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1. 3,4,5-O-tricaffeoylquinic acid with anti-radiation activity suppresses LPS-induced NLRP3 inflammasome activation via autophagy in THP-1 macrophages

Author:
Jiajun Liu; Jingyun Chen; Baixue Xu; Long Lin; Shaoqun Liu; Xiaoying Ma; Jianwen Liu
Source:
Molecular immunology 2022 v.147 pp. 187-198
ISSN:
0161-5890
Subject:
Azolla; adverse effects; autophagosomes; autophagy; caspase-1; cell lines; cesium radioisotopes; chloroquine; flow cytometry; humans; immune response; inflammasomes; inflammation; lipopolysaccharides; macrophages; proteasome inhibitors; radiotherapy; secretion
Abstract:
... Damage to normal tissues caused by excessive ionizing radiation (IR) exposure is the major side effect of radiotherapy. Several recent studies have shown that IR-induced damage to tissues leads to a systemic immune response and NLRP3 inflammasome activation in immune cells. 3,4,5-O-tricaffeoylquinic acid (tCQA), extracted from the natural plant Azolla imbricata, relieves inflammation and has radio ...
DOI:
10.1016/j.molimm.2022.05.011

2. A blueprint from nature: miRNome comparison of plasma cells and CHO cells to optimize therapeutic antibody production

Author:
Nadja Raab; Nikolas Zeh; Patrick Schlossbauer; Sven Mathias; Benjamin Lindner; Anna Stadermann; Martin Gamer; Simon Fischer; Karlheinz Holzmann; René Handrick; Kerstin Otte
Source:
New biotechnology 2022 v.66 pp. 79-88
ISSN:
1871-6784
Subject:
Cricetulus griseus; antibody formation; biopharmaceuticals; bioprocessing; biotechnology; cell lines; fatty acid metabolism; gene expression; humans; industry; lysine; mice; microRNA; monoclonal antibodies; prediction; product quality; secretion; therapeutics; transcriptomics
Abstract:
... Chinese Hamster Ovary (CHO) cells are the most frequently used biopharmaceutical production hosts, although industry is presently suffering from their variable recombinant product quality, insufficient long-term stability and low productivity. Here, we present an effort to address overall cell line engineering by a novel bottom-up microRNA (miRNA) screening approach. miRNAs are small non-coding RN ...
DOI:
10.1016/j.nbt.2021.10.005

3. A rapid homogenous bioassay for detection of thyroid-stimulating antibodies based on a luminescent cyclic AMP biosensor

Author:
Lynn Yihong Miao; Hannah J. Kim; Kindra Whitlatch; Depesh Jaiswal; Adriana Navarro; Richard Egan; Paul D. Olivo
Source:
Journal of immunological methods 2022 v.501 pp. 113199
ISSN:
0022-1759
Subject:
autoimmune diseases; bioassays; biosensors; blood; cell culture; cell lines; cyclic AMP; humans; luciferase; luminescence; monoclonal antibodies
Abstract:
... Graves' disease (GD) is an autoimmune disease caused by antibodies to the thyroid stimulating hormone receptor (TSHR). The FDA-cleared Thyretain™ TSI bioassay is a highly specific method to detect thyroid stimulating antibodies (TSAb/TSI) in the blood of patients with autoimmune thyroid disease (AITD), particularly GD. To simplify the workflow of this bioassay and to support a semi-quantitative re ...
DOI:
10.1016/j.jim.2021.113199

4. A rapid spectrophotometric method to identify inhibitors of human erythropoiesis

Author:
Denise P. Ilboudo; Sarah D'Alessandro; Silvia Parapini; Estefanía Calvo Alvarez; Paola Misiano; Donatella Taramelli; Nicoletta Basilico
Source:
Journal of pharmacological and toxicological methods 2022 v.113 pp. 107134
ISSN:
1056-8719
Subject:
absorbance; artemisinin; carcinogenicity; carcinogens; cell lines; erythropoiesis; hemoglobin; humans; microscopy; models; mutagens; solubilization; spectrophotometers; toxicology
Abstract:
... Erythropoiesis is a complex physiological process by which erythroid progenitors proliferate and differentiate into nonnucleated red blood cells. Several methods can be used to monitor in vitro the differentiation of erythroid precursors, and hence the toxic effects of drugs, chemicals, or pollutants. One of the most commonly available assay of erythropoiesis is the microscopic observation of diff ...
DOI:
10.1016/j.vascn.2021.107134

5. Accurate identification of circRNA landscape and complexity reveals their pivotal roles in human oligodendroglia differentiation

Author:
Yangping Li; Feng Wang; Peng Teng; Li Ku; Li Chen; Yue Feng; Bing Yao
Source:
Genome biology 2022 v.23 no.1 pp. 48
ISSN:
1474-760X
Subject:
brain; cell lines; exons; gene expression; humans; introns; landscapes; microRNA; neurons; oligodendroglia; ribonucleases
Abstract:
... BACKGROUND: Circular RNAs (circRNAs), a novel class of poorly conserved non-coding RNAs that regulate gene expression, are highly enriched in the human brain. Despite increasing discoveries of circRNA function in human neurons, the circRNA landscape and function in developing human oligodendroglia, the myelinating cells that govern neuronal conductance, remains unexplored. Meanwhile, improved expe ...
DOI:
10.1186/s13059-022-02621-1

6. Adverse pulmonary impacts of environmental concentrations of oil mist particulate matter in normal human bronchial epithelial cell

Author:
Chia-Hsiang Lai; Yi-Chun Chen; Kun-Yi Andrew Lin; Yi-Xian Lin; Tsung-Han Lee; Chia-Hua Lin
Source:
Science of the total environment 2022 v.809 pp. 151119
ISSN:
0048-9697
Subject:
cell lines; cell viability; cytokines; environment; epithelial cells; epithelium; humans; lungs; mists; occupational exposure; oils; oxidative stress; particle size; particulates; respiratory tract diseases; risk factors; tight junctions; toxicity
Abstract:
... Airborne oil mist particulate matter (OMPM) is generated during industrial processes such as metalworking and may be associated with pulmonary dysfunction. In this study, we employed the normal human bronchial epithelial BEAS-2B cell line to elucidate the association between pulmonary toxicity and OMPM of 2.5–10 μm, 1.0–2.5 μm and <1.0 μm particle sizes (OMPM₁₀–₂.₅, OMPM₂.₅–₁.₀ and OMPM₁.₀). We me ...
DOI:
10.1016/j.scitotenv.2021.151119

