U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Subject term biotin Remove constraint Subject term: biotin
Start Over

Search

2,153  Search Results

« Previous | 1 - 50 of 2,153 |
Number of results to display per page

Search Results

1. 12-Plex DiLeu Isobaric Labeling Enabled High-Throughput Investigation of Citrullination Alterations in the DNA Damage Response

Author:
Zihui Li; Bin Wang; Qinying Yu; Yatao Shi; Lingjun Li
Source:
Analytical chemistry 2022 v.94 no.7 pp. 3074-3081
ISSN:
1520-6882
Subject:
DNA damage; DNA repair; analytical chemistry; arginine; biotin; humans; leucine; mass spectrometry; pathogenesis; post-translational modification; quantitative analysis; thiols
Abstract:
... Protein citrullination is a key post-translational modification (PTM) that leads to the loss of positive charge on arginine and consequent protein structural and functional changes. Though it has been indicated to play critical roles in various physiological and pathological processes, effective analytical tools are largely limited due to a few challenges such as the small mass shift induced by th ...
DOI:
10.1021/acs.analchem.1c04073

2. 1H NMR-based metabolomic study of the effects of flavonoids on citrinin production by Monascus

Author:
Shanshan He; Yanling Wang; Jianhua Xie; Heng Gao; Xiujiang Li; Zhibing Huang
Source:
Food research international 2020 v.137 pp. 109532
ISSN:
0963-9969
Subject:
Monascus; alanine; apigenin; arginine; aspartic acid; biosynthesis; biotin; citrinin; daidzein; food research; genistein; glutamic acid; glutathione; kaempferol; lysine; metabolomics; proline; secondary metabolites
Abstract:
... Monascus comprises purple-red molds. Various compounds can be obtained from these species, including statins and food-safe yellow, red, and orange pigments. However, the secondary metabolite citrinin, a mycotoxin, is produced during the late stages of growth. Citrinin biosynthesis should be reduced to apply Monascus pigments safely. Fortunately, this can be achieved by the addition of flavonoids ( ...
DOI:
10.1016/j.foodres.2020.109532

3. 2′-Fluoro ribonucleic acid modified DNA dual-probe sensing strategy for enzyme-amplified electrochemical detection of double-strand DNA of PML/RARα related fusion gene

Author:
Yun Lei; Kun Wang; Shan-Yue Wu; Dan-Dan Huang; Ming Dai; Yan-Jie Zheng; Zhou-Liang Sun; Yuan-Zhong Chen; Xin-Hua Lin; Ai-Lin Liu
Source:
Biosensors & bioelectronics 2018 v.112 pp. 170-176
ISSN:
0956-5663
Subject:
DNA; RNA; biosensors; biotin; denaturation; electrochemistry; electrodes; streptavidin
Abstract:
... In the study, a novel sensing strategy based on dual-probe mode, which involved two groups of 2′-fluoro ribonucleic acid (2′-F RNA) modified probes, was designed for the detection of synthetic target double-strand DNA (dsDNA) of PML/RARα fusion genes in APL. And each pair of probes contained a thiolated capture probe (C1 or C2) immobilized on one of electrode surfaces in the dual-channel electroch ...
DOI:
10.1016/j.bios.2018.04.012

4. 3D printing with peptide–polymer conjugates for single-step fabrication of spatially functionalized scaffolds

Author:
Paula Camacho; Hafiz Busari; Kelly B. Seims; Peter Schwarzenberg; Hannah L. Dailey; Lesley W. Chow
Source:
Biomaterials science 2019 v.7 no.10 pp. 4237-4247
ISSN:
2047-4849
Subject:
adhesion; azides; biocompatible materials; biodegradability; biotin; cell adhesion; fibroblasts; fluorescent dyes; peptides; polymers; printers; three-dimensional printing
Abstract:
... Biodegradable polymer-based scaffolds are widely used to provide support during early stages of regeneration and can be functionalized with various chemical groups or bioactive cues to promote desired cellular behavior. However, these scaffolds are often modified post-fabrication, which can lead to undesired changes and homogeneously distributed chemistries that fail to mimic the spatial biochemic ...
DOI:
10.1039/c9bm00887j

5. 3D-nanostructured scaffold electrodes based on single-walled carbon nanotubes and nanodiamonds for high performance biosensors

Author:
Meenakshi Singh; Michael Holzinger; Olga Biloivan; Serge Cosnier
Source:
Carbon 2013 v.61 no. pp. 349-356
ISSN:
0008-6223
Subject:
biosensors; biotin; carbon; carbon nanotubes; coatings; electrodes; glucose oxidase; nanodiamonds
Abstract:
... We report a novel strategy for controlled nanostructuring of electrodes surfaces using single-walled nanotubes and nanodiamonds in order to form highly porous structures for biosensors applications. These nanostructures were obtained by subsequent deposition of nanotubes and nanodiamonds followed by functionalization of this assembly with pyrenenitrilotriacteic acid (NTA) via dip coating. The elec ...
DOI:
10.1016/j.carbon.2013.05.015

6. 55 Peri-conceptional undernourishment perturbs offspring sperm methylome

Author:
P. Toschi; E. Capra; D. Anzalone; F. Turri; F. Pizzi; B. Lazzari; A. Stella; P. Ajmone-Marsan; P. Loi
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 153
ISSN:
1031-3613
Subject:
DNA methylation; adults; animal models; biotin; bisulfites; blastocyst; body weight; chondroitin sulfate; chromatin; conception; diet; embryogenesis; epigenetics; flow cytometry; folic acid; gene ontology; genotype; glucagon; inheritance (genetics); ion transport; lambs; malnutrition; maternal exposure; maternal nutrition; metabolism; mothers; nutrient availability; phenotype; potassium; progeny; rams; semen; sequence analysis; sperm motility; spermatozoa
Abstract:
... Whereas an organism’s genotype is relatively static throughout life, the epigenome is highly dynamic and can adapt, or be altered, in response to the external environment such as diet. Intrauterine exposure to nutrient availability can alter the establishment of epigenetic marks, not only in the exposed individuals, but also in their offspring. Inheritance of such an environmentally acquired pheno ...
DOI:
10.1071/RDv31n1Ab55

