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1. Characterization of pIgR and its association with resistance to iridovirus in Asian seabass

Author:
Yi Xuan Tay; Yepin Yu; Zituo Yang; Le Wang; Fei Sun; Gen Hua Yue
Source:
Aquaculture 2023 v.562 pp. 738783
ISSN:
0044-8486
Subject:
Lates calcarifer; Singapore grouper iridovirus; aquaculture; cell lines; coat proteins; disease resistance; eyes; fingerlings; genes; genotyping; heart; immunoglobulins; intestines; kidneys; mucosal immunity; polymers
Abstract:
... The improvement of disease resistance is vital in aquaculture as diseases are one of the serious threats to successful aquaculture. Understanding of molecular mechanisms underlying disease resistance is important in improving resistance against diseases. The pIgR (polymeric immunoglobulin receptor) gene plays an important role in the mucosal immunity of fish. However, not much is known about its r ...
DOI:
10.1016/j.aquaculture.2022.738783

2. Diagnostic kit based on halloysite nanoclay-ionic liquid nanocomposite modified electrode for electrochemical determination of cancer biomarker

Author:
Esma Yildiz; Beste Yurdacan; Yasemin Erac; Arzum Erdem
Source:
Talanta 2023 v.252 pp. 123854
ISSN:
0039-9140
Subject:
DNA probes; RNA; analytical kits; biomarkers; breast neoplasms; carbon electrodes; cell lines; detection limit; dielectric spectroscopy; electrochemistry; energy-dispersive X-ray analysis; halloysite; humans; hybridization; inosine; kidneys; liquids; nanocomposites; neoplasm cells; nucleic acid hybridization; nucleotide sequences; voltammetry
Abstract:
... Nucleic acid hybridization is occurred between the selective single-stranded nucleic acid sequence and its target sequence, which is one of the essential procedure for electrochemical detection of nucleic acid. microRNA-21 (miRNA-21) is known as a biomarker in various cancers. The determination of miRNA-21 was attained through by hybridization of inosine substituted miRNA-21 specific DNA probe (Pᵢ ...
DOI:
10.1016/j.talanta.2022.123854

3. Establishment and characterization of a cell line from the brain of the Japanese flounder (Paralichthys olivaceus) and its application in the study of viral infection

Author:
Zhaodi Sun; Yuqin Ren; Yitong Zhang; Yucong Yang; Guixing Wang; Zhongwei He; Yufeng Liu; Wei Cao; Yufen Wang; Yuanshuai Fu; Jilun Hou
Source:
Aquaculture 2023 v.562 pp. 738825
ISSN:
0044-8486
Subject:
Andrias davidianus; Hirame rhabdovirus; Iridovirus; Paralichthys olivaceus; actin; aquaculture; brain; cell lines; chromosome number; chromosomes; diploidy; fish; gene expression; genes; host-pathogen relationships; interleukin-8; karyotyping; neural stem cells; temperature; transfection; viruses
Abstract:
... Fish cell lines are important tools in many biological studies. Here, we established a new Japanese flounder brain (JFB) cell line from the brain tissue of the Japanese flounder (Paralichthys olivaceus), which was successfully sub-cultured for over 120 passages, and consisted predominantly of fibroblast-like cells. Sequencing of the actin gene confirmed that JFB cells were derived from the Japanes ...
DOI:
10.1016/j.aquaculture.2022.738825

4. Fate and PPARγ and STATs-driven effects of the mitochondrial complex I inhibitor tebufenpyrad in liver cells revealed with multi-omics

Author:
Thibaut Léger; Patrick Balaguer; Ludovic Le Hégarat; Valérie Fessard
Source:
Journal of hazardous materials 2023 v.442 pp. 130083
ISSN:
0304-3894
Subject:
beta oxidation; cell culture; cell lines; dose response; hepatocytes; humans; inflammation; interleukin-6; liver; metabolites; metabolomics; mitochondria; multiomics; oxidative phosphorylation; pesticides; proteasome endopeptidase complex; proteomics; rats; secretion; transcription factors
Abstract:
... The biological effects of the pesticide and mitochondrial complex I inhibitor tebufenpyrad (TEBU) on liver cells were investigated by combining proteomics and metabolomics. Both cell culture media and cellular lysates were analyzed in dose-response and kinetic experiments on the HepaRG cell line. Responses were compared with those obtained on primary human and rat hepatocytes. A multitude of phase ...
DOI:
10.1016/j.jhazmat.2022.130083

5. Food peptides as inducers of CCK and GLP-1 secretion and GPCRs involved in enteroendocrine cell signalling

Author:
Marta Santos-Hernández; Santiaga María Vivanco-Maroto; Beatriz Miralles; Isidra Recio
Source:
Food chemistry 2023 v.402 pp. 134225
ISSN:
0308-8146
Subject:
G-proteins; calcium receptors; cell lines; cholecystokinin; digestion; food chemistry; gastrointestinal system; glucagon-like peptide 1; hormone secretion; lysozyme
Abstract:
... The strong effect of protein digestion products on gastrointestinal-released hormones is recognised. However, little is known about the specific peptide sequences able to induce gastrointestinal hormone secretion and the receptors involved. Our objective was to identify peptides able to induce the secretion of cholecystokinin (CCK) and glucagon like peptide-1 (GLP-1) in the enteroendocrine cell li ...
DOI:
10.1016/j.foodchem.2022.134225

6. Metabolomics driven analysis of Nigella sativa seeds identifies the impact of roasting on the chemical composition and immunomodulatory activity

Author:
Mohamed A. Salem; Riham A. El-Shiekh; Nora M. Aborehab; Ahmed A. Al‐Karmalawy; Shahira M. Ezzat; Saleh Alseekh; Alisdair R. Fernie
Source:
Food chemistry 2023 v.398 pp. 133906
ISSN:
0308-8146
Subject:
Nigella sativa; cell lines; chemical composition; flavonoids; flavor; food chemistry; immunomodulators; metabolites; metabolomics; monocytes; saponins
Abstract:
... Nigella sativa L. seeds (NS) are known as one of the most traditional immunomodulatory and nutritive food additives. NS can, furthermore, be roasted to give curries, breads, and other dishes a smoky, nutty flavor. This study evaluated the effect of roasting NS on the metabolic profile and immunomodulatory activity. Non-targeted metabolomics analysis was conducted using several analytical platforms ...
DOI:
10.1016/j.foodchem.2022.133906

7. N-acetylation of toxic aromatic amines by fungi: Strain screening, cytotoxicity and genotoxicity evaluation, and application in bioremediation of 3,4-dichloroaniline

Author:
Amanda Dal’Ongaro Rodrigues; Arthur dos Santos Montanholi; Angela Akimi Shimabukuro; Murilo Kioshi Aquino Yonekawa; Nadla Soares Cassemiro; Denise Brentan Silva; Clarice Rossato Marchetti; Carlos Eduardo Weirich; Adilson Beatriz; Fabiana Fonseca Zanoelo; Maria Rita Marques; Giovana Cristina Giannesi; Silvia Cordeiro das Neves; Rodrigo Juliano Oliveira; Roberto Ruller; Dênis Pires de Lima; Edson dos Anjos dos Santos
Source:
Journal of hazardous materials 2023 v.441 pp. 129887
ISSN:
0304-3894
Subject:
Aspergillus niveus; Aspergillus terreus; Chaetomium; Cladosporium cladosporioides; Lactuca sativa; Mucor; bioremediation; biotransformation; cell lines; colorimetry; cytotoxicity; fungi; genotoxicity; humans; industry; keratinocytes; mutagens; phenylurea herbicides; phytotoxicity; soil
Abstract:
... Aromatic amines (AA) are one of the most commonly used classes of compounds in industry and the most common pollutants found in both soil and water. 3,4-Dichloaniline (3,4-DCA) is a persistent residue of the phenylurea herbicide in the environment. In this study, we used a colorimetric method as a new approach to screen 12 filamentous fungal strains of the genera Aspergillus, Chaetomium, Cladospor ...
DOI:
10.1016/j.jhazmat.2022.129887

8. 2‐Arachidonoyl glycerol suppresses gastric emptying via the cannabinoid receptor 1‐cholecystokinin signaling pathway in mice

Author:
Keita Ochiai; Rina Hirooka; Masayoshi Sakaino; Shigeo Takeuchi; Tohru Hira
Source:
Lipids 2022 v.57 no.3 pp. 173-181
ISSN:
0024-4201
Subject:
absorption; acetaminophen; antagonists; cannabinoid receptors; cell lines; cholecystokinin; cholecystokinin receptors; glycerol; intraperitoneal injection; mice; phenolsulfonphthalein; secretion
Abstract:
... 2‐Monoacylglycerol (2‐MAG) is one of the digestion products of dietary lipids. We recently demonstrated that a 2‐MAG, 2‐arachidonoyl glycerol (2‐AG) potently stimulated cholecystokinin (CCK) secretion via cannabinoid receptor 1 (CB1) in a murine CCK‐producing cell line, STC‐1. CCK plays a crucial role in suppressing postprandial gastric emptying. To examine the effect of 2‐AG on gastric emptying, ...
DOI:
10.1002/lipd.12341

9. 3,4,5‐O‐tricaffeoylquinic acid alleviates ionizing radiation‐induced injury in vitro and in vivo through regulating ROS/JNK/p38 signaling

Author:
Jiajun Liu; Wen Hu; Xiaoying Ma; Xin Liang; Long Lin; Jianming Huang; Jianwen Liu
Source:
Environmental toxicology 2022 v.37 no.2 pp. 349-361
ISSN:
1520-4081
Subject:
Azolla; Western blotting; agonists; apoptosis; caspases; cell lines; cytotoxicity; digestive system; ecotoxicology; epithelial cells; immune system; membrane potential; mitochondria; radioprotective effect; radiotherapy
Abstract:
... Ionizing radiation (IR) brings many health problems to humans, causing damage to the digestive system, hematopoietic system, and immune system. Natural compounds derived from plants have attracted widespread attention due to their low toxicity. Here, we found that 3,4,5‐O‐tricaffeoylquinic acid (tCQA) extracted from natural plant Azolla imbricata could significantly alleviate the systemic damage i ...
DOI:
10.1002/tox.23403

10. 7-Hydroxy-2-octenoic acid-ethyl ester mixture as an UV protectant secondary metabolite of an endolichenic fungus isolated from Menegazzia terebrata

Author:
Lu Zhao; Jin-Cheol Kim; Jae-Seoun Hur
Source:
Archives of microbiology 2022 v.204 no.7 pp. 395
ISSN:
0302-8933
Subject:
Menegazzia; absorption; ascorbic acid; cell lines; cytotoxicity; fungi; irradiation; linoleic acid; melanin; melanoma; mice; peroxidation; secondary metabolites; superoxide anion
Abstract:
... Enodolichenic fungi (ELF) are considered a promising bio-resource since they produce a variety of novel secondary metabolites with bioactivities. Ultraviolet (UV) radiation in sunlight containing UVA and UVB can cause acute and chronic skin diseases, and the demand for UV protectants in sunscreens has been increasing. Such situations evoke the strong interest of researchers in seeking effective UV ...
DOI:
10.1007/s00203-022-02997-5

11. In vitro transmission of Chlamydia using naturally infected koala (Phascolarctos cinereus) semen

Author:
Lyndal Hulse; Kenneth W. Beagley; Rebecca Larkin; Stephen D. Johnston
Source:
Reproduction, fertility, and development 2022 v.34 no.3 pp. 378-384
ISSN:
1031-3613
Subject:
Chlamydia; Chlamydophila pecorum; Phascolarctos cinereus; cell lines; immune response; quantitative polymerase chain reaction; reproduction; semen
Abstract:
... Transmission of Chlamydia pecorum infection has generally been assumed to be via the urogenital route and in an attempt to confirm this we investigated an in vitro method of Chlamydia infection using naturally infected koala semen to inoculate a cell line and attempt to estimate C. pecorum infectious load. A total of 57% of 122 koala semen samples had low C. pecorum copy number or no burden, while ...
DOI:
10.1071/RD21233

12. Accurate identification of circRNA landscape and complexity reveals their pivotal roles in human oligodendroglia differentiation

Author:
Yangping Li; Feng Wang; Peng Teng; Li Ku; Li Chen; Yue Feng; Bing Yao
Source:
Genome biology 2022 v.23 no.1 pp. 48
ISSN:
1474-760X
Subject:
brain; cell lines; exons; gene expression; humans; introns; landscapes; microRNA; neurons; oligodendroglia; ribonucleases
Abstract:
... BACKGROUND: Circular RNAs (circRNAs), a novel class of poorly conserved non-coding RNAs that regulate gene expression, are highly enriched in the human brain. Despite increasing discoveries of circRNA function in human neurons, the circRNA landscape and function in developing human oligodendroglia, the myelinating cells that govern neuronal conductance, remains unexplored. Meanwhile, improved expe ...
DOI:
10.1186/s13059-022-02621-1

13. Acetate, a metabolic product of Heligmosomoides polygyrus, facilitates intestinal epithelial barrier breakdown in a FFAR2-dependent manner

Author:
Fabian Schälter; Michael Frech; Kerstin Dürholz; Sébastien Lucas; Kerstin Sarter; Luc Lebon; Julia Esser-von Bieren; Lalit K. Dubey; David Voehringer; Georg Schett; Nicola L. Harris; Mario M. Zaiss
Source:
International journal for parasitology 2022 v.52 no.9 pp. 591-601
ISSN:
0020-7519
Subject:
G-proteins; Heligmosomoides polygyrus; acetates; cell lines; energy metabolism; epithelial cells; epithelium; free fatty acids; helminths; intestines; livestock; organoids; parasitology; permeability; reaction kinetics; tight junctions
Abstract:
... Approximately 2 billion people worldwide and a significant part of the domestic livestock are infected with soil-transmitted helminths, of which many establish chronic infections causing substantial economic and welfare burdens. Beside intensive research on helminth-triggered mucosal and systemic immune responses, the local mechanism that enables infective larvae to cross the intestinal epithelial ...
DOI:
10.1016/j.ijpara.2022.04.004

