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You searched for: Subject term beta-glucosidase Remove constraint Subject term: beta-glucosidase Subject term active sites Remove constraint Subject term: active sites Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
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1. A Novel Three Domains Glycoside Hydrolase Family 3 from Sclerotinia sclerotiorum Exhibits β-Glucosidase and Exoglucanase Activities: Molecular, Biochemical, and Transglycosylation Potential Analysis

Author:
Haifa Chahed; Aymen Ezzine; Mohamed Amine Ben Mlouka; Christophe Rihouey; Julie Hardouin; Thierry Jouenne; M. Nejib Marzouki
Source:
Molecular biotechnology 2015 v.57 no.11-12 pp. 993-1002
ISSN:
1073-6085
Subject:
Sclerotinia sclerotiorum; active sites; amino acids; beta-glucosidase; crystal structure; fibronectins; fungi; gel chromatography; glucooligosaccharides; mass spectrometry; molecular models; molecular weight; pH; polyacrylamide gel electrophoresis; polypeptides
Abstract:
... The filamentous fungus Sclerotinia sclerotiorum produces a complete set of cellulolytic enzymes. We report here the purification and the biochemical characterization of a new β-glucosidase from S. sclerotiorum which belongs to the family 3 of glycoside hydrolases and that was named as SsBgl3. After two size-exclusion chromatography steps, purified protein bands of 80 and 90 kDa from SDS-PAGE were ...
DOI:
10.1007/s12033-015-9892-z

2. A cold-active β-glucosidase (Bgl1C) from a sea bacteria Exiguobacterium oxidotolerans A011

Author:
Chen, Shuilian; Hong, Yuzhi; Shao, Zongze; Liu, Ziduo
Source:
World journal of microbiology & biotechnology 2010 v.26 no.8 pp. 1427-1435
ISSN:
0959-3993
Subject:
EDTA (chelating agent); Exiguobacterium; active sites; alanine; amino acid sequences; bacteria; beta-glucosidase; buffers; calcium; cobalt; copper; genes; genomic libraries; industrial applications; mercury; molecular weight; nucleotides; open reading frames; pH; serine; sodium; temperature; valine
Abstract:
... By constructing a genomic library, a new gene encoding β-glucosidase (Bgl1C) was cloned from Exiguobacterium oxidotolerans A011, which was isolated from deep sea mud. The putative β-glucosidase gene consisted of an open reading frame (ORF) of 1,347 nucleotides, and encoded a protein of 448 amino acids with a predicted molecular weight of 51.6 kDa. Bgl1C belonged to the glycoside hydrolase family 1 ...
DOI:
10.1007/s11274-010-0317-7

3. A new β-glucosidase gene from the zygomycete fungus Rhizomucor miehei

Author:
Takó, Miklós; Tóth, Adél; Nagy, László G.; Krisch, Judit; Vágvölgyi, Csaba; Papp, Tamás
Source:
Antonie van Leeuwenhoek 2010 v.97 no.1 pp. 1-10
ISSN:
0003-6072
Subject:
Mucor; proteins; Rhizomucor miehei; plasmids; amino acids; active sites; introns; sequence homology; beta-glucosidase; fungi
Abstract:
... In this study, a β-glucosidase coding gene (bgl) of the zygomycete fungus Rhizomucor miehei has been cloned and characterized. The gene comprises a total of 2,826 bp including the coding sequence of a 717 amino acids length putative protein and 10 introns dispersed in the whole coding region. The putative N-and C-terminal catalytic domains (aa 68 to aa 274 and aa 358-601, respectively) were identi ...
DOI:
10.1007/s10482-009-9382-z

4. A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil)

Author:
Danyelle Toyama; Mariana Abrahão Bueno de Morais; Felipe Cardoso Ramos; Letícia Maria Zanphorlin; Celisa Caldana Costa Tonoli; Augusto Furio Balula; Fernando Pellon de Miranda; Vitor Medeiros Almeida; Sandro Roberto Marana; Roberto Ruller; Mario Tyago Murakami; Flavio Henrique-Silva
Source:
Biochimica et biophysica acta 2018 v.1866 pp. 569-579
ISSN:
1570-9639
Subject:
Escherichia coli; X-radiation; X-ray diffraction; active sites; beta-glucosidase; cellobiose; genes; glucose; glycosides; industrial applications; lakes; metagenomics; microorganisms; pH; surveys; Amazonia; Brazil
Abstract:
... The Amazon region holds most of the biological richness of Brazil. Despite their ecological and biotechnological importance, studies related to microorganisms from this region are limited. Metagenomics leads to exciting discoveries, mainly regarding non-cultivable microorganisms. Herein, we report the discovery of a novel β-glucosidase (glycoside hydrolase family 1) gene from a metagenome from Lak ...
DOI:
10.1016/j.bbapap.2018.02.001

5. Alkali-tolerant β-glucosidase produced by newly isolated Aspergillus fumigatus WL002 from rotten wood

Author:
Pinghua Cao; Lei Wang; Yaoyue Wang; Ning Zhou; Yulin Chen
Source:
International Biodeterioration & Biodegradation 2015 v.105 pp. 276-282
ISSN:
0964-8305
Subject:
lignocellulose; polyacrylamide gel electrophoresis; nucleotides; Aspergillus fumigatus; genes; polypeptides; open reading frames; internal transcribed spacers; amino acids; active sites; molecular weight; metal ions; beta-glucosidase; carboxymethylcellulose; Escherichia coli; polymerase chain reaction; biotransformation; temperature; wood; pH; biodegradation; fungi
Abstract:
... A lignocellulosic decomposing fungus WL002 with a high β-glucosidase activity was isolated from rotten wood. The fungus was classified and designated as Aspergillus fumigatus WL002 through morphological and internal transcribed spacer sequence (ITS) analysis. A putative β-glucosidase gene, bglW, which was cloned from the isolated strain by PCR amplification, consists of an open reading frame of 25 ...
DOI:
10.1016/j.ibiod.2015.09.010

6. An acid-stable β-glucosidase from Aspergillus aculeatus: Gene expression, biochemical characterization and molecular dynamics simulation

Author:
Yu Li; Xiaoyan Hu; Jingcheng Sang; Ying Zhang; Huitu Zhang; Fuping Lu; Fufeng Liu
Source:
International journal of biological macromolecules 2018 v.119 pp. 462-469
ISSN:
0141-8130
Subject:
Aspergillus aculeatus; active sites; barium; beta-glucosidase; beverages; biofuels; calcium; catalytic activity; cellobiose; copper; denaturation; drugs; enzyme activity; gene expression; hydrogen bonding; iron; magnesium; manganese; molecular dynamics; pH; solvents; surface area; temperature; zinc
Abstract:
... β-Glucosidases hydrolyze terminal, non-reducing β-d-glucosyl residues and thereby release β-d-glucose. They have applications in the production of biofuels, beverages and pharmaceuticals. In this study, a β-glucosidase derived from Aspergillus aculeatus (BGLA) was expressed, characterized, and the molecular mechanism of its acid denaturation was comprehensively probed. BGLA exhibited maximal activ ...
DOI:
10.1016/j.ijbiomac.2018.07.165

7. BadGluc, a β-glucosidase from Bjerkandera adusta with anthocyanase properties

Author:
Christoph J. Behrens; Nina K. Krahe; Diana Linke; Ralf G. Berger
Source:
Bioprocess and biosystems engineering 2018 v.41 no.9 pp. 1391-1401
ISSN:
1615-7591
Subject:
temperature; proteins; aqueous solutions; Komagataella pastoris; Bjerkandera adusta; small fruits; decolorization; myrtillin; food industry; amino acid sequences; genes; active sites; fabrics; hydrolysis; molecular cloning; pH; beta-glucosidase; detergents
Abstract:
... A glycosidase of the basidiomycete Bjerkandera adusta (BadGluc) was found in screenings to possess a strong decolorizing ability towards malvidin-3-galactoside, an anthocyanin abundant in various berry fruits. The BadGluc was purified from the culture supernatant via FPLC, and the corresponding gene was identified which showed low similarity to other characterized glucosidases. Scanning the primar ...
DOI:
10.1007/s00449-018-1966-4

8. Biomimetic Cascade Network between Interactive Multicompartments Organized by Enzyme-Loaded Silica Nanoreactors

Author:
Seong-Min Jo; Frederik R. Wurm; Katharina Landfester
Source:
ACS applied materials & interfaces 2018 v.10 no.40 pp. 34230-34237
ISSN:
1944-8252
Subject:
active sites; beta-glucosidase; biomimetics; emulsions; encapsulation; glucose oxidase; heat; models; peroxidase; proteolysis; silica
Abstract:
... Physical separation of reactions by interactive multicompartments in biological cells is an attractive motif to design efficient microreactors that create biomimetic cascade reactions. We present an aqueous compartment with three different subcompartments that comprise of silica nanoreactors with encapsulated enzymes, namely, β-glucosidase, glucose oxidase, and peroxidase, providing a model cascad ...
DOI:
10.1021/acsami.8b11198

9. Cell wall structure suitable for surface display of proteins in Saccharomyces cerevisiae

Author:
Hiroyuki Matsuoka; Kazuya Hashimoto; Aki Saijo; Yuki Takada; Akihiko Kondo; Mitsuyoshi Ueda; Hiroshi Ooshima; Taro Tachibana; Masayuki Azuma
Source:
Yeast 2014 v.31 no.2 pp. 67-76
ISSN:
0749-503X
Subject:
green fluorescent protein; cell walls; enzyme activity; Saccharomyces cerevisiae; microorganisms; active sites; mutants; endo-1,4-beta-glucanase; beta-glucosidase; carboxymethylcellulose
Abstract:
... A display system for adding new protein functions to the cell surfaces of microorganisms has been developed, and applications of the system to various fields have been proposed. With the aim of constructing a cell surface environment suitable for protein display in Saccharomyces cerevisiae, the cell surface structures of cell wall mutants were investigated. Four cell wall mutant strains were selec ...
DOI:
10.1002/yea.2995
PubMed:
24357429

10. Cellulase gene expression profiles in termites according to habitat and diet

Author:
Kyungjae Andrew Yoon; Ju Hyeon Kim; Chae Eun Hwang; Young Ho Kim; Won Hoon Lee; Si Hyeock Lee
Source:
Journal of Asia-Pacific entomology 2015 v.18 no.3 pp. 369-375
ISSN:
1226-8615
Subject:
diet; Macrotermes; wood; cellulose 1,4-beta-cellobiosidase; enzyme activity; cattle manure; expressed sequence tags; phylogeny; cellulose; gene expression; endo-1,4-beta-glucanase; Microtermes; active sites; cellulolytic microorganisms; tissues; RNA; suppression subtractive hybridization; habitats; beta-glucosidase
Abstract:
... To investigate the expression profiles of cellulase genes in termites due to differences in habitat and diet, we collected one species (Microtermes pakistanicus) from dry wood, one species (Macrotermes gilvus) from rotten damp wood and one species (Microtermes sp.) from cow dung. Total RNA was extracted from the hind guts containing paunch and from other tissues from each collected species and use ...
DOI:
10.1016/j.aspen.2015.04.007

11. Characterization of a novel manganese dependent endoglucanase belongs in GH family 5 from Phanerochaete chrysosporium

Author:
Nguyen Duc Huy; Cu Le Nguyen; Han-Sung Park; Nguyen Hoang Loc; Myoung-Suk Choi; Dae-Hyuk Kim; Jeong-Woo Seo; Seung-Moon Park
Source:
Journal of bioscience and bioengineering 2016 v.121 no.2 pp. 154-159
ISSN:
1389-1723
Subject:
manganese; thermal stability; Komagataella pastoris; polyacrylamide gel electrophoresis; cellulose 1,4-beta-cellobiosidase; glucose; amino acids; active sites; carbohydrate binding; molecular weight; beta-glucosidase; saccharification; complementary DNA; temperature; enzyme activity; biomass; Phanerochaete chrysosporium; glycosides; barley straw; pH; endo-1,4-beta-glucanase
Abstract:
... The cDNA encoding a putative glycoside hydrolase family 5, which has been predicted to be an endoglucanase (PcEg5A), was cloned from Phanerochaete chrysosporium and expressed in Pichia pastoris. PcEg5A contains a carbohydrate-binding domain and two important amino acids, E209 and E319, playing as proton donor and nucleophile in substrate catalytic domain. SDS-PAGE analysis indicated that the recom ...
DOI:
10.1016/j.jbiosc.2015.06.009

12. Characterization of a novel thermostable β-glucosidase from a metagenomic library of termite gut

Author:
Qianfu Wang; Changli Qian; Xiao-Zhou Zhang; Nin Liu; Xing Yan; Zhihua Zhou
Source:
Enzyme and microbial technology 2012 v.51 no.6-7 pp. 319-324
ISSN:
0141-0229
Subject:
Acidothermus cellulolyticus; Escherichia coli; Isoptera; Trichoderma reesei; active sites; amino acids; beta-glucosidase; cellulose; cellulose 1,4-beta-cellobiosidase; digestive system; endo-1,4-beta-glucanase; fungi; genes; genomic libraries; glycosides; half life; industrial applications; molecular weight; pH; polyacrylamide gel electrophoresis; polypeptides; synergism; temperature; thermal stability
Abstract:
... A novel β-glucosidase-encoding gene, bgl-gs1, which was identified from a positive fosmid clone in a metagenomic library of the gut of Globitermes sulphureus, encodes a 455 amino acid polypeptide that contains a catalytic domain belonging to glycoside hydrolase family 1 (GH1). It was expressed in Escherichia coli BL21 (DE3) and the expression product showed a molecular mass of ∼51.7kDa by SDS-PAGE ...
DOI:
10.1016/j.enzmictec.2012.07.015

13. Characterization of the cellulase-secretome produced by the Antarctic bacterium Flavobacterium sp. AUG42

Author:
Lorena M. Herrera; Victoria Braña; Laura Franco Fraguas; Susana Castro-Sowinski
Source:
Microbiological research 2019 v.223-225 pp. 13-21
ISSN:
0944-5013
Subject:
Flavobacterium; Oligochaeta; active sites; bacteria; bacterial growth; beta-glucosidase; biorefining; carbohydrate binding; carbon; carboxymethylcellulose; endo-1,4-beta-glucanase; enzyme activity; glucose; glycosides; industry; mass spectrometry; pH; protein secretion; reducing sugars; saccharification; temperature; Antarctic region
Abstract:
... Flavobacterium sp. AUG42 is a cellulase-producing bacterium isolated from the Antarctic oligochaete Grania sp. (Annelida). In this work, we report that AUG42 produces a glycoside hydrolase cocktail with CMCase, PASCase and cellobiase activities (optimum pHs and temperatures ranging from 5.5 to 6.5 and 40 to 50 °C, respectively). The time-course analyses of the bacterial growth and cellulase produc ...
DOI:
10.1016/j.micres.2019.03.009

14. Cloning and expression of β-glucosidase gene from Bacillus licheniformis into E. coli BL 21 (DE3)

Author:
Sana Zahoor; Muhammad Javed; Muhammad Aftab; Ikram-ul-Haq
Source:
Biologia 2011 v.66 no.2 pp. 213-220
ISSN:
1336-9563
Subject:
Bacillus licheniformis; Escherichia coli; activation energy; active sites; beta-glucosidase; endo-1,4-beta-glucanase; enthalpy; genes; molecular cloning; molecular weight; pH; polyacrylamide gel electrophoresis; saccharification; sodium dodecyl sulfate; wheat straw
Abstract:
... A 1.4 Kb fragment of Bacillus licheniformis ATCC 14580 encoding β-glucosidase was cloned and expressed in Escherichia coli. β-Glucosidase expressed by E. coli harboring cloned gene was located entirely in the intracellular fraction. Recombinant β-glucosidase protein was purified to homogeneity level and the molecular weight was found to be 53 kDa using sodium dodecyl sulfate polyacrylamide gel ele ...
DOI:
10.2478/s11756-011-0020-7

15. Combined SAXS and computational approaches for structure determination and binding characteristics of Chimera (CtGH1-L1-CtGH5-F194A) generated by assembling β-glucosidase (CtGH1) and a mutant endoglucanase (CtGH5-F194A) from Clostridium thermocellum

Author:
Priyanka Nath; Kedar Sharma; Krishan Kumar; Arun Goyal
Source:
International journal of biological macromolecules 2020 v.148 pp. 364-377
ISSN:
0141-8130
Subject:
Clostridium thermocellum; active sites; beta-glucosidase; binding properties; catalytic activity; endo-1,4-beta-glucanase; energy; hydrolysis; mutants; prediction
Abstract:
... Chimera (CtGH1-L1-CtGH5-F194A) developed by fusing β-glucosidase (CtGH1) at N-terminal and endoglucanase (CtGH5-F194A) at C-terminal was structurally characterized. Its secondary structure analysis by CD showed 38% α-helix, 9.3% β-sheets and 52.7% random coils corroborating with prediction. In-silico modeled structure of Chimera comprised two modules, CtGH1 and CtGH5-F194A displaying (α/β)₈ fold. ...
DOI:
10.1016/j.ijbiomac.2020.01.116

16. Complexation of alkyl glycosides with α-cyclodextrin can have drastically different effects on their conversion by glycoside hydrolases

Author:
Mohd Younis Rather; Eva Nordberg Karlsson; Patrick Adlercreutz
Source:
Journal of biotechnology 2015 v.200 pp. 52-58
ISSN:
0168-1656
Subject:
Aspergillus niger; Thermotoga neapolitana; active sites; almonds; alpha-cyclodextrin; beta-glucosidase; binding sites; carbohydrate binding; glucose; glycosides; hydrolysis; micelles
Abstract:
... Substrates present in aggregated forms, such as micelles, are often poorly converted by enzymes. Alkyl glycosides constitute typical examples and the critical micelle concentration (CMC) decreases with increasing length of the alkyl group. In this study, possibilities to hydrolyse alkyl glycosides by glycoside hydrolases were explored, and α-cyclodextrin was used as an agent to form inclusion comp ...
DOI:
10.1016/j.jbiotec.2015.02.019

17. Cooperation of enzymes involved in carbohydrate digestion of Colorado potato beetle (Leptinotarsa decemlineata, Say)

Author:
E. Szilágyi; C. Hámori; P. Bíró-Molnár; L. Kandra; J. Remenyik; G. Gyémánt
Source:
Bulletin of entomological research 2019 v.109 no.5 pp. 695-700
ISSN:
1475-2670
Subject:
Leptinotarsa decemlineata; Solanum tuberosum; active sites; alpha-amylase; beta-glucosidase; binding sites; carbohydrates; catalytic activity; digestion; enzyme activity; enzyme substrates; high performance liquid chromatography; hydrolysis; insect larvae; nutrients; pH; pests; plant protection; potatoes; swine; temperature
Abstract:
... Colorado potato beetle (Leptinotarsa decemlineata, Say) is the main pest of Solanaceae and its survival is mainly dependent on the carbohydrate digestion. Characterizing the gut enzymes may help us with finding effective inhibitors for plant protection. Activity measurements revealed that gut extracts contain α- and β-glucosidase in addition to α-amylase. For larvae, amylase activity was detected ...
DOI:
10.1017/S0007485319000099

18. Elucidating the regulation of glucose tolerance in a β-glucosidase from Halothermothrix orenii by active site pocket engineering and computational analysis

Author:
Sushant K. Sinha; Shibashis Das; Sukanya Konar; Pradip Kr. Ghorai; Rahul Das; Supratim Datta
Source:
International journal of biological macromolecules 2020 v.156 pp. 621-632
ISSN:
0141-8130
Subject:
Escherichia coli; Halothermothrix orenii; active sites; beta-glucosidase; catalytic activity; engineering; enzyme activity; enzyme inhibition; genes; glucose; glucose tolerance; hydrolysis; hydrophobicity; molecular dynamics; nuclear magnetic resonance spectroscopy; simulation models
Abstract:
... β-Glucosidase catalyzes the hydrolysis of β-1,4 linkage between two glucose molecules in cello-oligosaccharides and is prone to inhibition by the reaction product glucose. Relieving the glucose inhibition of β-glucosidase is a significant challenge. Towards the goal of understanding how glucose interacts with β-glucosidase, we expressed in Escherichia coli, the Hore_15280 gene encoding a β-glucosi ...
DOI:
10.1016/j.ijbiomac.2020.04.036

19. Endogenous cellulase production in the leaf litter foraging mangrove crab Parasesarma erythodactyla

Author:
T.H. Hanh Bui; Shing Yip Lee
Source:
Comparative Biochemistry and Physiology, Part B 2015 v.179 pp. 27-36
ISSN:
1096-4959
Subject:
DNA fragmentation; Uca; Varunidae; active sites; amino acids; arthropods; beta-glucosidase; cellulose; complementary DNA; crabs; detritivores; digestion; digestive juices; endo-1,4-beta-glucanase; exons; foraging; hepatopancreas; introns; molecular cloning; muscle tissues; open reading frames; pH; plant litter; polymerase chain reaction; sequence analysis; temperature
Abstract:
... The sesarmid crab Parasesarma erythodactyla consumes large amounts of mangrove leaf litter but its biochemical capacity for cellulose digestion is poorly known. We demonstrate the presence of endo-β-1,4-glucanase, β-glucosidase and total cellulase activities in the digestive juice of this crab. The highest total cellulase activity was observed at mildly acidic pH (5 to 6) and temperature between 3 ...
DOI:
10.1016/j.cbpb.2014.09.004

20. Engineering novel S-glycosidase activity into extremo-adapted β-glucosidase by rational design

Author:
Nouarh Almulhim; Nicholas R. Moody; Francesca Paradisi
Source:
Applied microbiology and biotechnology 2020 v.104 no.10 pp. 4407-4415
ISSN:
0175-7598
Subject:
Brevicoryne brassicae; Halothermothrix orenii; Thermus nonproteolyticus; active sites; arginine; beta-glucosidase; engineering; enzyme activity; glycosidic linkages; isothiocyanates; mutants; mutation; neoplasms; sulfur; thioglucosidase
Abstract:
... The breakdown of sulphur glycosidic bonds in thioglycosides can produce isothiocyanate, a chemoprotective agent linked to the prevention of cancers; however, only a handful of enzymes have been identified that are k0nown to catalyse this reaction. Structural studies of the myrosinase enzyme, which is capable of hydrolysing the thioglycosidic bond, have identified residues that may play important r ...
DOI:
10.1007/s00253-020-10582-3