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- Nakayasu, Chihaya, et al. Show all 11 Authors
- Vaccine 2018 v.36 no.19 pp. 2643-2649
- Infectious spleen and kidney necrosis virus; Seriola dumerili; Seriola quinqueradiata; antibodies; antigens; blood serum; case studies; enzyme-linked immunosorbent assay; fish; genes; immune response; models; pathogens; prediction; protective effect; recombinant vaccines; screening; sorbents; vaccine development
- ... Predicting antigens that would be protective is crucial for the development of recombinant vaccine using genome based vaccine development, also known as reverse vaccinology. High-throughput antigen screening is effective for identifying vaccine target genes, particularly for pathogens for which minimal antigenicity data exist. Using red sea bream iridovirus (RSIV) as a research model, we developed ...
- Nakayasu, Chihaya, et al. Show all 7 Authors
- Fish & shellfish immunology 2014 v.39 no.2 pp. 396-400
- 3-hydroxyacyl-CoA dehydrogenase; Escherichia coli; Flavobacterium psychrophilum; H+/K+-exchanging ATPase; H-transporting ATP synthase; Plecoglossus altivelis; antibodies; antigens; bacterial cold-water disease; blood serum; fish; fluorescent antibody technique; glutamate dehydrogenase; immune response; recombinant proteins; surface proteins; vaccine development
- ... Protective efficacies of three antigenic proteins (3-hydroxyacyl-CoA dehydrogenase (HCD), ATP synthase beta subunit (atpD), and glutamate dehydrogenase (gdhA)) against Flavobacterium psychrophilum were investigated in ayu (Plecoglossus altivelis). Recombinant proteins of HCD, atpD, and gdhA were expressed in Escherichia coli BL21 cells. Ayu were then vaccinated with inactivated cells via the intra ...