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- Ganguli, Nirmalya, et al. Show all 6 Authors
- Applied microbiology and biotechnology 2018 v.102 no.14 pp. 6221-6234
- beta-casein; biopharmaceuticals; bioreactors; buffaloes; cost effectiveness; epithelial cells; gene expression; genetically modified organisms; germ cells; humans; interferon-gamma; lactation; mammary glands; membrane fusion; milk; nucleic acids; protein synthesis; risk; therapeutics; transgenes; transgenesis
- ... Protein expression in the milk of transgenic farmed animals offers a cost-effective system for producing therapeutics. However, transgenesis in farmed animals is not only cumbersome but also involves risk of potential hazard by germline gene integration, due to interruptions caused by the transgene in the native genome. Avoiding germline gene integration, we have delivered buffalo β-casein promote ...
- Ganguli, Nirmalya, et al. Show all 4 Authors
- Journal of biotechnology 2015 v.198 pp. 53-59
- buffaloes; recombinant DNA; epithelial cells; biopharmaceuticals; milk; epithelium; promoter regions; genetically modified organisms; bioreactors; gene expression; remediation; buffalo milk; mammary glands; patients; introns; females; humans; transcription (genetics); mice; transfection; exons; green fluorescent protein; beta-casein; lactation; microorganisms; body fluids; milk production; cost effectiveness
- ... Therapeutic proteins are produced in microbes, mammalian cell lines, and body fluids by applying recombinant DNA technology. They are required for compensating the deficiency of essential proteins in patients. Animal bioreactors producing such valuable bio-pharmaceuticals in body fluids have lately emerged as efficient and cost-effective expression systems. Promoters, along with other regulatory e ...