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1. 2‐Arachidonoyl glycerol suppresses gastric emptying via the cannabinoid receptor 1‐cholecystokinin signaling pathway in mice

Author:
Keita Ochiai; Rina Hirooka; Masayoshi Sakaino; Shigeo Takeuchi; Tohru Hira
Source:
Lipids 2022 v.57 no.3 pp. 173-181
ISSN:
0024-4201
Subject:
absorption; acetaminophen; antagonists; cannabinoid receptors; cell lines; cholecystokinin; cholecystokinin receptors; glycerol; intraperitoneal injection; mice; phenolsulfonphthalein; secretion
Abstract:
... 2‐Monoacylglycerol (2‐MAG) is one of the digestion products of dietary lipids. We recently demonstrated that a 2‐MAG, 2‐arachidonoyl glycerol (2‐AG) potently stimulated cholecystokinin (CCK) secretion via cannabinoid receptor 1 (CB1) in a murine CCK‐producing cell line, STC‐1. CCK plays a crucial role in suppressing postprandial gastric emptying. To examine the effect of 2‐AG on gastric emptying, ...
DOI:
10.1002/lipd.12341

2. A blueprint from nature: miRNome comparison of plasma cells and CHO cells to optimize therapeutic antibody production

Author:
Nadja Raab; Nikolas Zeh; Patrick Schlossbauer; Sven Mathias; Benjamin Lindner; Anna Stadermann; Martin Gamer; Simon Fischer; Karlheinz Holzmann; René Handrick; Kerstin Otte
Source:
New biotechnology 2022 v.66 pp. 79-88
ISSN:
1871-6784
Subject:
Cricetulus griseus; antibody formation; biopharmaceuticals; bioprocessing; biotechnology; cell lines; fatty acid metabolism; gene expression; humans; industry; lysine; mice; microRNA; monoclonal antibodies; prediction; product quality; secretion; therapeutics; transcriptomics
Abstract:
... Chinese Hamster Ovary (CHO) cells are the most frequently used biopharmaceutical production hosts, although industry is presently suffering from their variable recombinant product quality, insufficient long-term stability and low productivity. Here, we present an effort to address overall cell line engineering by a novel bottom-up microRNA (miRNA) screening approach. miRNAs are small non-coding RN ...
DOI:
10.1016/j.nbt.2021.10.005

3. Cyanidin-3-galactoside ameliorates silica-induced pulmonary fibrosis by inhibiting fibroblast differentiation via Nrf2/p38/Akt/NOX4

Author:
Chuang Ma; Mingyue Lyu; Chilu Deng; Xia Liu; Yanmin Cui; Yixiao Shen; Xianjun Meng; Jin Zhao
Source:
Journal of functional foods 2022 v.92 pp. 105034
ISSN:
1756-4646
Subject:
Aronia melanocarpa; NAD(P)H oxidase (H2O2-forming); anthocyanins; antioxidants; cell lines; collagen; fibroblasts; functional foods; mitogen-activated protein kinase; non-specific serine/threonine protein kinase; oxidation; phosphorylation; pulmonary fibrosis; secretion; silica
Abstract:
... Aronia melanocarpa is rich in anthocyanins with many beneficial effects. Cyanidin-3-galactoside (C3G), the most abundant anthocyanin in Aronia melanocarpa was extracted and applied to silica particle-induced (SP-induced) pulmonary fibrosis mice and transforming growth factor-β-induced differentiation of NIH3T3 fibroblasts cell line to investigate the anti-fibrotic properties and underlying cellula ...
DOI:
10.1016/j.jff.2022.105034

4. Hypervirulent FAdV-4 infection induces activation of the NLRP3 inflammasome in chicken macrophages

Author:
Baiyu Wang; Huifang Guo; Qilong Qiao; Qing Huang; Panpan Yang; Congcong Song; Mingzhen Song; Zeng Wang; Yongtao Li; Yuhe Miao; Jun Zhao
Source:
Poultry science 2022 v.101 no.3 pp. 101695
ISSN:
0032-5791
Subject:
caspase-1; cell lines; chickens; etiological agents; inflammasomes; interleukin-1beta; macrophages; pathogenesis; potassium; poultry industry; secretion; serotypes
Abstract:
... Fowl adenovirus serotype 4 (FAdV-4) is the primary causative agent of hepatitis–hydropericardium syndrome (HHS) causing great economic losses to the world poultry industry. The exact factors responsible for the pathogenesis of hypervirulent FAdV-4 have not been completely elucidated. Hypervirulent FAdV-4 infection induces inflammatory damages in accompany with a high level of proinflammatory inter ...
DOI:
10.1016/j.psj.2021.101695

5. Protease Inhibitors from Plants as Therapeutic Agents- A Review

Author:
M. S. Cid-Gallegos; L. J. Corzo-Ríos; C. Jiménez-Martínez; X. M. Sánchez-Chino
Source:
Plant foods for human nutrition 2022 v.77 no.1 pp. 20-29
ISSN:
0921-9668
Subject:
anticarcinogenic activity; aspartic proteinases; breasts; cell lines; chymotrypsin; cysteine; elastase; human nutrition; humans; lung neoplasms; obesity; secretion; serine; therapeutics; trypsin
Abstract:
... Plant-based diets are a great source of protease inhibitors (PIs). Two of the most well-known families of PIs are Bowman-Birk inhibitors (BBI) and Kunitz-type inhibitors (KTI). The first group acts mainly on trypsin, chymotrypsin, and elastase; the second is on serine, cysteine, and aspartic proteases. PIs can retard or inhibit the catalytic action of enzymes; therefore, they are considered non-nu ...
DOI:
10.1007/s11130-022-00949-4

6. The anti-tumor effects of CT-26 derived exosomes enriched by MicroRNA-34a on murine model of colorectal cancer

Author:
Maryam Hosseini; Kaveh Baghaei; Mahsa Hajivalili; Mohammad Reza Zali; Masoumeh Ebtekar; Davar Amani
Source:
Life sciences 2022 v.290 pp. 120234
ISSN:
0024-3205
Subject:
T-lymphocytes; angiogenesis; animal models; cell lines; colorectal neoplasms; cytotoxicity; exosomes; genes; immune evasion; interleukin-17; lymph; microRNA; secretion; spleen; therapeutics; tumor necrosis factors
Abstract:
... As conventional therapeutics failed to provide satisfied outcomes against one of the most prevalent cancers, colorectal cancer (CRC), we purposed to implicate MicroRNA (miR)-34a, as a major tumor suppressor, to be delivered by tumor-derived exosomes (TEXs) and investigated its anti-tumor functions in-vivo. TEXs were isolated from CT-26 cell line and loaded with miR-34a mimic. Then, mice bearing CR ...
DOI:
10.1016/j.lfs.2021.120234

7. A lithium-doped surface inspires immunomodulatory functions for enhanced osteointegration through PI3K/AKT signaling axis regulation

Author:
Feng Peng; Longhai Qiu; Mengyu Yao; Lidan Liu; Yufeng Zheng; Shuilin Wu; Qingdong Ruan; Xuanyong Liu; Yu Zhang; Mei Li; Paul K. Chu
Source:
Biomaterials science 2021 v.9 no.24 pp. 8202-8220
ISSN:
2047-4849
Subject:
angiogenesis; biocompatible materials; bone formation; bone marrow; cell lines; culture media; cytokines; gene expression; histology; lithium; macrophages; mice; models; orthopedics; oxidation; phenotype; secretion; titanium
Abstract:
... The response of immune systems is crucial to the success of biomedical implants in vivo and in particular, orthopedic implants must possess appropriate immunomodulatory functions to allow sufficient osteointegration. In this work, lithium (Li) is incorporated into titanium (Ti) implants by plasma electrolytic oxidation to realize slow and sustained release of Li ions. In vitro cellular behaviors o ...
DOI:
10.1039/d1bm01075a

8. A newly established canine NK‐type cell line and its cytotoxic properties

Author:
Malgorzata Grudzien; Aleksandra Pawlak; Justyna Kutkowska; Ewa Ziolo; Edyta Wysokińska; Wojciech Hildebrand; Bożena Obmińska‐Mrukowicz; Leon Strzadala; Andrzej Rapak
Source:
Veterinary and comparative oncology 2021 v.19 no.3 pp. 567-577
ISSN:
1476-5810
Subject:
cell lines; cytotoxicity; dogs; immunophenotyping; interleukin-12; interleukin-15; interleukin-18; interleukin-2; interleukin-21; neoplasms; polymerase chain reaction; secretion
Abstract:
... We established a canine natural killer (NK)‐type cell line called CNK‐89 derived from a dog with NK cell neoplasia. Immunophenotyping analysis showed positive staining for CD5, CD8, CD45, CD56, CD79a and NKp46, while negative for CD3, CD4, CD14, CD20, CD21, CD34, Thy1, IgG, IgM and MHCII. Polymerase chain reaction analysis revealed the presence of CD56, NKG2D, NKp30, NKp44, NKp46 and perforin, but ...
DOI:
10.1111/vco.12695

9. An in vitro screening method for probiotics with antidepressant-like effect using the enterochromaffin cell model

Author:
Peijun Tian; Huiyue Zhu; Renying Zou; Qinming Kong; Mengshu Xu; Jianxin Zhao; Hao Zhang; Wei Chen; Gang Wang
Source:
Food & function 2021 v.12 no.2 pp. 646-655
ISSN:
2042-650X
Subject:
animals; biobased products; blood serum; cell lines; corticosterone; data collection; detection; forced swimming test; genes; models; principal component analysis; probiotics; secretion; sucrose; tryptophan; validity
Abstract:
... Certain probiotics can regulate the host's neurobehavioral function through the microbiota–gut–brain axis. However, screening these probiotics is mainly carried out in animal models, and is costly and inefficient. Herein, a putative enterochromaffin cell line (RIN14B) was used as an in vitro pre-screening model; 30 bacterial strains were tested for bacteria-stimulated tryptophan hydroxylase 1 gene ...
DOI:
10.1039/d0fo02307h

10. Calcium Dobesilate Modulates PKCδ-NADPH Oxidase- MAPK-NF-κB Signaling Pathway to Reduce CD14, TLR4, and MMP9 Expression during Monocyte-to-Macrophage Differentiation: Potential Therapeutic Implications for Atherosclerosis

Author:
Florence Njau; Hermann Haller
Source:
Antioxidants 2021 v.10 no.11 pp. -
ISSN:
2076-3921
Subject:
atherosclerosis; calcium; cell lines; drugs; humans; hydrogen peroxide; hyperglycemia; inflammation; macrophages; monocytes; oxidative stress; pathogenesis; phosphorylation; secretion; therapeutics
Abstract:
... Monocyte-to-macrophage differentiation results in the secretion of various inflammatory mediators and oxidative stress molecules necessary for atherosclerosis pathogenesis. Consequently, this differentiation represents a potential clinical target in atherosclerosis. Calcium dobesilate (CaD), an established vasoactive and angioprotective drug in experimental models of diabetic microvascular complic ...
DOI:
10.3390/antiox10111798

11. Dietary Postbiotics Reduce Cytotoxicity and Inflammation Induced by Crystalline Silica in an In Vitro RAW 264.7 Macrophage Model

Author:
Xue Du; Jessica Rodriguez; Josephine Wee
Source:
Foods 2022 v.11 no.6 pp. -
ISSN:
2304-8158
Subject:
Bifidobacterium animalis subsp. lactis; cell lines; culture media; cytotoxicity; inflammasomes; inflammation; lipopolysaccharides; macrophages; mice; models; probiotics; secretion; silica; toxic substances
Abstract:
... Crystalline silica (cSiO₂) particles are naturally existing environmental toxicants. Exposure to cSiO₂ could cause local or systemic inflammation and aggregate inflammation-associated diseases. Dietary postbiotics are reported to possess anti-inflammatory activities; however, their effects on cSiO₂-triggered inflammation are unknown. Here, we investigate the impact of postbiotics from Lacticaseiba ...
DOI:
10.3390/foods11060877

12. Differential Exoproteome and Biochemical Characterisation of Neoparamoeba perurans

Author:
Kerrie Ní Dhufaigh; Natasha Botwright; Eugene Dillon; Ian O’Connor; Eugene MacCarthy; Orla Slattery
Source:
Microorganisms 2021 v.9 no.6 pp. -
ISSN:
2076-2607
Subject:
Oncorhynchus mykiss; Paramoeba perurans; cell lines; coculture; cytotoxicity; databases; ectoparasites; enterobactin; enzyme activity; etiological agents; gene expression regulation; microorganisms; proteomics; secretion; serine proteinases; virulence
Abstract:
... Infection with the protozoan ectoparasite Neoparamoeba perurans, the causative agent of AGD, remains a global threat to salmonid farming. This study aimed to analyse the exoproteome of both an attenuated and virulent N. perurans isolate using proteomics and cytotoxicity testing. A disproportionate presence of proteins from the co-cultured microbiota of N. perurans was revealed on searching an amal ...
DOI:
10.3390/microorganisms9061258

13. Examination of Novel Immunomodulatory Effects of L-Sulforaphane

Author:
Nadia Mazarakis; Jeremy Anderson; Zheng Quan Toh; Rachel A. Higgins; Lien Anh Ha Do; Rodney B. Luwor; Kenneth J. Snibson; Tom C. Karagiannis; Paul V. Licciardi
Source:
Nutrients 2021 v.13 no.2 pp. -
ISSN:
2072-6643
Subject:
Brassicaceae; Toll-like receptors; adults; antioxidant activity; cell lines; cell structures; chemoattractants; chemokines; dendritic cells; drugs; humans; interleukins; isothiocyanates; lipopolysaccharides; monocytes; secretion
Abstract:
... The dietary isothiocyanate L-sulforaphane (LSF), derived from cruciferous vegetables, is reported to have several beneficial biological properties, including anti-inflammatory and immunomodulatory effects. However, there is limited data on how LSF modulates these effects in human immune cells. The present study was designed to investigate the immunomodulatory effects of LSF (10 µM and 50 µM) on pe ...
DOI:
10.3390/nu13020602

14. Heterologous expression of scFv fragment against Vairimorpha (Nosema) ceranae hexokinase in Sf9 cell culture inhibits microsporidia intracellular growth

