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1. A competitive colorimetric aptasensor for simple and sensitive detection of kanamycin based on terminal deoxynucleotidyl transferase-mediated signal amplification strategy

Author:
Tingting Zhao; Qian Chen; Yanli Wen; Xiaojun Bian; Qing Tao; Gang Liu; Juan Yan
Source:
Food chemistry 2022 v.377 pp. 132072
ISSN:
0308-8146
Subject:
aptasensors; biotin; color; colorimetry; detection limit; food chemistry; honey; kanamycin; magnetic separation; magnetism; milk; nucleotidyltransferases; oligonucleotides; peroxidase; streptavidin
Abstract:
... We developed a rapid and sensitive colorimetric biosensor based on competitive recognition between kanamycin (KAN), magnetic beads-kanamycin (MBs-KAN) and aptamer and terminal deoxynucleotidyl transferase (TdT)-mediated signal amplification strategy. In the absence of KAN, aptamers recognize MBs-KAN. TdT can amplify oligonucleotides to the 3′-OH ends of aptamers, with biotin-dUTP being embedded in ...
DOI:
10.1016/j.foodchem.2022.132072

2. A low-noise ratiometric fluorescence biosensor for detection of Pb2+ based on DNAzyme and exonuclease III–assisted cascade signal amplification

Author:
Huali Jin; Ruike Liu; Tian Bai; Min Wei; Baoshan He; Zhiguang Suo
Source:
Analytical and bioanalytical chemistry 2022 v.414 no.5 pp. 1899-1907
ISSN:
1618-2642
Subject:
analytical chemistry; biosensors; biotin; detection limit; enzymes; fluorescence; fluorescent dyes; heavy metals; humans; lead; magnetism; rice flour; single-stranded DNA; streptavidin; tap water; tea; toxicity
Abstract:
... As a common heavy metal ion with strong toxicity and wide distribution, lead ions (Pb²⁺) had great harm to the human body. In this work, a low-noise ratiometric fluorescence biosensor was developed based on Pb²⁺-dependent DNAzyme and exonuclease III (Exo III)–assisted cascade signal amplification. Firstly, the substrate chain of DNAzyme (S-DNA) was modified on the surface of magnetic beads (MBs) t ...
DOI:
10.1007/s00216-021-03825-3

3. A rapid RNA extraction-free lateral flow assay for molecular point-of-care detection of SARS-CoV-2 augmented by chemical probes

Author:
Ketan Dighe; Parikshit Moitra; Maha Alafeef; Nivetha Gunaseelan; Dipanjan Pan
Source:
Biosensors & bioelectronics 2022 v.200 pp. 113900
ISSN:
0956-5663
Subject:
COVID-19 infection; RNA; Severe acute respiratory syndrome coronavirus 2; biosensors; biotin; cysteamine; nanogold; oligonucleotides; pandemic; patients; point-of-care systems; reverse transcriptase polymerase chain reaction
Abstract:
... The coronavirus disease 2019 (COVID-19) pandemic has highlighted the major shortcoming of healthcare systems globally in their inability to diagnose the disease rapidly and accurately. At present, the molecular approaches for diagnosing COVID-19 primarily use reverse transcriptase polymerase chain reaction (RT-PCR) to create and amplify cDNA from severe acute respiratory syndrome coronavirus 2 (SA ...
DOI:
10.1016/j.bios.2021.113900

4. A simple method for labeling proteins and antibodies with biotin using the proximity biotinylation enzyme TurboID

Author:
Ryouhei Shioya; Kohdai Yamada; Kohki Kido; Hirotaka Takahashi; Akira Nozawa; Hidetaka Kosako; Tatsuya Sawasaki
Source:
Biochemical and biophysical research communications 2022 v.592 pp. 54-59
ISSN:
0006-291X
Subject:
Escherichia coli; biotin; biotinylation; green fluorescent protein; immunoblotting; research
Abstract:
... Proteins and antibodies labeled with biotin have been widely used for protein analysis, enzyme immunoassays, and diagnoses. Presently, they are prepared using either a chemical reaction involving a biotin N-hydroxysuccinimide (NHS) ester compound or by enzymatic biotin ligation using a combination of a biotinylation-peptide tag and Escherichia coli BirA. However, these methods are relatively compl ...
DOI:
10.1016/j.bbrc.2021.12.109

