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... This work aims to investigate antifungal, mycotoxin inhibitory efficacy of the hop essential oil (HEO) nanoemulsion and their mode of action (MOA) against Fusarium graminearum isolate, a fungal pathogen causing Fusarium Head Blight (FHB) in cereal crops. The HEO, primarily consisting of terpenes and terpenoids, was encapsulated in nanoemulsion droplets. Physically stable HEO-in-water nanoemulsion ...
... Carbohydrate-binding modules (CBMs) are commonly found within chitinases, but their contributions to chitinolytic systems are poorly understood. To address this knowledge gap, full-length chitin-acting enzymes (ChiA, ChiB, ChiC, and CBP21) of Serratia marcescens BWL1001 and CBM-truncated versions (ChiB-dCBM and ChiC-dCBM) were heterologously expressed for enzymological analysis. The CBM5 of ChiB a ...
... In this work, a sequential fractionation protocol (cold and hot aqueous and alkaline extractions) and detailed compositional analysis (gross composition, monosaccharide analysis, FTIR, TGA) was applied to three relevant mushrooms in terms of global production (Grifola frondosa, Lentinula edodes and Pleurotus ostreatus) aiming to understand what is preferentially extracted during fractionation and ...
chitin; deformation; evolution; gel strength; gels; hydrocolloids; microstructure; nanocrystals; nuclear magnetic resonance spectroscopy; pH; shrimp; soy protein isolate; transmission electron microscopy; viscoelasticity
Abstract:
... Chitin nanocrystals (ChNs) were successfully fabricated from shrimp and their effects on the microstructure and gelling properties of glucono-δ-lactone-induced soy protein isolate (SPI) were investigated. Firstly, transmission electron microscopy (TEM) showed that the average length and width of ChNs were 208.8 nm and 19.2 nm, respectively. The zeta potentials of ChNs changed from +38.6 mV at pH 3 ...
... Presented here is the synthesis of a 3D printable nano-polysaccharide self-healing hydrogel for flexible strain sensors. Consisting of three distinct yet complementary dynamic bonds, the crosslinked network comprises imine, hydrogen, and catecholato-metal coordination bonds. Self-healing of the hydrogel is demonstrated by macroscopic observation, rheological recovery, and compression measurements. ...
... BACKGROUND: In the recent epoch, chitinolytic enzymes have attained much attention as promising biocontrol agents and ability to convert recalcitrant chitinous wastes into valuable products. Some chitinolytic microorganisms have emerged as the potential candidate to carry a noticeable ability to degrade this polysaccharide in nature. OBJECTIVE: Therefore, in this study, a new bacterial strain Acin ...
... Most natural polymers exhibit limited functional groups, which is not favourable for the adsorption of various ions and their utilisation. To overcome this drawback, a novel in-situ-doped nano-calcium carbonate (CaCO₃) chitin hydrogel was synthesised as an efficient adsorbent for Cu (II) and Cd (II) ions. Scanning electron microscopy and Brunauer–Emmett–Teller results revealed that the synthesised ...
... Removal of the premetalized dyes from the effluents of textile and leather industries is still open. The work aims to investigate functional groups taking part in the sorption of premetalized dye Acid Blue 158:Cr on chitin/chitosan and to investigate the mechanism of the sorption. The process was spontaneous (negative value of the Gibbs function), endothermic (ΔH = 0.2679 kJ/mol) with an affinity ...
... Chitosan was prepared by hydrothermal deacetylation from multi-step protein purification chitin based on Trypoxylus dichotomus, for treating heavy metals and antibiotics. Chitosan with higher deacetylation degree and lower molecular weight were synthesized. The adult chitosan was composed of nanofibers arranged more evenly, showing higher yield, thermal stabilities and antimicrobial properties. Th ...
... Gut microbiome homeostasis is critical in preventing diseases. However, the effect of disease on gut microbiota assembly remains unclear. At present, there are no reports on the composition and functional analysis of intestinal microbiota of Indian major carp, rohu (L. rohita) infected with ectoparasite, Argulus. In this study, we analysed and compared the intestinal microbiota of healthy and Argu ...
... Dry rot is a major disease of potato during storage, which is caused by several Fusarium species. Biological control based on natural bioactive compounds that inhibit tuber disease provides an attractive alternative because they are ecofriendly and low toxic. In this study, a monoterpene phenol thymol (2-isopropyl-5-methylphenol) was found to have an effective antifungal effect against Fusarium ox ...
... This study aimed to examine the antifungal activity of wild bergamot (Monarda fistulosa) essential oil on Colletotrichum musae and Lasiodiplodia theobromae, the causative organisms of anthracnose and crown-rot diseases of banana fruits, respectively. Chitin synthase is a promising target for antifungal compounds, since it is involved in synthesizing chitin, which forms a major proportion of the fu ...