7. Aloe vera and Fermented Extracts Exhibit an Anti-Inflammatory Effect on Human Glioblastoma/Astrocytoma U373 MG Cells

Author:
Antonio Tornero-Martínez; Maria del Carmen Silva-Lucero; Edén Cano Sampedro; Eva Ramón-Gallegos; Claudia Pérez-Cruz; Brenda Pérez-Grijalva; Rosalva Mora-Escobedo
Source:
Plant foods for human nutrition 2022 v.77 no.1 pp. 37-43
ISSN:
0921-9668
Subject:
Aloe vera; anti-inflammatory activity; astrocytes; cell lines; cell viability; digestion; fermentation; glioblastoma; human nutrition; humans; inflammation; interleukin-6; mitogen-activated protein kinase; polysaccharides; substance P
Abstract:
... The anti-inflammatory effects of Aloe vera (AV), polysaccharide extract from AV, and extracts from the digestion and colonic fermentation of AV were evaluated using an immortal astrocyte cell line (U373 MG) that develops a neuro-inflammatory profile. Cell viability and inflammatory markers were assessed after stimulation with neuropeptide substance P (SP) that activates the pro-inflammatory MAPK ( ...
DOI:
10.1007/s11130-022-00957-4

8. Alternatones A and B, two polyketides possessing novel skeletons from entophyte Alternaria alternate L-10

Author:
Xuan Zhang; Xian-Xian Liu; Yin-Nan Xing; Mei Zhang; Yang Zhao; Yuan-Yuan Wei; Bo Zhang; Rui-Hua Jiao
Source:
Journal of Asian natural products research 2022 v.24 no.4 pp. 353-360
ISSN:
1477-2213
Subject:
Alternaria alternata; X-ray diffraction; biosynthesis; carcinoma; cell lines; cytotoxicity; hepatoma; humans; polyketides; research; spectroscopy
Abstract:
... On our ongoing searching for bioactive natural products derived from entophytes, two polyketides possessing novel skeletons, alternatones A–B (1–2), were identified from the culture of Alternaria alternate L-10. Their structures were established by a combination of spectroscopic and single-crystal X-ray diffraction with Cu Ka radiation. Alternatone A (1) exhibited cytotoxic activity against human ...
DOI:
10.1080/10286020.2021.1935893

9. An In Vitro and In Silico Study of Antioxidant Properties of Curcuminoid N-alkylpyridinium Salts: Initial Assessment of Their Antitumoral Properties

Author:
Oscar Forero-Doria; Luis Guzmán; Felipe Jiménez-Aspee; Javier Echeverría; Sergio Wehinger; Claudio Valenzuela; Ramiro Araya-Maturana; Maximiliano Martínez-Cifuentes
Source:
Antioxidants 2022 v.11 no.6 pp. -
ISSN:
2076-3921
Subject:
antioxidant activity; breast neoplasms; cell lines; cell movement; cell viability; colorectal neoplasms; computer simulation; curcumin; density functional theory; dissociation; enthalpy; fibroblasts; free radicals; humans; ionic liquids; mice; models; moieties; pyridines; solvation
Abstract:
... In this work, we report the synthesis of curcuminoids with ionic liquid characteristics, obtained by incorporating alkyl-substituted pyridinium moiety rather than one phenyl group through a two-step process. The antioxidant capacity of the obtained compounds was evaluated in vitro by 1,1-diphenyl-picrylhydrazyl (DPPH) free radical scavenging and ferric reducing antioxidant power (FRAP) assays, sho ...
DOI:
10.3390/antiox11061104

10. Anethum Graveolens Essential Oil Nanoemulsions (AGEO-NE) as an Exclusive Apoptotic Inducer in Human Lung Adenocarcinoma (A549) Cells

Author:
Haleh Sadat Tavakkol Afshari; Masoud Homayouni Tabrizi; Touran Ardalan; Neda Jalili Anoushirvani; Roya Mahdizadeh
Source:
Nutrition and cancer 2022 v.74 no.4 pp. 1411-1419
ISSN:
1532-7914
Subject:
Anethum graveolens; adenocarcinoma; antineoplastic agents; antioxidant activity; antioxidants; apoptosis; biocompatibility; cell lines; cell viability; cytotoxicity; encapsulation; epithelium; essential oils; fibroblasts; flow cytometry; gene expression; humans; lung neoplasms; lungs; nanoemulsions; nutrition; ultrasonic treatment
Abstract:
... Anethum graveolens essential oil (AGEO) is used as an anticancer compound that can be made more efficacious by improving its biosolubility and biocompatibility. In the current study, we aimed to improve AGEO bioactivity using the nanoemulsion-based encapsulation technique. To formulate stable AGEO-NE, an ultrasonication method was utilized. The size, stability, and morphology of the AGEO-NE was me ...
DOI:
10.1080/01635581.2021.1952450

11. Antibacterial, Antioxidant and Melanogenesis Inhibitory Activity of Auraptene, a Coumarin from Ferula szowitsiana Root

Author:
Ensiyeh Charmforoshan; Ehsan Karimi; Ehsan Oskoueian; Mehrdad Iranshahi
Source:
Nutrition and cancer 2022 v.74 no.5 pp. 1829-1836
ISSN:
1532-7914
Subject:
Clostridium perfringens; Escherichia coli; Ferula; Salmonella Typhimurium; Staphylococcus aureus; antibacterial properties; antioxidant activity; catalase; cell lines; coumarin; enzyme inhibition; fibroblasts; functional foods; glutathione peroxidase; humans; melanogenesis; melanoma; mice; nitric oxide; nutrition; superoxide dismutase; thiobarbituric acid; thiocyanates; Central Asia; Iran
Abstract:
... The auraptene is a geranyloxyn coumarin found in the Ferula species. The plant is endemic in Central Asia and it is used as a medicinal food in Iran. This research aimed to evaluate the antibacterial, antioxidant, and anti-melanogenic properties of auraptene, a coumarin from Ferula szowitsiana root. The results revealed that auraptene possessed antibacterial activity with minimum inhibitory and mi ...
DOI:
10.1080/01635581.2021.1962922

12. Antibiofilm and anticancer activities of unripe and ripe Azadirachta indica (neem) seed extracts

Author:
Kartik Chandra Guchhait; Tuhin Manna; Manas Barai; Monalisha Karmakar; Sourav Kumar Nandi; Debarati Jana; Aditi Dey; Suman Panda; Priyanka Raul; Anuttam Patra; Rittwika Bhattacharya; Subhrangsu Chatterjee; Amiya Kumar Panda; Chandradipa Ghosh
Source:
BMC complementary medicine and therapies 2022 v.22 no.1 pp. 42
ISSN:
2662-7671
Subject:
Azadirachta indica; Staphylococcus aureus; Vibrio cholerae; acridine orange; alternative medicine; antibacterial properties; antibiotics; antineoplastic activity; bacterial culture; biofilm; breast neoplasms; cell lines; cytotoxicity; ethidium; fluorescence microscopy; gas chromatography; humans; mass spectrometry; methanol; minimum inhibitory concentration; multiple drug resistance; neoplasm cells; phthalic acid; toxicity testing; triazines
Abstract:
... BACKGROUND: Antibiotic resistances of pathogens and breast cancer warrant the search for new alternative strategies. Phytoextracts can eradicate microbe-borne diseases as well as cancer with lower side effects compared to conventional antibiotics. AIM: Unripe and ripe Azadirachta indica (neem) seed extracts were explored as potential antibiofilm and anticancer agents in combating multidrug-resista ...
DOI:
10.1186/s12906-022-03513-4