5. An ultrasensitive electrochemical immunosensor for hepatitis C antibodies based on one-step-eletrosynthetized polypyrrole–graphene nanocomposite

Author:
Gilvânia M. Santana; Anne K. S. Silva; Marcos V. Foguel; Rosa F. Dutra
Source:
Journal of materials science 2022 v.57 no.9 pp. 5586-5595
ISSN:
0022-2461
Subject:
antigens; biotin; blood serum; detection limit; electrochemistry; electrodes; ferricyanides; graphene; hepatitis C; immunosensors; nanocomposites; people; voltammetry
Abstract:
... An ultrasensitive label-free electrochemical immunosensor was developed for hepatitis C antibodies (anti-HCV). Worldwide, it is estimated 71 million people have HCV infection in a chronic stage that may lead to cirrose and cancer. To achieve HCV elimination, health programs should include screening testing based on anti-HCV detection allowing the early-stage treatment. The immunosensor was based o ...
DOI:
10.1007/s10853-022-06992-5

6. Antifouling peptides combined with recognizing DNA probes for ultralow fouling electrochemical detection of cancer biomarkers in human bodily fluids

Author:
Min Chen; Zhen Song; Xiqin Yang; Zhiling Song; Xiliang Luo
Source:
Biosensors & bioelectronics 2022 v.206 pp. 114162
ISSN:
0956-5663
Subject:
DNA; DNA probes; azides; biomarkers; biosensors; biotin; blood serum; detection limit; electrochemistry; electrodes; electroplating; humans; nanogold; oligonucleotides; peptides; streptavidin; sweat; urine
Abstract:
... Herein, a universal strategy for the construction of highly sensitive and low fouling biosensors was proposed based on antifouling peptides conjugated with recognizing DNA probes. The peptide-DNA conjugate was formed through a reagent-free click reaction between a typical DNA aptamer modified with 5′-dibenzocyclooctyne (DBCO) and the designed antifouling peptide terminated with biotin and the azid ...
DOI:
10.1016/j.bios.2022.114162

7. Artificial cell membrane camouflaged immunomagnetic nanoparticles for enhanced circulating tumor cell isolation

Author:
Xiaoxi Zhou; Yujia Zhang; Ke Kang; Nanhang Zhu; Jia Cheng; Qiangying Yi; Yao Wu
Source:
Journal of materials chemistry B 2022 v.10 no.16 pp. 3119-3125
ISSN:
2050-7518
Subject:
absorption; adsorption; antibodies; biomimetics; biotin; camouflage; cell membranes; commercialization; graphene; human serum albumin; magnetism; models; nanosheets; neoplasm cells; phospholipids
Abstract:
... Precise and specific circulating tumor cell (CTC) isolation is heavily interfered with by blood cells and proteins. Although satisfactory results have been achieved by some cell membrane-derived platforms, the following limitations have seriously limited the commercialization potential: complex membrane composition, difficult batch difference control, inconvenient source cell expansion, etc. To ov ...
DOI:
10.1039/d1tb02676c

8. Biorecognition Layer Based On Biotin-Containing [1]Benzothieno[3,2-b][1]benzothiophene Derivative for Biosensing by Electrolyte-Gated Organic Field-Effect Transistors

Author:
Elena Yu. Poimanova; Polina A. Shaposhnik; Daniil S. Anisimov; Elena G. Zavyalova; Askold A. Trul; Maxim S. Skorotetcky; Oleg V. Borshchev; Dmitry Z. Vinnitskiy; Marina S. Polinskaya; Vadim B. Krylov; Nikolay E. Nifantiev; Elena V. Agina; Sergey A. Ponomarenko
Source:
ACS applied materials & interfaces 2022 v.14 no.14 pp. 16462-16476
ISSN:
1944-8252
Subject:
Influenza A virus; biosensors; biotin; chemical species; lab-on-a-chip technology; point-of-care systems; sodium chloride; transistors
Abstract:
... Requirements of speed and simplicity in testing stimulate the development of modern biosensors. Electrolyte-gated organic field-effect transistors (EGOFETs) are a promising platform for ultrasensitive, fast, and reliable detection of biological molecules for low-cost, point-of-care bioelectronic sensing. Biosensitivity of the EGOFET devices can be achieved by modification with receptors of one of ...
DOI:
10.1021/acsami.1c24109