14. Achieving robust somatic mutation detection with deep learning models derived from reference data sets of a cancer sample

Author:
Sayed Mohammad Ebrahim Sahraeian; Li Tai Fang; Konstantinos Karagiannis; Malcolm Moos; Sean Smith; Luis Santana-Quintero; Chunlin Xiao; Michael Colgan; Huixiao Hong; Marghoob Mohiyuddin; Wenming Xiao
Source:
Genome biology 2022 v.23 no.1 pp. 12
ISSN:
1474-760X
Subject:
DNA; DNA damage; cell lines; computer simulation; gene frequency; genome; genomics; models; neoplasm cells; neoplasms; somatic mutation
Abstract:
... BACKGROUND: Accurate detection of somatic mutations is challenging but critical in understanding cancer formation, progression, and treatment. We recently proposed NeuSomatic, the first deep convolutional neural network-based somatic mutation detection approach, and demonstrated performance advantages on in silico data. RESULTS: In this study, we use the first comprehensive and well-characterized ...
DOI:
10.1186/s13059-021-02592-9

15. Acrylamide causes neurotoxicity by inhibiting glycolysis and causing the accumulation of carbonyl compounds in BV2 microglial cells

Author:
Fan Wang; Ben Fan; Chao Chen; Wensheng Zhang
Source:
Food and chemical toxicology 2022 v.163 pp. 112982
ISSN:
0278-6915
Subject:
acrylamides; cell lines; glycolysis; isomerases; lactic acid; mice; models; neuroglia; neurotoxicity; neurotoxins; oxidative stress; oxidoreductases; pyruvate kinase; pyruvic acid; toxicology
Abstract:
... Acrylamide is a potent neurotoxin produced during industrial production or food processing at high temperatures, and its effect on glycolytic processes may be critical in triggering neurotoxicity. Our work investigated the neurotoxic effects of acrylamide based on glycolysis using immortalized mouse microglia cell line BV2. The results showed that 1.5 mM acrylamide significantly inhibited the expr ...
DOI:
10.1016/j.fct.2022.112982

16. The African Swine Fever Isolate ASFV-Kenya-IX-1033 Is Highly Virulent and Stable after Propagation in the Wild Boar Cell Line WSL

Author:
Johanneke D. Hemmink; Hussein M. Abkallo; Sonal P. Henson; Emmanuel M. Khazalwa; Bernard Oduor; Anna Lacasta; Edward Okoth; Victor Riitho; Walter Fuchs; Richard P. Bishop; Lucilla Steinaa
Source:
Viruses 2022 v.14 no.9 pp. -
ISSN:
1999-4915
Subject:
African swine fever; blood; cell lines; genome; genotype; live vaccines; lungs; macrophages; models; swine; virulence; virus replication; viruses; wild boars; Central Africa; Kenya
Abstract:
... We describe the characterization of an African swine fever genotype IX virus (ASFV-Kenya-IX-1033), which was isolated from a domestic pig in western Kenya during a reported outbreak. This includes the efficiency of virus replication and in vivo virulence, together with genome stability and virulence, following passage in blood macrophages and in a wild boar lung cell line (WSL). The ASFV-Kenya-IX- ...
DOI:
10.3390/v14091912

17. Alachlor breaks down intracellular calcium homeostasis and leads to cell cycle arrest through JNK/MAPK and PI3K/AKT signaling mechanisms in bovine mammary gland epithelial cells

Author:
Miji Kim; Garam An; Whasun Lim; Gwonhwa Song
Source:
Pesticide biochemistry and physiology 2022 v.184 pp. 105063
ISSN:
0048-3575
Subject:
alachlor; calcium; cattle; cell cycle checkpoints; cell lines; cell viability; cyclin-dependent kinase; cyclins; epithelial cells; epithelium; homeostasis; mammary glands; membrane potential; milk; mitochondria; mitochondrial membrane; non-specific serine/threonine protein kinase; phosphorylation; toxicity; toxicology
Abstract:
... Alachlor is a widely used herbicide for the cultivation of various grains employed as food for cattle. The mechanisms leading to the toxic effects of alachlor on epithelial cells of the bovine mammary gland are not well known. Thus, this study was conducted to clarify the toxicological effects of alachlor on the immortalized epithelial cell line of the bovine mammary gland (MAC-T) cells. After tre ...
DOI:
10.1016/j.pestbp.2022.105063

18. Aloe gel glucomannan induced colon cancer cell death via mitochondrial damage-driven PINK1/Parkin mitophagy pathway

Author:
Ke Zhang; Duoduo Zhang; Junqiao Wang; Yuting Wang; Jiarui Hu; Yujia Zhou; Xingtao Zhou; Shaoping Nie; Mingyong Xie
Source:
Carbohydrate polymers 2022 v.295 pp. 119841
ISSN:
0144-8617
Subject:
Aloe; antineoplastic agents; cell lines; colorectal neoplasms; cytotoxicity; gels; glucomannans; homeostasis; lysosomes; mitochondria; mitophagy; neoplasm cells; reactive oxygen species; sequence analysis; transcription factors
Abstract:
... Mitophagy can selectively remove damaged mitochondria, which is critical in regulating mitochondrial homeostasis in diseases, such as cancer. Herein, we found that Aloe gel glucomannan (AGP) significantly inhibited the proliferation of colon cancer cells. RNA-seq analysis revealed that AGP upregulated autophagy, lysosome and mitochondrial fission signal pathways in colon cancer cell line CT26. Not ...
DOI:
10.1016/j.carbpol.2022.119841

19. Aloe vera and Fermented Extracts Exhibit an Anti-Inflammatory Effect on Human Glioblastoma/Astrocytoma U373 MG Cells

Author:
Antonio Tornero-Martínez; Maria del Carmen Silva-Lucero; Edén Cano Sampedro; Eva Ramón-Gallegos; Claudia Pérez-Cruz; Brenda Pérez-Grijalva; Rosalva Mora-Escobedo
Source:
Plant foods for human nutrition 2022 v.77 no.1 pp. 37-43
ISSN:
0921-9668
Subject:
Aloe vera; anti-inflammatory activity; astrocytes; cell lines; cell viability; digestion; fermentation; glioblastoma; human nutrition; humans; inflammation; interleukin-6; mitogen-activated protein kinase; polysaccharides; substance P
Abstract:
... The anti-inflammatory effects of Aloe vera (AV), polysaccharide extract from AV, and extracts from the digestion and colonic fermentation of AV were evaluated using an immortal astrocyte cell line (U373 MG) that develops a neuro-inflammatory profile. Cell viability and inflammatory markers were assessed after stimulation with neuropeptide substance P (SP) that activates the pro-inflammatory MAPK ( ...
DOI:
10.1007/s11130-022-00957-4

20. Alpha-crystallin B chains in trastuzumab-resistant breast cancer cells promote endothelial cell tube formation through activating mTOR

Author:
Lili Yang; Kazuma Higashisaka; Masafumi Shimoda; Yuya Haga; Naoki Sekine; Hirofumi Tsujino; Kazuya Nagano; Kenzo Shimazu; Yasuo Tsutsumi
Source:
Biochemical and biophysical research communications 2022 v.588 pp. 175-181
ISSN:
0006-291X
Subject:
adjuvants; angiogenesis; breast neoplasms; cell lines; endothelial cells; erbB-2 receptor; metastasis; monoclonal antibodies; proteomics; rapamycin; relapse; research
Abstract:
... The specific human epidermal growth factor receptor 2 (HER2)-targeting monoclonal antibody trastuzumab shows considerable clinical efficacy in patients with HER2-overexpressing breast cancer. However, about 20% of patients who receive trastuzumab in the adjuvant setting relapse, and approximately half of patients with metastatic HER2-positive breast cancer develop resistance to trastuzumab within ...
DOI:
10.1016/j.bbrc.2021.12.056

21. Alteration of miR-362-5p and miR-454-3p expression elicits diverse responses in breast cancer cell lines

Author:
Raghu Aarthy; Arunagiri Kuha Deva Magendhra Rao; Krishna Patel; Velusami Sridevi; Thangarajan Rajkumar; Harsha Gowda; Samson Mani
Source:
Molecular biology reports 2022 v.49 no.1 pp. 821-826
ISSN:
0301-4851
Subject:
breast neoplasms; breasts; carcinogenesis; cell cycle; cell growth; cell lines; cell movement; cell viability; data collection; disease control; flow cytometry; microRNA; molecular biology; neoplasm cells; quantitative polymerase chain reaction; sequence analysis
Abstract:
... BACKGROUND: The heterogeneity of breast tumors presents a challenge in disease management, necessitating an understanding of the molecular mechanisms driving breast tumorigenesis. Aberrant expression of microRNAs is known to promote tumor growth and progression. Our previous RNA-sequencing dataset revealed the upregulation of miR-362-5p and miR-454-3p in breast tumors. We investigated potential ro ...
DOI:
10.1007/s11033-021-06873-1

22. Alterations in 3D chromatin organization contribute to tumorigenesis of EGFR-amplified glioblastoma

Author:
Qi Yang; Nian Jiang; Han Zou; Xuning Fan; Tao Liu; Xi Huang; Siyi Wanggou; Xuejun Li
Source:
Computational and Structural Biotechnology Journal 2022 v.20 pp. 1967-1978
ISSN:
2001-0370
Subject:
astrocytes; biotechnology; carcinogenesis; cell lines; chromatin; gene expression regulation; genomics; glioblastoma; landscapes; multiomics; mutation; oncogenes; sequence analysis; topology; transcriptome
Abstract:
... EGFR amplification and/or mutation are found in more than half of the cases with glioblastoma. Yet, the role of chromatin interactions and its regulation of gene expression in EGFR-amplified glioblastoma remains unclear. In this study, we explored alterations in 3D chromatin organization of EGFR-amplified glioblastoma and its subsequent impact by performing a comparative analysis of Hi-C, RNA-seq, ...
DOI:
10.1016/j.csbj.2022.04.007

23. Annona cherimola Mill. Leaf Extracts Affect Melanoma Cells Growth and Progression

Author:
Domenico Iacopetta; Alessia Fazio; Chiara La Torre; Alexia Barbarossa; Jessica Ceramella; Fabrizio Francomano; Carmela Saturnino; Hussein El-Kashef; Stefano Alcaro; Maria Stefania Sinicropi
Source:
Foods 2022 v.11 no.16 pp. -
ISSN:
2304-8158
Subject:
Annona cherimola; antineoplastic activity; antioxidants; apoptosis; cell lines; cytoskeleton; dietary supplements; leaves; melanoma; mortality; skin neoplasms; therapeutics
Abstract:
... Cancer represents one of the major causes of mortality worldwide; indeed, 19.3 million new cases and almost 10.0 million deaths were estimated last year. Among the different type of cancers, malignant melanoma represents the most aggressive and deadly skin cancer. Unfortunately, the long-term efficacy of melanoma treatments is limited by the lack of clinical efficacy, onset of side effects and res ...
DOI:
10.3390/foods11162420

24. Anopheles gambiae strain (Ag55) cultured cells originated from Anopheles coluzzii and are phagocytic with hemocyte‐like gene expression

Author:
Ruchir Mishra; Gang Hua; Ujwal R. Bagal; Donald E. Champagne; Michael J. Adang
Source:
Insect science 2022 v.29 no.5 pp. 1346-1360
ISSN:
1672-9609
Subject:
Anopheles gambiae; Escherichia coli; Plasmodium; cell lines; confocal microscopy; flow cytometry; fluorescence microscopy; gene expression; hemocytes; insects; malaria; phagosomes; transcriptome; Africa
Abstract:
... Anopheles gambiae and Anopheles coluzzii are closely related species that are predominant vectors of malaria in Africa. Recently, A. gambiae form M was renamed A. coluzzii and we now conclude on the basis of a diagnostic PCR‐restriction fragment length polymorphism assay that Ag55 cells were derived from A. coluzzii. We established an Ag55 cell transcriptome, and KEGG pathway analysis showed that ...
DOI:
10.1111/1744-7917.13036

25. Anti-Inflammatory, Anti-Diabetic, Anti-Oxidant and Cytotoxicity Assays of South African Herbal Teas and Bush Tea Blends

Author:
Florence Malongane; Lyndy Joy McGaw; Oyinlola Oluwunmi Olaokun; Fhatuwani Nixwell Mudau
Source:
Foods 2022 v.11 no.15 pp. -
ISSN:
2304-8158
Subject:
2,2-diphenyl-1-picrylhydrazyl; Aspalathus linearis; Athrixia; Cercopithecus aethiops; Cyclopia intermedia; anti-inflammatory activity; antioxidant activity; antioxidants; arachidonate 15-lipoxygenase; cell lines; cytotoxicity; glucose; insulin; kidneys; myotubes; South Africa
Abstract:
... South Africa is home to a variety of herbal teas, such as bush tea (Athrixia phylicoides DC.), honeybush tea (Cyclopia intermedia E. Mey and C. subternata Vogel), special tea (Monsonia burkeana Planch. ex Harv.), and rooibos tea (Aspalathus linearis (Burm.f.) R. Dahlgren) that are known to possess anti-oxidant, anti-inflammatory and anti-diabetic properties. The objective of this study was to dete ...
DOI:
10.3390/foods11152233

26. Anti-obesity effects of Ishophloroglucin A from the brown seaweed Ishige okamurae (Yendo) via regulation of leptin signal in ob/ob mice

