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1. Bacterial cellulose: recent progress in production and industrial applications

Author:
Nermin Hande Avcioglu
Source:
World journal of microbiology & biotechnology 2022 v.38 no.5 pp. 86
ISSN:
0959-3993
Subject:
Acetobacter; Achromobacter; Agrobacterium; Azotobacter; Gluconacetobacter; Lactobacillus; arabinose; biodegradability; biopolymers; biotechnology; cellulose; crystal structure; hemicellulose; lignin; nanofibers; pectins; polymerization; porosity; surface area; tensile strength
Abstract:
... Bacterial cellulose (BC) is synthesized as a valuable extracellular biopolymer by several bacteria belonging to the genera of Acetobacter, Achromobacter, Komagataeibacter, Agrobacterium, Bacillus, Azotobacter, Sarcinia, Lactobacillus and Gluconacetobacter. Unlike plant cellulose, since BC does not contain lignin, hemicellulose, pectin, arabinose and other plant-derived contaminants, it can be obta ...
DOI:
10.1007/s11274-022-03271-y

2. Co-expressing Leucine Responsive Regulatory protein (Lrp) enhances recombinant L-Asparaginase-II production in Escherichia coli

Author:
Shubhashree Mahalik; Ashish Sharma; Dibya Ranjan Das; Debashrita Mittra; Krishna Jyoti Mukherjee
Source:
Journal of biotechnology 2022 v.351 pp. 99-108
ISSN:
0168-1656
Subject:
Escherichia coli; amino acid metabolism; arabinose; asparaginase; biotechnology; leucine; plasmids; protein synthesis; recombinant proteins; regulatory proteins; stress response; transcriptomics
Abstract:
... Over expression of recombinant proteins triggers a cellular stress response (CSR) that down-regulates numerous genes that have a key role in sustaining expression. Instead of trying to individually up-regulate these genes we hypothesized that a superior strategy would be to modulate the expression of global regulators that control the expression of many such downstream genes. Transcriptomic profil ...
DOI:
10.1016/j.jbiotec.2022.04.012

3. Lactic acid production from food waste using the lactogenic Escherichia coli strain JU15: optimization of reducing sugar recovery

Author:
Anaid M Santos‐Corona; Pedro E Lázaro‐Mixteco; Ana Alejandra Vargas‐Tah; Agustín J Castro‐Montoya
Source:
Journal of chemical technology and biotechnology 2022 v.97 no.3 pp. 668-675
ISSN:
0268-2575
Subject:
Escherichia coli; arabinose; biodegradability; biotechnology; feedstocks; fermentation; food waste; fructose; glucose; hydrolysis; polylactic acid; response surface methodology
Abstract:
... BACKGROUND: d‐Lactic acid (DLA) has numerous industrial applications, including production of biodegradable polylactic acid, which is one of the most promising biodegradable polymers. Looking for alternative feedstocks is a high priority and recent focus has been on its biological synthesis and biotechnological valorization from food waste (FW) materials. In this study, a response surface methodol ...
DOI:
10.1002/jctb.6949

4. Polysaccharides from Tetrastigma hemsleyanum Diels et Gilg: optimum extraction, monosaccharide compositions, and antioxidant activity

Author:
Ling Sun; Jing-Jing Lu; Bi-Xu Wang; Tong Sun; Bing-Qi Zhu; Zhi-Shan Ding; Fang-Mei Zhou; Qian-Xing Jin
Source:
Preparative biochemistry & biotechnology 2022 v.52 no.4 pp. 383-393
ISSN:
1532-2297
Subject:
Tetrastigma; antioxidant activity; arabinose; biotechnology; dose response; endo-1,4-beta-glucanase; fluorescence recovery after photobleaching; galactose; galacturonic acid; glucose; glucuronic acid; hydroxyl radicals; mannose; medicine; polysaccharides; response surface methodology; rhamnose; ultrasonics
Abstract:
... The optimization of extraction of Tetrastigma hemsleyanum Diels et Gilg polysaccharides (THP) using ultrasonic with enzyme method and its monosaccharide compositions and antioxidant activity were investigated in this work. Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (XA) dosage of cellulas ...
DOI:
10.1080/10826068.2021.1952600

5. Alternative Heterologous Expression of L-Arabinose Isomerase from Enterococcus faecium DBFIQ E36 By Residual Whey Lactose Induction

Author:
Ticiane C. de Souza; Ravena Casemiro Oliveira; Saulo Gonçalves Santiago Bezerra; Ricardo M. Manzo; Enrique J. Mammarella; Denise Cavalcante Hissa; Luciana R. B. Gonçalves
Source:
Molecular biotechnology 2021 v.63 no.4 pp. 289-304
ISSN:
1073-6085
Subject:
Enterococcus faecium; affinity chromatography; arabinose; biotechnology; biotransformation; carbon; catalytic activity; enzyme activity; galactose; glucose; glycerol; heterologous gene expression; isomerases; lactose; tagatose; whey
Abstract:
... This study reports an alternative strategy for the expression of a recombinant L-AI from Enterococcus faecium DBFIQ E36 by auto-induction using glucose and glycerol as carbon sources and residual whey lactose as inducer agent. Commercial lactose and isopropyl β-D-1-thiogalactopyranoside (IPTG) were also evaluated as inducers for comparison of enzyme expression levels. The enzymatic extracts were p ...
DOI:
10.1007/s12033-021-00301-2

6. An overview of D-galactose utilization through microbial fermentation and enzyme-catalyzed conversion

Author:
Qiuming Chen; Wei Xu; Hao Wu; Cuie Guang; Wenli Zhang; Wanmeng Mu
Source:
Applied microbiology and biotechnology 2021 v.105 no.19 pp. 7161-7170
ISSN:
0175-7598
Subject:
arabinose; biofuels; biomass; biotechnology; biotransformation; fermentation; fuel production; galactose; genetic engineering; isomerases; macroalgae; microbiology; people; raw materials; tagatose
Abstract:
... D-Galactose is an abundant carbohydrate monomer in nature and widely exists in macroalgae, plants, and dairy wastes. D-Galactose is useful as a raw material for biomass fuel production or low-calorie sweetener production, attracting increased attention. This article summarizes the studies on biotechnological processes for galactose utilization. Two main research directions of microbial fermentatio ...
DOI:
10.1007/s00253-021-11568-5

7. Biorefinery potential of sustainable municipal wastewater treatment using fast-growing willow

Author:
E. Sas; L.M. Hennequin; A. Frémont; A. Jerbi; N. Legault; J. Lamontagne; N. Fagoaga; M. Sarrazin; J.P. Hallett; P.S. Fennell; S. Barnabé; M. Labrecque; N.J.B. Brereton; F.E. Pitre
Source:
Science of the total environment 2021 v.792 pp. 148146
ISSN:
0048-9697
Subject:
arabinose; bioenergy; biomass production; biorefining; biotechnology; cell walls; economic feasibility; environment; flavonoids; galactose; glucans; glucose; ionic liquids; lignans; lignin; lignin content; lignocellulose; mannose; metabolites; municipal wastewater; phenotype; saccharification; salt tolerance; wastewater irrigation; wastewater treatment; xylose
Abstract:
... The use of willow plantations can be a sustainable approach for treating primary municipal wastewater, potentially reducing both the environmental and economic burdens associated with conventional treatment. However, the impact of wastewater irrigation upon the willow biorefinery potential has not yet been established. To investigate this effect, three-year-old field grown willows were harvested f ...
DOI:
10.1016/j.scitotenv.2021.148146