Author:
Viacheslav V. Dolgikh; Vladimir S. Zhuravlyov; Igor V. Senderskiy; Anastasiya N. Ignatieva; Sergey A. Timofeev; Elena V. Seliverstova
Source:
Journal of invertebrate pathology 2022 v.191 pp. 107755
ISSN:
0022-2011
Subject:
Lepidoptera; Nosema; Vairimorpha; cell culture; cell lines; cytoplasm; heterologous gene expression; hexokinase; honey bees; invertebrates; parasites; reverse transcriptase polymerase chain reaction; secretion; spores
Abstract:
... Secretion of hexokinase (HK) by microsporidia into infected cells suggests an important role for this enzyme for the intracellular development of parasites. To verify whether the expression of HK-specific antibodies in the host cell cytoplasm can suppress the growth of microsporidia, we constructed an immune library of recombinant scFv fragments against the enzyme of the honey bee pathogen Vairimo ...
DOI:
10.1016/j.jip.2022.107755

15. Monocyte Infiltration and Differentiation in 3D Multicellular Spheroid Cancer Models

Author:
Natasha Helleberg Madsen; Boye Schnack Nielsen; Son Ly Nhat; Søren Skov; Monika Gad; Jesper Larsen
Source:
Pathogens 2021 v.10 no.8 pp. -
ISSN:
2076-0817
Subject:
cell lines; cytokines; fibroblasts; immunotherapy; macrophages; models; monocytes; neoplasm cells; neoplasm progression; neoplasms; phenotype; secretion
Abstract:
... Tumor-associated macrophages often correlate with tumor progression, and therapies targeting immune cells in tumors have emerged as promising treatments. To select effective therapies, we established an in vitro 3D multicellular spheroid model including cancer cells, fibroblasts, and monocytes. We analyzed monocyte infiltration and differentiation in spheroids generated from fibroblasts and either ...
DOI:
10.3390/pathogens10080969

16. NF-κB/ROS and ERK pathways regulate NLRP3 inflammasome activation in Listeria monocytogenes infected BV2 microglia cells

Author:
Lin Yuan; Yurong Zhu; Shuang Huang; Lin Lin; Xugan Jiang; Shengxia Chen
Source:
journal of microbiology 2021 v.59 no.8 pp. 771-781
ISSN:
1225-8873
Subject:
Listeria monocytogenes; animal models; cell lines; central nervous system; food pathogens; inflammasomes; microbiology; neuroglia; oligomerization; pyroptosis; secretion
Abstract:
... Listeria monocytogenes is a food-borne pathogen responsible for neurolisteriosis, which is potentially lethal in immunocompromised individuals. Microglia are the main target cells for L. monocytogenes in central nervous system (CNS). However, the precise mechanisms by which they trigger neuroinflammatory processes remain unknown. The BV2 microglial cell line and a murine model of L. monocytogenes ...
DOI:
10.1007/s12275-021-0692-9

17. Natural killer cells activity against multiple myeloma cells is modulated by osteoblast-induced IL-6 and IL-10 production

Author:
Christopher Uhl; Themba Nyirenda; David S. Siegel; Woo Y. Lee; Jenny Zilberberg
Source:
Heliyon 2022 v.8 no.3 pp. e09167
ISSN:
2405-8440
Subject:
antigens; bone marrow; cell lines; cell viability; coculture; cytotoxicity; drug resistance; humans; immunity; immunotherapy; interleukin-10; interleukin-6; myeloma; osteoblasts; secretion
Abstract:
... Natural killer (NK) cells are part of the innate arm of the immune system; as such NK cells can be activated rapidly to target virus-infected cells and tumor cells without prior sensitization. The human NK-92MI cell line is among the most widely used NK cell in preclinical research studies and has also been approved for clinical applications. Previous studies have shown that osteoblasts (OSB) conf ...
DOI:
10.1016/j.heliyon.2022.e09167

18. Quercetin and HSC70 coregulate the anti-inflammatory action of the ubiquitin-like protein MNSFβ

Author:
Morihiko Nakamura; Yuki Fukuma; Kaori Notsu; Megumi Kono
Source:
Molecular biology reports 2022 v.49 no.2 pp. 1213-1222
ISSN:
0301-4851
Subject:
Western blotting; anti-inflammatory activity; apoptosis; cell lines; chemokine CCL5; cytotoxicity; heat shock proteins; interferons; lipopolysaccharides; macrophages; molecular biology; necrosis; neoplasms; nitric oxide; quercetin; secretion
Abstract:
... BACKGROUND: Quercetin is a flavonol that modifies many cellular processes. Monoclonal nonspecific suppressor factor β is a member of the ubiquitin-like family of proteins that are involved in various biological processes. It has been demonstrated that quercetin regulates the effect of MNSFβ on tumor necrosis factor-α secretion in lipopolysaccharide (LPS)-stimulated macrophages. This study found th ...
DOI:
10.1007/s11033-021-06949-y

19. Single nucleotide polymorphisms in interleukin-6 attenuates hepatocytes injury in hypoxia/re-oxygenation via STAT3 signal pathway mediated autophagy

Author:
Hongwei Tang; Hongbo Fang; Wenzhi Guo; Shengli Cao; Danfeng Guo; Huapeng Zhang; Jie Gao; Shuijun Zhang
Source:
Molecular biology reports 2021 v.48 no.2 pp. 1687-1695
ISSN:
0301-4851
Subject:
apoptosis; autophagy; cell lines; cell viability; flow cytometry; genes; hepatocytes; humans; interleukin-6; liver; models; molecular biology; prognosis; protective effect; secretion; signal transduction; surgery
Abstract:
... Ischemia-reperfusion injury (IRI) is inevitable during liver surgery, and it is an important factor affecting the prognosis of patients. IL-6 rs1800796 single nucleotide polymorphisms (SNPs) can promote synthesis and secretion of IL-6 and protect hepatocytes from IRI. In this study, we investigated the mechanisms by which IL-6 alleviates hepatic IRI. We transfected lentivirus which carries IL-6 rs ...
DOI:
10.1007/s11033-020-06090-2

20. The Outer Membrane Vesicles of Salmonella enterica Serovar Typhimurium Activate Chicken Immune Cells through Lipopolysaccharides and Membrane Proteins

Author:
Hongxiao Cui; Yajun Sun; Hua Lin; Yan Zhao; Xin Zhao
Source:
Pathogens 2022 v.11 no.3 pp. -
ISSN:
2076-0817
Subject:
Salmonella Typhimurium; cell lines; chickens; inducible nitric oxide synthase; interleukin-6; lipopolysaccharides; macrophages; monocytes; pathogens; peptidase K; phagocytosis; polymyxin B; secretion; spleen
Abstract:
... Salmonella is a common pathogen which can secrete outer membrane vesicles (OMVs). However, the effect of OMVs from Salmonella enterica Serovar Typhimurium (S. Typhimurium) of poultry origin on cells of the chicken innate immune system is not well known. In this study, S. Typhimurium OMVs were first isolated from three different poultry strains of Salmonella, Salmonella CVCC542, SALA, and SALB. In ...
DOI:
10.3390/pathogens11030339

21. β-defensin 118 attenuates inflammation and injury of intestinal epithelial cells upon enterotoxigenic Escherichia coli challenge

Author:
Qingqing Fu; Qian Lin; Daiwen Chen; Bing Yu; Yuheng Luo; Ping Zheng; Xiangbing Mao; Zhiqing Huang; Jie Yu; Junqiu Luo; Hui Yan; Jun He
Source:
BMC veterinary research 2022 v.18 no.1 pp. 142
ISSN:
1746-6148
Subject:
anti-inflammatory agents; antimicrobial peptides; apoptosis; caspase-3; caspase-8; caspase-9; cell lines; cell viability; culture media; enterotoxigenic Escherichia coli; epithelial cells; immunomodulators; inflammation; interleukin-6; jejunum; necrosis; neoplasms; secretion; swine; transcription factor NF-kappa B; veterinary medicine
Abstract:
... BACKGROUND: Antimicrobial peptides including various defensins have been attracting considerable research interest worldwide, as they have potential to substitute for antibiotics. Moreover, AMPs also have immunomodulatory activity. In this study, we explored the role and its potential mechanisms of β-defensin 118 (DEFB118) in alleviating inflammation and injury of IPEC-J2 cells (porcine jejunum ep ...
DOI:
10.1186/s12917-022-03242-3

22. A Simple and Cost-Effective DNA Preparation Method Suitable for High-Throughput PCR Quantification of Hepatitis B Virus Genomes

Author:
Eunji Jo; Jaewon Yang; Alexander Koenig; Seung Kew Yoon; Marc P. Windisch
Source:
Viruses 2020 v.12 no.9 pp. -
ISSN:
1999-4915
Subject:
Hepatitis B virus; RNA; analytical kits; blood serum; cell lines; centrifugation; circular DNA; cost effectiveness; detergents; genome; heat inactivation; lamivudine; median effective concentration; nucleocapsid; patients; peptidase K; quantitative analysis; quantitative polymerase chain reaction; secretion; transcription (genetics); virion; wastes
Abstract:
... Hepatitis B virus (HBV) is a para-retrovirus that reverse transcribes its pregenomic RNA into relaxed circular DNA inside viral nucleocapsids. The number of HBV genomes produced in vitro is typically quantified using commercial silica-membrane-based nucleic acid purification kits to isolate total DNA followed by HBV-specific quantitative PCR (qPCR). However, despite the convenience of commercial k ...
DOI:
10.3390/v12090928

23. Analysis of genes encoding for proteolytic enzymes and cytotoxic proteins as virulence factors of Piscirickettsia salmonis in SHK‐1 cells

Author:
Jaime Figueroa; Daniela Villagrán; Carlos Cartes; Camila Solis; Guillermo Nourdin‐Galindo; Denise Haussmann
Source:
Journal of fish diseases 2021 v.44 no.5 pp. 495-504
ISSN:
0140-7775
Subject:
Piscirickettsia salmonis; bioinformatics; cell lines; cytotoxicity; enzyme activity; etiological agents; gene expression; hemolysins; mortality; muscles; proteolysis; salmon; secretion; thermolysin; virulence; Chile
Abstract:
... Piscirickettsia salmonis is the causative agent of Piscirickettsiosis, a systemic disease generating high mortality rates in farmed salmon cultures of southern Chile. Proteolytic enzymes are important virulence factors since they play a key role in bacterial invasion and proliferation within the host. Bacteria growing in muscle tissues are known to secrete proteases, but no proteolytic enzymes hav ...
DOI:
10.1111/jfd.13333

24. Anti-inflammatory effect of lavender (Lavandula angustifolia Mill.) essential oil prepared during different plant phenophases on THP-1 macrophages

Author:
Edina Pandur; Alex Balatinácz; Giuseppe Micalizzi; Luigi Mondello; Adrienn Horváth; Katalin Sipos; Györgyi Horváth
Source:
BMC complementary medicine and therapies 2021 v.21 no.1 pp. 287
ISSN:
2662-7671
Subject:
Gram-negative bacteria; Lavandula angustifolia; Pseudomonas aeruginosa; alternative medicine; anti-inflammatory activity; cell culture; cell lines; cross infection; essential oils; gene expression; humans; inflammation; interleukin-6; interleukin-8; linalool; luteolin; macrophages; models; monocytes; oils; quantitative polymerase chain reaction; secretion; Hungary
Abstract:
... BACKGROUND: Pseudomonas aeruginosa is the most common Gram-negative bacterium associated with nosocomial respiratory infections. Lavender essential oil is mainly used in aromatherapy, but it has several pharmacological and therapeutic properties. Furthermore, it possesses antifungal and antibacterial activities. The anti-inflammatory activity of essential oils may depend on the composition and the ...
DOI:
10.1186/s12906-021-03461-5

25. Bioreactivity, Guttation and Agents Influencing Surface Tension of Water Emitted by Actively Growing Indoor Mould Isolates

Author:
Maria A. Andersson; Johanna Salo; Orsolya Kedves; László Kredics; Irina Druzhinina; Jarek Kurnitski; Heidi Salonen
Source:
Microorganisms 2020 v.8 no.12 pp. -
ISSN:
2076-2607
Subject:
Aspergillus versicolor; Chaetomium globosum; Penicillium expansum; Rhizopus; Stachybotrys; Trichoderma atroviride; air; biochemical pathways; biomass; cell lines; conidia; droplets; fungal growth; guttation; hyphae; liquids; mammals; metabolites; microorganisms; secretion; staining; strains; surface tension; surfactants; toxicity; viability; water; weight
Abstract:
... The secretion of metabolites in guttation droplets by indoor moulds is not well documented. This study demonstrates the guttation of metabolites by actively growing common indoor moulds. Old and fresh biomasses of indoor isolates of Aspergillus versicolor, Chaetomium globosum, Penicillium expansum, Trichoderma atroviride, T. trixiae, Rhizopus sp. and Stachybotrys sp. were compared. Metabolic activ ...
DOI:
10.3390/microorganisms8121940

26. Cortactin Promotes Effective AGS Cell Scattering by Helicobacter pylori CagA, but Not Cellular Vacuolization and Apoptosis Induced by the Vacuolating Cytotoxin VacA

Author:
Irshad Sharafutdinov; Jakob Knorr; Delara Soltan Esmaeili; Steffen Backert; Nicole Tegtmeyer
Source:
Pathogens 2021 v.11 no.1 pp. -
ISSN:
2076-0817
Subject:
CRISPR-Cas systems; Helicobacter pylori; apoptosis; cell lines; cell movement; cytoskeleton; cytotoxins; eukaryotic cells; microfilament proteins; mutants; neoplasm cells; pathogens; phosphorylation; secretion; travel; virulence
Abstract:
... Cortactin is an actin-binding protein and actin-nucleation promoting factor regulating cytoskeletal rearrangements in eukaryotes. Helicobacter pylori is a gastric pathogen that exploits cortactin to its own benefit. During infection of gastric epithelial cells, H. pylori hijacks multiple cellular signaling pathways, leading to the disruption of key cell functions. Two bacterial virulence factors p ...
DOI:
10.3390/pathogens11010003

27. Diet-Derived Gallated Catechins Prevent TGF-β-Mediated Epithelial-Mesenchymal Transition, Cell Migration and Vasculogenic Mimicry in Chemosensitive ES-2 Ovarian Cancer Cells

