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1. Inhibition of velvetleaf (Abutilon theophrasti) germination and growth by benzyl isothiocyanate, a natural toxicant

Author:
Wolf, R.B.; Spencer, G.F.; Kwolek, W.F.
Source:
Weed science 1984 v.32 no.5 pp. 612-615
ISSN:
0043-1745
Subject:
chemical concentration; phytotoxins; plant growth; weed control; germination; Glycine max; seeds; Zea mays; phytotoxicity; biological control agents; seedlings; soybeans; corn; benzyl isothiocyanate; Abutilon theophrasti; biological control; Carica papaya
Abstract:
... Imbibed seeds of A. theophrasti were placed in Petri dishes and treated with 0-6 × 10-4 M benzyl isothiocyanate (BITC) extracted from mature papaya seeds. At 6 × 10-4 M BITC germination was completely inhibited and after exposure to 4 × 10-4 M BITC for 4 days germination was only 28% of that in the absence of BITC. When 4 × 10-4 M BITC was applied to 3-day-old etiolated seedlings all died within 2 ...
Handle:
10113/26225

2. Changes in physiology, composition and sensory characteristics of Australian papaya during ripening

Author:
Wills, R.B.H.; Widjanarko, S.B.
Source:
Animal production science 1995 v.35 no.8 pp. 1173-1176
ISSN:
0816-1089
Subject:
postharvest treatment; bitterness; sensory evaluation; texture; carotenes; ethylene production; food quality; benzyl isothiocyanate; ripening; color; temperature; ascorbic acid
DOI:
10.1071/EA9951173

3. Role of ethylene in the production of sporophytes from Platycerium coronarium (Koenig) desv. frond and rhizome pieces cultured in vitro

Author:
Kwa, S.H.; Wee, Y.C.; Kumar, P.P.
Source:
Journal of plant growth regulation 1995 v.14 no.4 pp. 183-189
ISSN:
0721-7595
Subject:
ethylene; 1-aminocyclopropane-1-carboxylic acid; biosynthesis; Platycerium coronarium; dose response; explants; benzyl isothiocyanate; silver thiosulfate; salicylic acid; rhizomes; aminoethoxyvinylglycine; culture media; leaves
Abstract:
... The effect of ethylene on in vitro plant regeneration from frond and rhizome explants of Platycerium coronarium was investigated. Ethylene levels in the culture vessels increased with time, resulting in a decrease in the percentage of sporophytes produced. Addition of the ethylene action inhibitor silver thiosulfate resulted in an increase in the percentage of plants regenerated, indicating an inh ...
DOI:
10.1007/BF00204910

4. Chemical reactions of benzyl isothiocyanate with myoglobin

Author:
Kroll, J.; Rawel, H.
Source:
Journal of the science of food and agriculture 1996 v.72 no.3 pp. 376-384
ISSN:
0022-5142
Subject:
lysine; chemical reactions; myoglobin; fluorescence; benzyl isothiocyanate; tryptophan; thiourea; hydrophobicity; solubility; molecular weight
Abstract:
... The interaction of benzyl isothiocyanate (in concentrations of 2.5-250 mg benzyl-ITC g-1 protein) with myoglobin leads to the formation of derivatives which have been characterised in terms of their solubility, free epsilon amino groups, content of tryptophan and its quenching, as well as their molecular properties determined with electrophoretic and chromatographic methods. The reaction takes pla ...
DOI:
10.1002/(SICI)1097-0010(199611)72:3<376::AID-JSFA670>3.0.CO;2-Y

5. Aromatic plants of tropical Central Africa. XXIX. Benzyl isothiocyanate as major constituent of bark essential oil of Drypetes gossweileri S. Moore

Author:
Mve-Mba, C.E.; Menut, C.; Bessiere, J.M.; Lamaty, G.; Ekekang, L.N.; Denanganai, J.
Source:
Journal of essential oil research 1997 v.9 no.3 pp. 367-370
ISSN:
1041-2905
Subject:
Drypetes; essential oils; bark; benzyl isothiocyanate; cyanides; chemical constituents of plants; Central African Republic
Abstract:
... Essential oils obtained from bark samples of Drypetes gossweileri collected in Central African Republic and Gabon were analyzed by GC and GC/MS. Their chemical composition is particularly simple, with only three constituents: benzyl isothiocyanate (56-94%), accompanied by benzyl cyanide and benzaldehyde. ...
DOI:
10.1080/10412905.1997.10554264

6. Volatile constituents isolated from Capparis flexuosa of Brazil

Author:
Gramosa, N.V.; Lemos, T.L.G.; Braz-Filho, R.
Source:
Journal of essential oil research 1997 v.9 no.6 pp. 709-712
ISSN:
1041-2905
Subject:
Capparis; essential oils; volatile compounds; benzyl isothiocyanate; methyl isothiocyanate; leaves; bark; pods; chemical constituents of plants; Brazil
Abstract:
... Essential oil analysis of Capparis flexuosa (Capparaceae) from Brazil allowed us to characterize 3-methyl-3-buteneisothiocyanate, 4-methyl-4-pentenenitrile and butyl isothiocyanate. Structure determinations were established by spectroscopic analysis (IR, MS, NMR) and chemical derivatization. ...
DOI:
10.1080/10412905.1997.9700819

7. In vitro enzymatic digestion of benzyl- and phenylisothiocyanate-derivatized food proteins

Author:
Rawel, H.M.; Kroll, J.; Insa-Schroder.
Source:
Journal of agricultural and food chemistry 1998 v.46 no.12 pp. 5103-5109
ISSN:
0021-8561
Subject:
trypsin; myoglobin; chymotrypsin; proteolysis; benzyl isothiocyanate; physicochemical properties; legumin; hydrolysis; egg albumen; pepsin
Abstract:
... The interactions of different isothiocyanates (ITCs, benzyl- and phenyl-ITC) with selected food proteins such as egg white proteins, myoglobin, and legumin have been investigated. The first aspect summarizes the changes in physicochemical properties of the derivatized proteins such as a decrease in solubility and in the amount of free epsilon-amino groups coupled with an increase in the hydrophobi ...
DOI:
10.1021/jf980244r

8. Simultaneous Treatment With Benzyl Isothiocyanate, a Strong Bladder Promoter, Inhibits Rat Urinary Bladder Carcinogenesis by N-Butyl-N-(4-Hydroxybutyl)Nitrosamine