8. CAZymes from the thermophilic fungus Thermoascus aurantiacus are induced by C5 and C6 sugars

Author:
Raphael Gabriel; Rebecca Mueller; Lena Floerl; Cynthia Hopson; Simon Harth; Timo Schuerg; Andre Fleissner; Steven W. Singer
Source:
Biotechnology for biofuels 2021 v.14 no.1 pp. 169
ISSN:
1754-6834
Subject:
arabinose; biofuels; biotechnology; cellobiose; endo-1,4-beta-glucanase; esterases; gene expression; heat tolerance; lignocellulose; sequence analysis; thermal stability; thermophilic fungi; unfolded protein response; xylose
Abstract:
... BACKGROUND: Filamentous fungi are excellent lignocellulose degraders, which they achieve through producing carbohydrate active enzymes (CAZymes). CAZyme production is highly orchestrated and gene expression analysis has greatly expanded understanding of this important biotechnological process. The thermophilic fungus Thermoascus aurantiacus secretes highly active thermostable enzymes that enable s ...
DOI:
10.1186/s13068-021-02018-5

9. Enhanced Enzymatic Hydrolysis and Structure Properties of Bamboo by Moderate Two-Step Pretreatment

Author:
Jing Yang; Hao Xu; Jianchun Jiang; Ning Zhang; Jingcong Xie; Jian Zhao; Min Wei
Source:
Applied biochemistry and biotechnology 2021 v.193 no.4 pp. 1011-1022
ISSN:
0273-2289
Subject:
ambient temperature; arabinose; bamboos; biotechnology; delignification; endo-1,4-beta-glucanase; enzymatic hydrolysis; glucose; polysaccharides; saccharification; xylose
Abstract:
... A moderate two-step pretreatment method was investigated to improve the enzymatic saccharification of bamboo residues. SEM and FTIR were employed to characterize the structure changes. Fed-batch enzymatic saccharification was performed to obtain high concentration of fermentable sugar. Bamboo was impregnated at low severity of conditions (room temperature, 2% H₂SO₄ or 2% NaOH, 48 h) to initially a ...
DOI:
10.1007/s12010-020-03472-x

10. Extraction of sugarcane bagasse arabinoxylan, integrated with enzymatic production of xylo-oligosaccharides and separation of cellulose

Author:
Leila Khaleghipour; Javier A. Linares-Pastén; Hamid Rashedi; Seyed Omid Ranaei Siadat; Andrius Jasilionis; Said Al-Hamimi; Roya R. R. Sardari; Eva Nordberg Karlsson
Source:
Biotechnology for biofuels 2021 v.14 no.1 pp. 153
ISSN:
1754-6834
Subject:
Bacillus halodurans; alkalinity; arabinose; arabinoxylan; biofuels; biotechnology; cellulose; endo-1,4-beta-xylanase; enzymatic hydrolysis; glycosides; hydrolysis; lignin; liquids; molecular weight; solubilization; sugar content; sugarcane; sugarcane bagasse; temperature; value added; xylan; xylooligosaccharides; xylose
Abstract:
... Sugarcane processing roughly generates 54 million tonnes sugarcane bagasse (SCB)/year, making SCB an important material for upgrading to value-added molecules. In this study, an integrated scheme was developed for separating xylan, lignin and cellulose, followed by production of xylo-oligosaccharides (XOS) from SCB. Xylan extraction conditions were screened in: (1) single extractions in NaOH (0.25 ...
DOI:
10.1186/s13068-021-01993-z

11. Herbaspirillum seropedicae expresses non-phosphorylative pathways for D-xylose catabolism

Author:
Ana Karen Malán; Thalita Tuleski; Ana Inés Catalán; Emanuel Maltempi de Souza; Silvia Batista
Source:
Applied microbiology and biotechnology 2021 v.105 no.19 pp. 7339-7352
ISSN:
0175-7598
Subject:
Herbaspirillum seropedicae; arabinose; biotechnology; carbon; catabolism; endophytes; genes; hexoses; mutants; oxidoreductases; pentoses; poly-3-hydroxybutyrate; pyruvic acid; sequence analysis; xylose
Abstract:
... Herbaspirillum seropedicae is a β-proteobacterium that establishes as an endophyte in various plants. These bacteria can consume diverse carbon sources, including hexoses and pentoses like D-xylose. D-xylose catabolic pathways have been described in some microorganisms, but databases of genes involved in these routes are limited. This is of special interest in biotechnology, considering that D-xyl ...
DOI:
10.1007/s00253-021-11507-4

12. Hypolipidemic effect of soluble dietary fibers prepared from Asparagus officinalis and their effects on the modulation of intestinal microbiota

Author:
Miansong Zhang; Mengke Yao; Airong Jia; Yaping Shi; Xinfeng Bai; Xue Liu; Tingting Cui; Xin Liu; Changheng Liu
Source:
Food science and biotechnology 2021 v.30 no.13 pp. 1721-1731
ISSN:
1226-7708
Subject:
Asparagus officinalis; Bacteroides; Firmicutes; Proteobacteria; antioxidants; arabinose; biotechnology; blood lipids; blood serum; dietary fiber; food science; galactose; galacturonic acid; genes; glucose; high performance liquid chromatography; intestinal microorganisms; lipemic effect; low density lipoprotein cholesterol; mannose; molecular weight; oral administration; polysaccharides; rhamnose; spray drying; triacylglycerols
Abstract:
... The soluble dietary fiber from Asparagus officinalis (ASDF) was successively prepared using enzymolysis combined with spray-drying technology. High-performance liquid chromatography analysis showed that ASDF contained two polysaccharide fractions with the average molecular weight of 2.77 × 10⁵ and 6.44 × 10³ Da, and was composed of mannose, rhamnose, galacturonic acid, glucose, galactose, and arab ...
DOI:
10.1007/s10068-021-01001-y

13. Lentinan administration alleviates diarrhea of rotavirus-infected weaned pigs via regulating intestinal immunity

Author:
Xiangqi Fan; Haiyan Hu; Daiwen Chen; Bing Yu; Jun He; Jie Yu; Junqiu Luo; Erik Eckhardt; Yuheng Luo; Jianping Wang; Hui Yan; Xiangbing Mao
Source:
Journal of animal science and biotechnology 2021 v.12 no.1 pp. 43
ISSN:
2049-1891
Subject:
animal science; arabinose; biotechnology; cell viability; diarrhea; galactose; glucose; growth performance; humans; immunity; intestines; molecular weight; peptides; polysaccharides
Abstract:
... BACKGROUND: Lentinan (LNT) may regulate many important physiological functions of human and animals. This study aimed to verify whether LNT administration could relieve diarrhea via improving gut immunity in rotavirus (RV)-challenged weaned pigs. METHODS: Twenty-eight weaned pigs were randomly fed 2 diets containing 0 or 84 mg/kg LNT product for 19 d (n = 14). RV infection was executed on d 15. Af ...
DOI:
10.1186/s40104-021-00562-6

