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- Author:
- R. llahy; M. W. Siddiqui; G. Piro; M. S. Lenucci; C. Hdider; L. Helyes
- Source:
- Acta horticulturae 2017 no.1159 pp. 57-64
- ISSN:
- 0567-7572
- Subject:
- antioxidants; ascorbic acid; beta-carotene; breeding programs; brix; chemical elements; crop yield; cultivars; environmental factors; flavonoids; fruits; genetically modified organisms; health promotion; horticulture; introgression; leaves; lycopene; mutants; phenolic compounds; phytopharmaceuticals; pigmentation; pleiotropy; titratable acidity; tomatoes; total soluble solids; Mediterranean region
- Abstract:
- ... Both transgenic and conventional breeding methods have been used to increase levels and diversify the profile of antioxidants in ripe tomato fruit. The high-pigment (hp) tomato mutants exemplify this approach, representing useful genetic resources to be introgressed into elite cultivars (high-lycopene cultivars) to simultaneously enhance the levels of different classes of antioxidants. Although se ...
- DOI:
- 10.17660/ActaHortic.2017.1159.9
- https://doi.org/10.17660/ActaHortic.2017.1159.9
- Author:
- Kai Song; Yiqun Xue; Xiaohua Wang; Yinglang Wan; Xin Deng; Jinxing Lin
- Source:
- Journal of plant physiology 2017 v.213 pp. 129-133
- ISSN:
- 0176-1617
- Subject:
- Arabidopsis thaliana; chemical elements; dimerization; fluorescence; genetically modified organisms; image analysis; membrane proteins; mutation; photobleaching; protein subunits
- Abstract:
- ... Membrane proteins exert functions by forming oligomers or molecular complexes. Currently, step-wise photobleaching has been applied to count the fluorescently labelled subunits in plant cells, for which an accurate and reliable control is required to distinguish individual subunits and define the basal fluorescence. However, the common procedure using immobilized GFP molecules is obviously not app ...
- DOI:
- 10.1016/j.jplph.2017.03.009
- https://doi.org/10.1016/j.jplph.2017.03.009
- Author:
- Lauro Bücker Neto; Rafael Augusto Arenhart; Luiz Felipe Valter de Oliveira; Júlio Cesar de Lima; Maria Helena Bodanese-Zanettini; Rogerio Margis; Márcia Margis-Pinheiro
- Source:
- Plant cell reports 2015 v.34 no.11 pp. 1899-1907
- ISSN:
- 0721-7714
- Subject:
- rice; chemical elements; seedlings; roots; genetically modified organisms; regulator genes; assays; gene expression; microRNA; transcription factors; transcriptomics; plant development; high-throughput nucleotide sequencing; quantitative polymerase chain reaction; protoplasts; transcriptional activation; stress response; reverse transcriptase polymerase chain reaction; binding sites; ripening; abscisic acid
- Abstract:
- ... KEY MESSAGE : The work describes an ASR knockdown transcriptomic analysis by deep sequencing of rice root seedlings and the transactivation of ASR cis-acting elements in the upstream region of a MIR gene. MicroRNAs are key regulators of gene expression that guide post-transcriptional control of plant development and responses to environmental stresses. ASR (ABA, Stress and Ripening) proteins are p ...
- DOI:
- 10.1007/s00299-015-1836-3
- https://doi.org/10.1007/s00299-015-1836-3
- Author:
- Peng, Zhi-yu; Zhou, Xin; Li, Linchuan; Yu, Xiangchun; Li, Hongjiang; Jiang, Zhiqiang; Cao, Guangyu; Bai, Mingyi; Wang, Xingchun; Jiang, Caifu; Lu, Haibin; Hou, Xianhui; Qu, Lijia; Wang, Zhiyong; Zuo, Jianru; Fu, Xiangdong; Su, Zhen; Li, Songgang; Guo, Hongwei
- Source:
- Nucleic acids research 2009 v.37 no.suppl_1 pp. D975
- ISSN:
- 0305-1048
- Subject:
- Arabidopsis; Internet; abscisic acid; auxins; brassinosteroids; chemical elements; cytokinins; ethylene; gene ontology; genes; genetically modified organisms; gibberellins; growth and development; hormonal regulation; jasmonic acid; microarray technology; mutants; nucleic acids; phenotype; salicylic acid
- Abstract:
- ... Plant hormones are small organic molecules that influence almost every aspect of plant growth and development. Genetic and molecular studies have revealed a large number of genes that are involved in responses to numerous plant hormones, including auxin, gibberellin, cytokinin, abscisic acid, ethylene, jasmonic acid, salicylic acid, and brassinosteroid. Here, we develop an Arabidopsis hormone data ...