Author:
Audrey-Ann Sicard; Tiziana Dao; Narjara Gonzalez Suarez; Borhane Annabi
Source:
Nutrition and cancer 2021 v.73 no.1 pp. 169-180
ISSN:
1532-7914
Subject:
biomarkers; cell lines; cell movement; chemoprevention; drug design; epigallocatechin gallate; fibronectins; flavanols; gelatinase A; genes; metastasis; moieties; neoplasm cells; nutrition; ovarian neoplasms; phenotype; phosphorylation; protein synthesis; secretion
Abstract:
... Background: Transforming growth factor (TGF)-β triggers ovarian cancer metastasis through epithelial-mesenchymal transition (EMT). Whereas drug design strategies targeting the TGF-β signaling pathway have been envisioned, the anti-TGF structure:function aspect of chemopreventive diet-derived catechins remains unexplored. Aim: We assessed the effects of eight catechins on TGF-β-mediated cell migrat ...
DOI:
10.1080/01635581.2020.1733624

28. Effects of Gastrodin on BV2 cells under oxygen-glucose deprivation and its mechanism

Author:
Ying Lv; Han Cao; Liangzhao Chu; Han Peng; Xiangchun Shen; Hua Yang
Source:
Gene 2021 v.766 pp. 145152
ISSN:
0378-1119
Subject:
Western blotting; apoptosis; cell lines; cerebrovascular disorders; cytokines; detection; drugs; enzyme-linked immunosorbent assay; flow cytometry; fluorescence; genes; human health; hypoxia; inflammation; ischemia; mechanism of action; models; mortality; neuroglia; oxygen; protective effect; proteins; secretion; signal transduction; toxicity; toxicity testing
Abstract:
... Cerebrovascular disease is one of the major diseases that seriously harm human health currently. The purpose of this study is to find an effective treatment and clarify its mechanism of action to provide a new idea and drug target for the clinical treatment of ischemic cerebrovascular disease.The microglia cell line (BV2 cell line) was cultured in vitro. Prepare a hypoxia ischemia cell model by OG ...
DOI:
10.1016/j.gene.2020.145152

29. Effects of a Potential Probiotic Strain Lactobacillus gasseri ATCC 33323 on Helicobacter pylori-Induced Inflammatory Response and Gene Expression in Coinfected Gastric Epithelial Cells

Author:
Mahdieh Yarmohammadi; Abbas Yadegar; Maryam Tajabadi Ebrahimi; Mohammad Reza Zali
Source:
Probiotics and antimicrobial proteins 2021 v.13 no.3 pp. 751-764
ISSN:
1867-1306
Subject:
Helicobacter pylori; Lactobacillus gasseri; adenocarcinoma; bacteria; cell lines; enzyme-linked immunosorbent assay; gene expression; humans; inflammation; integrins; interleukin-8; mixed infection; probiotics; secretion
Abstract:
... In the present study, we aimed to investigate the modulatory effects of a potential probiotic bacterium Lactobacillus gasseri ATCC 33323 on Helicobacter pylori-induced inflammatory response and gene expression in human gastric adenocarcinoma (AGS) cell line. The gastric epithelial cells were coinfected with a collection of H. pylori clinical strains alone or in combination with L. gasseri at a mul ...
DOI:
10.1007/s12602-020-09721-z

30. Ethyl Pyruvate Attenuates Microglial NLRP3 Inflammasome Activation via Inhibition of HMGB1/NF-κB/miR-223 Signaling

Author:
Melis Olcum; Kemal Ugur Tufekci; Devrim Yagmur Durur; Bora Tastan; Irem Nur Gokbayrak; Kursad Genc; Sermin Genc
Source:
Antioxidants 2021 v.10 no.5 pp. -
ISSN:
2076-3921
Subject:
caspase-1; cell lines; inflammasomes; interleukin-18; microRNA; mitochondria; neuroglia; nucleoproteins; pyroptosis; pyruvic acid; secretion
Abstract:
... Ethyl pyruvate is a molecule with anti-inflammatory and pro-metabolic effects. Ethyl pyruvate has been shown to ameliorate the clinical and pathological findings of neurodegenerative diseases such as Alzheimer’s and Parkinson’s Diseases in rodents. Its anti-inflammatory and neuroprotective effects are widely investigated in animal and cellular models. Our study aimed to investigate the mechanism o ...
DOI:
10.3390/antiox10050745

31. Exopolysaccharides of Bacillus amyloliquefaciens modulate glycemic level in mice and promote glucose uptake of cells through the activation of Akt

Author:
Yo-Chia Chen; Shen-Da Huang; Jing-Hong Tu; Jyun-Sian Yu; Annisa Oktafianti Nurlatifah; Wen-Chin Chiu; Yu-Han Su; Hsuan-Lun Chang; Diwyacitta Antya Putri; Hsueh-Ling Cheng
Source:
International journal of biological macromolecules 2020 v.146 pp. 202-211
ISSN:
0141-8130
Subject:
AMP-activated protein kinase; Bacillus amyloliquefaciens; blood glucose; cell lines; epithelial cells; exopolysaccharides; galactose; glucagon-like peptide 1; glucose; glucose tolerance tests; glycemic control; glycemic effect; hepatocytes; high performance liquid chromatography; insulin receptor substrate proteins; intestinal mucosa; intraperitoneal injection; mannose; mice; phosphatidylinositol 3-kinase; polymers; probiotics; secretion; soil
Abstract:
... Bacillus amyloliquefaciens is a probiotic for animals. A strain of B. amyloliquefaciens designated amy-1 was isolated from soil, and the exopolysaccharides (EPSs) of the strain were characterized in terms of their effect on glycemic control. The EPSs were composed of mannose, glucose, and galactose, with the major components being polymers larger than 1000 kDa as revealed by size-exclusion high-pe ...
DOI:
10.1016/j.ijbiomac.2019.12.217

32. High-Throughput Single-Cell Extracellular Vesicle Secretion Analysis on a Desktop Scanner without Cell Counting

Author:
Fengjiao Zhu; Yahui Ji; Linmei Li; Xue Bai; Xianming Liu; Yong Luo; Tingjiao Liu; Bingcheng Lin; Yao Lu
Source:
Analytical chemistry 2021 v.93 no.39 pp. 13152-13160
ISSN:
1520-6882
Subject:
Poisson distribution; biomedical research; cell lines; fluorescent antibody technique; gold; human health; humans; neoplasm cells; scanners; secretion; silver; squamous cell carcinoma; titration
Abstract:
... Single-cell EV (extracellular vesicle) secretion analysis is emerging for a better understanding of non-genetic cellular heterogeneity regulating human health and diseases through intercellular mediators. However, the requirements of expensive and bulky instrumentations hinder its widespread use. Herein, by combining gold nanoparticle-enhanced silver staining and the Poisson distribution, we repor ...
DOI:
10.1021/acs.analchem.1c01446

33. Humanized mediator release assay as a read-out for allergen potency

Author:
Wenger Mario; Bethanis Athanasios; Johnson Litty; Aglas Lorenz
Source:
Journal of visualized experiments 2021 no.172 pp. e62702
ISSN:
1940-087X
Subject:
allergenicity; allergens; cell lines; complement; crosslinking; histamine; humans; immunoglobulin E; immunotherapy; ligands; neoplasm cells; plasma membrane; pollen; rats; secretion
Abstract:
... Mediator release assays analyze in vitro immunoglobulin E (IgE)-mediated degranulation and secretion of mediators by effector cells, such as mast cells and basophils, upon stimulation with serial dilutions of putative allergens. Therefore, these assays represent an essential tool that mimics the in vivo degranulation process, which occurs upon allergen exposure in sensitized patients or in skin pr ...
DOI:
10.3791/62702

34. Induction of ZCCHC6 expression in peripheral blood mononuclear cells by HNSCC secretions

Author:
Areeya Arayataweegool; Ratakorn Srisuttee; Fardeela Bin-Alee; Patnarin Mahattanasakul; Napadon Tangjaturonrasme; Virachai Kerekhanjanarong; Apiwat Mutirangura; Nakarin Kitkumthorn
Source:
Gene 2020 v.754 pp. 144880
ISSN:
0378-1119
Subject:
cell lines; coculture; gene expression; genes; head; models; mononuclear leukocytes; neck; patients; quantitative polymerase chain reaction; screening; secretion; squamous cell carcinoma; transcriptomics; zinc finger motif
Abstract:
... Cancer secretion can change the properties of adjacent cells, including peripheral blood mononuclear cells (PBMCs). We investigated whether such secretion influences messenger RNA expression in PBMCs of patients with head and neck squamous cell carcinoma (HNSCC). In the present study, co-culture model of normal PBMCs and HNSCC cell lines were established. The PBMCs were subsequently subjected to R ...
DOI:
10.1016/j.gene.2020.144880

35. L- theanine relieves acute alcoholic liver injury by regulating the TNF-α/NF-κB signaling pathway in C57BL/6J mice

Author:
Lingli Sun; Shuai Wen; Qiuhua Li; Xingfei Lai; Ruohong Chen; Zhenbiao Zhang; Dongli Li; Shili Sun
Source:
Journal of functional foods 2021 v.86 pp. 104699
ISSN:
1756-4646
Subject:
antioxidant activity; cell lines; ethanol; fibrosis; hepatoprotective effect; inflammation; interleukin-6; liver; secretion
Abstract:
... Acute alcoholic liver injury (AALI) refers to the inflammatory hepatic damage caused by alcohol. In severe cases, it can lead to steatohepatitis, alcoholic fibrosis and even cirrhosis. In this study, we analyzed the hepatoprotective activity of L-theanine on mice fed with a large amount of ethanol at one time, and the underlying molecular mechanism. The extensive liver injury caused by alcohol fee ...
DOI:
10.1016/j.jff.2021.104699

36. Notopterygium incisum extract (NRE) rescues cognitive deficits in APP/PS1 Alzhneimer's disease mice by attenuating amyloid-beta, tau, and neuroinflammation pathology

Author:
Xiao-wen Jiang; Wen-wu Liu; Yu-ting Wu; Qiong Wu; Hong-yuan Lu; Zi-hua Xu; Hui-yuan Gao; Qing-chun Zhao
Source:
Journal of ethnopharmacology 2020 v.249 pp. 112433
ISSN:
0378-8741
Subject:
Alzheimer disease; Hansenia weberbaueriana; Lentivirus; Oriental traditional medicine; Western blotting; cell lines; cognition; cognitive disorders; dose response; elderly; enzyme inhibitors; enzyme-linked immunosorbent assay; hippocampus; interleukin-1beta; kidneys; liver; mice; models; paralysis; plant extracts; protein kinase C; psoralens; secretion; tau-protein kinase; tumor necrosis factor-alpha
Abstract:
... Alzheimer's disease (AD) is a frequently occurring disease of the elderly, and “deficiency” is the root of AD. Most famous experts of traditional Chinese medicine believe that the disease is based on deficiency, and the deficiency of kidney essence is the basis. Notopterygium incisum (Qiang huo) is beneficial to bladder, liver, and kidneys. It is used to treat liver and kidney deficiency, language ...
DOI:
10.1016/j.jep.2019.112433

37. On chip detection of glial cell-derived neurotrophic factor secreted from dopaminergic cells under magnetic stimulation

Author:
Renyuan Yang; Joseph Boldrey; David Jiles; Ian Schneider; Long Que
Source:
Biosensors & bioelectronics 2021 v.182 pp. 113179
ISSN:
0956-5663
Subject:
Parkinson disease; biosensors; cell lines; films (materials); magnetism; models; nanopores; rats; secretion; transcranial magnetic stimulation
Abstract:
... Glial cell-derived neurotrophic factor (GDNF) is a small protein potently promoting the survival of dopaminergic and motor neurons. GDNF can be secreted from different types of cells including the dopaminergic neural cell line, N27. N27 cells, a rat dopaminergic neural cell line, is regarded as a suitable in vitro model for Parkinson's disease (PD) research. For PD treatment, transcranial magnetic ...
DOI:
10.1016/j.bios.2021.113179

38. Overexpression of transcription factor BLIMP1/prdm1 leads to growth inhibition and enhanced secretory capacity in Chinese hamster ovary cells

Author:
Mauro Torres; Alan J. Dickson
Source:
Metabolic engineering 2021 v.67 pp. 237-249
ISSN:
1096-7176
Subject:
Cricetulus griseus; biopharmaceuticals; cell growth; cell lines; energy; growth retardation; immunoglobulin G; manufacturing; phenotype; protein synthesis; recombinant proteins; secretion; transcription factors
Abstract:
... Chinese hamster ovary (CHO) cells present inherent limitations for processing and secretion of large amounts of recombinant proteins, especially for those requiring complex post-translational processing. To tackle these limitations, we engineered CHO host cells (CHOK1 and CHOS) to overexpress the transcription factor BLIMP1/prdm1 (a master regulator of the highly-secreting phenotype of antibody-pr ...
DOI:
10.1016/j.ymben.2021.07.004

39. Poly (I: C) inhibits reticuloendothelial virus replication in chicken macrophage-like cells through the activation of toll-like receptor-3 signaling

Author:
Yu Bai; Xinhua Cui; Xueli Gao; Chaonan Liu; Xiaoping Lv; Shimin Zheng
Source:
Molecular immunology 2021 v.136 pp. 110-117
ISSN:
0161-5890
Subject:
Toll-like receptor 3; cell lines; chickens; immunosuppression; polyinosinic-polycytidylic acid; poultry industry; secretion; therapeutics; virus replication; viruses
Abstract:
... Reticuloendothelial virus (REV) is widely found in many domestic poultry areas and results in severe immunosuppression of infected chickens. This increases the susceptibility to other pathogens, which causes economic losses to the poultry industry. The aim of our study was to determine whether polyinosinic-polycytidylic acid [Poly (I: C)] treatment could inhibit REV replication in chicken macropha ...
DOI:
10.1016/j.molimm.2021.05.013

40. Porcine placenta extract improves high-glucose-induced angiogenesis impairment

Author:
Chatchai Nensat; Worawat Songjang; Rutaiwan Tohtong; Tuangporn Suthiphongchai; Suchada Phimsen; Panthip Rattanasinganchan; Pornphimon Metheenukul; Sarawut Kumphune; Arunya Jiraviriyakul
Source:
BMC complementary medicine and therapies 2021 v.21 no.1 pp. 66
ISSN:
2662-7671
Subject:
Western blotting; alternative medicine; amputation; angiogenesis; apoptosis; caspase-3; cell lines; diabetes; endothelial cells; enzyme-linked immunosorbent assay; glucose; placenta; reactive oxygen species; secretion; swine; toxicity testing; viability
Abstract:
... BACKGROUND: High glucose (HG)-induced reactive oxygen species (ROS) overproduction impairs angiogenesis that is one pivotal factor of wound healing process. Angiogenesis impairment induces delayed wound healing, whereby it eventually leads to amputation in cases of poorly controlled diabetes with diabetic ulceration. Porcine placenta extract (PPE) is a natural waste product that comprises plenty o ...
DOI:
10.1186/s12906-021-03243-z