Author:
Nalae Kang; Seyeon Oh; Seo-Young Kim; Hyosang Ahn; Myeongjoo Son; Soo-Jin Heo; Kyunghee Byun; You-Jin Jeon
Source:
Algal research 2022 v.61 pp. 102533
ISSN:
2211-9264
Subject:
Ishige okamurae; anti-obesity effects; appetite; cell lines; computer simulation; food intake; hypothalamus; immunocytochemistry; insulin; leptin; leptin receptors; macroalgae; models; neurons; obesity; oral administration; phlorotannins; research; secondary metabolites; Korean Peninsula
Abstract:
... Brown seaweeds, which are macroalgae, are common foods and traditional medicines for Asians. Ishige okamurae (Yendo), found in Korea's shallow coastal areas, is one of the edible and medicinal brown seaweeds. Recent studies verified the pharmacological activities of the valuable properties from brown seaweeds such as ishophloroglucin A, a phlorotannin from I. okamurae. Recently, many studies have ...
DOI:
10.1016/j.algal.2021.102533

27. Anti-proliferative and apoptosis-inducing effects of juvenile hormone analogue, fenoxycarb in the Sf21cell line

Author:
C. Ayisha Banu; E.M. Manogem
Source:
Pesticide biochemistry and physiology 2022 v.187 pp. 105182
ISSN:
0048-3575
Subject:
Spodoptera frugiperda; apoptosis; cell lines; cell proliferation; cytotoxicity; fenoxycarb; insecticides; insects; interphase; juvenile hormones; mechanism of action; membrane potential; mitochondrial membrane; pest control
Abstract:
... The apprehension regarding the possible environmental effects of synthetic pesticides has led to the discovery and production of environmental friendly pesticides. Insect hormone mimics, mainly juvenile hormone analogues, like fenoxycarb have acquired attention due to their greater specificity than conventional broad-range insecticides as pest control agents. The study explored the effects of the ...
DOI:
10.1016/j.pestbp.2022.105182

28. Antibacterial, Antioxidant and Melanogenesis Inhibitory Activity of Auraptene, a Coumarin from Ferula szowitsiana Root

Author:
Ensiyeh Charmforoshan; Ehsan Karimi; Ehsan Oskoueian; Mehrdad Iranshahi
Source:
Nutrition and cancer 2022 v.74 no.5 pp. 1829-1836
ISSN:
1532-7914
Subject:
Clostridium perfringens; Escherichia coli; Ferula; Salmonella Typhimurium; Staphylococcus aureus; antibacterial properties; antioxidant activity; catalase; cell lines; coumarin; enzyme inhibition; fibroblasts; functional foods; glutathione peroxidase; humans; melanogenesis; melanoma; mice; nitric oxide; nutrition; superoxide dismutase; thiobarbituric acid; thiocyanates; Central Asia; Iran
Abstract:
... The auraptene is a geranyloxyn coumarin found in the Ferula species. The plant is endemic in Central Asia and it is used as a medicinal food in Iran. This research aimed to evaluate the antibacterial, antioxidant, and anti-melanogenic properties of auraptene, a coumarin from Ferula szowitsiana root. The results revealed that auraptene possessed antibacterial activity with minimum inhibitory and mi ...
DOI:
10.1080/01635581.2021.1962922

29. Antibacterial, antibiofilm and anti‐quorum sensing activities of Muntingia calabura L. leaf extract against Pseudomonas aeruginosa

Author:
K. MosaChristas; E. Kowsalya; R. Karthick; C.R.I. Jaquline
Source:
Letters in applied microbiology 2022 v.75 no.3 pp. 588-597
ISSN:
0266-8254
Subject:
Muntingia calabura; Pseudomonas aeruginosa; agar; antibacterial properties; antioxidant activity; antioxidants; biofilm; cell lines; leaf extracts; methanol; microbiology; microscopy; minimum inhibitory concentration; phytochemicals; toxicity; virulence
Abstract:
... The aim of this study was to evaluate the phytochemicals, antioxidant, antibacterial and anti‐quorum sensing (QS) properties of methanol extract of Muntingia calabura L. leaves against biofilm‐forming strain Pseudomonas aeruginosa. The antioxidant potential of M. calabura methanol leaf extract was evaluated using a radical scavenging assay. Since the findings were so promising, the study aims to s ...
DOI:
10.1111/lam.13595

30. Anticancer Effects of New Ceramides Isolated from the Red Sea Red Algae Hypnea musciformis in a Model of Ehrlich Ascites Carcinoma: LC-HRMS Analysis Profile and Molecular Modeling

Author:
Sameh S. Elhady; Eman S. Habib; Reda F. A. Abdelhameed; Marwa S. Goda; Reem M. Hazem; Eman T. Mehanna; Mohamed A. Helal; Khaled M. Hosny; Reem M. Diri; Hashim A. Hassanean; Amany K. Ibrahim; Enas E. Eltamany; Usama Ramadan Abdelmohsen; Safwat A. Ahmed
Source:
Marine drugs 2022 v.20 no.1 pp. -
ISSN:
1660-3397
Subject:
Hypnea; adenocarcinoma; apoptosis; ascites; blood serum; breasts; caspase-3; cell lines; ceramides; cytotoxicity; doxorubicin; electrospray ionization mass spectrometry; hexadecenoic acid; humans; ketosteroids; liquid chromatography; mice; necrosis; phytochemicals; stearic acid; sterols; terpenoids; vascular endothelial growth factor B; Red Sea
Abstract:
... Different classes of phytochemicals were previously isolated from the Red Sea algae Hypnea musciformis as sterols, ketosteroids, fatty acids, and terpenoids. Herein, we report the isolation of three fatty acids—docosanoic acid 4, hexadecenoic acid 5, and alpha hydroxy octadecanoic acid 6—as well as three ceramides—A (1), B (2), and C (3)—with 9-methyl-sphinga-4,8-dienes and phytosphingosine bases. ...
DOI:
10.3390/md20010063

31. Antimicrobial and immunomodulatory activity of beta-defensin from the Chinese spiny frog (Quasipaa spinosa)

Author:
Shui-Sheng Yu; Zeng-Hui Zhao; Xiao-Fei Gong; Xiao-Li Fan; Zhi-Hua Lin; Jie Chen
Source:
Developmental and comparative immunology 2022 v.126 pp. 104264
ISSN:
0145-305X
Subject:
Aeromonas hydrophila; Streptococcus iniae; Vibrio harveyi; Vibrio vulnificus; antibacterial properties; cell lines; cell membranes; frogs; immunomodulators; innate immunity; macrophages; mice; monocytes; necrosis; neoplasms; transcriptome
Abstract:
... The β-defensins are important components of the vertebrate innate immune system. While mammalian β-defensins have wide-ranging antibacterial and immunomodulatory activities, those of amphibians remain largely uncharacterised. In this study, β-defensin cDNA was identified from the skin transcriptome of the Chinese spiny frog Quasipaa spinosa. This β-defensin (QS-BD) consists of a signal and a matur ...
DOI:
10.1016/j.dci.2021.104264

32. Antineoplastic effects of erufosine on small cell and non-small cell lung cancer cells through induction of apoptosis and cell cycle arrest

Author:
Hüseyin Abdik
Source:
Molecular biology reports 2022 v.49 no.4 pp. 2963-2971
ISSN:
0301-4851
Subject:
apoptosis; caspases; cell cycle checkpoints; cell lines; cell viability; cytotoxicity; dose response; drug therapy; genes; histology; lung neoplasms; molecular biology; plasma membrane; protein synthesis; radiotherapy; surgery; survival rate
Abstract:
... BACKGROUND: Lung cancer (LC) is the most common types of cancer worldwide and is marked by high mortality rate. LC is classified into two major types due to their molecular and histological properties; non-small cell lung cancer (NSCLC) A549 and small cell lung cancer (SCLC). Currently, surgery, chemotherapy and radiation therapy are the most common treatment options of LC. However, the survival r ...
DOI:
10.1007/s11033-022-07117-6

33. Antioxidant Activity of Resveratrol Diastereomeric Forms Assayed in Fluorescent-Engineered Human Keratinocytes

Author:
Ilaria Bononi; Paola Tedeschi; Vanessa Mantovani; Annalisa Maietti; Elisa Mazzoni; Cecilia Pancaldi; Vincenzo Brandolini; Mauro Tognon
Source:
Antioxidants 2022 v.11 no.2 pp. -
ISSN:
2076-3921
Subject:
Scyphozoa; Vitis vinifera; antioxidant activity; antioxidants; cell lines; cell proliferation; cytotoxicity; fluorescence; free radicals; grapes; green fluorescent protein; human nutrition; humans; keratinocytes; models; oxidants; oxidative stress; resveratrol
Abstract:
... Resveratrol is a powerful antioxidant molecule. In the human diet, its most important source is in Vitis vinifera grape peel and leaves. Resveratrol exists in two isoforms, cis- and trans. The diastereomeric forms of many drugs have been reported as affecting their activity. The aim of this study was to set up a cellular model to investigate how far resveratrol could counteract cytotoxicity in an ...
DOI:
10.3390/antiox11020196

34. Antioxidant and anti-proliferative activity of free, conjugates and bound phenolic compounds from tomato and industrial tomato by-product

Author:
Xiomara Patricia Perea-Domínguez; José Alberto Mendoza-Espinoza; Jesús Martin Solano González; José Belisario Leyva-Morales; Laura Gabriela Espinosa-Alonso; Maribel Valdez-Morales
Source:
Journal of food science and technology 2022 v.59 no.6 pp. 2361-2369
ISSN:
0022-1155
Subject:
breast neoplasms; byproducts; cell lines; cytotoxicity; food science; grape tomatoes; grapes; hydroxyl radicals; myoglobin; neoplasm cells; phenolic compounds; superoxide anion; technology
Abstract:
... The aim of this study was to evaluate the antioxidant and anti-proliferative activity of different fractions of phenolic compounds from tomato and tomato by-product. Soluble free phenolics and bound phenolics (BP) fractions from saladette tomato and industrial tomato by-product as well as, conjugated acid-hydrolysable phenolics (AHP) and alkaline-hydrolysable phenolics (AKHP) from saladette tomato ...
DOI:
10.1007/s13197-021-05252-9

35. Antioxidant, antimicrobial and anticarcinogenic activities of bovine milk proteins and their hydrolysates - A review

Author:
Marika Bielecka; Grażyna Cichosz; Hanna Czeczot
Source:
International dairy journal 2022 v.127 pp. 105208
ISSN:
0958-6946
Subject:
anticarcinogenic activity; breast neoplasms; cell lines; health promotion; homeostasis; humans; hydrolysis; immunostimulants; lactoferrin; lung neoplasms; lysozyme; metastasis; milk; neoplasm cells; osteosarcoma; peptides; peroxidase; prostatic neoplasms
Abstract:
... Milk proteins and the products of their hydrolysis are characterised by high levels of biological activity. The health-promoting potential of bovine milk proteins, peptides and amino acids can be attributed to their antioxidant, anti-inflammatory and immunostimulatory properties. Milk proteins, peptides and enzymes play a role in the maintenance of prooxidant/antioxidant homeostasis in the human b ...
DOI:
10.1016/j.idairyj.2021.105208

36. Antiproliferative effects of metformin in cellular models of pheochromocytoma

Author:
Cinthia Gabriel Meireles; Caroline Lourenço de Lima; Marcela Martins de Paula Oliveira; Rafael Abe da Rocha Miranda; Lisa Romano; Teisha Yo-Stella Brashaw; Eliete Neves da Silva Guerra; Francisco de Assis Rocha Neves; J. Paul Chapple; Luiz Alberto Simeoni; Adriana Lofrano-Porto
Source:
Molecular and cellular endocrinology 2022 v.539 pp. 111484
ISSN:
0303-7207
Subject:
adrenal medulla; cell lines; cell proliferation; cell respiration; cell viability; endocrinology; humans; membrane potential; metformin; mitochondrial membrane; neoplasm cells; oxygen consumption; phosphorylation; rats
Abstract:
... Pheochromocytomas (PCCs) are rare neuroendocrine tumors derived from adrenal medulla chromaffin cells. Malignancy and recurrence are rare but demand effective treatment. Metformin exerts antiproliferative effects in several cancer cell lines. We thus evaluated the effects of metformin on cell viability and proliferation, cellular respiration and AMPK-AKT-mTOR-HIFA proliferation pathway on a rat PC ...
DOI:
10.1016/j.mce.2021.111484

37. Antitumor Properties of Epitope-Specific Engineered Vaccine in Murine Model of Melanoma

Author:
Emiliya Stoyanova; Nikolina Mihaylova; Nikola Ralchev; Petya Ganova; Silviya Bradyanova; Iliyan Manoylov; Yuliana Raynova; Krassimira Idakieva; Andrey Tchorbanov
Source:
Marine drugs 2022 v.20 no.6 pp. -
ISSN:
1660-3397
Subject:
Helix aspersa; Rapana; animal models; cancer therapy; cancer vaccines; cell lines; cytotoxicity; flow cytometry; gangliosides; glycoproteins; hemocyanin; immunization; macrophages; melanoma; mice; peptides; snails; spleen
Abstract:
... Finding new effective compounds of natural origin for composing anti-tumor vaccines is one of the main goals of antitumor research. Promising anti-cancer agents are the gastropodan hemocyanins–multimeric copper-containing glycoproteins used so far for therapy of different tumors. The properties of hemocyanins isolated from the marine snail Rapana thomasiana (RtH) and the terrestrial snail Helix as ...
DOI:
10.3390/md20060392

38. Anti‐breast‐cancer activity of self‐fermented Bovistella sinensis Lloyd extracts through the mitochondrial ROS‐induced apoptosis in vitro

