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- Bruno Henrique de Oliveira; Gilberto Victor Coradi; Pedro de Oliva-Neto; Valeria Marta Gomes do Nascimento
- Industrial crops and products 2020 v.147 pp. 112235
- Crambe hispanica; Fusarium; biocatalysis; biocatalysts; carboxylic ester hydrolases; crambe cake; growing media; solid state fermentation; solvents; submerged fermentation; temperature; value added
- ... Lipases are promising tools for the synthesis of high value-added compounds and have traditionally been obtained from submerged fermentation processes (SmF). However, solid-state fermentation (SSF) allows linking the fermentation process directly to the enzyme application. Using fermented substrate as the biocatalysts may combine the steps of enzyme production, extraction, purification and immobil ...
- Katja Zorn; Isabel Oroz-Guinea; Uwe T. Bornscheuer
- Process biochemistry 2019 v.79 pp. 65-73
- Crambe abyssinica; Pseudozyma antarctica; biocatalysts; carboxylic ester hydrolases; crambe seed oil; erucic acid; esterification; esters; hydrolysis; industry; mutants; protein engineering; temperature profiles
- ... Erucic acid (C22:1, Δ13Z) is an interesting building block for the oleochemical industry and is abundantly present in Crambe abyssinica seed oil (∼59% of C22:1). For the enrichment of fatty acids (FA), lipases can be employed and we have found in a previous protein engineering study suitable variants of lipase A from Moesziomyces antarcticus (CAL-A) for the enrichment of C22:1 from Crambe oil deri ...