a useful tool to obtain detailed information about the molecular composition and morphology of biological samples. Its high spatial resolution was used to collect spectra of Aspergillus ochraceus, a mold producer of ochratoxin A (OTA), in order to investigate the cell damage caused on it by the action of the antimicrobial ...
DOI:
10.1021/acs.analchem.6b00116

76. Sensory analysis and head-space aroma volatiles for the characterization of capers from different geographic origin

Author:
Concetta Condurso; Agata Mazzaglia; Gianluca Tripodi; Fabrizio Cincotta; Giovanna Dima; Carmela Maria Lanza; Antonella Verzera
Source:
Journal of essential oil research 2016 v.28 no.3 pp. 185-192
ISSN:
2163-8152
Subject:
Capparis spinosa; alcohols; aldehydes; benzyl isothiocyanate; buds; essential oils; gas chromatography-mass spectrometry; headspace analysis; islands; methyl isothiocyanate; multivariate analysis; odor compounds; odors; provenance; sensory evaluation; solid phase microextraction; sulfur; Italy
Abstract:
... Characterization of capers (Capparis spinosa L. Capparaceae) was achieved by measurement of aroma compounds and sensory descriptors. Different capper samples (the floral buds of the plants) coming from Pantelleria and Salina Islands (Italy) were analyzed. Head-space aroma volatiles were extracted and analyzed by SPME-GC-MS. In all, more than one-hundred volatile compounds were identified and the q ...
DOI:
10.1080/10412905.2015.1113205

77. A novel impact of a novel weapon: allelochemicals in Alliaria petiolata disrupt the legume-rhizobia mutualism

Author:
Cristina Portales-Reyes; Tina Van Doornik; Elizabeth H. Schultheis; Tomomi Suwa
Source:
Biological invasions 2015 v.17 no.9 pp. 2779-2791
ISSN:
1387-3547
Subject:
Alliaria petiolata; allelochemicals; allyl isothiocyanate; benzyl isothiocyanate; ecological invasion; greenhouse experimentation; greenhouses; introduced species; invasive species; leaf extracts; mutualism; nodulation; phytotoxicity; plant growth; soil; soil microorganisms
Abstract:
... Some introduced species become invasive by releasing novel allelochemicals into the soil, directly harming nearby plants and soil microbes. Alliaria petiolata (garlic mustard) is an invasive plant, well known to excrete a suite of phytotoxic and anti-microbial allelochemicals, including allyl isothiocyanate (AITC) and benzyl isothiocyanate (BITC). While the effects of these chemicals on plant-myco ...
DOI:
10.1007/s10530-015-0913-4

78. Benzyl isothiocyanate inhibits IL-13 expression in human basophilic KU812 cells

Author:
Yue Tang; Naomi Abe; Motoko Yoshimoto; Beiwei Zhu; Yoshiyuki Murata; Yoshimasa Nakamura
Source:
Bioscience, biotechnology, and biochemistry 2015 v.79 no.1 pp. 159-163
ISSN:
1347-6947
Subject:
Brassicaceae; benzyl isothiocyanate; biotechnology; calcium; humans; inflammation; interleukin-13; mitogen-activated protein kinase; transcription (genetics)
Abstract:
... An allergen-stimulating cytokine, interleukin-13 (IL-13), plays a significant role in allergic inflammation. Benzyl isothiocyanate (BITC), derived from several cruciferous vegetables, significantly suppressed the IL-13 expression in the calcium ionophore-stimulated human basophilic KU812 cells. Down-regulation of phosphorylated mitogen-activated protein kinases as well as nuclear transcriptional f ...
DOI:
10.1080/09168451.2014.963503