41. Pyrrolizidine Alkaloids Disturb Bile Acid Homeostasis in the Human Hepatoma Cell Line HepaRG

Author:
Julia Waizenegger; Josephin Glück; Marcus Henricsson; Claudia Luckert; Albert Braeuning; Stefanie Hessel-Pras
Source:
Foods 2021 v.10 no.1 pp. -
ISSN:
2304-8158
Subject:
bile acids; cell lines; enzymes; foods; genes; hepatoma; hepatotoxicity; homeostasis; humans; lead; liver; metabolites; pyrrolizidine alkaloids; secretion; synthesis; transcription factors; transporters
Abstract:
... 1,2-unsaturated pyrrolizidine alkaloids (PAs) belong to a group of secondary plant metabolites. Exposure to PA-contaminated feed and food may cause severe hepatotoxicity. A pathway possibly involved in PA toxicity is the disturbance of bile acid homeostasis. Therefore, in this study, the influence of four structurally different PAs on bile acid homeostasis was investigated after single (24 h) and ...
DOI:
10.3390/foods10010161

42. Quorum quenchers affect the virulence regulation of non-mucoid, mucoid and heavily mucoid biofilms co-cultured on cell lines

Author:
Rachith Kalgudi; Roya Tamimi; Godfrey Kyazze; Tajalli Keshavarz
Source:
Applied microbiology and biotechnology 2021 v.105 no.23 pp. 8853-8868
ISSN:
0175-7598
Subject:
Pseudomonas aeruginosa; alginates; biofilm; biotechnology; cell lines; coculture; environmental DNA; enzyme-linked immunosorbent assay; epithelial cells; epithelium; farnesol; gene expression; interleukin-8; keratinocytes; lungs; pyocyanin; rhamnolipids; secretion; virulence
Abstract:
... Biofilm formation conferring pathogenicity is a survival strategy for Pseudomonas aeruginosa. P. aeruginosa’s virulence may differ due to differences in host-microbe interactions and the growth environment. The epithelial cell line within the respiratory system and the keratinocytes on the skin form the first physical barrier of defence. P. aeruginosa spp. biofilm formation and virulence factor se ...
DOI:
10.1007/s00253-021-11638-8

43. Rhipicephalus sanguineus salivary gland extract as a source of immunomodulatory molecules

Author:
Melissa Carolina Pereira; Elen Fernanda Nodari; Marina Rodrigues de Abreu; Lisiery Negrini Paiatto; Patrícia Ucelli Simioni; Maria Izabel Camargo-Mathias
Source:
Experimental & applied acarology 2021 v.83 no.3 pp. 387-398
ISSN:
0168-8162
Subject:
Rhipicephalus sanguineus; acarology; anticoagulants; apoptosis; cell lines; cytotoxicity; dogs; females; immunomodulation; inflammation; lipopolysaccharides; macrophage activation; nitric oxide; saliva; salivary glands; secretion; ticks
Abstract:
... Rhipicephalus sanguineus sensu lato (s.l.), popularly known as ‘brown dog tick’, is the primary vector of pathogens affecting dogs worldwide. To enter the host’s organism, these pathogens utilise the anticoagulant, antiplatelet, anti-inflammatory and immunomodulatory actions of compounds present in the tick’s saliva; such compounds are released by the ectoparasite in order to attach and feed on do ...
DOI:
10.1007/s10493-021-00591-w

44. Screening of Anti-Inflammatory Components of Qin Jin Hua Tan Tang by a Multivariate Statistical Analysis Approach for Spectrum-Effect Relationships

Author:
Feipeng Duan; Yisheng Li; Meizhen Zhao; Tianyong Hu; Xinquan Pan; Yue Feng; Fang Ma; Shuqi Qiu; Yiqing Zheng
Source:
Journal of Analytical Methods in Chemistry (Online) 2021 v.2021 pp. -
ISSN:
2090-8873
Subject:
active ingredients; anti-inflammatory activity; baicalin; butanol; cell lines; ethanol; ethyl acetate; inflammation; macrophages; mice; multivariate analysis; respiratory tract diseases; secretion; therapeutics; ultra-performance liquid chromatography
Abstract:
... Qing Jin Hua Tan Tang (QJHTT) exerts therapeutic effects in patients with chronic obstructive pulmonary disease (COPD) by alleviating inflammation. However, the anti-inflammatory components of QJHTT have not yet been reported. Our study aimed to screen the active anti-inflammatory components of QJHTT using a multivariate statistical analysis approach for spectrum-effect relationships. Different po ...
DOI:
10.1155/2021/6348979

45. Staphylococcal enterotoxin M induced inflammation and impairment of bovine mammary epithelial cells

Author:
Yanying Zhao; Junni Tang; Danru Yang; Cheng Tang; Juan Chen
Source:
Journal of dairy science 2020 v.103 no.9 pp. 8350-8359
ISSN:
0022-0302
Subject:
IKappaB kinase; Staphylococcus aureus; Western blotting; adenosine triphosphate; adhesion; analytical kits; apoptosis; autophagy; bovine mastitis; caspase-3; cattle; cell adhesion; cell lines; cell viability; chemokine CCL2; enterotoxins; enzyme-linked immunosorbent assay; epithelial cells; epithelium; fluorescent dyes; genes; inflammation; intercellular adhesion molecule-1; interleukin-6; iodides; isothiocyanates; lactate dehydrogenase; mammary glands; membrane potential; mitochondrial membrane; necrosis; oxidative stress; pathogenesis; pathogens; phosphorylation; propidium; rapamycin; reactive oxygen species; research and development; secretion; staining; transcription factor NF-kappa B; tumor necrosis factor-alpha; virulence; United Kingdom
Abstract:
... Staphylococcus aureus is one of the major etiological pathogens of bovine mastitis. Its invasion into mammary epithelial cells has been proven to be a key event in the pathogenesis of mastitis. However, the specific pathogenic factors have not been clearly identified. Staphylococcus aureus often triggers infections by releasing virulence factor. Recent several studies reported that staphylococcal ...
DOI:
10.3168/jds.2019-17444

46. The surface-dependent biological effect of protein-gold nanoclusters on human immune system mimetic cells

Author:
Agnieszka Czyżowska; Anna Barbasz; Lilianna Szyk-Warszyńska; Magdalena Oćwieja; Edit Csapó; Ditta Ungor
Source:
Colloids and surfaces 2021 v.620 pp. 126569
ISSN:
0927-7757
Subject:
apoptosis; blood serum; cattle; cell lines; comparative study; confocal microscopy; globulins; humans; immune system; leukemia; lysozyme; mitochondria; nanogold; nanohybrids; oxidative stress; secretion; toxicity
Abstract:
... In this paper, the comprehensive toxicity mechanism of four well-defined protein-stabilized gold nanoclusters (Au NCs) is firstly highlighted against promyelocytic leukaemia cells (HL-60). For this purpose, bovine serum albumins (BSA) and human serum albumins (HSA), as well as lysozyme (LYZ) and gamma globulin (γG) immunoproteins were applied as reducing and stabilizing agents of the Au nanohybrid ...
DOI:
10.1016/j.colsurfa.2021.126569

47. Validation of prostate cancer risk variants rs10993994 and rs7098889 by CRISPR/Cas9 mediated genome editing

Author:
Xing Wang; James E. Hayes; Xing Xu; Xiaoni Gao; Dipti Mehta; Hans G. Lilja; Robert J. Klein
Source:
Gene 2021 v.768 pp. 145265
ISSN:
0378-1119
Subject:
CRISPR-Cas systems; RNA; alleles; carcinogenesis; cell lines; clones; correlation; gene editing; gene expression; genetic markers; genome-wide association study; heterozygosity; humans; lead; linkage disequilibrium; loci; promoter regions; prostatic neoplasms; risk; scientists; secretion; single nucleotide polymorphism
Abstract:
... GWAS have identified numerous SNPs associated with prostate cancer risk. One such SNP is rs10993994. It is located in the β-microseminoprotein (MSMB) promoter region, mediates MSMB prostate secretion levels, and is linked to mRNA expression changes in both MSMB and the adjacent gene NCOA4. In addition, our previous work showed a second SNP, rs7098889, is in positive linkage disequilibrium with rs1 ...
DOI:
10.1016/j.gene.2020.145265

48. Zinc deficiency leads to reduced interleukin-2 production by active gene silencing due to enhanced CREMα expression in T cells

Author:
Veronika Kloubert; Inga Wessels; Jana Wolf; Karoline Blaabjerg; Veerle Janssens; Jan Hapala; Wolfgang Wagner; Lothar Rink
Source:
Clinical nutrition 2021 v.40 no.5 pp. 3263-3278
ISSN:
0261-5614
Subject:
T-lymphocytes; cell lines; chronic diseases; clinical nutrition; dietary mineral supplements; elderly; epigenetics; flow cytometry; gene silencing; histone deacetylase; humans; immune response; interleukin-2; linkage (genetics); models; phosphoprotein phosphatase; secretion; swine; transcription factors; zinc
Abstract:
... The micronutrient zinc is essential for proper immune function. Consequently, zinc deficiency leads to impaired immune function, as seen in decreased secretion of interleukin (IL)-2 by T cells. Although this association has been known since the late 1980s, the underlying molecular mechanisms are still unknown. Zinc deficiency and reduced IL-2 levels are especially found in the elderly, which in tu ...
DOI:
10.1016/j.clnu.2020.10.052

49. miR-23b Attenuates LPS-Induced Inflammatory Responses in Acute Lung Injury via Inhibition of HDAC2

Author:
Zhi-Feng Luo; Xiang-Hui Jiang; Huan Liu; Li-Yuan He; Xiong Luo; Fu-Chun Chen; Yu-Lin Tan
Source:
Biochemical genetics 2021 v.59 no.2 pp. 604-616
ISSN:
0006-2928
Subject:
Western blotting; cell lines; histone deacetylase; interleukin-6; lipopolysaccharides; luciferase; lungs; macrophages; mice; microRNA; quantitative polymerase chain reaction; secretion; therapeutics; transfection
Abstract:
... Inflammatory responses play significant role in infectious etiology-induced acute lung injury (ALI). Histone deacetylase 2 is found to be essential and stimulated in lipopolysaccharide (LPS)-induced ALI by regulating proinflammatory cytokines. miR-23b has been demonstrated to be downregulated in LPS-induced inflammatory injury. In this study, we aimed to explore the interaction between miR-23b and ...
DOI:
10.1007/s10528-020-10018-7

50. A porcine alveolar macrophage cell line stably expressing CD163 demonstrates virus replication and cytokine secretion characteristics similar to primary alveolar macrophages following PRRSV infection

Author:
Yu-lin Xu; Shao-peng Wu; Yun-gang Li; Feng-xia Sun; Qiu-ju Wang; Qing Zhao; Jiang Yu; Fu-lin Tian; Jia-qiang Wu; Rui-liang Zhu; Jun Peng
Source:
Veterinary microbiology 2020 v.244 pp. 108690
ISSN:
0378-1135
Subject:
Toll-like receptors; cell lines; cytokines; genes; immunocytes; macrophages; porcine reproductive and respiratory syndrome; progeny; secretion; swine; tissue culture; virus replication; viruses
Abstract:
... The in vitro investigation of cytokine secretion induced by porcine reproductive and respiratory syndrome virus (PRRSV) requires porcine alveolar macrophages (PAMs) and their interaction with immunocytes. However, immortalized monoclonal PAMs (mPAMs) are non-permissive for PRRSV infection. The porcine CD163 receptor isolated from primary PAMs (pPAMs) confers susceptibility to PRRSV infection; thus ...
DOI:
10.1016/j.vetmic.2020.108690

51. A β-1,3/1,6-glucan from Durvillaea Antarctica inhibits tumor progression in vivo as an immune stimulator

Author:
Fan Su; Qiaoling Song; Chuanliang Zhang; Xiaohan Xu; Mengyuan Li; Dan Yao; Lijuan Wu; Xianjun Qu; Huashi Guan; Guangli Yu; Jinbo Yang; Chenyang Zhao
Source:
Carbohydrate polymers 2019 v.222 pp. 114993
ISSN:
0144-8617
Subject:
Durvillaea; algae; antibodies; antineoplastic activity; beta-glucans; cell lines; chemokines; inflammation; macrophages; models; neoplasm progression; neoplasms; phagocytosis; secretion; Antarctica
Abstract:
... β-glucans trigger the proinflammatory responses of innate immune cells to enhance the host defense. A variety of β-glucans were identified as strong immune stimulator and exerted antitumor activities. Our previous work indicates that a β-1,3/1,6-glucan (BG136) derived from marina alga Durvillaea antarctica promotes the proinflammatory responses in macrophage cell line RAW264.7. In the present stud ...
DOI:
10.1016/j.carbpol.2019.114993

52. Action modes of the immune modulating activities of crude mushroom polysaccharide from Phallus atrovolvatus

Author:
Varaporn Chaiyama; Suttipun Keawsompong; Jean Guy LeBlanc; Alejandra de Moreno de LeBlanc; Jean-Marc Chatel; Wasaporn Chanput
Source:
Bioactive carbohydrates and dietary fibre 2020 v.23 pp. 100216
ISSN:
2212-6198
Subject:
animal models; beta-glucans; body weight; boiling; cell lines; cold; colitis; colon; cytotoxicity; dietary fiber; ethanol; fruiting bodies; histology; immunomodulators; interleukin-10; interleukin-17; interleukin-6; luciferase; mechanism of action; mice; mushrooms; myeloperoxidase; secretion; spleen; sulfonic acids; transcription factor NF-kappa B; tumor necrosis factor-alpha; Thailand
Abstract:
... Phallus atrovolvatus is a wild edible mushroom which is recently being cultivated in Thailand. It contains a remarkable amount of β-(1,3–1,6)-glucan, a compound known for its immune modulating properties. Crude mushroom polysaccharide (CMP) was obtained from the fruiting body of P. atrovolvatus using boiling water extraction, followed by overnight ethanol cold precipitation. CMP is composed of app ...
DOI:
10.1016/j.bcdf.2020.100216