14. Lignocellulose-Degrading Enzymes: A Biotechnology Platform for Ferulic Acid Production from Agro-Industrial Side Streams

Author:
Vitalijs Radenkovs; Karina Juhnevica-Radenkova; Jorens Kviesis; Danija Lazdina; Anda Valdovska; Fernando Vallejo; Gunars Lacis
Source:
Foods 2021 v.10 no.12 pp. -
ISSN:
2304-8158
Subject:
affordability; arabinose; bioprocessing; biorefining; biotechnology; carbon dioxide; enzymatic hydrolysis; ethanol; ferulic acid; glucose; high performance liquid chromatography; lignocellulose; lignocellulosic wastes; mass spectrometry; oat bran; polymers; refractive index; rye bran; solid phase extraction; supercritical fluid extraction; wheat; xylose
Abstract:
... Biorefining by enzymatic hydrolysis (EH) of lignocellulosic waste material due to low costs and affordability has received enormous interest amongst scientists as a potential strategy suitable for the production of bioactive ingredients and chemicals. In this study, a sustainable and eco-friendly approach to extracting bound ferulic acid (FA) was demonstrated using single-step EH by a mixture of l ...
DOI:
10.3390/foods10123056

15. Optimization of xylitol production from xylose by a novel arabitol limited co-producing Barnettozyma populi NRRL Y-12728

Author:
Badal C. Saha; Gregory J. Kennedy
Source:
Preparative biochemistry & biotechnology 2021 v.51 no.8 pp. 761-768
ISSN:
1532-2297
Subject:
acetic acid; aeration; arabinose; arabitol; biotechnology; corn stover; ethanol; fermentation; glucose; health promotion; hydrolysates; pH; response surface methodology; xylitol; xylose; yeasts
Abstract:
... Xylitol is a widely marketed sweetener with good functionality and health-promoting properties. It can be synthetized by many yeast species in a one-step reduction of xylose. Arabinose is a common contaminant found in xylose and there is ongoing interest in finding biocatalysts that selectively produce xyltiol. From a screen of 99 yeasts, Barnettozyma populi Y-12728 was found to selectively produc ...
DOI:
10.1080/10826068.2020.1855443

16. Overexpression of the rice BAHD acyltransferase AT10 increases xylan-bound p-coumarate and reduces lignin in Sorghum bicolor

Author:
Yang Tian; Chien-Yuan Lin; Joon-Hyun Park; Chuan-Yin Wu; Ramu Kakumanu; Venkataramana R. Pidatala; Khanh M. Vuu; Alberto Rodriguez; Patrick M. Shih; Edward E. K. Baidoo; Stephen Temple; Blake A. Simmons; John M. Gladden; Henrik V. Scheller; Aymerick Eudes
Source:
Biotechnology for biofuels 2021 v.14 no.1 pp. 217
ISSN:
1754-6834
Subject:
Panicum virgatum; Sorghum bicolor; alkaline hydrolysis; annuals; arabinose; biofuels; biomass; biotechnology; cell walls; cellulose microfibrils; crosslinking; digestibility; energy crops; feedstocks; genetically modified organisms; glucose; ionic liquids; lignification; lignin; lignin content; phylogeny; rice; saccharification; xylan; xylose
Abstract:
... BACKGROUND: The development of bioenergy crops with reduced recalcitrance to enzymatic degradation represents an important challenge to enable the sustainable production of advanced biofuels and bioproducts. Biomass recalcitrance is partly attributed to the complex structure of plant cell walls inside which cellulose microfibrils are protected by a network of hemicellulosic xylan chains that cross ...
DOI:
10.1186/s13068-021-02068-9

17. Structure-based substrate specificity analysis of GH11 xylanase from Streptomyces olivaceoviridis E-86

Author:
Zui Fujimoto; Naomi Kishine; Koji Teramoto; Sosyu Tsutsui; Satoshi Kaneko
Source:
Applied microbiology and biotechnology 2021 v.105 no.5 pp. 1943-1952
ISSN:
0175-7598
Subject:
Streptomyces olivaceoviridis; arabinose; bagasse; biotechnology; crystal structure; electrospray ionization mass spectrometry; energy; face; glucuronic acid; glycosidic linkages; mechanism of action; microbiology; nuclear magnetic resonance spectroscopy; substrate specificity; topology; xylan; xylanases; xylooligosaccharides; xylose
Abstract:
... Although many xylanases have been studied, many of the characteristics of xylanases toward branches in xylan remain unclear. In this study, the substrate specificity of a GH11 xylanase from Streptomyces olivaceoviridis E-86 (SoXyn11B) was elucidated based on its three-dimensional structure. Subsite mapping suggests that SoXyn11B has seven subsites (four subsites on the – side and three subsites on ...
DOI:
10.1007/s00253-021-11098-0

18. Substrate Specificities of GH8, GH39, and GH52 β-xylosidases from Bacillus halodurans C-125 Toward Substituted Xylooligosaccharides

Author:
Koji Teramoto; Sosyu Tsutsui; Tomoko Sato; Zui Fujimoto; Satoshi Kaneko
Source:
Applied biochemistry and biotechnology 2021 v.193 no.4 pp. 1042-1055
ISSN:
0273-2289
Subject:
Bacillus halodurans; arabinose; biotechnology; enzyme activity; glucuronic acid; glycosides; hydrogen bonding; xylooligosaccharides; xylose
Abstract:
... Substrate specificities of glycoside hydrolase families 8 (Rex), 39 (BhXyl39), and 52 (BhXyl52) β-xylosidases from Bacillus halodurans C-125 were investigated. BhXyl39 hydrolyzed xylotriose most efficiently among the linear xylooligosaccharides. The activity decreased in the order of xylohexaose > xylopentaose > xylotetraose and it had little effect on xylobiose. In contrast, BhXyl52 hydrolyzed xy ...
DOI:
10.1007/s12010-020-03451-2

19. The chimeric GaaR-XlnR transcription factor induces pectinolytic activities in the presence of D-xylose in Aspergillus niger

Author:
Roland S. Kun; Sandra Garrigues; Marcos Di Falco; Adrian Tsang; Ronald P. de Vries
Source:
Applied microbiology and biotechnology 2021 v.105 no.13 pp. 5553-5564
ISSN:
0175-7598
Subject:
Aspergillus niger; CRISPR-Cas systems; arabinose; biotechnology; fungi; galacturonic acid; mutants; pectins; proteomics; transactivators; xylose
Abstract:
... Aspergillus niger is a filamentous fungus well known for its ability to produce a wide variety of pectinolytic enzymes, which have many applications in the industry. The transcriptional activator GaaR is induced by 2-keto-3-deoxy-L-galactonate, a compound derived from D-galacturonic acid, and plays a major role in the regulation of pectinolytic genes. The requirement for inducer molecules can be a ...
DOI:
10.1007/s00253-021-11428-2

