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1. "Healthier" dairy fat using trans-vaccenic acid

Author:
Jayan, G.C.; Herbein, J.H.
Source:
Nutrition and food science 2000 v.30 no.6 pp. 304-309
ISSN:
0034-6659
Subject:
dairy cows; biosynthesis; long chain fatty acids; epithelium; monounsaturated fatty acids; milk fat; acetyl-CoA carboxylase; cell lines; lipid metabolism; stearoyl-CoA desaturase; udders; acyltransferases
DOI:
10.1108/00346650010352924

2.  Elimination of mycoplasma contamination of virus stocks

Author:
H. Malenovska; M. Reichelova
Source:
Veterinární medicína v.56 no.11 pp. 547-550
ISSN:
0375-8427
Subject:
Mycoplasma; analytical kits; antibiotics; cell lines; filters; viral contamination; viruses
Abstract:
... We studied the effectivity of a commercial antibiotic kit Mycokill AB for the elimination of mycoplasma contamination in virus strains. The contaminated virus strains were first filtered, treated with Mycokill AB for three hours and repeatedly passaged in its presence in the cultivation medium in pure cell lines. Three passages in the presence of Mycokill AB were invariably followed by three passa ...
DOI:
10.17221/4273-vetmed

3. (-)-Epigallocatechin-3-gallate and hydroxytyrosol improved antioxidative and anti-inflammatory responses in bovine mammary epithelial cells

Author:
L. Basiricò; P. Morera; D. Dipasquale; R. Bernini; L. Santi; A. Romani; N. Lacetera; U. Bernabucci
Source:
Animal 2019 v.13 no.12 pp. 2847-2856
ISSN:
1751-732X
Subject:
anti-inflammatory activity; antioxidant activity; biomarkers; cell lines; dairy cattle; epigallocatechin gallate; epithelial cells; epithelium; feed supplements; gene expression; glutathione; green tea; hydrogen peroxide; inflammation; interleukin-10; interleukin-6; ligases; lipopolysaccharides; malondialdehyde; mammary glands; messenger RNA; olive oil; oxidative stress; parturition; phenol
Abstract:
... (-)-Epigallocatechin-3-gallate (EGCG), the major phenolic compound of green tea, and hydroxytyrosol (HTyr), a phenol found in olive oil, have received attention due to their wide-ranging health benefits. To date, there are no studies that report their effect in bovine mammary gland. Therefore, the aim of this study was to evaluate the anti-oxidative and anti-inflammatory effects of EGCG and HTyr i ...
DOI:
10.1017/S1751731119001356

4. (2E,9E)-Pyronaamidine 9-(N-methylimine), a new imidazole alkaloid from the Northern Mariana Islands sponge Leucetta sp. cf. chagosensis

Author:
Plubrukarn, A.; Smith, D.W.; Cramer, R.E.; Davidson, B.S.
Source:
Journal of natural products 1997 v.60 no.7 pp. 712-715
ISSN:
0163-3864
Subject:
chemical composition; imidazole alkaloids; spectral analysis; chemical structure; antineoplastic agents; cell lines; Mariana Islands
DOI:
10.1021/np960673o
PubMed:
9249976

5. The (2′S,7′S)-O-(2-methylbutanoyl)-columbianetin as a novel allergic rhinitis-control agent

Author:
Sun-Young Nam; Min-Ho Kim; Youngwan Seo; Youngjin Choi; Jae-Bum Jang; In-Cheol Kang; Myong-Jo Kim; Sok Cheon Pak; Hyung-Min Kim; Hyun-Ja Jeong
Source:
Life sciences 2014 v.98 no.2 pp. 103-112
ISSN:
0024-3205
Subject:
Corydalis; animal models; biomarkers; cell lines; cytokines; eosinophils; gene expression; histamine; immunoglobulin E; intercellular adhesion molecule-1; mast cells; messenger RNA; mice; mitogen-activated protein kinase; nasal mucosa; ovalbumin; pseudopodia; rhinitis; secondary metabolites; signal transduction; therapeutics; traditional medicine; transcription factor NF-kappa B; tryptase; Korean Peninsula
Abstract:
... The (2′S,7′S)-O-(2-methylbutanoyl)-columbianetin (OMC) is a novel secondary metabolite extracted from Corydalis heterocarpa, which has long been used as a folk medicine for various inflammatory diseases in Korea. We examined the effect of OMC on allergic rhinitis (AR).We assessed the therapeutic effects and regulatory mechanisms of OMC on the phorbol 12-myristate 13-acetate plus A23187-stimulated ...
DOI:
10.1016/j.lfs.2014.01.003