9. Biotin-streptavidin sandwich integrated PDA-ZnO@Au nanocomposite based SPR sensor for hIgG detection

Author:
Haohua Yang; Xueqi Zhao; Ziwei Zhang; Pinyi Ma; Xinghua Wang; Daqian Song; Ying Sun
Source:
Talanta 2022 v.246 pp. 123496
ISSN:
0039-9140
Subject:
biocompatibility; biosensors; biotin; enzymes; fluorescence; gold; humans; nanocomposites; nanocrystals; streptavidin; surface area; zinc oxide
Abstract:
... SPR is a mature optical biosensor technology for detecting biomolecular interactions without fluorescence or enzyme labeling. In this paper, we acquire a sensitive SPR biosensor based on ZnO@Au nanomaterial, and the classical sandwich strategy using biotin-streptavidin for secondary signal amplification system was used to detect human IgG (hIgG). Nano-zinc oxide (ZnO) has the dual characteristics ...
DOI:
10.1016/j.talanta.2022.123496

10. CeO2/MXene heterojunction-based ultrasensitive electrochemiluminescence biosensing for BCR-ABL fusion gene detection combined with dual-toehold strand displacement reaction for signal amplification

Author:
Wenqian Cheng; Zi Lin; Lina Zhao; Ningke Fan; Huijie Bai; Wei Cheng; Min Zhao; Shijia Ding
Source:
Biosensors & bioelectronics 2022 v.210 pp. 114287
ISSN:
0956-5663
Subject:
biosensors; biotin; detection limit; electrochemiluminescence; electrodes; genes; hot water treatment; leukemia; nanosheets; streptavidin
Abstract:
... An “on-off” nonenzymatic and ultrasensitive electrochemiluminescence (ECL) biosensing platform has been constructed to detect BCR-ABL fusion gene based on CeO₂/MXene heterojunction and configuration-entropy driven dual-toehold strand displacement reaction (DT-SDR) for signal amplification. The CeO₂/MXene heterojunction were prepared via one-step hydrothermal method through in situ synthesis of CeO ...
DOI:
10.1016/j.bios.2022.114287

11. Comparison of gut microbiota compositions and corresponding genetic and metabolic features between guttate and plaque psoriasis by metagenomic sequencing

Author:
Xiaoxu Wang; Xin Liu; Shiju Xiao; Zongfeng Zhang; Lingjun Wu; Yungchi Cheng; Yong Tan; Guangzhong Zhang; Chunyan Jiang
Source:
Microbial pathogenesis 2022 pp. 105560
ISSN:
0882-4010
Subject:
Human gammaherpesvirus 4; Megamonas; biosynthesis; biotin; butylamine; coagulation; complement; folic acid; glutamic acid; glutamine; insulin resistance; intestinal microorganisms; metabolites; metagenomics; pathogenesis; proteoglycans; psoriasis; ribosomes; secretion; sphingolipids; steroid hormones; thiamin
Abstract:
... Guttate psoriasis (GP) and psoriasis plaques (PP) are common subtypes of psoriasis. Previous studies have fully researched the association between psoriasis and gut microbiota. However, the differences in gut microbiota between GPs and PPs are still unknown. Fecal samples were collected from 30 psoriatic patients (15 GP and 15 PP) and 15 healthy subjects. Metagenomic sequencing was then used to co ...
DOI:
10.1016/j.micpath.2022.105560

12. Coupled synthetic pathways improve the production of 3-hydroxypropionic acid in recombinant Escherichia coli strains

Author:
Dafeng Zhou; Diego Leandro Quiroga-Sánchez; Xuan Zhang; Yanhong Chang; Hui Luo
Source:
Biotechnology notes 2022 v.3 pp. 25-31
ISSN:
2665-9069
Subject:
Candida rugosa; Chloroflexus aurantiacus; Escherichia coli; Ustilago zeae; acetyl-CoA carboxylase; bicarbonates; biotechnology; biotin; glucose; liquid state fermentation; malonyl coenzyme A; oxidoreductases
Abstract:
... To improve the production of 3-HP with glucose as a substrate, the malonyl-CoA and propionyl-CoA pathways were coupled to regulate NADP⁺/NADPH regeneration in the recombinant E. coli. The strain Ec-AM that overexpressed the key enzymes of the malonyl-CoA pathway, acetyl CoA carboxylase (ACC) from Ustilago maydis and malonyl CoA reductase (MCR) from Chloroflexus aurantiacus, produced 0.26 g/L of 3- ...
DOI:
10.1016/j.biotno.2022.02.002