Author:
Qinghua Zeng; Ragini Singh; Yong Ye; Shuang Cheng; Feng Kong; Qingmei Zeng
Source:
Journal of food biochemistry 2022 v.46 no.9 pp. e14218
ISSN:
0145-8884
Subject:
anticarcinogenic agents; antineoplastic activity; apoptosis; breast neoplasms; butanol; cell lines; cytotoxicity; ethanol; ethyl acetate; fatty acids; functional foods; ingredients; membrane potential; mitochondria; mitochondrial membrane; neoplasm cells; petroleum; reactive oxygen species
Abstract:
... Bovistella sinensis (BS) Lloyd was medically used by Chinese folks and associated with various bioactivities. In this study, dry fruiting body of Bovistella sinensis (BS) Lloyd was self‐fermented to improve the anti‐breast‐cancer activity and the mitochondrial ROS‐induced apoptosis of key compound was investigated. BS extracts obtained with petroleum ether, ethyl acetate, n‐butanol, ethanol, and d ...
DOI:
10.1111/jfbc.14218

39. Apium graveolens reduced phytofabricated gold nanoparticles and their impacts on the glucose utilization pattern of the isolated rat hemidiaphragm

Author:
Rajasekar Panchamoorthy; Udayamathi Mohan; Anbarasan Muniyan
Source:
Heliyon 2022 v.8 no.1 pp. e08805
ISSN:
2405-8440
Subject:
Apium graveolens; bioactive properties; cell lines; cherries; color; computer simulation; diaphragm; flavonoids; glucose; insulin; nanogold; phytotherapy; polyphenols; rats; zeta potential
Abstract:
... The integration of nanotechnology with herbal medicines overcomes the limitations of herbal therapy and offers desirable bioactivity. Thus, this study was aimed at synthesizing Apium graveolens-gold nanoparticles (AG-AuNPs) and exploring their impact on the glucose utilization pattern of the isolated rat hemidiaphragm. The AG-AuNPs appeared in cherry red color and showed a plasmonic peak at 534nm. ...
DOI:
10.1016/j.heliyon.2022.e08805

40. Apolipoprotein A4 Restricts Diet‐Induced Hepatic Steatosis via SREBF1‐Mediated Lipogenesis and Enhances IRS‐PI3K‐Akt Signaling

Author:
Cheng Cheng; Xiao‐Huan Liu; Jing He; Jing Gao; Jin‐Ting Zhou; Jing‐Na Fan; Xi Jin; Jianbo Zhang; Liao Chang; Zijun Xiong; Jun Yu; Shengbin Li; Xiaoming Li
Source:
Molecular nutrition & food research 2022 v.66 no.18 pp. e2101034
ISSN:
1613-4125
Subject:
adipocytes; beta oxidation; cell lines; fatty liver; food research; gene expression; glucose; hemostasis; hepatocytes; insulin; insulin resistance; insulin secretion; lipogenesis; lipolysis; liver; nutrition; obesity; risk; sequence analysis; therapeutics
Abstract:
... SCOPE: Hepatic steatosis and insulin resistance (IR) are risk factors for many metabolic syndromes such as NAFLD and T2DM. ApoA4 improves glucose hemostasis by increasing glucose‐stimulated insulin secretion and glucose uptake via PI3K‐Akt activation in adipocytes. However, whether ApoA4 has an effect on hepatic steatosis or IR remains unclear. METHODS AND RESULTS: ApoA4‐knockout (KO) aggravates d ...
DOI:
10.1002/mnfr.202101034

41. Apoptotic, antioxidant and cytotoxic properties of synthesized AgNPs using green tea against human testicular embryonic cancer stem cells

Author:
Fahimeh Mobaraki; Mohsen Momeni; Maliheh Jahromi; Farshad Moharrami Kasmaie; Maryam Barghbani; Mohammad Ehsan Taghavizadeh Yazdi; Zahra Meshkat; Fatemeh Homaee Shandiz; Seyed Mousalreza Hosseini
Source:
Process biochemistry 2022 v.119 pp. 106-118
ISSN:
1359-5113
Subject:
Camellia sinensis; acridine orange; antioxidants; apoptosis; biosynthesis; caspase-3; cell lines; cost effectiveness; cytotoxicity; dose response; green tea; humans; inhibitory concentration 50; nanosilver; propidium; testes; ultraviolet-visible spectroscopy
Abstract:
... Silver nanoparticles (AgNPs) are known as carriers of anticancer activities for effectual target treatment of cancer cells. Plant-mediated synthesis for AgNPs synthesis is valuable for biomedical applications due to their more safety and cost-effectiveness. In this work, AgNPs were synthesized successfully using green tea (Camellia sinensis) extract (CSE-AgNPs). The physicochemical properties of C ...
DOI:
10.1016/j.procbio.2022.05.021

42. Arborinine suppresses ovarian cancer development through inhibition of LSD1

Author:
Nan Li; Liang Yang; Hongling Zuo
Source:
Life sciences 2022 v.291 pp. 120275
ISSN:
0024-3205
Subject:
Western blotting; antineoplastic activity; antineoplastic agents; cancer therapy; carcinogenesis; cell lines; cell movement; cell proliferation; cytotoxicity; dose response; drug therapy; epithelium; females; gene expression; mice; neoplasm cells; ovarian neoplasms; paclitaxel; toxicity testing
Abstract:
... Epithelial ovarian carcinoma is the most lethal female reproductive malignancy in the world. Paclitaxel and carboplatin are generally the first-line treatment drugs for ovarian cancer patients, but numerous patients may develop chemotherapy resistance. Thus, it is urgent to identify novel drugs for ovarian cancer treatment. Arborinine has been known as a broad-spectrum anti-tumor agent due to it p ...
DOI:
10.1016/j.lfs.2021.120275

43. Aroma profile and in-silico molecular docking studies of essential oil against “Aspergillosis” from Eucalyptus polybrachtea

Author:
Arun Dev Sharma; Inderjeet Kaur
Source:
South African journal of botany 2022 v.147 pp. 754-763
ISSN:
0254-6299
Subject:
Aspergillus niger; Aspergillus oryzae; Eucalyptus; alternative medicine; antifungal agents; bioactive compounds; cell lines; chitin synthase; cineole; computer simulation; essential oils; fungi; hydrogen; hydrophobicity; ligands; neoplasm cells; odors; oils; toxicity
Abstract:
... Essential oil from Eucalyptus plant is used as traditional and complementary medicine worldwide. It is used as therapeutic agent in food and pharmaceutical companies due to various biological activities. The objective of present study was aromatic profile of essential oil and molecular docking of Eucalyptus essential oil component eucalyptol (1-8 cineole) against Glucosamine-6-phosphate synthase c ...
DOI:
10.1016/j.sajb.2022.03.011

44. Artemisia capillaris Thunb. water extract attenuates adriamycin‐induced renal injury by regulating apoptosis through the ROS/MAPK axis

Author:
Guoshun Huang; Yiye Zhu; Chen Yong; Fang Tian; Li Liu; Qijing Wu; Yang Shu; Min Yao; Chenquan Tang; Xiaofang Wang; Wei Chen; Enchao Zhou
Source:
Journal of food biochemistry 2022 v.46 no.2 pp. e14065
ISSN:
0145-8884
Subject:
Artemisia capillaris; Oriental traditional medicine; acetylcysteine; antioxidants; apoptosis; cell lines; cell viability; doxorubicin; food plants; freeze drying; kidneys; oxidative stress; phosphorylation; renal function; renoprotective effect; signal transduction; superoxide dismutase
Abstract:
... Artemisia capillaris Thunb. is widely used in the treatment of kidney diseases, but the underlying mechanism remain elusive. Therefore, this study aimed to elucidate the mechanism of Artemisia capillaris Thunb. in alleviating renal injury. And renoprotective effects of freeze‐dried powder of Artemisia capillaris Thunb. water extract (WAC) were assessed using adriamycin (ADR)‐induced renal injury t ...
DOI:
10.1111/jfbc.14065

45. Assessment of antioxidant, antimicrobial, and anticancer activities of Sisymbrium officinale plant extract

Author:
Mahmoud Khalid; Mousa Amayreh; Saadi Sanduka; Zaidoun Salah; Fuad Al-Rimawi; Ghassab M. Al-Mazaideh; Abdulkareem A. Alanezi; Fadel Wedian; Fawaz Alasmari; Mohammed Helmy Faris Shalayel
Source:
Heliyon 2022 v.8 no.9 pp. e10477
ISSN:
2405-8440
Subject:
Escherichia coli; Gram-negative bacteria; Sisymbrium officinale; Staphylococcus; Streptococcus; air drying; antimicrobial properties; antineoplastic activity; antioxidant activity; antioxidants; breast neoplasms; breasts; cell lines; colon; ethanol; flavonoids; markets; neoplasm cells; plant extracts; ultrasonic treatment
Abstract:
... The most efficient and safe source of medications is the natural and traditional medications which are produced from plants and herbs. In this study, Sisymbrium officinale (S. officinale) was tested to explore its total phenolic and flavonoids contents. Antioxidant, antimicrobial, and anticancer activities were assessed as well. S. officinale was bought from a local Palestinian market, air-dried, ...
DOI:
10.1016/j.heliyon.2022.e10477

46. Assessment of antioxidant, antiproliferative, anti-inflammatory, and enzyme inhibition activities and UPLC-MS phenolic determination of Ficus carica latex

Author:
Sonia Yahiaoui; Djamel-Edine Kati; Lamiaa M.A. Ali; Khaled El Cheikh; Alain Morére; Chantal Menut; Mostapha Bachir-bey; Nadir Bettache
Source:
Industrial crops and products 2022 v.178 pp. 114629
ISSN:
0926-6690
Subject:
Ficus carica; acetylcholinesterase; antioxidant activity; antioxidants; breast neoplasms; caffeic acid; cell lines; chlorogenic acid; cultivars; cytotoxicity; enzyme inhibition; hepatoma; latex; luteolin; principal component analysis; quercetin; rutin; xanthine oxidase
Abstract:
... The aim of the present investigation consisted in evaluating the antioxidant, cytotoxicity and anti˗inflammatory activities of Ficus carica L. latex of three cultivars (Aberkane “ABR,” Aghanime “AGH”, and EL-bakour “ELB”) and evaluating their in vitro inhibition abilities for several enzymes as well as UPLC˗MS analysis for compound identification. The antioxidant activity was evaluated by ORAC ass ...
DOI:
10.1016/j.indcrop.2022.114629

47. Assessment of fitness of foot‐and‐mouth disease virus A IND 27/2011 as candidate vaccine strain

Author:
Sreenivasa Beeragere Parameshwaraiah; Jajati K. Mohapatra; Veena Jumanal; Dhanesh Valia Valappil; Saravanan Subramaniam; Bhimnere Hanumanthagouda Manjunatha Patel; Suresh H. Basagoudanavar; Madhusudan Hosamani; Brahmadev Pattnaik; Raj Kumar Singh; Aniket Sanyal
Source:
Transboundary and emerging diseases 2022 v.69 no.5 pp. 2996-3000
ISSN:
1865-1674
Subject:
Foot-and-mouth disease virus; amino acids; antigenic variation; capsid; cattle; cell lines; immunogenicity; inactivated vaccines; nucleotide sequences; serotypes; viruses; India
Abstract:
... Antigenic profiling of recent field outbreak strains of foot‐and‐mouth disease virus (FMDV) serotype A in India has revealed considerable antigenic drift from the vaccine strain, A IND 40/2000, necessitating the selection of a new strain. The complete genome sequence of A IND 27/2011 was analysed. Vaccine quality attributes of the new candidate strain including potency as an inactivated vaccine in ...
DOI:
10.1111/tbed.14166

48. The Association between Accumulation of Toxic Advanced Glycation End-Products and Cytotoxic Effect in MC3T3-E1 Cells

Author:
Akiko Sakasai-Sakai; Takanobu Takata; Masayoshi Takeuchi
Source:
Nutrients 2022 v.14 no.5 pp. -
ISSN:
2072-6643
Subject:
advanced glycation end products; bone formation; cell death; cell lines; collagen; cytotoxicity; glucose; glyceraldehyde; metabolism; mice; osteoblasts; osteoporosis; therapeutics; transcription factors
Abstract:
... In diabetic patients, the metabolism of excess glucose increases the toxicity of the aldehyde group of sugar. Aldehydes, including glyceraldehyde (GA), react with intracellular proteins to form advanced glycation end-products (AGEs), which deteriorate bone quality and cause osteoporosis. One of the causes of osteoporotic fractures is impaired osteoblast osteogenesis; however, the cytotoxic effects ...
DOI:
10.3390/nu14050990

49. Association of different biomarkers in vivo and in vitro to assess water quality in Ilha River, Southern Brazil

Author:
Marina Griebeler Moreira; Gabriela Zimmermann Prado Rodrigues; Ana Leticia Hilario Garcia; Diulliane de Jesus Borba; Maria Gabriela Bloedow Scheffer; Laura Cé da Silva; Giovana Piva Peteffi; Rafael Linden; Mariele Feiffer Charão; Günther Gehlen; Magda Susana Perassolo; Ana Luiza Ziulkoski
Source:
International journal of ecohydrology & hydrobiology 2022 pp. -
ISSN:
1642-3593
Subject:
Danio rerio; Daphnia magna; acute toxicity; antioxidant activity; biomarkers; cell lines; chronic toxicity; environmental impact; hydrobiology; laws and regulations; mitochondria; muscles; phosphorus; protein content; rivers; toxicity testing; water quality; Brazil
Abstract:
... The Ilha River is one of the main tributaries of the Sinos River, consequently contributing to its ecological impact. The present study aimed to associate the use of different biomarkers in vitro and in vivo to assess the water quality of three points along the Ilha River during 2019. For this purpose, water samples were collected at the source and mouth of the river, and in an intermediate sectio ...
DOI:
10.1016/j.ecohyd.2022.07.005

50. Astaxanthin decreases the growth-inhibitory dose of cytarabine and inflammatory response in the acute lymphoblastic leukemia cell line NALM-6