53. Anti-inflammatory Effect of AMPK Activators from Natural Products in RAW 264.7 Cell Model (P06-095-19)

Author:
Hong Yang; Lingling Zhao; Douglas Stevenson; Mark Bartlett; Bin Lou
Source:
Current developments in nutrition 2019 v.3 no.Supplement_1 pp. -
ISSN:
2475-2991
Subject:
AMP-activated protein kinase; Brassica oleracea; Rubus; Western blotting; anti-inflammatory activity; antibodies; broccoli; caspase-1; cell culture; cell lines; dose response; energy; enzyme-linked immunosorbent assay; genes; homeostasis; inflammation; interleukin-1beta; interleukin-6; macrophages; mice; peroxidase; protective effect; proteinase inhibitors; proteins; secretion; serine; tea; threonine
Abstract:
... AMP-activated protein kinase (AMPK) is a serine/threonine protein kinase critically involved in the regulation of cellular energy homeostasis. Many studies have suggested that AMPK activation exert significant anti-inflammatory effects. In this study, we assessed the anti-inflammatory effect of AMPK activator from natural products, Broccoli seed (BS, Brassica oleracea var. italic) extract and Swee ...
DOI:
10.1093/cdn/nzz031.P06-095-19

54. Atheroprotective effect of novel peptides from Porphyridium purpureum in RAW 264.7 macrophage cell line and its molecular docking study

Author:
Mysore Doddaiah Kavitha; Karekal Girinur Mallikarjun Gouda; Shimoga Janakirama Aditya Rao; Talkad Shivashankara Shilpa; Nandini Prasad Shetty; Ravi Sarada
Source:
Biotechnology letters 2019 v.41 no.1 pp. 91-106
ISSN:
0141-5492
Subject:
Porphyridium; cell lines; cholesterol; computer simulation; dipeptides; foam cells; interleukin-6; phycoerythrin; protein content; receptors; secretion; toxicity testing; triacylglycerols; tumor necrosis factor-alpha
Abstract:
... OBJECTIVE: To explore the atherogenic foam cell prevention efficiency of two dipeptides purified from Porphyridium purpureum on RAW 264.7 cell line and to study its molecular interaction through molecular docking. RESULT: P. purpureum consists of 29.9% protein and 2.98% phycoerythrin on a dry weight basis. The two dipeptides namely of Histidine–Glutamic acid (HE) and Glycine–Proline (GP) isolated ...
DOI:
10.1007/s10529-018-2621-5

55. Bavachin attenuates LPS-induced inflammatory response and inhibits the activation of NLRP3 inflammasome in macrophages

Author:
Yung-Li Hung; Shu-Chi Wang; Katsuhiko Suzuki; Shih-Hua Fang; Chi-Shuo Chen; Wei-Chung Cheng; Chia-Cheng Su; Hsin-Chih Yeh; Hung-Pin Tu; Po-Len Liu; Ming-Yii Huang; Chia-Yang Li
Source:
Phytomedicine 2019 v.59 pp. 152785
ISSN:
0944-7113
Subject:
Cullen corylifolium; Western blotting; adenosine triphosphate; anti-inflammatory activity; autoimmune diseases; caspase-1; cell culture; cell lines; fluorescent antibody technique; inducible nitric oxide synthase; inflammasomes; inflammation; interleukin-12; interleukin-1beta; interleukin-6; macrophages; mice; mitogen-activated protein kinase; nitric oxide; secretion; traditional medicine; transcription factor NF-kappa B; Asia
Abstract:
... Bavachin is a natural product isolated from Psoralea corylifolia L. that has been applied as a traditional medicine in Asian countries. However, the anti-inflammatory effects of bavachin on LPS-induced inflammation and NLRP3 inflammasome activation by macrophages remain unclear.We investigated the anti-inflammatory effects of bavachin on LPS-activated murine macrophage cell line J774A.1 cells and ...
DOI:
10.1016/j.phymed.2018.12.008

56. Brucella abortus Proliferates in Decidualized and Non-Decidualized Human Endometrial Cells Inducing a Proinflammatory Response

Author:
Lucía Zavattieri; Mariana C. Ferrero; Iván M. Alonso Paiva; Agustina D. Sotelo; Andrea M. Canellada; Pablo C. Baldi
Source:
Pathogens 2020 v.9 no.5 pp. -
ISSN:
2076-0817
Subject:
Brucella melitensis biovar Abortus; bacteria; brucellosis; cell lines; colchicine; cytochalasin D; cytotoxicity; dose response; endometrium; humans; infection; inflammation; interleukin-6; interleukin-8; macrophages; operon; pathogens; secretion; stromal cells
Abstract:
... Brucella spp. have been associated with abortion in humans and animals. Although the mechanisms involved are not well established, it is known that placental Brucella infection is accompanied by inflammatory phenomena. The ability of Brucella abortus to infect and survive in human endometrial stromal cells (T-HESC cell line) and the cytokine response elicited were evaluated. B. abortus was able to ...
DOI:
10.3390/pathogens9050369

57. Cellular Function of Annexin A1 Protein Mimetic Peptide Ac2-26 in Human Skin Keratinocytes HaCaT and Fibroblast Detroit 551 Cells

Author:
Seong Min Kim; Sang Eun Ha; Preethi Vetrivel; Hun Hwan Kim; Pritam Bhagwan Bhosale; Jung Eun Park; Jeong Doo Heo; Young Sil Kim; Gon Sup Kim
Source:
Nutrients 2020 v.12 no.11 pp. -
ISSN:
2072-6643
Subject:
anti-inflammatory activity; assays; cell lines; collagen; collagenase; cytotoxicity; degradation; fibroblasts; humans; inflammation; interleukin-6; interleukin-8; keratinocytes; secretion; skin (animal); skin diseases; synthesis; therapeutics; toxicity testing
Abstract:
... Inflammation of the skin is the most common dermatological problem in human. The anti-inflammatory mediated responses of the skin cells provide a mechanism for combating these conditions. Annexin A1 (AnxA1) is one of the proteins that has been shown to have a potent anti-inflammatory effect. However, the effects and mechanisms of AnxA1 in skin keratinocyte and fibroblast have not been reported yet ...
DOI:
10.3390/nu12113261

58. Chitosan-hybrid poss nanocomposites for bone regeneration: The effect of poss nanocage on surface, morphology, structure and in vitro bioactivity

Author:
Sedef Tamburaci; Funda Tihminlioglu
Source:
International journal of biological macromolecules 2020 v.142 pp. 643-657
ISSN:
0141-8130
Subject:
adhesion; alkaline phosphatase; bioactive properties; biocompatibility; biomineralization; bone formation; bones; cell lines; cell proliferation; chitosan; enzyme activity; freeze drying; nanocomposites; nanoparticles; osteoblasts; osteocalcin; polymers; porosity; secretion; silica; surface roughness; tissue repair
Abstract:
... POSS, regarded as the smallest silica particle, is widely used as nanofiller in polymer systems. POSS-based nanocomposites are deduced as novel materials having potency for biomedical applications owing to the enhanced biocompatibility and physicochemical characteristics. The aim of this work was to integrate the beneficial features of chitosan (CS) and OctaTMA-POSS nanoparticle to design nanocomp ...
DOI:
10.1016/j.ijbiomac.2019.10.006

59. Circulating exosomal and Argonaute-bound microRNAs in preeclampsia

Author:
Orsolya Biró; Ábel Fóthi; Bálint Alasztics; Bálint Nagy; Tamás I. Orbán; János Rigó
Source:
Gene 2019 v.692 pp. 138-144
ISSN:
0378-1119
Subject:
altitude; blood flow; blood sampling; byproducts; cell communication; cell culture; cell death; cell lines; etiology; exosomes; hypoxia; microRNA; pre-eclampsia; pregnancy; proteins; quantitative polymerase chain reaction; secretion; spectroscopy; trophoblast
Abstract:
... microRNAs (miRNAs) play important role in the regulation of placental development, and abnormal miRNA expression is associated with preeclampsia (PE). miRNAs are released from trophoblast cells to maternal blood flow, where they are highly stable, being encapsulated inside extracellular vesicles, like exosomes or bound to Argonaute proteins. In PE, placental dysfunction leads to aberrant extracell ...
DOI:
10.1016/j.gene.2019.01.012

60. DGLA from the Microalga Lobosphaera Incsa P127 Modulates Inflammatory Response, Inhibits iNOS Expression and Alleviates NO Secretion in RAW264.7 Murine Macrophages

Author:
Ekaterina Novichkova; Katya Chumin; Noy Eretz-Kdosha; Sammy Boussiba; Jacob Gopas; Guy Cohen; Inna Khozin-Goldberg
Source:
Nutrients 2020 v.12 no.9 pp. -
ISSN:
2072-6643
Subject:
Lobosphaera incisa; arachidonic acid; cell lines; dose response; immune response; inflammation; interleukin-6; lipopolysaccharides; macrophages; mice; microalgae; mutants; nitric oxide; prostaglandins; reactive oxygen species; secretion; tumor necrosis factor-alpha
Abstract:
... Microalgae have been considered as a renewable source of nutritional, cosmetic and pharmaceutical compounds. The ability to produce health-beneficial long-chain polyunsaturated fatty acids (LC-PUFA) is of high interest. LC-PUFA and their metabolic lipid mediators, modulate key inflammatory pathways in numerous models. In particular, the metabolism of arachidonic acid under inflammatory challenge i ...
DOI:
10.3390/nu12092892

61. Deacetyl Ganoderic Acid F Inhibits LPS-Induced Neural Inflammation via NF-κB Pathway Both In Vitro and In Vivo

Author:
Feiya Sheng; Lele Zhang; Songsong Wang; Lele Yang; Peng Li
Source:
Nutrients 2019 v.12 no.1 pp. -
ISSN:
2072-6643
Subject:
Danio rerio; Ganoderma lucidum; anti-inflammatory activity; astrocytes; blood serum; brain; cell lines; ganoderic acid; inflammation; interleukin-6; lipopolysaccharides; medicinal fungi; mice; neurodegenerative diseases; neurons; nutrients; phosphorylation; secretion; sleep disorders; therapeutics; traditional medicine
Abstract:
... Microglia mediated neuronal inflammation has been widely reported to be responsible for neurodegenerative disease. Deacetyl ganoderic acid F (DeGA F) is a triterpenoid isolated from Ganoderma lucidum, which is a famous edible and medicinal mushroom used for treatment of dizziness and insomnia in traditional medicine for a long time. In this study the inhibitory effects and mechanisms of DeGA F aga ...
DOI:
10.3390/nu12010085

62. Downregulation of A2AR by siRNA loaded PEG-chitosan-lactate nanoparticles restores the T cell mediated anti-tumor responses through blockage of PKA/CREB signaling pathway

Author:
Ali Masjedi; Hadi Hassannia; Fatemeh Atyabi; Ali Rastegari; Mohammad Hojjat-Farsangi; Afshin Namdar; Hassan Soleimanpour; Gholamreza Azizi; Afshin Nikkhoo; Ghasem Ghalamfarsa; Abbas Mirshafiey; Farhad Jadidi-Niaragh
Source:
International journal of biological macromolecules 2019 v.133 pp. 436-445
ISSN:
0141-8130
Subject:
T-lymphocytes; adenosine; apoptosis; binding proteins; breasts; cAMP-dependent protein kinase; cell lines; cytokines; gene expression regulation; immunotherapy; mice; nanoparticles; neoplasms; physicochemical properties; polyethylene glycol; receptors; secretion; signal transduction; small interfering RNA; toxicity; transcription factor NF-kappa B; transfection; zeta potential
Abstract:
... Adenosine and its receptors are novel promising targets for cancer immunotherapy. In here, we aimed to evaluate the efficacy of Polyethylene glycol (PEG)-chitosan-lactate (PCL) nanoparticles (NPs) loaded with A2AR-specific siRNA for interfering with differentiation and function of T cells derived from the 4T1 breast tumor-bearing Balb/C mice, ex vivo. The size of synthesized NPs was about 100 nm i ...
DOI:
10.1016/j.ijbiomac.2019.03.223

63. Encapsulated human mesenchymal stem cells (eMSCs) as a novel anti-cancer agent targeting breast cancer stem cells: Development of 3D primed therapeutic MSCs

Author:
Saurabh Mandal; Frank Arfuso; Gautam Sethi; Arun Dharmarajan; Sudha Warrier
Source:
international journal of biochemistry & cell biology 2019 v.110 pp. 59-69
ISSN:
1357-2725
Subject:
Western blotting; angiogenesis; antagonists; antineoplastic activity; antineoplastic agents; biocompatible materials; breast neoplasms; breasts; cadherins; caspases; cell lines; cell viability; encapsulation; epithelium; gene expression; gene expression regulation; genes; humans; immunomodulation; interferon-gamma; interleukin-10; mesenchymal stromal cells; microbeads; mortality; neoplasm cells; nitric oxide; reactive oxygen species; secretion; signal transduction; sodium alginate; stem cells; therapeutics; transforming growth factor beta; transporters; tumor necrosis factor-alpha; vascular endothelial growth factors; women
Abstract:
... Breast cancer is a leading cause of mortality in women across the globe. The major reason for its recurrence and high mortality is due to the presence of a drug refractory and self-renewing population of cells, the cancer stem cells (CSCs). Mesenchymal stem cells (MSCs) have recently emerged as a promising cell-based therapeutic agent for the treatment of different cancer types. However, the anti- ...
DOI:
10.1016/j.biocel.2019.02.001

64. Evaluation of a Brown Seaweed Extract from Dictyosiphon foeniculaceus as a Potential Therapeutic Agent for the Treatment of Glioblastoma and Uveal Melanoma

Author:
Philipp Dörschmann; Christina Schmitt; Kaya Saskia Bittkau; Sandesh Neupane; Michael Synowitz; Johann Roider; Susanne Alban; Janka Held-Feindt; Alexa Klettner
Source:
Marine drugs 2020 v.18 no.12 pp. -
ISSN:
1660-3397
Subject:
Dictyosiphon; antineoplastic activity; astrocytes; cell lines; cell viability; drugs; enzyme-linked immunosorbent assay; epithelium; eyes; glioblastoma; humans; ingredients; melanoma; neoplasm cells; plant extracts; quantitative polymerase chain reaction; research; secretion; testing; therapeutics; toxicity; vascular endothelial growth factor A; vascular endothelial growth factor receptor-1; vascular endothelial growth factor receptor-2
Abstract:
... Ingredients of brown seaweed like fucoidans are often described for their beneficial biological effects, that might be interesting for a medical application. In this study, we tested an extract from Dictyosiphon foeniculaceus (DF) to evaluate the effects in glioblastoma and uveal melanoma, looking for a possible anti-cancer treatment. We investigated toxicity, VEGF (vascular endothelial growth fac ...
DOI:
10.3390/md18120625

65. Evaluation of the therapeutic effect against benign prostatic hyperplasia and the active constituents from Epilobium angustifolium L.