79. Benzyl isothiocyanate: maximising production in papaya tissue extracts

Author:
D. J. Williams; S. Pun; N. Alotaibi; T. J. O'Hare; Y. Sultanbawa
Source:
Acta horticulturae 2015 no.1106 pp. 101-108
ISSN:
0567-7572
Subject:
Brassica; Carica papaya; Lepidium sativum; benzyl isothiocyanate; cultivars; cutting; ethanol; glucosinolates; hydrolysis; mastication; proteins; seed mixtures; seeds; solvents; thioglucosidase; tissues; vegetables
Abstract:
... Although mainly grown for its sweet flavoured fruit, papaya (Carica papaya) has also been used for pharmacological purposes for many years. The reasons for use are varied with one of the best known being its anti-fungal action. Benzyl isothiocyanate (BITC) is the constituent most often implicated in this activity. Isothiocyanates are formed when the enzyme myrosinase catalyses the hydrolysis of th ...
DOI:
10.17660/ActaHortic.2015.1106.16

80. Cytotoxic effect of Reseda lutea L.: A case of forgotten remedy

Author:
Niko S. Radulović; Dragan B. Zlatković; Tatjana Ilić-Tomić; Lidija Senerović; Jasmina Nikodinovic-Runic
Source:
Journal of ethnopharmacology 2014 v.153 no.1 pp. 125-132
ISSN:
0378-8741
Subject:
Reseda lutea; apoptosis; benzyl isothiocyanate; cell cycle; cell lines; cytotoxicity; fibroblasts; flowers; fruits; gas chromatography-mass spectrometry; melanoma; metabolites; nuclear magnetic resonance spectroscopy; traditional medicine
Abstract:
... Reseda lutea L. (Resedaceae) or Wild Mignonette is a widely distributed plant species. Pliny the Elder (AD 23–AD 79), a Roman scholar and naturalist, reported the use of R. lutea for reducing tumors in his Historia naturalis. Accounts of the beneficial effects of R. lutea in tumor treatment could also be found in the works of later authors, such as Étienne François Geoffroy (1672–1731) and Samuel ...
DOI:
10.1016/j.jep.2014.01.034

81. Derivatization of isothiocyanates and their reactive adducts for chromatographic analysis

Author:
Niels Agerbirk; Gina Rosalinda De Nicola; Carl Erik Olsen; Caroline Müller; Renato Iori
Source:
Phytochemistry 2015 v.118 pp. 109-115
ISSN:
0031-9422
Subject:
ammonia; benzyl isothiocyanate; biochemical compounds; cabbage; derivatization; glycosylation; hydrogen; hydrophilicity; hydrophobicity; insects; leaves; liquid chromatography; mass spectrometry; sodium; thiourea
Abstract:
... Isothiocyanates form adducts with a multitude of biomolecules, and these adducts need analytical methods. Likewise, analytical methods for hydrophilic isothiocyanates are needed. We considered reaction with ammonia to form thiourea derivatives. The hydrophilic, glycosylated isothiocyanate moringin, 4-(α-l-rhamnopyranosyloxy)benzyl isothiocyanate, was efficiently derivatized to the thiourea derivat ...
DOI:
10.1016/j.phytochem.2015.06.004

82. Glycyl endopeptidase from papaya latex: Partial purification and use for production of fish gelatin hydrolysate

Author:
Supatra Karnjanapratum; Soottawat Benjakul
Source:
Food chemistry 2014 v.165 pp. 403-411
ISSN:
0308-8146
Subject:
ammonium sulfate; benzyl isothiocyanate; cultivars; fish; free radical scavengers; gelatin; hydrolysates; latex; magnesium sulfate; odors; polyethylene glycol
Abstract:
... An aqueous two-phase system (ATPS) in combination with ammonium sulphate ((NH4)2SO4) precipitation was applied to fractionate glycyl endopeptidase from the papaya latex of Red Lady and Khack Dum cultivars. ATPS containing polyethylene glycol (PEG 2000 and 6000) and salts ((NH4)2SO4 and MgSO4) at different concentrations were used. Glycyl endopeptidase with high purification fold (PF) and yield was ...
DOI:
10.1016/j.foodchem.2014.05.123