13. Crystal structure of the Cys-NO modified YopH tyrosine phosphatase

Author:
Ruth F. Rocha; Priscila G.A. Martins; Humberto D'Muniz Pereira; José Brandão-Neto; Otavio Henrique Thiemann; Hernán Terenzi; Angela C.O. Menegatti
Source:
Biochimica et biophysica acta 2022 v.1870 no.3 pp. 140754
ISSN:
1570-9639
Subject:
S-nitrosylation; X-ray diffraction; Yersinia enterocolitica; antibiotic resistance; biotin; catalytic activity; circular dichroism spectroscopy; crystal structure; cysteine; enzyme activity; mass spectrometry; mutants; nitric oxide; pathogens; protein structure; protein-tyrosine-phosphatase; serine; signal transduction; therapeutics; tyrosine; virulence
Abstract:
... Protein tyrosine phosphatases (PTPs) are key virulence factors in pathogenic bacteria, consequently, they have become important targets for new approaches against these pathogens, especially in the fight against antibiotic resistance. Among these targets of interest YopH (Yersinia outer protein H) from virulent species of Yersinia is an example. PTPs can be reversibly inhibited by nitric oxide (NO ...
DOI:
10.1016/j.bbapap.2022.140754

14. Development of Fc-specific multi-biotinylated antibodies via photoreactive tandem AviTag repeats for the ultrasensitive determination of ochratoxin A

Author:
Xiao-Yi Gao; Chong-Mei Xu; Xiao-Kun Zhang; Meng-Ran Li; Xiao-Ming Gong; Hong-Ming Yang; Jin-Bao Tang
Source:
Food control 2022 v.132 pp. 108525
ISSN:
0956-7135
Subject:
aminoacyl tRNA ligases; biotin; chemical bonding; detection limit; enzyme-linked immunosorbent assay; food contamination; ochratoxin A
Abstract:
... Ochratoxin A (OTA) is a common food contaminant that seriously threatens the safety and health of consumers. Here, an indirect competitive biotin-streptavidin-amplified enzyme-linked immunosorbent assay (icBA-ELISA) was developed for the sensitive detection of OTA by employing Fc-specific multi-biotinylated antibodies. An Fc-binding protein (Z-domain) carrying a photo-cross-linker (p-benzoylphenyl ...
DOI:
10.1016/j.foodcont.2021.108525

15. Development of a Highly Sensitive Biotin-Streptavidin Amplified Enzyme-Linked Immunosorbent Assay for Determination of Progesterone in Milk Samples

Author:
Minglei Lu; Minting Liang; Junkang Pan; Yingying Zhong; Chunguo Zhang; Xiping Cui; Tiantian Wang; Jinfeng Yan; Jinlong Ding; Suqing Zhao
Source:
Food analytical methods 2022 v.15 no.2 pp. 541-551
ISSN:
1936-9751
Subject:
antibodies; biotin; detection limit; enzyme-linked immunosorbent assay; milk; progesterone; public health; streptavidin; tandem mass spectrometry
Abstract:
... Agricultural product contamination by endocrine-disrupting compounds (EDCs) residues is an international public health issue and requires continuously stringent administration. A high-throughput biotin-streptavidin-amplified enzyme-linked immunosorbent assay (BA-ELISA) for detection of progesterone (P4) has been established to be applied in determining milk samples. Under experimental optimization ...
DOI:
10.1007/s12161-021-02137-7

16. Effect of Coix Seed Extracts on Growth and Metabolism of Limosilactobacillus reuteri

Author:
Zhoujie Yang; Anyan Wen; Likang Qin; Yi Zhu
Source:
Foods 2022 v.11 no.2 pp. -
ISSN:
2304-8158
Subject:
Coix lacryma-jobi; Oriental traditional medicine; acetic acid; amino acid metabolism; biosynthesis; biotin; carbohydrate metabolism; lactic acid; metabolites; metabolomics; pharmacology; polysaccharides; prebiotics; seed extracts; seed oils
Abstract:
... Coix seed (Coix lachryma-jobi L.) is an important nourishing food and traditional Chinese medicine. The role of their bioactive constituents in physiology and pharmacology has received considerable scientific attention. However, very little is known about the role of coix seed bioactive components in the growth of Limosilactobacillus reuteri (L. reuteri). This study aimed to evaluate the effects o ...
DOI:
10.3390/foods11020187