7. 5′-Terminal chemical capping of spliced leader RNAs

Author:
Karolina Piecyk; Richard E. Davis; Marzena Jankowska-Anyszka
Source:
Tetrahedron letters 2012 v.53 no.36 pp. 4843-4847
ISSN:
0040-4039
Subject:
Nematoda; biotin; chemical reactions; chemical structure; messenger RNA; mutants; nucleotides; organic compounds; trans-splicing; translation (genetics)
Abstract:
... Spliced leader (SL) RNA trans-splicing adds a N²,N²,7-trimethylguanosine cap (TMG) and a 22-nucleotide sequence, the SL, to the 5′ end of mRNAs. Both non-trans-spliced with a monomethylguanosine cap (MMG) and trans-spliced mRNAs co-exist in trans-splicing metazoan cells. Efficient translation of TMG-capped mRNAs in nematodes requires a defined core of nucleotides within the SL sequence. Here we pr ...
DOI:
10.1016/j.tetlet.2012.06.127
PubMed:
23175583
PubMed Central:
PMC3501006

8. A Bifunctional Locus (BIO3-BIO1) Required for Biotin Biosynthesis in Arabidopsis

Author:
Muralla, Rosanna; Chen, Elve; Sweeney, Colleen; Gray, Jennifer A.; Dickerman, Allan; Nikolau, Basil J.; Meinke, David
Source:
Plant physiology 2008 v.146 no.1 pp. 60-73
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; Escherichia coli; auxotrophs; bacteria; biochemical pathways; biosynthesis; biotin; enzymatic reactions; enzymes; genetic complementation; genetic techniques and protocols; loci; operon; phenotype; proteins
Abstract:
... We identify here the Arabidopsis (Arabidopsis thaliana) gene encoding the third enzyme in the biotin biosynthetic pathway, dethiobiotin synthetase (BIO3; At5g57600). This gene is positioned immediately upstream of BIO1, which is known to be associated with the second reaction in the pathway. Reverse genetic analysis demonstrates that bio3 insertion mutants have a similar phenotype to the bio1 and ...
DOI:
10.1104/pp.107.107409
PubMed:
17993549
PubMed Central:
PMC2230573

9. A Biotin Switch-Based Proteomics Approach Identifies 14-3-3ζ as a Target of Sirt1 in the Metabolic Regulation of Caspase-2

Author:
Joshua L. Andersen; J. Will Thompson; Kelly R. Lindblom; Erika S. Johnson; Chih-Sheng Yang; Lauren R. Lilley; Christopher D. Freel; M. Arthur Moseley; Sally Kornbluth
Source:
Molecular cell 2011 v.43 no.5 pp. 834-842
ISSN:
1097-2765
Subject:
Xenopus; acetylation; apoptosis; biotin; caspase-2; cultured cells; death; dissociation; eggs; glucose 6-phosphate; humans; lysine; proteomics
Abstract:
... While lysine acetylation in the nucleus is well characterized, comparatively little is known about its significance in cytoplasmic signaling. Here we show that inhibition of the Sirt1 deacetylase, which is primarily cytoplasmic in cancer cell lines, sensitizes these cells to caspase-2-dependent death. To identify relevant Sirt1 substrates, we developed a proteomics strategy, enabling the identific ...
DOI:
10.1016/j.molcel.2011.07.028
PubMed:
21884983
PubMed Central:
PMC3417139

10. A Collagen Supplement Improves Skin Hydration, Elasticity, Roughness, and Density: Results of a Randomized, Placebo-Controlled, Blind Study

Author:
Liane Bolke; Gerrit Schlippe; Joachim Gerß; Werner Voss
Source:
Nutrients 2019 v.11 no.10 pp. -
ISSN:
2072-6643
Subject:
acerolas; ascorbic acid; biotin; collagen; dietary supplements; drinking; fruit extracts; health services; peptides; placebos; roughness; silicon; skin (animal); ultrasonography; vitamin E; women; zinc; Germany
Abstract:
... The purpose of this randomized, placebo-controlled, blind study was to investigate the effects of the drinkable nutraceutical ELASTEN® (QUIRIS Healthcare, Gütersloh, Germany) on skin aging and skin health. Drinking ampoules provides a blend of 2.5 g of collagen peptides, acerola fruit extract, vitamin C, zinc, biotin, and a native vitamin E complex. This controlled interventional trial wa ...
DOI:
10.3390/nu11102494
PubMed:
31627309
PubMed Central:
PMC6835901

11. A Comparative Study on Date Syrup (Dips) as Substrate for the Production of Baker’s Yeast (Saccharomyces cerevisiae)

Author:
Al-Jasass, F.M.; Al-Eid, S.M.; Ali, S.H.H.
Source:
Acta horticulturae 2010 v. no.882 pp. 699-704
ISSN:
0567-7572
Subject:
Saccharomyces cerevisiae; biotin; butyric acid; formic acid; magnesium; molasses; nitrogen content; nutrient content; pantothenic acid; phosphorus; potassium; propionic acid; sugars; toxicity; yeasts
Abstract:
... The suitability of date syrup (Dips) as a substrate for the production of baker’s yeast (Saccharomyces cerevisiae) was examined and compared with molasses substrate as a reference. With regard to nutrient content, Dips compares very well with molasses in this respect. Dips contains more sugars, biotin and pantothenic acid than molasses, about similar contents of nitrogen, phosphorus and magnesium, ...

12. A Comparison of Vitamin and Lutein Concentrations in Breast Milk from Four Asian Countries

Author:
My Tuyen Thi Nguyen; Jieun Kim; Hyunjun Lee; Soyoon Won; Yongki Kim; Ji A. Jung; Dan Li; Xuan Hong Mai To; Khanh Trang Nguyen Huynh; Thanh Van Le; Beenish Israr; Hyun Joo An; Jaehan Kim
Source:
Nutrients 2020 v.12 no.6 pp. -
ISSN:
2072-6643
Subject:
biotin; breast milk; folic acid; humans; information; lutein; milk; nutritional status; pantothenic acid; pyridoxine; riboflavin; thiamin; vitamin A; vitamin A deficiency; China; Korean Peninsula; Pakistan; Vietnam
Abstract:
... Vitamins are the essential elements for human life and, particularly, for infant health. Human milk is the best source of nutrients for newborns, however, the information of vitamins in Asian maternal milk is still limited. In this study, we have collected 580 Asian maternal milk samples from Korea (n = 254), China (n = 137), Pakistan (n = 92), and Vietnam (n = 97). The vitamin concentrations, inc ...
DOI:
10.3390/nu12061794

13. A Corynebacterium glutamicum gene encoding a two-domain protein similar to biotin carboxylases and biotin-carboxyl-carrier proteins

