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You searched for: Subject term biomass Remove constraint Subject term: biomass Subject term bacteria Remove constraint Subject term: bacteria Subject term anaerobic conditions Remove constraint Subject term: anaerobic conditions Subject term Sulfurospirillum Remove constraint Subject term: Sulfurospirillum
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1. Thermal proteome profiling allows quantitative assessment of interactions between tetrachloroethene reductive dehalogenase and trichloroethene

Author:
Dominique Türkowsky; Patrick Lohmann; Marie Mühlenbrink; Torsten Schubert; Lorenz Adrian; Tobias Goris; Nico Jehmlich; Martin von Bergen
Source:
Journal of proteomics 2019 v.192 pp. 10-17
ISSN:
1874-3919
Subject:
Sulfurospirillum; anaerobes; anaerobic conditions; bacteria; biomass; dehalogenation; enzyme substrates; eukaryotic cells; ligands; mass spectrometry; melting; oxidoreductases; proteins; proteome; protocols; substrate specificity; temperature; tetrachloroethylene; thermal stability
Abstract:
... Thermal proteome profiling (TPP) is increasingly applied in eukaryotes to investigate protein-ligand binding through protein melting curve shifts induced by the presence of a ligand. In anaerobic bacteria, identification of protein-substrate interactions is a major challenge. We applied TPP to Sulfurospirillum multivorans, which is able to use trichloroethene as electron acceptor for growth, to in ...
DOI:
10.1016/j.jprot.2018.05.018