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- Poliak, Sylvie, et al. Show all 7 Authors
- Veterinary microbiology 2009 v.137 no.1-2 pp. 156-164
- zoonoses; Brucella; brucellosis; disease detection; disease diagnosis; polymerase chain reaction; new methods; rapid methods; DNA probes; genes; transposons; ligases; molecular epidemiology; pathogen identification; validity; accuracy; phylogeny; molecular genetics
- ... Culture is considered as the reference standard assay for diagnosis of Brucella spp. in humans and animals but it is time-consuming and hazardous. In this study, we evaluated the performances of newly designed real-time PCR assays using TaqMan® probes and targeting the 3 following specific genes: (i) the insertion sequence IS711, (ii) bcsp31 and (iii) per genes for the detection of Brucella at gen ...