Author:
Jager, W.; Peters-Wendisch, P.G.; Kalinowski, J.; Puhler, A.
Source:
Archives of microbiology 1996 v.166 no.2 pp. 76-82
ISSN:
0302-8933
Subject:
Corynebacterium glutamicum; bacterial proteins; structural genes; biotin; carboxy-lyases; binding proteins; acetyl-CoA carboxylase; DNA; amino acid sequences; sequence alignment
Abstract:
... Following the analysis of transposon Tn5432-induced mutants of Corynebacterium glutamicum ATCC 13032, a gene encoding a protein with a biotin-binding motif was cloned. The DNA sequence of this gene revealed an open reading frame encoding 591 amino acids with a calculated mol. mass of 63.4 kDa. The protein is composed of two domains, an N-terminal biotin carboxylase and a C-terminal biotin-carboxyl ...
DOI:
10.1007/s002030050359
PubMed:
8772169

14. A DNA-Mediated Homogeneous Binding Assay for Proteins and Small Molecules

Author:
Zhang Zhao; Hejesen Christian; Kjelstrup Michael B.; Birkedal Victoria; Gothelf Kurt V.
Source:
Journal of the American Chemical Society 2014 v.136 no.31 pp. 11115-11120
ISSN:
1520-5126
Subject:
antibodies; biotin; digoxigenin; folic acid; oligonucleotides; proteins; vitamin D
Abstract:
... Optical detection of molecular targets typically requires immobilization, separation, or chemical or enzymatic processing. An important exception is aptamers that allow optical detection in solution based on conformational changes. This method, however, requires the laborious selection of aptamers with high target specificity and affinity, and the ability to undergo the required conformational cha ...
DOI:
10.1021/ja505519b

15. A DNAzyme assay coupled with effective magnetic separation and rolling circle amplification for detection of lead cations with a smartphone camera

Author:
Wei Lu; Caiqin Lin; Jun Yang; Xiaoqi Wang; Bo Yao; Min Wang
Source:
Analytical and bioanalytical chemistry 2019 v.411 no.21 pp. 5383-5391
ISSN:
1618-2642
Subject:
biotin; cameras; cations; ecotoxicology; fluorescence; health services; humans; instrumentation; lakes; lead; magnetic separation; magnetism; mobile telephones; monitoring
Abstract:
... Rapid, portable, and efficient detection of lead cations (Pb²⁺) is of great significance for monitoring and evaluating environmental toxicity and human healthcare. In this work, we developed a simple and low-cost homogenous fluorescence DNAzyme assay for Pb²⁺ determination based on Pb²⁺-dependent cleaving and rolling circle amplification (RCA). DNAzyme and its substrate (5′-biotin) formed double-s ...
DOI:
10.1007/s00216-019-01879-y

16. A Dermonutrient Containing Special Collagen Peptides Improves Skin Structure and Function: A Randomized, Placebo-Controlled, Triple-Blind Trial Using Confocal Laser Scanning Microscopy on the Cosmetic Effects and Tolerance of a Drinkable Collagen Supplement

Author:
Sabrina Laing; Stephan Bielfeldt; Carolin Ehrenberg; Klaus-Peter Wilhelm
Source:
Journal of medicinal food 2020 v.23 no.2 pp. 147-152
ISSN:
1557-7600
Subject:
acerolas; ascorbic acid; biotin; collagen; dietary supplements; females; fruit extracts; health status; image analysis; oral exposure; peptides; randomized clinical trials; skin (animal); vitamin E; volunteers; zinc
Abstract:
... The purpose of this randomized, placebo-controlled, triple-blind trial on 60 healthy female volunteers was to assess the cosmetic effects on skin quality of a food supplement containing special collagen peptides together with acerola extract, vitamin C, vitamin E, biotin, and zinc after an intake of 12 weeks (Elasten®, QUIRIS Healthcare, Germany). To reduce assessment bias maximally and increase t ...
DOI:
10.1089/jmf.2019.0197
PubMed:
32017646
PubMed Central:
PMC7041324

17. A Dual-Targeting Delivery System for Effective Genome Editing and In Situ Detecting Related Protein Expression in Edited Cells

Author:
Bo-Ya Liu; Xiao-Yan He; Chang Xu; Lei Xu; Shu-Lun Ai; Si-Xue Cheng; Ren-Xi Zhuo
Source:
Biomacromolecules 2018 v.19 no.7 pp. 2957-2968
ISSN:
1526-4602
Subject:
biotin; calcium carbonate; calcium phosphates; cell nucleus; chitosan; coprecipitation; electrostatic interactions; gene editing; gene expression; ligands; messenger RNA; nanoparticles; neoplasm cells; organelles; plasmids; polymers; pro-apoptotic proteins; protein synthesis; proteins; sulfates; vascular endothelial growth factors
Abstract:
... One of critical steps in genome editing by CRISPR-Cas9 is to deliver the CRISPR-Cas9 system into targeted cells. In this study, we developed a dual-targeting delivery system based on polymer/inorganic hybrid nanoparticles to realize highly efficient genome editing in targeted tumor cells as well as in situ detection on the related protein expression in edited cells. The CRISPR-Cas9 plasmid for CDK ...
DOI:
10.1021/acs.biomac.8b00511

18. A G‐quadruplex/hemin DNAzyme‐based microchip electrophoresis chemiluminescence assay for highly sensitive detection of biotin in flour

Author:
Jian Li; Shuting Li; Jingjin Zhao; Yong Huang; Shulin Zhao
Source:
Electrophoresis 2019 v.40 no.16-17 pp. 2157-2164
ISSN:
0173-0835
Subject:
biotin; catalytic activity; chemiluminescence; detection limit; electrophoresis; flour; hydrogen peroxide; microchip technology; quantitative analysis; redox reactions; streptavidin
Abstract:
... Quantitative analysis of biotin in biological fluids, foods, and pharmaceutical is important for diagnosis and treatment of biotin‐related diseases and health maintenance. In this work, a novel G‐quadruplex/hemin DNAzyme‐based microchip electrophoresis chemiluminescence (CL) assay method was established for rapid and highly sensitive detection of biotin. This method is based on the specific bindin ...
DOI:
10.1002/elps.201800535

