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- Nakayama, Motokazu, et al. Show all 5 Authors
- Journal of microbiological methods 2011 v.86 no.1 pp. 97-103
- Escherichia coli; Staphylococcus aureus; bacteria; catechin; cell adhesion; cerium; epigallocatechin; hydrogen peroxide; pH; polystyrenes; transmission electron microscopy
- ... We developed a novel method using indirect staining with cerium chloride for visualization of the catechin derivative epigallocatechin gallate (EGCg) on the surface of particles, i.e., polystyrene beads and bacterial cells, by electron microscopy. The staining method is based on the fact that in an alkaline environment, EGCg produces hydrogen peroxide, and then hydrogen peroxide reacts with cerium ...
- Nakayama, Motokazu, et al. Show all 8 Authors
- Food control 2012 v.25 no.1 pp. 225-232
- Escherichia coli O157; Staphylococcus aureus; bacteria; catechin; electron microscopes; enzyme activity; enzyme substrates; green tea; polyacrylamide gel electrophoresis; secretion; sodium chloride; surface proteins; survival rate
- ... The mechanism of the combined anti-bacterial effect of green tea extract (GTE) and NaCl against Staphylococcus aureus NBRC 13276 and Escherichia coli O157:H7 was investigated. After treatment for 1 h, GTE was more effective against S. aureus than E. coli O157:H7, and combined GTE/NaCl treatment caused greater cellular damage in S. aureus NBRC 13276, where it was bactericidal, than E. coli O157:H7. ...