Author:
Okazaki, Kazushi; Yamagishi, Megumi; Son, Hwa-Young; Imazawa, Takayoshi; Furukawa, Fumio; Nakamura, Hideaki; Nishikawa, Akiyoshi; Masegi, Toshiaki; Hirose, Masao
Source:
Nutrition and cancer 2002 v.42 no.2 pp. 211-216
ISSN:
0163-5581
Subject:
benzyl isothiocyanate; bladder; carcinogenesis; carcinogenicity; carcinoma; chemoprevention; diet; drinking water; hyperplasia; necropsy; papilloma; rats; urinary bladder neoplasms
Abstract:
... Effects of benzyl isothiocyanate (BITC) on urinary bladder carcinogenesis were examined in rats simultaneously treated with N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN). Groups of 20 6-wk-old Fischer 344 male rats were given 10, 100, or 1,000 ppm BITC in the diet or a basal diet with 50 ppm BBN in the drinking water for 40 wk and then killed for autopsy. Additional groups consisting of 10 or 9 rats ...
DOI:
10.1207/S15327914NC422_10
PubMed:
12416262

9. The composition of the root oil of Salvadora persica L

Author:
Bader, A.; Flamini, G.; Cioni, P.L.; Morelli, I.
Source:
Journal of essential oil research 2002 v.14 no.2 pp. 128-129
ISSN:
1041-2905
Subject:
Salvadora persica; essential oils; roots; limonene; alpha-pinene; benzyl isothiocyanate; chemical constituents of plants; Jordan
Abstract:
... The composition of the essential oil from the roots of Salvadora persica L. (Salvadoraceae), collected in Jordan, was analyzed by GC and GC/MS. Seventeen compounds were detected, the main constituents being benzyl isothiocyanate (70%), limonene (9.4%) and alpha-pinene (8.7%). ...
DOI:
10.1080/10412905.2002.9699795

10. Cross-enhancement: enhanced biodegradation of isothiocyanates in soils previously treated with metham sodium

Author:
Warton, Ben; Matthiessen, John N.; Shackleton, Mark A.
Source:
Soil biology & biochemistry 2003 v.35 no.8 pp. 1123-1127
ISSN:
0038-0717
Subject:
biofumigation; plant tissues; toxicity; Brassica; methyl isothiocyanate; benzyl isothiocyanate; soil; sinigrin; biodegradation; metam; sodium; insects; mortality
Abstract:
... Rates of degradation of 2-propenyl isothiocyanate (PrITC), benzyl isothiocyanate (BeITC) and 2-phenylethyl isothiocyanate (2-PeITC) in a soil known to biodegrade methyl isothiocyanate (MITC) at an accelerated rate, but never previously exposed to the other ITCs, were higher (persistence in soil increased by 1150, 80 and 100%, respectively,) than in a similar non-degrading soil. The rate of degrada ...
DOI:
10.1016/S0038-0717(03)00164-0

11. Enhancement of Glutathione and γ-Glutamylcysteine Synthetase, the Rate Limiting Enzyme of Glutathione Synthesis, by Chemoprotective Plant-Derived Food and Beverage

Author:
Scharf, Gerlinde; Prustomersky, Sonia; Knasmüller, Siegfried; Schulte-Hermann, Rolf; Huber, Wolfgang W.
Source:
Nutrition and cancer 2003 v.45 no.1 pp. 74-83
ISSN:
0163-5581
Subject:
antioxidants; benzyl isothiocyanate; beverages; chemoprevention; diallyl sulfides; glutamate-cysteine ligase; glutathione; hepatoma; human cell lines; humans; liver; metabolism; models; plant-based foods; quercetin
Abstract:
... Glutathione (GSH) is an important antioxidant and cofactor of detoxifying metabolism. Therefore, elevation of GSH as achieved by inducing γ-glutamylcysteine synthetase (GCS), the limiting enzyme of GSH synthesis, may contribute to chemoprevention against cancer. In previous animal studies, increases in GCS were mainly found in liver and other organs that are not easily accessible in humans. Thus, ...
DOI:
10.1207/S15327914NC4501_9

12. Dietary Isothiocyanates Modify Mitochondrial Functions through Their Electrophilic Reaction

Author:
Makiko KAWAKAMI; Nobuhiro HARADA; Mio HIRATSUKA; Kiyoshi KAWAI; Yoshimasa NAKAMURA
Source:
Bioscience, biotechnology, and biochemistry 2005 v.69 no.12 pp. 2439-2444
ISSN:
1347-6947
Subject:
Lewis acids; benzyl isothiocyanate; biotechnology; cyclosporine; membrane permeability; mitochondria; thiols
Abstract:
... We found that both benzyl isothiocyanate (ITC) and phenyl ITC inhibited respiration in the mitochondria in an electrophilic reaction-dependent manner. ITC-induced mitochondrial swelling and cytochrome c release were prevented by cyclosporin A, indicating that they are mediated through the ITC moiety-dependent reaction to critical thiol groups for the opening of membrane permeability transition-dep ...
DOI:
10.1271/bbb.69.2439

13. Effect of benzyl isothiocyanate on tomato fruit infection development by Alternaria alternata

Author:
Troncoso-Rojas, R.; Sanchez-Estrada, A.; Ruelas, C.; Garcia, H.S.; Tiznado-Hernandez, M.E.
Source:
Journal of the science of food and agriculture 2005 v.85 no.9 pp. 1427-1434
ISSN:
0022-5142
Subject:
tomatoes; postharvest diseases; Alternaria alternata; benzyl isothiocyanate; disease control; antifungal properties; postharvest treatment; postharvest physiology; cell respiration; ethylene production; food storage
Abstract:
... Benzyl isothiocyanate (BITC) is known to be a strong antifungal compound in vitro against different fungi. The effectiveness of benzyl isothiocyanate to control Alternaria alternata growth in vitro and in vivo was tested. BITC in vitro activity was evaluated in A alternata growing on potato dextrose agar and exposed to 0.025, 0.05, 0.1, 0.2 or 0.4 mg ml(-1). In vivo activity was evaluated by expos ...
DOI:
10.1002/jsfa.2129

14. Effect of isothiocyanates on nuclear accumulation of NFkappa-B, Nrf2, and thioredoxin in Caco-2 cells

Author:
Jakubikova, J.; Sedlak, J.; Bod'o, J.; Bao, Y.
Source:
Journal of agricultural and food chemistry 2006 v.54 no.5 pp. 1656-1662
ISSN:
0021-8561
Subject:
chemical concentration; transcription factors; glutathione; bioactive properties; humans; animal proteins; reactive oxygen species; adenocarcinoma; benzyl isothiocyanate; cell nucleus; cell lines; small intestine
Abstract:
... Early effects (only 1 h of exposure) of three isothiocyanates (benzyl, phenylethyl, and sulforaphane) on nuclear accumulation of thioredoxin, APE/Ref-1, and transcription factors NF-kappaB and Nrf2, as well as production of reactive oxygen species (ROS) and reduced glutathione levels were examined in human adenocarcinoma Caco-2 cells. Nuclear increase of NF-kappaB, Nrf2, and thioredoxin contents w ...
DOI:
10.1021/jf052717h