19. A High-Sensitivity Lanthanide Nanoparticle Reporter for Mass Cytometry: Tests on Microgels as a Proxy for Cells

Author:
Lin Wanjuan; Hou Yi; Lu Yijie; Abdelrahman Ahmed I.; Cao Pengpeng; Zhao Guangyao; Tong Lemuel; Qian Jieshu; Baranov Vladimir; Nitz Mark; Winnik Mitchell A.
Source:
Langmuir 2014 v.30 no.11 pp. 3142-3153
ISSN:
1520-5827
Subject:
adsorption; biomarkers; biotin; biotinylation; bovine serum albumin; chemical bonding; ions; models; nanoparticles; polymers; streptavidin
Abstract:
... This paper addresses the question of whether one can use lanthanide nanoparticles (e.g., NaHoF₄) to detect surface biomarkers expressed at low levels by mass cytometry. To avoid many of the complications of experiments on live or fixed cells, we carried out proof-of-concept experiments using aqueous microgels with a diameter on the order of 700 nm as a proxy for cells. These microgels were used to ...
DOI:
10.1021/la403627p
PubMed:
24617504
PubMed Central:
PMC3983384

20. A Label-Free Potentiometric Sensor Principle for the Detection of Antibody–Antigen Interactions

Author:
Ozdemir Mahir S.; Marczak Marcin; Bohets Hugo; Bonroy Kristien; Roymans Dirk; Stuyver Lieven; Vanhoutte Koen; Pawlak Marcin; Bakker Eric
Source:
Analytical chemistry 2013 v.85 no.9 pp. 4770-4776
ISSN:
1520-6882
Subject:
ammonium compounds; antibodies; antigen-antibody reactions; bioassays; biotin; chemistry; poly(vinyl chloride); quartz; streptavidin; viruses
Abstract:
... We report here on a new potentiometric biosensing principle for the detection of antibody–antigen interactions at the sensing membrane surface without the need to add a label or a reporter ion to the sample solution. This is accomplished by establishing a steady-state outward flux of a marker ion from the membrane into the contacting solution. The immunobinding event at the sensing surface retards ...
DOI:
10.1021/ac400514u

21. A Large Solvent Isotope Effect on Protein Association Thermodynamics

Author:
Eginton Christopher; Beckett Dorothy
Source:
Biochemistry 2013 v.52 no.38 pp. 6595-6600
ISSN:
1520-4995
Subject:
DNA; Gibbs free energy; biotin; deuterium oxide; dimerization; energy; isotopes; protein-protein interactions; solvents; temperature
Abstract:
... Solvent reorganization can contribute significantly to the energetics of protein–protein interactions. However, our knowledge of the magnitude of the energetic contribution is limited, in part, by a dearth of quantitative experimental measurements. The biotin repressor forms a homodimer as a prerequisite to DNA binding to repress transcription initiation. At 20 °C, the dimerization reaction, which ...
DOI:
10.1021/bi400952m
PubMed:
23984950
PubMed Central:
PMC3919658

22. A New Method To Determine In Vivo Interactomes Reveals Binding of the Legionella pneumophila Effector PieE to Multiple Rab GTPases

Author:
Aurélie Mousnier; Gunnar N. Schroeder; Charlotte A. Stoneham; Ernest C. So; James A. Garnett; Lu Yu; Steve J. Matthews; Jyoti S. Choudhary; Elizabeth L. Hartland; Gad Frankel
Source:
mBio 2014 v.5 no.4 pp. e01148-14
ISSN:
2150-7511
Subject:
Escherichia coli; Legionella pneumophila; affinity chromatography; biotin; biotinylation; endoplasmic reticulum; guanosinetriphosphatase; mass spectrometry; nickel; nitrilotriacetic acid; pathogens; purification methods; streptavidin; two hybrid system techniques; type IV secretion system; vacuoles
Abstract:
... Legionella pneumophila , the causative agent of Legionnaires’ disease, uses the Dot/Icm type IV secretion system (T4SS) to translocate more than 300 effectors into host cells, where they subvert host cell signaling. The function and host cell targets of most effectors remain unknown. PieE is a 69-kDa Dot/Icm effector containing three coiled-coil (CC) regions and 2 transmembrane (TM) helices follow ...
DOI:
10.1128/mBio.01148-14
PubMed:
25118235
PubMed Central:
PMC4145681

23. A Novel Class of ER Membrane Proteins Regulates ER-Associated Endosome Fission

Author:
Melissa J. Hoyer; Patrick J. Chitwood; Christopher C. Ebmeier; Jonathan F. Striepen; Robert Z. Qi; William M. Old; Gia K. Voeltz
Source:
Cell 2018 v.175 no.1 pp. 254-265.e14
ISSN:
0092-8674
Subject:
actin; biotin; buds; endoplasmic reticulum; endosomes; membrane proteins
Abstract:
... Endoplasmic reticulum (ER) membrane contact sites (MCSs) mark positions where endosomes undergo fission for cargo sorting. To define the role of ER at this unique MCS, we targeted a promiscuous biotin ligase to cargo-sorting domains on endosome buds. This strategy identified the ER membrane protein TMCC1, a member of a conserved protein family. TMCC1 concentrates at the ER-endosome MCSs that are s ...
DOI:
10.1016/j.cell.2018.08.030
PubMed:
30220460
PubMed Central:
PMC6195207

24. A Novel Method to Construct Dual-targeted Magnetic Nanoprobes by Modular Assembling

Author:
Chen Bai; Pengcheng Hu; Di Liu; Yi Chen; Ming Ma; Ning Gu; Yu Zhang
Source:
Colloids and surfaces 2020 v.605 pp. 125339
ISSN:
0927-7757
Subject:
T-lymphocytes; biotin; cytotoxicity; hydrodynamics; immunotherapy; iron oxides; ligands; magnetism; streptavidin
Abstract:
... Magnetic nanoparticle is an important branch of nanomaterials, especially magnetic iron oxide (Fe₃O₄) nanoparticles have attracted widespread attention due to the fact that they can be used not only as high-sensitivity magnetic resonance contrast agents, but also as carriers for the construction of multifunctional and intelligent nanoprobes. The functional ligands modified on the surface of nanopr ...
DOI:
10.1016/j.colsurfa.2020.125339

25. A Nucleic Acid Nanostructure Built through On-Electrode Ligation for Electrochemical Detection of a Broad Range of Analytes