20. The potential of extracellular biopolymer production by Mesorhizobium sp. from monosaccharide constituents of lignocellulosic biomass

Author:
Belkis Chalup Silveira Roesler; Renata Gonçalves Vaz; Tereza Cristina Luque Castellane; Eliana Gertrudes de Macedo Lemos; Carlos André Veiga Burkert
Source:
Biotechnology letters 2021 v.43 no.7 pp. 1385-1394
ISSN:
0141-5492
Subject:
Mesorhizobium; arabinose; biomass; biopolymers; biotechnology; carbon; exopolysaccharides; glucose; glucuronic acid; hydrolysates; lignocellulose; rhamnose; soybeans; xylose
Abstract:
... OBJECTIVE: The effects of monosaccharide constituents of lignocellulosic materials on exopolysaccharide (EPS) production by Mesorhizobium sp. Semia 816 were studied. RESULTS: According to the results, by using sugars commonly found in lignocellulosic biomass as carbon sources (glucose, arabinose and xylose), no significant differences were observed in the production of EPS, reaching 3.39 g/L, 3.33 ...
DOI:
10.1007/s10529-021-03119-9

21. Whole Cell Biotransformation of 1-dodecanol by Escherichia coli by Soluble Expression of ADH Enzyme from Yarrowia lipolytica

Author:
Ji-Hwan Jang; Kwon-Young Choi
Source:
Biotechnology and bioprocess engineering 2021 v.26 no.2 pp. 247-255
ISSN:
1226-8372
Subject:
Escherichia coli; Yarrowia lipolytica; alcohol dehydrogenase; arabinose; bioprocessing; biotechnology; biotransformation; molecular chaperones; oxidation; temperature; tetracycline; thermodynamics
Abstract:
... In this study, alcohol dehydrogenases (ADH) enzymes from Yarrowia lipolytica were investigated for the cloning, soluble expression, and biotransformation of 1-dodecanol to 1-dodecanal, which reaction was thermodynamically unfavorable. Sole expression of ADHs in Escherichia coli did not produce soluble form of cytosolic protein, in spite of the effort to solubilize ADH protein by optimizing IPTG co ...
DOI:
10.1007/s12257-020-0176-5

22. Yeast based biorefineries for oleochemical production

Author:
Yiming Zhang; Jens Nielsen; Zihe Liu
Source:
Current opinion in biotechnology 2021 v.67 pp. 26-34
ISSN:
0958-1669
Subject:
arabinose; biorefining; biosynthesis; biotechnology; carbon metabolism; feedstocks; lipid metabolism; xylose; yeasts
Abstract:
... Biosynthesis of oleochemicals enables sustainable production of natural and unnatural alternatives from renewable feedstocks. Yeast cell factories have been extensively studied and engineered to produce a variety of oleochemicals, focusing on both central carbon metabolism and lipid metabolism. Here, we review recent progress towards oleochemical synthesis in yeast based biorefineries, as well as ...
DOI:
10.1016/j.copbio.2020.11.009

23. An Arabinoxylan Extracted from Corn Grain Is Inhibitory to Cellulase Activity

Author:
Hong Fang; Elizabeth E. Hood
Source:
Industrial biotechnology 2020 v.16 no.5 pp. 290-299
ISSN:
1931-8421
Subject:
arabinose; arabinoxylan; biotechnology; corn; endo-1,4-beta-glucanase; heat; molecular weight; protein synthesis; purification methods; recombinant proteins; sampling; thermal stability; water solubility; xylose
Abstract:
... The maize seed expression system is a powerful platform for recombinant protein production. It has been utilized for high-yield production of industrial cellulases such as endo-1,4-β-D-glucanase (E1) and 1,4-β-D-glucan cellobiohydrolases (CBHI & CBHII). However, inhibitory compounds that could significantly reduce the cellulolytic efficiency were detected even in purified E1 and CBHII samples. Aft ...
DOI:
10.1089/ind.2020.0009

24. Characterization and functional analysis of two novel thermotolerant α-L-arabinofuranosidases belonging to glycoside hydrolase family 51 from Thielavia terrestris and family 62 from Eupenicillium parvum

Author:
Liangkun Long; Lu Sun; Qunying Lin; Shaojun Ding; Franz J. St John
Source:
Applied microbiology and biotechnology 2020 v.104 no.20 pp. 8719-8733
ISSN:
0175-7598
Subject:
Eupenicillium; Thielavia; arabinans; arabinose; arabinoxylan; biotechnology; buffer index; calcium; enzymatic hydrolysis; glycosides; heat tolerance; hydrolysis; microbiology; pH; rye; sugar beet; temperature; thermal stability; wheat; xylan; xylanases; xylose
Abstract:
... Arabinofuranose substitutions on xylan are known to interfere with enzymatic hydrolysis of this primary hemicellulose. In this work, two novel α-L-arabinofuranosidases (ABFs), TtABF51A from Thielavia terrestris and EpABF62C from Eupenicillium parvum, were characterized and functionally analyzed. From sequences analyses, TtABF51A and EpABF62C belong to glycoside hydrolase (GH) families 51 and 62, r ...
DOI:
10.1007/s00253-020-10867-7

25. Characterization of an L-Arabinose Isomerase from Bacillus velezensis and Its Application for L-Ribulose and L-Ribose Biosynthesis

Author:
Zongren Guo; Liangkun Long; Shaojun Ding
Source:
Applied biochemistry and biotechnology 2020 v.192 no.3 pp. 935-951
ISSN:
0273-2289
Subject:
Bacillus velezensis; Escherichia coli; arabinose; biosynthesis; biotechnology; isomerases; pH; ribulose
Abstract:
... L-Ribulose and L-ribose are two high-value unnatural sugars that can be biosynthesized by sugar isomerases. In this paper, an L-arabinose isomerase (BvAI) from Bacillus velezensis CICC 24777 was cloned and overexpressed in Escherichia coli BL21 (DE3) strain. The maximum activity of recombinant BvAI was observed at 45 °C and pH 8.0, in the presence of 1.0 mM Mn²⁺. Approximately 207.2 g/L L-ribulose ...
DOI:
10.1007/s12010-020-03380-0

26. Characterization of highly active 2-keto-3-deoxy-L-arabinonate and 2-keto-3-deoxy-D-xylonate dehydratases in terms of the biotransformation of hemicellulose sugars to chemicals