15. Volatile constituents of the seeds, roots and non-flowering aerial parts of Lepidium satvium L

Author:
Afsharypuor, S.; Hadi, M.E.
Source:
Journal of essential oil research 2006 v.18 no.5 pp. 495-496
ISSN:
1041-2905
Subject:
chemical composition; cultivars; salads; seed extracts; seeds; monoterpenoids; essential oils; medicinal plants; volatile organic compounds; benzyl isothiocyanate; aerial parts; alkanes; vegetables; Lepidium sativum; roots; Iran
Abstract:
... Volatile constituents of the seeds, roots and dried non-flowering aerial parts of Lepidium sativum L. collected after hydrolysis were analyzed by GC and GC/MS. The main volatile constituents of the seeds were phenylacetonitrile (52.9%), benzyl isothiocyanate (26.2%) and 1,8-cineole (12.3%), while the main volatile constituents of the roots and the non-flowering aerial parts were benzyl isothiocyan ...
DOI:
10.1080/10412905.2006.9699151

16. Modifying Effects of Dietary Factors on (−)-Epigallocatechin-3-gallate-induced Pro-matrix Metalloproteinase-7 Production in HT-29 Human Colorectal Cancer Cells

Author:
Mihye KIM; Akira MURAKAMI; Hajime OHIGASHI
Source:
Bioscience, biotechnology, and biochemistry 2007 v.71 no.10 pp. 2442-2450
ISSN:
1347-6947
Subject:
acetylcysteine; benzyl isothiocyanate; biotechnology; chemoprevention; colorectal neoplasms; curcumin; epigallocatechin gallate; gallic acid; gene expression; green tea; humans; hydrogen peroxide; metalloproteinases
Abstract:
... (−)-Epigallocatechin-3-gallate (EGCG), one of the main constituents of green tea, has been reported to function as an antioxidant with chemopreventive potential. In contrast, we have recently reported that EGCG enhanced pro-matrix metalloproteinase (MMP)-7 in HT-29 human colon cancer cells via spontaneous superoxide generation. In the present study, we examined the effects of dietary antioxidants ...
DOI:
10.1271/bbb.70213

17. Papaya Seed Represents a Rich Source of Biologically Active Isothiocyanate

Author:
Nakamura, Y.; Yoshimoto, M.; Murata, Y.; Shimoishi, Y.; Asai, Y.; Park, E.Y.; Sato, K.
Source:
Journal of agricultural and food chemistry 2007 v.55 no.11 pp. 4407-4413
ISSN:
0021-8561
Subject:
functional foods; chemical composition; papayas; seeds; anticarcinogenic activity; glucosinolates; benzyl isothiocyanate; fruit pulp; thioglucosidase; chemical constituents of plants; superoxide anion; antioxidant activity; apoptosis; Carica papaya
Abstract:
... In the present study, papaya (Carica papaya) seed and edible pulp were carefully separated and then the contents of benzyl isothiocyanate and the corresponding glucosinolate (benzyl glucosinolate, glucotropaeolin) quantified in each part. The papaya seed with myrosinase inactivation contained >1 mmol of benzyl glucosinolate in 100 g of fresh seed. This content is equivalent to that of Karami daiko ...
DOI:
10.1021/jf070159w

18. A new method for collecting isothiocyanates released from plant residues incorporated in soil

Author:
Papadopoulos, A.; Alderson, P.
Source:
Annals of applied biology 2007 v.151 no.1 pp. 61-65
ISSN:
0003-4746
Subject:
plant tissues; cultivars; freeze drying; molecular weight; Cleome hassleriana; composts; plant residues; allyl isothiocyanate; methyl isothiocyanate; benzyl isothiocyanate; soil; hydrolysis; Tropaeolum majus; mass spectrometry; gas chromatography; Brassica carinata; databases
Abstract:
... A method was developed for collecting isothiocyanates (ITCs) from compost and their detection by gas chromatography-mass spectrometry. Identification of the compounds was based on retention time, molecular weight and previously published information (Wiley Database, Hewlett Packard ChemStation Library). The release of ITCs from freeze-dried plant material of Brassica carinata, Cleome spinosa and T ...
DOI:
10.1111/j.1744-7348.2007.00149.x

19. (−)-Epigallocatechin-3-gallate Potentiates the Cytotoxicity Induced by Benzyl Isothiocyanate and Hydrogen Peroxide in Human Jurkat T Lymphocytes

Author:
Haitao WU; Tomoko YOKOYAMA; Beiwei ZHU; Yasuaki SHIMOISHI; Yoshiyuki MURATA; Yoshimasa NAKAMURA
Source:
Bioscience, biotechnology, and biochemistry 2008 v.72 no.11 pp. 3034-3037
ISSN:
1347-6947
Subject:
apoptosis; benzyl isothiocyanate; biotechnology; cytotoxicity; epigallocatechin gallate; humans; hydrogen peroxide; mitogen-activated protein kinase
Abstract:
... (−)-Epigallocatechin-3-gallate (EGCG)-induced apoptosis was along both the extracellular signal-regulated protein kinase (ERK) and c-jun N-terminal kinase (JNK) pathways in Jurkat cells. Co-treatment with EGCG potentiated the cytotoxicity induced by benzyl isothiocyanate (BITC) and H₂O₂, both being inhibited by ERK and JNK inhibitors. These results suggest the significant role of mitogen-activated ...
DOI:
10.1271/bbb.80422

20. Benzyl isothiocyanate inhibits metalloproteinase-2/-9 expression by suppressing the mitogen-activated protein kinase in SK-Hep1 human hepatoma cells

Author:
Hwang, E.S.; Lee, H.J.
Source:
Food and chemical toxicology 2008 v.46 no.7 pp. 2358-2364
ISSN:
0278-6915
Subject:
hepatoma; cell lines; benzyl isothiocyanate; metalloproteinases; mitogen-activated protein kinase; enzyme inhibition; cell proliferation; metastasis; dose response; protein synthesis; messenger RNA; enzyme activity; biochemical pathways; gene expression; human diseases; human nutrition
DOI:
10.1016/j.fct.2008.03.016
PubMed:
18502015