13. Anticancer Effects of New Ceramides Isolated from the Red Sea Red Algae Hypnea musciformis in a Model of Ehrlich Ascites Carcinoma: LC-HRMS Analysis Profile and Molecular Modeling

Author:
Sameh S. Elhady; Eman S. Habib; Reda F. A. Abdelhameed; Marwa S. Goda; Reem M. Hazem; Eman T. Mehanna; Mohamed A. Helal; Khaled M. Hosny; Reem M. Diri; Hashim A. Hassanean; Amany K. Ibrahim; Enas E. Eltamany; Usama Ramadan Abdelmohsen; Safwat A. Ahmed
Source:
Marine drugs 2022 v.20 no.1 pp. -
ISSN:
1660-3397
Subject:
Hypnea; adenocarcinoma; apoptosis; ascites; blood serum; breasts; caspase-3; cell lines; ceramides; cytotoxicity; doxorubicin; electrospray ionization mass spectrometry; hexadecenoic acid; humans; ketosteroids; liquid chromatography; mice; necrosis; phytochemicals; stearic acid; sterols; terpenoids; vascular endothelial growth factor B; Red Sea
Abstract:
... Different classes of phytochemicals were previously isolated from the Red Sea algae Hypnea musciformis as sterols, ketosteroids, fatty acids, and terpenoids. Herein, we report the isolation of three fatty acids—docosanoic acid 4, hexadecenoic acid 5, and alpha hydroxy octadecanoic acid 6—as well as three ceramides—A (1), B (2), and C (3)—with 9-methyl-sphinga-4,8-dienes and phytosphingosine bases. ...
DOI:
10.3390/md20010063

14. Antimicrobial activity, in vitro anticancer effect (MCF-7 breast cancer cell line), antiangiogenic and immunomodulatory potentials of Populus nigra L. buds extract

Author:
Brigitta Kis; Ioana Zinuca Pavel; Stefana Avram; Elena Alina Moaca; Martina Herrero San Juan; Anja Schwiebs; Heinfried H. Radeke; Delia Muntean; Zorita Diaconeasa; Daliana Minda; Camelia Oprean; Florina Bojin; Cristina Adriana Dehelean; Codruta Soica; Corina Danciu
Source:
BMC complementary medicine and therapies 2022 v.22 no.1 pp. 74
ISSN:
2662-7671
Subject:
Populus nigra; alternative medicine; antimicrobial properties; antineoplastic activity; antioxidant activity; apoptosis; breast neoplasms; cell cycle checkpoints; cell lines; cell viability; chemical constituents of plants; chorioallantoic membrane; dose response; humans; immunomodulators; interleukin-12; interleukin-23; neoplasm cells; toxicity; Romania
Abstract:
... PURPOSE: The aim of this study was to evaluate the antioxidant potential, antimicrobial activity, the in vitro anticancer effect (tested on MCF-7 breast cancer cell line), as well as the antiangiogenic and immunomodulatory potential of Populus nigra L. bud (Pg) extract collected from the western part of Romania. RESULTS: Populus nigra L. bud extract presents an important antioxidant activity, due ...
DOI:
10.1186/s12906-022-03526-z

15. Antioxidant Activity of Resveratrol Diastereomeric Forms Assayed in Fluorescent-Engineered Human Keratinocytes

Author:
Ilaria Bononi; Paola Tedeschi; Vanessa Mantovani; Annalisa Maietti; Elisa Mazzoni; Cecilia Pancaldi; Vincenzo Brandolini; Mauro Tognon
Source:
Antioxidants 2022 v.11 no.2 pp. -
ISSN:
2076-3921
Subject:
Scyphozoa; Vitis vinifera; antioxidant activity; antioxidants; cell lines; cell proliferation; cytotoxicity; fluorescence; free radicals; grapes; green fluorescent protein; human nutrition; humans; keratinocytes; models; oxidants; oxidative stress; resveratrol
Abstract:
... Resveratrol is a powerful antioxidant molecule. In the human diet, its most important source is in Vitis vinifera grape peel and leaves. Resveratrol exists in two isoforms, cis- and trans. The diastereomeric forms of many drugs have been reported as affecting their activity. The aim of this study was to set up a cellular model to investigate how far resveratrol could counteract cytotoxicity in an ...
DOI:
10.3390/antiox11020196

16. Antioxidant, antimicrobial and anticarcinogenic activities of bovine milk proteins and their hydrolysates - A review

Author:
Marika Bielecka; Grażyna Cichosz; Hanna Czeczot
Source:
International dairy journal 2022 v.127 pp. 105208
ISSN:
0958-6946
Subject:
anticarcinogenic activity; breast neoplasms; cell lines; health promotion; homeostasis; humans; hydrolysis; immunostimulants; lactoferrin; lung neoplasms; lysozyme; metastasis; milk; neoplasm cells; osteosarcoma; peptides; peroxidase; prostatic neoplasms
Abstract:
... Milk proteins and the products of their hydrolysis are characterised by high levels of biological activity. The health-promoting potential of bovine milk proteins, peptides and amino acids can be attributed to their antioxidant, anti-inflammatory and immunostimulatory properties. Milk proteins, peptides and enzymes play a role in the maintenance of prooxidant/antioxidant homeostasis in the human b ...
DOI:
10.1016/j.idairyj.2021.105208

17. Antiproliferative effects of metformin in cellular models of pheochromocytoma

Author:
Cinthia Gabriel Meireles; Caroline Lourenço de Lima; Marcela Martins de Paula Oliveira; Rafael Abe da Rocha Miranda; Lisa Romano; Teisha Yo-Stella Brashaw; Eliete Neves da Silva Guerra; Francisco de Assis Rocha Neves; J. Paul Chapple; Luiz Alberto Simeoni; Adriana Lofrano-Porto
Source:
Molecular and cellular endocrinology 2022 v.539 pp. 111484
ISSN:
0303-7207
Subject:
adrenal medulla; cell lines; cell proliferation; cell respiration; cell viability; endocrinology; humans; membrane potential; metformin; mitochondrial membrane; neoplasm cells; oxygen consumption; phosphorylation; rats
Abstract:
... Pheochromocytomas (PCCs) are rare neuroendocrine tumors derived from adrenal medulla chromaffin cells. Malignancy and recurrence are rare but demand effective treatment. Metformin exerts antiproliferative effects in several cancer cell lines. We thus evaluated the effects of metformin on cell viability and proliferation, cellular respiration and AMPK-AKT-mTOR-HIFA proliferation pathway on a rat PC ...
DOI:
10.1016/j.mce.2021.111484

18. Apigenin inhibits migration and induces apoptosis of human endometrial carcinoma Ishikawa cells via PI3K-AKT-GSK-3β pathway and endoplasmic reticulum stress