Author:
Amirhossein Rastgar; Mahtab Sayadi; Gholamreza Anani-Sarab; Seyed Mehdi Sajjadi
Source:
Molecular biology reports 2022 v.49 no.7 pp. 6415-6422
ISSN:
0301-4851
Subject:
antioxidants; apoptosis; astaxanthin; caspase-8; cell cycle checkpoints; cell lines; cell proliferation; cytotoxicity; drug therapy; drug toxicity; genes; inflammation; inhibitory concentration 50; interleukin-6; lymphocytic leukemia; molecular biology; neoplasm cells; synergism
Abstract:
... BACKGROUND: In spite of the great progress in acute lymphoblastic leukemia (ALL) treatment, a large number of patients still suffer from chemotherapy drug toxicity. As a routine medication for ALL treatment, cytarabine (Ara-C) has many side effects on the patients. Astaxanthin (ASX), on the other hand, is a carotenoid with antioxidant, anti-inflammatory and anti-cancer properties. PURPOSE: The pre ...
DOI:
10.1007/s11033-022-07452-8

51. Autophagy modulation in resveratrol protective effects on steroidogenesis in high-fat diet-fed mice and H2O2-challenged TM3 cells

Author:
Ping Wang; Mengyu Lin; Chao Chen; Zheng-mei Lv
Source:
Molecular biology reports 2022 v.49 no.4 pp. 2973-2983
ISSN:
0301-4851
Subject:
Leydig cells; autophagosomes; autophagy; cell lines; decline; high fat diet; males; mice; mitochondria; molecular biology; oxidative stress; rapamycin; resveratrol; steroidogenesis
Abstract:
... BACKGROUND: Autophagy dysregulation and oxidative stress play critical pathophysiological roles in developing obesity-related metabolic health disorders. This study aims to investigate how autophagy modulation is related to resveratrol (RSV) antioxidant activities and preventive effects on steroidogenesis decline associated with a high-fat diet (HFD) and oxidative damage. METHODS AND RESULTS: Eigh ...
DOI:
10.1007/s11033-022-07120-x

52. BAG2 prevents Tau hyperphosphorylation and increases p62/SQSTM1 in cell models of neurodegeneration

Author:
Raquel S. Lima; Daniel C. Carrettiero; Merari F. R. Ferrari
Source:
Molecular biology reports 2022 v.49 no.8 pp. 7623-7635
ISSN:
0301-4851
Subject:
autophagy; brain stem; cell culture; cell lines; hippocampus; homeostasis; molecular biology; neurodegenerative diseases; neurons; pesticides; phosphorylation; protein value; quality control; rotenone; toxicity
Abstract:
... BACKGROUND: Protein aggregates are pathological hallmarks of many neurodegenerative diseases, however the physiopathological role of these aggregates is not fully understood. Protein quality control has a pivotal role for protein homeostasis and depends on specific chaperones. The co-chaperone BAG2 can target phosphorylated Tau for degradation by an ubiquitin-independent pathway, although its poss ...
DOI:
10.1007/s11033-022-07577-w

53. BDE-209 and TCDD enhance metastatic characteristics of melanoma cells after chronic exposure

Author:
Benisio Ferreira Silva Filho; Francisco Filipak Neto; Micheli de Marchi; Erick Laurent Moggio; Izadora Volpato Rossi; Bruna Sabatke; Marcel Ivan Ramirez; Miguel Clodomiro dos Santos Lucena; Adriane Regina Todeschini; Ciro Alberto de Oliveira Ribeiro
Source:
Environmental pollution 2022 v.313 no. pp. 120140
ISSN:
0269-7491
Subject:
acute exposure; cadherins; carcinogens; cell lines; cell proliferation; cell structures; cell viability; chronic exposure; decabromodiphenyl ether; epithelium; gene expression; humans; melanoma; metalloproteinases; metastasis; mice; phenotype; pollution; secretion; tetrachlorodibenzo-p-dioxin; vimentin
Abstract:
... TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) and BDE-209 (decabromodiphenyl ether) are persistent organic pollutants (POPs) produced by industrial activities and associated with several diseases. TCDD is a known human carcinogen, but few studies investigated about the effects of exposure to both compounds, i.e., whether BDE-209 and TCDD can render tumor cells more aggressive and metastatic. In the c ...
DOI:
10.1016/j.envpol.2022.120140

54. Bacillus subtilis BN strain promotes Th1 response via Toll‐like receptor 2 in polarized mouse M1 macrophage

Author:
Keisuke Tobita; Ryusuke Meguro
Source:
Journal of food biochemistry 2022 v.46 no.2 pp. e14046
ISSN:
0145-8884
Subject:
Bacillus subtilis; Toll-like receptor 2; Toll-like receptor 4; arginase; cell lines; fermentation; functional foods; gene expression; immunostimulants; inducible nitric oxide synthase; interleukin-12; juices; macrophages; mice; natto; soybeans; spores; sporulation; transcription factor NF-kappa B
Abstract:
... Bacillus subtilis BN strain (BN strain) was isolated from natto, a traditional Japanese fermented soybean food product. The present study investigated the Th1 responses of the BN strain on a mouse macrophage cell line, J774.1. In cell cultures, the BN strain (spore cell cultured in Schaeffer’s sporulation media) significantly increased the production of interleukin (IL‐)12 protein. The BN strain i ...
DOI:
10.1111/jfbc.14046

55. Berberine-loaded liquid crystalline nanoparticles inhibit non-small cell lung cancer proliferation and migration in vitro

Author:
Keshav R. Paudel; Meenu Mehta; Geena Hew Suet Yin; Lee Li Yen; Vamshikrishna Malyla; Vyoma K. Patel; Jithendra Panneerselvam; Thiagarajan Madheswaran; Ronan MacLoughlin; Niraj Kumar Jha; Piyush Kumar Gupta; Sachin Kumar Singh; Gaurav Gupta; Pradeep Kumar; Brian G. Oliver; Philip M. Hansbro; Dinesh Kumar Chellappan; Kamal Dua
Source:
Environmental science and pollution research 2022 v.29 no.31 pp. 46830-46847
ISSN:
0944-1344
Subject:
berberine; bioavailability; cell lines; epithelium; humans; liquids; lung neoplasms; lungs; neoplasm cells; neoplasm progression; particle size; pollution; research; tetrazolium; therapeutics; water solubility
Abstract:
... Non-small cell lung cancer (NSCLC) is reported to have a high incidence rate and is one of the most prevalent types of cancer contributing towards 85% of all incidences of lung cancer. Berberine is an isoquinoline alkaloid which offers a broad range of therapeutical and pharmacological actions against cancer. However, extremely low water solubility and poor oral bioavailability have largely restri ...
DOI:
10.1007/s11356-022-19158-2

56. Bevacizumab encapsulation into PLGA nanoparticles functionalized with immunouteroglobin-1 as an innovative delivery system for atherosclerosis

Author:
Giulia De Negri Atanasio; Pier Francesco Ferrari; Ana Baião; Patrizia Perego; Bruno Sarmento; Domenico Palombo; Roberta Campardelli
Source:
International journal of biological macromolecules 2022 pp. -
ISSN:
0141-8130
Subject:
Fourier transform infrared spectroscopy; angiogenesis; atherosclerosis; biocompatibility; blood serum; cell lines; death; drugs; encapsulation; fibronectins; humans; macrophages; mice; monoclonal antibodies; phosphates
Abstract:
... Atherosclerosis represents one of the main causes of death in the Western world. It is a multifactorial pathology characterized by lesions that reduce the lumen of the vessels causing serious clinical events. The extradomain-B of fibronectin is overexpressed during angiogenesis and in tissues undergoing growth and extensive remodeling, i.e., atherosclerotic plaque. Bevacizumab is a recombinant hum ...
DOI:
10.1016/j.ijbiomac.2022.08.063

57. Biflavonoids from the twigs and leaves of Cephalotaxus oliveri Mast. and their α-glucosidase inhibitory activity

Author:
Min Song; Tong Xiao; Qiu-Shuang Wu; Hang Fai Kwok; Ging Chan; Li-Gen Lin; Xiao-Qi Zhang; Wen-Cai Ye; Sheng-Jiang He; Qing-Wen Zhang
Source:
Natural product research 2022 v.36 no.12 pp. 3085-3094
ISSN:
1478-6427
Subject:
Cephalotaxus; cell lines; cytotoxicity; flavonoids; research
Abstract:
... Three new biflavonoids, umcephabiovins C − E (1 − 3), along with fourteen known compounds were isolated from the twigs and leaves of Cephalotaxus oliveri. Their structures and configurations were elucidated by UV, IR, NMR, ECD, and HR-ESI-MS spectra. Compounds 1 − 3 exhibited significant α-glucosidase inhibitory activity with IC₅₀ values of 7.05 ± 2.66, 24.45 ± 4.73, and 1.84 ± 1.14 μM, respective ...
DOI:
10.1080/14786419.2021.1958328

58. Bioactive cytochalasans from the fungus Arthrinium arundinis DJ-13

Author:
Yan Shu; Jia-Peng Wang; Bing-Xian Li; Jun-Li Gan; Hao Ding; Rui Liu; Le Cai; Zhong-Tao Ding
Source:
Phytochemistry 2022 v.194 pp. 113009
ISSN:
0031-9422
Subject:
Apiospora montagnei; Candida albicans; Escherichia coli; antibacterial properties; breast neoplasms; cell lines; cytotoxicity; fungi; kanamycin; metabolites; neoplasm cells; nystatin; plant biochemistry; spectroscopy
Abstract:
... The investigation of the metabolites from Arthrinium arundinis DJ-13 grown in solid medium revealed six undescribed cytochalasans, arundisins A-F and twelve known compounds. Their structures were initially investigated in detail by spectroscopic analyses and were further confirmed by X-crystallography and ECD experiments. In the in vitro bioassays, arundisins A and B showed cytotoxic activity agai ...
DOI:
10.1016/j.phytochem.2021.113009

59. Bioactives of Pomegranate By-Products and Barley Malt Grass Engage in Cereal Composite Bar to Achieve Antimycotic and Anti-Aflatoxigenic Attributes

Author:
Ebtihal Yaqoob Khojah; Ahmed Noah Badr; Dalia Amin Mohamed; Adel Gabr Abdel-Razek
Source:
Foods 2022 v.11 no.1 pp. -
ISSN:
2304-8158
Subject:
aflatoxins; antifungal properties; antioxidants; ascorbic acid; bioactive compounds; biosafety; cell death; cell lines; dough; fast foods; ferulic acid; grasses; inhibitory concentration 50; malting barley; people; pomegranates; sensory evaluation; shelf life
Abstract:
... Food is the source from where a person obtains the body’s daily requirements. People’s current daily habits force them to consume fast food, which is known for its poor nutritional and safety features. So, it is urgent to provide a suitable substitution product to solve this issue. The present investigation aimed to produce a bar with a dual function: nutritional and long shelf life. Two materials ...
DOI:
10.3390/foods11010119

60. Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents

Author:
Marija Ivanov; Jelena Božunović; Uroš Gašić; Danijela Drakulić; Milena Stevanović; Nemanja Rajčević; Dejan Stojković
Source:
Industrial crops and products 2022 v.175 pp. 114260
ISSN:
0926-6690
Subject:
Salvia nemorosa; Staphylococcus lugdunensis; antioxidant activity; antioxidants; biofilm; butanol; cell lines; cell membranes; chemical composition; cytotoxicity; epigallocatechin gallate; ethanol; humans; immunomodulation; keratinocytes; mechanism of action; mitochondria; polyphenols; rosmarinic acid; solvents; therapeutics
Abstract:
... Salvia nemorosa L. is a rich source of bioactive constituents. We have used different solvents to obtain 14 different extracts in order to evaluate solvents impact on S. nemorosa inflorescences bioactivities. Firstly, the extraction yield, chemical composition and cytotoxic properties towards HaCaT cell line were elaborated. Afterwards, we have studied the extracts bioactivity in terms of wound he ...
DOI:
10.1016/j.indcrop.2021.114260

61. Biochemical and biophysical characterization of PADI4 supports its involvement in cancer

Author:
José L. Neira; Salomé Araujo-Abad; Ana Cámara-Artigas; Bruno Rizzuti; Olga Abian; Ana Marcela Giudici; Adrian Velazquez-Campoy; Camino de Juan Romero
Source:
Archives of biochemistry and biophysics 2022 v.717 pp. 109125
ISSN:
0003-9861
Subject:
arginine; biophysics; calcium; carcinogenesis; cell differentiation; cell lines; citrulline; computer simulation; granulocytes; histones; humans; macrophages; molecular dynamics; monocytes; neoplasm cells; pH; protein-arginine deiminase
Abstract:
... PADI4 (protein-arginine deiminase, also known as protein l-arginine iminohydrolase) is one of the human isoforms of a family of Ca²⁺-dependent proteins catalyzing the conversion of arginine to citrulline. Although the consequences of this process, known as citrullination, are not fully understood, all PADIs have been suggested to play essential roles in development and cell differentiation. They h ...
DOI:
10.1016/j.abb.2022.109125

62. Biogenic silver nanoparticle (Bio‐AgNP) has an antibacterial effect against carbapenem‐resistant Acinetobacter baumannii with synergism and additivity when combined with polymyxin B