21. The invasive plant Alliaria petiolata (garlic mustard) inhibits ectomycorrhizal fungi in its introduced range

Author:
Wolfe, Benjamin E.; Rodgers, Vikki L.; Stinson, Kristina A.; Pringle, Anne
Source:
Journal of ecology 2008 v.96 no.4 pp. 777-783
ISSN:
0022-0477
Subject:
Alliaria petiolata; Impatiens capensis; Pinus strobus; benzyl isothiocyanate; biogeochemical cycles; biomass; conifers; ectomycorrhizae; forest ecosystems; forests; fungal communities; greenhouse experimentation; herbaceous plants; indigenous species; invasive species; mycorrhizal fungi; seedlings; soil; trees; New England region; United States
Abstract:
... 1. Ectomycorrhizal (EM) fungi play key roles in forest ecosystems, but the potential effects of invasive plants on EM fungal communities have not been assessed. In this study, we tested whether the non-mycorrhizal herbaceous plant Alliaria petiolata (garlic mustard) can alter the abundance of EM fungal communities in North America. 2. In three forests in New England, USA, we compared EM root tip a ...
DOI:
10.1111/j.1365-2745.2008.01389.x

22. Behavioural response of Meloidogyne incognita to benzyl isothiocyanate

Author:
Inga A. Zasada; Edward P. Masler; John M. Halbrendt; Stephen T. Rogers
Source:
Nematology v.11 no.4 pp. 603-610
ISSN:
1388-5545
Subject:
Meloidogyne incognita; root-knot nematodes; benzyl isothiocyanate; animal behavior; locomotion; nematode larvae; nematode control; Glycine max; soybeans
Abstract:
... The breakdown of brassicaceous plant material produces nematotoxic isothiocyanates (ITCs). However, after the incorporation of brassicaceous plant material into soil, many nematodes are likely to be exposed only to sublethal concentrations of these compounds. Although unknown, the effect of these low ITC concentrations on the behaviour of plant-parasitic nematodes could play a role in nematode sup ...
Handle:
10113/50231
DOI:
10.1163/138855409X12465362560674

23. Degradation and sorption of 2-propenyl and benzyl isothiocyanate in soil

Author:
Gimsing, Anne L.; Strobel, Bjarne W.; Hansen, Hans C.B.
Source:
Environmental toxicology and chemistry 2009 v.28 no.6 pp. 1178-1184
ISSN:
0730-7268
Subject:
degradation; benzyl isothiocyanate; glucosinolates; pesticides; chemical constituents of plants; hydrolysis; soil organic matter; Brassica; A horizons; B horizons; sandy soils; loam soils
Abstract:
... Isothiocyanates of natural origin produced by the hydrolysis of plant-produced glucosinolates have the potential to control soil pests, but getting sufficiently high isothiocyanate concentrations in soil is difficult. Furthermore, the isothiocyanates have proven toxic to a wide range of organisms and hence may also harm nontarget organisms. Knowledge of the sorption and degradation of the isothioc ...
DOI:
10.1897/08-516.1
PubMed:
19191470

24. Phytochemical Induction of Cell Cycle Arrest by Glutathione Oxidation and Reversal by N-Acetylcysteine in Human Colon Carcinoma Cells

Author:
Odom, R.Y.; Dansby, M.Y.; Rollins-Hairston, A.M.; Jackson, K.M.; Kirlin, W.G.
Source:
Nutrition and cancer 2009 v.61 no.3 pp. 332-339
ISSN:
0163-5581
Subject:
human diseases; redox potential; glutathione; cell growth; acetylcysteine; phytochemicals; disease prevention; benzyl isothiocyanate; lycopene; cell proliferation; oxidation; colorectal neoplasms; cell cycle
Abstract:
... Cancer prevention by dietary phytochemicals has been shown to involve decreased cell proliferation and cell cycle arrest. However, there is limited understanding of the mechanisms involved. Previously, we have shown that a common effect of phytochemicals investigated is to oxidize the intracellular glutathione (GSH) pool. Therefore, the objective of this study was to evaluate whether changes in th ...
DOI:
10.1080/01635580802549982
PubMed:
19373606
PubMed Central:
PMC2749979

25. Postharvest treatment of isothiocyanates to control Alternaria rot in netted melon

Author:
Troncoso-Rojas, Rosalba; Corral-Acosta, Yolanda; Sanchez-Estrada, Alberto; Garcia-Estrada, Raymundo; Aguilar-Valenzuela, Alfonso; Ojeda-Contreras, Javier; Tiznado-Hernandez, Martin Ernesto
Source:
Phytoparasitica 2009 v.37 no.5 pp. 445-451
ISSN:
0334-2123
Subject:
cantaloupes; total soluble solids; postharvest diseases; allyl isothiocyanate; disease control; benzyl isothiocyanate; food quality; Cucumis melo subsp. melo var. cantalupo; Alternaria alternata; titratable acidity; pH; firmness; captan; application rate; Mexico
DOI:
10.1007/s12600-009-0062-3

26. The antimicrobial effects of glucosinolates and their respective enzymatic hydrolysis products on bacteria isolated from the human intestinal tract

Author:
Aires, A.; Mota, V.R.; Saavedra, M.J.; Rosa, E.A.S.; Bennett, R.N.
Source:
Journal of applied microbiology 2009 v.106 no.6 pp. 2086-2095
ISSN:
1364-5072
Subject:
Gram-negative bacteria; Gram-positive bacteria; amines; antibacterial properties; benzyl isothiocyanate; bioassays; diet; enzymatic hydrolysis; glucosinolates; growth retardation; humans; hydrolysis; in vitro studies; infectious diseases; intestines; nitriles; pathogens
Abstract:
... The aim of the study was to evaluate the in vitro antibacterial activity of glucosinolates and their enzymatic hydrolysis product against bacteria isolated from the human intestinal tract. Using a disc diffusion bioassay, different doses of intact glucosinolates and their corresponding hydrolysis products were tested. There were clear structure-activity and concentration differences with respect t ...
DOI:
10.1111/j.1365-2672.2009.04180.x
PubMed:
19291240

27. Benzyl Isothiocyanate (BITC) Inhibits Migration and Invasion of Human Colon Cancer HT29 Cells by Inhibiting Matrix Metalloproteinase-2/-9 and Urokinase Plasminogen (uPA) through PKC and MAPK Signaling Pathway