Tareq M. Osaili; Anas A. Al‐Nabulsi; Fayeza Hasan; Amin N. Olaimat; Sadi Taha; Mutamed Ayyash; Dima S. Nazzal; Ioannis N. Savvaidis; Reyad S. Obaid; Richard Holley
... The study aimed to evaluate the antimicrobial activity of 0.5 or 1% (w/w) chitosan and 1% (w/w) garlic against Salmonella spp., Escherichia coli O157:H7 and Listeria monocytogenes in hummus dip stored at 4, 10, or 25°C for 28, 21, or 7 days, respectively. In hummus without garlic, at all storage temperatures and storage periods, 0.5% chitosan decreased Salmonella spp., E. coli O157:H7, and L. mono ...
chemical constituents of plants; chitin; color; diet; entomologists; ethanol; hardness; insects; phenols; phytochemicals; secondary metabolites
Abstract:
... Phenolic compounds are a natural product of plants, many of which are foraged by insects and used for different purposes, e.g., defense against foraging, chitin hardness, antioxidants, pigment production, and antimicrobial synthesis. We extracted metabolite ethanol from P. beltrani, quantifying the total phenol content and determining its antioxidative ability by DPPH, ABTS, and FRAP assays. UPLC ...
Eva Tejedor-Calvo; Sergi García-Barreda; Sergio Sánchez; Asunción Morte; María de las Nieves Siles-Sánchez; Cristina Soler-Rivas; Susana Santoyo; Pedro Marco
Terfezia; Tuber aestivum; chitin; ethanol; experimental design; free radicals; fungi; interleukin-6; liquids; principal component analysis; secretion; solvents; sterols; temperature
Abstract:
... A PLE (pressurized liquid extraction) method was adjusted following a full-factorial experimental design to obtain bioactive-enriched fractions from Tuber aestivum and Terfezia claveryi. Temperature, time and solvent (water, ethanol and ethanol–water 1:1) parameters were investigated. The response variables investigated were: obtained yield and the levels of total carbohydrate (compounds, β-glucan ...
... Chitosan material has multiple functions that attract attention from the textile industry, while textile structures make the biocompatible, osteo-conductive and resorbable material promising in a wide range of applications. When the material becomes fibers, the resulting length, strength, fineness and softness would grant the material functions such as structure capacity and a layering system. How ...
biocompatibility; biodegradability; chitin; green chemistry; renewable resources
Abstract:
... As a recyclable and inexhaustible renewable resource, chitin is one of the most abundant polysaccharides in nature. It has excellent biocompatibility, biodegradability, and non-toxicity and has been widely used in food packaging, tissue engineering, and other fields. Deep eutectic solvents (DESs) are regarded as a new generation of green solvents due to their excellent biocompatibility and stabili ...
... Microbial enzymatic degradation of biowaste is a sustainable and environmentally friendly solution for eliminating biowaste pollution. It is the underlying cause of the ever‐increasing demand for harnessing multipurpose microbes to work as an entity under given complex processes. Twelve bacterial strains of bovine manure were evaluated for their hydrolytic enzyme activity and optimization. Six enz ...
... Insects or products derived from insects are considered as a valuable feed ingredient for aquaculture, poultry, and pigs but also exert possible secondary effects of health stimulators in livestock as reported in literature. Health benefits attributed to insects can create additional value in the insect chain. Three categories of bioactive compounds in insects are reported in this desk study: anti ...
agricultural wastes; biochar; carbon; carbon dioxide; cellulose; chitin; green chemistry; hydrochars; life cycle assessment; lignin; neutralization; straw; value added
Abstract:
... Value-added biochar or hydrochar converted from agricultural waste has the potential to improve soil performance. However, how biochar and hydrochar addition affect ecological responses is not clear. Here, we examined the physicochemical property of biochar and hydrochar from straw (RS) and manure (SM), identified relevant eco-friendly pretreatment methods through life cycle assessment (LCA) metho ...
Violah Jepkogei Kemboi; Carolyne Kipkoech; Moses Njire; Samuel Were; Mevin Kiprotich Lagat; Francis Ndwiga; John Mwibanda Wesonga; Chrysantus Mbi Tanga
... Globally, Ralstonia solanacearum (Smith) is ranked one of the most destructive bacterial pathogens inducing rapid and fatal wilting symptoms on tomatoes. Yield losses on tomatoes vary from 0 to 91% and most control measures are unaffordable to resource-poor farmers. This study investigated the antimicrobial activities of chitin and chitosan extracted from black soldier fly (BSF) pupal exuviae agai ...
... The need to increase food production and to reduce the pollution caused by synthetic chemicals has led to a search for biocontrol agents against plant pathogens. In the present study, a total of 37 chitinolytic bacteria were isolated from the rhizospheric soil of tomatoes using a chitin agar medium. In vitro bacterial isolates, that is, TD9, TD11, TD15, and TD24 showed strong antagonistic and enzy ...
... Many biological species combine the helical organization of cellulose or chitin microfibrils with broadband light absorption of black melanin to produce brilliant structural colors with metallic and glossy effects and other diverse functions. In this work, based on core–shell CNC@PDA chiral nanorods consisting of cellulose nanocrystals (CNCs) as the core and melanin-like polydopamine (PDA) as the ...