Author:
Suzane Olachea Allend; Marcelle Oliveira Garcia; Kamila Furtado da Cunha; Déborah Trota Farias de Albernaz; Mirian Elert da Silva; Rodrigo Yudi Ishikame; Luciano Aparecido Panagio; Gerson Nakazaro; Guilherme Fonseca Reis; Daniela Brayer Pereira; Daiane Drawanz Hartwig
Source:
Journal of applied microbiology 2022 v.132 no.2 pp. 1036-1047
ISSN:
1364-5072
Subject:
Acinetobacter baumannii; Fusarium oxysporum; antibacterial properties; bacteria; cell death; cell lines; cell membranes; cytotoxicity; dose response; mammals; minimum inhibitory concentration; nanosilver; polymyxin B; public health; synergism
Abstract:
... AIMS: Carbapenem‐resistant Acinetobacter baumannii represents a public health problem, and the search for new antibacterial drugs has become a priority. Here, we investigate the antibacterial activity of biogenic silver nanoparticles (Bio‐AgNPs) synthesized by Fusarium oxysporum, used alone or in combination with polymyxin B against carbapenem‐resistant A. baumannii. METHODS AND RESULTS: In this s ...
DOI:
10.1111/jam.15297

63. Biological Assay-Guided Fractionation and Mass Spectrometry-Based Metabolite Profiling of Annona muricata L. Cytotoxic Compounds against Lung Cancer A549 Cell Line

Author:
Edcyl Lee O. Salac; Michael Russelle Alvarez; Rnie Shayne Gaurana; Sheryl Joyce B. Grijaldo; Luster Mae Serrano; Florence de Juan; Rowell Abogado; Isagani Padolina Jr.; Froila Marie Deniega; Kimberly Delica; Kimberly Fernandez; Carlito B. Lebrilla; Marlon N. Manalo; Francisco M. Heralde III; Gladys Cherisse J. Completo; Ruel C. Nacario
Source:
Plants 2022 v.11 no.18 pp. -
ISSN:
2223-7747
Subject:
Annona muricata; antineoplastic activity; cell lines; chromatography; cytotoxicity; ethyl acetate; fractionation; lung neoplasms; mass spectrometry; metabolites; metabolomics
Abstract:
... Annona muricata L. (Guyabano) leaves are reported to exhibit anticancer activity against cancer cells. In this study, the ethyl acetate extract from guyabano leaves was purified through column chromatography, and the cytotoxic effects of the semi-purified fractions were evaluated against A549 lung cancer cells using in vitro MTS cytotoxicity and scratch/wound healing assays. Fractions F15-16C and ...
DOI:
10.3390/plants11182380

64. Biological Extraction, HPLC Quantification and Medical Applications of Astaxanthin Extracted from Crawfish “Procambarus clarkii” Exoskeleton By-Product

Author:
Salwa Hamdi; Nour Elsayed; Mohamed Algayar; Verina Ishak; Mariam Ahmed; Sara Ahmed; Mohamed Kamal; Mohamed Abd El-Ghany
Source:
Biology 2022 v.11 no.8 pp. -
ISSN:
2079-7737
Subject:
2,2-diphenyl-1-picrylhydrazyl; Bifidobacterium animalis subsp. lactis; Candida utilis; Lactobacillus delbrueckii subsp. lactis; Saccharomyces cerevisiae; antifungal properties; astaxanthin; breast neoplasms; byproducts; cell lines; cell viability; crayfish; exoskeleton; fungi; hemolysis; hepatoma; high performance liquid chromatography; human health; humans; inhibitory concentration 50; neoplasm cells; probiotics
Abstract:
... The main challenge of astaxanthin extraction is to provide an eco-friendly method of extraction instead of chemical methods that harm human health. This study provided an eco-friendly method for astaxanthin extraction using two bacterial and fungal probiotics (Bifidobacterium lactis, Lactobacillus lactis, Candida utilis, and Saccharomyces cerevisiae, respectively) and determined the astaxanthin co ...
DOI:
10.3390/biology11081215

65. Biological activity of Haematoxylum brasiletto in MCF7 and MDA-MB-231 breast cancer cell lines

Author:
GeorginaVenecia Bello-Martínez; Génesis García-Ramírez; Monserrat Olea-Flores; Napoleón Navarro-Tito; Alberto Hernández-Moreno; Luz Patricia Avila-Caballero; Heriberto Torres-Moreno; Jorge Bello-Martínez
Source:
South African journal of botany 2022 v.146 pp. 528-537
ISSN:
0254-6299
Subject:
Haematoxylum brasiletto; antioxidant activity; antioxidants; breast neoplasms; cell lines; cytotoxicity; epithelial cells; ethyl acetate; flavonoids; heartwood; hexane; methylene chloride; neoplasm cells; traditional medicine; wild plants
Abstract:
... Haematoxylum brasiletto (Fabaceae) is a wild plant from Guerrero, used in traditional medicine. However, there is little scientific information about anti-migratory and antioxidant activities. The aim of this study was to evaluate the anti-migratory and anti-proliferative activities of the ethanolic extract of heartwood of H. brasiletto (HB) and their fractions of hexane (HBA) dichloromethane (HBB ...
DOI:
10.1016/j.sajb.2021.11.017

66. Biological toxicity risk assessment of two potential neutral carbon diesel fuel substitutes

Author:
Silvana Arias; Verónica Estrada; Isabel C. Ortiz; Francisco J. Molina; John R. Agudelo
Source:
Environmental pollution 2022 v.308 pp. 119677
ISSN:
0269-7491
Subject:
DNA; DNA damage; DNA glycosylases; Daphnia pulex; Pulex; butanol; carbon; cell lines; comet assay; cytotoxicity; diesel engines; diesel fuel; ecotoxicology; epithelial cells; genotoxicity; hepatoma; mutagens; oxidation; particulates; pollution; purines; pyrimidines; risk assessment; sulfur; vegetable oil; water solubility
Abstract:
... We investigated the biological response of soluble organic fraction (SOF) and water-soluble fraction (WSF) extracted from particulate matter (PM) emitted by an automotive diesel engine operating in a representative urban driving condition. The engine was fueled with ultra-low sulfur diesel (ULSD), and its binary blends by volume with 13% of butanol (Bu13), and with hydrotreated vegetable oil (HVO) ...
DOI:
10.1016/j.envpol.2022.119677

67. Bioprocess characterization of virus‐like particle production with the insect cell baculovirus expression system at nanoparticle level

Author:
Eduard Puente‐Massaguer; Irene González‐Domínguez; Florian Strobl; Reingard Grabherr; Gerald Striedner; Martí Lecina; Francesc Gòdia
Source:
Journal of chemical technology and biotechnology 2022 v.97 no.9 pp. 2456-2465
ISSN:
0268-2575
Subject:
DNA; bioprocessing; bioreactors; cell lines; drugs; gene therapy; genetic vectors; insects
Abstract:
... Background. Virus‐like particles (VLPs) are a multivalent platform showing great promise for the development of vaccines, gene therapy, diagnostic, and drug delivery approaches. Particularly, HIV‐1 Gag VLPs provide a robust and flexible scaffold for the presentation of a variety of antigens. The insect cell baculovirus expression vector system (BEVS) is nowadays one of the reference systems to pro ...
DOI:
10.1002/jctb.7105

68. Biosafety-inspired structural optimization of triazolium ionic liquids based on structure-toxicity relationships

Author:
Xiujiao Pan; Lingzhi Li; Hsin-Heng Huang; Jialong Wu; Xiaoxia Zhou; Xiliang Yan; Jianbo Jia; Tongtao Yue; Yen-Ho Chu; Bing Yan
Source:
Journal of hazardous materials 2022 v.424 pp. 127521
ISSN:
0304-3894
Subject:
apoptosis; aquatic environment; cations; cell cycle checkpoints; cell lines; cell membranes; cytotoxicity; human health; humans; hydrophobicity; imidazoles; intestines; kidneys; lipidomics; liver; nitrogen; oral exposure; stomach; vapor pressure
Abstract:
... Ionic liquids (ILs), owing to their low vapor pressure and excellent solvating ability, are being increasingly applied in various industries to replace highly toxic organic solvents. They mainly pollute aquatic environment and soils, directly endangering eco-environment and human health. Therefore, it is critical to understand and optimize structural motifs of ILs with reduced toxicity. Considerin ...
DOI:
10.1016/j.jhazmat.2021.127521

69. Bisphenol A interacts with DLGAP5 and regulates IL-6/JAK2/STAT3 signaling pathway to promote tumorigenesis and progression of osteosarcoma

Author:
Yanjun Wang; Yongfeng Yao; Keyvan Ramezani; Moein Bonakdar; Niusha Moghimi; Maryam Salimi; Kai Wang
Source:
Chemosphere 2022 pp. 136545
ISSN:
0045-6535
Subject:
bisphenol A; carcinogenesis; carcinogenicity; carcinogens; cell lines; interleukin-6; metastasis; mice; models; neoplasm cells; neoplasm progression; osteosarcoma; transfection; xenotransplantation
Abstract:
... It has been suggested that Bisphenol A (BPA), a high-production-volume industrial chemical, can accelerate the development of various type of cancers. However, the effect of BPA on osteosarcoma and the underlying mechanisms are yet to be established. Therefore, in this study we tried to explore the carcinogenic effects of BPA on osteosarcoma and the underlying mechanism. SaOs-2 cancer cell line wa ...
DOI:
10.1016/j.chemosphere.2022.136545

70. Block copolymer nanopatterns affect cell spreading: Stem versus cancer bone cells

Author:
R. Fontelo; D. Soares da Costa; R.L. Reis; R. Novoa-Carballal; I. Pashkuleva
Source:
Colloids and surfaces 2022 v.219 pp. 112774
ISSN:
0927-7765
Subject:
adhesion; biocompatible materials; bone fractures; bone marrow; cell lines; composite polymers; computer software; fractal dimensions; humans; molecular weight; morphometry; osteosarcoma; principal component analysis; pyridines; relapse; topography
Abstract:
... Bone healing after a tumor removal can be promoted by biomaterials that enhance the bone regeneration and prevent the tumor relapse. Herein, we obtained several nanopatterns by self-assembly of polystyrene-block-poly-(2-vinylpyridine) (PS-b-P2VP) with different molecular weights and investigated the adhesion and morphology of human bone marrow mesenchymal stem cells (BMMSC) and osteosarcoma cell l ...
DOI:
10.1016/j.colsurfb.2022.112774

71. Boosting effect of testosterone on GDNF expression in Sertoli cell line (TM4); comparison between TM3 cells-produced and exogenous testosterone

Author:
Himasadat Zaker; Mazdak Razi; Alireza Mahmoudian; Farhad Soltanalinejad
Source:
Gene 2022 v.812 pp. 146112
ISSN:
0378-1119
Subject:
Sertoli cells; cell lines; coculture; genes; spermatogenesis; testosterone
Abstract:
... The Glial cell-derived neurotrophic factor (Gdnf) and testosterone induce the spermatogonial stem cells (SSCs) self-renewal and spermatogenesis, respectively. In present study the stimulating role of testosterone on Sertoli cells to produce Gdnf, and the possible effect of Gdnf on Gfrα1 and c-RET expressions were investigated. The TM4 cells (line Sertoli cells) were co-cultured with [0.1, 0.2 and ...
DOI:
10.1016/j.gene.2021.146112

72. Borax Pentahydrate and Disodium Pentaborate Decahydrate Are Candidates as Anti-leukemic Drug Components by Inducing Apoptosis and Changing Bax/Bcl-2 Ratio in HL-60 Cell Line

Author:
Tuğba Erkmen; Belgin Sert Serdar; Halil Ateş; Mehmet Korkmaz; Semra Koçtürk
Source:
Biological trace element research 2022 v.200 no.4 pp. 1608-1616
ISSN:
0163-4984
Subject:
antineoplastic agents; apoptosis; borax; boron; boronic acids; cell lines; cell viability; drug development; flow cytometry; human nutrition; myeloid leukemia; myeloma; propidium; research; therapeutics; toxicity; toxicity testing
Abstract:
... Acute myeloid leukemia (AML) is the most common form of acute leukemia and has the lowest 5-year survival rates. Current treatment strategies do not meet the expectations also. Therefore, there is a need to improve therapeutic approaches still. Boron, which is a natural trace element in human diet, is gaining attention with its important roles in cellular processes for the development of new anti- ...
DOI:
10.1007/s12011-021-02802-2

73. Both full length‐cholesteryl ester transfer protein and exon 9‐deleted cholesteryl ester transfer protein promote triacylglycerol storage in cultured hepatocytes

Author:
Yan Liu; Daniel Mihna; Lahoucine Izem; Richard E. Morton
Source:
Lipids 2022 v.57 no.1 pp. 69-79
ISSN:
0024-4201
Subject:
acetates; adipocytes; adiposity; cell lines; cholesteryl ester transfer protein; exons; fatty acids; fatty liver; hepatocytes; lipogenesis; secretion; triacylglycerols
Abstract:
... We previously reported that overexpression of full‐length cholesteryl ester transfer protein (FL‐CETP), but not its exon 9‐deleted variant (∆E9‐CETP), in an adipose cell line reduces their triacylglycerol (TAG) content. This provided mechanistic insight into several in vivo studies where FL‐CETP levels are inversely correlated with adiposity. However, increased FL‐CETP is also associated with elev ...
DOI:
10.1002/lipd.12330

74. The Bovine Serum Albumin Coated Copper Oxide Nanoparticle for Curcumin Delivery in Biological Environment: In-vitro Drug Release

Author:
Tahmineh Atloo; Ramin Mohammadkhani; Ali Mohammadi; Kasra Arbabi Zaboli; Saeed Kaboli; Hossein Rahimi; Hamed Nosrati; Hosein Danafar
Source:
Journal of polymers and the environment 2022 v.30 no.8 pp. 3203-3208
ISSN:
1566-2543
Subject:
antineoplastic activity; antineoplastic agents; atomic force microscopy; bovine serum albumin; breast neoplasms; cell lines; cupric oxide; curcumin; cytotoxicity; environment; nanocarriers; neoplasm cells; pH; particle size; toxicity testing; transmission electron microscopy
Abstract:
... The copper oxide nanoparticles (CuO NPs) were synthesized by a simple method and then coated with the bovine serum albumin (BSA) to form CuO@BSA NPs. Finally, curcumin (CUR) as an anticancer drug was loaded to the CuO@BSA NPs. The characterization of nanoparticles was carried out by Fourier-transport infrared (FT-IR), ultraviolet-visible (UV-vis), transmission electron microscopy (TEM), and atomic ...
DOI:
10.1007/s10924-022-02401-9

