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You searched for: Subject term binding sites Remove constraint Subject term: binding sites Subject term immobilized enzymes Remove constraint Subject term: immobilized enzymes
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1. A comparative evaluation of the activity modulation of flavo and non-flavo enzymes induced by graphene oxide

Author:
Susmita Maiti; Somashree Kundu; Chandra Nath Roy; Debasmita Ghosh; Tushar Kanti Das; Abhijit Saha
Source:
Journal of materials chemistry B 2017 v.5 no.14 pp. 2601-2608
ISSN:
2050-7518
Subject:
binding sites; drugs; flavin-adenine dinucleotide; fluorescence; genes; glucose oxidase; graphene; graphene oxide; immobilized enzymes; industrial applications; lactate dehydrogenase; moieties; neoplasms; therapeutics; tryptophan; water solubility
Abstract:
... Graphene, and its water soluble derivative graphene oxide, has shown great promise in various biomedical applications, such as cancer therapeutics, drug delivery, etc. and in industrial applications such as enzyme immobilization, etc. Thus, modulation of the activities of different classes of enzymes by graphene materials is an important aspect in the formulation of different biological applicatio ...
DOI:
10.1039/c7tb00083a

2. A molecular chromatographic approach to study the effects of OH and NO on acetylcholinesterase activity

Author:
Firas Ibrahim; Claire André; Rania Aljhni; Tijani Gharbi; Yves Claude Guillaume
Source:
Journal of Molecular Catalysis. B, Enzymatic 2013 v.94 pp. 136-140
ISSN:
1381-1177
Subject:
Alzheimer disease; acetylcholine; acetylcholinesterase; active sites; binding sites; catalytic activity; chromatography; hydroxyl radicals; immobilized enzymes; nitric oxide; serine proteinases
Abstract:
... Acetylcholinesterase (AChE) is a serine protease that hydrolyzes the neurotransmitter acetylcholine. Here, the effects of hydroxyl radical (OH) and nitric oxide (NO) on AChE activity were studied using a biochromatographic process. The enzyme was immobilized on an ethylenediamine (EDA) monolithic convective interaction media (CIM) disk. The AChE enzymatic mechanism was demonstrated from the chroma ...
DOI:
10.1016/j.molcatb.2013.04.014

3. A simple approach to a vastly improved acetylcholinesterase activity and stability at elevated temperatures using magnetic microbeads and poly(N-(3-aminopropyl methacrylamide)) hydrogel supports

Author:
Lisa C. Shriver-Lake; Paul T. Charles; Andre A. Adams; Jake Fontana; Brett D. Martin
Source:
Journal of molecular catalysis 2016 v.134 pp. 61-69
ISSN:
1381-1177
Subject:
acetylcholinesterase; binding sites; catalytic activity; encapsulation; enzyme stability; hydrocolloids; immobilized enzymes; pH; polymers; primary amines; temperature; thermal stability
Abstract:
... The thermal stabilization of enzymes is a critical factor in the development and reliability of enzyme-based processes and functional materials. Using a simple amine coupling approach for enzyme immobilization onto magnetic microbeads, followed by encasement of the beads in a hydrogel, we demonstrate that the thermal stability of the enzyme acetylcholinesterase can be increased dramatically. For e ...
DOI:
10.1016/j.molcatb.2016.09.007

4. Amine-functionalized magnetic nanocomposite particles for efficient immobilization of lipase: effects of functional molecule size on properties of the immobilized lipase