... Chitinase chi18-5 is an enzyme able to hydrolyze chitin and chitosan producing chitooligosaccharides (COS) of potential technological interest. chi18-5 is produced naturally by the fungus Trichoderma atroviride. It belongs to the glycosyl hydrolase (GH) family 18 of the Carbohydrate Active Enzyme (CAZy) database and it has 83% identity compared to the well-characterized chi42 of Trichoderma harzia ...
Nancy Ley-López; José Basilio Heredia; Cesar San Martín-Hernández; J. Ramón Ibarra-Rodríguez; Miguel Ángel Angulo-Escalante; Raymundo Saúl García-Estrada
... A potential alternative to the use of chemical products with oomyceticidal action for the control of Phytophthora capsici in vegetables is the use of antimicrobial metabolites, biosynthesized in Bacillus species. The objective of this study was to induce the biosynthesis of lipopeptides in Bacillus amyloliquefaciens KX953161.1 by using glutamic acid, iron, cellulose, chitin, or inactive Colletotri ...
... Annually, over 30 million dry tonnes of biosolids are produced worldwide, most of which are disposed into landfills or discharged into waterbodies, exacerbating eutrophication and water-borne diseases. Bioconversion of biosolids using Black Soldier Fly larvae (BSFL) can produce high-value biomass (protein, lipids, and chitin) while reducing the volume of waste that requires disposal. We aimed to d ...
Crustacea; biomass; biorefining; calcium carbonate; chitin; chitosan; green chemistry; human health; research; sustainable development; wastes
Abstract:
... Every year, large amounts of marine biomass waste are generated around the globe. In the case of crustaceans, an opportunity is loss to convert these chemically rich streams into important and industrially relevant materials (e.g., chitin, chitosan, calcium carbonate, proteins, and other nitrogen-containing compounds) as these residues are often landfilled or directly discarded in the environment. ...
... CERK1 (Chitin Elicitor Receptor Kinase 1), a lysin motif‐containing pattern recognition receptor (PRR), perceives chitooligosaccharides (COs) to mount immune and symbiotic responses. However, CERK1, for a relatively long time, has been regarded as a co‐receptor in plant immunity, mainly due to its lack of high binding affinity to known elicitors. Recent studies demonstrated several novel carbohydr ...
... The soil-borne fungus Verticillium dahliae is causing a devastating vascular disease in more than 200 species of dicotyledonous plants. The pathogen attacks susceptible plants through the roots, colonizes the plant vascular system, and causes the death of aerial tissues. In this study, we used Arabidopsis and eggplants to examine the plant protective and immunization effects of autoclaved V. dahli ...
... In recent times, insects have gained attention because of their nutritional characteristics as well as the environmental advantages of their production. In this research, the effect of the diet of grasshoppers (Sphenarium purpurascens) under controlled conditions on their chemical and nutritional content was studied. The insects were divided into two groups: maize leaf-fed grasshoppers (MFG) and s ...
Benjamin M. Rumney; Siân R. Morgan; J. Frederick W. Mosselmans; F. Tegwen Malik; Simon J. Holden; Andrew R. Parker; Nick White; Philip N. Lewis; Julie Albon; Keith M. Meek
... The protective carapace of Skogsbergia lerneri, a marine ostracod, is scratch-resistant and transparent. The compositional and structural organisation of the carapace that underlies these properties is unknown. In this study, we aimed to quantify and determine the distribution of chemical elements and chitin within the carapace of adult ostracods, as well as at different stages of ostracod develop ...
... Halloween genes are involved in the biosynthesis of the molting hormone, which plays a key role in insect ecdysis, development, metamorphosis, and reproduction. Our previous work identified five Halloween genes from Locusta migratoria, but their functions are currently unknown. In this study, the sequences of these five Halloween genes were analyzed and characterized. LmCYP307a2, LmCYP306a1, LmCYP ...
... In applications of chitin, one of the most abundant resources on earth, human milk oligosaccharides with many health functions were synthesized by transglycosylation of β‐N‐acetylhexosaminidase. Synthesis of new transfer products can be expected by other β‐N‐acetylhexosaminidases in nature. A total of 38 microorganisms that secrete β‐N‐acetylhexosaminidases with transglycosylation activity were is ...
... Chitin and its derivatives show ability to form biologically active compounds. They show novel characteristics and broad applications, especially in biomedical field. Chitin can be derived from crustaceans, insects or fungi by chemical method including deproteinization and demineralization by using chemicals. However, as the chemicals may exert adverse effects on the environment, and hence Chitin ...
... Crayfish have become a heavily consumed food and its chitin-rich shell is of great value in terms of waste conversion. This study found a novel chitinase (EaChi40) from a marine bacterium Exiguobacterium antarcticum. The gene was cloned and expressed as a soluble protein of 40 kDa, having optimal activity at pH 6.0 and 30 °C. EaChi40 showed good stability and high specific activity, and kinetic st ...