Author:
Yan-Cui Liang; Qian Zhong; Run-Hui Ma; Zhi-Jing Ni; Kiran Thakur; Mohammad Rizwan Khan; Rosa Busquets; Jian-Guo Zhang; Zhao-Jun Wei
Source:
Journal of functional foods 2022 v.94 pp. 105116
ISSN:
1756-4646
Subject:
adjuvants; apigenin; apoptosis; calcium; cancer therapy; carcinoma; caspases; cell lines; cell movement; dietary supplements; drug therapy; endometrium; endoplasmic reticulum; endoplasmic reticulum stress; humans; toxicity
Abstract:
... Several experimental and biological studies have emphasized the tumor suppression efficacy and low toxicity of Apigenin (API); however, its exact underlying mechanism on human endometrial carcinoma Ishikawa cell line (EC) is still unknown. We found that API could inhibit the proliferation of Ishikawa cells at IC₅₀ of 45.55 μM, arrest the cell cycle at G2/M phase, induce apoptosis by inhibiting Bcl ...
DOI:
10.1016/j.jff.2022.105116

19. Apoptotic, antioxidant and cytotoxic properties of synthesized AgNPs using green tea against human testicular embryonic cancer stem cells

Author:
Fahimeh Mobaraki; Mohsen Momeni; Maliheh Jahromi; Farshad Moharrami Kasmaie; Maryam Barghbani; Mohammad Ehsan Taghavizadeh Yazdi; Zahra Meshkat; Fatemeh Homaee Shandiz; Seyed Mousalreza Hosseini
Source:
Process biochemistry 2022 v.119 pp. 106-118
ISSN:
1359-5113
Subject:
Camellia sinensis; acridine orange; antioxidants; apoptosis; biosynthesis; caspase-3; cell lines; cost effectiveness; cytotoxicity; dose response; green tea; humans; inhibitory concentration 50; nanosilver; propidium; testes; ultraviolet-visible spectroscopy
Abstract:
... Silver nanoparticles (AgNPs) are known as carriers of anticancer activities for effectual target treatment of cancer cells. Plant-mediated synthesis for AgNPs synthesis is valuable for biomedical applications due to their more safety and cost-effectiveness. In this work, AgNPs were synthesized successfully using green tea (Camellia sinensis) extract (CSE-AgNPs). The physicochemical properties of C ...
DOI:
10.1016/j.procbio.2022.05.021

20. Bacterial expression and purification of human matrix metalloproteinase-3 using affinity chromatography

Author:
Bolt Alexander J.; Do Linh D.; Raeeszadeh-Sarmazdeh Maryam
Source:
Journal of visualized experiments 2022 no.181 pp. e63263
ISSN:
1940-087X
Subject:
Escherichia coli; active sites; affinity chromatography; cardiovascular diseases; cell lines; codons; cytokines; equipment; extracellular matrix; humans; plasmids; protein synthesis; recombinant proteins; therapeutics
Abstract:
... Matrix metalloproteinases (MMPs) belong to the family of metzincin proteases with central roles in extracellular matrix (ECM) degradation and remodeling, as well as interactions with several growth factors and cytokines. Overexpression of specific MMPs is responsible in several diseases such as cancer, neurodegenerative diseases, and cardiovascular disease. MMPs have been the center of attention r ...
DOI:
10.3791/63263

21. Berberine-loaded liquid crystalline nanoparticles inhibit non-small cell lung cancer proliferation and migration in vitro

Author:
Keshav R. Paudel; Meenu Mehta; Geena Hew Suet Yin; Lee Li Yen; Vamshikrishna Malyla; Vyoma K. Patel; Jithendra Panneerselvam; Thiagarajan Madheswaran; Ronan MacLoughlin; Niraj Kumar Jha; Piyush Kumar Gupta; Sachin Kumar Singh; Gaurav Gupta; Pradeep Kumar; Brian G. Oliver; Philip M. Hansbro; Dinesh Kumar Chellappan; Kamal Dua
Source:
Environmental science and pollution research 2022 v.29 no.31 pp. 46830-46847
ISSN:
0944-1344
Subject:
berberine; bioavailability; cell lines; epithelium; humans; liquids; lung neoplasms; lungs; neoplasm cells; neoplasm progression; particle size; pollution; research; tetrazolium; therapeutics; water solubility
Abstract:
... Non-small cell lung cancer (NSCLC) is reported to have a high incidence rate and is one of the most prevalent types of cancer contributing towards 85% of all incidences of lung cancer. Berberine is an isoquinoline alkaloid which offers a broad range of therapeutical and pharmacological actions against cancer. However, extremely low water solubility and poor oral bioavailability have largely restri ...
DOI:
10.1007/s11356-022-19158-2

22. Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents

Author:
Marija Ivanov; Jelena Božunović; Uroš Gašić; Danijela Drakulić; Milena Stevanović; Nemanja Rajčević; Dejan Stojković
Source:
Industrial crops and products 2022 v.175 pp. 114260
ISSN:
0926-6690
Subject:
Salvia nemorosa; Staphylococcus lugdunensis; antioxidant activity; antioxidants; biofilm; butanol; cell lines; cell membranes; chemical composition; cytotoxicity; epigallocatechin gallate; ethanol; humans; immunomodulation; keratinocytes; mechanism of action; mitochondria; polyphenols; rosmarinic acid; solvents; therapeutics
Abstract:
... Salvia nemorosa L. is a rich source of bioactive constituents. We have used different solvents to obtain 14 different extracts in order to evaluate solvents impact on S. nemorosa inflorescences bioactivities. Firstly, the extraction yield, chemical composition and cytotoxic properties towards HaCaT cell line were elaborated. Afterwards, we have studied the extracts bioactivity in terms of wound he ...
DOI:
10.1016/j.indcrop.2021.114260

23. Biochemical and biophysical characterization of PADI4 supports its involvement in cancer

Author:
José L. Neira; Salomé Araujo-Abad; Ana Cámara-Artigas; Bruno Rizzuti; Olga Abian; Ana Marcela Giudici; Adrian Velazquez-Campoy; Camino de Juan Romero
Source:
Archives of biochemistry and biophysics 2022 v.717 pp. 109125
ISSN:
0003-9861
Subject:
arginine; biophysics; calcium; carcinogenesis; cell differentiation; cell lines; citrulline; computer simulation; granulocytes; histones; humans; macrophages; molecular dynamics; monocytes; neoplasm cells; pH; protein-arginine deiminase
Abstract:
... PADI4 (protein-arginine deiminase, also known as protein l-arginine iminohydrolase) is one of the human isoforms of a family of Ca²⁺-dependent proteins catalyzing the conversion of arginine to citrulline. Although the consequences of this process, known as citrullination, are not fully understood, all PADIs have been suggested to play essential roles in development and cell differentiation. They h ...
DOI:
10.1016/j.abb.2022.109125

24. Bioinformatics and Experimental Analyses Reveal NFIC as an Upstream Transcriptional Regulator for Ischemic Cardiomyopathy

Author:
Yang Ye; Qiao Jin; Qian Gong; Aoqi Li; Minghao Sun; Sibo Jiang; Yulan Jin; Zhe Zhang; Jin He; Lenan Zhuang
Source:
Genes 2022 v.13 no.6 pp. -
ISSN:
2073-4425
Subject:
bioinformatics; cardiomyocytes; cardiomyopathy; cell lines; chromatin; coronary artery disease; data collection; gene expression regulation; genes; heart failure; humans; microarray technology; muscle contraction; myocardial infarction; rats; sequence analysis; smooth muscle; therapeutics; transcription (genetics); transcription factors
Abstract:
... Ischemic cardiomyopathy (ICM) caused by coronary artery disease always leads to myocardial infarction and heart failure. Identification of novel transcriptional regulators in ICM is an effective method to establish new diagnostic and therapeutic strategies. In this study, we used two RNA-seq datasets and one microarray dataset from different studies, including 25 ICM and 21 non-failing control (NF ...
DOI:
10.3390/genes13061051

