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You searched for: Subject term blight Remove constraint Subject term: blight Subject term DNA primers Remove constraint Subject term: DNA primers
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1. Selection of wild Cicer accessions for the generation of mapping populations segregating for resistance to ascochyta blight

Author:
Collard, B.C.Y.; Pang, E.C.K.; Taylor, P.W.J.
Source:
Euphytica 2003 v.130 no.1 pp. 1-9
ISSN:
0014-2336
Subject:
random amplified polymorphic DNA technique; blight; wild relatives; fungal diseases of plants; DNA; genetic distance; Ascochyta rabiei; DNA primers; chickpeas; plant pathogenic fungi; disease resistance; chromosome mapping; genetic variation; population; Cicer arietinum; repetitive sequences; genetic markers

2. Development of a multiplex classical polymerase chain reaction technique for detection of Didymella bryoniae in infected cucumber tissues and greenhouse air samples

Author:
Koch, C.A.; Utkhede, R.S.
Source:
Canadian journal of plant pathology 2004 v.26 no.3 pp. 291-298
ISSN:
0706-0661
Subject:
blight; Cucumis sativus; fungal diseases of plants; Didymella bryoniae; cucumbers; polymerase chain reaction; DNA primers; pollutants; pathogen identification; Phoma; plant pathogenic fungi; nucleotide sequences; air pollution; greenhouses
Abstract:
... Two sets of primer pairs were evaluated for their usefulness in detecting and identifying Didymella bryoniae (anamorph Phoma cucurbitacearum), causal agent of gummy stem blight, in infected cucumber plant tissues and air samples, using polymerase chain reaction. One primer in each pair was a universal primer that has been used for amplification of septate fungi. The other primer in each pair was a ...
DOI:
10.1080/07060660409507146

3. Specific Detection of Xanthomonas axonopodis pv. dieffenbachiae in Anthurium (Anthurium andreanum) Tissues by Nested PCR

Author:
Robène-Soustrade, Isabelle; Laurent, Philippe; Gagnevin, Lionel; Jouen, Emmanuel; Pruvost, Olivier
Source:
Applied and environmental microbiology 2006 v.72 no.2 pp. 1072-1078
ISSN:
0099-2240
Subject:
plant pathogenic bacteria; random amplified polymorphic DNA technique; disease detection; blight; DNA primers; bacterial diseases of plants; Anthurium andraeanum; microbial detection; Xanthomonas axonopodis pv. dieffenbachiae; nucleotide sequences; disease diagnosis
Abstract:
... Efficient control of Xanthomonas axonopodis pv. dieffenbachiae, the causal agent of anthurium bacterial blight, requires a sensitive and reliable diagnostic tool. A nested PCR test was developed from a sequence-characterized amplified region marker identified by randomly amplified polymorphic DNA PCR for the detection of X. axonopodis pv. dieffenbachiae. Serological and pathogenicity tests were pe ...
DOI:
10.1128/AEM.72.2.1072-1078.2006
PubMed:
16461651
PubMed Central:
PMC1392979

4. Towards Efficient Isolation of R Gene Orthologs from Multiple Genotypes: Optimization of Long Range-PCR

Author:
Sanchez, M.J.; Bradeen, J.M.
Source:
Molecular breeding 2006 v.17 no.2 pp. 137-148
ISSN:
1380-3743
Subject:
Solanum bulbocastanum; genes; loci; disease resistance; blight; Phytophthora infestans; fungal diseases of plants; polymerase chain reaction; DNA; extraction; DNA primers
Abstract:
... Resistance (R) genes and the proteins they encode are key components of the defense system of plants. The exploration of R gene diversity enables the study of R gene evolution and may facilitate the isolation of new and functional alleles. Most cloned R genes occur in clusters of related sequences. Thus, the development of a tool for reliable recovery of orthologous R gene sequences to the exclusi ...
DOI:
10.1007/s11032-005-4475-5

5. PCR primers for identification of Sirococcus conigenus and S. tsugae, and detection of S. conigenus from symptomatic and asymptomatic red pine shoots

