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You searched for: Subject term cryopreservation Remove constraint Subject term: cryopreservation Subject term Brazil Remove constraint Subject term: Brazil
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1. Indicators of conservation priorities for medicinal plants from seasonal dry forests of northeastern Brazil

Author:
Juliana Loureiro Almeida Campos; Ulysses Paulino Albuquerque
Source:
Ecological indicators 2021 v.121 pp. 106993
ISSN:
1470-160X
Subject:
Anacardium occidentale; Copaifera langsdorffii; Handroanthus impetiginosus; caatinga; cryopreservation; demographic statistics; ecosystems; indigenous species; manufacturing; pharmaceutical industry; reforestation; systematic review; Brazil
Abstract:
... Medicinal plants are used by local populations to treat numerous diseases, and many species are used as raw materials for the manufacture of medicines sold by the pharmaceutical industry. However, the intensive collection of these resources poses great challenges related to their conservation. Through a systematic review, we gathered information on the versatility of medicinal trees from seasonal ...
DOI:
10.1016/j.ecolind.2020.106993

2. Status of the Ex Situ and In Situ Conservation of Brazilian Crop Wild Relatives of Rice, Potato, Sweet Potato, and Finger Millet: Filling the Gaps of Germplasm Collections

Author:
Marcelo B. Medeiros; José F. M. Valls; Aluana G. Abreu; Gustavo Heiden; Suelma Ribeiro-Silva; Solange C. B. R. José; Izulmé R. I. Santos; Alexandre M. A. Passos; Marília L. Burle
Source:
Agronomy 2021 v.11 no.4 pp. -
ISSN:
2073-4395
Subject:
Eleusine coracana; Eleusine indica; Solanum chacoense; Solanum commersonii; Tristachya; agronomy; climate change; cryopreservation; dormancy; ex situ conservation; genes; germplasm; germplasm conservation; potatoes; rice; seed germination; sweet potatoes; temperature; viability; Brazil
Abstract:
... This study presents the status of ex situ and in situ conservation for the crop wild relatives of rice, potato, sweet potato, and finger millet in Brazil, and the subsequent germplasm collection expeditions. This research is part of a global initiative entitled “Adapting Agriculture to Climate Change: Collecting, Protecting, and Preparing Crop Wild Relatives” supported by the Global Crop Diversity ...
DOI:
10.3390/agronomy11040638

3. 38 Ram sperm longevity after cryopreservation in extender containing L-carnitine

Author:
C. Souza; F. Brandão; J. Santos; V. Alfradique; V. Brair; L. Prellwitz; P. S. Rangel; A. Silva; J. M. Souza-Fabjan
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 145
ISSN:
1031-3613
Subject:
acrosome reaction; albumins; breeding season; carbon dioxide; carnitine; cooling; cryopreservation; freeze-thaw cycles; freezing; funding; head; lactic acid; lipid peroxidation; plasma membrane; protective effect; pyruvic acid; rams; semen; semen extenders; spermatozoa; straw; Brazil
Abstract:
... The cryopreservation process causes oxidative stress to the sperm cell, and the addition of antioxidants to the extender for semen freezing helps sperm protection. This study assessed the effect of L-carnitine (LC) concentrations (0, 5, or 10mM LC) on two ram semen extenders (Tris-egg yolk or the commercial optiXcell IMV medium (IMV Technologies)) for semen cryopreservation. Four Santa Inês rams w ...
DOI:
10.1071/RDv32n2Ab38

4. Endophytic fungi of Brunfelsia uniflora: isolation, cryopreservation, and determination of enzymatic and antioxidant activity