75. Brain targeted delivery of carmustine using chitosan coated nanoparticles via nasal route for glioblastoma treatment

Author:
Saeem Ahmad; Iram Khan; Jayamanti Pandit; Nasr A. Emad; Shahnaj Bano; Khalid Imtiyaz Dar; M. Moshahid Alam Rizvi; Mohd Danish Ansari; Mohd. Aqil; Yasmin Sultana
Source:
International journal of biological macromolecules 2022 v.221 pp. 435-445
ISSN:
0141-8130
Subject:
albino; brain; cell lines; chitosan; drugs; emulsions; evaporation; experimental design; glioblastoma; inhibitory concentration 50; intranasal administration; intravenous injection; nanoparticles; nose; particle size; permeability; solvents
Abstract:
... This study aims to develop chitosan-coated PLGA nanoparticles intended for nose-to-brain delivery of carmustine. Formulations were prepared by the double emulsion solvent evaporation method and optimized by using Box-Behnken Design. The optimized nanoparticles were obtained to satisfactory levels in terms of particle size, PDI, entrapment efficiency, and drug loading. In vitro drug release and ex- ...
DOI:
10.1016/j.ijbiomac.2022.08.210

76. Breast cancer stem cells: mechanobiology reveals highly invasive cancer cell subpopulations

Author:
Martha B. Alvarez-Elizondo; Daphne Weihs
Source:
Cellular and molecular life sciences 2022 v.79 no.3 pp. 134
ISSN:
1420-682X
Subject:
aldehyde dehydrogenase; biophysics; breast neoplasms; cell lines; drug resistance; metastasis; migratory behavior; neoplasm cells; neoplasm progression; risk
Abstract:
... Cancer stem-like cells (CSCs) are a typically small subpopulation of highly tumorigenic cells that can self-renew, differentiate, drive tumor progression, and may mediate drug resistance and metastasis. Metastasis driving CSCs are expected to be highly invasive. To determine the relative invasiveness of CSCs, we isolate distinct subpopulations in the metastatic, MDA-MB-231 breast-cancer cell line, ...
DOI:
10.1007/s00018-022-04181-w

77. CD44-targeted, indocyanine green-paclitaxel-loaded human serum albumin nanoparticles for potential image-guided drug delivery

Author:
Karthik Thangavel; Asha Lakshmikuttyamma; Chellappagounder Thangavel; Sunday A. Shoyele
Source:
Colloids and surfaces 2022 v.209 pp. 112162
ISSN:
0927-7765
Subject:
Western blotting; cell lines; drugs; fibroblasts; fluorescence microscopy; human serum albumin; hyaluronic acid; lung neoplasms; lungs; nanomedicine; paclitaxel
Abstract:
... The use of multifunctional nanomedicines for image-guided drug delivery is currently being universally evaluated as a means of efficiently managing cancers and other diseases. In this study we evaluated the potential of an indocyanine green (ICG) and paclitaxel (PTX) loaded human serum albumin (HSA) nanoparticles that was conjugated with hyaluronic acid for use in image-guided drug delivery target ...
DOI:
10.1016/j.colsurfb.2021.112162

78. Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity

Author:
Sanja Kostić; Aleksandra Vilotić; Andrea Pirković; Dragana Dekanski; Sunčica Borozan; Mirjana Nacka-Aleksić; Svetlana Vrzić-Petronijević; Milica Jovanović Krivokuća
Source:
Food and chemical toxicology 2022 v.163 pp. 112993
ISSN:
0278-6915
Subject:
DNA damage; antioxidant activity; caffeic acid; cell lines; cytotoxicity; diet; direct contact; genotoxicity; humans; lipid peroxidation; mutagens; oxidative stress; pregnancy; toxicology; trophoblast; uterine tissue
Abstract:
... Caffeic acid is highlighted as one of the major phenolic compounds present in foods with known antioxidant activity. This phenolic is among commonly consumed substances in everyday diet of pregnant women. However, there is not enough information on its effects during pregnancy, especially the most vulnerable early stage. Extravillous trophoblast cells are specific cells of the placenta that come i ...
DOI:
10.1016/j.fct.2022.112993

79. Calcium and integrin-binding protein 1-like interacting with an integrin α-cytoplasmic domain facilitates cellular immunity in Helicoverpa armigera

Author:
Ming-Ming Zhang; Ling-Ling Luo; Yu Liu; Gui-Jie Wang; Huan-Huan Zheng; Xu-Sheng Liu; Jia-Lin Wang
Source:
Developmental and comparative immunology 2022 v.131 pp. 104379
ISSN:
0145-305X
Subject:
Helicoverpa armigera; calcium; calorimetry; cell adhesion; cell lines; cell-mediated immunity; ecdysterone; encapsulation; genes; hemocytes; insects; integrins; phagocytosis; titration; transcriptomics
Abstract:
... Integrins are transmembrane receptor heterodimers composed of α and β subunits. They are known to mediate extracellular signals to promote cell adhesion and spreading, and are therefore essential for cellular immunity. However, proteins that bind to integrin cytoplasmic domains and mediate intracellular signaling to promote cell adhesion require identification. Calcium and integrin-binding protein ...
DOI:
10.1016/j.dci.2022.104379

80. Canine Epidermal Keratinocytes (CPEK) Grown in Monolayer Are Not Representative of Normal Canine Keratinocytes for Permeability Studies: Pilot Studies

Author:
Rosanna Marsella; Rachel Wilkes; Kim Ahrens
Source:
Veterinary sciences 2022 v.9 no.1 pp. -
ISSN:
2306-7381
Subject:
antibodies; cell lines; dogs; electrical resistance; epithelium; keratinocytes; permeability; tight junctions
Abstract:
... Canine progenitor epidermal keratinocytes (CPEK) are used as canine keratinocyte cell line. Their suitability for skin barrier studies is unknown. Measurement of transepithelial electric resistance (TEER) evaluates epithelial permeability. We compared TEER and tight junction (TJ) expression in CPEKs and normal keratinocytes (NK) harvested from biopsies of normal dogs. CPEKs and NK were grown until ...
DOI:
10.3390/vetsci9010025

81. Carboxymethyl starch encapsulated 5-FU and DOX co-loaded layered double hydroxide for evaluation of its in vitro performance as a drug delivery agent

Author:
Elaheh Ranjbar; Hassan Namazi; Malihe Pooresmaeil
Source:
International journal of biological macromolecules 2022 v.201 pp. 193-202
ISSN:
0141-8130
Subject:
X-ray diffraction; cancer therapy; cell lines; cell viability; colorectal neoplasms; doxorubicin; drug delivery systems; fluorouracil; microparticles; neoplasm cells; starch; viability assays
Abstract:
... Achieving a new oral drug delivery system with controlled drug release behavior is valuable in cancer therapy. Therefore, for the first time, doxorubicin (DOX) and 5-fluorouracil (5-Fu) were simultaneously co-loaded on the as-synthesized layered double hydroxides LDH(MgAl). The resulted system was encapsulated with carboxymethyl starch to improve its efficiency for colon cancer therapy. Several ch ...
DOI:
10.1016/j.ijbiomac.2021.12.181

82. Carotane sesquiterpenoids A–G from the desert endophytic fungus Fusarium sp. HM 166

Author:
Bing-Wen Zhang; Li Jiang; Zhuang Li; Xue-Hui Gao; Fei Cao; Xin-hua Lu; Wen-Bin Shen; Xue-Xia Zhang; Fan-Dong Kong; Du-Qiang Luo
Source:
RSC advances 2022 v.12 no.38 pp. 24590-24595
ISSN:
2046-2069
Subject:
Fusarium; X-ray diffraction; breasts; cell lines; culture media; cytotoxicity; endophytes; fungi; hepatoma; humans; mutants; neoplasm cells; sesquiterpenoids; spectral analysis
Abstract:
... Seven undescribed carotane sesquiterpenoids named fusanoids A–G (1–7), along with one known analog (8) and two known sesterterpenes (9 and 10), were isolated from the fermentation broth of the desert endophytic fungi Fusarium sp. HM166. The structures of the compounds, including their absolute configurations, were determined by spectroscopic data, single-crystal X-ray diffraction analysis, and ECD ...
DOI:
10.1039/d2ra02762c

83. Caryocar brasiliense Camb. fruit peel butanolic fraction induces antiproliferative effects against murine melanoma cell line

Author:
Jéssica Nayara Basílio Silva; Victor Hugo Dantas Guimarães; Barbhara Mota Marinho; Amanda Souto Machado; Amanda Rodrigues Santos; Ludmilla Regina de Souza David; Geraldo Aclécio Melo; Alfredo Maurício Batista de Paula; Sérgio Henrique Sousa Santos
Source:
Phytomedicine plus 2022 v.2 no.2 pp. 100273
ISSN:
2667-0313
Subject:
Caryocar brasiliense; acridine orange; cancer therapy; cell death; cell lines; fruit peels; gallic acid; gas chromatography; inhibitory concentration 50; melanoma; mice; migratory behavior; skin neoplasms; traditional medicine
Abstract:
... Cutaneous malignant melanoma is a skin cancer type highly resistant to standard cancer therapies. Natural compounds have been reported as important sources for the creation of new drugs for cancer treatment. Caryocar brasiliense Camb., popularly known as Pequi, is often used in Brazilian folk medicine, with anticancer effects reported. Objectives: The present study evaluated the antiproliferative ...
DOI:
10.1016/j.phyplu.2022.100273

84. Catechin Metabolites along with Curcumin Inhibit Proliferation and Induce Apoptosis in Cervical Cancer Cells by Regulating VEGF Expression In-Vitro

Author:
Elnaz Khojaste; Changiz Ahmadizadeh
Source:
Nutrition and cancer 2022 v.74 no.3 pp. 1048-1057
ISSN:
1532-7914
Subject:
Western blotting; apoptosis; carcinogenesis; catechin; cell lines; cell proliferation; cell viability; curcumin; cytotoxicity; death; flow cytometry; green tea; intestinal microorganisms; metabolites; microRNA; neoplasm cells; nutrition; quantitative polymerase chain reaction; therapeutics; toxicity testing; uterine cervical neoplasms
Abstract:
... Cervical cancer is the fourth most common cancer and the second cause of cancer-related death among women. Over the past two decades, green tea catechins and curcumin have received much attention for their role in preventing carcinogenesis. In this study, we evaluated the effects of the catechin metabolites and curcumin on cervical cancer cell proliferation and apoptosis. For this aim, the Ca Ski ...
DOI:
10.1080/01635581.2021.1936082

85. Cautions should be taken when using cell models for gastric cancer research

Author:
Siqi Cai; Dan Yao; Yuqi Zhang; Zhaohe Li; Xiaoyu Li; Li Li
Source:
Gene 2022 v.806 pp. 145922
ISSN:
0378-1119
Subject:
DNA methylation; biomarkers; cell lines; cohort studies; computer simulation; data collection; drugs; gene expression regulation; genes; neoplasm cells; stomach neoplasms
Abstract:
... Gastric cancer (GC)-derived cell lines were generally used in basic cancer research and drug screening. However, it is always concerned about the difference between cultured cells and primary tumor by oncologists. To address this question, we compared differentially expressed genes (DEGs) in primary cancers, healthy tissues, and cell lines both in vitro and in silico. Seven reported genes with dec ...
DOI:
10.1016/j.gene.2021.145922

86. Cell-line and culture model specific responses to organic contaminants in house dust: Cell bioenergetics, oxidative stress, and inflammation endpoints

Author:
Mauricius Marques dos Santos; Megan Tan Pei Fei; Caixia Li; Shenglan Jia; Shane Allen Snyder
Source:
Environment international 2022 v.167 pp. 107403
ISSN:
0160-4120
Subject:
batters; biochemical pathways; biomarkers; cell lines; cell respiration; cytokines; dose response; dust; energy metabolism; environment; epithelium; hepatocytes; inflammation; liquid-air interface; lungs; models; monocytes; oxidative stress; reactive oxygen species; risk assessment
Abstract:
... Exposure to organic contaminants in house dust is linked to the development or exacerbation of many allergic and immune disorders. In this work, we evaluate the effects of organic contaminants on different cell bioenergetics endpoints using five different cell lines (16HBE14o-, NuLi-1, A549, THP-1 and HepG2), and examine its effects on lung epithelial cells using conventional 2D and 3D (air–liquid ...
DOI:
10.1016/j.envint.2022.107403

87. Cell-line screening and process development for a fusogenic oncolytic virus in small-scale suspension cultures

Author:
Sven Göbel; Fabian Kortum; Karim Jaén Chavez; Ingo Jordan; Volker Sandig; Udo Reichl; Jennifer Altomonte; Yvonne Genzel
Source:
Applied microbiology and biotechnology 2022 v.106 no.13-16 pp. 4945-4961
ISSN:
0175-7598
Subject:
Avian orthoavulavirus 1; Vesiculovirus; bioreactors; cell death; cell lines; genetically modified organisms; giant cells; hybrids; immunotherapy; manufacturing; metabolism; neoplasms; viruses
Abstract:
... Oncolytic viruses (OVs) represent a novel class of immunotherapeutics under development for the treatment of cancers. OVs that express a cognate or transgenic fusion protein is particularly promising as their enhanced intratumoral spread via syncytia formation can be a potent mechanism for tumor lysis and induction of antitumor immune responses. Rapid and efficient fusion of infected cells results ...
DOI:
10.1007/s00253-022-12027-5