Author:
Smith, D.R.; Stanosz, G.R.
Source:
Forest pathology 2008 v.38 no.3 pp. 156-168
ISSN:
1437-4781
Subject:
DNA primers; Pinus; Sirococcus conigenus; bark; blight; conifers; detection limit; fungi; hosts; internal transcribed spacers; pathogens; polymerase chain reaction; shoots; wood; Michigan; Wisconsin
Abstract:
... Regions of diversity in the internal transcribed spacer (ITS) sequences of Sirococcus species were exploited to design primer pairs used in a PCR-based method for the identification of the conifer shoot blight pathogen Sirococcus conigenus and the closely related fungus Sirococcus tsugae. The specificity of each primer pair for the respective fungus, detection limits and utility for detection from ...
DOI:
10.1111/j.1439-0329.2007.00532.x

6. Discovery and characterization of a molecular marker for Sclerotinia minor (Jagger) resistance in peanut

Author:
Chenault, Kelly D.; Maas, Andrea L.; Damicone, John P.; Payton, Mark E.; Melouk, Hassan A.
Source:
Euphytica 2009 v.166 no.3 pp. 357-365
ISSN:
0014-2336
Subject:
Arachis hypogaea; peanuts; disease resistance; Sclerotinia minor; blight; fungal diseases of plants; genetic markers; microsatellite repeats; polymerase chain reaction; DNA primers; genotype
Abstract:
... The production of cultivated peanut, an important agronomic crop throughout the United States and the world, is consistently threatened by various diseases and pests. Sclerotinia minor Jagger (S. minor), the causal agent of Sclerotinia blight, is a major threat to peanut production in the Southwestern US, Virginia and North Carolina. Although information on the variability of morphological traits ...
Handle:
10113/27152
DOI:
10.1007/s10681-008-9816-0

7. Rapid Detection of Phytophthora nicotianae in Infected Tobacco Tissues and Soil Samples Based on Its Ypt1 Gene

Author:
Meng, Jun; Wang, Yuanchao
Source:
Phytopathologische Zeitschrift 2010 v.158 no.1 pp. 1-7
ISSN:
0931-1785
Subject:
DNA; DNA primers; Phytophthora nicotianae; blight; detection limit; disease control; disease diagnosis; fungi; genes; monitoring; plant pathogens; polymerase chain reaction; rapid methods; soil; soil sampling; tissues; tobacco
Abstract:
... This paper describes the development of a polymerase chain reaction (PCR) assay for the detection of Phytophthora nicotianae, the causal agent of Phytophthora blight of tobacco and other plants. The PCR primers were designed based on a Ras-related protein (Ypt1) gene, and 115 isolates representing 26 species of Phytophthora and 29 fungal species of plant pathogens were used to test the specificity ...
DOI:
10.1111/j.1439-0434.2009.01548.x

8. Chickpea Ascochyta Blight: Disease Status and Pathogen Mating Type Distribution in Syria

Author:
Atik, Omar; Baum, Michael; El-Ahmed, Ahmed; Ahmed, Seid; Abang, Mathew M.; Yabrak, Mohammad M.; Murad, Samer; Kabbabeh, Siham; Hamwieh, Aladdin
Source:
Phytopathologische Zeitschrift 2011 v.159 no.6 pp. 443-449
ISSN:
0931-1785
Subject:
Ascochyta rabiei; DNA primers; blight; chickpeas; pathogens; polymerase chain reaction; random mating; Syria
Abstract:
... Chickpea fields were surveyed in nine major chickpea-growing provinces of Syria in 2008 and 2009 to determine the prevalence and severity of Ascochyta blight, and the distribution of Didymella rabiei mating types (MATs) in the country. A total of 133 Ascochyta rabiei isolates were assayed for mating type, including isolates from older collections that date back to 1982. Multiplex MAT-specific PCR ...
DOI:
10.1111/j.1439-0434.2011.01788.x

9. First Report of Twig Blight Disease of Citrus Caused by Haematonectria haematococca in the Philippines

Author:
Yago J. I.; Chung K.-R.
Source:
Plant disease 2011 v.95 no.12 pp. 1590
ISSN:
0191-2917
Subject:
Citrus reticulata; DNA; DNA primers; Fusarium eumartii; Satsuma; ascospores; bark; blight; branches; chlorosis; conidia; control methods; crops; databases; defoliation; drying; fungi; girdling; industry; internal transcribed spacers; leaves; mycelium; perithecia; plant pathology; plastic bags; polymerase chain reaction; profitability; relative humidity; sequence homology; sprayers; temperature; trees; Philippines
Abstract:
... Citrus has recently become one of the most important crops in the Philippines with approximately 151,000 t of production in 2005. A new disease affected citrus (Citrus reticulata Blanco cv. Satsuma) twigs and trunks in 2005. The problem has caused significant concerns to the industry because of its destruction and high severity of infection. Symptoms include twig drying, bark hardening, twig girdl ...
DOI:
10.1094/PDIS-07-11-0569

