U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Subject term cell lines Remove constraint Subject term: cell lines
Start Over

Search

14,614  Search Results

« Previous | 1 - 20 of 14,614 |
Number of results to display per page

Search Results

1. Characterization of pIgR and its association with resistance to iridovirus in Asian seabass

Author:
Yi Xuan Tay; Yepin Yu; Zituo Yang; Le Wang; Fei Sun; Gen Hua Yue
Source:
Aquaculture 2023 v.562 pp. 738783
ISSN:
0044-8486
Subject:
Lates calcarifer; Singapore grouper iridovirus; aquaculture; cell lines; coat proteins; disease resistance; eyes; fingerlings; genes; genotyping; heart; immunoglobulins; intestines; kidneys; mucosal immunity; polymers
Abstract:
... The improvement of disease resistance is vital in aquaculture as diseases are one of the serious threats to successful aquaculture. Understanding of molecular mechanisms underlying disease resistance is important in improving resistance against diseases. The pIgR (polymeric immunoglobulin receptor) gene plays an important role in the mucosal immunity of fish. However, not much is known about its r ...
DOI:
10.1016/j.aquaculture.2022.738783

2. Diagnostic kit based on halloysite nanoclay-ionic liquid nanocomposite modified electrode for electrochemical determination of cancer biomarker

Author:
Esma Yildiz; Beste Yurdacan; Yasemin Erac; Arzum Erdem
Source:
Talanta 2023 v.252 pp. 123854
ISSN:
0039-9140
Subject:
DNA probes; RNA; analytical kits; biomarkers; breast neoplasms; carbon electrodes; cell lines; detection limit; dielectric spectroscopy; electrochemistry; energy-dispersive X-ray analysis; halloysite; humans; hybridization; inosine; kidneys; liquids; nanocomposites; neoplasm cells; nucleic acid hybridization; nucleotide sequences; voltammetry
Abstract:
... Nucleic acid hybridization is occurred between the selective single-stranded nucleic acid sequence and its target sequence, which is one of the essential procedure for electrochemical detection of nucleic acid. microRNA-21 (miRNA-21) is known as a biomarker in various cancers. The determination of miRNA-21 was attained through by hybridization of inosine substituted miRNA-21 specific DNA probe (Pᵢ ...
DOI:
10.1016/j.talanta.2022.123854

3. Establishment and characterization of a cell line from the brain of the Japanese flounder (Paralichthys olivaceus) and its application in the study of viral infection

Author:
Zhaodi Sun; Yuqin Ren; Yitong Zhang; Yucong Yang; Guixing Wang; Zhongwei He; Yufeng Liu; Wei Cao; Yufen Wang; Yuanshuai Fu; Jilun Hou
Source:
Aquaculture 2023 v.562 pp. 738825
ISSN:
0044-8486
Subject:
Andrias davidianus; Hirame rhabdovirus; Iridovirus; Paralichthys olivaceus; actin; aquaculture; brain; cell lines; chromosome number; chromosomes; diploidy; fish; gene expression; genes; host-pathogen relationships; interleukin-8; karyotyping; neural stem cells; temperature; transfection; viruses
Abstract:
... Fish cell lines are important tools in many biological studies. Here, we established a new Japanese flounder brain (JFB) cell line from the brain tissue of the Japanese flounder (Paralichthys olivaceus), which was successfully sub-cultured for over 120 passages, and consisted predominantly of fibroblast-like cells. Sequencing of the actin gene confirmed that JFB cells were derived from the Japanes ...
DOI:
10.1016/j.aquaculture.2022.738825

4. Establishment of a new intestinal cell line from the grouper (Epinephelus coioides) for aquatic virus pathogenesis

Author:
Jieliang Fan; Jiaying Zheng; Wenjie Lai; Shijia Liu; Xia Liang; Quan Wang; Youhua Huang; Jingguang Wei; Xiaohong Huang; Qiwei Qin; Sheng Zhou
Source:
Aquaculture 2023 v.562 pp. 738771
ISSN:
0044-8486
Subject:
Epinephelus coioides; Redspotted grouper nervous necrosis virus; Singapore grouper iridovirus; aquaculture; cell lines; chromosome number; cryopreservation; cytogenetic analysis; cytokines; cytopathogenicity; electron microscopy; fetal bovine serum; fluorescence; grouper; host-pathogen relationships; interferons; intestines; marine fish; pathogenesis; ribosomal RNA; virus replication; viruses
Abstract:
... A new cell line (ECGI-21) was developed from the intestine of grouper (Epinephelus coioides). The cell line was routinely grown in Leibovitz's L-15 medium supplemented with 15% fetal bovine serum (FBS) at 28 °C. ECGI-21 cells had an epithelioid morphology. At present, the cell line has been stably passed over 80 generations and has been successfully cryopreserved. The origin of grouper was confirm ...
DOI:
10.1016/j.aquaculture.2022.738771