... To find renewable and sustainable alternatives to reduce the severe environmental impact of single-use synthetic plastic packaging, glucose-crosslinked gelatin films containing different amounts of chitin nanowhiskers (CNWs) were prepared. CNWs were first prepared by acid hydrolysis of chitin from shrimps, and characterized (morphological and thermal properties), before their addition into film-fo ...
biocompatibility; biodegradability; cancer therapy; chitin; chitosan; collagen; drug delivery systems; epithelium; fibroblasts; nanobiotechnology
Abstract:
... Chitin and its derivative chitosan are highly abundant polymers in nature, appearing in both the shells and exoskeletons of various marine and non-marine species. Since they possess favorable properties, such as biocompatibility, biodegradability, non-toxicity, and non-immunogenicity, they have gained recent attention due to their enormous potential biomedical applications. The polycationic surfac ...
... Chitin is the most abundant natural amino polysaccharide, showing various practical applications owing to its functional properties. However, there are barriers in the development due to the difficulty of chitin extraction and purification, regarding its high crystallinity and low solubility. In recent years, some novel technologies such as microbial fermentation, ionic liquid, electrochemical ext ...
... The high molecular weight of chitin, as a biopolymer, challenges its extraction due to its insolubility in the solvents. Also, chitosan, as the N-deacetylated form of chitin, can be employed as a primary material for different industries. The low mechanical stability and poor plasticity of chitosan films, as a result of incompatible interaction between chitosan and the used solvent, have limited i ...
... Nacre mimetic coatings are attractive candidates for food packaging, electronics, textiles, protective insulation, and flame retardant materials. Inspired by the hierarchical structure of nacre, we present an environmentally friendly strategy to construct robust and flame retardant films using chitin, which is an abundantly available biopolymer. Chitin was phosphorylated to make it water-soluble. ...
... The degradation of polymeric chitin by chitinase liberates soluble N-acetyl glucosamine oligosaccharides (GlcNAcₙ≥₂), a source of nutrition that can also induce a state of natural genetic competence in Vibrio parahaemolyticus. This analysis revealed that among seven predicted chitinases, the synergistic action of VPA0055 (ChiA2), VP0619 (ChiB), and VPA0832 (Cdx) were essential for the robust growt ...
... BACKGROUND: The current system in the processing of seafood leads to accumulation of many waste products, such as shells, tails, heads and bones. This seafood waste can be exploited for the extraction of chitin, with numerous applications in different fields. Seafood waste treatment can produce some valuable products. For the valorization of chitin, its degradation is an important step that can be ...
... Traditionally, chitin nanowhiskers (ChNW) have been synthesized using acid or alkali hydrolysis, which is not a fully green and sustainable process. Here, we demonstrate a novel, two-step, environmentally friendly process for producing chitin nanowhiskers with improved yield and colloidal stability. The process begins by swelling the amorphous regions of native chitin in a Natural Deep Eutectics S ...
Nilaparvata lugens; RNA interference; chitin; chitin synthase; egg hatchability; eggs; embryogenesis; genes; insects; pest control; pests; rice; tandem mass spectrometry; transmission electron microscopy
Abstract:
... Many holo‐ and hemimetabolous insects enhance their eggshells during embryogenesis by forming a serosal cuticle (SC). To date, scholarly understanding of the SC composition and SC‐related gene functions has been limited, especially for hemimetabolous insects. In this study, we initially performed transmission electron microscopic (TEM) observation and chitin staining of the SC in Nilaparvata lugen ...
... Fusarium graminearum is one of the primary causal agents of Fusarium head blight (FHB) on wheat and barley. FHB reduces grain yield and contaminates grain with various mycotoxins, including deoxynivalenol (DON). DON acts as a virulence factor to promote the fungus passing the rachis node and spreading throughout the head of wheat but not barley. Reactive oxygen species (ROS) are one of the earlies ...
... The ubiquitous present antibiotics in aquatic environment is attracting increasing concern due to the dual problems of bioaccumulation toxicity and antibiotic resistance. In this study, a low-cost chitin-biocalcium (CC) composite was developed by a facile alkali activation process from shell waste for typical antibiotics ciprofloxacin (CIP) removal. Response surface methodology (RSM) was utilized ...
... Conductive hydrogel flexible sensors have attracted considerable research interest because of their good conductivity, flexibility, and biocompatibility. However, conventional hydrogels suffer from dehydration under ambient environments and freezing at low temperatures. Herein, we prepared a chitin/polyacrylamide organohydrogel with highly stretchable, anti-freezing, and anti-drying properties. Th ...
... The development of adsorbents by using the byproducts or waste from large-scale industrial and agricultural production is of great significance, and is considered to be an economic and efficient strategy to remove the heavy metals from polluted water. In this work, a novel chitin/EM@Fe₃O₄ nanocomposite hydrogel was obtained from a NaOH/urea aqueous system, where the proteins of egg shell membrane ...
... KEY MESSAGE: We show that in rice, the amino acid-conjugates of JA precursor, OPDA, may function as a non-canonical signal for the production of phytoalexins in coordination with the innate chitin signaling. The core oxylipins, jasmonic acid (JA) and JA-Ile, are well-known as potent regulators of plant defense against necrotrophic pathogens and/or herbivores. However, recent studies also suggest t ...
