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1. Difference of EGCg adhesion on cell surface between Staphylococcus aureus and Escherichia coli visualized by electron microscopy after novel indirect staining with cerium chloride

Author:
Nakayama, Motokazu,   et al.   ; Shigemune, Naofumi; Tsugukuni, Takashi; Tokuda, Hajime; Miyamoto, Takahisa;     Show all 5 Authors
Source:
Journal of microbiological methods 2011 v.86 no.1 pp. 97-103
ISSN:
0167-7012
Subject:
Escherichia coli; Staphylococcus aureus; bacteria; catechin; cell adhesion; cerium; epigallocatechin; hydrogen peroxide; pH; polystyrenes; transmission electron microscopy
Abstract:
... We developed a novel method using indirect staining with cerium chloride for visualization of the catechin derivative epigallocatechin gallate (EGCg) on the surface of particles, i.e., polystyrene beads and bacterial cells, by electron microscopy. The staining method is based on the fact that in an alkaline environment, EGCg produces hydrogen peroxide, and then hydrogen peroxide reacts with cerium ...
DOI:
10.1016/j.mimet.2011.04.010