Author:
Samuel Sutiono; Bettina Siebers; Volker Sieber
Source:
Applied microbiology and biotechnology 2020 v.104 no.16 pp. 7023-7035
ISSN:
0175-7598
Subject:
Cupriavidus necator; Escherichia coli; Pseudomonas putida; arabinose; biotechnology; biotransformation; enzymes; hemicellulose; magnesium; microbiology; stereoisomers; xylose
Abstract:
... 2-keto-3-L-arabinonate dehydratase (L-KdpD) and 2-keto-3-D-xylonate dehydratase (D-KdpD) are the third enzymes in the Weimberg pathway catalyzing the dehydration of respective 2-keto-3-deoxy sugar acids (KDP) to α-ketoglutaric semialdehyde (KGSA). The Weimberg pathway has been explored recently with respect to the synthesis of chemicals from L-arabinose and D-xylose. However, only limited work has ...
DOI:
10.1007/s00253-020-10742-5

27. Characterization of two extracellular arabinanases in Lactobacillus crispatus

Author:
Qing Li; Michael G. Gänzle
Source:
Applied microbiology and biotechnology 2020 v.104 no.23 pp. 10091-10103
ISSN:
0175-7598
Subject:
Lactobacillus crispatus; acetates; affinity chromatography; amylases; arabinans; arabinose; biotechnology; carbon; fructans; lactic acid; metabolism; operon; pH; pectins; pentose phosphate cycle; phosphoketolase; probiotics; starch; sugar beet; temperature
Abstract:
... Extracellular glycosyl hydrolases are uncommon in lactobacilli and include amylases and fructosidases mediating starch and fructan utilization, respectively. Extracellular arabinanases have not been described in lactobacilli. This study is aimed at identifying the function of an arabinan utilization operon in Lactobacillus crispatus DSM29598 and at characterizing two putative extracellular arabina ...
DOI:
10.1007/s00253-020-10979-0

28. Construction of a novel sugar alcohol-inducible expression system in Bacillus licheniformis

Author:
Fengxu Xiao; Youran Li; Yupeng Zhang; Hanrong Wang; Liang Zhang; Zhongyang Ding; Zhenghua Gu; Sha Xu; Guiyang Shi
Source:
Applied microbiology and biotechnology 2020 v.104 no.12 pp. 5409-5425
ISSN:
0175-7598
Subject:
Bacillus licheniformis; arabinose; biomass; biotechnology; mannitol; mannose; microbiology; sorbitol; sorbose
Abstract:
... Bacillus licheniformis is an important industrial microorganism that can utilize a wide range of biomass. However, the lack of expression elements in B. licheniformis, especially regulated promoters, significantly restricts its applications. In this study, two promoters involved in the sugar alcohol uptake pathway, PmtlA and PmtlR, were characterized and developed as regulated promoters for expres ...
DOI:
10.1007/s00253-020-10618-8

29. Development of optimal steam explosion pretreatment and highly effective cell factory for bioconversion of grain vinegar residue to butanol

Author:
Menglei Xia; Mingmeng Peng; Danni Xue; Yang Cheng; Caixia Li; Di Wang; Kai Lu; Yu Zheng; Ting Xia; Jia song; Min Wang
Source:
Biotechnology for biofuels 2020 v.13 no.1 pp. 111
ISSN:
1754-6834
Subject:
Clostridium acetobutylicum; ambient temperature; arabinans; arabinose; biobutanol; biomass; biorefining; biotechnology; biotransformation; bran; butanol; chemical elements; diameter; domestication; enzymatic hydrolysis; equations; extraction; feedstocks; fields; glucans; glucose; grains; hydrolysates; kinetics; knowledge; lignocellulose; liquids; metabolic engineering; models; mutants; pretreatment; solid state fermentation; statistical analysis; steam; vinegars; water; xylan; xylose
Abstract:
... BACKGROUND: The industrial vinegar residue (VR) from solid-state fermentation, mainly cereals and their bran, will be a potential feedstock for future biofuels because of their low cost and easy availability. However, utilization of VR for butanol production has not been as much optimized as other sources of lignocellulose, which mainly stem from two key elements: (i) high biomass recalcitrance to ...
DOI:
10.1186/s13068-020-01751-7

30. Functional characterization of a novel thermophilic exo-arabinanase from Thermothielavioides terrestris

Author:
Josman Velasco; Bianca Oliva; Aline Larissa Gonçalves; Awana Silva Lima; Gislene Ferreira; Bruno Alves França; Evandro José Mulinari; Thiago Augusto Gonçalves; Fábio Márcio Squina; Marco Antonio Seiki Kadowaki; Alfredo Maiorano; Igor Polikarpov; Leandro Cristante de Oliveira; Fernando Segato
Source:
Applied microbiology and biotechnology 2020 v.104 no.19 pp. 8309-8326
ISSN:
0175-7598
Subject:
Aspergillus nidulans; arabinans; arabinose; biotechnology; capillary zone electrophoresis; depolymerization; glycoproteins; glycosides; glycosylation; hydrolases; pH; pectins; prebiotics; raw materials; small-angle X-ray scattering; sugar beet; sugar beet pulp; synergism; temperature; thermophilic fungi
Abstract:
... Arabinanases from glycoside hydrolase family GH93 are enzymes with exo-activity that hydrolyze the α-1,5 bonds between arabinose residues present on arabinan. Currently, several initiatives aiming to use byproducts rich in arabinan such as pectin and sugar beet pulp as raw material to produce various compounds of interest are being developed. However, it is necessary to use robust enzymes that hav ...
DOI:
10.1007/s00253-020-10806-6

31. High-level production of industrially relevant oxidases by a two-stage fed-batch approach: overcoming catabolite repression in arabinose-inducible Escherichia coli systems

Author:
Ramón Román; Nikola Lončar; Antoni Casablancas; Marco W. Fraaije; Glòria Gonzalez
Source:
Applied microbiology and biotechnology 2020 v.104 no.12 pp. 5337-5345
ISSN:
0175-7598
Subject:
Escherichia coli; arabinose; batch fermentation; biotechnology; carbon; catabolite repression; eugenol; glucose; glycerol; hydroxymethylfurfural; microbiology; oxidoreductases; protein synthesis
Abstract:
... With the growing interest in enzyme applications, there is an urgent demand for economic, affordable, and flexible enzyme production processes. In the present paper, we developed a high cell density fed-batch process for the production of two cofactor-containing oxidase, 5-hydroxymethylfurfural oxidase (HMFO) and eugenol oxidase (EUGO). The approach involved the arabinose-inducible system to drive ...
DOI:
10.1007/s00253-020-10622-y

32. Improved analysis of arabinoxylan-bound hydroxycinnamate conjugates in grass cell walls

Author:
Alexis Eugene; Catherine Lapierre; John Ralph
Source:
Biotechnology for biofuels 2020 v.13 no.1 pp. 202
ISSN:
1754-6834
Subject:
acidolysis; acylation; arabinose; arabinoxylan; biofuels; biomass; biotechnology; branching; cell walls; crosslinking; esters; ferulic acid; grasses; hydrolysis; incidence; isomers; lignin; methanolysis; p-coumaric acid; protocols; tissues; xylan; yields
Abstract:
... BACKGROUND: Arabinoxylan in grass cell walls is acylated to varying extents by ferulate and p-coumarate at the 5-hydroxy position of arabinosyl residues branching off the xylan backbone. Some of these hydroxycinnamate units may then become involved in cell wall radical coupling reactions, resulting in ether and other linkages amongst themselves or to monolignols or oligolignols, thereby crosslinki ...
DOI:
10.1186/s13068-020-01841-6