83. Inhibition of the general soil microbial community by allyl-isothiocyanate and benzyl-isothiocyanate

Author:
Beck Alyssa M.; Marsh Tamara L.
Source:
Bios 2015 v.86 no.1 pp. 31-37
ISSN:
0005-3155
Subject:
Alliaria petiolata; allyl isothiocyanate; benzyl isothiocyanate; chemical concentration; chemical treatment; forest ecosystems; microbial growth; minimum inhibitory concentration; soil microorganisms; understory; United States
Abstract:
... Invasion of Alliaria petiolata (garlic mustard) has become increasingly problematic, as it has disrupted the native ecosystem of the forest understory throughout most of the United States. A key element of garlic mustard invasion is the production of two compounds that have been shown to be inhibitory at relatively low concentrations, allyl-isothiocyanate (AITC) and benzyl-isothiocyanate (BITC). T ...
DOI:
10.1893/0005-3155-86.1.31

84. Odour-active compounds in papaya fruit cv. Red Maradol

Author:
Jorge A. Pino
Source:
Food chemistry 2014 v.146 pp. 120-126
ISSN:
0308-8146
Subject:
benzyl isothiocyanate; microextraction; odor compounds; odors
Abstract:
... Application of solid-phase microextraction and simultaneous distillation–extraction combined with GC–FID, GC–MS, aroma extract dilution analysis, and odour activity value were used to analyse volatile compounds from papaya fruit cv. Red Maradol and to estimate the most odour-active compounds. The analyses led to the identification of 137 compounds; 118 of them were positively identified. Twenty-fi ...
DOI:
10.1016/j.foodchem.2013.09.031
PubMed:
24176322

85. Pharmacological synergism of bee venom and melittin with antibiotics and plant secondary metabolites against multi-drug resistant microbial pathogens

Author:
Issam AL-Ani; Stefan Zimmermann; Jürgen Reichling; Michael Wink
Source:
Phytomedicine 2015 v.22 pp. 245-255
ISSN:
0944-7113
Subject:
Apoidea; DNA; Escherichia coli; Gram-negative bacteria; additive effect; amikacin; antibacterial properties; antibiotic resistance; antifungal properties; benzyl isothiocyanate; berberine; carvacrol; drugs; melittin; methicillin-resistant Staphylococcus aureus; minimum inhibitory concentration; multiple drug resistance; oxacillin; pathogens; sanguinarine; secondary metabolites; synergism; vancomycin; venoms
Abstract:
... The goal of this study was to investigate the antimicrobial activity of bee venom and its main component, melittin, alone or in two-drug and three-drug combinations with antibiotics (vancomycin, oxacillin, and amikacin) or antimicrobial plant secondary metabolites (carvacrol, benzyl isothiocyanate, the alkaloids sanguinarine and berberine) against drug-sensitive and antibiotic-resistant microbial ...
DOI:
10.1016/j.phymed.2014.11.019

86. Preparation and characterization of inclusion complex of benzyl isothiocyanate extracted from papaya seed with β-cyclodextrin

Author:
Wenzhao Li; Xiaoyu Liu; Qingfeng Yang; Ning Zhang; Yideng Du; Huaping Zhu
Source:
Food chemistry 2015 v.184 pp. 99-104
ISSN:
0308-8146
Subject:
Fourier transform infrared spectroscopy; X-radiation; antibiotics; benzyl isothiocyanate; beta-cyclodextrin; nuclear magnetic resonance spectroscopy; particle size distribution; solubility; thermal analysis; thermodynamics
Abstract:
... The inclusion complex of benzyl isothiocyanate (BITC), extracted from papaya seed with β-cyclodextrin (β-CD), was prepared. Different analytical techniques, such as Fourier transform infrared spectroscopy, thermal analysis, X-ray diffractometry, particle size distribution analysis and 1H Nuclear magnetic resonance analysis, were used to investigate the characterization of the inclusion complex (BI ...
DOI:
10.1016/j.foodchem.2015.03.091