10. Genetic diversity of the common bacterial blight pathogen of bean, Xanthomonas axonopodis pv. phaseoli, in Iran revealed by rep-PCR and PCR–RFLP analyses

Author:
Zahra Zamani; Masoud Bahar; Marie Agnès Jacques; Mohammad Reza Lak; Alireza Akhavan
Source:
World journal of microbiology & biotechnology 2011 v.27 no.10 pp. 2371-2378
ISSN:
0959-3993
Subject:
DNA; DNA primers; Xanthomonas axonopodis; bacteria; beans; blight; genetic variation; pathogens; polymerase chain reaction; restriction endonucleases; restriction fragment length polymorphism; Iran
Abstract:
... Xanthomonad-like bacteria that are associated with common bacterial blight of bean in Iran were identified on the basis of their colonial morphology, biochemical and serological properties, presence of a specific DNA fragment using PCR primers and pathogenicity on bean. Xanthomonas axonopodis pv. phaseoli (Xap) strains were further characterized using rep-PCR and restriction fragment length polymo ...
DOI:
10.1007/s11274-011-0705-7

11. Pathotype IV, a New and Highly Virulent Pathotype of Didymella rabiei, Causing Ascochyta Blight in Chickpea in Syria

Author:
Imtiaz M.; Abang M. M.; Malhotra R. S.; Ahmed S.; Bayaa B.; Udupa S. M.; Baum M.
Source:
Plant disease 2011 v.95 no.9 pp. 1192
ISSN:
0191-2917
Subject:
Ascochyta rabiei; Cicer arietinum; DNA; DNA primers; blight; breeding; chickpeas; cultivars; death; gene pool; genetic variation; loci; major genes; mating frequency; mating types; microsatellite repeats; pathogens; pathotypes; polymerase chain reaction; potting mix; virulence; wild relatives; Syria
Abstract:
... The causal agent of Ascochyta blight disease of chickpea (Cicer arietinum L.) is highly variable because of the presence of a sexual phase (Didymella rabiei). There is also selection pressure on the pathogen due to wide adoption of improved resistant chickpea cultivars in some countries. The pathogen is able to produce pathotypes with specific virulence on particular cultivars. Three pathotypes, I ...
DOI:
10.1094/PDIS-03-11-0231

12. Strain-specific SCAR markers for the detection of Trichoderma harzianum AS12-2, a biological control agent against Rhizocto nia solani, the causal agent of rice sheath blight

Author:
S. Naeimi; S. Kocsubé; Zsuzsanna Antal; S. M. Okhovvat; M. Javan-Nikkhah; C. Vágvölgyi; L. Kredics
Source:
Acta biologica Hungarica 2011 v.62 no.1 pp. 73-84
ISSN:
0236-5383
Subject:
DNA; DNA primers; Thanatephorus cucumeris; Trichoderma harzianum; biological control; biological control agents; blight; databases; fungi; paddies; population dynamics; rice; sequence homology; Iran
Abstract:
... In order to identify a specific marker for T. harzianum AS12-2, a strain capable of controlling rice sheath blight caused by Rhizoctonia solani, UP-PCR was performed using five universal primers (UP) both separately and in pairwise combinations. The application of two UP primers resulted in the amplification of unique fragments from the genomic DNA of T. harzianum AS12-2, clea ...
DOI:
10.1556/ABiol.61.2011.1.8

13. Transgenic Rice Plants Harboring Genomic DNA from Zizania latifolia Confer Bacterial Blight Resistance

Author:
Wei-wei SHEN; Cheng-li SONG; Jie CHEN; Ya-ping FU; Jian-li WU; Shao-mei JIANG
Source:
Rice science 2011 v.18 no.1 pp. 17-22
ISSN:
1672-6308
Subject:
DNA; DNA primers; Zizania latifolia; blight; chromosomes; clones; disease resistance; genes; libraries; nucleotide sequences; polymerase chain reaction; sequence analysis; transgenic plants
Abstract:
... Based on the sequence of a resistance gene analog FZ14 derived from Zizania latifolia (Griseb.), a pair of specific PCR primers FZ14P₁/FZ14P₂ was designed to isolate candidate disease resistance gene. The pooled-PCR approach was adopted using the primer pair to screen a genomic transformation-competent artificial chromosome (TAC) library derived from Z. latifolia. A positive TAC clone (ZR1) wa ...
DOI:
10.1016/S1672-6308(11)60003-6