Author:
Lai, Kuang-Chi; Huang, An-Cheng; Hsu, Shu-Chun; Kuo, Chao-Lin; Yang, Jai-Sing; Wu, Shin-Hwar; Chung, Jing-Gung
Source:
Journal of agricultural and food chemistry 2010 v.58 no.5 pp. 2935-2942
ISSN:
0021-8561
Subject:
functional foods; vegetables; human cell lines; metalloproteinases; signal transduction; benzyl isothiocyanate; biochemical pathways; protein kinase C; plasminogen; colorectal neoplasms; u-plasminogen activator; mitogen-activated protein kinase; anticarcinogenic activity
Abstract:
... Benzyl isothiocyanate (BITC), a component of dietary cruciferous vegetables, has antioxidant and anticancer properties. In this study, we show for the first time the antimetastatic effects of BITC in human colon cancer HT29 cells. BITC had an inhibitory effect on cell migration and invasion. Protein levels of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9), and urokinase-pla ...
DOI:
10.1021/jf9036694
PubMed:
20136087

28. Effects of benzyl isothiocyanate on the reproduction of Meloidogyne incognita on Glycine max and Capsicum annuum

Author:
Edward P. Masler; Inga A. Zasada; Sandra Sardanelli; Stephen T. Rogers; John M. Halbrendt
Source:
Nematology v.12 no.5 pp. 693-699
ISSN:
1388-5545
Subject:
Meloidogyne incognita; Capsicum annuum; Glycine max; soybeans; paprika; benzyl isothiocyanate; root-knot nematodes; nematode control; nematode larvae; egg masses; hatching; correlation; phytotoxicity; roots; shoots; plant health; host plants
Abstract:
... Reproduction of Meloidogyne incognita on either Capsicum annuum or Glycine max was suppressed when infective second-stage juveniles (J2) were exposed to 0.03 mM benzyl isothiocyanate (BITC) for 2 h prior to inoculation of the host. Infectivity as rated by a gall index was significantly reduced on both G. max and C. annuum. In C. annuum, nematode egg masses were recovered from 48% of the plants ino ...
Handle:
10113/49618
DOI:
10.1163/138855409X12595802046375

29. Evolution of volatile compounds in papaya wine fermented with three Williopsis saturnus yeasts

Author:
Lee, Pin-Rou; Ong, Yuen-Ling; Yu, Bin; Curran, Philip; Liu, Shao-Quan
Source:
International journal of food science & technology 2010 v.45 no.10 pp. 2032-2041
ISSN:
0950-5423
Subject:
alcohols; benzaldehyde; benzyl isothiocyanate; brix; butyric acid; fermentation; fructose; glucose; juices; odor compounds; pH; wines; yeasts
Abstract:
... The changes in volatile compounds during papaya juice fermentation with three Williopsis saturnus yeasts were investigated in this study. Time-course papaya juice fermentations were carried out using three Williopsis saturnus yeasts: W. saturnus var. mrakii NCYC2251, W. saturnus var. saturnus NCYC22 and W. saturnus var. sargentensis NCYC2727. Changes in yeast cell population, Brix and pH were simi ...
DOI:
10.1111/j.1365-2621.2010.02369.x

30. Immunochemical Detection of Food-Derived Isothiocyanate as a Lysine Conjugate

Author:
Toshiyuki NAKAMURA; Noritoshi KITAMOTO; Toshihiko OSAWA; Yoji KATO
Source:
Bioscience, biotechnology, and biochemistry 2010 v.74 no.3 pp. 536-540
ISSN:
1347-6947
Subject:
Carica papaya; Eutrema japonica; allyl isothiocyanate; benzyl isothiocyanate; biotechnology; bovine serum albumin; dose response; immunochemistry; lysine; monoclonal antibodies
Abstract:
... In a previous study we prepared monoclonal antibody against allyl isothiocyanate (AITC)-modified lysine (Lys), and found that AITC reacted with Lys under physiological conditions in vitro (T. Nakamura et al., Chem. Res. Toxicol., 22, 536–542 (2009)). In the present study, antibodies against benzyl isothiocyanate (ITC), 6-methylsulfinylhexyl ITC and phenethyl ITC modified protein were prepared, and ...
DOI:
10.1271/bbb.90728

31. Kinetics of volatile organic compounds during papaya juice fermentation by three commercial wine yeasts

Author:
Lee, Pin Rou; Yu, Bin; Curran, Philip; Liu, Shao-Quan
Source:
Nutrition and food science 2010 v.40 no.6 pp. 566-580
ISSN:
0034-6659
Subject:
Saccharomyces cerevisiae; absorbance; acetaldehyde; acetoin; alcohols; benzaldehyde; benzyl isothiocyanate; brix; fatty acids; fermentation; gas chromatography; ionization; juices; microextraction; nitrogen; pH; volatile organic compounds; wine yeasts; wines
Abstract:
... Purpose – The purpose of this paper is to assess the potential of papaya juice fermentation and to evaluate the kinetic changes of volatile organic compounds (VOCs) produced during fermentation by three commercial wine yeasts. Design/methodology/approach – Laboratory-scale fermentations were carried out in papaya juice using three commercial wine yeasts Saccharomyces cerevisiae var. bayanus strain ...
DOI:
10.1108/00346651011090374

32. Quality and volatile attributes of attached and detached ‘Pluk Mai Lie' papaya during fruit ripening

Author:
Fuggate, P.; Wongs-Aree, C.; Noichinda, S.; Kanlayanarat, S.
Source:
Scientia horticulturae 2010 v.126 no.2 pp. 120-129
ISSN:
0304-4238
Subject:
papayas; ethylene; total soluble solids; postharvest physiology; odors; Carica papaya; benzyl isothiocyanate; fruit quality; titratable acidity; alcohol dehydrogenase; volatile compounds; cultivars; firmness; ethanol; methanol; ethylene production; ripening; color; mesocarp
Abstract:
... The ‘Pluk Mai Lie' papaya (Carica papaya L.) is a promising cultivated fruit for use in fresh and processed products due to its firm flesh, but the aroma it releases is flat. Changes in quality and volatile profiles were analyzed during on- and off-tree fruit ripening. Detached fruit ripened faster than attached fruit, accumulating high internal ethylene levels. Aside from peel color, which was re ...
DOI:
10.1016/j.scienta.2010.06.019

33. Study on the compositional differences between transgenic and non-transgenic papaya (Carica papaya L.)

Author:
Jiao, Zhe; Deng, Jianchao; Li, Gongke; Zhang, Zhuomin; Cai, Zongwei
Source:
Journal of food composition and analysis 2010 v.23 no.6 pp. 640-647
ISSN:
0889-1575
Subject:
Carica papaya; Papaya ringspot virus; alkaloids; analysis of variance; ascorbic acid; benzyl isothiocyanate; beta-carotene; genes; genetically modified foods; high performance liquid chromatography; natural toxicants; nutrients; papayas; principal component analysis; volatile organic compounds
Abstract:
... Transgenic papaya (Carica papaya L.) was produced with the introduction of replicase (rep) gene for resistance to papaya ringspot virus (PRSV). In order to investigate the potential unintended compositional changes in transgenic papaya, profiles of volatile organic compounds (VOCs), sugar/polyals, organic acids, carotenoids and alkaloids in transgenic and non-transgenic papaya were obtained respec ...
DOI:
10.1016/j.jfca.2010.03.004