87. A chemical approach for the reduction of beauvericin in a solution model and in food systems

Author:
F.B. Luciano; G. Meca; L. Manyes; J. Mañes
Source:
Food and chemical toxicology 2014 v.64 pp. 270-274
ISSN:
0278-6915
Subject:
Fusarium; barley; benzyl isothiocyanate; bioactive compounds; corn; crops; dose response; flour; fumigants; liquid chromatography; metabolism; mycotoxins; pH; phosphates; rice; seeds; toxicology; wheat
Abstract:
... Beauvericin (BEA) is a bioactive compound produced by the secondary metabolism of several Fusarium strains with a strong antibacterial, antifungal, and insecticidal activities.This study evaluated the reduction of BEA added at 25mg/kg in phosphate buffer saline (PBS) solutions at pH of 4, 7 and 10, or to different cereal products (kernels and flours) by the bioactive compounds phenyl isothiocyanat ...
DOI:
10.1016/j.fct.2013.11.021

88. Comparison of antioxidant, anti-inflammatory, antimicrobial activity and chemical composition of aqueous and hydroethanolic extracts of the herb of Tropaeolum majus L.

Author:
Agnieszka Bazylko; Sebastian Granica; Agnieszka Filipek; Jakub Piwowarski; Joanna Stefańska; Ewa Osińska; Anna K. Kiss
Source:
Industrial crops and products 2013 v.50 pp. 88-94
ISSN:
0926-6690
Subject:
Nasturtium; Tropaeolum majus; antimicrobial properties; antioxidant activity; benzyl isothiocyanate; chemical composition; cinnamic acid; flavonoids; freeze drying; humans; hyaluronoglucosaminidase; hydrogen peroxide; neutrophils; nitric oxide; nitrogen; quinic acid; temperature
Abstract:
... The aim of this study was comparison of antioxidant, anti-inflammatory, and antimicrobial activity, as well as chemical composition of extracts of Tropaeolum majus L. herb. Aqueous and hydroethanolic extracts derived from dried and freeze-dried nasturtium herb, prepared both at room temperature and at 90°C, were studied. In the studies there were no significant differences between antioxidant acti ...
DOI:
10.1016/j.indcrop.2013.07.003

89. Floral Scent Composition of Moringa oleifera Lam.

Author:
Jorge A. Pino
Source:
Journal of essential oil-bearing plants 2013 v.16 no.3 pp. 315-317
ISSN:
0976-5026
Subject:
sesquiterpenoids; volatile compounds; capillary gas chromatography; Moringa oleifera; monoterpenoids; flowers; benzyl isothiocyanate; nitrogen compounds; mass spectrometry; odors; alpha-terpineol
Abstract:
... The volatile compounds of the flowers of Moringa oleifera Lam. were analyzed by simultaneous distillation-extraction and capillary gas chromatography/mass spectrometry. Seventy-four compounds were identified, representing 99.8 % of the total composition. The volatile fraction was characterized by aliphatic compounds (34.2 %), nitrogen-compounds (16.6 %), oxygenated monoterpenes (16.0 %), monoterpe ...
DOI:
10.1080/0972060X.2013.813189

90. Benzyl Isothiocyanate (BITC) Induces G2/M Phase Arrest and Apoptosis in Human Melanoma A375.S2 Cells through Reactive Oxygen Species (ROS) and both Mitochondria-Dependent and Death Receptor-Mediated Multiple Signaling Pathways

Author:
Huang Su-Hua; Wu Liu-Wei; Huang An-Cheng; Yu Chien-Chih; Lien Jin-Cherng; Huang Yi-Ping; Yang Jai-Sing; Yang Jen-Hung; Hsiao Yu-Ping; Wood W. Gibson; Yu Chun-Shu; Chung Jing-Gung
Source:
Journal of agricultural and food chemistry 2012 v.60 no.2 pp. 665-675
ISSN:
1520-5118
Subject:
Western blotting; apoptosis; benzyl isothiocyanate; caspase-3; cell cycle; cell growth; flow cytometry; humans; melanoma; membrane potential; propidium; reactive oxygen species; signal transduction
Abstract:
... Benzyl isothiocyanates (BITC), a member of the isothiocyanate (ITC) family, inhibits cell growth and induces apoptosis in many types of human cancer cell lines. The present study investigated mechanisms underlying BITC-induced apoptosis in A375.S2 human melanoma cancer cells. To observe cell morphological changes and viability, flow cytometric assays, cell counting, and a contrast-phase microscopi ...
DOI:
10.1021/jf204193v
PubMed:
22148415

91. Determination of benzyl isothiocyanate metabolites in human plasma and urine by LC-ESI-MS/MS after ingestion of nasturtium (Tropaeolum majus L.)