25. Biosafety-inspired structural optimization of triazolium ionic liquids based on structure-toxicity relationships

Author:
Xiujiao Pan; Lingzhi Li; Hsin-Heng Huang; Jialong Wu; Xiaoxia Zhou; Xiliang Yan; Jianbo Jia; Tongtao Yue; Yen-Ho Chu; Bing Yan
Source:
Journal of hazardous materials 2022 v.424 pp. 127521
ISSN:
0304-3894
Subject:
apoptosis; aquatic environment; cations; cell cycle checkpoints; cell lines; cell membranes; cytotoxicity; human health; humans; hydrophobicity; imidazoles; intestines; kidneys; lipidomics; liver; nitrogen; oral exposure; stomach; vapor pressure
Abstract:
... Ionic liquids (ILs), owing to their low vapor pressure and excellent solvating ability, are being increasingly applied in various industries to replace highly toxic organic solvents. They mainly pollute aquatic environment and soils, directly endangering eco-environment and human health. Therefore, it is critical to understand and optimize structural motifs of ILs with reduced toxicity. Considerin ...
DOI:
10.1016/j.jhazmat.2021.127521

26. CCL5 secreted by luminal B breast cancer cells induces polarization of M2 macrophages through activation of MEK/STAT3 signaling pathway via CCR5

Author:
Yong-yun Zhu; Ying-chun Zhao; Chuang Chen; Min Xie
Source:
Gene 2022 v.812 pp. 146100
ISSN:
0378-1119
Subject:
breast neoplasms; cancer therapy; cell lines; genes; humans; ligands; macrophages; metastasis; monocytes; mortality; neoplasm cells; phosphorylation
Abstract:
... In humans, breast cancer affects a large number of females and causes a high rate of mortality worldwide. Chemokine (C-C motif) ligand 5 (CCL5) is one of the cytokines that is highly correlated to the invasive and metastatic stages of breast cancer. Our previous study has suggested the prognostic value of CCL5 expression in luminal B (HER2 −) breast cancer. In this study, CCL5 expression was upreg ...
DOI:
10.1016/j.gene.2021.146100

27. Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity

Author:
Sanja Kostić; Aleksandra Vilotić; Andrea Pirković; Dragana Dekanski; Sunčica Borozan; Mirjana Nacka-Aleksić; Svetlana Vrzić-Petronijević; Milica Jovanović Krivokuća
Source:
Food and chemical toxicology 2022 v.163 pp. 112993
ISSN:
0278-6915
Subject:
DNA damage; antioxidant activity; caffeic acid; cell lines; cytotoxicity; diet; direct contact; genotoxicity; humans; lipid peroxidation; mutagens; oxidative stress; pregnancy; toxicology; trophoblast; uterine tissue
Abstract:
... Caffeic acid is highlighted as one of the major phenolic compounds present in foods with known antioxidant activity. This phenolic is among commonly consumed substances in everyday diet of pregnant women. However, there is not enough information on its effects during pregnancy, especially the most vulnerable early stage. Extravillous trophoblast cells are specific cells of the placenta that come i ...
DOI:
10.1016/j.fct.2022.112993

28. Casticin and chrysosplenol D from Artemisia annua L. induce apoptosis by inhibiting topoisomerase IIα in human non-small-cell lung cancer cells

Author:
Chunqing Fu; Keyu Zhang; Manyuan Wang; Feng Qiu
Source:
Phytomedicine 2022 v.100 pp. 154095
ISSN:
0944-7113
Subject:
Artemisia annua; DNA; DNA damage; RNA; apoptosis; cell lines; cell viability; cytotoxicity; dose response; flow cytometry; fluorescent antibody technique; gene expression regulation; humans; immunoblotting; lung neoplasms; neoplasm cells; sequence analysis
Abstract:
... Artemisia annua L. (A. annua) and its active components exhibit antitumour effects in many cancer cells. However, the biological processes and mechanisms involved are not well understood, especially for the treatment of non-small-cell lung cancer (NSCLC). This study aimed to comprehensively explore the biological processes of A. annua and its active components in NSCLC cells and to identify the me ...
DOI:
10.1016/j.phymed.2022.154095

29. Chemical characterization of non‐psychoactive Cannabis sativa L. extracts, in vitro antiproliferative activity and induction of apoptosis in chronic myelogenous leukaemia cancer cells

Author:
Lisa Anceschi; Alessandro Codeluppi; Virginia Brighenti; Riccardo Tassinari; Valentina Taglioli; Lucia Marchetti; Luca Roncati; Andrea Alessandrini; Lorenzo Corsi; Federica Pellati
Source:
Phytotherapy research 2022 v.36 no.2 pp. 914-927
ISSN:
0951-418X
Subject:
Cannabis sativa; apoptosis; cannabidiol; caspase-3; cell cycle; cell lines; cell proliferation; cytosol; dose response; flow cytometry; hemp; humans; leukemia; mass spectrometry; membrane potential; mitochondrial membrane; phytotherapy; research; ultra-performance liquid chromatography
Abstract:
... In this study, extracts from non‐psychoactive Cannabis sativa L. varieties were characterized by means of ultra high‐performance liquid chromatography coupled with high‐resolution mass spectrometry (UHPLC‐HRMS) and their antiproliferative activity was assessed in vitro. The human chronic myelogenous leukaemia cell line K562 was chosen to investigate the mechanism of cell death. The effect on the c ...
DOI:
10.1002/ptr.7357

30. Chikungunya Virus’ High Genomic Plasticity Enables Rapid Adaptation to Restrictive A549 Cells

Author:
Lien De Caluwé; Leo Heyndrickx; Sandra Coppens; Katleen Vereecken; Miguel E. Quiñones-Mateu; Andres Merits; Kevin K. Ariën; Koen Bartholomeeusen
Source:
Viruses 2022 v.14 no.2 pp. -
ISSN:
1999-4915
Subject:
Aedes aegypti; Aedes albopictus; Chikungunya virus; Flavivirus infections; cell lines; genomics; humans; lungs; mutation; phenotype; plasticity; viral genome; viruses
Abstract:
... Chikungunya virus (CHIKV) is an emerging arthropod-borne virus that has spread globally during the last two decades. The virus is mainly transmitted by Aedes aegypti and Aedes albopictus mosquitos and is thus capable of replicating in both human and mosquito cells. CHIKV has a broad tropism in vivo, capable of replicating in various tissues and cell types but largely excluding blood cells. This wa ...
DOI:
10.3390/v14020282

31. Comparative survival of environmental and clinical Mycobacterium abscessus isolates in a variety of diverse host cells