34. The toxic effects of benzyl glucosinolate and its hydrolysis product, the biofumigant benzyl isothiocyanate, to Folsomia fimetaria

Author:
John Jensen; Bjarne Styrishave; Anne Louise Gimsing; Hans Christian Bruun Hansen
Source:
Environmental toxicology and chemistry 2010 v.29 no.2 pp. 359-364
ISSN:
0730-7268
Subject:
Folsomia; Nematoda; adults; agricultural soils; benzyl isothiocyanate; biofumigation; death; ecotoxicology; glucosinolates; half life; hydrolysis; juveniles; lethal concentration 50; median effective concentration; mortality; reproduction; toxicity
Abstract:
... Natural isothiocyanates (ITCs) are toxic to a range of pathogenic soil‐living species, including nematodes and fungi, and can thus be used as natural fumigants called biofumigants. Natural isothiocyanates are hydrolysis products of glucosinolates (GSLs) released from plants after cell rupture. The study investigated the toxic effects of benzyl‐GSL and its hydrolysis product benzyl‐ITC on the sprin ...
DOI:
10.1002/etc.33

35. Assessment of the protective effects of selected dietary anticarcinogens against DNA damage and cytogenetic effects induced by benzo[a]pyrene in C57BL/6J mice

Author:
Dobrosława Gradecka-Meesters; Jadwiga Palus; Gabriela Prochazka; Dan Segerbäck; Elżbieta Dziubałtowska; Natalia Kotova; Dag Jenssen; Joanna Arkusz; Cecilia Lundin; Elisabet Vikström; Konrad Rydzyński; Robert Nilsson; Maciej Stępnik
Source:
Food and chemical toxicology 2011 v.49 no.8 pp. 1674-1683
ISSN:
0278-6915
Subject:
DNA adducts; DNA damage; benzo(a)pyrene; benzyl isothiocyanate; bone marrow; cytogenetics; ellagic acid; erythrocytes; flow cytometry; leukocytes; liver; mice; oral administration; protective effect; toxicology
Abstract:
... The protective action in C57BL/6J mice from orally administered ellagic acid (EA), benzyl isothiocyanate (BITC), an extract of epigallocatechins (Tegreen®) as well as chlorophyllin (CHL) against benzo[a]pyrene (B[a]P)-induced DNA damage and cytogenetic effects was investigated. In pilot experiment the comet assay indicated protective effects for all compounds, while such activity was confined to E ...
DOI:
10.1016/j.fct.2011.02.021

36. Characteristics of papaya seed oils obtained by extrusion–expelling processes

Author:
Wei‐Ju Lee; Min‐Hsiung Lee; Nan‐Wei Su
Source:
Journal of the science of food and agriculture 2011 v.91 no.13 pp. 2348-2354
ISSN:
0022-5142
Subject:
benzyl isothiocyanate; food industry; fruits; functional foods; olive oil; papayas; polyunsaturated fatty acids; processing technology; seed oils; seeds
Abstract:
... BACKGROUND: In general, about 300 g kg−1 of the weight of papaya fruits appears as waste materials during processing, including a considerable amount of papaya seeds. To make a more efficient use of papaya, it is worth investigating the utilization of the seeds. The aim of this study was to comprehensively assess the lipid characteristics of papaya seed oil obtained by expelling processes. RESULTS ...
DOI:
10.1002/jsfa.4466
PubMed:
21590777

37. Effectiveness of different solid-phase microextraction fibres for differentiation of selected Madeira island fruits based on their volatile metabolite profile—Identification of novel compounds

Author:
João Pereira; Jorge Pereira; José S. Câmara
Source:
Talanta 2011 v.83 no.3 pp. 899-906
ISSN:
0039-9140
Subject:
Actinidia deliciosa; Carica papaya; Citrus limon; Prunus angustifolia; acetic acid; benzyl isothiocyanate; butyrates; commercialization; fruits; furfural; gamma-terpinene; gas chromatography; headspace analysis; kiwifruit; lemons; linalool; mass spectrometry; metabolites; odors; papayas; plums; polyacrylic acid; pulp; silicone; solid phase microextraction; volatile compounds; Madeira Islands
Abstract:
... A headspace solid-phase microextraction (HS-SPME) procedure based on five commercialised fibres (85μm polyacrylate – PA, 100μm polydimethylsiloxane – PDMS, 65μm polydimethylsiloxane/divinylbenzene – PDMS/DVB, 70μm carbowax/divinylbenzene – CW/DVB and 85μm carboxen/polydimethylsiloxane – CAR/PDMS) is presented for the characterization of the volatile metabolite profile of four selected Madeira isla ...
DOI:
10.1016/j.talanta.2010.10.064
PubMed:
21147335

38. Engineering GST M2-2 for High Activity with Indene 1,2-Oxide and Indication of an H-Site Residue Sustaining Catalytic Promiscuity

Author:
Malena A. Norrgård; Bengt Mannervik
Source:
Journal of molecular biology 2011 v.412 no.1 pp. 111-120
ISSN:
0022-2836
Subject:
benzyl isothiocyanate; catalytic activity; chemical reactions; glutathione transferase; humans; mutagenesis; mutants
Abstract:
... The substrate-binding H-site of human glutathione transferase (GST) M2-2 was subjected to iterative saturation mutagenesis in order to obtain an efficient enzyme with the novel epoxide substrate indene 1,2-oxide. Residues 10, 116, and 210 were targeted, and the activities with the alternative substrates, benzyl isothiocyanate and the prodrug azathioprine, undergoing divergent chemical reactions we ...
DOI:
10.1016/j.jmb.2011.07.039
PubMed:
21821040

39. Inhibition of Lipopolysaccharide-Induced Cyclooxygenase-2 and Inducible Nitric Oxide Synthase Expression by 4-[(2′-O-acetyl-α-L-Rhamnosyloxy)Benzyl]Isothiocyanate from Moringa oleifera