... Chitosan is a naturally occurring biological macromolecule and second most abundant polysaccharide next to cellulose, derived from deacetylation of chitin. Due to its biocompatibility, biodegradability, nontoxic and broad spectrum of antimicrobial activity, it has become an important field of drug delivery system study. With the advancement in nanotechnology, chitosan based nanoformulations have s ...
... Conventionally, the commercial supply of chitin and chitosan relies on shellfish wastes as the extraction sources. However, the fungal sources constitute a valuable option, especially for biomedical and pharmaceutical applications, due to the batch-to-batch unsteady properties of chitin and chitosan from conventional ones. Fungal production of these glycans is not affected by seasonality enables a ...
... The necessity for non-chemical approaches has grown as awareness of the dangers posed by pesticides has spread. Chitosan, due to its biocompatibility, biodegradability, and bioactivity is one the effective choice in phytopathology. Chitosan is a biopolymer that reduces plant diseases through two main mechanisms: (1) Direct antimicrobial function against pathogens, including plasma membrane damage ...
... The agriculture sector is the building block of an economy with more than 60% of the world population depending on it for livelihood. Among the many crops, rice is the most important income source. It is the staple food for more than half of the world population. In spite of its huge demand, rice production has been dwindling due to various constraints. Chitosan nanoparticles (ChNP) are an excelle ...
... Helminth diseases have long been a threat to the health of humans and animals. Roundworms are important organisms for studying parasitic mechanisms, disease transmission and prevention. The study of parasites in the giant panda is of importance for understanding how roundworms adapt to the host. Here, we report a high‐quality chromosome‐scale genome of Baylisascaris schroederi with a genome size o ...
... A major functional division in ectomycorrhizal fungi is between taxa with hydrophobic ectomycorrhizae (strong proteolytic capabilities, deep nitrogen (N) acquisition, and extensive hyphal development) versus hydrophilic ectomycorrhizae (limited hyphal development, shallow N acquisition, and weak proteolytic capabilities). These two groups may differ in dynamic responses to recent plant carbon (C) ...
... Phosphoglucose isomerase (PGI) is a key enzyme that participates in polysaccharide synthesis, which is responsible for the interconversion of glucose‐6‐phosphate (G‐6‐P) and fructose‐6‐phosphate (F‐6‐P), but there is little research focusing on its role in fungi, especially in higher basidiomycetes. The pgi gene was cloned from Lentinula edodes and named lepgi. Then, the lepgi‐silenced strains wer ...
... The Chinese pangolin (Manis pentadactyla) is a critically endangered mammal with a highly specialized diet. To enhance nutritional knowledge of its diet, we analyzed the colony composition of a nest of Polyrhachis dives ants, which is the key natural prey in the Chinese pangolin's diet. In addition, we determined the nutrient composition of the total colony compared with adult ants. Nutrients quan ...
... Chitooligosaccharides (COSs) have a widespread range of biological functions and an incredible potential for various pharmaceutical and agricultural applications. Although several physical, chemical, and biological techniques have been reported for COSs production, it is still a challenge to obtain structurally defined COSs with defined polymerization (DP) and acetylation patterns, which hampers t ...
... The gut microbiome is integral for the host’s living and environmental adaptation and crucially important for understanding host adaptive mechanisms. The red fox (Vulpes vulpes) dominates a wider ecological niche and more complicated habitat than that of the corsac fox (V. corsac). However, the adaptive mechanisms (in particular, the gut microbiome responsible for this kind of difference) are stil ...
... An 8-week feeding trial was performed to compare the supplementation of full-fat black soldier fly, Hermetia illucens, larvae (BSFL) versus pre-pupae (BSFP) in the diets of largemouth bass, Micropterus salmoides, juveniles. Both BSFL and BSFP were produced in the laboratory from the same batch and ingredients although BSFP tended to have higher protein and amino acid content. Four isonitrogenous a ...
... Members of the genus Devosia are known for their abilities to degrade deoxynivalenol (DON). The type strain Devosia beringensis S02ᵀ (= JCM 33772 = CCTCC AB 2019343) was isolated from sediment of the Bering Sea and identified in 2021. However, the genome sequence of D. beringensis S02ᵀ remains unclear, which complicates the exploration into the function and ecological role of this strain in marine ...
... European green crab (Carcinus maenas) is an invasive species globally and is considered marine waste that needs to be eliminated. In this study, the green crab was biorefined via a clean enzymes-assisted bioprocess into multiple value-added bioproducts, i.e., crab oil, minerals, carotenoprotein, and chitin. The recovered crab oil was rich in unsaturated fatty acids such as EPA and DHA as per GC-MS ...
... Chitin is a polymer of N-acetylglucosamine and provides structure and stability to the exoskeleton of crustaceans, insects and fungal cell walls. Intermolecular hydrogen bonds between polymer chains create a very tight crystalline fibre network with excellent mechanical properties. Chitins’ antimicrobial properties and high biocompatibility make it an attractive polymer source for the production o ...