Author:
Liqing Deng; Wei Zong; Xueying Tao; Shuang Liu; Zhiying Feng; Yuping Lin; Zhihua Liao; Min Chen
Source:
Journal of ethnopharmacology 2019 v.232 pp. 1-10
ISSN:
0378-8741
Subject:
in vivo studies; secretion; chromatography; propionic acid; cell lines; epithelium; oral administration; toxicity testing; oxidative stress; cell viability; neoplasms; urethra; Epilobium angustifolium; enzyme-linked immunosorbent assay; ethyl acetate; butanol; screening; spectroscopy; traditional medicine; transcription factor NF-kappa B; testosterone; rats; histopathology; hyperplasia; prostate-specific antigen; inflammation; chemical constituents of plants; active ingredients
Abstract:
... Plants of Epilobium angustifolium are popular in China to treatment of traumatic injury, subduing inflammation and menstrual disorders. In European, the preparations or extracts containing E. angustifolium are popular to treat prostate diseases. Recent research suggested that E. angustifolium showed therapeutic effects in early stage of BPH, inflammation of urethra and prostate, as well as micturi ...
DOI:
10.1016/j.jep.2018.11.045

66. Functional analysis of Bucella reveals transcriptional regulation of MarR

Author:
Xingmei Deng; Min Li; Liangbo Liu; Jing Zhang; Yu Zhang; Jia Guo; Tianyi Zhao; Shuzhu Cao; Zhiqiang Li; Hui Zhang
Source:
Microbial pathogenesis 2020 v.144 pp. 104201
ISSN:
0882-4010
Subject:
Brucella melitensis biovar Abortus; brucellosis; cell lines; chemoattractants; chromatin immunoprecipitation; gene expression; genome; high-throughput nucleotide sequencing; inflammation; interferon-gamma; interleukin-12; interleukin-1beta; interleukin-6; macrophages; mice; mutants; pathogens; secretion; transcription (genetics); transcription factors; tumor necrosis factor-alpha; virulence; zoonoses
Abstract:
... Brucellosis is a zoonotic infectious disease caused by Brucella infection. MarR-family transcription factors are closely related to diverse physiological functions necessary for many pathogens adaptation to environmental changes. However, whether the MarR-family transcription factors are involved in virulence, mediated inflammatory responses and regulated virulence gene expression in the intracell ...
DOI:
10.1016/j.micpath.2020.104201

67. GDNF synthesis, signaling, and retrograde transport in motor neurons

Author:
Alberto F. Cintrón-Colón; Gabriel Almeida-Alves; Alicia M. Boynton; John M. Spitsbergen
Source:
Cell and tissue research 2020 v.382 no.1 pp. 47-56
ISSN:
0302-766X
Subject:
amino acids; amyotrophic lateral sclerosis; brain; cell lines; dopamine; exercise; ligands; motor neurons; neuroglia; neuroprotective effect; phosphorylation; rats; receptor protein-tyrosine kinase; receptors; research; secretion; signal transduction; synthesis
Abstract:
... Glial cell line–derived neurotrophic factor (GDNF) is a 134 amino acid protein belonging in the GDNF family ligands (GFLs). GDNF was originally isolated from rat glial cell lines and identified as a neurotrophic factor with the ability to promote dopamine uptake within midbrain dopaminergic neurons. Since its discovery, the potential neuroprotective effects of GDNF have been researched extensively ...
DOI:
10.1007/s00441-020-03287-6

68. Identification of goldfish (Carassius auratus L.) leukocyte immune-type receptors shows alternative splicing as a potential mechanism for receptor diversification

Author:
Jiahui Wang; Miodrag Belosevic; James L. Stafford
Source:
Molecular immunology 2020 v.125 pp. 83-94
ISSN:
0161-5890
Subject:
Carassius auratus; Ictalurus punctatus; cell lines; cytokines; goldfish; immunologic receptors; immunomodulation; kidneys; macrophages; mammals; multigene family; neutrophils; phagocytosis; phylogeny; secretion
Abstract:
... Leukocyte immune-type receptors (LITRs) are a multigene family of teleost immunoregulatory proteins that share structural, phylogenetic, and likely functional relationships with several innate immune receptor proteins in other vertebrates, including mammals. Originally discovered in channel catfish (Ictalurus punctatus), representative IpLITR-types have been shown to regulate diverse innate immune ...
DOI:
10.1016/j.molimm.2020.06.024

69. Immunomodulatory and morphophysiological effects of Rhipicephalus sanguineus s. l. (Acari: Ixodidae) salivary gland extracts

Author:
Marina Rodrigues de Abreu; Melissa Carolina Pereira; Patrícia Ucelli Simioni; Elen Fernanda Nodari; Lisiery Negrini Paiatto; Maria Izabel Camargo-Mathias
Source:
Veterinary immunology and immunopathology 2019 v.207 pp. 36-45
ISSN:
0165-2427
Subject:
Rhipicephalus sanguineus; cell lines; cytotoxicity; dogs; females; hosts; humans; immunomodulators; immunosuppression; interleukin-10; interleukin-2; interleukin-6; macrophages; microscopy; monocytes; nitric oxide; parasitism; pathogens; rabbits; saliva; salivary glands; secretion; tick control; ticks; tumor necrosis factor-alpha
Abstract:
... Rhipicephalus sanguineus s. l. is popularly known as the “brown dog tick” since dogs are its preferential hosts, but the species has been reported to parasitize other mammals, including humans, with significant medical-veterinary importance since it transmits several important pathogenic agents during the feeding period. The tick saliva is a complex mixture that has several functions, including th ...
DOI:
10.1016/j.vetimm.2018.11.017

70. In-vitro (2D and 3D cultures) and in-vivo cytotoxic properties of Zataria multiflora essential oil (ZEO) emulsion in breast and cervical cancer cells along with the investigation of immunomodulatory potential

Author:
Mohadeseh Azadi; Tahereh Jamali; Zahra Kianmehr; Gholamreza Kavoosi; Susan Kaboudanian Ardestani
Source:
Journal of ethnopharmacology 2020 v.257 pp. 112865
ISSN:
0378-8741
Subject:
CD4-positive T-lymphocytes; Zataria multiflora; alanine transaminase; antineoplastic activity; apoptosis; aspartate transaminase; breasts; caspase-3; cell culture; cell lines; cell proliferation; cytotoxicity; death; emulsions; enzyme activity; essential oils; fluorescence; immunomodulators; inflammation; interferon-gamma; interleukin-2; interleukin-4; mammary neoplasms (animal); mice; neoplasm cells; pain; secretion; staining; toxicity testing; traditional medicine; tumor necrosis factor-alpha; uterine cervical neoplasms
Abstract:
... Zataria multiflora is an iranian valuable traditional plants, called Avishan Shirazi in Persian language used to reduce inflammation, spasm, pain, and cancer symptoms. Zataria essential oil (ZEO) is one of the essential oils possessing broad biological activities.The aim was to investigate the anticancer effects of ZEO both in-vitro and in-vivo using mouse mammary carcinoma 4T1 cell line and mouse ...
DOI:
10.1016/j.jep.2020.112865

71. Inflammatory mechanism of Chlamydia trachomatis-infected HeLa229 cells regulated by Atg5

Author:
Fuyan Wang; Lei Zhang; Xiaofang Lu; Quan Zhu; Tingting Shi; Rong Lu; Ping Yu; Hongbo Zhang; Yong Wang
Source:
Biochemical and biophysical research communications 2019 v.520 no.1 pp. 205-210
ISSN:
0006-291X
Subject:
Chlamydia trachomatis; autophagy; cell lines; interleukin-10; interleukin-6; models; research; secretion
Abstract:
... The relation between autophagy and Chlamydia trachomatis infection remain inconclusive. In order to further understand the role of autophagy in C. trachomatis-infected cells. Atg5 silenced HeLa229 cell line was used to establish an autophagy inhibition C. trachomatis infection model. The results suggested that Atg5 served a key regulatory role in the autophagy of C. trachomatis-infected cells. Sil ...
DOI:
10.1016/j.bbrc.2019.09.132

72. Investigating the cytotoxic effects of the venom proteome of two species of the Viperidae family (Cerastes cerastes and Cryptelytrops purpureomaculatus) from various habitats

Author:
Cenk Serhan Ozverel; Maik Damm; Benjamin-Florian Hempel; Bayram Göçmen; Robert Sroka; Roderich D. Süssmuth; Ayse Nalbantsoy
Source:
Comparative biochemistry and physiology 2019 v.220 pp. 20-30
ISSN:
1532-0456
Subject:
Viperidae; antineoplastic agents; cell lines; coastal forests; cytotoxicity; glioblastoma; habitats; humans; inhibitory concentration 50; mass spectrometry; neoplasm cells; peptides; proteome; secretion; snake venoms; snakes; therapeutics; Northern Africa; South East Asia
Abstract:
... Animal secretions are of great interest in terms of drug development due to their complex protein and peptide composition. Especially, in the field of therapeutic medications such as anti-cancer drugs snake venoms receive attention. In this study, we address two Viperidae species from various habitats with a particular focus on the cytotoxic potential along with the decomplexation of the venom pro ...
DOI:
10.1016/j.cbpc.2019.02.013

73. Investigation of the chemical components of ambient fine particulate matter (PM2.5) associated with in vitro cellular responses to oxidative stress and inflammation

Author:
Fanfan Xu; Xiaodi Shi; Xinghua Qiu; Xing Jiang; Yanhua Fang; Junxia Wang; Di Hu; Tong Zhu
Source:
Environment international 2020 v.136 pp. 105475
ISSN:
0160-4120
Subject:
air pollution; bioactive properties; cell lines; coal; combustion; data collection; human health; inflammation; interleukin-1beta; least squares; macrophages; methylene chloride; models; oxidative stress; particulate emissions; particulates; polycyclic aromatic hydrocarbons; reactive oxygen species; risk; secretion; transition elements; tumor necrosis factor-alpha
Abstract:
... Fine particulate matter (PM₂.₅) poses a significant risk to human health worldwide, by promoting oxidative stress and inflammation; however, the components responsible for these effects have not been fully evaluated. In this study, we investigated the cellular response of a macrophage cell line exposed to PM₂.₅ extracts in vitro. We obtained a dataset of chemical components of PM₂.₅ and determined ...
DOI:
10.1016/j.envint.2020.105475

74. Isolation and comparison of α- and β-D-glucans from shiitake mushrooms (Lentinula edodes) with different biological activities

Author:
Diego Morales; Renata Rutckeviski; Marisol Villalva; Hellen Abreu; Cristina Soler-Rivas; Susana Santoyo; Marcello Iacomini; Fhernanda Ribeiro Smiderle
Source:
Carbohydrate polymers 2020 v.229 pp. 115521
ISSN:
0144-8617
Subject:
2,2-diphenyl-1-picrylhydrazyl; Fourier transform infrared spectroscopy; Lentinula edodes; antioxidant activity; beta-glucans; breast neoplasms; breasts; cell lines; chemical structure; colorimetry; cytotoxicity; fluorometry; gas chromatography-mass spectrometry; in vivo studies; interleukin-1beta; interleukin-6; mushrooms; neoplasm cells; nuclear magnetic resonance spectroscopy; secretion
Abstract:
... A polysaccharide-enriched extract obtained from Lentinula edodes was submitted to several purification steps to separate three different D-glucans with β-(1→6), β-(1→3),(1→6) and α-(1→3) linkages, being characterized through GC–MS, FT-IR, NMR, SEC and colorimetric/fluorimetric determinations. Moreover, in vitro hypocholesterolemic, antitumoral, anti-inflammatory and antioxidant activities were als ...
DOI:
10.1016/j.carbpol.2019.115521

75. Lignans from Schisandra chinensis ameliorate alcohol and CCl4-induced long-term liver injury and reduce hepatocellular degeneration via blocking ETBR

Author:
Jin-Biao Xu; Guang-Chun Gao; Ming-Jing Yuan; Xuan Huang; Hong-Yu Zhou; Yang Zhang; Ya-Xin Zheng; Zhe Wu; Jun-Miao Feng; Ji-Ming Wu
Source:
Journal of ethnopharmacology 2020 v.258 pp. 112813
ISSN:
0378-8741
Subject:
Oriental traditional medicine; Schisandra chinensis; Western blotting; antioxidants; apoptosis; blood serum; cell lines; computer simulation; disease severity; endoplasmic reticulum; endothelin receptors; enzymes; ethanol; fibrosis; genes; glutathione; hepatitis; hepatocytes; hepatoprotective effect; hepatotoxicity; histology; lignans; liver; liver cirrhosis; liver neoplasms; mice; microarray technology; middle-aged adults; models; patients; prediction; secretion; transforming growth factor beta 1; vines
Abstract:
... Chemical hepatotoxicity, especially alcoholic liver injury (ALI), commonly occurs in young and middle-aged people who drink heavily. ALI is extremely harmful and can induce severe disease states, such as hepatitis, liver fibrosis, cirrhosis, or liver cancer, which are similar to CCl₄-induced liver disease states in animals. In recent studies, the pathological changes of hepatocytes and the hepatic ...
DOI:
10.1016/j.jep.2020.112813

76. Liraglutide ameliorates palmitate-induced oxidative injury in islet microvascular endothelial cells through GLP-1 receptor/PKA and GTPCH1/eNOS signaling pathways

Author:
Yunyi Le; Rui Wei; Kun Yang; Shan Lang; Liangbiao Gu; Junling Liu; Tianpei Hong; Jin Yang
Source:
Peptides 2020 v.124 pp. 170212
ISSN:
0196-9781
Subject:
antagonists; apoptosis; cAMP-dependent protein kinase; cell lines; dose response; endothelial cells; endothelial nitric oxide synthase; endothelins; enzyme inhibitors; gene expression; glucagon-like peptide 1; guanosine; guanosine triphosphate; islets of Langerhans; lipotoxicity; messenger RNA; mice; models; noninsulin-dependent diabetes mellitus; oxidative stress; oxidative toxicity; palmitic acid; pro-apoptotic proteins; protective effect; protein content; reactive oxygen species; secretion; signal transduction
Abstract:
... In type 2 diabetes, lipotoxicity damages islet microvascular endothelial cells (IMECs), leading to pancreatic islet β cell dysfunction directly or indirectly. Glucagon-like peptide-1 (GLP-1) and its analogs have beneficial roles in endothelial cells. However, the protective effects of GLP-1 agents on IMECs and their potential mechanism remained obscure. In this study, exposure of MS-1 (a cell line ...
DOI:
10.1016/j.peptides.2019.170212