Author:
Eun-Jung Park; Sarot Cheenpracha; Leng Chee Chang; Tamara P. Kondratyuk; John M. Pezzuto
Source:
Nutrition and cancer 2011 v.63 no.6 pp. 971-982
ISSN:
1532-7914
Subject:
Moringa oleifera; benzyl isothiocyanate; chemoprevention; glycosides; inhibitory concentration 50; macrophages; medicinal properties; messenger RNA; mice; mitogen-activated protein kinase; nitric oxide; nitric oxide synthase; phosphorylation; prostaglandin synthase; proteins; signal transduction
Abstract:
... Moringa oleifera Lamarck is commonly consumed for nutritional or medicinal properties. We recently reported the isolation and structure elucidation of novel bioactive phenolic glycosides, including 4-[(2′-O-acetyl-α-L-rhamnosyloxy)benzyl]isothiocyanate (RBITC), which was found to suppress inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production in lipopolysaccharide-stimu ...
DOI:
10.1080/01635581.2011.589960
PubMed:
21774591
PubMed Central:
PMC3381517

40. Mitogen-activated protein kinase mediates the apoptosis of highly metastatic human non-small cell lung cancer cells induced by isothiocyanates

Author:
Huiqin Yan; Yu Zhu; Boning Liu; Heng Wu; Yongwen Li; Xiang Wu; Qinghua Zhou; Ke Xu
Source:
British journal of nutrition 2011 v.106 no.12 pp. 1779-1791
ISSN:
1475-2662
Subject:
apoptosis; benzyl isothiocyanate; caspase-3; cell cycle; cell growth; cysteine; genes; humans; lung neoplasms; metastasis; microarray technology; mitogen-activated protein kinase; oxidative stress; therapeutics; transcriptional activation
Abstract:
... Dietary isothiocyanates have been shown to possess anti-tumour activity, inhibiting several types of cultured human cancer cell growth. However, there are limited studies on their effects on cancer cell metastasis. Our previous study showed that benzyl isothiocyanate (BITC) and phenethyl isothiocyanate (PEITC) suppressed human lung cancer cell metastasis potential. In the present study, we found B ...
DOI:
10.1017/S0007114511002315
PubMed:
21733335

41. Nanoemulsions of Cancer Chemopreventive Agent Benzyl Isothiocyanate Display Enhanced Solubility, Dissolution, and Permeability

Author:
Qhattal, Hussaini Syed Sha; Wang, Shu; Salihima, Tri; Srivastava, Sanjay K.; Liu, Xinli
Source:
Journal of agricultural and food chemistry 2011 v.59 no.23 pp. 12396-12404
ISSN:
0021-8561
Subject:
absorption; benzyl isothiocyanate; bioavailability; chemoprevention; humans; nanoemulsions; permeability; solubility; vegetables
Abstract:
... Benzyl isothiocyanate (BITC), a compound found in cruciferous vegetables, is an effective chemopreventive agent. The objective of this study was to develop nanoemulsion formulations for the oral delivery of BITC. Optimized oil-in-water BITC nanoemulsions were prepared by a spontaneous self-nanoemulsification method and a homogenization-sonication method. Both nanoemulsions entrapped high amounts o ...
DOI:
10.1021/jf202612b
PubMed:
22011260

42. Nutritional composition of Rainbow papaya, the first commercialized transgenic fruit crop

Author:
Savarni Tripathi; Jon Y. Suzuki; James B. Carr; Grant T. McQuate; Stephen A. Ferreira; Richard M. Manshardt; Karen Y. Pitz; Marisa M. Wall; Dennis Gonsalves
Source:
Subtropical plant science 2011 v.24 no.2 pp. 140-147
ISSN:
0485-2044
Subject:
Carica papaya; Papaya ringspot virus; allergens; benzyl isothiocyanate; calcium; color; commercialization; cultivars; food safety; fruit crops; fruits; nutrient content; nutrients; nutrition; papain; pedigree; proteins; ripening; vitamin A; Hawaii
Abstract:
... Rainbow papaya (Carica papaya L.) is a genetically engineered (GE) cultivar with resistance to papaya ringspot virus (PRSV). This cultivar currently accounts for about 70% of Hawaii's papaya acreage. The nutritional composition of Rainbow papaya and a non-transgenic control were analyzed to address GE food safety concerns regarding the potential for altered nutritional composition and altered expr ...
DOI:
10.1016/j.jfca.2010.07.003

43. Phenethyl isothiocyanate sensitizes human cervical cancer cells to apoptosis induced by cisplatin

Author:
Xiantao Wang; Sudha Govind; Shyama P. Sajankila; Lixin Mi; Rabindra Roy; Fung‐Lung Chung
Source:
Molecular nutrition & food research 2011 v.55 no.10 pp. 1572-1581
ISSN:
1613-4125
Subject:
apoptosis; benzyl isothiocyanate; breast neoplasms; cell growth; cisplatin; cytotoxicity; epithelial cells; humans; mitogen-activated protein kinase; synergism; uterine cervical neoplasms; watercress
Abstract:
... Scope: Naturally‐occurring chemopreventive agent phenethyl isothiocyanate (PEITC), derived primarily from watercress, has been shown to inhibit cell growth and induce apoptosis in cancer cells. In this study, we examined the potential of PEITC in enhancing cisplatin‐induced apoptosis in cervical cancer cells and its mechanisms. Methods and results: HeLa cells were exposed to PEITC, cisplatin or bo ...
DOI:
10.1002/mnfr.201000560
PubMed:
21595016
PubMed Central:
PMC3561716

44. Stimulation of phagocytosis by sulforaphane

Author:
Hiroyuki Suganuma; Jed W. Fahey; Kelley E. Bryan; Zachary R. Healy; Paul Talalay
Source:
Biochemical and biophysical research communications 2011 v.405 no.1 pp. 146-151
ISSN:
0006-291X
Subject:
anti-inflammatory activity; benzyl isothiocyanate; fetal bovine serum; genes; inflammation; macrophage migration inhibitory factors; macrophages; mice; oxidative stress; phagocytosis; polystyrenes; toxicity; transcription factors; vegetables
Abstract:
... Sulforaphane, a major isothiocyanate derived from cruciferous vegetables, protects living systems against electrophile toxicity, oxidative stress, inflammation, and radiation. A major protective mechanism is the induction of a network of endogenous cytoprotective (phase 2) genes that are regulated by transcription factor Nrf2. To obtain a more detailed understanding of the anti-inflammatory and im ...
DOI:
10.1016/j.bbrc.2011.01.025
PubMed:
21219867
PubMed Central:
PMC3057406

45. A bioinspired look at the glucosinolate metabolic pathway. Structural insights into the reaction of benzyl isothiocyanate and d-glucosamine