5. Fate and PPARγ and STATs-driven effects of the mitochondrial complex I inhibitor tebufenpyrad in liver cells revealed with multi-omics

Author:
Thibaut Léger; Patrick Balaguer; Ludovic Le Hégarat; Valérie Fessard
Source:
Journal of hazardous materials 2023 v.442 pp. 130083
ISSN:
0304-3894
Subject:
beta oxidation; cell culture; cell lines; dose response; hepatocytes; humans; inflammation; interleukin-6; liver; metabolites; metabolomics; mitochondria; multiomics; oxidative phosphorylation; pesticides; proteasome endopeptidase complex; proteomics; rats; secretion; transcription factors
Abstract:
... The biological effects of the pesticide and mitochondrial complex I inhibitor tebufenpyrad (TEBU) on liver cells were investigated by combining proteomics and metabolomics. Both cell culture media and cellular lysates were analyzed in dose-response and kinetic experiments on the HepaRG cell line. Responses were compared with those obtained on primary human and rat hepatocytes. A multitude of phase ...
DOI:
10.1016/j.jhazmat.2022.130083

6. Food peptides as inducers of CCK and GLP-1 secretion and GPCRs involved in enteroendocrine cell signalling

Author:
Marta Santos-Hernández; Santiaga María Vivanco-Maroto; Beatriz Miralles; Isidra Recio
Source:
Food chemistry 2023 v.402 pp. 134225
ISSN:
0308-8146
Subject:
G-proteins; calcium receptors; cell lines; cholecystokinin; digestion; food chemistry; gastrointestinal system; glucagon-like peptide 1; hormone secretion; lysozyme
Abstract:
... The strong effect of protein digestion products on gastrointestinal-released hormones is recognised. However, little is known about the specific peptide sequences able to induce gastrointestinal hormone secretion and the receptors involved. Our objective was to identify peptides able to induce the secretion of cholecystokinin (CCK) and glucagon like peptide-1 (GLP-1) in the enteroendocrine cell li ...
DOI:
10.1016/j.foodchem.2022.134225

7. Metabolomics driven analysis of Nigella sativa seeds identifies the impact of roasting on the chemical composition and immunomodulatory activity

Author:
Mohamed A. Salem; Riham A. El-Shiekh; Nora M. Aborehab; Ahmed A. Al‐Karmalawy; Shahira M. Ezzat; Saleh Alseekh; Alisdair R. Fernie
Source:
Food chemistry 2023 v.398 pp. 133906
ISSN:
0308-8146
Subject:
Nigella sativa; cell lines; chemical composition; flavonoids; flavor; food chemistry; immunomodulators; metabolites; metabolomics; monocytes; saponins
Abstract:
... Nigella sativa L. seeds (NS) are known as one of the most traditional immunomodulatory and nutritive food additives. NS can, furthermore, be roasted to give curries, breads, and other dishes a smoky, nutty flavor. This study evaluated the effect of roasting NS on the metabolic profile and immunomodulatory activity. Non-targeted metabolomics analysis was conducted using several analytical platforms ...
DOI:
10.1016/j.foodchem.2022.133906

8. N-acetylation of toxic aromatic amines by fungi: Strain screening, cytotoxicity and genotoxicity evaluation, and application in bioremediation of 3,4-dichloroaniline

Author:
Amanda Dal’Ongaro Rodrigues; Arthur dos Santos Montanholi; Angela Akimi Shimabukuro; Murilo Kioshi Aquino Yonekawa; Nadla Soares Cassemiro; Denise Brentan Silva; Clarice Rossato Marchetti; Carlos Eduardo Weirich; Adilson Beatriz; Fabiana Fonseca Zanoelo; Maria Rita Marques; Giovana Cristina Giannesi; Silvia Cordeiro das Neves; Rodrigo Juliano Oliveira; Roberto Ruller; Dênis Pires de Lima; Edson dos Anjos dos Santos
Source:
Journal of hazardous materials 2023 v.441 pp. 129887
ISSN:
0304-3894
Subject:
Aspergillus niveus; Aspergillus terreus; Chaetomium; Cladosporium cladosporioides; Lactuca sativa; Mucor; bioremediation; biotransformation; cell lines; colorimetry; cytotoxicity; fungi; genotoxicity; humans; industry; keratinocytes; mutagens; phenylurea herbicides; phytotoxicity; soil
Abstract:
... Aromatic amines (AA) are one of the most commonly used classes of compounds in industry and the most common pollutants found in both soil and water. 3,4-Dichloaniline (3,4-DCA) is a persistent residue of the phenylurea herbicide in the environment. In this study, we used a colorimetric method as a new approach to screen 12 filamentous fungal strains of the genera Aspergillus, Chaetomium, Cladospor ...
DOI:
10.1016/j.jhazmat.2022.129887