- Author:
- René Köppel; Verena Peterseil; Emilie Dagand; Ekkehard Schütz; Nina Kolberg; Mojca Milavec; Dominik Moor
- Source:
- European food research & technology 2017 v.243 no.6 pp. 1091-1096
- ISSN:
- 1438-2377
- Subject:
- DNA; chemical elements; equipment; genetically modified organisms; lectins; polymerase chain reaction; transgenes
- Abstract:
- ... Digital PCR promises absolute quantification of amplifiable nucleic acids molecules. In consequence, results should be comparable, irrespective of the analysing laboratory and the equipment used. To assess this claim, we used a multitarget DNA molecule (amplicon) encoding for eight transgene soy traits and the lectin soy reference gene. This multitarget amplicon was analysed by seven European labo ...
- DOI:
- 10.1007/s00217-016-2824-8
- https://doi.org/10.1007/s00217-016-2824-8
- Author:
- Amina Antonacci; Ivo Bertalan; Maria Teresa Giardi; Viviana Scognamiglio; Mehmet Turemis; Dirk Fisher; Udo Johanningmeier
- Source:
- Algal research 2021 v.54 pp. 102184
- ISSN:
- 2211-9264
- Subject:
- Chlamydomonas reinhardtii; D1 protein; Macruronus novaezelandiae; algae; antioxidant activity; antioxidants; bioassays; chemical elements; chloroplasts; design; detection; fish; genetic engineering; genetically modified organisms; heat stress; herbicides; light; models; mutants; oxidative stress; peptides; photosystem II; research; shelf life; strains; vision
- Abstract:
- ... Algae emerge as leading actors for the development of new strategies to cope oxidative stress, thanks to their availability for genetic manipulation and simple cultivation. This study reports the design of novel mutant strains of the green photosynthetic alga Chlamydomonas reinhardtii, as a model system, able to produce bioactive peptides with high antioxidant capacity by means of a custom-made ex ...
- DOI:
- 10.1016/j.algal.2021.102184
- https://doi.org/10.1016/j.algal.2021.102184
- Author:
- Pluhackova Kristyna; Morhenn Humphrey; Lautner Lisa; Lohstroh Wiebke; Nemkovski Kirill S.; Unruh Tobias; Böckmann Rainer A.
- Source:
- Journal of physical chemistry 2015 v.119 no.49 pp. 15287-15299
- ISSN:
- 1520-5207
- Subject:
- alcohols; chemical elements; esters; genetically modified organisms; lipids; models; molecular dynamics; volatilization
- Abstract:
- ... The recently presented LOPLS-AA all-atom force field for long hydrocarbon chains, based on the OPLS-AA force field, was extended to alcohols, esters, and glyceryl monooleate (GMO) lipids as a model lipid. Dihedral angles were fitted against high level ab initio calculations, and ester charges were increased to improve their hydration properties. Additionally, the ester Lennard-Jones parameters wer ...
- DOI:
- 10.1021/acs.jpcb.5b08569
- https://doi.org/10.1021/acs.jpcb.5b08569
- Author:
- Shoukun Chen; Yunxin Luo; Guojing Wang; Cuizhu Feng; Haifeng Li
- Source:
- Plant science 2020 v.296 pp. 110490
- ISSN:
- 0168-9452
- Subject:
- Arabidopsis; Brachypodium distachyon; abiotic stress; chemical elements; chromosomes; expansins; genes; genetically modified organisms; germination; length; phylogeny; plant development; plant growth; root hairs; stress response; structural proteins; width
- Abstract:
- ... Plant expansin belongs to a group of cell wall proteins and functions in plant growth and development. However, limited data are available on the contributions of expansins in Brachypodium distachyon. In the present study, a total of 38 expansins were identified in B. distachyon genome. Phylogenetic analysis divided the expansins into four groups, namely EXPA, EXPB, EXLA, and EXLB. Chromosomal dis ...