Author:
Parvaneh Esmaeilnejad-Ahranjani; Mohammad Kazemeini; Gurvinder Singh; Ayyoob Arpanaei
Source:
RSC advances 2015 v.5 no.42 pp. 33313-33327
ISSN:
2046-2069
Subject:
Burkholderia cepacia; Fourier transform infrared spectroscopy; X-ray diffraction; binding sites; carboxylic ester hydrolases; catalytic activity; chemical bonding; circular dichroism spectroscopy; cost effectiveness; energy-dispersive X-ray analysis; immobilized enzymes; iron oxides; magnetism; models; molecular weight; nanocomposites; oxygen; polyethyleneimine; silica; storage quality; thermogravimetry; ultraviolet-visible spectroscopy; zeta potential
Abstract:
... A cost-effective design of reusable enzyme-functionalized particles with better catalytic activity is of great scientific interest due to their applications in a wide range of catalytic reactions in several industrial processes. In this work, a systematic approach for preparing amine-functionalized magnetic nanocomposite particles through the surface modification of core/shell type Fe₃O₄ cluster@S ...
DOI:
10.1039/c5ra02471d

5. Bio-Solid-Phase Extraction/Tandem Mass Spectrometry for Identification of Bioactive Compounds in Mixtures

Author:
Forsberg Erica M.; Brennan John D.
Source:
Analytical chemistry 2014 v.86 no.16 pp. 8457-8465
ISSN:
1520-6882
Subject:
acetic acid; adenosine deaminase; binding sites; bioactive compounds; bioassays; electrospray ionization mass spectrometry; enzyme inhibitors; immobilized enzymes; ionization; ligands; protein binding; rapid methods; silica; solid phase extraction; spectrometers; tandem mass spectrometry; washing
Abstract:
... We describe a two-step column-based bioassay method with tandem mass spectrometric detection for rapid identification of bioactive species in mixtures. The first step uses an immobilized enzyme reactor (IMER) column interfaced to an electrospray ionization mass spectrometer (ESI-MS) to identify mixtures containing bioactive compounds (i.e., enzyme inhibitors), while the second step uses bioselecti ...
DOI:
10.1021/ac5022166

6. Catalytic performance and molecular dynamic simulation of immobilized CC bond hydrolase based on carbon nanotube matrix

Author:
Hao Zhou; Yuanyuan Qu; Chunlei Kong; Duanxing Li; E. Shen; Qiao Ma; Xuwang Zhang; Jingwei Wang; Jiti Zhou
Source:
Colloids and Surfaces B: Biointerfaces 2014 v.116 pp. 365-371
ISSN:
0927-7765
Subject:
adsorption; binding sites; carbon nanotubes; catalytic activity; circular dichroism spectroscopy; colloids; fluorescence; hydrolases; immobilized enzymes; molecular dynamics
Abstract:
... Carbon nanotube (CNT) has been proved to be a kind of novel support for enzyme immobilization. In this study, we tried to find the relationship between conformation and catalytic performance of immobilized enzyme. Two CC bond hydrolases BphD and MfphA were immobilized on CNTs (SWCNT and MWCNT) via physical adsorption and covalent attachment. Among the conjugates, the immobilized BphD on chemically ...
DOI:
10.1016/j.colsurfb.2014.01.018

7. Computational, electrochemical, and spectroscopic, studies of acetycholinesterase covalently attached to carbon nanotubes

Author:
Murilo F. Cabral; Joseph D. Barrios; Erica M. Kataoka; Sergio A.S. Machado; Emanuel Carrilho; Carlos D. Garcia; Arturo A. Ayon
Source:
Colloids and Surfaces B: Biointerfaces 2013 v.103 pp. 624-629
ISSN:
0927-7765
Subject:
acetylcholinesterase; binding sites; carbon nanotubes; colloids; dielectric spectroscopy; electrochemistry; electrodes; electron transfer; energy; immobilized enzymes; infrared spectroscopy; physicochemical properties
Abstract:
... This manuscript describes results related to the characterization of electrodes modified with a composite of acetylcholinesterase covalently bound to carbon nanotubes (CNT). The characterization was performed by computational methods and complemented by cyclic voltammetry, infrared spectroscopy, and electrochemical impedance spectroscopy. In-silico simulations enabled the identification of the bin ...
DOI:
10.1016/j.colsurfb.2012.08.039

8. Electrochemical sensing platform amplified with a nanobiocomposite of L-phenylalanine ammonia-lyase enzyme for the detection of capsaicin