Author:
Stefanie Platz; Carla Kühn; Sonja Schiess; Monika Schreiner; Inga Mewis; Margrit Kemper; Andreas Pfeiffer; Sascha Rohn
Source:
Analytical and bioanalytical chemistry 2013 v.405 no.23 pp. 7427-7436
ISSN:
1618-2642
Subject:
Nasturtium; Tropaeolum majus; benzyl isothiocyanate; humans; ingestion; liquid chromatography; metabolites; monitoring; solid phase extraction; tandem mass spectrometry; urine
Abstract:
... A liquid chromatography–tandem mass spectrometry (LC-MS/MS) method was developed and validated to determine the concentration of benzyl isothiocyanate (BITC) metabolites in human plasma and urine. In this study, the following BITC metabolites have been considered: BITC–glutathione, BITC–cysteinylglycine, BITC–cysteine, and BITC–N-acetyl-L-cysteine. The assay development included: (1) synthesis of ...
DOI:
10.1007/s00216-013-7176-7

92. Determination of sinigrin, sinalbin, allyl- and benzyl isothiocyanates by RP-HPLC in mustard powder extracts

Author:
Saqer Herzallah; Richard Holley
Source:
Lebensmittel-Wissenschaft + Technologie 2012 v.47 no.2 pp. 293-299
ISSN:
0023-6438
Subject:
boiling; thermal stability; detection limit; freeze drying; sulfates; allyl isothiocyanate; benzyl isothiocyanate; solvents; sinigrin; hydrogen; hydrolysis; reversed-phase high performance liquid chromatography; powders; thioglucosidase; wavelengths; autoclaving
Abstract:
... A reversed phase-high performance liquid chromatography method was developed to quantify sinigrin, sinalbin, allyl isothiocyanate and benzyl isothiocyanate present in aqueous and freeze-dried yellow and Oriental (brown) mustard extract samples using two pre-treatment methods (autoclaving, boiling) to prevent degradation by myrosinase. The lowest detection limits for sinigrin and sinalbin were 0.05 ...
DOI:
10.1016/j.lwt.2012.01.022

93. Erucin and Benzyl Isothiocyanate Suppress Growth of Late Stage Primary Human Ovarian Carcinoma Cells and Telomerase Activity In Vitro

Author:
Evelyn Lamy; Dewi Oey; Florian Eißmann; Corinna Herz; Karsten Münstedt; Hans‐Rudolf Tinneberg; Volker Mersch‐Sundermann
Source:
Phytotherapy research 2013 v.27 no.7 pp. 1036-1041
ISSN:
0951-418X
Subject:
B-lymphocytes; DNA fragmentation; acetylcysteine; antioxidants; apoptosis; ascites; benzyl isothiocyanate; cell lines; cytotoxicity; death; drug therapy; growth retardation; humans; mitochondrial membrane; neoplasm cells; ovarian neoplasms; patients; phytotherapy; pretreatment; research; staining; telomerase; thiols; viability
Abstract:
... In the present study we analysed the effects of isothiocyanates (ITCs) ‐ plant‐derived sulphur‐containing constituents known for their potential chemotherapeutic activity ‐ on growth inhibition and programmed death in primary ovarian carcinoma cells from ascites of human patients. Twenty‐four hour exposure of carcinoma cells to 5–50 μM erucin or benzyl ITC led to a concentration‐dependent viabilit ...
DOI:
10.1002/ptr.4798