- DOI:
- 10.1016/j.plantsci.2020.110490
- https://doi.org/10.1016/j.plantsci.2020.110490
- Author:
- Shuang Qiu; Jun Zhang; Jiaqi He; Wei Sha; Mingyang Li; Yan Zhao; Ying Zhai
- Source:
- Plant cell, tissue, and organ culture 2020 v.143 no.3 pp. 507-516
- ISSN:
- 0167-6857
- Subject:
- amino acid sequences; binding sites; catalytic activity; chemical elements; cold stress; drought tolerance; electrolyte leakage; fluorescence; genes; genetically modified organisms; hydrophobicity; inositol 3-alpha-galactosyltransferase; malondialdehyde; organ culture; plant adaptation; polypeptides; proline; promoter regions; proteins; quantitative polymerase chain reaction; raffinose; soybeans; synthesis; tobacco; water stress
- Abstract:
- ... Plant adaptation to abiotic stress is significantly influenced by galactinol synthase (GolS), which is a regulatory enzyme that catalyzes the synthesis of raffinose family oligosaccharides (RFOs). Soybean GolS genes, GmGolS2-1 and GmGolS2-2 were isolated in this study. GmGolS2-1 was 993 bp and encoded a 330-amino acid polypeptide, and GmGolS2-2 was 975 bp and encoded a 324-amino acid polypeptide. ...
- DOI:
- 10.1007/s11240-020-01936-w
- https://doi.org/10.1007/s11240-020-01936-w
- Author:
- Kazuyoshi Kitazaki; Takumi Arakawa; Muneyuki Matsunaga; Rika Yui‐Kurino; Hiroaki Matsuhira; Tetsuo Mikami; Tomohiko Kubo
- Source:
- plant journal 2015 v.83 no.2 pp. 290-299
- ISSN:
- 0960-7412
- Subject:
- cytoplasmic male sterility; Beta vulgaris; genetically modified organisms; immunoblotting; sugar beet; RNA-binding proteins; chemical elements; translation (genetics); open reading frames; polyacrylamide gel electrophoresis; genes; mitochondria; pollen; callus
- Abstract:
- ... Genetic conflict between cytoplasmically inherited elements and nuclear genes arising from their different transmission patterns can be seen in cytoplasmic male sterility (CMS), the mitochondrion‐encoded inability to shed functional pollen. CMS is associated with a mitochondrial open reading frame (ORF) that is absent from non‐sterility inducing mitochondria (S‐orf). Nuclear genes that suppress CM ...
- DOI:
- 10.1111/tpj.12888
- https://doi.org/10.1111/tpj.12888
- Author:
- Giovanni Giuliano
- Source:
- Current opinion in biotechnology 2017 v.44 pp. 169-180
- ISSN:
- 0958-1669
- Subject:
- antioxidants; beta-carotene; bioavailability; biofortification; breeding; chemical elements; crops; developing countries; genetically modified organisms; healthy diet; photosynthesis; pigments; plant-based foods; rice; vitamin A; vitamin A deficiency; vitamin status
- Abstract:
- ... Carotenoids are synthesized de novo by plants, where they play fundamental physiological roles as photosynthetic pigments and precursors for signaling molecules. They are also essential components of a healthy diet, as dietary antioxidants and vitamin A precursors. Vitamin A deficiency is a public health problem in developing countries, which has prompted a series of efforts toward the biofortific ...
- DOI:
- 10.1016/j.copbio.2017.02.001
- https://doi.org/10.1016/j.copbio.2017.02.001
- Author:
- Eva Zupančič; Caterina Curato; Maria Paisana; Catarina Rodrigues; Ziv Porat; Ana S. Viana; Carlos A.M. Afonso; João Pinto; Rogério Gaspar; João N. Moreira; Ronit Satchi-Fainaro; Steffen Jung; Helena F. Florindo
- Source:
- Journal of Controlled Release 2017 v.258 pp. 182-195
- ISSN:
- 0168-3659
- Subject:
- CD8-positive T-lymphocytes; Toll-like receptors; alcohols; animals; antigens; chemical elements; dendritic cells; gene expression regulation; genetically modified organisms; immune response; infectious diseases; ligands; lymph nodes; major histocompatibility complex; models; nanoparticles; neoplasms; oligodeoxyribonucleotides; ovalbumin; phenotype; polymers; surfactants; vaccination
- Abstract:
- ... Vaccination is a promising strategy to trigger and boost immune responses against cancer or infectious disease. We have designed, synthesized and characterized aliphatic-polyester (poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NP) to investigate how the nature of protein association (adsorbed versus entrapped) and polymer/surfactant concentrations impact on the generation and modulation of a ...