6. (Cyto)genomic and epigenetic characterization of BICR 10 cell line and three new established primary human head and neck squamous cell carcinoma cultures

Author:
Ilda P. Ribeiro; Joana M. Rodrigues; Alexandra Mascarenhas; Vanessa Marques; Francisco Caramelo; Maria J. Julião; Thomas Liehr; Joana B. Melo; Isabel M. Carreira
Source:
Genes & genomics 2019 v.41 no.10 pp. 1207-1221
ISSN:
1976-9571
Subject:
GATA transcription factors; cell culture; cell lines; chromosome translocation; chromosomes; comparative genomic hybridization; epigenetics; fluorescence in situ hybridization; genes; genomics; head and neck neoplasms; humans; karyotyping; methylation; models; neoplasm cells; squamous cell carcinoma; therapeutics
Abstract:
... BACKGROUND: Head and neck squamous cell carcinoma cell lines are useful preclinical models to understand the molecular processes underlying the development of such tumors, and to establish targeted therapies. OBJECTIVE: We performed a comprehensive (cyto)genomic and epigenetic characterization of three new established primary human head and neck squamous cell carcinoma cultures and an established, ...
DOI:
10.1007/s13258-019-00850-6

7. (R)-3-(8'-Hydroxyfarnesyl)-indole and other chemical constituents from the flowers of Anomianthus dulcis and their antimalarial and cytotoxic activities

Author:
Thanika Promchai; Thanaphat Thaima; Roonglawan Rattanajak; Sumalee Kamchonwongpaisan; Stephen G. Pyne; Thunwadee Limtharakul
Source:
Natural product research 2021 v.35 no.15 pp. 2476-2481
ISSN:
1478-6427
Subject:
antimalarials; cell lines; chalcone; chromatography; cytotoxicity; flavanones; flavones; research; spectroscopy
Abstract:
... A new farnesylindole, (R)-3-(8'-hydroxyfarnesyl)-indole (1), as a scalemic mixture (33% ee) along with nine known compounds (2-10), including one farnesylindole, three flavanones, three flavone derivatives and two chalcone derivatives were isolated from the methanolic crude extract of the flowers from Anomianthus dulcis. All compounds were purified by appropriate chromatographic techniques and the ...
DOI:
10.1080/14786419.2019.1679139

8. (R)‐Tonkafuranone and related compounds: Improved synthesis, stereochemical purity in nature, and bioactivities of the pure enantiomers

Author:
Matthias Hoffmann; Jean‐Pierre Mufusama Koy Sita; Carolin Kleider; Sandra Gunesch; Bruno Rothmund; Leane Lehmann; Gerhard Bringmann; Michael Decker
Source:
Flavour and fragrance journal 2019 v.34 no.5 pp. 329-338
ISSN:
0882-5734
Subject:
Bursera; Lewis bases; cell lines; condensation reactions; cytotoxicity; enantiomers; flavor compounds; high performance liquid chromatography; lactones; mice; moieties; mutagens; patulin; purification methods; stereochemistry
Abstract:
... Dehydromenthofurolactone (tonkafuranone) and other bicyclic lactones are common flavour compounds and can be found in a variety of plants. Herein, we present an improved synthesis of the natural product and a series of close analogues, in combination with efficient purification methods for simple preparation from cheap and easily accessible chemicals. A two‐step, one‐pot, reaction procedure, consi ...
DOI:
10.1002/ffj.3513