9. 2‐Arachidonoyl glycerol suppresses gastric emptying via the cannabinoid receptor 1‐cholecystokinin signaling pathway in mice

Author:
Keita Ochiai; Rina Hirooka; Masayoshi Sakaino; Shigeo Takeuchi; Tohru Hira
Source:
Lipids 2022 v.57 no.3 pp. 173-181
ISSN:
0024-4201
Subject:
absorption; acetaminophen; antagonists; cannabinoid receptors; cell lines; cholecystokinin; cholecystokinin receptors; glycerol; intraperitoneal injection; mice; phenolsulfonphthalein; secretion
Abstract:
... 2‐Monoacylglycerol (2‐MAG) is one of the digestion products of dietary lipids. We recently demonstrated that a 2‐MAG, 2‐arachidonoyl glycerol (2‐AG) potently stimulated cholecystokinin (CCK) secretion via cannabinoid receptor 1 (CB1) in a murine CCK‐producing cell line, STC‐1. CCK plays a crucial role in suppressing postprandial gastric emptying. To examine the effect of 2‐AG on gastric emptying, ...
DOI:
10.1002/lipd.12341

10. 3,4,5-O-tricaffeoylquinic acid with anti-radiation activity suppresses LPS-induced NLRP3 inflammasome activation via autophagy in THP-1 macrophages

Author:
Jiajun Liu; Jingyun Chen; Baixue Xu; Long Lin; Shaoqun Liu; Xiaoying Ma; Jianwen Liu
Source:
Molecular immunology 2022 v.147 pp. 187-198
ISSN:
0161-5890
Subject:
Azolla; adverse effects; autophagosomes; autophagy; caspase-1; cell lines; cesium radioisotopes; chloroquine; flow cytometry; humans; immune response; inflammasomes; inflammation; lipopolysaccharides; macrophages; proteasome inhibitors; radiotherapy; secretion
Abstract:
... Damage to normal tissues caused by excessive ionizing radiation (IR) exposure is the major side effect of radiotherapy. Several recent studies have shown that IR-induced damage to tissues leads to a systemic immune response and NLRP3 inflammasome activation in immune cells. 3,4,5-O-tricaffeoylquinic acid (tCQA), extracted from the natural plant Azolla imbricata, relieves inflammation and has radio ...
DOI:
10.1016/j.molimm.2022.05.011

11. 3,4,5‐O‐tricaffeoylquinic acid alleviates ionizing radiation‐induced injury in vitro and in vivo through regulating ROS/JNK/p38 signaling

Author:
Jiajun Liu; Wen Hu; Xiaoying Ma; Xin Liang; Long Lin; Jianming Huang; Jianwen Liu
Source:
Environmental toxicology 2022 v.37 no.2 pp. 349-361
ISSN:
1520-4081
Subject:
Azolla; Western blotting; agonists; apoptosis; caspases; cell lines; cytotoxicity; digestive system; ecotoxicology; epithelial cells; immune system; membrane potential; mitochondria; radioprotective effect; radiotherapy
Abstract:
... Ionizing radiation (IR) brings many health problems to humans, causing damage to the digestive system, hematopoietic system, and immune system. Natural compounds derived from plants have attracted widespread attention due to their low toxicity. Here, we found that 3,4,5‐O‐tricaffeoylquinic acid (tCQA) extracted from natural plant Azolla imbricata could significantly alleviate the systemic damage i ...
DOI:
10.1002/tox.23403

12. 7-Hydroxy-2-octenoic acid-ethyl ester mixture as an UV protectant secondary metabolite of an endolichenic fungus isolated from Menegazzia terebrata