Author:
Charmie K. Vang; Stephanie N. Dawrs; Nicole M. Oberlag; Anah E. Gilmore; Nabeeh A. Hasan; Jennifer R. Honda
Source:
Journal of applied microbiology 2022 v.132 no.4 pp. 3302-3314
ISSN:
1364-5072
Subject:
Acanthamoeba; Arthrobacter; Mycobacterium abscessus; biofilm; cell lines; coculture; cytokines; epithelium; freshwater; humans; macrophages; microbiology; opportunistic pathogens; pathogenesis; soil; virulence
Abstract:
... AIMS: Mycobacterium abscessus subsp. abscessus (MABS) is an emerging, opportunistic pathogen found globally in freshwater biofilms and soil. Typically, isolates are treated as a uniform group of organisms and very little is known about their comparative survival in healthy host cells. We posit that environmentally‐ and clinically derived isolates, show differential infectivity in immune cells and ...
DOI:
10.1111/jam.15416

32. Construction of a telomerase-immortalized porcine tracheal epithelial cell model for swine-origin mycoplasma infection

Author:
Xing XIE; Fei HAO; Hai-yan WANG; Mao-da PANG; Yuan GAN; Bei-bei LIU; Lei ZHANG; Yan-na WEI; Rong CHEN; Zhen-zhen ZHANG; Wen-bin BAO; Yun BAI; Guo-qing SHAO; Qi-yan XIONG; Zhi-xin FENG
Source:
Journal of integrative agriculture 2022 v.21 no.2 pp. 504-520
ISSN:
2095-3119
Subject:
Mycoplasma hyopneumoniae; adhesion; agriculture; cell cycle; cell lines; color; epithelial cells; fluorescent antibody technique; humans; karyotyping; longevity; models; mucosa; mycoplasmosis; plasmids; quantitative analysis; quantitative polymerase chain reaction; respiratory system; swine; telomerase; virulence
Abstract:
... Primary porcine tracheal epithelial cells (PTECs) are an appropriate model for studying the molecular mechanism of various porcine respiratory diseases, including swine-origin mycoplasmas, which are isolated from respiratory tract of pigs and mainly found on the mucosal surface surrounding swine trachea. However, the short proliferation ability of primary PTECs greatly limits their lifespan. In th ...
DOI:
10.1016/S2095-3119(21)63644-4

33. Correlative N-glycan and charge variant analysis of cetuximab expressed in murine, chinese hamster and human expression systems

Author:
Anne Trappe; Florian Füssl; Silvia Millán-Martín; Rachel Ronan; Izabella Zaborowska; Jonathan Bones
Source:
Journal of chromatography 2022 v.1194 pp. 123186
ISSN:
1570-0232
Subject:
Cricetulus griseus; cation exchange chromatography; cell lines; glycosylation; humans; hydrophilicity; kidneys; mice; myeloma; polysaccharides; protein value; recombinant proteins; sialic acid; therapeutics
Abstract:
... A well-defined and controlled glycosylation pattern is important to maintain quality and safety of therapeutic proteins. Glycosylation is strongly dependent on the host cell line used for recombinant protein expression. Cetuximab, which is produced in mouse myeloma cells has been shown to harbour Fab glycans, which contain non-human like features and hence, can potentially cause an immunogenic res ...
DOI:
10.1016/j.jchromb.2022.123186

34. Cryopreservation of network bioactivity and multi-lineage stromal cell differentiation potential within three-dimensional synthetic hydrogels

Author:
Sudhir Khetan
Source:
Cryobiology 2022 v.105 pp. 41-49
ISSN:
0011-2240
Subject:
adipogenesis; bioactive properties; bone formation; cell lines; cell viability; cryobiology; cryopreservation; encapsulation; humans; hyaluronic acid; hydrogels; mesenchymal stromal cells; storage time
Abstract:
... Despite progress in many aspects of controlling cell behavior within synthetic three-dimensional hydrogels, approaches to cryopreserve these systems – encompassing the protection of both encapsulated cell viability and network bioactive functions – are lacking. Here, we demonstrate the retention of encapsulated human mesenchymal stromal cell (hMSC) viability following in situ cryopreservation rega ...
DOI:
10.1016/j.cryobiol.2021.12.003

35. Curcin C inhibit osteosarcoma cell line U2OS proliferation by ROS induced apoptosis, autophagy and cell cycle arrest through activating JNK signal pathway

Author:
Fei Wang; Peng Wu; Siying Qin; Yushan Deng; Pan Han; Xiao Li; Caixin Fan; Ying Xu
Source:
International journal of biological macromolecules 2022 v.195 pp. 433-439
ISSN:
0141-8130
Subject:
Jatropha curcas; antioxidant enzymes; apoptosis; autophagosomes; autophagy; cell cycle checkpoints; cell lines; cell proliferation; humans; membrane potential; mitochondrial membrane; neoplasm cells; osteosarcoma; ribosomes; signal transduction; toxicity
Abstract:
... Osteosarcoma is a kind of primary bone malignant tumors. Its cure rate has been stagnant in the past decade years. Curcin C belongs to type I ribosome inactivating proteins, extracted from the cotyledons of post-germinated Jatropha curcas seeds. It can inhibit the proliferation of several tumor lines including U2OS cells with extraordinary efficiency. The treated U2OS cells were arrested in both S ...
DOI:
10.1016/j.ijbiomac.2021.11.156

36. Curcumin induces apoptosis through caspase dependent pathway in human colon carcinoma cells

Author:
Gülçin Yavuz Türel; Nilüfer Şahin Calapoğlu; Dilek Bayram; Meltem Özgöçmen; Vehbi Atahan Toğay; Eda Evgen Tülüceoğlu
Source:
Molecular biology reports 2022 v.49 no.2 pp. 1351-1360
ISSN:
0301-4851
Subject:
apoptosis; bioavailability; caspase-3; cell lines; cell proliferation; colorectal neoplasms; curcumin; gene expression; genes; humans; immunohistochemistry; molecular biology; neoplasm cells
Abstract:
... BACKGROUND: We investigated the apoptotic effects of curcumin in the colon carcinoma cell line SW480. METHODS AND RESULTS: Cells were treated with 40–200 μM curcumin for 24, 48, and 72 h, and the IC₅₀ values were determined for each time interval. BrdU, caspase-3, and TUNEL staining results and the gene expression of FADD, CASP8, and CASP3 were evaluated. Curcumin treatments significantly inhibite ...
DOI:
10.1007/s11033-021-06965-y

37. Customized blood-brain barrier shuttle peptide to increase AAV9 vector crossing the BBB and augment transduction in the brain

Author:
Xintao Zhang; Zheng Chai; Amanda Lee Dobbins; Michelle S. Itano; Charles Askew; Zhe Miao; Hongqian Niu; R. Jude Samulski; Chengwen Li
Source:
Biomaterials 2022 v.281 pp. 121340
ISSN:
0142-9612
Subject:
Dependoparvovirus; bacteriophages; biocompatible materials; blood; blood-brain barrier; brain; capsid; cell lines; gene transfer; genetic engineering; humans; mice; models; neurons; peptides; phenotype; physiological transport; polysaccharides; therapeutics; toxicity
Abstract:
... Recombinant adeno-associated virus (rAAV) vectors have been widely used as favored delivery vehicles for the treatment of inherited diseases in clinical trials, including neurological diseases. However, the noninvasive systemic delivery of rAAV to the central nervous system is severely hampered by the blood-brain barrier (BBB). Several approaches have been exploited to enhance AAV vector brain tra ...
DOI:
10.1016/j.biomaterials.2021.121340