9. (Z)-sarcodictyin A, a new highly cytotoxic diterpenoid from the soft coral Bellonella albiflora

Author:
Nakao, Y.; Yoshida, S.; Matsunaga, S.; Fusetani, N.
Source:
Journal of natural products 2003 v.66 no.4 pp. 524-527
ISSN:
0163-3864
Subject:
corals; animal products; diterpenoids; cytotoxins; chemical structure; stereochemistry; spectral analysis; olefin; protons; humans; cell lines; cytotoxicity; Japan
DOI:
10.1021/np0205452
PubMed:
12713406

10. 065 Designing potent inhibitors of Ice recrystallization - from C-linked antifreeze glycoproteins (AFGPs) to small molecules

Author:
Robert Ben
Source:
Cryobiology 2013 v.67 no.3 pp. 416
ISSN:
0011-2240
Subject:
amino acids; antifreeze proteins; carbon; cell lines; cell viability; cryopreservation; cryoprotectants; crystallization; crystals; death; dimethyl sulfoxide; encapsulation; fish; glycerol; glycoconjugates; glycosides; health services; hysteresis; ice; mechanical damage; models; peptides; sowing; stem cells; surfactants; therapeutics; viruses
Abstract:
... Cell-based therapeutics has emerged as a critical aspect of modern health care and has shaped new models of care in stem cell and regenerative therapy. Dimethyl sulfoxide and glycerol are the two most common cryoprotectants. Unfortunately, many of the current cryopreservation protocols are suboptimal resulting in poor cell recoveries and/or compromised cellular function post cryopreservation thus ...
DOI:
10.1016/j.cryobiol.2013.09.071

11. 1',2',3',4'-Tetradehydrotubulosine, a cytotoxic alkaloid from Pogonopus speciosus

Author:
Ito, A.; Lee, Y.H.; Chai, H.B.; Gupta, M.P.; Farnsworth, N.R.; Cordell, G.A.; Pezzuto, J.M.; Kinghorn, A.D.
Source:
Journal of natural products 1999 v.62 no.9 pp. 1346-1348
ISSN:
0163-3864
Subject:
Rubiaceae; stems; alkaloids; spectral analysis; chemical structure; antineoplastic agents; humans; cell lines; medicinal properties; chemical constituents of plants; Panama
DOI:
10.1021/np990255u
PubMed:
10514334

12. 1,6-heptadiynes based cyclopolymerization functionalized with mannose by post polymer modification for protein interaction

Author:
Pawan Kumar; Pintu Kanjilal; Rituparna Das; Tapan K. Dash; Manikandan Mohanan; Trong-Nghia Le; N. Vijayakameswara Rao; Balaram Mukhopadhyay; Raja Shunmugam
Source:
Carbohydrate research 2021 v.508 pp. 108397
ISSN:
0008-6215
Subject:
catalysts; cell lines; cell viability; chromatography; cytotoxicity; fluorescence; malonic acid; mannose; mass spectrometry; moieties; polymers; research
Abstract:
... Carbohydrate functionalized polymers or Glycopolymers have earned a great deal of interest in recent times for their potential biomedical applications. In the present study, a mannose containing glycopolymer was synthesized by cyclopolymerization of malonic acid derivative using second generation Hoveyda Grubbs′ catalyst. Post-polymerization modification was done to install a propargyl moiety. Fin ...
DOI:
10.1016/j.carres.2021.108397

13. 1,7‐Bis(4‐hydroxyphenyl)‐4‐hepten‐3‐one from Betula platyphylla induces apoptosis by suppressing autophagy flux and activating the p38 pathway in lung cancer cells