Gleb E. Aktuganov; Violetta R. Safina; Nailya F. Galimzianova; Elena A. Gilvanova; Lyudmila Yu. Kuzmina; Alexander I. Melentiev; Andrei H. Baymiev; Sergey A. Lopatin
... The article proves the ability of the entomopathogenic strain B. thuringiensis var. dendrolimus B-387 to high the constitutive production (3–12.5 U/mL) of extracellular chitosanase, that was found for the first time. The enzyme was purified in 94-fold by ultrafiltration, affinity sorption and cation-exchange chromatography and characterized biochemically. The molecular mass of the chitosanase dete ...
Hericium; cellulose; chitin; crosslinking; gel strength; hydrogels; molecular weight; ultrasonics
Abstract:
... Chitin hydrogels with different molecular weight (Mw) were prepared by the ultrasonic degradation of chitin from Hericium erinaceus residue. The effects of Mw on the properties of the prepared chitin hydrogels were investigated. Results showed that the Mw of chitin was decreasing with the prolongation of the ultrasonic time. As the decrease of Mw, the gel strength of the chitin hydrogels weakened, ...
Alexandro Escobar‐Ortiz; Diego Hernández‐Saavedra; Jaime Lizardi‐Mendoza; Iza Fernanda Pérez‐Ramírez; Ofelia Mora‐Izaguirre; Minerva Ramos‐Gómez; Rosalía Reynoso‐Camacho
... The consumption of cricket (Acheta domesticus, AD) has recently increased due to its high nutritional and bioactive compound contents. However, limited studies have explored the beneficial effects of AD consumption. Therefore, we aimed to evaluate the protective effect of a diet supplemented with AD flour on obesity control and its complications in an in vivo model. The chemical profiling of the A ...
... The genus of Trichoderma are mostly found in soil. Trichoderma species are known as probiotic with biocontrol and biofertilizer activity. They are producers of secondary metabolites like volatile organic compounds (VOCs) with antifungal, antibacterial, and growth promoter properties. Trichoderma VOCs can induce resistance to plant pathogens leading to improved plant growth and health. In this stud ...
... The biodegradability and mechanical properties of polysaccharides are dependent on their architecture (linear or branched) as well as their crystallinity (size of crystals and crystallinity percent). The amount of crystalline zones in the polysaccharide significantly governs their ultimate properties and applications (from packaging to biomedicine). Although synthesis, characterization, and proper ...
... The global decline of bee populations has several factors, including pathogens, which need overcome the insect defenses such as the physical barriers, the body cuticle and peritrophic matrix (primary defenses), as well as the secondary defenses with antimicrobial peptides (AMPs) and the enzyme lysozyme. The regulation of immune defenses according to the infection risks raises questions about the i ...
... Invasive aspergillosis and scedosporiosis are life-threatening fungal infections with similar clinical manifestations in immunocompromised patients. Contrarily, Scedosporium apiospermum is susceptible to some azole derivative but often resistant to amphotericin B. Histopathological examination alone cannot diagnose these two fungal species. Pathogenesis studies could contribute to explore candidat ...
... Insects prevent uncontrolled penetration of water and xenobiotics by producing an impermeable cuticle. The major component of the cuticle is chitin that adopts a crystalline structure thereby contributing to cuticle stability. Our understanding of the contribution of chitin to the cuticle barrier function is limited. Here, we studied the role of the DOMON domain protein Knickkopf (LmKnk) that is i ...
active sites; chitin; chitinase; circular dichroism spectroscopy; computer simulation; endothermy; heat tolerance; temperature
Abstract:
... Biochemical and biophysical studies revealed that chitinase O from Chitiniphilus shinanonensis (CsChiO) exhibits considerable thermotolerance, possibly due to the formation of a stable structural conformation. CsChiO is an exochitinase with a temperature optimum of 70 °C. The secondary structures of CsChiO and its catalytic domain (Cat-CsChiO) are only marginally affected upon heating up to 90 °C, ...
cellulose; chitin; community structure; denitrification; land degradation; meadows; metagenomics; microbial communities; microbiome; nitrification; nitrogen; risk; soil; soil microorganisms; soil nutrients; soil organic carbon; structural equation modeling; sugars; total nitrogen; variance; China
Abstract:
... Grassland degradation has become a serious environmental problem worldwide. However, the link between grassland degradation and soil functional microorganisms remains unclear. Here, we investigated the effects of subalpine meadow degradation on the taxonomic and functional communities of the soil microbiome on Mount Wutai on the eastern margin of the Loess Plateau, China, using shotgun metagenomic ...
... Developing fully biobased products with functionality in a green fashion is highly desirable to meet the increasing demand for environmental sustainability and mitigate “white pollution” by petroleum-based counterparts. Here, chitin from shrimp shells was propionylated to obtain chitin propionate (CP) with significantly improved solubility in organic solvents, organosolv lignin (OSL) was extracted ...
... To maintain the freshness of fruits and to meet environmental and consumer needs, a biobased packaging film with long-lasting antimicrobial activity was developed in this article. Liquefied ball-milled shrimp shell chitin/polyvinyl alcohol (LBSC/PVA) blend films containing varying concentrations (0, 1, 2, 3, 4, 5 wt%) of the β-cyclodextrin/cinnamaldehyde (β-CD/CA) inclusion were prepared and chara ...
Helicoverpa armigera; RNA; chitin; chitinase; food chemistry; growth and development; mechanism of action
Abstract:
... In a previous study, we found that compound ZQ-8 had a strong impact on the growth and development of Helicoverpa armigera. However, the mechanism and target of ZQ-8 are not clear, which makes it difficult to optimize the structure of this compound. In this study, the preliminary mode of action of ZQ-8 was studied through RNA sequencing and molecular docking. We also analyzed the underlying mechan ...
... The recently proposed order Candidatus Thermoprofundales, currently containing only one family‐level lineage Marine Benthic Group‐D (MBG‐D), is distributed in global subsurface ecosystems and ecologically important, but its diversity, evolution and metabolism remain largely unknown. Here we described two novel family‐level specialized lineages in Ca. Thermoprofundales, JdFR‐43 and HyVt, which are ...
... Some studies have reported the method for treating the spent mushroom substrate (SMS). However, the effective use as a functional raw material based on properties of SMS remains a formidable challenge. In this study, we investigated the usefulness of SMS in agriculture to develop a new method for treating and utilizing it. First, we attempted to isolate chitin/cellulose nanofiber complex (CCNFC) f ...
adhesion; bone formation; bone marrow; chitin; chitosan; crosslinking; extracellular matrix; hydrogels; liquid crystals; mineralization; stress relaxation; ultrasonic treatment; viscoelasticity
Abstract:
... The liquid crystal properties and viscoelasticity of the natural bone extracellular matrix (ECM) play a decisive role in guiding cell behavior, conducting cell signals, and regulating mineralization. Here, we develop a facile approach for preparing a novel polysaccharide hydrogel with liquid crystal properties and viscoelasticity similar to those of natural bone ECM. First, a series of chitin whis ...
... Bombyx mori has been extensively studied but the gene expression control of its embryonic development is unclear. In this study, we performed transcriptome profiling of six stages of B. mori embryonic development using RNA sequencing (RNA‐seq). A total of 12 894 transcripts were obtained from the embryos. Of these, 12 456 transcripts were shared among the six stages, namely, fertilized egg, blasto ...
... E74 is a key transcription factor induced by 20E, which plays a broad role in many physiological events during insect growth and development, including vitellogenesis, organ remodeling and new tissue formation, programmed cell death and metamorphosis. However, whether it is involved in regulating insect chitin biosynthesis remains largely unclear. Here, the E74 gene was identified for the first ti ...
Russia; antimicrobial properties; chitin; chitosan; microbiology; toxicity; veterinary medicine
Abstract:
... Currently biostimulants based on chitosan and its derivatives are increasingly used in veterinary medicine. Chitosan and its modifications have many properties that allow them to be used in beekeeping: natural origin and lack of toxicity, immunomodulatory properties, antimicrobial activity, and the ability to increase resistance and remove heavy metals and toxins from bees. Chitosan is a chitin de ...
... In this study, the physicochemical, rheological, and functional properties of colloidal chitin prepared from commercial shrimp shell α-chitin by acid hydrolysis at ambient temperature for various treatment durations were investigated. With increasing treatment duration, the functional properties viz solubility, water and fat binding capacity, and emulsion capacity increased significantly (p<0.05). ...
... Zataria multiflora Boiss. (ZM) is an aromatic herb that is used in various industries, especially in pharmaceuticals and food. However, the inducer effect of elicitors on ZM, with the aim of increasing the bioactive compounds production, is understudied. This study analyzes the influence of a biotic (chitin) and abiotic (copper nanoparticle) elicitor on ZM under in vitro and greenhouse conditions. ...
... In this study, the effect of pretreatment with three organic solvent systems on enzymatic hydrolysis of chitin was investigated. Firstly, genes coding three chitinolytic enzymes CmChi1, CmChi4, and CmNAGase from Chitinolyticbacter meiyuanensis SYBC-H1 were cloned and functionally expressed. Then, a cocktail capable of producing GlcNAc from chitin was formulated with 71.4% recombinant CmChi1, 23.8% ...
... Edible insects are an important source of proteins, fat, and chitin, which need to be extracted to develop tailored products with a controlled composition. Pulsed electric fields (PEF) is a non-thermal technology that can enhance the extraction. This study explores the effect of PEF on the extraction of protein, fat and chitin from cricket flour, as well as the material's functional properties. Ho ...
... In this study, we assessed the impact of zinc oxide (ZnO) and iron oxide (FeO) (<36 nm) nanoparticles (NPs) as well as their sulphate salt (bulk) counterpart (0, 25, 100 mg/kg) on rice growth and seed quality as well as the microbial community in the rhizosphere environment of rice. During the rice growing season 2021–22, all experiments were conducted in a greenhouse (temperature: day 30 °C; nigh ...