94. Stability of Benzylic-Type Isothiocyanates in Hydrodistillation-Mimicking Conditions

Author:
De Nicola Gina R.; Montaut Sabine; Rollin Patrick; Nyegue Maximilienne; Menut Chantal; Iori Renato; Tatibouët Arnaud
Source:
Journal of agricultural and food chemistry 2013 v.61 no.1 pp. 137-142
ISSN:
1520-5118
Subject:
Pentadiplandra; alcohols; benzyl isothiocyanate; essential oils; glucosinolates; high performance liquid chromatography; roots
Abstract:
... Pentadiplandra brazzeana Baillon (Pentadiplandraceae) is known to contain benzyl-, 3-methoxybenzyl-, 4-methoxybenzyl-, 3,4-dimethoxybenzyl-, and indole-type glucosinolates, and the essential oil obtained from its roots is mainly constituted of benzyl isothiocyanate and benzyl cyanide. In a previous study by the authors, it was surmised that partial hydrolytic degradation of 4-methoxybenzyl isothio ...
DOI:
10.1021/jf3041534
PubMed:
23214966

95. Antimicrobial volatile glucosinolate autolysis products from Hornungia petraea (L.) Rchb. (Brassicaceae)

Author:
Niko S. Radulović; Milan S. Dekić; Zorica Z. Stojanović-Radić
Source:
Phytochemistry letters 2012 v.5 no.2 pp. 351-357
ISSN:
1874-3900
Subject:
Aspergillus fumigatus; Brassicaceae; Candida albicans; Micrococcus luteus; antibacterial properties; antifungal properties; autolysis; benzyl isothiocyanate; gas chromatography-mass spectrometry; glucosinolates; minimum inhibitory concentration; mustard oil; plant tissues; secondary metabolites
Abstract:
... Plant samples of Hornungia petraea were analyzed for glucosinolate (GLS) autolysis metabolites for the first time. GC–MS analysis of the autolysate and the synthesis of a series (12 compounds) of possible glucosinolate breakdown products revealed/corroborated the presence of glucoaubrietin, glucolimnanthin, glucolepigramin and glucotropaeolin in this species as the most likely “mustard oil” precur ...
DOI:
10.1016/j.phytol.2012.02.017

96. Assessment of the protective effects of selected dietary anticarcinogens against DNA damage and cytogenetic effects induced by benzo[a]pyrene in C57BL/6J mice

Author:
Dobrosława Gradecka-Meesters; Jadwiga Palus; Gabriela Prochazka; Dan Segerbäck; Elżbieta Dziubałtowska; Natalia Kotova; Dag Jenssen; Joanna Arkusz; Cecilia Lundin; Elisabet Vikström; Konrad Rydzyński; Robert Nilsson; Maciej Stępnik
Source:
Food and chemical toxicology 2011 v.49 no.8 pp. 1674-1683
ISSN:
0278-6915
Subject:
DNA adducts; DNA damage; benzo(a)pyrene; benzyl isothiocyanate; bone marrow; cytogenetics; ellagic acid; erythrocytes; flow cytometry; leukocytes; liver; mice; oral administration; protective effect; toxicology
Abstract:
... The protective action in C57BL/6J mice from orally administered ellagic acid (EA), benzyl isothiocyanate (BITC), an extract of epigallocatechins (Tegreen®) as well as chlorophyllin (CHL) against benzo[a]pyrene (B[a]P)-induced DNA damage and cytogenetic effects was investigated. In pilot experiment the comet assay indicated protective effects for all compounds, while such activity was confined to E ...
DOI:
10.1016/j.fct.2011.02.021

97. Contact and fumigant toxicity of Armoracia rusticana essential oil, allyl isothiocyanate and related compounds to Dermatophagoides farinae