Author:
Hyun Jin Jung; Kyung‐Sik Song; Youn Kyoung Son; Je Kyung Seong; Sun Yeou Kim; Seung Hyun Oh
Source:
Phytotherapy research 2020 v.34 no.1 pp. 126-138
ISSN:
0951-418X
Subject:
Betula pendula subsp. mandshurica; antineoplastic agents; apoptosis; autophagy; cell cycle checkpoints; cell lines; cell viability; drug therapy; human diseases; lung neoplasms; mitosis; neoplasm cells; protein synthesis; rapamycin
Abstract:
... Betula platyphylla (BP) is frequently administered in the treatment of various human diseases, including cancers. This study was undertaken to investigate the pharmacological function of the active components in BP and the underlying mechanism of its chemotherapeutic effects in human lung cancer cells. We observed that BP extracts and 1,7‐bis(4‐hydroxyphenyl)‐4‐hepten‐3‐one (BE1), one of the compo ...
DOI:
10.1002/ptr.6506

14. 1-Butylimidazole-derived ionic liquids: synthesis, characterisation and evaluation of their antibacterial, antifungal and anticancer activities

Author:
Prabodh Ranjan; Bheru Singh Kitawat; Man Singh
Source:
RSC advances 2014 v.4 no.96 pp. 53634-53644
ISSN:
2046-2069
Subject:
Bacillus subtilis; Escherichia coli; Fourier transform infrared spectroscopy; Pseudomonas aeruginosa; Staphylococcus aureus; antifungal properties; antineoplastic activity; carbon; cell growth; cell lines; doxorubicin; ionic liquids; nuclear magnetic resonance spectroscopy; organic halogen compounds; stable isotopes
Abstract:
... A series of new 1-butylimidazole-based ionic liquids (3a–h) have been synthesised by the quaternisation reaction of 1-butylimidazole with different alkyl- and alkoxy-substituted aryl halides using a microwave solvent-free approach with ≈82–95% yield. The solvent-free approach allows the preparation of a variety of ILs with better yields and purities, making any further purification unnecessary. Th ...
DOI:
10.1039/c4ra08370a

15. 1-Deoxynojirimycin Inhibits Metastasis of B16F10 Melanoma Cells by Attenuating the Activity and Expression of Matrix Metalloproteinases-2 and -9 and Altering Cell Surface Glycosylation

Author:
Wang, Ran-Juh; Yang, Chun-Hung; Hu, Miao-Lin
Source:
Journal of agricultural and food chemistry 2010 v.58 no.16 pp. 8988-8993
ISSN:
0021-8561
Subject:
messenger RNA; cell movement; melanoma; metalloproteinases; glycosylation; mulberries; flow cytometry; mannose; metastasis; enzyme inhibitors; cell membranes; gene expression; binding sites; cell lines; enzyme inhibition; cell adhesion; anticarcinogenic activity
Abstract:
... 1-Deoxynojirimycin (1-DNJ), an iminosugar rich in mulberry, has been shown to possess antimetastatic potential. The antimetastatic mechanisms of 1-DNJ in melanoma B16F10 cells were studied, as were the antimetastatic activity (cell adhesion, migration, and invasion) of 1-DNJ, matrix metalloproteinases (MMP-2 and MMP-9), tissue inhibitors of metalloproteinase (TIMP-1 and TIMP-2) mRNA, and flow cyto ...
DOI:
10.1021/jf101401b
PubMed:
23654233

16. 105 Akt signaling mediates prostate cancer response to cryoablation

Author:
John M. Baust; Daniel Klossner; Andrew Gage; Robert Van Buskirk; John G. Baust
Source:
Cryobiology 2013 v.67 no.3 pp. 427-428
ISSN:
0011-2240
Subject:
Western blotting; androgen receptors; apoptosis; cell lines; cell viability; cryosurgery; freezing; men; mitogen-activated protein kinase; patients; prostatic neoplasms; quality of life; remission; signal transduction; United States
Abstract:
... Prostate cancer (CaP) has a significant impact on men’s health and quality of life with more than 230,000 new cases diagnosed annually in the United States. The androgen signaling pathway is integral in CaP progression and as such androgen deprivation is a widely utilized therapeutic option to treat CaP. Typically, with the application of the anti androgen treatment, the androgen sensitive (AS) Ca ...
DOI:
10.1016/j.cryobiol.2013.09.111