- DOI:
- 10.1016/j.jconrel.2017.05.014
- https://doi.org/10.1016/j.jconrel.2017.05.014
- Author:
- Roy Bibhisan; Satpathi Sagar; Gavvala Krishna; Koninti Raj Kumar; Hazra Partha
- Source:
- Journal of physical chemistry 2015 v.119 no.35 pp. 11721-11731
- ISSN:
- 1520-5207
- Subject:
- X-radiation; activation energy; chemical elements; cylinders; diffusivity; drugs; encapsulation; genetically modified organisms; glycerol; light microscopy; nuclear magnetic resonance spectroscopy; phase transition
- Abstract:
- ... Reverse hexagonal (HII) liquid crystalline material based on glycerol monooleate (GMO) is considered as a potential carrier for drugs and other important biomolecules due to its thermotropic phase change and excellent morphology. In this work, the dynamics of encapsulated water, which plays important role in stabilization and formation of reverse hexagonal mesophase, has been investigated by time ...
- DOI:
- 10.1021/acs.jpcb.5b04370
- https://doi.org/10.1021/acs.jpcb.5b04370
14. The overexpression of rice ACYL-COA-BINDING PROTEIN4 improves salinity tolerance in transgenic rice
- Author:
- Ze-Hua Guo; Gorana Pogancev; Wei Meng; Zhi-Yan Du; Pan Liao; Rui Zhang; Mee-Len Chye
- Source:
- Environmental and experimental botany 2021 v.183 pp. 104349
- ISSN:
- 0098-8472
- Subject:
- Arabidopsis; acyl coenzyme A; assays; calorimetry; chemical elements; climate change; crop production; esters; fatty acid composition; fatty acids; gel electrophoresis; genes; genetically modified organisms; leaves; lipid metabolism; rice; salinity; salt stress; salt tolerance; seedlings; sequence analysis; tissues; titration; vector control
- Abstract:
- ... Increased salinity, as consequence of the ongoing climate change, has a significant impact on crop production worldwide. The overexpression of rice ACYL-COA-BINDING PROTEIN4 (OsACBP4) protects transgenic rice and Arabidopsis from salinity stress, while OsACBP4-RNAi lines were more susceptible than the vector control (VC) and wild type (WT). The overexpression of AtACBP2, the Arabidopsis OsACBP4 ho ...
- DOI:
- 10.1016/j.envexpbot.2020.104349
- https://doi.org/10.1016/j.envexpbot.2020.104349
- Author:
- Wouter Kohlen; Tatsiana Charnikhova; Michiel Lammers; Tobia Pollina; Peter Tóth; Imran Haider; María J. Pozo; Ruud A. de Maagd; Carolien Ruyter‐Spira; Harro J. Bouwmeester; Juan A. López‐Ráez
- Source:
- new phytologist 2012 v.196 no.2 pp. 535-547
- ISSN:
- 0028-646X
- Subject:
- Orobanche ramosa; Solanum lycopersicum; adventitious roots; apical dominance; biosynthesis; branching; carotenoids; chemical elements; flowers; fruit yield; fruits; genetically modified organisms; parasitic plants; phenotype; plant architecture; rhizosphere; sap; secretion; seeds; strigolactones; tomatoes; vesicular arbuscular mycorrhizae; weed control; xylem
- Abstract:
- ... Strigolactones are plant hormones that regulate both above‐ and belowground plant architecture. Strigolactones were initially identified as rhizosphere signaling molecules. In the present work, the tomato (Solanum lycopersicum) CAROTENOID CLEAVAGE DIOXYGENASE 8 (SlCCD8) was cloned and its role in rhizosphere signaling and plant physiology assessed by generating knock‐down lines. Transgenic SlCCD8 ...
- DOI:
- 10.1111/j.1469-8137.2012.04265.x
- https://doi.org/10.1111/j.1469-8137.2012.04265.x
- Author:
- A. Hua-Van; J.A. Pamphile; T. Langin; M.-J. Daboussi
- Source:
- MGG 2001 v.264 no.5 pp. 724-731
- ISSN:
- 0026-8925
- Subject:
- Aepyceros melampus; Fusarium oxysporum; chemical elements; fungi; genetically modified organisms; genomics; hygromycin B; nitrate reductase; resistance genes; strains; transcriptional activation; transposases; transposons
- Abstract:
- ... The impala transposon of Fusarium oxysporum is an active element. We demonstrated that the imp160 copy, transposed into the gene encoding nitrate reductase, is an autonomous element, since it excises from this gene and reinserts at a new genomic position in backgrounds free of active elements. An element in which the transposase gene was replaced by a hygromycin B resistance gene was used (1) to d ...
- DOI:
- 10.1007/s004380000395
- https://doi.org/10.1007/s004380000395