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- Liotta Janice L.; Lee Alice C. Y.; Koompapong Khuanchai N.; Yaros Joseph P.; Prullage Joseph; Ulrich Michael A.; Bowman Dwight D.
- Journal of parasitology 2012 v.98 no.5 pp. 1037-1038
- Ancylostoma tubaeforme; DNA; cats; death; eggs; feces; harvesting; larvae; necropsy; puppies; restriction fragment length polymorphism; sequence analysis; specific pathogen-free animals; sugars; Florida
- ... Because the eggs of Ancylostoma species of dogs and cats are difficult to readily distinguish morphologically, isolation of a certain species often requires the humane death of the source animal or holding an animal after treatment to obtain worms for specific identification or to harvest ex utero eggs. The objective of this study was to obtain an isolate of Ancylostoma braziliense from 1-time, fi ...
- Liotta Janice L.; Koompapong Khuanchai N.; Yaros Joseph P.; Prullage Joseph; Bowman Dwight D.
- Journal of parasitology 2012 v.98 no.5 pp. 1032-1033
- Ancylostoma tubaeforme; DNA; cats; eggs; feces; hookworms; polymerase chain reaction; restriction fragment length polymorphism; sugars; Florida
- ... A convenience sampling of fecal specimens from 40 cats in northern Florida was examined for the presence of Ancylostoma braziliense eggs by using centrifugal sugar flotation and polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). Of the 40 samples, 26 (65%) contained hookworm eggs. DNA from 24 samples was successfully amplified using PCR; using RFLP, 10 samples were id ...