Author:
Subramaniam Somasundaram; Christopher J. Easley
Source:
Journal of the American Chemical Society 2019 v.141 no.29 pp. 11721-11726
ISSN:
1520-5126
Subject:
DNA; biomarkers; biotin; blood serum; catalytic activity; digoxigenin; drugs; electrochemistry; electrodes; humans; nanomaterials; organ transplantation; patients; point-of-care systems; streptavidin; tacrolimus
Abstract:
... For an assay to be most effective in point-of-care clinical analysis, it needs to be economical, simple, generalizable, and free from tedious workflows. While electrochemistry-based DNA sensors reduce instrumental costs and eliminate complicated procedures, there remains a need to address probe costs and generalizability, as numerous probes with multiple conjugations are needed to quantify a wide ...
DOI:
10.1021/jacs.9b06229
PubMed:
31257869
PubMed Central:
PMC6800171

26. A Programmable DNA Double-Write Material: Synergy of Photolithography and Self-Assembly Nanofabrication

Author:
Song Youngjun; Takahashi Tsukasa; Kim Sejung; Heaney Yvonne C.; Warner John; Chen Shaochen; Heller Michael J.
Source:
ACS Applied Materials & Interfaces 2017 v.9 no.1 pp. 22-28
ISSN:
1944-8252
Subject:
biotin; complementary DNA; fluorescence; nucleotide sequences; streptavidin
Abstract:
... We demonstrate a DNA double-write process that uses UV to pattern a uniquely designed DNA write material, which produces two distinct binding identities for hybridizing two different complementary DNA sequences. The process requires no modification to the DNA by chemical reagents and allows programmed DNA self-assembly and further UV patterning in the UV exposed and nonexposed areas. Multilayered ...
DOI:
10.1021/acsami.6b11361

27. A Set of Polymorphic Trinucleotide and Tetranucleotide Microsatellite Markers for Silver Crucian Carp (Carassius auratus gibelio) and Cross-Amplification in Crucian Carp

Author:
Zheng, X. H.; Lu, C. Y.; Zhao, Y. Y.; Lee, C.; Cao, D. C.; Chang, Y. M.; Liang, L. Q.; Sun, X. W.
Source:
Biochemical genetics 2010 v.48 no.7-8 pp. 624-635
ISSN:
0006-2928
Subject:
Carassius auratus; Carassius gibelio; alleles; biotin; fish; genetic markers; gynogenesis; heterozygosity; loci; models; nucleic acid hybridization
Abstract:
... Silver crucian carp (Carassius auratus gibelio Bloch), as a gynogenetic fish, is a promising model for the study of the evolutionary genetics of vertebrates. We have developed 59 polymorphic trinucleotide and tetranucleotide markers for the silver crucian carp through the biotin capture method and radioactive-labeling hybridization. The number of alleles ranged from 2 to 12 in the population, and ...
DOI:
10.1007/s10528-010-9344-1
PubMed:
20503069

28. A Short- and Long-Range Fluorescence Resonance Energy Transfer-Cofunctionalized Fluorescence Quenching Collapsar Probe Regulates Amplified and Accelerated Detection of Salmonella

Author:
Xinlei Zhang; Jianguo Xu; Chao Yan; Li Yao; Huijie Shang; Wei Chen
Source:
Journal of agricultural and food chemistry 2021 v.69 no.47 pp. 14294-14301
ISSN:
1520-5118
Subject:
Salmonella; biotin; bulls; carbonated beverages; diagnostic techniques; energy transfer; fluorescence; food chemistry; food safety; green tea; human health; milk; orange juice; probability; reaction kinetics; safety assessment; signal-to-noise ratio; streptavidin; tap water
Abstract:
... Accurate and rapid quantification of foodborne pathogens is of great significance for food safety and human health. In this work, we have successfully constructed a fluorescence quenching collapsar probe (FQCP) on the basis of a conventional aptamer-encoded molecular beacon (AEMB) and applied it for the detection of Salmonella. In structure, the FQCP is assembled by AEMBs in fours via specific str ...
DOI:
10.1021/acs.jafc.1c05780

29. A Streptavidin Binding Site Mutation Yields an Unexpected Result: An Ionized Asp128 Residue Is Not Essential for Strong Biotin Binding

Author:
Baugh Loren; Le Trong Isolde; Stayton Patrick S.; Stenkamp Ronald E.; Lybrand Terry P.
Source:
Biochemistry 2016 v.55 no.37 pp. 5201-5203
ISSN:
1520-4995
Subject:
binding capacity; binding sites; biotin; ionization; point mutation; streptavidin
Abstract:
... We report a detailed study of a point mutation of the crucial binding site residue, D128, in the biotin–streptavidin complex. The conservative substitution, D128N, preserves the detailed structure observed for the wild-type complex but has an only minimal impact on biotin binding, even though previous experimental and computational studies suggested that a charged D128 residue was crucial for high ...
DOI:
10.1021/acs.biochem.6b00698
PubMed:
27603565
PubMed Central:
PMC5030189

30. A Surface Biotinylation Strategy for Reproducible Plasma Membrane Protein Purification and Tracking of Genetic and Drug-Induced Alterations

Author:
Hörmann Katrin; Stukalov Alexey; Müller André C.; Heinz Leonhard X.; Superti-Furga Giulio; Colinge Jacques; Bennett Keiryn L.
Source:
Journal of Proteome Research 2016 v.15 no.2 pp. 647-658
ISSN:
1535-3907
Subject:
biotin; biotinylation; coatings; databases; drug therapy; drugs; liquid chromatography; mass spectrometry; membrane proteins; plasma membrane; prediction; proteome; proteomics; silica; swine; tunicamycin
Abstract:
... Plasma membrane (PM) proteins contribute to the identity of a cell, mediate contact and communication, and account for more than two-thirds of known drug targets.1−8 In the past years, several protocols for the proteomic profiling of PM proteins have been described. Nevertheless, comparative analyses have mainly focused on different variations of one approach.9−11 We compared sulfo-NHS-SS-biotinyl ...
DOI:
10.1021/acs.jproteome.5b01066

31. A Synthetic Peptide-Based Assay System for Detecting Binding between CD36 and an Oxidized Low-Density Lipoprotein

Author:
Satoshi TSUZUKI; Marie TAKAI; Yukari MATSUNO; Yuki KOZAI; Maiko FUJIOKA; Kozue KAMEI; Hitomi INAGAKI; Ai EGUCHI; Shigenobu MATSUMURA; Kazuo INOUE; Tohru FUSHIKI
Source:
Bioscience, biotechnology, and biochemistry 2013 v.77 no.1 pp. 132-137
ISSN:
1347-6947
Subject:
amino acid sequences; amino acids; biotechnology; biotin; glycerophospholipids; low density lipoprotein; membrane proteins; mice; oxidation; peptides
Abstract:
... CD36 is an integral membrane protein that mediates the cellular uptake of oxidized low-density lipoprotein (oxLDL) through recognition of the oxidized glycerophospholipids (oxPLs) formed during LDL oxidation. We aimed to devise an assay system to detect binding between CD36 and oxLDL/oxPL without using recombinant proteins. A peptide corresponding to amino-acid residues 149–168 of mouse CD36 with ...
DOI:
10.1271/bbb.120625