14. First Report of Anthracnose of Tricyrtis macropoda Caused by Colletotrichum gloeosporioides in Korea

Author:
Park J. H.; Han K. S.; Kwon Y. D.; Shin H. D.
Source:
Plant disease 2012 v.96 no.7 pp. 1070
ISSN:
0191-2917
Subject:
DNA primers; Glomerella cingulata; Tricyrtis; agar; anthracnose; blight; cities; conidia; endangered species; epiphytes; etiology; flowers; forests; fungi; genetic databases; greenhouses; habitats; harvesting; herbaria; indigenous species; inoculum; internal transcribed spacers; leaf spot; leaves; managers; pathogenicity; plastic bags; polymerase chain reaction; relative humidity; ribosomal DNA; scientists; sequence homology; type collections; China; Japan; Korean Peninsula; Taiwan
Abstract:
... Tricyrtis macropoda Miq. (syn. T. dilatata Nakai), known as speckled toadlily, is a perennial herb native to China, Japan, and Korea. The plant has been highly praised for its beautiful flowers and rare populations in natural habitats. In September 2006, several dozen plants were heavily damaged by leaf spots and blight in cultivated plantings in the city of Pocheon, Korea. The infections with the ...
DOI:
10.1094/PDIS-03-12-0247-PDN

15. First Report of Southern Blight of Iresine herbstii Caused by Sclerotium rolfsii in Taiwan

Author:
Fu C. H.; Huang Y. P.; Lin F. Y.
Source:
Plant disease 2012 v.96 no.11 pp. 1692
ISSN:
0191-2917
Subject:
Athelia rolfsii; DNA primers; Iresine herbstii; agar; blight; container-grown plants; farms; fungi; growth chambers; herbaceous plants; hyphae; internal transcribed spacers; leaves; mycelium; polymerase chain reaction; ribosomal DNA; sclerotia; soil; stem rot; stems; summer; Taiwan
Abstract:
... Widely cultivated commercially, Iresine herbstii Hook is a potted herbaceous plant popular for its foliage, which varies from a dark red to brownish maroon. In the summer of 2010, a sudden wilt of I. herbstii plants was observed at a recreational farm in Taipei City in northern Taiwan. The initial symptoms were water-soaked lesions that became soft and then rotted. Necrotic areas on the stems were ...
DOI:
10.1094/PDIS-03-12-0271-PDN

16. First Report of Web Blight on Winter Savory (Satureja Montana “Repandens”) Caused by Rhizoctonia solani AG-1-IA in Italy

Author:
Garibaldi A.; Bertetti D.; Pensa P.; Amatulli M. T.; Gullino M. L.
Source:
Plant disease 2012 v.96 no.4 pp. 585
ISSN:
0191-2917
Subject:
DNA primers; Satureja montana; Thanatephorus cucumeris; agar; bark; blight; cell death; clay; culture flasks; farms; fungi; genetic databases; glass; growth chambers; hyphae; inoculum; internal transcribed spacers; leaves; mycelium; pathogenicity; pathogens; peat; plastic bags; polymerase chain reaction; pumice; ribosomal DNA; sclerotia; seeds; sequence homology; shoots; shrubs; sodium hypochlorite; steam; stems; streptomycin; sulfates; summer; wheat; Italy; Poland
Abstract:
... Satureja montana L. (winter savory “Repandens”) is an evergreen shrub. In late summer 2010, blight was observed on a farm near Albenga (northern Italy) on 3% of 500 potted 2-month-old plants. Semicircular, water-soaked lesions appeared first on stems then on leaves. As the disease progressed, blighted leaves turned brown, withered, clung to the shoots, and matted on the surrounding foliage within ...
DOI:
10.1094/PDIS-11-11-0966