88. Cell-to-cell trafficking patterns in cell lines of Araucaria angustifolia (Brazilian pine) with contrasting embryogenic potential

Author:
Bruno V. Navarro; Paula Elbl; Leandro F. de Oliveira; Amanda R. Piovezani; André L. W. dos Santos; Diego T. de Souza; Diego Demarco; Marcos S. Buckeridge; Eny I. S. Floh
Source:
Plant cell, tissue, and organ culture 2022 v.148 no.1 pp. 81-93
ISSN:
0167-6857
Subject:
Araucaria angustifolia; callose; cell communication; cell lines; embryogenesis; gene expression; gene expression regulation; hydrolases; immunocytochemistry; organ culture; plasmodesmata; proteome; transcriptome; transmission electron microscopy
Abstract:
... The acquisition of embryogenic competence involves aspects at a cellular level related to cell communication and signaling. For Araucaria angustifolia, transcriptome and proteome databases have indicated differential gene expression and protein abundance related to cell-to-cell communication during embryogenesis. The differences are mainly related to vesicular transport and regulation of plasmodes ...
DOI:
10.1007/s11240-021-02166-4

89. A Census of Hsp70-Mediated Proteome Solubility Changes upon Recovery from Heat Stress

Author:
Xiaojing Sui; Dezerae Cox; Shuai Nie; Gavin E. Reid; Danny M. Hatters
Source:
Journal of proteome research 2022 v.21 no.5 pp. 1251-1261
ISSN:
1535-3907
Subject:
cell lines; energy metabolism; heat shock response; heat stress; heat-shock protein 70; mice; microfilaments; protein value; proteome; quality control; research; solubility
Abstract:
... Eukaryotic cells respond to heat shock through several regulatory processes including upregulation of stress responsive chaperones and reversible shutdown of cellular activities through formation of protein assemblies. However, the underlying regulatory mechanisms of the recovery of these heat-induced protein assemblies remain largely elusive. Here, we measured the proteome abundance and solubilit ...
DOI:
10.1021/acs.jproteome.1c00920

90. Characterization and Functional Study of FAM49B Reveals Its Effect on Cell Proliferation in HEK293T Cells

Author:
Yijian Chen; Yuyan Jiang; Jihui Lao; Yankuan Zhou; Lida Su; Xiao Huang
Source:
Genes 2022 v.13 no.2 pp. -
ISSN:
2073-4425
Subject:
CRISPR-Cas systems; cell lines; cell proliferation; cyclins; cytoskeleton; gene expression; sequence homology
Abstract:
... FAM49B/Fam49b is a member of the Fam49 (Family with sequence similarity 49) gene family, which is characterized by the conserved domain, DUF1394 (Domain of Unknown Function 1394). It has also been named CYRI-B (CYFIP related RAC1 interactor B), implicating its important function of regulating RAC1-driven cytoskeleton remolding. In this study, to further investigate its functions and mechanisms aff ...
DOI:
10.3390/genes13020388

91. Characterization of Some Microorganisms from Human Stool Samples and Determination of Their Effects on CT26 Colorectal Carcinoma Cell Line

Author:
Münevver Kahraman; Aynur Gül Karahan; Mustafa Ender Terzioğlu
Source:
Current microbiology 2022 v.79 no.8 pp. 225
ISSN:
0343-8651
Subject:
Candida kefyr; Cryptococcus neoformans; amphotericin B; ampicillin; antioxidant activity; bile; cell lines; chloramphenicol; colorectal neoplasms; digestive tract; erythromycin; exopolysaccharides; feces; fluconazole; gentamicin; itraconazole; kanamycin; ketoconazole; lactic acid; mucosa; neoplasm cells; nystatin; probiotics; proteolysis; streptomycin; tetracycline; vancomycin; viability; yeasts
Abstract:
... The present study aimed to isolate and identify the potential probiotic, pathobiont, and pathogenic microorganisms in the stool samples of 12 healthy individuals and evaluate their in vitro effects on cancer formation. A total of 83 strains were isolated from the stool samples and identified using MALDI-Biotyper. Fourteen of the isolates were identified as Candida spp., three isolates were identif ...
DOI:
10.1007/s00284-022-02915-4

92. Characterization of a novel picornavirus isolated from moribund gilthead seabream (Sparus aurata) larvae

Author:
Lénaïg Louboutin; Nolwenn M Dheilly; Joëlle Cabon; Sara Picon Camacho; Aurélie Leroux; Pierrick Lucas; Alain Le Breton; Yannick Blanchard; Thierry Morin
Source:
Journal of fish diseases 2022 v.45 no.5 pp. 707-716
ISSN:
0140-7775
Subject:
Sparus aurata; anorexia; aquaculture; cell culture; cell lines; eel; etiology; hatcheries; intestinal mucosa; mortality; polyproteins; quantitative polymerase chain reaction; viral genome; viruses
Abstract:
... Gilthead seabream represents a species of importance in Mediterranean aquaculture. The larval stage is particularly sensitive and frequently impacted in suboptimal environmental or sanitary conditions. In the present study, investigations were carried out in a seabream hatchery following an unusual mortality reaching 70% among 50‐day post‐hatching. Anorexia, loss of appetite and abnormal swimming ...
DOI:
10.1111/jfd.13596

93. Characterization of grass carp FosB, Fosl2, JunD transcription factors in response to GCRV infection

Author:
Fei Yu; Wanjuan Li; Longlong Wang; Shunzheng Que; Liqun Lu
Source:
Aquaculture and fisheries 2022 v.7 no.3 pp. 304-312
ISSN:
2468-550X
Subject:
Ctenopharyngodon idella; RNA; Reoviridae; aquaculture; cell lines; fish; inflammation; kidneys; phylogeny; sequence analysis; tissue distribution
Abstract:
... Activating protein-1 (AP-1) composed of Jun and Fos family proteins, which are frequently activated by pathogens to induce cellular inflammation and immune responses. In this study, by using RNA sequencing (RNA-seq) technology, we found that FosB, Fosl2, and JunD expression was co-induced 12 and 20 h in grass carp (Ctenopharyngodon idella) kidney cell line (CIK cells) after grass carp reovirus (GC ...
DOI:
10.1016/j.aaf.2020.08.002

94. Characterizing genes associated with cancer using the CRISPR/Cas9 system: A systematic review of genes and methodological approaches

Author:
Fernando Gonzalez-Salinas; Claudia Martinez-Amador; Victor Trevino
Source:
Gene 2022 v.833 pp. 146595
ISSN:
0378-1119
Subject:
CRISPR-Cas systems; RNA; carcinogenesis; cell lines; genes; mutation; systematic review; transfection
Abstract:
... The CRISPR/Cas9 system enables a versatile set of genomes editing and genetic-based disease modeling tools due to its high specificity, efficiency, and accessible design and implementation. In cancer, the CRISPR/Cas9 system has been used to characterize genes and explore different mechanisms implicated in tumorigenesis. Different experimental strategies have been proposed in recent years, showing ...
DOI:
10.1016/j.gene.2022.146595

95. Chemical characterization of non‐psychoactive Cannabis sativa L. extracts, in vitro antiproliferative activity and induction of apoptosis in chronic myelogenous leukaemia cancer cells

Author:
Lisa Anceschi; Alessandro Codeluppi; Virginia Brighenti; Riccardo Tassinari; Valentina Taglioli; Lucia Marchetti; Luca Roncati; Andrea Alessandrini; Lorenzo Corsi; Federica Pellati
Source:
Phytotherapy research 2022 v.36 no.2 pp. 914-927
ISSN:
0951-418X
Subject:
Cannabis sativa; apoptosis; cannabidiol; caspase-3; cell cycle; cell lines; cell proliferation; cytosol; dose response; flow cytometry; hemp; humans; leukemia; mass spectrometry; membrane potential; mitochondrial membrane; phytotherapy; research; ultra-performance liquid chromatography
Abstract:
... In this study, extracts from non‐psychoactive Cannabis sativa L. varieties were characterized by means of ultra high‐performance liquid chromatography coupled with high‐resolution mass spectrometry (UHPLC‐HRMS) and their antiproliferative activity was assessed in vitro. The human chronic myelogenous leukaemia cell line K562 was chosen to investigate the mechanism of cell death. The effect on the c ...
DOI:
10.1002/ptr.7357

96. Chemical constituents and cytotoxic activity of Ocimum gratissimum L.

Author:
Dieu Ne Dort Nganteng; Raduis Melong; Estelle Phylicia Mbiekop; Timoleon Maffo; Éric Allémann; Florence Delie; Pascal Wafo; Bonaventure Ngadjui Tchaleu; Jean Paul Dzoyem
Source:
South African journal of botany 2022 v.150 pp. 330-333
ISSN:
0254-6299
Subject:
Ocimum gratissimum; adenocarcinoma; breast neoplasms; cell lines; cytotoxicity; drug therapy; drugs; fibroblasts; flavonoids; leukemia; lungs; macrophages; metabolites; mice; neoplasm cells; paclitaxel; triterpenoids
Abstract:
... Malignancies constitute a global health concern and chemotherapy remains the main mode of treatment. The present study was designed to evaluate the cytotoxicity of metabolites isolated from Ocimum gratissimum L. The phytochemical investigation of the leaves of O. gratissimum L. (Lamiaceae) led to the isolation and characterization of eight compounds. Structures of isolated compounds were elucidate ...
DOI:
10.1016/j.sajb.2022.07.029

97. Chemical constituents and their antioxidant and anti-inflammatory activities from edible Cornus officinalis fruits

Author:
Zhongcan Peng; Yuming Wang; Jun He; Jia Zhang; Xuege Pan; Xiansheng Ye; Weiku Zhang; Jiekun Xu
Source:
European food research & technology 2022 v.248 no.4 pp. 1003-1010
ISSN:
1438-2377
Subject:
2,2-diphenyl-1-picrylhydrazyl; Cornus officinalis; acid hydrolysis; cell lines; electrospray ionization mass spectrometry; food production; food research; functional foods; inflammation; interleukin-6; lignans; lipopolysaccharides; macrophages; nitric oxide; phytochemicals; technology; traditional medicine
Abstract:
... Cornus officinalis fruits, an edible functional food with the tonic effect, belongs to the Cornaceae family. It is widely used in both traditional medicine and in some food production. A phytochemical study on the fruits of Cornus officinalis resulted in 20 structurally diverse compounds including 2 new lignans, named officinalignans A-B (1–2), and 2 new iridoids, named loganin-(4′-O-7″)-α-morroni ...
DOI:
10.1007/s00217-021-03940-6

98. Chemical constituents from the Streptomyces morookaensis strain Sm4-1986

Author:
Gui-Yun Wu; Zhi-Yan Zhu; Xiao Zhang; Miao-Miao Wang; Jian-Xiong Li; Ying-Jie Hu; Hai-Bo Tan
Source:
Natural product research 2022 v.36 no.14 pp. 3681-3688
ISSN:
1478-6427
Subject:
Bacillus cereus; Streptomyces morookaensis; cell lines; methicillin-resistant Staphylococcus aureus; neoplasm cells; research
Abstract:
... Three new compounds, including 6-methoxy-3,4,5,7-tetramethylisochromane-3,8-diol (1), 3,4,5,7-tetramethylisochromane-3,6,8-triol (2), streptimidone derivative (3), along with ten known compounds (4-13) were isolated from the Streptomyces morookaensis strain Sm4-1986. Their chemical structures were established based on the information from UV, IR, NMR (¹H NMR, ¹³C NMR, ¹H-¹H COSY, HSQC, HMBC, NOESY ...
DOI:
10.1080/14786419.2021.1881095

99. Chemical inhibition of ER aminopeptidase 1 as a tool for regulating the immunopeptidome of cancer cells

Author:
Despoina Koumantou; Eilon Barnea; Adrian Martin-Esteban; Zachary Maben; Athanasios Papakyriakou; Paraskevi Kokkala; Harris Pratsinis; Dimitris Georgiadis; Lawrence J. Stern; Arie Admon; Efstratios Stratikos
Source:
Molecular immunology 2022 v.150 no. pp. -
ISSN:
0161-5890
Subject:
T-lymphocytes; aminopeptidases; antigen presentation; cell lines; chromatography; cytotoxicity; epitopes; immunogenicity; immunotherapy; intracellular enzymes; ligands; melanoma; peptides; tandem mass spectrometry
Abstract:
... The efficacy of cancer immunotherapy, including treatment with immune-checkpoint inhibitors, is often limited by ineffective presentation of antigenic peptides that can elicit T-cell mediated anti-tumor cytotoxic responses. Therefore, manipulating antigen presentation is an emerging approach for enhancing the immunogenicity of tumors in immunotherapy settings. ER aminopeptidase 1 (ERAP1) is an int ...
DOI:
10.1016/j.molimm.2022.05.050

100. Chemically Tuning Resveratrol for the Effective Killing of Gram-Positive Pathogens

Author:
Rubén Cebrián; Qian Li; Pablo Peñalver; Efres Belmonte-Reche; María Andrés-Bilbao; Ricardo Lucas; María Violante de Paz; Oscar P. Kuipers; Juan Carlos Morales
Source:
Journal of natural products 2022 v.85 no.6 pp. 1459-1473
ISSN:
1520-6025
Subject:
aminoglycosides; antibacterial properties; antibiotic resistance; antimicrobial peptides; cell lines; cross resistance; hemolysis; mechanism of action; membrane permeability; resveratrol; synergism; toxicity
Abstract:
... In the era of antimicrobial resistance, the identification of new compounds with strong antimicrobial activity and the development of alternative therapies to fight drug-resistant bacteria are urgently needed. Here, we have used resveratrol, a safe and well-known plant-derived stilbene with poor antimicrobial properties, as a scaffold to design several new families of antimicrobials by adding diff ...
DOI:
10.1021/acs.jnatprod.1c01107