32. A Synthetic Vesicle-to-Vesicle Communication System

Author:
Yudi Ding; Nicholas H. Williams; Christopher A. Hunter
Source:
Journal of the American Chemical Society 2019 v.141 no.44 pp. 17847-17853
ISSN:
1520-5126
Subject:
binding capacity; binding sites; biotin; catalytic activity; communications technology; dissociation; encapsulation; fluorescence; hydrolysis; ligands
Abstract:
... A molecular signal displayed on the external surface of one population of vesicles was used to trigger a catalytic process on the inside of a second population of vesicles. The key recognition event is the transfer of a protein (NeutrAvidin) bound to vesicles displaying desthiobiotin to vesicles displaying biotin. The desthiobiotin–protein complex was used to anchor a synthetic transducer in the o ...
DOI:
10.1021/jacs.9b09102

33. A T7 exonuclease assisted dual-cycle signal amplification assay of miRNA using nanospheres-enhanced fluorescence polarization

Author:
Xiaoting Li; Nian Huang; Liangliang Zhang; Jingjin Zhao; Shulin Zhao
Source:
Talanta 2019 v.202 pp. 297-302
ISSN:
0039-9140
Subject:
DNA fragmentation; biomedical research; biotin; enzymes; fluorescence; fluorescent dyes; microRNA; nanospheres; neoplasms; nucleic acid hybridization; streptavidin
Abstract:
... Based on streptavidin coated nanospheres and T7 exonuclease assisted dual-cycle signal amplification, we developed a novel sensitive fluorescence polarization detection method for miRNA. When target miRNA was present in the system, hairpin probe hybridized with miRNA, forming a double-stranded structure. The 5′ end of hairpin probe was then recognized and digested by T7 exonuclease, releasing the ...
DOI:
10.1016/j.talanta.2019.05.006

34. A Transcriptomic Analysis of Gene Expression in Chieh-Qua in Response to Fusaric Acid Stress

Author:
Min Wang; Wenrui Liu; Jinqiang Yan; Piaoyun Sun; Feng Chen; Biao Jiang; Dasen Xie; Yu’e Lin; Qingwu Peng; Xiaoming He
Source:
Horticulturae 2021 v.7 no.4 pp. -
ISSN:
2311-7524
Subject:
Benincasa hispida; Fusarium; Fusarium wilt; apoptosis; biosynthesis; biotin; cultivars; enzyme activity; ethylene; fusaric acid; gene expression; gene expression regulation; malondialdehyde; pathogenesis-related proteins; peroxidase; secondary metabolites; sequence analysis; superoxide dismutase; transcriptomics
Abstract:
... Fusarium wilt results in undesirable effects on the quality and production of chieh-qua (Benincasa hispida Cogn. var. Chieh-qua How). Fusaric acid (FA), a secondary metabolite of biotin produced by pathogens of genus Fusarium, induced resistant responses in chieh-qua; however, the physiological and molecular mechanism(s) of FA resistance remains largely unknown. In our study, ‘A39’ (FA-resistant c ...
DOI:
10.3390/horticulturae7040088

35. A Vector Design that Allows Fast and Convenient Production of Differently Tagged Proteins

Author:
Omar Scapolan; Andrea N. Mazzarello; Maria Bono; Marzia Occhino; Vasily Ogryzko; Marco Bestagno; Paolo Scartezzini; Silvia Bruno; Franco Fais; Fabio Ghiotto
Source:
Molecular biotechnology 2012 v.52 no.1 pp. 16-25
ISSN:
1073-6085
Subject:
DNA; biotin; introns; proteins
Abstract:
... Recombinant-tagged proteins have a widespread use in experimental research as well as in clinical diagnostic and therapeutic approaches. Well-stocked sets of differently tagged variants of a same protein would be of great help. However, the construction of differently tagging vectors is a demanding task since cloning procedures need several tailored DNA inserts. In this study, we describe a novel ...
DOI:
10.1007/s12033-011-9469-4
PubMed:
22076571

36. A Well-Controlled BioID Design for Endogenous Bait Proteins

Author:
Giel Vandemoortele; Delphine De Sutter; Aline Moliere; Jarne Pauwels; Kris Gevaert; Sven Eyckerman
Source:
Journal of proteome research 2018 v.18 no.1 pp. 95-106
ISSN:
1535-3907
Subject:
biotin; cell lines; clonal variation; clones; colorectal neoplasms; enzymes; genetic engineering; neoplasm cells; proteins; proteome; proteomics; risk; synthetic biology
Abstract:
... The CRISPR/Cas9 revolution is profoundly changing the way life sciences technologies are used. Many assays now rely on engineered clonal cell lines to eliminate the overexpression of bait proteins. Control cell lines are typically nonengineered cells or engineered clones, implying a considerable risk for artifacts because of clonal variation. Genome engineering can also transform BioID, a proximit ...
DOI:
10.1021/acs.jproteome.8b00367

37. A Y-Shaped Three-Arm Structure for Probing Bivalent Interactions between Protein Receptor–Ligand Using AFM and SPR

Author:
Subhadip Senapati; Sudipta Biswas; Saikat Manna; Robert Ros; Stuart Lindsay; Peiming Zhang
Source:
Langmuir 2018 v.34 no.23 pp. 6930-6940
ISSN:
1520-5827
Subject:
atomic force microscopy; biotin; crystal structure; ligands; models; moieties; streptavidin; surface plasmon resonance
Abstract:
... The goal of this research was to develop linkage chemistry for the study of bivalent interactions between a receptor and its ligand using atomic force microscopy (AFM) and surface plasmon resonance (SPR). We conceived a three-arm structure composed of flexible chains connected to a large rigid core with orthogonal functional groups at their ends for formation and attachment (or immobilization) of ...
DOI:
10.1021/acs.langmuir.8b00735

38. A biosensor based on a film bulk acoustic resonator and biotin–avidin system for the detection of the epithelial tumor marker mucin 1