Author:
Myalowenkosi I. Sabela; Thabani Mpanza; Suvardhan Kanchi; Deepali Sharma; Krishna Bisetty
Source:
Biosensors & bioelectronics 2016 v.83 pp. 45-53
ISSN:
0956-5663
Subject:
adsorption; binding sites; biosensors; capsaicin; carbon nanotubes; detection limit; electrochemistry; electrodes; electron transfer; fruits; hydrogen bonding; hydrophobicity; immobilized enzymes; infrared spectroscopy; phenylalanine; phenylalanine ammonia-lyase; platinum; reflectance; thermogravimetry; transmission electron microscopy
Abstract:
... The present study involves the development of a sensitive electrochemical biosensor for the determination of capsaicin extracted from chilli fruits, based on a novel signal amplification strategy using enzyme technology. For the first time, platinum electrode modified with multiwalled carbon nanotubes where phenylalanine ammonia-lyase enzyme was immobilized using nafion was characterized by attenu ...
DOI:
10.1016/j.bios.2016.04.037

9. Expression, One-Step Purification, and Immobilization of HaloTagTM Fusion Proteins on Chloroalkane-Functionalized Magnetic Beads

Author:
Motejadded, Hassan; Kranz, Bertolt; Berensmeier, Sonja; Franzreb, Matthias; Altenbuchner, Josef
Source:
Applied biochemistry and biotechnology 2010 v.162 no.7 pp. 2098-2110
ISSN:
0273-2289
Subject:
proteins; immobilized enzymes; storage time; triacylglycerol lipase; methodology; genes; binding sites
Abstract:
... The presented work introduces a novel method to immobilize enzymes either purified or directly out of a crude extract onto magnetic particles in the micrometer range. This method is based on the creation of a fusion protein consisting of the enzyme of choice and a mutant dehalogenase. The dehalogenase gene is commercially available from the company Promega under the name HaloTagTM. When the fusion ...
DOI:
10.1007/s12010-010-8985-1
PubMed:
20473582

10. Facile Immobilization of Evolved Agrobacterium radiobacter Carbamoylase with High Thermal and Oxidative Stability

Author:
Chiang, C.J.; Chern, J.T.; Wang, J.Y.; Chao, Y.P.
Source:
Journal of agricultural and food chemistry 2008 v.56 no.15 pp. 6348-6354
ISSN:
0021-8561
Subject:
thermal stability; genetic transformation; microbial genetics; immobilized enzymes; Agrobacterium radiobacter; bioreactors; DNA; strain differences; mutation; amino acids; oxidative stability; binding sites; nucleotide sequences; mutants; synergism; amide hydrolases; enzyme activity; chitin; Escherichia coli
Abstract:
... Optically pure amino acids have been widely used as intermediates in the synthesis of antibiotics, antifungal agents, pesticides, and sweeteners. Of particular importance, D-p-hydroxyphenylglycine (D-HPG) can be produced from D,L-hydroxyphenly hydantoin (D,L-HPH) in a two-step reaction mediated by D-hydantoinase and N-carbamoyl-D-amino acid amidohydrolase (or carbamoylase). To make this production ...
DOI:
10.1021/jf8009365

11. Hydrophilic polyethylenimine modified magnetic graphene oxide composite as an efficient support for dextranase immobilization with improved stability and recyclable performance

Author:
Yajie Wang; Qiang Wang; Xiaoping Song; Jingjing Cai
Source:
Biochemical engineering journal 2019 v.141 pp. 163-172
ISSN:
1369-703X
Subject:
binding sites; dextranase; engineering; enzyme activity; graphene oxide; hydrophilicity; immobilized enzymes; magnetic separation; magnetism; nanosheets; pH; polyethyleneimine; temperature
Abstract:
... Development of appropriate support for enzyme immobilization with approving loading capacity, stability and recyclable performance is a significant topic in enzyme chemistry. In this study, the hydrophilic polyethylenimine (PEI) modified magnetic graphene oxide (GO) composite (Fe3O4@PDA@GO-PEI) was fabricated and acted as an efficient solid support for the immobilization of dextranase to improve t ...
DOI:
10.1016/j.bej.2018.10.015