77. Macrophage-derived extracellular vesicles regulate concanavalin A-induced hepatitis by suppressing macrophage cytokine production

Author:
Reo Kawata; Shingo Oda; Yoshihiro Koya; Hiroaki Kajiyama; Tsuyoshi Yokoi
Source:
Toxicology 2020 v.443 pp. 152544
ISSN:
0300-483X
Subject:
RNA interference; animal models; blood serum; cell lines; concanavalin A; cytokines; genes; hepatitis; hepatoma; immunomodulation; in vitro studies; inflammation; intravenous injection; liver; liver failure; macrophages; messenger RNA; mice; microRNA; mitogen-activated protein kinase; neoplasm cells; phosphatidylinositol 3-kinase; secretion; sequence analysis
Abstract:
... Acute liver failure is a clinical syndrome of severe hepatic dysfunction. Immune cells play an important role in acute liver failure. In recent years, the immunoregulatory function of extracellular vesicles (EVs) has been reported; therefore, it is inferred that EVs play a role in immune-mediated hepatitis. In this study, we investigated the immunoregulatory function of EVs in concanavalin A (Con ...
DOI:
10.1016/j.tox.2020.152544

78. Releasing Intracellular NS1 from Mosquito Cells for the Detection of Dengue Virus-Infected Mosquitoes

Author:
Lie Cheng; Wei-Liang Liu; Hsing-Han Li; Matthew P. Su; Shih-Cheng Wu; Hsin-Wei Chen; Chao-Ying Pan; Jih-Jin Tsai; Chun-Hong Chen
Source:
Viruses 2020 v.12 no.10 pp. -
ISSN:
1999-4915
Subject:
Aedes aegypti; Dengue virus; cell lines; control methods; dengue; detection limit; disease control; disease outbreaks; immunoblotting; midgut; monitoring; mosquito control; octoxynol; pathogens; quantitative polymerase chain reaction; rapid methods; risk; secretion; vector-borne diseases
Abstract:
... Dengue virus (DENV), the pathogen that causes dengue fever, is mainly transmitted by Aedes aegypti. Surveillance of infected mosquitoes is a major component of integrated mosquito control methods for reducing the risk of vector-born disease outbreaks. However, a specialized rapid test for DENV detection in mosquitoes is not currently available. Utilizing immunoblotting, we found that the secretion ...
DOI:
10.3390/v12101105

79. Secretory leakage of IgG1 aggregates from recombinant Chinese hamster ovary cells

Author:
Masayoshi Onitsuka; Yukinori Kadoya; Takeshi Omasa
Source:
Journal of bioscience and bioengineering 2019 v.127 no.6 pp. 752-757
ISSN:
1389-1723
Subject:
Chinese hamsters; animal ovaries; antibodies; bioreactors; byproducts; cell culture; cell lines; cell viability; gel chromatography; immunogenicity; immunoglobulin G; manufacturing; quality control; secretion; therapeutics
Abstract:
... Aggregation of therapeutic antibodies is one of the most important issues to be resolved in manufacturing processes because of reduced efficacy and immunogenicity. Despite aggregation studies in vitro, little is known about the aggregation mechanism in cell culture processes. In this study, we investigated the process of aggregate formation of IgG1 antibodies during the culture of Chinese hamster ...
DOI:
10.1016/j.jbiosc.2018.11.015

80. Sodium butyrate promotes lipopolysaccharide-induced innate immune responses by enhancing mitogen-activated protein kinase activation and histone acetylation in bovine mammary epithelial cells

Author:
Hongyu Dai; Guozhen Wei; Yan Wang; Nana Ma; Guangjun Chang; Xiangzhen Shen
Source:
Journal of dairy science 2020 v.103 no.12 pp. 11636-11652
ISSN:
0022-0302
Subject:
Escherichia coli; acetylation; bovine mastitis; cattle; cell lines; cell viability; chromatin; dose response; energy; enzyme inhibitors; epithelial cells; gene expression; gene expression regulation; genes; heat inactivation; histones; innate immunity; interleukin-8; lipopolysaccharides; mammary glands; mitogen-activated protein kinase; pathogens; secretion; sodium butyrate; transcription (genetics); tumor necrosis factors; viability assays
Abstract:
... The innate immune response plays a crucial role in recovery from infectious diseases by promoting the clearance of pathogens. Sodium butyrate (NaB) is an energy source for cellular processes with the potential to regulate the innate immune response. The present study aimed to evaluate the effect of NaB on the innate immune response in a bovine mammary alveolar cell line (MAC-T) initiated by lipopo ...
DOI:
10.3168/jds.2020-18198

81. Sophorasubprosrate polysaccharide suppress the inflammatory reaction of RAW264.7 cells infected with PCV2 via regulation NF-κB/MAPKs/c-Jun signal pathway and histone acetylation modification

Author:
Jian Yang; Mi-xia Cao; Wen-yue Hu; Ying-yi Wei; Ting-jun Hu
Source:
International journal of biological macromolecules 2020 v.159 pp. 957-965
ISSN:
0141-8130
Subject:
Oriental traditional medicine; Porcine circovirus-2; acetylation; cell lines; gene expression; histones; inflammation; interleukin-10; interleukin-6; macrophages; mice; phosphorylation; polysaccharides; secretion; signal transduction
Abstract:
... The purpose of this study was to investigate the regulation of Sophorasubprosrate polysaccharide (SSP) on inflammatory response and histone acetylation modification of RAW264.7 cells (mouse mononuclear macrophage cell line) infected with porcine circovirus type 2 (PCV2). We further explored the role of inflammatory response and histone acetylation modification on the basis of the original study. T ...
DOI:
10.1016/j.ijbiomac.2020.05.128

82. Specific high affinity interaction of Helicobacter pylori CagL with integrin αVβ6 promotes type IV secretion of CagA into human cells

Author:
Maren Buß; Nicole Tegtmeyer; Jennifer Schnieder; Xianchi Dong; Jing Li; Timothy A. Springer; Steffen Backert; Hartmut H. Niemann
Source:
FEBS journal 2019 v.286 no.20 pp. 3980-3997
ISSN:
1742-464X
Subject:
Helicobacter pylori; cell adhesion; cell lines; epithelial cells; fimbriae; gastric mucosa; humans; integrins; interleukin-8; leucine; mutation; secretion; type IV secretion system
Abstract:
... CagL is an essential pilus surface component of the virulence‐associated type IV secretion system (T4SS) employed by Helicobacter pylori to translocate the oncogenic effector protein CagA into human gastric epithelial cells. CagL contains an RGD motif and integrin α₅β₁ is widely accepted as its host cell receptor. Here, we show that CagL binds integrin αVβ₆ with substantially higher affinity and t ...
DOI:
10.1111/febs.14962

83. Stem Cell Membrane-Coated Au-Ag-PDA Nanoparticle-Guided Photothermal Acne Therapy

Author:
Tianqi Meng; Rihua Jiang; Shiyi Wang; Jing Li; Fuqiang Zhang; Jeung-Hoon Lee; Jinlan Jiang; Mingji Zhu
Source:
Colloids and surfaces 2020 v.192 pp. 111145
ISSN:
0927-7765
Subject:
acne; cell lines; cell membranes; cell proliferation; cytotoxicity; histology; photothermotherapy; sebum; secretion; stem cells
Abstract:
... The polydopamine coating on Au-Ag nanoparticles (Au-Ag-PDA) possess excellent photothermal conversion efficiency after absorbing near-infrared laser light. After the stem cell membrane (STCM) encapsulates Au-Ag-PDA (Au-Ag-PDA@STCM), the nanoparticles (NPs) exhibit less cytotoxicity, and further optimizing their efficiency in photothermal therapy. The photothermal activity of Au-Ag-PDA@STCM has not ...
DOI:
10.1016/j.colsurfb.2020.111145

84. Structural characterisation and immunomodulatory activity of exopolysaccharides from liquid fermentation of Monascus purpureus (Hong Qu)

Author:
Nifei Wang; Gege Jia; Changlu Wang; Mianhua Chen; Fengjiao Xie; N.V. Nepovinnykh; H. Douglas Goff; Qingbin Guo
Source:
Food hydrocolloids 2020 v.103 pp. 105636
ISSN:
0268-005X
Subject:
Fourier transform infrared spectroscopy; Monascus purpureus; arabinose; cell lines; cell proliferation; chemical structure; chromatography; edible fungi; endocytosis; exopolysaccharides; galactose; gamma-aminobutyric acid; glucose; hydrocolloids; immunomodulators; industrial applications; interleukin-10; interleukin-6; liquid state fermentation; mannose; messenger RNA; methylation; nitric oxide; nuclear magnetic resonance spectroscopy; pigments; rhamnose; secondary metabolites; secretion; tumor necrosis factor-alpha; xylose
Abstract:
... As a traditional Chinese medicinal and edible fungus, Monascus has been widely studied for its secondary metabolites such as Monascus pigments and γ-aminobutyric acid. However, the exopolysaccharides have not been extensively studied and this has limited their food and industrial applications. In this study, exopolysaccharides isolated from the liquid fermentation of Monascus purpureus (Hong Qu) w ...
DOI:
10.1016/j.foodhyd.2019.105636

85. Taraxastane-type triterpenoids from the medicinal and edible plant Cirsium setosum

Author:
Peng-Cheng LIN; Lin-Lin JI; Xiang-Jian ZHONG; Jin-Jie LI; Xin WANG; Xiao-Ya SHANG; Sheng LIN
Source:
Chinese journal of natural medicines 2019 v.17 no.1 pp. 22-26
ISSN:
1875-5364
Subject:
Cirsium arvense; Oriental traditional medicine; animal ovaries; antineoplastic activity; cell lines; chemical structure; colorectal neoplasms; cytotoxicity; food plants; humans; inhibitory concentration 50; ovarian neoplasms; secretion; spectral analysis; triterpenoids; tumor necrosis factor-alpha
Abstract:
... Guided by TNF-α secretion inhibitory activity assay, four taraxastane-type triterpenoids, including two new ones, 22-oxo-20-taraxasten-3β, 30-diol (1) and 22α-hydroxy-20-taraxasten-30β, 30-triol (2), have been obtained from an active fraction of the petroleum ether-soluble extract of the the medicinal and edible plant Cirsium setosum. Their structures were elucidated by spectroscopic data and CD d ...
DOI:
10.1016/S1875-5364(19)30005-6

86. The role of autonomously secreted PGE2 and its autocrine/paracrine effect on bone matrix mineralization at the different stages of differentiating MC3T3-E1 cells

Author:
Hiraku Suzuki; Noriyasu Ohshima; Kazuaki Tatei; Tomoyo Taniguchi; Seiichi Sato; Takashi Izumi
Source:
Biochemical and biophysical research communications 2020 pp. -
ISSN:
0006-291X
Subject:
autocrine signaling; bone marrow; cell lines; coculture; culture media; enzymes; mineralization; osteoblasts; prostaglandins; research; secretion
Abstract:
... Bone is consisted of osteoblast-linage cells, bone-forming cells in various differentiation stages. However, it is not fully understood how communicate and interact these cells immigrated from bone marrow. In this study, we showed that prostaglandin E2 (PGE₂) had a role in autonomous modification of matrix mineralization in osteoblastic cell line, MC3T3-E1, and interactions across the cells in dif ...
DOI:
10.1016/j.bbrc.2020.01.120

87. Triacylglycerols accumulation and glycolipids secretion by the oleaginous yeast Rhodotorula babjevae Y-SL7: Structural identification and biotechnological applications

Author:
Mohamed Guerfali; Ines Ayadi; Nadia Mohamed; Wajdi Ayadi; Hafedh Belghith; Maria Rosário Bronze; Maria H.L. Ribeiro; Ali Gargouri
Source:
Bioresource technology 2019 v.273 pp. 326-334
ISSN:
0960-8524
Subject:
Rhodotorula; analytical methods; biosurfactants; carbon; cell lines; cytotoxicity; emulsifying properties; fatty acids; glycolipids; hydrophobicity; microbial oils; neoplasm cells; neoplasms; nitrogen; polyols; secretion; therapeutics; triacylglycerols; yeasts
Abstract:
... The newly isolated oleaginous yeast, Rhodotorula babjevae Y-SL7, was shown to accumulate high intracellular content of microbial oil (mainly triacylglycerols) and to secret, under the same culture conditions, an atypical glycolipid. This unusual behavior was induced when the strain was subjected to nitrogen limitation and high amount of carbon. A series of analytical methods was adopted in order t ...
DOI:
10.1016/j.biortech.2018.11.036

88. Venenum bufonis: An overview of its traditional use, natural product chemistry, pharmacology, pharmacokinetics and toxicology

Author:
Wen-Long Wei; Jin-Jun Hou; Xia Wang; Yang Yu; Hao-Jv Li; Zhen-Wei Li; Zi-Jin Feng; Hua Qu; Wan-Ying Wu; De-An Guo
Source:
Journal of ethnopharmacology 2019 v.237 pp. 215-235
ISSN:
0378-8741
Subject:
Bufo; Oriental traditional medicine; adverse effects; anesthetics; animals; antimicrobial properties; antineoplastic activity; bioavailability; biochemical pathways; bufadienolides; cardiotoxicity; cell lines; clinical trials; databases; diarrhea; heat stroke; hydroxylation; indole alkaloids; metabolites; neoplasm cells; neoplasms; new drugs; pain; pharmacodynamics; pharmacokinetics; secretion; toxicology; vomiting
Abstract:
... The animal medicine of Venenum Bufonis (VB), a product of the secretions of Bufo gargarizans Cantor or B. melanostictus Schneider, has long been used as a traditional Chinese medicine (TCM) for the treatment of sunstroke and faint, acute filthy disease - abdominal pain or vomiting and diarrhea, etc.This review is aimed at providing the comprehensive and up-to-date information of VB as regards its ...
DOI:
10.1016/j.jep.2019.03.042