33. Insights into the cellulose degradation mechanism of the thermophilic fungus Chaetomium thermophilum based on integrated functional omics

Author:
Xin Li; Chao Han; Weiguang Li; Guanjun Chen; Lushan Wang
Source:
Biotechnology for biofuels 2020 v.13 no.1 pp. 143
ISSN:
1754-6834
Subject:
Chaetomium; arabinose; binding capacity; biofuels; biomass; biotechnology; carbon; cellobiose dehydrogenase; cellulose; degradation; design; genes; habitats; industrial applications; lignocellulose; secretion; thermophilic fungi; xylanases
Abstract:
... BACKGROUND: Lignocellulose is the most abundant and renewable biomass resource on the planet. Lignocellulose can be converted into biofuels and high-value compounds; however, its recalcitrance makes its breakdown a challenge. Lytic polysaccharide monooxygenases (LPMOs) offer tremendous promise for the degradation of recalcitrant polysaccharides. Chaetomium thermophilum, having many LPMO-coding gen ...
DOI:
10.1186/s13068-020-01783-z

34. Loss of TaIRX9b gene function in wheat decreases chain length and amount of arabinoxylan in grain but increases cross‐linking

Author:
Till K. Pellny; Archana Patil; Abigail J. Wood; Jackie Freeman; Kirstie Halsey; Amy Plummer; Ondrej Kosik; Henry Temple; Joel D. Collins; Paul Dupree; Simon Berry; Peter R. Shewry; Alison Lovegrove; Andrew L. Phillips; Rowan A.C. Mitchell
Source:
Plant biotechnology journal 2020 v.18 no.11 pp. 2316-2327
ISSN:
1467-7644
Subject:
Arabidopsis; antibodies; arabinose; arabinoxylan; biotechnology; cell walls; crosslinking; dietary fiber; dimerization; endosperm; epitopes; gene expression regulation; hexaploidy; loss-of-function mutation; mutants; viscosity; wheat; xylan
Abstract:
... Wheat contains abundant xylan in cell walls of all tissues, but in endosperm, there is an unusual form of xylan substituted only by arabinose (arabinoxylan; AX) that has long chains and low levels of feruloylation, a fraction of which is extractable in water (WE‐AX). WE‐AX acts as soluble dietary fibre but also gives rise to viscous extracts from grain, a detrimental trait for some non‐food uses o ...
DOI:
10.1111/pbi.13393

35. Molecular Characterization, Regioselective and Synergistic Action of First Recombinant Type III α-L-arabinofuranosidase of Family 43 Glycoside Hydrolase (PsGH43_12) from Pseudopedobacter saltans

Author:
Abhijeet Thakur; Kedar Sharma; Sumitha Banu Jamaldheen; Arun Goyal
Source:
Molecular biotechnology 2020 v.62 no.9 pp. 443-455
ISSN:
1073-6085
Subject:
Bacteroides ovatus; Clostridium thermocellum; arabinose; arabinoxylan; biomass; biotechnology; calcium; glycosides; hydrolysis; moieties; molecular weight; pH; regioselectivity; rye; saccharification; synergism; temperature; wheat; xylanases; xylooligosaccharides; xylose
Abstract:
... α-L-Arabinofuranosidase (PsGH43_12) of family 43 glycoside hydrolase and subfamily 12 from Pseudopedobacter saltans was cloned, over-expressed and biochemically characterized. PsGH43_12 displayed molecular mass, ~ 65 kDa. It exhibited activity in pH (5–9) and temperature range (35–55 °C) with maxima at pH 6.5 and 50 °C. PsGH43_12 gave 88.7 U/mg specific activity against rye arabinoxylan and 78.9 U ...
DOI:
10.1007/s12033-020-00263-x

36. Recent advances in properties, production, and applications of L-ribulose

Author:
Jiajun Chen; Hao Wu; Wenli Zhang; Wanmeng Mu
Source:
Applied microbiology and biotechnology 2020 v.104 no.13 pp. 5663-5672
ISSN:
0175-7598
Subject:
arabinose; biochemical pathways; biotechnology; biotransformation; isomers; microbiology; ribulose; synthesis
Abstract:
... Currently, due to the special functions and potential application values, rare sugars become the hot topic in carbohydrate fields. L-Ribulose, an isomer of L-ribose, is an expensive rare ketopentose. As an important precursor for other rare sugars and L-nucleoside analogue synthesis, L-ribulose attracts more and more attention in recent days. Compared with complicated chemical synthesis, the bioco ...
DOI:
10.1007/s00253-020-10637-5

37. Study of the chemical structure of exopolysaccharide produced from streptomycete and its effect as an attenuate for antineoplastic drug 5-fluorouracil that induced gastrointestinal toxicity in rats

Author:
Manal G. Mahmoud; Manal S. Selim; Sahar S. Mohamed; Amal I. Hassan; Samia A. Abdal-Aziz
Source:
Animal biotechnology 2020 v.31 no.5 pp. 397-412
ISSN:
1532-2378
Subject:
antioxidant activity; apoptosis; arabinose; biotechnology; drug therapy; ethanol; exopolysaccharides; fluorouracil; galactose; glucose; high performance liquid chromatography; histopathology; intestines; intraperitoneal injection; males; mannose; molecular weight; neutrophils; nucleotide sequences; oxidative stress; sediments; sulfates; toxicity; uronic acids; villi; Egypt; Mediterranean Sea
Abstract:
... Chemotherapeutic medications, including 5 – fluorouracil (5FU), are the same old technique to most cancers and are associated with numerous peripheral toxicities. We investigated exopolysaccharide (EPSST) produced from the isolated streptomycete of the Mediterranean Sea for the capability to lower the severity of mucositis in vivo. The streptomycete was isolated from Mediterranean Sea sediment fro ...
DOI:
10.1080/10495398.2019.1610416

38. Valorisation of pectin-rich agro-industrial residues by yeasts: potential and challenges

Author:
Luís C. Martins; Catarina C. Monteiro; Paula M. Semedo; Isabel Sá-Correia
Source:
Applied microbiology and biotechnology 2020 v.104 no.15 pp. 6527-6547
ISSN:
0175-7598
Subject:
Saccharomyces cerevisiae; arabinose; biorefining; biotechnology; biotransformation; carbon; catabolism; feedstocks; galacturonic acid; value added; yeasts
Abstract:
... Pectin-rich agro-industrial residues are feedstocks with potential for sustainable biorefineries. They are generated in high amounts worldwide from the industrial processing of fruits and vegetables. The challenges posed to the industrial implementation of efficient bioprocesses are however manyfold and thoroughly discussed in this review paper, mainly at the biological level. The most important y ...
DOI:
10.1007/s00253-020-10697-7