17. 11-Deoxyfistularin-3, a new cytotoxic metabolite from the Caribbean sponge Aplysina fistularis insularis

Author:
Compagnone, R.S.; Avila, R.; Suarez, A.I.; Abrams, O.V.; Rangel, H.R.; Arvelo, F.; Pina, I.C.; Merentes, E.
Source:
Journal of natural products 1999 v.62 no.10 pp. 1443-1444
ISSN:
0163-3864
Subject:
chemical composition; bromine; tyrosine; metabolites; spectral analysis; chemical structure; antineoplastic agents; humans; carcinoma; breast neoplasms; cell lines; Venezuela
DOI:
10.1021/np9901938
PubMed:
10543914

18. 110ᵗʰ Anniversary: Fast and Easy-to-Use Method for Coating Tissue Culture Polystyrene Surfaces with Nonfouling Copolymers To Prevent Cell Adhesion

Author:
Nicolò Manfredini; Ernesto Scibona; Massimo Morbidelli; Davide Moscatelli; Mattia Sponchioni
Source:
Industrial & engineering chemistry process design and development 2019 v.58 no.49 pp. 22290-22298
ISSN:
1520-5045
Subject:
adhesion; adsorption; cell adhesion; cell lines; coatings; composite polymers; hydrophilicity; moieties; polystyrenes; prices; tissue culture
Abstract:
... Synthetic substrates able to prevent the adhesion of cells, commonly referred to as nonfouling surfaces, are useful in a wide range of applications, including the culture of multicellular spheroids and the engineering of complex cell sheets. Nonfouling properties are commonly conferred to surfaces through superhydrophilic polymer-based coatings. At the same time, this type of treatment requires di ...
DOI:
10.1021/acs.iecr.9b05104

19. 118 Semen quality and fertilization ability of myostatin-knockout boars

Author:
M.-F. Xuan; S.-Z. Han; B.-H. Quan; X.-J. Yin; J.-D. Kang
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 186
ISSN:
1031-3613
Subject:
Duroc; acrosome; blastocyst; boars; cell lines; color; ejaculation; genotype; hybrids; in vitro fertilization; mitochondria; odors; oocytes; pH; phenotype; piglets; plasma membrane; pork; progeny; propidium; reproductive performance; semen quality; sexual maturity; sires; somatic cells; sows; staining; t-test
Abstract:
... Myostatin-knockout (MSTN−/−) pigs may provide a source of healthy lean protein for human consumption. However, little is known about the effect of this knockout on semen quality, which is important if these pigs are used for production. The purpose of this study is to evaluate the semen quality and fertility of MSTN−/− boars. We generated MSTN−/− boars from Duroc-Landrace-Yorkshire hybrid pig cell ...
DOI:
10.1071/RDv32n2Ab118

20. 12-Epi-9-deacetoxyxenicin, new cytotoxic diterpenoid from a Bornean soft coral, Xenia sp.

Author:
Chin-Soon Phan; Takashi Kamada; Takahiro Ishii; Toshiyuki Hamada; Charles Santhanaraju Vairappan
Source:
Natural product research 2019 v.33 no.6 pp. 808-813
ISSN:
1478-6427
Subject:
Lagenidium; cell lines; corals; cytotoxicity; diterpenoids; hyphae; secondary metabolites; sesquiterpenoids; spectral analysis; Borneo
Abstract:
... One new compound, 12-epi-9-deacetoxyxenicin (1) along with a hydroperoxide product, 12-epi-9-deacetoxy-8-hydroperoxyxenicin (2) and two known sesquiterpenoids (3–4) were isolated from a population of Bornean soft coral Xenia sp. The structures of these secondary metabolites were elucidated based on their spectroscopic data. Compounds 1 and 2 showed cytotoxic activity against ATL cell line, S1T. In ...
DOI:
10.1080/14786419.2017.1410812