Author:
Yeon‐Kyeong Yun; Hyun‐Kyung Kim; Jun‐Ran Kim; Kumnara Hwang; Young‐Joon Ahn
Source:
Pest management science 2012 v.68 no.5 pp. 788-794
ISSN:
1526-498X
Subject:
Armoracia rusticana; Dermatophagoides farinae; Glycyphagus domesticus; allyl isothiocyanate; benzyl benzoate; benzyl isothiocyanate; bioassays; chemical structure; containers; dibutyl phthalate; dust control; essential oils; fumigants; horseradish; lethal concentration 50; mortality; oils; toxicity; vapors
Abstract:
... BACKGROUND: The toxicity to adult Dermatophagoides farinae of allyl isothiocyanate identified in horseradish, Armoracia rusticana, oil and another 27 organic isothiocyanates was evaluated using contact + fumigant and vapour‐phase mortality bioassays. Results were compared with those of two conventional acaricides, benzyl benzoate and dibutyl phthalate. RESULTS: Horseradish oil (24 h LC50, 1.54 µg ...
DOI:
10.1002/ps.2327
PubMed:
22102526

98. Cytotoxicity of Benzyl Isothiocyanate in Normal Renal Proximal Tubular Cells and Its Modulation by Glutathione

Author:
Abe Naomi; Okuhira Masashi; Tsutsui Chiharu; Murata Yoshiyuki; Nakamura Yoshimasa
Source:
Journal of agricultural and food chemistry 2012 v.60 no.7 pp. 1887-1892
ISSN:
1520-5118
Subject:
benzyl isothiocyanate; cytotoxicity; glutathione; inhibitory concentration 50; necrosis; oxidative stress; swine
Abstract:
... In the present study, we examined the toxicity of benzyl ITC (BITC) and its urinary mercapturic acid metabolite (BITC-NAC), using a normal renal proximal tubular cell line, pig LLC-PK1. BITC increased cell death with an IC₅₀ value of about 7 μM, whereas the cytotoxic effect of BITC-NAC was five times weaker than that of BITC. We observed a significant necrosis of the compounds on LLC-PK1 cells wit ...
DOI:
10.1021/jf2052042
PubMed:
22296293

99. Enhancement of cisplatin cytotoxicity by benzyl isothiocyanate in HL-60 cells

Author:
Younghyun Lee; Yang Jee Kim; Young Joo Choi; Joong Won Lee; Sunyeong Lee; Hai Won Chung
Source:
Food and chemical toxicology 2012 v.50 no.7 pp. 2397-2406
ISSN:
0278-6915
Subject:
acetylcysteine; adverse effects; apoptosis; benzyl isothiocyanate; cell viability; chemoprevention; cisplatin; cytotoxicity; drug therapy; fruits; glutathione; humans; leukemia; lymphocytes; mitogen-activated protein kinase; neoplasm cells; reactive oxygen species; toxicology; vegetables
Abstract:
... Cis-diamminedichloroplatinum (II) (cisplatin) is one of the most widely used chemotherapeutic drugs, but its effectiveness is limited by tumor cell resistance and the severe side effects it causes. One strategy for overcoming this problem is the concomitant use of natural dietary compounds as therapeutic agents. Benzyl isothiocyanate (BITC) is a promising chemopreventive agent found in cruciferous ...
DOI:
10.1016/j.fct.2012.04.014

100. Interaction between Papaya meleira virus (PMeV) infection of papaya plants and Mediterranean fruit fly infestation of fruits

Author:
David dos Santos Martins; José Aires Ventura; Rita de Cássia Antunes Lima; Mark Paul Culik; Hélcio Costa; Paulo Sérgio Fiuza Ferreira
Source:
Crop protection 2012 v.36 no. pp. 7-10
ISSN:
0261-2194
Subject:
Ceratitis capitata; benzyl isothiocyanate; fruit flies; fruits; latex; maturity stage; papayas; pests; plant diseases and disorders; quarantine; ripening; viruses
Abstract:
... The Mediterranean fruit fly (Medfly), Ceratitis capitata (Wied.), is a pest of quarantine importance that usually only infests papaya fruits in advanced stages of maturity. However, some diseases, such as sticky disease of papaya plants, caused by the Papaya meleira virus (PMeV), break the natural resistance of fruits to fruit flies, enabling them to be infested while still unripe. Therefore, this ...
DOI:
10.1016/j.cropro.2012.01.001