Author:
Peng Guo; Juan Xiong; Dan Zheng; Weihai Zhang; Lei Liu; Shengfu Wang; Haoshuang Gu
Source:
RSC advances 2015 v.5 no.81 pp. 66355-66359
ISSN:
2046-2069
Subject:
acoustics; biosensors; biotin; epithelium; films (materials); mucins; neoplasms; streptavidin
Abstract:
... AlN thin film bulk acoustic resonators (FBARs) with a resonant frequency of ∼575 MHz have been fabricated to function as an epithelial tumor marker mucin 1 (MUC1) biosensor. Streptavidin was assembled on the sensitive area of FBAR. After the recognition between aptamers–AuNP conjugates and MUC1, biotin, along with the conjugates, was captured by streptavidin onto the surface of FBARs. Therefore, t ...
DOI:
10.1039/c5ra06016h

39. A biotin enrichment strategy identifies novel carbonylated amino acids in proteins from human plasma

Author:
Jesper F. Havelund; Katarzyna Wojdyla; Michael J. Davies; Ole N. Jensen; Ian Max Møller; Adelina Rogowska-Wrzesinska
Source:
Journal of proteomics 2017 v.156 pp. 40-51
ISSN:
1874-3919
Subject:
alanine; aldehydes; aspartic acid; avidin; biotin; bovine serum albumin; chromatography; glutamic acid; glutamine; humans; ions; isoleucine; leucine; lysine; methionine; oxidation; oxidative stress; peptides; protein structure; tandem mass spectrometry; valine
Abstract:
... Protein carbonylation is an irreversible protein oxidation correlated with oxidative stress, various diseases and ageing. Here we describe a peptide-centric approach for identification and characterisation of up to 14 different types of carbonylated amino acids in proteins. The modified residues are derivatised with biotin-hydrazide, enriched and characterised by tandem mass spectrometry. The stre ...
DOI:
10.1016/j.jprot.2016.12.019

40. A biotin receptor-targeted silicon(IV) phthalocyanine for in vivo tumor imaging and photodynamic therapy

Author:
Ke Li; Wenyi Dong; Qingzhu Liu; Gaochao Lv; Minhao Xie; Xinchen Sun; Ling Qiu; Jianguo Lin
Source:
Journal of photochemistry and photobiology 2019 v.190 pp. 1-7
ISSN:
1011-1344
Subject:
bioactive properties; biotin; fluorescence; human cell lines; hydrophilicity; image analysis; in vitro studies; irradiation; mice; models; moieties; neoplasms; photochemotherapy; photosensitizing agents; polyethylene glycol; silicon; tissue distribution; water solubility
Abstract:
... Phthalocyanines (Pcs) are a kind of potential photosensitizers for fluorescence imaging and photodynamic therapy (PDT). However, the clinical application of Pcs is suffered from their poor solubility, high aggregation tendency and low tumor-specificity. To address these problems, two biotin moieties were linked to the axial positions of silicon(IV) phthalocyanine (SiPc) through hydrophilic polyeth ...
DOI:
10.1016/j.jphotobiol.2018.09.001

41. A biotin-guided fluorescent probe for dual-mode imaging of viscosity in cancerous cells and tumor tissues

Author:
Mingguang Ren; Qingyu Xu; Shoujuan Wang; Lu Liu; Fangong Kong
Source:
Chemical communications 2020 v.56 no.87 pp. 13351-13354
ISSN:
1364-548X
Subject:
biotin; fluorescence; fluorescent dyes; neoplasm cells; neoplasms; viscosity
Abstract:
... A cancer cell targeted fluorescent viscosity probe has been designed and synthesized to specifically visualise viscosity changes in biotin receptor (BiR) positive cells over biotin negative cells via dual-mode fluorescence imaging: fluorescence intensity mode and fluorescence lifetime mode. ...
DOI:
10.1039/d0cc05039c

42. A biotin-guided fluorescent-peptide drug delivery system for cancer treatment

Author:
Taeyoung Kim; Hyun Mi Jeon; Hoa Thi Le; Tae Woo Kim; Chulhun Kang; Jong Seung Kim
Source:
Chemical communications 2014 v.50 no.57 pp. 7690-7693
ISSN:
1364-548X
Subject:
biotin; cell viability; chemical compounds; chemical reactions; drug delivery systems; fluorescence; human cell lines; neoplasms
Abstract:
... Herein, we present a fluorescent-peptide drug delivery system composed of biotin–naphthalimide–HJ inhibitor peptide2, prodrug 1. Treatment of 1 to biotin receptor-positive HepG2 cells, which are resistant to high concentrations of the HJ inhibitor peptide2, decreased cell viability and increased intracellular fluorescence. ...
DOI:
10.1039/c4cc02878c

43. A biotin-guided formaldehyde sensor selectively detecting endogenous concentrations in cancerous cells and tissues

Author:
Yun Hak LeeContributed equally.; Yonghe Tang; Peter Verwilst; Weiying Lin; Jong Seung Kim
Source:
Chemical communications 2016 v.52 no.75 pp. 11247-11250
ISSN:
1364-548X
Subject:
biotin; chemical reactions; formaldehyde; neoplasm cells; neoplasms; tissues
Abstract:
... A biotin appended formaldehyde sensor was found to specifically visualise both exogenous and endogenous levels of formaldehyde in biotin receptor positive cells over biotin negative cells by means of one- and two-photon excitation. The probe furthermore visualised endogenous levels of formaldehyde in tumour tissue slices up to 70 μm depth. ...
DOI:
10.1039/c6cc06158c

44. A biotin-guided hydrogen sulfide fluorescent probe and its application in living cell imaging

Author:
Chen Zhang; Jiewen Zhang; Zhiqiang Xu; Kun Zang; Feng Liu; Jun Yin; Ying Tan; Yuyang Jiang
Source:
RSC advances 2020 v.10 no.59 pp. 36135-36140
ISSN:
2046-2069
Subject:
biotin; detection; fluorescent dyes; hepatocytes; hydrogen sulfide; image analysis; liver neoplasms; metabolism; monitoring; neoplasm cells
Abstract:
... Hydrogen sulfide (H₂S), a well-known signaling molecule, exerts significant regulatory effects on the cardiovascular and nervous systems. Therefore, monitoring the metabolism of H₂S offers a potential mechanism to detect various diseases. In addition, biotin is significantly used as a targeting group to detect cancer cells exclusively. In this work, a biotin-guided benzoxadizole-based fluorescent ...
DOI:
10.1039/d0ra06524b

