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- Zhang, Bing-Zhu, et al. Show all 4 Authors
- Journal of agricultural and food chemistry 2010 v.58 no.5 pp. 2795-2800
- statistical models; phenylalanine ammonia-lyase; recombinant DNA; Escherichia coli; continuous systems; phenylalanine; enzyme activity; biotechnology; biotransformation
- ... Some approaches for improving recombinant phenylalanine ammonia lyase (PAL) stability in Escherichia coli during the enzymatic methods of l-phenylalanine (l-Phe) production were developed following preliminary studies by means of statistical-based experiment designs (response surface method). The traditional non-statistical technology was used to screen four critical factors for PAL stability duri ...