Author:
Lu Zhao; Jin-Cheol Kim; Jae-Seoun Hur
Source:
Archives of microbiology 2022 v.204 no.7 pp. 395
ISSN:
0302-8933
Subject:
Menegazzia; absorption; ascorbic acid; cell lines; cytotoxicity; fungi; irradiation; linoleic acid; melanin; melanoma; mice; peroxidation; secondary metabolites; superoxide anion
Abstract:
... Enodolichenic fungi (ELF) are considered a promising bio-resource since they produce a variety of novel secondary metabolites with bioactivities. Ultraviolet (UV) radiation in sunlight containing UVA and UVB can cause acute and chronic skin diseases, and the demand for UV protectants in sunscreens has been increasing. Such situations evoke the strong interest of researchers in seeking effective UV ...
DOI:
10.1007/s00203-022-02997-5

13. 7-Hydroxycoumarin mitigates the severity of collagen-induced arthritis in rats by inhibiting proliferation and inducing apoptosis of fibroblast-like synoviocytes via suppression of Wnt/β-catenin signaling pathway

Author:
Li Cai; Pan Zong; Meng-yuan Zhou; Fang-yuan Liu; Bo Meng; Ming-ming Liu; Zeng Li; Rong Li
Source:
Phytomedicine 2022 v.94 pp. 153841
ISSN:
0944-7113
Subject:
anti-inflammatory activity; antibodies; apoptosis; blood serum; caspase-3; cell lines; cell proliferation; collagen; coumarin; cyclins; immunohistochemistry; interleukin-6; lithium chloride; mitochondria; protective effect; protein content; rats; rheumatoid arthritis; therapeutics; umbelliferones; wnt proteins
Abstract:
... 7-Hydroxycoumarin (7-HC) as a coumarin compound is widely found in Chinese herbs and exhibits diverse biological activities. Promoting cell apoptosis of fibroblast-like synoviocytes (FLS) is a meaningful strategy for rheumatoid arthritis (RA). Though the protective effect of 7-HC on RA experimental models has been reported, the specific mechanisms, especially the possible relationships of this eff ...
DOI:
10.1016/j.phymed.2021.153841

14. In vitro transmission of Chlamydia using naturally infected koala (Phascolarctos cinereus) semen

Author:
Lyndal Hulse; Kenneth W. Beagley; Rebecca Larkin; Stephen D. Johnston
Source:
Reproduction, fertility, and development 2022 v.34 no.3 pp. 378-384
ISSN:
1031-3613
Subject:
Chlamydia; Chlamydophila pecorum; Phascolarctos cinereus; cell lines; immune response; quantitative polymerase chain reaction; reproduction; semen
Abstract:
... Transmission of Chlamydia pecorum infection has generally been assumed to be via the urogenital route and in an attempt to confirm this we investigated an in vitro method of Chlamydia infection using naturally infected koala semen to inoculate a cell line and attempt to estimate C. pecorum infectious load. A total of 57% of 122 koala semen samples had low C. pecorum copy number or no burden, while ...
DOI:
10.1071/RD21233

15. APE1 controls DICER1 expression in NSCLC through miR-33a and miR-130b

Author:
Giulia Antoniali; Emiliano Dalla; Giovanna Mangiapane; Xiaolong Zhao; Xinming Jing; Yi Cheng; Veronica De Sanctis; Dilara Ayyildiz; Silvano Piazza; Mengxia Li; Gianluca Tell
Source:
Cellular and molecular life sciences 2022 v.79 no.8 pp. 446
ISSN:
1420-682X
Subject:
DNA repair; biogenesis; biomarkers; carcinogenesis; cell lines; cell proliferation; drug therapy; gene expression regulation; humans; lung neoplasms; microRNA; neoplasm cells
Abstract:
... Increasing evidence suggests different, not completely understood roles of microRNA biogenesis in the development and progression of lung cancer. The overexpression of the DNA repair protein apurinic/apyrimidinic endodeoxyribonuclease 1 (APE1) is an important cause of poor chemotherapeutic response in lung cancer and its involvement in onco-miRNAs biogenesis has been recently described. Whether AP ...
DOI:
10.1007/s00018-022-04443-7

16. AR independent anticancer potential of enza against prostate cancer

Author:
Abu Baker; Asad Syed; Sana Iram; Abdallah M. Elgorban; Helal F. Al-Harthi; Salim S. Al-Rejaie; Jihoe Kim; Mohd Sajid Khan
Source:
Colloids and surfaces 2022 v.642 pp. 128598
ISSN:
0927-7757
Subject:
apoptosis; biosynthesis; cell lines; cell viability; drugs; membrane potential; metastasis; nanogold; patient compliance; patients; pro-apoptotic proteins; prostatic neoplasms; survival rate; synergism
Abstract:
... The survival rate of metastatic castrate resistance prostate cancer (CRPC) patient can be improved by the second-generation anti-androgen drugs such as enzalutamide (enza). But unfortunately, CRPC patients do respond with enza in the beginning and become resistance in due course of time against this drug. Therefore, in the given investigation, enza was delivered to the AR-null cells (PC3 & DU145) ...
DOI:
10.1016/j.colsurfa.2022.128598