17. First Confirmed Report of Cercospora Blight of Asparagus Caused by Cercospora asparagi in Korea

Author:
Han K. S.; Kim B. S.; Park J. H.; Shin H. D.
Source:
Plant disease 2013 v.97 no.3 pp. 428
ISSN:
0191-2917
Subject:
Asparagus officinalis; Cercospora; DNA primers; agar; blight; branches; conidia; conidiophores; defoliation; farms; ferns and fern allies; fungi; genetic databases; greenhouses; herbaria; humidity; internal transcribed spacers; juices; pathogenicity; phylogeny; plastic bags; polyethylene; polymerase chain reaction; ribosomal DNA; sporulation; summer; tissues; type collections; vigor; Korean Peninsula
Abstract:
... In September 2011, hundreds of asparagus (Asparagus officinalis L.) showing symptoms of blight with nearly 100% incidence (percentage of plants affected) were found in polyethylene tunnels at an organic farm in Gangneung City of Korea. Lesions on needles and branches of the ferns were small, elliptic to subcircular, pale tan to brown with reddish brown borders, and became gray and cottony due to h ...
DOI:
10.1094/PDIS-08-12-0712-PDN

18. First Report of Boscalid and Penthiopyrad-Resistant Isolates of Alternaria solani Causing Early Blight of Potato in Michigan

Author:
Miles T. D.; Fairchild K. L.; Merlington A.; Kirk W. W.; Rosenzweig N.; Wharton P. S.
Source:
Plant disease 2013 v.97 no.12 pp. 1655
ISSN:
0191-2917
Subject:
Alternaria solani; DNA primers; Solanum tuberosum; agar; blight; boscalid; conidia; cross resistance; leaves; microbial growth; mutation; mycelium; phenotype; potatoes; risk; succinate dehydrogenase (quinone); Idaho; Michigan
Abstract:
... Early blight of potato (Solanum tuberosum) is caused by Alternaria solani and occurs annually in Michigan. If left uncontrolled, it can result in yield losses exceeding 20% and impact stored potatoes. The disease is commonly managed using succinate dehydrogenase inhibitor (SDHI) fungicides (1). Unfortunately, recent studies have shown that SDHI resistance has increased dramatically over the past 2 ...
DOI:
10.1094/PDIS-01-13-0117-PDN

19. First Report of Box Blight Caused by Cylindrocladium pseudonaviculatum (C. buxicola) in British Columbia, Canada

Author:
Elmhirst J. F.; Auxier B. E.; Wegener L. A.
Source:
Plant disease 2013 v.97 no.4 pp. 559
ISSN:
0191-2917
Subject:
Buxus sempervirens; Calonectria; Cylindrocladium; DNA primers; Dianthus caryophyllus; ambient temperature; bleaching agents; blight; chlamydospores; conidia; conidiophores; defoliation; dieback; fungi; internal transcribed spacers; landscapes; leaf spot; microscopy; pathogens; petioles; planting; polymerase chain reaction; ribosomal DNA; sclerotia; sporodochia; stems; British Columbia; Europe
Abstract:
... Boxwoods (Buxus spp.) are common woody ornamental hedging plants in Europe and North America, typically propagated by cuttings. In October 2011, shoot dieback and defoliation was observed on Buxus sempervirens ‘Suffruticosa’ (dwarf English boxwood) and ‘Green Balloon’ in outdoor, 10-cm pots at a wholesale nursery in Chilliwack, British Columbia. Circular leaf spots with black rings occurred on lea ...
DOI:
10.1094/PDIS-08-12-0801-PDN

20. First Report of Camphor Tree (Cinnamomum camphora) as a Host of Phytophthora ramorum

Author:
Rooney-Latham S.; Honeycutt E.; Ochoa J.; Grünwald N. J.; Blomquist C. L.
Source:
Plant disease 2013 v.97 no.10 pp. 1377
ISSN:
0191-2917
Subject:
Cinnamomum camphora; DNA primers; Phytophthora ramorum; agar; blight; branches; camphor; chlamydospores; decline; dieback; enzyme-linked immunosorbent assay; forests; freezers; genetic databases; growth chambers; hosts; hyphae; internal transcribed spacers; juices; landscapes; leaf abscission; leaves; microsatellite repeats; pathogenicity; plant pathology; plastic bags; polymerase chain reaction; residential areas; ribosomal DNA; sporangia; summer; temperature; trees; weather; California
Abstract:
... Cinnamomum camphora (Lauraceae) is an evergreen shade tree grown in many parts of the United States, including California. From 2007 to 2011, an arborist working in a residential neighborhood in Mill Valley (Marin Co.) noticed several camphor trees with branch dieback and decline. Affected trees had patchy, irregular cankers on the branches and shoot blight. Cankers were black and most had horizon ...
DOI:
10.1094/PDIS-01-13-0073-PDN