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- Da Som No; TingTing Zhao; Byung Hee Kim; Hee-Don Choi; In-Hwan Kim
- Journal of Molecular Catalysis. B, Enzymatic 2013 v.87 pp. 6-10
- Pseudozyma antarctica; biocatalysts; catalytic activity; crambe seed oil; erucic acid; ethanol; ethanolysis; temperature; triacylglycerols
- ... Erucic acid enrichment from crambe oil was accomplished in a recirculated packed bed (RPBR) reactor via ethanolysis using Novozym 435 lipase from Candida antarctica as a biocatalyst. The content of erucic acid of the crambe oil used was 59.8 mol% The erucic acid was located predominantly in the sn-1,3 position of the triacylglycerol of the crambe oil. Novozym 435 used in this study has selectivity ...
- Katja Zorn; Isabel Oroz-Guinea; Uwe T. Bornscheuer
- Process biochemistry 2019 v.79 pp. 65-73
- Crambe abyssinica; Pseudozyma antarctica; biocatalysts; carboxylic ester hydrolases; crambe seed oil; erucic acid; esterification; esters; hydrolysis; industry; mutants; protein engineering; temperature profiles
- ... Erucic acid (C22:1, Δ13Z) is an interesting building block for the oleochemical industry and is abundantly present in Crambe abyssinica seed oil (∼59% of C22:1). For the enrichment of fatty acids (FA), lipases can be employed and we have found in a previous protein engineering study suitable variants of lipase A from Moesziomyces antarcticus (CAL-A) for the enrichment of C22:1 from Crambe oil deri ...