39. Variation in sugarcane biomass composition and enzymatic saccharification of leaves, internodes and roots

Author:
Patrick J. Mason; Agnelo Furtado; Annelie Marquardt; Katrina Hodgson-Kratky; Nam V. Hoang; Frederik C. Botha; Gabriella Papa; Jenny C. Mortimer; Blake Simmons; Robert J. Henry
Source:
Biotechnology for biofuels 2020 v.13 no.1 pp. 201
ISSN:
1754-6834
Subject:
arabinose; biofuels; biomass; biorefining; biotechnology; carbon; cell walls; cellulose; culms; feedstocks; galactose; genotype; hemicellulose; internodes; leaves; lignin; p-coumaric acid; reducing sugars; roots; saccharification; storage; sucrose; sugarcane; tissues
Abstract:
... BACKGROUND: The composition of biomass determines its suitability for different applications within a biorefinery system. The proportion of the major biomass fractions (sugar, cellulose, hemicellulose and lignin) may vary in different sugarcane genotypes and growth environments and different parts of the plant. This study investigated the composition of mature and immature internodes, roots and ma ...
DOI:
10.1186/s13068-020-01837-2

40. Inhibitory effects of a sulfated polysaccharide isolated from edible red alga Bangia fusco-purpurea on α-amylase and α-glucosidase

Author:
Zedong Jiang; Gang Yu; Yan Liang; Tianyuan Song; Yanbing Zhu; Hui Ni; Kenichi Yamaguchi; Tatsuya Oda
Source:
Bioscience, biotechnology, and biochemistry 2019 v.83 no.11 pp. 2065-2074
ISSN:
1347-6947
Subject:
Bangia; Fourier transform infrared spectroscopy; alpha-amylase; arabinose; biotechnology; circular dichroism spectroscopy; fluorescence; fucose; galactose; gel chromatography; glucose; glucuronic acid; high performance liquid chromatography; mannose; molecular weight; polysaccharides; thin layer chromatography; xylose
Abstract:
... In this study, a sulfated polysaccharide (BFP) was isolated from the edible red alga Bangia fusco-purpurea. Gel-filtration and thin layer chromatographically analyses suggested that BFP was a homogenous polysaccharide. The chemical structural analysis revealed that BFP mainly consisted of galactose together with a small amount of uronic acid, mannose, and glucose. Its molecular mass was estimated ...
DOI:
10.1080/09168451.2019.1634515

41. Purification, structural elucidation and in vivo immunity-enhancing activity of polysaccharides from quinoa (Chenopodium quinoa Willd.) seeds

Author:
Sanhong Fan; Jiani Li; Baoqing Bai
Source:
Bioscience, biotechnology, and biochemistry 2019 v.83 no.12 pp. 2334-2344
ISSN:
1347-6947
Subject:
Chenopodium quinoa; arabinose; biotechnology; blood serum; cellulose; chemical structure; galactose; galacturonic acid; gel chromatography; glucose; hypersensitivity; immunosuppression; interleukin-6; lysozyme; macrophages; mannose; molecular weight; rhamnose; xylose
Abstract:
... Quinoa crude polysaccharides (QPS) were extracted from Chenopodium quinoa Willd. The soluble non-starch polysaccharide fraction (QPS1) was subsequently purified by DEAE-52 cellulose and Sephadex G-50 gel chromatography, using QPS as raw materials. Its chemical structure was identified using FT-IR, NMR, AFM, SEM and Congo red staining. High performance gel permeation chromatography (HPGPC) was used ...
DOI:
10.1080/09168451.2019.1650635

42. Identification and characterization of GH62 bacterial α-l-arabinofuranosidase from thermotolerant Streptomyces sp. SWU10 that preferentially degrades branched l-arabinofuranoses in wheat arabinoxylan

Author:
Pornpimol Phuengmaung; Yuika Kunishige; Wasana Sukhumsirichart; Tatsuji Sakamoto
Source:
Enzyme and microbial technology 2018 v.112 pp. 22-28
ISSN:
0141-0229
Subject:
EDTA (chelating agent); Escherichia coli; Streptomyces; alpha-N-arabinofuranosidase; amino acids; arabinans; arabinose; arabinoxylan; barium; biotechnology; calcium; copper; endo-1,4-beta-xylanase; enzyme activity; gene overexpression; glycosides; heat tolerance; hydrolysis; iron; manganese; molecular cloning; molecular weight; nuclear magnetic resonance spectroscopy; pH; signal peptide; silver; sugar beet; wheat; xylan; xylose
Abstract:
... We previously described thermotolerant Streptomyces sp. SWU10, which produced four endo-xylanases and one xylosidase able to digest xylan backbones. To achieve arabinoxylan degradation, the swu62A gene was cloned and overexpressed in Escherichia coli, and the recombinant enzyme, termed SWUAbf62A, was characterized. The 438 amino acids of SWUAbf62A revealed Glyco_hydro_62 and closely related with p ...
DOI:
10.1016/j.enzmictec.2018.01.009

43. Characterization and gene cloning of l-xylulose reductase involved in l-arabinose catabolism from the pentose-fermenting fungus Rhizomucor pusillus

Author:
Shino Yamasaki-Yashiki; Hidenobu Komeda; Kazuhiro Hoshino; Yasuhisa Asano
Source:
Bioscience, biotechnology, and biochemistry 2017 v.81 no.8 pp. 1612-1618
ISSN:
1347-6947
Subject:
Escherichia coli; L-xylulose reductase; Rhizomucor pusillus; acyl carrier protein; amino acid sequences; amino acids; arabinose; biotechnology; catabolism; fungi; genes; glucose; mannitol; xylitol; xylose
Abstract:
... l-Xylulose reductase (LXR) catalyzes the reduction of l-xylulose to xylitol in the fungal l-arabinose catabolic pathway. LXR (RpLXR) was purified from the pentose-fermenting zygomycetous fungus Rhizomucor pusillus NBRC 4578. The native RpLXR is a homotetramer composed of 29 kDa subunits and preferred NADPH as a coenzyme. The Kₘ values were 8.71 mM for l-xylulose and 3.89 mM for dihydroxyacetone. T ...
DOI:
10.1080/09168451.2017.1320518

44. Microwave-assisted extraction and pharmacological evaluation of polysaccharides from Posidonia oceanica

Author:
Yosra Ben Salem; Amal Abdelhamid; Khaoula Mkadmini Hammi; Didier Le Cerf; Abderrahman Bouraoui; Hatem Majdoub
Source:
Bioscience, biotechnology, and biochemistry 2017 v.81 no.10 pp. 1917-1925
ISSN:
1347-6947
Subject:
Posidonia oceanica; analgesic effect; animals; anti-inflammatory activity; arabinose; biotechnology; edema; galactose; gas chromatography-mass spectrometry; glucose; microwave treatment; molecular weight; polysaccharides; response surface methodology
Abstract:
... Microwave-assisted extraction was employed for the isolation of polysaccharides from Posidonia oceanica (PPO). The extracting parameters were optimized adopting response surface methodology. The highest polysaccharide yield (2.55 ± 0.09%), which is in concordance with the predicted value (2.76%), was obtained under the following conditions: extraction time 60 s, liquid–solid ratio of 50:1 (mL/g) a ...
DOI:
10.1080/09168451.2017.1361808