38. Cytotoxic, genotoxic, and carcinogenic effects of acrylamide on human lung cells

Author:
Seval Kontaş Yedier; Zülal Atlı Şekeroğlu; Vedat Şekeroğlu; Birsen Aydın
Source:
Food and chemical toxicology 2022 v.161 pp. 112852
ISSN:
0278-6915
Subject:
DNA; acrylamides; agar; carcinogenesis; carcinogenicity; cell lines; cell viability; comet assay; cytotoxicity; epithelial cells; fluorescent antibody technique; histones; humans; lung neoplasms; lungs; mutagens; risk; risk assessment
Abstract:
... While an association between acrylamide (AC) exposure and the risk of developing cancer has been shown in some studies, there are very limited data on the relationship between AC exposure and lung cancer risk. Thus, we investigated the cytotoxic, genotoxic, and carcinogenic effects of AC on human lung bronchial epithelial cell line (BEAS-2B cells). AC (5 and 10 mM) significantly decreased the cell ...
DOI:
10.1016/j.fct.2022.112852

39. Cytotoxicity and anti-HIV activities of extracts of the twigs of Croton dichogamus Pax

Author:
Ermias Mergia Terefe; Faith Apolot Okalebo; Solomon Derese; Gaber El-Saber Batiha; Amal Youssef; Mohammed Alorabi; Joseph Muriuki
Source:
BMC complementary medicine and therapies 2022 v.22 no.1 pp. 49
ISSN:
2662-7671
Subject:
Croton; Human immunodeficiency virus; acquired immunodeficiency syndrome; affordability; alternative medicine; antiretroviral agents; antiretroviral properties; cell culture; cell lines; cytotoxicity; drug resistance; hexane; humans; immunosuppression; methanol; methylene chloride; people; virus replication
Abstract:
... BACKGROUND: Acquired immunodeficiency syndrome (AIDS) is a clinical syndrome resulting from infection with human immunodeficiency virus (HIV), which causes profound immunosuppression. Anti-HIV drugs that are currently available are chemically synthesized and are frequently limited by side effects, the emergence of drug resistance, affordability, and availability, with over 5 million people in the ...
DOI:
10.1186/s12906-022-03532-1

40. Design and development of DSPC:DAP:PDMAEMA-b-PLMA nanostructures: from the adumbration of their morphological characteristics to in vitro evaluation

Author:
Natassa Pippa; Aleksander Forys; Hektor Katifelis; Varvara Chrysostomou; Barbara Trzebicka; Maria Gazouli; Costas Demetzos; Stergios Pispas
Source:
Colloids and surfaces 2022 v.632 pp. 127768
ISSN:
0927-7757
Subject:
antigens; cell lines; composite polymers; cytotoxicity; dose response; drugs; heat; humans; kidneys; phospholipids; transmission electron microscopy
Abstract:
... In this study, 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-dioleoyl-3-dimethylammonium-propane (DAP) and amphiphilic block copolymer poly(2-(dimethylamino)ethyl methacrylate)-b-poly(lauryl methacrylate) (PLMA-b-PDMAEMA) in the weight ratios: 1:0:0.03, 1:0.1:0.03, 1:0.3:0.03, 1:0.5:0.03, 1:0.7:0.03, and1:1:0.03, were used for the design and development of hybrid polymer-lipid nanostructu ...
DOI:
10.1016/j.colsurfa.2021.127768

41. Detection of volatile organic compounds from human prostate cancer cell using canine olfaction

Author:
Seongsoo Jeong; Dongwook Kim; Wun-Jae Kim; Gonhyung Kim
Source:
Journal of veterinary behavior 2022 v.49 pp. 80-84
ISSN:
1558-7878
Subject:
behavior; blood serum; cell lines; dogs; humans; hyperplasia; neoplasm cells; prostate-specific antigen; prostatic neoplasms; prostatitis; smell; urinary bladder neoplasms; volatile organic compounds
Abstract:
... Prostate cancer (PCa) is one of the most prevalent cancers of men worldwide. Currently, evaluating serum prostate-specific antigen, combined with digital rectal examination, is used as the diagnostic approach for early detection of PCa, but it has low sensitivity and difficulty in discriminating from non-malignant conditions such as prostatitis and benign prostate hyperplasia. As an alternative, a ...
DOI:
10.1016/j.jveb.2021.10.010

42. Development and assessment of a new bioassay for accurate quantification of Type I interferons induced by bovine respiratory viruses

Author:
Julie Lion; Gilles Meyer; Mariette Ducatez; Gilles Foucras; Adrien Lion
Source:
Journal of immunological methods 2022 v.504 pp. 113256
ISSN:
0022-1759
Subject:
bioassays; bovine respiratory disease; cell lines; cytokines; genes; humans; immunomodulation; industry; kidneys; luciferase
Abstract:
... Type I interferon (IFN-I) plays a major role in antiviral and inflammatory processes of the infected host. In the bovine industry, the bovine respiratory disease complex is a major cause of economic and health problems. This disease is caused by interactions of pathogens, together with environmental and host factors. Several pathogens have been identified as causal agents of respiratory diseases i ...
DOI:
10.1016/j.jim.2022.113256

43. Development of a CHO cell line for stable production of recombinant antibodies against human MMP9

Author:
Jina Ryu; Eun-Jung Kim; Joo-Kyung Kim; Tai Hyun Park; Byung-Gee Kim; Hee-Jin Jeong
Source:
BMC biotechnology 2022 v.22 no.1 pp. 8
ISSN:
1472-6750
Subject:
Cricetulus griseus; biomarkers; biotechnology; cell lines; gelatinase B; humans
Abstract:
... BACKGROUND: Human matrix metalloproteinase 9 (hMMP9) is a biomarker in several diseases, including cancer, and the need for developing detectors and inhibitors of hMMP9 is increasing. As an antibody against hMMP9 can be selectively bound to hMMP9, the use of anti-MMP9 antibody presents new possibilities to address hMMP9-related diseases. In this study, we aimed to establish a stable Chinese hamste ...
DOI:
10.1186/s12896-022-00738-6

44. Development of a three dimensional (3D) knitted scaffold for myocardial tissue engineering. Part II: biological performance of the knitted scaffolds

Author:
Derya Haroglu; Ahmet Eken; Zeynep Burçin Gönen; Dilek Bahar
Source:
The journal of the Textile Institute 2022 v.113 no.5 pp. 895-905
ISSN:
1754-2340
Subject:
adhesion; anisotropy; cell adhesion; cell lines; fabrics; fibronectins; humans; interleukin-11; medicine; mice; myoblasts; myocardial infarction; myocardium; polyethylene terephthalates; porosity; therapeutics
Abstract:
... Cardiac patch has been proposed as an alternative therapeutic tissue engineering strategy to treat damaged heart for patients who survive the acute myocardial infarction (MI). To that end, studies have focused on designing an ideal cardiac patch assisting myocardium to repair itself. The engineering of three dimensional (3 D) anisotropic knitted scaffolds could be an efficient and effective strate ...
DOI:
10.1080/00405000.2021.1907990