45. A biotin-regulated locus, bioS, in a possible survival operon of Rhizobium meliloti

Author:
Streit, W.R.; Phillips, D.A.
Source:
Molecular plant-microbe interactions 1997 v.10 no.7 pp. 933-937
ISSN:
0894-0282
Subject:
rhizosphere; loci; mutagenesis; Ensifer meliloti; amino acid sequences; chromosome mapping; bacterial proteins; reporter genes; biotin; phosphotransferases (kinases); operon; promoter regions; mortality
Abstract:
... A novel biotin-inducible DNA locus, bioS, was found in Rhizobium meliloti 1021 by mutation with a transposable promoter probe. The promoterless nptII reporter responded to 40 nM biotin with a sixfold increase in NPTII protein. Stationary-phase mutant cells accumulated 50% more 14C-biotin than corresponding wild-type cells. The prototrophic mutant competed equally with the wild type in biotin-free ...
DOI:
10.1094/MPMI.1997.10.7.933
PubMed:
9304864

46. A cancer cell-specific two-photon fluorescent probe for imaging hydrogen sulfide in living cells

Author:
Xuezhen Song; Baoli Dong; Xiuqi Kong; Chao Wang; Nan Zhang; Weiying Lin
Source:
RSC advances 2017 v.7 no.26 pp. 15817-15822
ISSN:
2046-2069
Subject:
azides; biotin; cell proliferation; fluorescence; fluorescent dyes; hydrogen sulfide; image analysis; monitoring; neoplasm cells; neoplasms
Abstract:
... Hydrogen sulfide (H₂S) could induce the proliferation of cancer cells in a concentration-dependent manner, and has close relation with the tumor growth. Monitoring the H₂S level in real-time is of great important for understanding its roles in the cancer cell proliferation and the diagnosis of the tumor. Herein, a novel cancer cell-specific two-photon fluorescent probe BN-H₂S for detecting H₂S in ...
DOI:
10.1039/c7ra01479a

47. A capillary electrophoretic assay for acetyl coenzyme A carboxylase

Author:
Sherrisse K. Bryant; Grover L. Waldrop; S. Douglass Gilman
Source:
Analytical biochemistry 2013 v.437 no.1 pp. 32-38
ISSN:
0003-2697
Subject:
acetyl coenzyme A; acetyl-CoA carboxylase; adenosine diphosphate; adenosine triphosphate; biotin; capillary electrophoresis; malonyl coenzyme A
Abstract:
... A simple off-column capillary electrophoretic (CE) assay for measuring acetyl coenzyme A carboxylase holoenzyme (holo-ACC) activity and inhibition was developed. The two reactions catalyzed by the holo-ACC components, biotin carboxylase (BC) and carboxyltransferase (CT), were simultaneously monitored in this assay. Acetyl coenzyme A (CoA), malonyl-CoA, adenosine triphosphate (ATP), and adenosine d ...
DOI:
10.1016/j.ab.2013.02.005
PubMed:
23435309
PubMed Central:
PMC3627828

48. A class of selective antibacterials derived from a protein kinase inhibitor pharmacophore

Author:
Miller, J. Richard; Dunham, Steve; Mochalkin, Igor; Banotai, Craig; Bowman, Matthew; Buist, Susan; Dunkle, Bill; Hanna, Debra; Harwood, H. James; Huband, Michael D.; Karnovsky, Alla; Kuhn, Michael; Limberakis, Chris; Liu, Jia Y.; Mehrens, Shawn; Mueller, W. Thomas; Narasimhan, Lakshmi; Ogden, Adam; Ohren, Jeff; Prasad, J.V.N. Vara; Shelly, John A.; Skerlos, Laura; Sulavik, Mark; Thomas, V. Hayden; VanderRoest, Steve; Wang, LiAnn; Wang, Zhigang; Whitton, Amy; Zhu, Tong; Stover, C. Kendall
Source:
Proceedings of the National Academy of Sciences of the United States of America 2009 v.106 no.6 pp. 1737-1742
ISSN:
0027-8424
Subject:
G-protein coupled receptors; Haemophilus influenzae; active sites; antibiotics; bacteria; biosynthesis; biotin; drug resistance; fastidious bacteria; fatty acids; genes; humans; mechanism of action; pathogens; pharmacology; protein kinases; screening
Abstract:
... As the need for novel antibiotic classes to combat bacterial drug resistance increases, the paucity of leads resulting from target-based antibacterial screening of pharmaceutical compound libraries is of major concern. One explanation for this lack of success is that antibacterial screening efforts have not leveraged the eukaryotic bias resulting from more extensive chemistry efforts targeting euk ...
DOI:
10.1073/pnas.0811275106
PubMed:
19164768
PubMed Central:
PMC2644107

49. A cleavable biotin tagging reagent that enables the enrichment and identification of carbonylation sites in proteins

Author:
Chelsea M. Coffey; Scott Gronert
Source:
Analytical and bioanalytical chemistry 2016 v.408 no.3 pp. 865-874
ISSN:
1618-2642
Subject:
acrolein; biotin; human serum albumin; liquid chromatography; proteomics; streptavidin; tandem mass spectrometry
Abstract:
... The utility of a new, cleavable tag for identifying and enriching protein carbonyls is examined. Using a model system, human serum albumin modified with acrolein, the EZ-Link alkoxyamine-PEG₄-SS-PEG₄-biotin affinity tag, was tested for its ability to label protein carbonyls in proteomic analyses of protein carbonylation. The efficiency of the labeling was assayed and compared to standard biotin hy ...
DOI:
10.1007/s00216-015-9176-2

50. A clickable glutamine (CliQ) derivative for the traceless reversible modification of peptides and proteins

Author:
Samuel D. Whedon; Marissa K. Parker; Elizabeth L. Tyson; Tobias Ritterhoff; Patrick M. M. Shelton; Champak Chatterjee
Source:
Chemical communications 2019 v.55 no.14 pp. 2043-2045
ISSN:
1364-548X
Subject:
biotin; chemical communication; chemical reactions; copper; glutamine; peptides; proteins
Abstract:
... The Cu(i)-mediated click reaction of proteins with affinity tags enables their selective isolation from complex mixtures. However, irreversible protein modification limits the interpretation of results from subsequent biophysical and biochemical assays. We report a facile and modular chemical strategy to reversibly modify peptides and proteins with biotin and FLAG affinity tags at a clickable glut ...
DOI:
10.1039/c8cc09404g
PubMed:
30687853
PubMed Central:
PMC6784325