45. Molecular characterization of a glycoside hydrolase family-51 α-L-arabinofuranosidase from Auricularia auricula

Author:
Li Zou; Tingting Sun; Yuanyi Yang; Linfang Zhang; Jian Zhang
Source:
Biotechnology, biotechnological equipment 2017 v.31 no.3 pp. 469-476
ISSN:
1314-3530
Subject:
Auricularia auricula; Escherichia coli; arabinose; biotechnology; carbon; edible fungi; equipment; genes; glucose; glycosides; isoelectric point; maltose; molecular weight; mycelium; polyacrylamide gel electrophoresis; recombinant proteins; sodium dodecyl sulfate; sucrose
Abstract:
... The cDNA encoding α-L-arabinofuranosidase was cloned from the edible fungus Auricularia auricula for the first time. The open reading frame of the α-L-arabinofuranosidase gene abf was 1953 bp encoding 650 amino acids, with a predicted protein molecular weight of 71.19 kDa and a theoretical isoelectric point of 5.23. The putative protein was predicted to belong to the glycoside hydrolase family-51. ...
DOI:
10.1080/13102818.2017.1286233

46. Identification and characterization of d-arabinose reductase and d-arabinose transporters from Pichia stipitis

Author:
Seiya Watanabe; Yuki Utsumi; Shigeki Sawayama; Yasuo Watanabe
Source:
Bioscience, biotechnology, and biochemistry 2016 v.80 no.11 pp. 2151-2158
ISSN:
1347-6947
Subject:
Saccharomyces cerevisiae; Scheffersomyces stipitis; arabinose; biotechnology; carbon; enzyme activity; fungi; glucose; lignocellulose; oxidoreductases; pentoses; xylose
Abstract:
... d-xylose and l-arabinose are the major constituents of plant lignocelluloses, and the related fungal metabolic pathways have been extensively examined. Although Pichia stipitis CBS 6054 grows using d-arabinose as the sole carbon source, the hypothetical pathway has not yet been clarified at the molecular level. We herein purified NAD(P)H-dependent d-arabinose reductase from cells grown on d-arabin ...
DOI:
10.1080/09168451.2016.1204221

47. Structural characterization of sulfated arabinans extracted from Cladophora glomerata Kützing and their macrophage activation

Author:
Utoomporn Surayot; Ju Hun Lee; Chartchai Kanongnuch; Yuwadee Peerapornpisal; WooJung Park; SangGuan You
Source:
Bioscience, biotechnology, and biochemistry 2016 v.80 no.5 pp. 972-982
ISSN:
1347-6947
Subject:
Cladophora glomerata; arabinans; arabinose; biotechnology; cytokines; galactose; gel chromatography; glucose; ion exchange chromatography; macrophage activation; molecular weight; nitric oxide; rhamnose; water solubility; xylose
Abstract:
... Water-soluble sulfated heteropolysaccharides were extracted from Cladophora glomerata Kützing and fractionated by ion-exchange chromatography, which yielded two subfractions, F₁ and F₂. The crude and fractionated polysaccharides (F₁ and F₂) mostly consisted of carbohydrates (62.8–74.5%) with various amounts of proteins (9.00–17.3%) and sulfates (16.5–23.5%), including different levels of arabinose ...
DOI:
10.1080/09168451.2015.1132149

48. Antibacterial and Hemolytic Activity of a new Lectin Purified from the Seeds of Sterculia Foetida L.

Author:
Alana Araújo Braga; Rodrigo Rodrigues e Lacerda; Gracy Kelly Vieira de Vasconcelos Medeiros; Gregório Fernandes Gonçalves; Hilzeth de Luna Freire Pessoa; Juscélio Donizete Cardoso; Carlos Alberto de Almeida Gadelha; Bagnólia Araújo da Silva; Tatiane Santi-Gadelha
Source:
Applied biochemistry and biotechnology 2015 v.175 no.3 pp. 1689-1699
ISSN:
0273-2289
Subject:
EDTA (chelating agent); Sterculia; antibacterial properties; arabinose; biotechnology; carbohydrate content; cations; chelation; erythrocytes; glycoproteins; hemagglutination; hemolysis; high performance liquid chromatography; humans; ion exchange chromatography; lectins; microbial growth; molecular weight; octoxynol; oxidation; pH; polyacrylamide gel electrophoresis; seeds; sodium; temperature; urea; xylose
Abstract:
... The aim of this study was to isolate, characterize, and verify possible antibacterial and hemolytic activity for a lectin found in the seeds of Sterculia foetida L. Purification of the lectin from S. foetida (SFL) was realized with ion exchange chromatography DEAE-Sephacel coupled to HPLC. The purity and the molecular weight was determined by SDS-PAGE. The isolated SFL was characterized as to its ...
DOI:
10.1007/s12010-014-1390-4

49. Beneficial Effect of Corncob Acid Hydrolysate on the Lipid Production by Oleaginous Yeast Trichosporon dermatis

Author:
Lian Xiong; Chao Huang; Xiao-Yan Yang; Xiao-Qing Lin; Xue-Fang Chen; Can Wang; Bo Wang; Xin-An Zeng; Xin-De Chen
Source:
Preparative biochemistry & biotechnology 2015 v.45 no.5 pp. 421-429
ISSN:
1532-2297
Subject:
Trichosporon; acetic acid; arabinose; biomass; biotechnology; carbon; corn cobs; fermentation; glucose; hydrolysates; lignocellulose; lipid content; lipids; xylose; yeasts
Abstract:
... In this work, corncob acid hydrolysate and its simulated medium whose sugar composition was the same as the corncob acid hydrolysate were used as fermentation substrate for lipid production by oleaginous yeast Trichosporon dermatis . On the corncob acid hydrolysate, after 7 days of fermentation, the biomass, lipid content, lipid yield, and lipid coefficient of T. dermatis were 17.3 g/L, 40.2%, 7.0 ...
DOI:
10.1080/10826068.2014.923453

50. Biosynthesis and production of glycosylated flavonoids in Escherichia coli: current state and perspectives

Author:
Bong Gyu Kim; So Mi Yang; So Yeon Kim; Mi Na Cha; Joong-Hoon Ahn
Source:
Applied microbiology and biotechnology 2015 v.99 no.7 pp. 2979-2988
ISSN:
0175-7598
Subject:
Escherichia coli; arabinose; bacteria; biosynthesis; biotechnology; flavonoids; galactose; glucose; glucuronic acid; glycosylation; glycosyltransferases; methylation; rhamnose; secondary metabolites; uridine; xylose
Abstract:
... Flavonoids are plant secondary metabolites containing several hydroxyl groups that are targets for modification reactions such as methylation and glycosylation. In plants, flavonoids are present as glycones. Although glucose is the most common sugar attached to flavonoids, arabinose, galactose, glucuronic acid, rhamnose, and xylose are also linked to flavonoids. Depending on the kind and the posit ...
DOI:
10.1007/s00253-015-6504-6