12. Identification of ligands from natural products as inhibitors of glutathione S‐transferases using enzyme immobilized mesoporous magnetic beads with high‐performance liquid chromatography plus quadrupole time‐of‐flight mass spectrometry and molecular docking

Author:
Renxing Yi; Renjie Fu; Dapeng Li; Jin Qi; Haichun Liu
Source:
Journal of separation science 2019 v.42 no.24 pp. 3611-3620
ISSN:
1615-9306
Subject:
Perilla frutescens; binding capacity; binding sites; catalase; computer simulation; dopamine; drug therapy; enzyme inhibitors; glutathione transferase; green tea; high performance liquid chromatography; hydrogen; immobilized enzymes; leaves; ligands; magnetism; mass spectrometry; microparticles; multiple drug resistance; porous media; rosmarinic acid; silica; temperature
Abstract:
... Multidrug resistance is recognized as one of the main reasons leading to the failure of chemotherapy. Studies have shown that glutathione S‐transferase inhibitors could be regarded as multidrug resistance reversal agents. Herein, a method of applying enzyme immobilization, molecular docking, and high‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry was emp ...
DOI:
10.1002/jssc.201900318

13. Immobilization of endo-inulinase on poly-d-lysine coated CaCO3 micro-particles

Author:
Mahsan Karimi; Mehran Habibi-Rezaei; Mohammad Safari; Ali Akbar Moosavi-Movahedi; Maryam Sayyah; Rohollah Sadeghi; Jozef Kokini
Source:
Food research international 2014 v.66 pp. 485-492
ISSN:
0963-9969
Subject:
adsorption; amino acids; binding sites; calcium carbonate; chemical bonding; electrolytes; electrostatic interactions; fructose; half life; immobilized enzymes; inulinase; ion exchange; manufacturing; particle size; pharmaceutical industry; pyrolysis; storage quality; syrups; temperature; thermal stability; ultrasonics
Abstract:
... Inulinase is widely applied to produce high fructose syrup (HFS) which is used in the food and pharmaceutical industries. Numbers of different techniques such as physical adsorption, entrapment, ion exchange and covalent bonding have been used to immobilize enzymes on solid supports. In this study, an efficient technique for inulinase immobilization was developed which consists of adsorbing a cati ...
DOI:
10.1016/j.foodres.2014.08.041
CHORUS:
10.1016/j.foodres.2014.08.041

14. Modulating the Activity of Protein Conjugated to Gold Nanoparticles by Site-Directed Orientation and Surface Density of Bound Protein

Author:
Liu Feng; Wang Lei; Wang Hongwei; Yuan Lin; Li Jingwen; Brash John Law; Chen Hong
Source:
ACS Applied Materials & Interfaces 2015 v.7 no.6 pp. 3717-3724
ISSN:
1944-8252
Subject:
Escherichia coli; aqueous solutions; binding sites; bioactive properties; biocatalysis; cysteine; drugs; gold; immobilized enzymes; inorganic pyrophosphatase; models; mutants; nanogold; nanoparticles; protein content; site-directed mutagenesis
Abstract:
... The key property of protein–nanoparticle conjugates is the bioactivity of the protein. The ability to accurately modulate the activity of protein on the nanoparticles at the interfaces is important in many applications. In the work reported here, modulation of the activity of protein–gold nanoparticle (AuNP) conjugates by specifically orienting the protein and by varying the surface density of the ...
DOI:
10.1021/am5084545