... This study proposed a feasible route for effectively inhibiting degradation of chitin by introducing a novel low-viscosity gluconic acid-based deep eutectic solvents (DESs) aqueous solution. Under the optimal extraction conditions, a high-quality chitin with molecular weight of 3.75 × 10⁵ Da, purity of 94.5 % and yield of 79.1 % was obtained from crab shell. The maximum yield and molecular weight ...
... The insecticidal potential of diatomaceous earth (DE), two chitin synthesis inhibitors [chlorfluazuron (CH) and hexaflumuron (HE)] and their binary mixtures was tested against Callosobruchus maculatus (F.) and C. chinensis (L.) on cowpea seeds. Diatomaceous earth was assessed at rates of 100, 500 and 1000 mg/kg. The two insecticides (CH and HE) were evaluated at rates of 0.5, 1.0 and 2.0 mg/kg. Th ...
... Extraction of chitin from crustacean waste with acidic natural deep eutectic solvents (NADESs) is usually accompanied by degradation of chitin, which lowers the yield and molecular weight of product. Herein, this study proposed a eco-friendly and feasible route for effectively improving the yield and molecular weight of chitin by introducing N-acetyl-D-glucosamine into ternary NADESs. A high molec ...
... OBJECTIVES: Shellfish waste is a primary source for making N-acetyl-D-glucosamine. Thus, establishing a high-efficiency and low-cost bioconversion method to produce N-acetyl-D-glucosamine directly from shellfish waste was promising. RESULTS: A mutant C81 was obtained from Chitinolyticbacter meiyuanensis SYBC-H1 via ⁶⁰Co-γ irradiation. This mutant C81 showed the highest chitinase activity of 9.8 U/ ...
acid hydrolysis; chemical composition; chitin; cost effectiveness; desorption; ethanol; nuclear magnetic resonance spectroscopy; phosphoric acid; polymerization
Abstract:
... A method of producing oligomeric chitin using sonication-assisted phosphoric acid hydrolysis was introduced. The processing was continuous and scalable. Oligomeric chitin fractions with narrow distributions of degree of polymerization were obtained by differential precipitation using ethanol as precipitating agent at different ethanol-to-phosphoric-acid-solution volume ratios. The yield of oligome ...
... Biopolymer chitin and its derivatives have a wide range of applications in different fields. Chitin is primarily produced from marine crustacean by strong acid and strong alkali treatment. To reduce the environmental hazardous in the production of chitin, post-fermentation fungal biomass waste like fungi cell walls provides a viable alternative source for chitin. In this study, improved chitin con ...
... It is hard to degrade untreated highly crystalline chitin. In this study, two solvents pretreatment chitin (acid swollen chitin (AC), super fine chitin (FC)) and microwave-heating method were used to enhance nonspecific enzymatic hydrolysis (lysozyme and pepsin), which obviously improved the enzymolysis rates by at least 1.31 times. Characterizations of chitin substrates (Mᵥ, SEM, XRD) showed that ...
... Streptomyces chilikensis RC1830 was previously isolated as a novel chitinolytic streptomycete from Chilika Lake, Odisha, India. The strain RC1830 is a representative member of the soil-dwelling, filamentous Streptomyces group that produces the majority of natural antibiotics and secondary metabolites. The objective of this work was to assess the chitin degradation ability and whole-genome sequence ...
... As a natural polymer, chitin has excellent biological properties such as biodegradability and immunological, antibacterial, and wound-healing activities and has numerous applications in cosmetics, drug delivery, and pharmaceuticals. Organic polymer monoliths have also drawn significant attention, owing to their high permeability, large surface area, and high mechanical strength. They are usually a ...
... Paper is an inherent hydrophilic material due to the hydroxyl groups contained in cellulose, which limits its applications in packaging materials. This study proposed a simple one-step method for preparing hydrophobic paper by introduction of chitin nanocrystals (ChNCs) and hexadecyltrimethoxysilane (HDTMS). The surface chemistry and morphology of coated paper were studied in detail. The 2%ChNCs/H ...
... Chitin, a kind of polysaccharide mainly obtained from food waste, has emerged as an important biodegradable biopolymer in composite materials. The difficulty of aldehyde modification, which greatly limited the application of chitin nanocrystals, was addressed by applying a facile route of partial deacetylation followed by periodate oxidation in this study. Deacetylation occurred on the surface of ...
... Chitin, the major structural polysaccharide in arthropods such as insects and mites, is a linear polymer of N-acetylglucosamine units. The growth and development of insects are intimately coupled with chitin biosynthesis. The membrane-bound β-glycosyltransferase chitin synthase is known to catalyze the key polymerization step of N-acetylglucosamine. However, the additional proteins that might assi ...
... Insect biomass obtained from large-scale mass-rearing of insect larvae has gained considerable attention in recent years as an alternative and sustainable source of food and feed. A byproduct from mass-rearing of insect larvae is the shed cuticles – the most external components of insects which are a relevant source of the polysaccharide chitin. While it has been shown that chitin modulates the gu ...