45. Development of an in vitro model for animal species susceptibility to SARS-CoV-2 replication based on expression of ACE2 and TMPRSS2 in avian cells

Author:
Darrell R. Kapczynski; Ryan Sweeney; Erica Spackman; Mary Pantin-Jackwood; David L. Suarez
Source:
Virology 2022 v.569 pp. 1-12
ISSN:
0042-6822
Subject:
Capra hircus; Hipposideros; Mesocricetus auratus; Myotis lucifugus; Severe acute respiratory syndrome coronavirus 2; birds; cats; cell culture; cell lines; fibroblasts; genetically modified organisms; goats; horses; humans; models; pandemic; peptidyl-dipeptidase A; serine proteinases; swine; virology; viruses
Abstract:
... The SARS-CoV-2 (SARS-CoV-2) virus has caused a worldwide pandemic because of the virus's ability to transmit efficiently human-to-human. A key determinant of infection is the attachment of the viral spike protein to the host receptor angiotensin-converting enzyme 2 (ACE2). Because of the presumed zoonotic origin of SARS-CoV-2, there is no practical way to assess the susceptibility of every species ...
DOI:
10.1016/j.virol.2022.01.014

46. Diadema setosum: isolation of bioactive secondary metabolites with cytotoxic activity toward human cervical cancer

Author:
Fahd M. Abdelkarem; Ezz-Eldin K. Desoky; Alaa M. Nafady; Ahmed E. Allam; Aldoushy Mahdy; Ahmed Ashour; Kuniyoshi Shimizu
Source:
Natural product research 2022 v.36 no.4 pp. 1118-1122
ISSN:
1478-6427
Subject:
antineoplastic activity; cell lines; chromatography; colorimetry; cytotoxicity; ethyl acetate; humans; neoplasm cells; nuclear magnetic resonance spectroscopy; research; secondary metabolites; uterine cervical neoplasms
Abstract:
... In our promising project toward discovery of secondary metabolites with potential anticancer activity against human cervical cancer, seven marine organisms were screened for their cytotoxic activity against HeLa cancer cell line using MTT colorimetric assay. The crude extract of the outer shell of Diadema setosum showed promising activity with 88.02% inhibition at a concentration 250 µg/ml. Chroma ...
DOI:
10.1080/14786419.2020.1855162

47. Endrin potentiates early-stage adipogenesis in 3T3-L1 cells by activating the mammalian target of rapamycin

Author:
Jo Woon Seok; Jae Yeo Park; Hyun Ki Park; Hyangkyu Lee
Source:
Life sciences 2022 v.288 pp. 120151
ISSN:
0024-3205
Subject:
adipocytes; adipogenesis; bisphenol A; cell lines; corticosterone; dose response; endocrine system; endrin; genes; humans; insulin; insulin resistance; lipogenesis; lipolysis; metabolic diseases; obesity; protein kinases; rapamycin; tolylfluanid; triacylglycerols
Abstract:
... Obesogens are a type of endocrine-disrupting chemicals (EDCs) that disrupt the human endocrine system, resulting in obesity and metabolic disease. Several obesogens, including bisphenol A, tolylfluanid, and some pesticides, have been identified and studied previously; however, the underlying molecular mechanisms by which obesogens interfere with adipogenesis and induce insulin resistance in adipoc ...
DOI:
10.1016/j.lfs.2021.120151

48. Engineering Chemotherapeutic-Augmented Calcium Phosphate Nanoparticles for Treatment of Intraperitoneal Disseminated Ovarian Cancer

Author:
Miaojuan Qiu; Junzong Chen; Xiuyu Huang; Binbin Li; Shiqiang Zhang; Peng Liu; Qiang Wang; Zhi Rong Qian; Yihang Pan; Yu Chen; Jing Zhao
Source:
ACS applied materials & interfaces 2022 v.14 no.19 pp. 21954-21965
ISSN:
1944-8252
Subject:
antineoplastic activity; apoptosis; calcium; calcium phosphates; cell lines; doxorubicin; drug therapy; endoplasmic reticulum stress; humans; intraperitoneal injection; mitochondria; models; mortality; nanomedicine; ovarian neoplasms; toxicity
Abstract:
... Ovarian cancer is a common gynecologic malignancy with a high fatality rate. Intraperitoneal chemotherapy has been proved as an efficient clinical treatment for disseminated ovarian cancer. However, there are limitations for conventional small molecule drugs to achieve an ideal therapeutic effect. Herein, a synergistic treatment for intraperitoneally disseminated ovarian cancer was achieved by Arg ...
DOI:
10.1021/acsami.2c02552

49. Essential role of a conserved aspartate for the enzymatic activity of plasmanylethanolamine desaturase

Author:
Ernst R. Werner; Monica L. Fernández-Quintero; Nicolas Hulo; Georg Golderer; Sabrina Sailer; Katharina Lackner; Gabriele Werner-Felmayer; Klaus R. Liedl; Katrin Watschinger
Source:
Cellular and molecular life sciences 2022 v.79 no.4 pp. 214
ISSN:
1420-682X
Subject:
alanine; amino acid sequences; aspartic acid; biosynthesis; brain; cell lines; enzyme activity; glycerophospholipids; histidine; humans; mice; models; mutation; oxidoreductases; phenylalanine; protein synthesis; recombinant proteins; transfection; transmembrane proteins
Abstract:
... Plasmalogens are an abundant class of glycerophospholipids in the mammalian body, with special occurrence in the brain and in immune cell membranes. Plasmanylethanolamine desaturase (PEDS1) is the final enzyme of plasmalogen biosynthesis, which introduces the characteristic 1-O-alk-1′-enyl double bond. The recent sequence identification of PEDS1 as transmembrane protein 189 showed that its protein ...
DOI:
10.1007/s00018-022-04238-w

50. Establishment and characterization of a sheep endometrial epithelial cell line

Author:
Guangdong Hu; Kexing Hao; Fang Ling; Meijun Song; Jing Wang; XinLi Gu
Source:
Biochemical and biophysical research communications 2022 v.603 pp. 63-68
ISSN:
0006-291X
Subject:
Western blotting; apoptosis; cell lines; cell proliferation; endometrium; epithelial cells; epithelium; genes; humans; interferons; longevity; models; plasmids; research; sheep; telomerase
Abstract:
... Endometrial epithelial cells play a significant role in the "dialogue" between the embryo and the mother, but in vitro studies to clarify this are hampered by the limited lifespan of primary cells. As such, it is necessary to develop an in vitro model to study endometrial function. Morphological analysis showed that the pEECs were homogeneous, formed characteristic cobblestone monolayers, and expr ...
DOI:
10.1016/j.bbrc.2022.03.017