15. Recent advances in metal-organic frameworks for separation and enrichment in proteomics analysis

Author:
Qianjing Liu; Nianrong Sun; Chun-hui Deng
Source:
Trends in analytical chemistry 2019 v.110 pp. 66-80
ISSN:
0165-9936
Subject:
adsorbents; binding sites; chemical species; coordination polymers; immobilized enzymes; ligands; peptides; proteins; proteomics; surface area
Abstract:
... Metal-organic frameworks (MOFs) have been broadly applied to sample preparation which is an essential step in proteomics research. Their large surface area provides abundant binding sites, their specific pore structure can exclude large-sized proteins and allow small molecules to get inside their channels. Besides, the flexible functionalization gives infinite possibilities of MOFs applications in ...
DOI:
10.1016/j.trac.2018.10.033

16. Screening of inhibitors of glycogen synthase kinase-3β from traditional Chinese medicines using enzyme-immobilized magnetic beads combined with high-performance liquid chromatography

Author:
Yunfang Li; Jia Xu; Yu Chen; Zhinan Mei; Yuxiu Xiao
Source:
Journal of chromatography 2015 v.1425 pp. 8-16
ISSN:
0021-9673
Subject:
Euonymus fortunei; Garcinia xanthochymus; Oriental traditional medicine; Prunus dulcis; binding sites; cost effectiveness; enzymatic reactions; enzyme activity; enzyme kinetics; high performance liquid chromatography; immobilized enzymes; inhibitory concentration 50; liquids; magnetism; molecular models; phosphates; screening; tau-protein kinase; tyrosine
Abstract:
... Glycogen synthase kinase-3β (GSK-3β) was immobilized on magnetic beads (MBs) by affinity method for the first time. The enzyme-immobilized MBs were coupled with high-performance liquid chromatography-ultraviolet (HPLC-UV) technique to establish a cost-effective and reliable method for screening of inhibitors of GSK-3β. A peptide substrate of GSK-3β containing a tyrosine residue was employed since ...
DOI:
10.1016/j.chroma.2015.10.062

17. Study of Molecular Conformation and Activity-Related Properties of Lipase Immobilized onto Core–Shell Structured Polyacrylic Acid-Coated Magnetic Silica Nanocomposite Particles

Author:
Esmaeilnejad-Ahranjani Parvaneh; Kazemeini Mohammad; Singh Gurvinder; Arpanaei Ayyoob
Source:
Langmuir 2016 v.32 no.13 pp. 3242-3252
ISSN:
1520-5827
Subject:
Fourier transform infrared spectroscopy; X-ray diffraction; acrylic acid; binding sites; carboxylic ester hydrolases; catalytic activity; chemical bonding; circular dichroism spectroscopy; immobilized enzymes; iron oxides; magnetism; molecular conformation; nanocomposites; pH; polyacrylic acid; silica; temperature; thermogravimetry; transmission electron microscopy; zeta potential
Abstract:
... A facile approach for the preparation of core–shell structured poly(acrylic acid) (PAA)-coated Fe₃O₄ cluster@SiO₂ nanocomposite particles as the support materials for the lipase immobilization is reported. Low- or high-molecular-weight (1800 and 100 000, respectively) PAA molecules were covalently attached onto the surface of amine-functionalized magnetic silica nanoacomposite particles. The succe ...
DOI:
10.1021/acs.langmuir.5b03614

18. Surface immobilization of a protease through an inhibitor-derived affinity ligand: a bioactive surface with defensive properties against an inhibitor

Author:
Zengchao Tang; Yafei Luan; Dan Li; Hui Du; David M. Haddleton; Hong Chen
Source:
Chemical communications 2015 v.51 no.75 pp. 14263-14266
ISSN:
1364-548X
Subject:
adsorption; binding sites; chemical bonding; chemical reactions; enzyme activity; immobilized enzymes; ligands; peptides; proteinases
Abstract:
... The concept of enzyme immobilization via an inhibitor-derived peptide was developed. This method of immobilization was shown to be advantageous over physical adsorption and covalent bonding in retaining the enzymatic activity. Moreover, the surface-immobilized enzyme exhibited resistance against its inhibitor due to the occupation of an inhibitor binding site on the enzyme. ...
DOI:
10.1039/c5cc05652g