17. Glucose and Ethanol Detection with an Affinity-Switchable Lateral Flow Assay

Author:
Hsiao-Jung Huang; Yu-Ting Lin; Min-Chi Chung; Yu-Hsuan Chen; Kui-Thong Tan
Source:
Analytical chemistry 2022 v.94 no.12 pp. 5084-5090
ISSN:
1520-6882
Subject:
alcohol oxidase; analytical chemistry; antibodies; antigens; biotin; blood serum; disease prevention; ethanol; glucose; glucose oxidase; gold; hydrogen peroxide; infectious diseases; pregnancy; streptavidin; urine
Abstract:
... The lateral flow assay (LFA) is one of the most successful analytical platforms for rapid on-site detection of target substances. This type of assay has been used in many rapid diagnoses, for example, pregnancy tests and infectious disease prevention. However, applications of LFAs for very small molecules remain a demanding challenge due to the problem of obtaining the corresponding binding partne ...
DOI:
10.1021/acs.analchem.1c05316

18. Highly-sensitive and simple fluorescent aptasensor for 17 b-estradiol detection coupled with HCR-HRP structure

Author:
Hao Yang; Danke Xu
Source:
Talanta 2022 v.240 pp. 123094
ISSN:
0039-9140
Subject:
aptasensors; biotin; catalytic activity; fluorescence; humans; hybridization chain reaction; magnetism; microparticles; milk; oligonucleotides; peroxidase; polystyrenes; pregnancy; society
Abstract:
... As an important kind of environmental endocrine disruptors, 17 β -Estradiol (E2) plays a major role in affecting the growth of human including sexual characters, pregnancy system, etc. In the modern society, with the threat of abuse in breeding, it is imperative to design sensitive methods for detecting low concentration of E2 in environment. In this work, we constructed a highly sensitive and sim ...
DOI:
10.1016/j.talanta.2021.123094

19. Microfluidics-assisted synthesis and functionalization of monodisperse colloidal hydrogel particles for optomechanical biosensors

Author:
David Rettke; Christian Danneberg; Talika Alina Neuendorf; Sebastian Kühn; Jens Friedrichs; Nicolas Hauck; Carsten Werner; Julian Thiele; Tilo Pompe
Source:
Journal of materials chemistry B 2022 v.10 no.10 pp. 1663-1674
ISSN:
2050-7518
Subject:
biosensors; biotin; energy; exposure duration; hydrogels; microfluidic technology; microscopy; molecular weight; polyethylene glycol; spectroscopy; thiols
Abstract:
... The soft colloidal probe (SCP) assay is a highly versatile sensing principle employing micrometer-sized hydrogel particles as optomechanical transducer elements. We report the synthesis, optimization, and conjugation of SCPs with defined narrow size distribution and specifically tailored mechanical properties and functionalities for integration into a microinterferometric optomechanical biosensor ...
DOI:
10.1039/d1tb02798k

20. Plasmonically-enhanced competitive assay for ultrasensitive and multiplexed detection of small molecules

Author:
Zheyu Wang; Qingjun Zhou; Anushree Seth; Samhitha Kolla; Jingyi Luan; Qisheng Jiang; Priya Rathi; Prashant Gupta; Jeremiah J. Morrissey; Rajesh R. Naik; Srikanth Singamaneni
Source:
Biosensors & bioelectronics 2022 v.200 pp. 113918
ISSN:
0956-5663
Subject:
biomedical research; biosensors; biotin; bovine serum albumin; chemical species; cortisol; fluorescein; fluorescence; fluorescence microscopy; food safety; gold; metabolites; molecular weight; nanorods; transmission electron microscopy; ultraviolet-visible spectroscopy
Abstract:
... Novel methods that enable facile, ultrasensitive and multiplexed detection of low molecular weight organic compounds such as metabolites, drugs, additives, and organic pollutants are valuable in biomedical research, clinical diagnosis, food safety and environmental monitoring. Here, we demonstrate a simple, rapid, and ultrasensitive method for detection and quantification of small molecules by imp ...
DOI:
10.1016/j.bios.2021.113918