Author:
Sara Jane Marsola; Lais Freitas Jorge; Adriano Borges Meniqueti; Miria Benetati Delgado Bertéli; Thaís Emanuelle Feijó de Lima; José Luiz Bezerra; Ana Daniela Lopes; Zilda Cristiani Gazim; Juliana Silveira do Valle; Nelson Barros Colauto; Giani Andrea Linde
Source:
World journal of microbiology & biotechnology 2022 v.38 no.6 pp. 94
ISSN:
0959-3993
Subject:
Ascomycota; Brunfelsia; antioxidant activity; antioxidants; biomass; biotechnology; carboxymethylcellulose; chemical constituents of plants; cryopreservation; emetics; endo-1,4-beta-glucanase; endophytes; fungi; laccase; malt extract; mycelium; oxidation; sucrose; viability; wheat; Brazil
Abstract:
... Brunfelsia uniflora (Pohl.) D. Don (Solanaceae), commonly known as manacá-de-cheiro, is widely distributed in Brazil and used by local indigenous peoples as an antirheumatic, antisyphilitic, depurative, emetic, vermifuge, and purgative agent. Several studies have examined the biological activities and phytochemical profile of Brunfelsia; however, few have focused on the diversity of endophytic mic ...
DOI:
10.1007/s11274-022-03278-5

5. Establishment and multiapproach characterization of Amblyomma sculptum (Acari: Ixodidae) cell line (ASE-14) from Brazil

Author:
Leidiane Lima-Duarte; Ana Carolina Castro-Santiago; Jaqueline Valéria Camargo; Ana Beatriz Stein Machado Ferretti; Luís Adriano Anholeto; Melissa Carolina Pereira; Priscila Ikeda; Carlos Alberto Perez; Gustavo Seron Sanches; Maria Izabel Camargo-Mathias; Diogo Cavalcanti Cabral-de-Mello; Rosangela Zacarias Machado; Marcos Rogério André; Darci Moraes Barros-Battesti
Source:
Ticks and tick-borne diseases 2022 v.13 no.4 pp. 101951
ISSN:
1877-959X
Subject:
Amblyomma sculptum; Anaplasma marginale; Babesia; Bartonella; Coxiella; Ehrlichia canis; Mycoplasma; Rickettsia; Theileria; adults; bacteriology; cell lines; chromosomes; cryopreservation; cytogenetic analysis; diploidy; genes; mitochondria; phase-contrast microscopy; polysaccharides; ticks; virology; Brazil
Abstract:
... The establishment and characterization of the ASE-14 cell line derived from embryos of Amblyomma sculptum is described here. Primary cultures were started, and after 60 days of culturing a confluent monolayer was formed and the first subculture was then carried out. After this, new subcultures were carried out every 4 weeks. Cryopreservation of cells was successful only after the 14ᵗʰ subculture. ...
DOI:
10.1016/j.ttbdis.2022.101951

6. A systematic review and meta-analysis of spermatozoa cryopreservation, in vitro and in vivo fertility practices in water buffalo

Author:
Syed Aftab Hussain Shah; Syed Murtaza Hassan Andrabi
Source:
Veterinary research communications 2021 v.45 no.2-3 pp. 47-74
ISSN:
0165-7380
Subject:
Philippines; blastocyst; buffaloes; cryopreservation; genetic improvement; meta-analysis; metadata; semen; semen extenders; systematic review; veterinary medicine; world wide web; Brazil; Bulgaria; China; Egypt; India; Indonesia; Iran; Italy; Pakistan; Saudi Arabia; Thailand; Vietnam
Abstract:
... We explored different aspects of buffalo spermatozoa during cryopreservation. The meta-data comprised of 285 studies, published from January 2008 to March 2020. A free web tool CADIMA as well as PRISMA 2009 Flow Diagram were used for carrying out this study. The inter-reviewer agreement among studies allocated was satisfactory for criteria A (selection bias), B (performance bias), C (detection bia ...
DOI:
10.1007/s11259-021-09789-0

7. Amides as cryoprotectants for the freezing of Brycon orbignyanus sperm

Author:
Carolina Trindade Perry; Carine Dahl Corcini; Andreia Nobre Anciuti; Marina Vianna Otte; Sara Lorandi Soares; Juan Ramon Esquivel Garcia; Juan Ramon Esquivel Muelbet; Antonio Sergio Varela
Source:
Aquaculture 2019 v.508 pp. 90-97
ISSN:
0044-8486
Subject:
Brycon orbignyanus; DNA fragmentation; aquaculture; cryopreservation; cryoprotectants; dimethylformamide; endangered species; fish; flow cytometry; freezing; lipid peroxidation; liquids; membrane fluidity; mitochondria; nitrogen; plasma membrane; reactive oxygen species; sperm motility; spermatozoa; straw; thawing; vapors; Brazil
Abstract:
... The objective of this study was to evaluate the use of different amides as an alternative for the cryopreservation of sperm from the endangered species, Bryconorbignyamus, an important fish for aquaculture in Brazil. The cryoprotectants which were combined with Beltsville Thawing Solution™ extender, each at the concentrations of 2.5%, 5%, 7.5%, and 10%, were as follows: (1) dimethylacetamide (DMA) ...
DOI:
10.1016/j.aquaculture.2019.03.015

8. Cattle breed affects in vitro embryo production in a large-scale commercial program on dairy farms

Author:
Izabelle Pereira de Lacerda; Margot Alves Nunes Dode; Marcelo Machado Souza Lima; Breno Fonseca Guerra; Eduardo Silva Costa; Gisele Rodrigues Moreira; José de Oliveira Carvalho
Source:
Livestock science 2020 v.240 pp. 104135
ISSN:
1871-1413
Subject:
Holstein; blastocyst; bulls; crossbreds; cryopreservation; oocytes; pregnancy rate; semen; spermatozoa; Brazil
Abstract:
... Sexed cryopreserved semen is widely used in vitro embryo production (IVEP) for cattle reproduction in Brazil. However, differences between donor breeds regarding variables related to the IVEP process have been reported. This study aimed to evaluate factors affecting the efficiency of IVEP using donors of different genotypes and sexed sperm from Gir and Holstein bulls in a commercial program of IVE ...
DOI:
10.1016/j.livsci.2020.104135

9. Rickettsia rickettsii (Rickettsiales: Rickettsiaceae) Infecting Amblyomma sculptum (Acari: Ixodidae) Ticks and Capybaras in a Brazilian Spotted Fever-Endemic Area of Brazil

Author:
Francisco B Costa; Monize Gerardi; Lina de C Binder; Hector R Benatti; Maria Carolina de Azevedo Serpa; Beatriz Lopes; Hermes R Luz; Katia M P M B Ferraz; Marcelo B Labruna; Kevin Macaluso
Source:
Journal of medical entomology 2020 v.57 no.1 pp. 308-311
ISSN:
1938-2928
Subject:
Amblyomma sculptum; DNA; Hydrochoerus hydrochaeris; Rickettsia rickettsii; Rocky Mountain spotted fever; antigens; bacteria; blood serum; cryopreservation; fever; fluorescent antibody technique; guinea pigs; hosts; lungs; spleen; ticks; Brazil
Abstract:
... The bacterium Rickettsia rickettsii is the agent of Brazilian spotted fever (BSF), a highly fatal disease that is transmitted in Brazil mainly by the tick Amblyomma sculptum Berlese, which uses capybaras (Hydrochoerus hydrochaeris Linnaeus) as major hosts. In 2015, we captured nine capybaras in a BSF-endemic area of southeastern Brazil. From each capybara, we collected blood sera that were tested ...
DOI:
10.1093/jme/tjz141

10. Successful cryopreservation of Vitis vinifera 'Chardonnay' from both in vitro and growth chamber source plants

Author:
J. C. Bettoni; R. Bonnart; A. Shepherd; A. A. Kretzschmar; G. M. Volk
Source:
Acta horticulturae 2019 v. no.1234 pp. 211-218
ISSN:
0567-7572
Subject:
Vitis vinifera; ascorbic acid; crops; cryopreservation; cultivars; exposure duration; gene banks; glutathione; growth chambers; nitrogen; regrowth; salicylic acid; shoot tips; sucrose; tissue culture; Brazil; United States
Abstract:
... Both the United States and Brazil maintain genebank collections of vegetatively propagated crops, including grapevine (Vitis). Vitis collections are expensive to maintain in the field and are vulnerable to abiotic and biotic threats. When robust methods are available, cryopreservation provides an opportunity to securely back-up collections with lower maintenance costs than those of duplicated fiel ...
DOI:
10.17660/ActaHortic.2019.1234.28

11. Cryopreservation: Extending the viability of biological material from sea urchin (Echinometra lucunter) in ecotoxicity tests

Author:
Raphaela Cantarino Ribeiro; Alexandra Caroline da Silva Veronez; Thaís Tristão Tovar; Serean Adams; Dayse Aline Bartolomeu; Clayton Peronico; Tatiana Heid Furley
Source:
Cryobiology 2018 v.80 pp. 139-143
ISSN:
0011-2240
Subject:
Echinometra lucunter; animals; cryopreservation; cryoprotectants; ecotoxicology; eggs; larvae; nitrogen; spawning; spermatozoa; sucrose; temperature; vapors; viability; weather; Brazil
Abstract:
... The sea urchin, Echinometra lucunter, is widely used in embryo-larval tests for ecotoxicological studies in Brazil and other countries. For each test, sea urchins are collected from the wild and this can cause impact on wild populations and it is limited by the weather and season which in turn limits the ability to carry out the tests. Cryopreservation is a method of live biological material stora ...
DOI:
10.1016/j.cryobiol.2017.10.002

12. First steps towards Echinometra lucunter embryo cryopreservation

Author:
Marcella Bueno Ribeiro; Tatiana Furley; Flávia Regina Spago; Estefania Paredes
Source:
Cryobiology 2018 v.80 pp. 51-54
ISSN:
0011-2240
Subject:
Echinometra lucunter; bioassays; cryopreservation; dimethyl sulfoxide; larvae; oocytes; propylene glycol; toxic substances; toxicity; Brazil
Abstract:
... We have studied the sensitivity to cryoprotecting agents of different embryos of the local sea urchin, Echinometra lucunter which is the species used for embryo-larval bioassays in Brazil. We have located significant differences between both species sensitivity to cryoprotecting agents; while for P. lividus propylene glycol was the less toxic compound for most development stages, whereas for E. lu ...
DOI:
10.1016/j.cryobiol.2017.12.005

13. Melatonin Added to Cryopreservation Extenders Improves the Mitochondrial Membrane Potential of Postthawed Equine Sperm

Author:
Renata Lançoni; Eneiva Carla Carvalho Celeghini; Maíra Bianchi Rodrigues Alves; Kleber Menegon Lemes; Angela Maria Gonella-Diaza; Leticia Zoccolaro Oliveira; Rubens Paes de Arruda
Source:
Journal of equine veterinary science 2018 v.69 pp. 78-83
ISSN:
0737-0806
Subject:
acrosome; antioxidant activity; cryopreservation; ferulic acid; fluorescent dyes; freezing; linear models; melatonin; membrane potential; mitochondria; mitochondrial membrane; plasma membrane; reactive oxygen species; semen; sperm quality; stallions; Brazil
Abstract:
... Reactive oxygen species (ROS) can induce sperm damage, especially after cryopreservation. Melatonin (MEL) is a potent amphiphilic antioxidant, allowing free penetration to any compartment of cell. Ferulic acid (FA) is a phenolic compound exhibiting a wide range of beneficial effects against several pathologies due to its antioxidant property. This study aimed to evaluate the effect of MEL and FA o ...
DOI:
10.1016/j.jevs.2018.06.006

14. Sodium caseinate improves longevity and fertility of frozen bull semen

Author:
Jefferson Viana Alves Diniz; Rafael Augusto Satrapa; Lorenzo Garrido T.M. Segabinazzi; João Alexandre Matos Carneiro; Eunice Oba; Federico Ozanam Papa; Camila de Paula Freitas Dell’Aqua; Bárbara Loureiro; José Antonio Dell’Aqua Junior
Source:
Theriogenology 2020 v.154 pp. 59-65
ISSN:
0093-691X
Subject:
artificial insemination; blastocyst; cryopreservation; egg yolk; flow cytometry; freeze-thaw cycles; head; longevity; mitochondria; plasma membrane; semen; sodium caseinate; spermatozoa; Brazil
Abstract:
... This study aimed to evaluate the effect of sodium caseinate added into freezing extender on the sperm parameters of cryopreserved bull semen and in vitro and in vivo fertility. One ejaculate of 30 bulls was used and processed using Botu-Bov (Botupharma, Botucatu, Brazil) with the addition of 20% egg yolk (EY) or 15% egg yolk with 2% sodium caseinate (EY + SC), subsequently submitted to freezing. S ...
DOI:
10.1016/j.theriogenology.2020.05.028

15. Additional information on Meloidogyne polycephannulata and its proposal as a junior synonym of M. incognita

Author:
Jessica M.S. Monteiro; Vanessa S. Mattos; Marcilene F.A. Santos; Ana C.M.M. Gomes; Valdir R. Correa; Daniela A. Sousa; Juvenil E. Cares; Jadir B. Pinheiro; Regina M.D.G. Carneiro
Source:
Nematology v.21 no.2 pp. 129-146
ISSN:
1388-5545
Subject:
Meloidogyne incognita; amplified fragment length polymorphism; carrots; cryopreservation; diagnostic techniques; esterases; host range; internal transcribed spacers; mitochondria; morphometry; phenotype; phylogeny; random amplified polymorphic DNA technique; redescriptions; ribosomal RNA; root-knot nematodes; Brazil
Abstract:
... The type population of Meloidogyne polycephannulata is synonymised with M. incognita based on morphological and morphometric characters, as well as biochemical, molecular and phylogenetic studies. Morphological variability and a wide host range were reported for M. incognita during its first description and later re-description. Meloidogyne polycephannulata was described in Brazil from specimens c ...
DOI:
10.1163/15685411-00003202

16. Compatible solutes improve regrowth, ameliorate enzymatic antioxidant systems, and reduce lipid peroxidation of cryopreserved Hancornia speciosa Gomes lateral buds

Author:
Débora de Oliveira Prudente; Renato Paiva; Fernanda Carlota Nery; Patrícia Duarte de Oliveira Paiva; José Donizeti Alves; Wesley Pires Flausino Máximo; Luciano Coutinho Silva
Source:
In vitro cellular & developmental biology 2017 v.53 no.4 pp. 352-362
ISSN:
1054-5476
Subject:
ascorbate peroxidase; betaine; buds; catalase; cryopreservation; enzyme activity; hydrogen peroxide; in vitro studies; lipid peroxidation; oxidative stress; proline; regrowth; solutes; storage; superoxide dismutase; Brazil
Abstract:
... Hancornia speciosa is a native fruit species from Brazil with important economic applications. However, the use of this species has been challenged by difficulties in its storage. Cryopreservation is a safe and efficient tool for long-term storage. Nevertheless, cryopreservation may promote reactive oxygen species (ROS) formation with subsequent lipid peroxidation, which causes membrane damage. Th ...
DOI:
10.1007/s11627-017-9830-9

17. Isolation and characterization of a germ cell marker in teleost fish Colossoma macropomum

Author:
Ana Carina Nogueira Vasconcelos; Danilo Pedro Streit; Anna Octavera; Misako Miwa; Naoki Kabeya; Raycon Roberto Freitas Garcia; Daniel Antonio Rotili; Goro Yoshizaki
Source:
Gene 2019 v.683 pp. 54-60
ISSN:
0378-1119
Subject:
Colossoma macropomum; DEAD-box RNA helicases; amino acids; complementary DNA; cryopreservation; evolution; fish; gene expression; genes; genetic markers; genetic variation; gonads; in situ hybridization; indigenous species; messenger RNA; oocytes; open reading frames; spermatogonia; Brazil
Abstract:
... The native Amazonian fish tambaqui (Colossoma macropomum) is the second-largest scaled fish in South America and the most common native species in Brazil. To preserve genetic resources with sufficient genetic diversity through germ cell cryopreservation and transplantation techniques, a molecular marker for identifying the cells is required to trace them during the manipulation processes. The vasa ...
DOI:
10.1016/j.gene.2018.10.027

18. Sperm characterization of the endangered Amazonian fish Hypancistrus zebra: Basic knowledge for reproduction and conservation strategies

Author:
Jôsie S. Caldas; Leandro Godoy
Source:
Animal reproduction science 2019 v.204 pp. 117-124
ISSN:
0378-4320
Subject:
Hypancistrus zebra; assisted reproductive technologies; cryopreservation; flagellum; freshwater fish; germplasm; habitats; ornamental fish; rivers; sperm quality; vigor; Amazonia; Brazil
Abstract:
... Hypancistrus zebra is an ornamental fish endemic to the Xingu River (Brazilian Amazon) and is critically endangered by the construction of a hydroelectric plant in its habitat and illegal fishing. In an attempt to create a germplasm bank for conservation purposes, in the present study there was characterization of H. zebra sperm for the first time and assessment of sperm quality throughout the yea ...
DOI:
10.1016/j.anireprosci.2019.03.012

19. A Comparison of Pyrogen Detection Tests in the Quality Control of Meningococcal Conjugate Vaccines: The Applicability of the Monocyte Activation Test

Author:
Vitor Fernandes Silva; Daniel da Silva Guedes Junior; Ivna Alana da Silveira; Alessandra Santos Almeida; Fernando de Paiva Conte; Isabella Fernandes Delgado; Cristiane Caldeira Silva; Octavio Augusto França Presgrave; Katherine Antunes de Mattos
Source:
ATLA. Alternatives to laboratory animals v.46 no.5 pp. 255-272
ISSN:
0261-1929
Subject:
Limulus; adjuvants; cryopreservation; endotoxins; humans; laboratories; lipopolysaccharides; lipoteichoic acids; models; monocytes; quality control; vaccines; zymosan; Brazil
Abstract:
... The meningococcal C conjugate vaccine (MenCC) is an interesting model with which to test the efficacy of the Monocyte Activation Test (MAT) as an alternative method of pyrogen testing in the quality control of vaccines. The MenCC that has been produced by Bio-Manguinhos in Brazil is in the final development stage, and, as recommended in the guidelines for MenCC production, its pyrogen content must ...
DOI:
10.1177/026119291804600506

20. Applicability of the Monocyte Activation Test (MAT) in the Quality Control of the 17DD Yellow Fever Vaccine

Author:
Katherine Antunes de Mattos; Elaine Cristina Azevedo Navega; Vitor Fernandes Silva; Alessandra Santos Almeida; Cristiane Caldeira da Silva; Octavio Augusto França Presgrave; Daniel da Silva Guedes Junior; Isabella Fernandes Delgado
Source:
ATLA. Alternatives to laboratory animals v.46 no.1 pp. 23-37
ISSN:
0261-1929
Subject:
cryopreservation; endotoxins; industry; interleukin-6; laboratories; lipopolysaccharides; lipoteichoic acids; live vaccines; monocytes; ovalbumin; quality control; quantitative analysis; viruses; yellow fever; Brazil
Abstract:
... The need for alternatives to animal use in pyrogen testing has been driven by the Three Rs concept. This has resulted in the inclusion of the monocyte activation test (MAT) in the European Pharmacopoeia, 2010. However, some technical and regulatory obstacles must be overcome to ensure the effective implementation of the MAT by the industry, especially for the testing of biological products. The ye ...
DOI:
10.1177/026119291804600107

21. Cooling and freezing of sperm from captive, free-living and endangered squirrel monkey species

Author:
Karol G. Oliveira; Regiane R. Santos; Danuza L. Leão; Adriel B. Brito; Julianne S. Lima; Wlaísa V. Sampaio; Sheyla F.S. Domingues
Source:
Cryobiology 2016 v.72 no.3 pp. 283-289
ISSN:
0011-2240
Subject:
Saimiri; captive animals; cooling; cryopreservation; egg yolk; floodplains; freezing; plasma membrane; semen; sperm motility; spermatozoa; sustainable development; thawing; translation (genetics); tropics; Brazil
Abstract:
... Germoplasm banking is an important tool for the preservation of genetic material from Neotropical primates in captivity, and from free living species, especially the endangered ones like Saimiri vanzolinii (Black-headed squirrel monkey), a primate with a low incidence area (870 km2 of floodplains) in the southern part of the Mamirauá Sustainable Development Reserve, Brazil. Therefore, in the prese ...
DOI:
10.1016/j.cryobiol.2016.03.004

22. Cryobanking of aquatic species

Author:
Sonia Martínez-Páramo; Ákos Horváth; Catherine Labbé; Tiantian Zhang; Vanesa Robles; Paz Herráez; Marc Suquet; Serean Adams; Ana Viveiros; Terrence R. Tiersch; Elsa Cabrita
Source:
Aquaculture 2017 v.472 pp. 156-177
ISSN:
0044-8486
Subject:
aquaculture; breeding stock; cryopreservation; freshwater; genetic improvement; genome; germplasm; invertebrates; larvae; marine fish; oocytes; somatic cells; spermatogonia; spermatozoa; Australia; Brazil; Europe; New Zealand; United States
Abstract:
... This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocyte ...
DOI:
10.1016/j.aquaculture.2016.05.042
PubMed:
29276317
PubMed Central:
PMC5737826

23. Histological characterization of Passiflora pohlii Mast. root tips cryopreserved using the V-Cryo-plate technique

Author:
Mariela J. Simão; Myriam Collin; Renata O. Garcia; Elisabeth Mansur; Georgia Pacheco; Florent Engelmann
Source:
Protoplasma 2018 v.255 no.3 pp. 741-750
ISSN:
0033-183X
Subject:
Passiflora; adhesion; adventitious roots; antioxidant activity; cost effectiveness; cryopreservation; explants; histology; indigenous species; osmotic treatment; plant germplasm; plasmolysis; protocols; root tips; saponins; vitrification; Brazil
Abstract:
... Cryopreservation stands out as the main strategy to ensure safe and cost efficient long-term conservation of plant germplasm, especially for biotechnological materials. However, the injuries associated with the procedure may result in structural damage and low recovery rates after cooling. Histological analysis provides useful information on the effects of osmotic dehydration, LN exposure, and rec ...
DOI:
10.1007/s00709-017-1182-0

24. Infection of laboratory colonies of Anopheles mosquitoes with Plasmodium vivax from cryopreserved clinical isolates

Author:
Kathryn Shaw-Saliba; David Clarke; Jorge M. Santos; Maria José Menezes; Caeul Lim; Anjali Mascarenhas; Laura Chery; Edwin Gomes; Sandra March; Sangeeta N. Bhatia; Pradipsinh K. Rathod; Marcelo U. Ferreira; Flaminia Catteruccia; Manoj T. Duraisingh
Source:
International journal for parasitology 2016 v.46 no.11 pp. 679-683
ISSN:
0020-7519
Subject:
Anopheles; Plasmodium vivax; Primates; cryopreservation; gametocytes; geographical distribution; in vitro culture; malaria; parasites; Brazil; India; United States
Abstract:
... Plasmodium vivax is the most geographically widespread malaria parasite. Unique features of transmission biology complicate P. vivax control. