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... (R)Mandelonitrile and the cyanogenic glucoside prunasin have been established as the source of HCN in all stages of the Australian beetle Paropsis atomaria. Quantitative data for both compounds and all life stages are presented. The larvae contain a β-glucosidase activity capable of hydrolysing prunasin and also β-cyanoalanine synthase activity enabling the disposal of free cyanide. ...
... The activities of seven hydrolytic enzymes (L-alanine aminopeptidase, esterase, α-and β-glucosidase, phosphomonoesterase, phosphodiesterase, sulfatase) were monitored during 1 year in parallel and serial treatment units of the biological stage of a communal wastewater treatment plant. The spatial homogeneity of enzyme activities was high (coefficients of variation <10 % for the entire treatment st ...
... Bgl1B (ACY09072) is a new GH1 family β-glucosidase derived from a marine microbial metagenomic library. In our previous study, the activity of recombinant Bgl1B was competitively inhibited by glucose with an IC₅₀ value of 30mM. Based on amino acids sequence alignment with other GH1 β-glucosidases, including BglB from Paenibacillus polymyxa whose structure has been solved, the 184th and 409th resid ...
... Production of renewable fuels and chemicals from cellulosic biomass is a critical step towards energy sustainability and reduced greenhouse gas emissions. Microbial cells have been engineered for producing chemicals from cellulosic sugars. Among these chemicals, 2,3-butanediol (2,3-BDO) is a compound of interest due to its diverse applications. While microbial production of 2,3-BDO with high yield ...
... We engineered efficient 2,3-butanediol (23BD) production from cellobiose using Bacillus subtilis. First, we found that B. subtilis harboring an empty vector could produce 23BD from cellobiose. However, productivity using cellobiose as a carbon source was lower than that when using glucose. This lower productivity was improved by adding purified beta-glucosidase from Thermobifida fusca YX (Tfu_0937 ...
Daniel Maag; Claudio Dalvit; Damien Thevenet; Angela Köhler; Felipe C. Wouters; Daniel G. Vassão; Jonathan Gershenzon; Jean-Luc Wolfender; Ted C.J. Turlings; Matthias Erb; Gaetan Glauser
... In order to defend themselves against arthropod herbivores, maize plants produce 1,4-benzoxazin-3-ones (BXs), which are stored as weakly active glucosides in the vacuole. Upon tissue disruption, BXs come into contact with β-glucosidases, resulting in the release of active aglycones and their breakdown products. While some aglycones can be reglucosylated by specialist herbivores, little is known ab ...
... Medicinal plant extracts of five plants; Adhatoda vasica, Eucalyptus globulus, Lantana camara, Nerium oleander and Ocimum basilicum collected from Cairo, Egypt were evaluated against Fusarium oxysporum f. sp. lycopersici race 3 in vitro conditions using water and certain organic solvents. The results revealed that cold distilled water extract ...
... β-Glucosidase activity assays constitute an important indicator for the early diagnosis of neonatal necrotizing enterocolitis and qualitative changes in medicinal plants. The drawbacks of the existing methods are high consumption of both time and reagents, complexity in operation, and requirement of expensive instruments and highly trained personnel. The present study provides a simplified, highly ...
... Desert locusts, Schistocerca gregaria (Forskal) (Orthoptera, Acrididae), are generalist herbivores that, in the Sahara desert, may at times feed only on Schouwia purpurea (Forskal) (Brassicaceae), that is 10 times richer in thioglucosides than currently observed in other crucifers (> 100 micromoles/g d.w.). Thioglucosides, when ingested, release products that are usually toxic to generalist insect ...
... The discovery of cytosolic ABA receptors is an important breakthrough in stomatal research; signaling via these receptors is involved in determining the basal stomatal conductance and stomatal responsiveness. However, the source of ABA in guard cells is still not fully understood. The level of ABA increases in guard cells by de novo synthesis, recycling from inactive conjugates via β-glucosidases ...
Erika Valente de Medeiros; Krystal de Alcantara Notaro; Jamilly Alves de Barros; Wendson da Silva Moraes; Aline Oliveira Silva; Keila Aparecida Moreira
... The transformation of tropical dry forests in intensive agricultural areas affects soil organic carbon with consequence in microbial and enzymatic activities, regardless of the type of use. The activities of soil enzymes (fluorescein diacetate hydrolysis dehydrogenase, β-glucosidase, arylsulfatase, urease, acid phosphatase and alkaline phosphatase), total organic carbon (TOC) and soil microbial bi ...
... We studied in the laboratory the bioremediation effects over a 100-day period of three edaphic biostimulants (BS) obtained from sewage sludge (SS) and from two different types of chicken feathers (CF1 and CF2), in a soil polluted with three polycyclic aromatic hydrocarbons (PAH) (phenanthrene, Phe; pyrene, Py; and benzo(a)pyrene, BaP), at a concentration of 100mgkg−1 soil. We determined their effe ...
... Light-induced aging of lignocellulosic materials has been studied with a new technique involving selectively α-, β-, and γ-(13)C-enriched cell wall-dehydrogenation polymers (CW-DHPs) and solid state (13)C NMR spectroscopy. The results from cross-polarization magic angle spinning (CP/MAS) (13)C NMR experiments of unirradiated and irradiated CW-DHP have revealed mainly a decrease in the amount of en ...
... The potential of cellulase enzymes in the developing and ongoing “biorefinery” industry has provided a great motivation to develop an efficient cellulase mixture. Recent work has shown how important the role that the so-called accessory enzymes can play in an effective enzymatic hydrolysis. In this study, three newest Novozymes Cellic CTec cellulase preparations (CTec 1/2/3) were compared to hydro ...
DNA fragmentation; Pectobacterium carotovorum subsp. carotovorum; beta-glucosidase; genes; glutamic acid; hydrolysis; magnesium; open reading frames; pH; polyacrylamide gel electrophoresis; promoter regions; proteins; sequence analysis; terminal repeat sequences
Abstract:
... Two cas genes were isolated from Pectobacterium carotovorum subsp. carotovorum LY34 (Pcc LY34). Sequence analysis of the 4873bp cloned DNA fragment (accession number AY866383) revealed two open reading frames (casF and casB) that are predicted to encode 658 and 467 amino acid proteins, respectively. The CasF protein is similar to other PTS enzyme II components. casB encodes β-glucosidase, a member ...
... The ER body is an endoplasmic reticulum (ER)-derived organelle. Because ER bodies are induced by wounding and methyl jasmonate (MeJA) treatment in rosette leaves, they might be responsible for defense systems. Recently, we isolated nai1 mutants that have no ER body and showed that the levels of PYK10 and PBP1 (PYK10-binding protein 1: At3g16420) were decreased in nai1 mutants. PYK10 is a {szligbet ...
... The β-d-glucosidases from wheat (Triticum aestivum) and rye (Secale cereale) hydrolyze benzoxazinone–glucose conjugates. Although wheat and rye glucosidases have high sequence identity, they have different substrate preferences; the wheat enzyme favors DIMBOA-Glc (2-O-β-d-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one) over DIBOA-Glc (7-demethoxy-DIMBOA-Glc), whereas the rye enzyme prefer ...
... Sucrose presence and concentration modulated in different ways and to different extents the activity of six plant glycoside hydrolases (PGHs) extracted from apple callus cultures, both in the water soluble fraction (WS-F) and in the NaCl-released fraction (NaCl-F). β-d-Glucosidase activity increased because of sucrose starvation and the addition of sucrose decreased both WS-F and NaCl-F β-d-glucos ...
... .The fig leaf roller or Fig-tree Skeletoniser, Choreutis nemorana (Lep.: Choreutidae), is a destructive pest of fig trees found in some fig-growing areas of Iran. The larvae feed on the upper level of leaves, near the main vein. In this study, digestive carbohydrases including α-glucosidase, β-glucosidase, α-galactosidase, β-galactosidase and proteinases including trypsin, chymotrypsin and elastas ...
... The level of activity of extracellular enzymes was determined on two transects characterised by different anthropic pressure on a sandy beach in Ustka, the southern coast of the Baltic Sea. Generally, the level of activity of the studied enzymes was higher on the transect characterised by high anthropic pressure. The ranking order of the mean enzyme activity rates in the sand was as follows: lipas ...