Author:
Guadalupe Silvero; Martín Ávalos; Reyes Babiano; Pedro Cintas; José L. Jiménez; Juan C. Palacios
Source:
Tetrahedron 2011 v.67 no.40 pp. 7811-7820
ISSN:
0040-4020
Subject:
anticarcinogenic activity; benzyl isothiocyanate; biochemical pathways; chemical reactions; chemical structure; diet; foods; glucose; glucosinolates; inflammation; metabolites; oral administration; osteoarthritis
Abstract:
... Through a well-established enzymatic transformation glucosinolates release reactive isothiocyanates that can undergo further metabolic pathways affording a plethora of reactive metabolites. This study explores in detail the reaction of benzyl isothiocyanate, which possesses antitumor activity as alkylating agent, with d-glucosamine, commonly employed in oral treatments against osteoarthritis and i ...
DOI:
10.1016/j.tet.2011.07.073

46. Antimicrobial volatile glucosinolate autolysis products from Hornungia petraea (L.) Rchb. (Brassicaceae)

Author:
Niko S. Radulović; Milan S. Dekić; Zorica Z. Stojanović-Radić
Source:
Phytochemistry letters 2012 v.5 no.2 pp. 351-357
ISSN:
1874-3900
Subject:
Aspergillus fumigatus; Brassicaceae; Candida albicans; Micrococcus luteus; antibacterial properties; antifungal properties; autolysis; benzyl isothiocyanate; gas chromatography-mass spectrometry; glucosinolates; minimum inhibitory concentration; mustard oil; plant tissues; secondary metabolites
Abstract:
... Plant samples of Hornungia petraea were analyzed for glucosinolate (GLS) autolysis metabolites for the first time. GC–MS analysis of the autolysate and the synthesis of a series (12 compounds) of possible glucosinolate breakdown products revealed/corroborated the presence of glucoaubrietin, glucolimnanthin, glucolepigramin and glucotropaeolin in this species as the most likely “mustard oil” precur ...
DOI:
10.1016/j.phytol.2012.02.017

47. Benzyl Isothiocyanate (BITC) Induces G2/M Phase Arrest and Apoptosis in Human Melanoma A375.S2 Cells through Reactive Oxygen Species (ROS) and both Mitochondria-Dependent and Death Receptor-Mediated Multiple Signaling Pathways

Author:
Huang Su-Hua; Wu Liu-Wei; Huang An-Cheng; Yu Chien-Chih; Lien Jin-Cherng; Huang Yi-Ping; Yang Jai-Sing; Yang Jen-Hung; Hsiao Yu-Ping; Wood W. Gibson; Yu Chun-Shu; Chung Jing-Gung
Source:
Journal of agricultural and food chemistry 2012 v.60 no.2 pp. 665-675
ISSN:
1520-5118
Subject:
Western blotting; apoptosis; benzyl isothiocyanate; caspase-3; cell cycle; cell growth; flow cytometry; humans; melanoma; membrane potential; propidium; reactive oxygen species; signal transduction
Abstract:
... Benzyl isothiocyanates (BITC), a member of the isothiocyanate (ITC) family, inhibits cell growth and induces apoptosis in many types of human cancer cell lines. The present study investigated mechanisms underlying BITC-induced apoptosis in A375.S2 human melanoma cancer cells. To observe cell morphological changes and viability, flow cytometric assays, cell counting, and a contrast-phase microscopi ...
DOI:
10.1021/jf204193v
PubMed:
22148415

48. Contact and fumigant toxicity of Armoracia rusticana essential oil, allyl isothiocyanate and related compounds to Dermatophagoides farinae

Author:
Yeon‐Kyeong Yun; Hyun‐Kyung Kim; Jun‐Ran Kim; Kumnara Hwang; Young‐Joon Ahn
Source:
Pest management science 2012 v.68 no.5 pp. 788-794
ISSN:
1526-498X
Subject:
Armoracia rusticana; Dermatophagoides farinae; Glycyphagus domesticus; allyl isothiocyanate; benzyl benzoate; benzyl isothiocyanate; bioassays; chemical structure; containers; dibutyl phthalate; dust control; essential oils; fumigants; horseradish; lethal concentration 50; mortality; oils; toxicity; vapors
Abstract:
... BACKGROUND: The toxicity to adult Dermatophagoides farinae of allyl isothiocyanate identified in horseradish, Armoracia rusticana, oil and another 27 organic isothiocyanates was evaluated using contact + fumigant and vapour‐phase mortality bioassays. Results were compared with those of two conventional acaricides, benzyl benzoate and dibutyl phthalate. RESULTS: Horseradish oil (24 h LC50, 1.54 µg ...
DOI:
10.1002/ps.2327
PubMed:
22102526

49. Cytotoxicity of Benzyl Isothiocyanate in Normal Renal Proximal Tubular Cells and Its Modulation by Glutathione

Author:
Abe Naomi; Okuhira Masashi; Tsutsui Chiharu; Murata Yoshiyuki; Nakamura Yoshimasa
Source:
Journal of agricultural and food chemistry 2012 v.60 no.7 pp. 1887-1892
ISSN:
1520-5118
Subject:
benzyl isothiocyanate; cytotoxicity; glutathione; inhibitory concentration 50; necrosis; oxidative stress; swine
Abstract:
... In the present study, we examined the toxicity of benzyl ITC (BITC) and its urinary mercapturic acid metabolite (BITC-NAC), using a normal renal proximal tubular cell line, pig LLC-PK1. BITC increased cell death with an IC₅₀ value of about 7 μM, whereas the cytotoxic effect of BITC-NAC was five times weaker than that of BITC. We observed a significant necrosis of the compounds on LLC-PK1 cells wit ...
DOI:
10.1021/jf2052042
PubMed:
22296293

50. Determination of sinigrin, sinalbin, allyl- and benzyl isothiocyanates by RP-HPLC in mustard powder extracts

Author:
Saqer Herzallah; Richard Holley
Source:
Lebensmittel-Wissenschaft + Technologie 2012 v.47 no.2 pp. 293-299
ISSN:
0023-6438
Subject:
boiling; thermal stability; detection limit; freeze drying; sulfates; allyl isothiocyanate; benzyl isothiocyanate; solvents; sinigrin; hydrogen; hydrolysis; reversed-phase high performance liquid chromatography; powders; thioglucosidase; wavelengths; autoclaving
Abstract:
... A reversed phase-high performance liquid chromatography method was developed to quantify sinigrin, sinalbin, allyl isothiocyanate and benzyl isothiocyanate present in aqueous and freeze-dried yellow and Oriental (brown) mustard extract samples using two pre-treatment methods (autoclaving, boiling) to prevent degradation by myrosinase. The lowest detection limits for sinigrin and sinalbin were 0.05 ...
DOI:
10.1016/j.lwt.2012.01.022