89. Zinc Oxide Exerts Anti-Inflammatory Properties on Human Placental Cells

Author:
Andrea Balduit; Alessandro Mangogna; Chiara Agostinis; Gabriella Zito; Federico Romano; Giuseppe Ricci; Roberta Bulla
Source:
Nutrients 2020 v.12 no.6 pp. -
ISSN:
2072-6643
Subject:
adhesion; cell lines; chemokine CCL2; dietary supplements; enzyme-linked immunosorbent assay; exocytosis; humans; inflammation; intercellular adhesion molecule-1; interleukin-6; necrosis; neoplasms; pregnancy; secretion; trophoblast; vascular cell adhesion molecules; zinc oxide
Abstract:
... Background: An aberrant and persistent inflammatory state at the fetal-maternal interface is considered as a key contributor in compromised pregnancies. Decidual endothelial cells (DECs) play a pivotal role in the control of the local decidual inflammation. The aim of the current study was to determine whether dietary supplement with zinc oxide (ZnO), due to its very low adverse effects, may be us ...
DOI:
10.3390/nu12061822

90. “Spexin improves adipose tissue inflammation and macrophage recruitment in obese mice”

Author:
Sabrina Eliana Gambaro; María Guillermina Zubiría; Alejandra Paula Giordano; Andrea Estefanía Portales; Ana Alzamendi; Martín Rumbo; Andrés Giovambattista
Source:
Biochimica et biophysica acta 2020 v.1865 no.7 pp. 158700
ISSN:
1388-1981
Subject:
adipocytes; adipose tissue; adults; animal disease models; cell lines; coculture; energy balance; epididymis; fructose; gastrointestinal system; glucose; homeostasis; hypertrophy; in vitro studies; inflammation; insulin; interleukin-10; interleukin-1beta; interleukin-6; laboratory animals; leptin; lipid metabolism; liver; macrophage activation; macrophages; messenger RNA; mice; monocytes; obesity; secretion; tap water; triacylglycerols; tumor necrosis factor-alpha; weight loss
Abstract:
... Spexin (SPX) is a novel adipokine related to many metabolic effects, such as gastrointestinal movements, insulin and glucose homeostasis, lipid metabolism and energy balance. This study evaluates the role of SPX in the improvement of the metabolic and inflammatory profile in fructose-rich-diet obese mice. Adult Swiss mice were supplemented or not with fructose (20% in tap water, FRD and CTR, respe ...
DOI:
10.1016/j.bbalip.2020.158700

91. A canine keratinocyte cell line expresses antimicrobial peptide and cytokine genes upon stimulation with bacteria, microbial ligands and recombinant cytokines

Author:
Suttiwee Chermprapai; Femke Broere; Yvette M. Schlotter; Edwin J.A. Veldhuizen; Victor P.M.G. Rutten
Source:
Veterinary immunology and immunopathology 2018 v.206 pp. 35-40
ISSN:
0165-2427
Subject:
Staphylococcus pseudintermedius; antimicrobial peptides; antimicrobial properties; atopic dermatitis; bacteria; bacterial infections; cell lines; dogs; gene expression; gene expression regulation; genes; interleukins; keratinocytes; ligands; pathogens; receptors; secondary infection; secretion; tumor necrosis factor-alpha
Abstract:
... Keratinocytes (KC) are the main cellular components of the stratum corneum that constitutes a solid physical skin barrier representing the first line of defense against pathogens. Moreover, KC are potent producers of inflammatory mediators and antimicrobial peptides (AMP) when activated through their pattern recognition receptors. In atopic dermatitis (AD) the protective skin barrier may be compro ...
DOI:
10.1016/j.vetimm.2018.11.009

92. Adipocytes harbor a glucosylceramide biosynthesis pathway involved in iNKT cell activation

Author:
Maryam Rakhshandehroo; Robert J. van Eijkeren; Tanit L. Gabriel; Colin de Haar; Sanne M.W. Gijzel; Nicole Hamers; Maria J. Ferraz; Johannes M.F.G. Aerts; Henk S. Schipper; Marco van Eijk; Marianne Boes; Eric Kalkhoven
Source:
Biochimica et biophysica acta 2019 v.1864 no.8 pp. 1157-1167
ISSN:
1388-1981
Subject:
adipocytes; adipose tissue; antigen-presenting cells; antigens; biochemical pathways; biosynthesis; cell communication; cell lines; ceramide glucosyltransferase; ceramides; coculture; energy; glucosylceramides; homeostasis; insulin resistance; interleukin-4; metabolites; secretion
Abstract:
... Natural killer T (NKT) cells in adipose tissue (AT) contribute to whole body energy homeostasis.Inhibition of the glucosylceramide synthesis in adipocytes impairs iNKT cell activity.Glucosylceramide biosynthesis pathway is important for endogenous lipid antigen activation of iNKT cells in adipocytes.Unraveling adipocyte-iNKT cell communication may help to fight obesity-induced AT dysfunction.Overp ...
DOI:
10.1016/j.bbalip.2019.04.016

93. Analysis of intracellular IgG secretion in Chinese hamster ovary cells to improve IgG production

Author:
Kohei Kaneyoshi; Keiji Uchiyama; Masayoshi Onitsuka; Noriko Yamano; Yuichi Koga; Takeshi Omasa
Source:
Journal of bioscience and bioengineering 2019 v.127 no.1 pp. 107-113
ISSN:
1389-1723
Subject:
Chinese hamsters; animal ovaries; antibodies; antibody formation; biopharmaceuticals; cell culture; cell lines; endoplasmic reticulum; engineering; gel chromatography; immunoglobulin G; microscopy; secretion
Abstract:
... The production of biopharmaceutical immunoglobulin G (IgG) using cultured mammalian cells, especially Chinese hamster ovary (CHO) cells is well established and has been markedly improved through the modification of cells and cell culture engineering technologies. The establishment of high-production cell lines remains a challenge. The intracellular secretion of IgG has been investigated to identif ...
DOI:
10.1016/j.jbiosc.2018.06.018

94. Anti-inflammatory activity of dimethyl octenol and oleanene tetrol isolated from Trianthema decandra L.

Author:
Geethalakshmi Rajarathinam; Sarada V. L. Dronamraju
Source:
Molecular biology reports 2018 v.45 no.4 pp. 547-561
ISSN:
0301-4851
Subject:
Fourier transform infrared spectroscopy; Trianthema; animal models; animals; anti-inflammatory activity; catalase; cell lines; chloroform; collagen; computer simulation; glutathione peroxidase; high performance liquid chromatography; inflammation; mass spectrometry; mechanism of action; nitric oxide; nuclear magnetic resonance spectroscopy; octenol; peroxidase; phospholipase A2; phospholipases; prostaglandin synthase; protein denaturation; rheumatoid arthritis; secretion; spectral analysis; therapeutics; tumor necrosis factor-alpha
Abstract:
... Dimethyl octenol from chloroform extract and oleanene tetrol from water extract of Trianthema decandra (TD) were isolated and characterized by using HPLC, UV, FT-IR, NMR, LC–MS and CHNS, their structure were elucidated from their respective spectral data. The anti-inflammatory activity of chloroform extract, water extract, dimethyl octenol and oleanene tetrol of T. decandra were studied and underl ...
DOI:
10.1007/s11033-018-4193-9

95. Anti-inflammatory effects of α-linolenic acid in M1-like macrophages are associated with enhanced production of oxylipins from α-linolenic and linoleic acid

Author:
Samantha D Pauls; Lisa A Rodway; Tanja Winter; Carla G Taylor; Peter Zahradka; Harold M Aukema
Source:
Journal of nutritional biochemistry 2018 v.57 pp. 121-129
ISSN:
0955-2863
Subject:
anti-inflammatory activity; lipopolysaccharides; secretion; noninsulin-dependent diabetes mellitus; obesity; prostaglandins; cell lines; metabolites; linoleic acid; phenotype; macrophages; insulin resistance; omega-3 fatty acids; anti-inflammatory agents; humans; linoleate 13S-lipoxygenase; tumor necrosis factor-alpha; oxylipins; docosahexaenoic acid; eicosapentaenoic acid; alpha-linolenic acid; inflammation; interleukin-6
Abstract:
... Chronic inflammation, mediated in large part by proinflammatory macrophage populations, contributes directly to the induction and perpetuation of metabolic diseases, including obesity, insulin resistance and type 2 diabetes. Polyunsaturated fatty acids (PUFAs) can have profound effects on inflammation through the formation of bioactive oxygenated metabolites called oxylipins. The objective of this ...
DOI:
10.1016/j.jnutbio.2018.03.020

96. Astaxanthin Inhibits Alcohol-induced Inflammation and Oxidative Stress in Macrophages (P02-014-19)

Author:
Hyunju Kang; Yoojin Lee; Minkyung Bae; Myung Joo Han; Young-Ki Park; Ji-Young Lee
Source:
Current developments in nutrition 2019 v.3 no.Supplement_1 pp. -
ISSN:
2475-2991
Subject:
NAD(P)H oxidase (H2O2-forming); NADP (coenzyme); Western blotting; alcohol drinking; anti-inflammatory activity; antioxidant genes; astaxanthin; cell lines; ethanol; gene expression; inflammation; interleukin-1beta; interleukin-6; lipopolysaccharides; lutein; macrophages; messenger RNA; mice; oxidative stress; protein content; proteins; quantitative polymerase chain reaction; reactive oxygen species; secretion; tumor necrosis factor-alpha
Abstract:
... Macrophages play crucial roles in the development of alcohol-induced inflammation. We previously demonstrated the inhibitory effects of astaxanthin (ASTX), a xanthophyll carotenoid, on the expression of pro-inflammatory and antioxidant genes as well as cellular accumulation of reactive oxygen species (ROS) in macrophages stimulated with lipopolysaccharides. The objective of this study was to deter ...
DOI:
10.1093/cdn/nzz029.P02-014-19

97. Attenuation of Atherosclerosis by Protocatechuic Acid via Inhibition of M1 and Promotion of M2 Macrophage Polarization (P04-063-19)

Author:
Yao Liu; Ling Wenhua
Source:
Current developments in nutrition 2019 v.3 no.Supplement_1 pp. -
ISSN:
2475-2991
Subject:
RNA-directed DNA polymerase; Western blotting; anti-inflammatory activity; arginine; atherosclerosis; body weight; cell lines; flavonols; flow cytometry; fluorescent antibody technique; high fat diet; in vitro studies; inflammation; interferon-gamma; interleukin-4; lipopolysaccharides; macrophages; males; mice; nitric oxide synthase; peroxisome proliferator-activated receptor gamma; phosphorylation; protocatechuic acid; secretion; transcription factor NF-kappa B
Abstract:
... Macrophage polarization has a vital impact on the progression of atherosclerosis (AS). Protocatechuic acid (PCA), a flavonol, displays notable atheroprotective effects, but its mechanisms have not been clearly defined. Therefore, we investigated whether PCA attenuated AS by regulating macrophage polarization. Male apolipoprotein E-deficient (ApoE⁻/⁻) mice fed a normal chow diet (NCD), a high-chole ...
DOI:
10.1093/cdn/nzz051.P04-063-19

98. Comparison of human monocyte derived macrophages and THP1-like macrophages as in vitro models for M. tuberculosis infection

Author:
Abhilasha Madhvi; Hridesh Mishra; GR Leisching; PZ Mahlobo; B Baker
Source:
Comparative immunology, microbiology, and infectious diseases 2019 v.67 pp. 101355
ISSN:
0147-9571
Subject:
Mycobacterium tuberculosis; cell lines; chemokines; drug resistance; humans; in vitro studies; macrophages; messenger RNA; models; monocytes; mycobacterial diseases; quantitative polymerase chain reaction; secretion; toxicity testing; viability
Abstract:
... Macrophages are the preferential cell types to study various aspects of mycobacterial infection. Commonly used infection models for in-vitro studies are primary macrophages such as human monocyte derived macrophages (hMDMs) and macrophage like cell lines (THP-1). It is not clear if commercially available THP-1 cells can be used as hMDMs alternative for in-vitro M.tb infection experiments. We condu ...
DOI:
10.1016/j.cimid.2019.101355

99. Compositional Changes Among Triglycerides and Phospholipids During FATP4 Sensitization with Palmitate Lead to ER Stress in Cultured Cells

Author:
Jessica Seessle; Gerhard Liebisch; Gerd Schmitz; Wolfgang Stremmel; Walee Chamulitrat
Source:
European journal of lipid science and technology 2019 v.121 no.5 pp. e1800394
ISSN:
1438-7697
Subject:
blood; cell lines; cholesterol; cholesteryl esters; fatty acid transport proteins; fatty acids; fatty liver; gene expression regulation; genes; low density lipoprotein; mitochondrial membrane; mitogen-activated protein kinase; obesity; palmitates; pathogenesis; phosphatidylcholines; phosphatidylethanolamines; phosphatidylserines; secretion; triacylglycerols
Abstract:
... FATP4 expression is upregulated in acquired obesity and polymorphisms in the FATP4 gene are associated with increased blood triglycerides (TG). Since non‐alcoholic fatty liver disease (NAFLD) is a manifestation of obesity, it is of interest to understand how FATP4 upon fat overload mediates alterations of lipids that lead to pathogenesis of fatty liver. A model system with FATP4‐overexpressed (FAT ...
DOI:
10.1002/ejlt.201800394

100. Curcumin inhibition of the effects of Tip α induced cytokine expression in gastric cancer patients

Author:
Pattama Wongsirisin; Supachai Yodkeeree; Sirikan Limpakan (Yamada); Pornngarm Limtrakul (Dejkriengkraikul)
Source:
PharmaNutrition 2018 v.6 no.3 pp. 100-106
ISSN:
2213-4344
Subject:
Helicobacter pylori; Western blotting; cell lines; chemoprevention; curcumin; dose response; enzyme-linked immunosorbent assay; immunocytochemistry; interleukin-6; interleukin-8; neoplasm cells; patients; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; risk factors; secretion; signal transduction; stomach neoplasms; transcription factor NF-kappa B; tumor necrosis factor-alpha
Abstract:
... Helicobacter pylori (H. pylori) is a major risk factor for gastric cancer. TNF-α inducing protein (Tip α) is an inflammatory factor of H. pylori. It can induce inflammatory cytokine secretion via the NF-kB signaling pathway. The aim of this study is to investigate whether curcumin, a natural NF-kB inhibitor, can reverse the effects of Tip α-induced cytokine expression in gastric cancer subjects bo ...
DOI:
10.1016/j.phanu.2018.05.003