17. Accurate identification of circRNA landscape and complexity reveals their pivotal roles in human oligodendroglia differentiation

Author:
Yangping Li; Feng Wang; Peng Teng; Li Ku; Li Chen; Yue Feng; Bing Yao
Source:
Genome biology 2022 v.23 no.1 pp. 48
ISSN:
1474-760X
Subject:
brain; cell lines; exons; gene expression; humans; introns; landscapes; microRNA; neurons; oligodendroglia; ribonucleases
Abstract:
... BACKGROUND: Circular RNAs (circRNAs), a novel class of poorly conserved non-coding RNAs that regulate gene expression, are highly enriched in the human brain. Despite increasing discoveries of circRNA function in human neurons, the circRNA landscape and function in developing human oligodendroglia, the myelinating cells that govern neuronal conductance, remains unexplored. Meanwhile, improved expe ...
DOI:
10.1186/s13059-022-02621-1

18. Acetate, a metabolic product of Heligmosomoides polygyrus, facilitates intestinal epithelial barrier breakdown in a FFAR2-dependent manner

Author:
Fabian Schälter; Michael Frech; Kerstin Dürholz; Sébastien Lucas; Kerstin Sarter; Luc Lebon; Julia Esser-von Bieren; Lalit K. Dubey; David Voehringer; Georg Schett; Nicola L. Harris; Mario M. Zaiss
Source:
International journal for parasitology 2022 v.52 no.9 pp. 591-601
ISSN:
0020-7519
Subject:
G-proteins; Heligmosomoides polygyrus; acetates; cell lines; energy metabolism; epithelial cells; epithelium; free fatty acids; helminths; intestines; livestock; organoids; parasitology; permeability; reaction kinetics; tight junctions
Abstract:
... Approximately 2 billion people worldwide and a significant part of the domestic livestock are infected with soil-transmitted helminths, of which many establish chronic infections causing substantial economic and welfare burdens. Beside intensive research on helminth-triggered mucosal and systemic immune responses, the local mechanism that enables infective larvae to cross the intestinal epithelial ...
DOI:
10.1016/j.ijpara.2022.04.004

19. Achieving robust somatic mutation detection with deep learning models derived from reference data sets of a cancer sample

Author:
Sayed Mohammad Ebrahim Sahraeian; Li Tai Fang; Konstantinos Karagiannis; Malcolm Moos; Sean Smith; Luis Santana-Quintero; Chunlin Xiao; Michael Colgan; Huixiao Hong; Marghoob Mohiyuddin; Wenming Xiao
Source:
Genome biology 2022 v.23 no.1 pp. 12
ISSN:
1474-760X
Subject:
DNA; DNA damage; cell lines; computer simulation; gene frequency; genome; genomics; models; neoplasm cells; neoplasms; somatic mutation
Abstract:
... BACKGROUND: Accurate detection of somatic mutations is challenging but critical in understanding cancer formation, progression, and treatment. We recently proposed NeuSomatic, the first deep convolutional neural network-based somatic mutation detection approach, and demonstrated performance advantages on in silico data. RESULTS: In this study, we use the first comprehensive and well-characterized ...
DOI:
10.1186/s13059-021-02592-9

20. Aconitine induces cell apoptosis via mitochondria and death receptor signaling pathways in hippocampus cell line

Author:
Hui Wang; Yanbing Liu; Ziyu Guo; Kexin Wu; Yunhao Zhang; Yanan Tian; Baoyu Zhao; Hao Lu
Source:
Research in veterinary science 2022 v.143 pp. 124-133
ISSN:
0034-5288
Subject:
aconitine; apoptosis; cell lines; death domain receptors; dopamine; dose response; hippocampus; mitochondria; research; toxicity; toxins; veterinary medicine; viability
Abstract:
... Aconitine is a plant toxin derived from aconitum genus and well known for its neurological and vascular toxicity. However, the mechanism of toxicity on the growth and apoptosis of the neurological cells has not been well investigated. In this study, we used HT22 cell lines derived from hippocampus to explore the mechanism. We began with examination of the viability and DA (dopamine) contents of ce ...
DOI:
10.1016/j.rvsc.2022.01.001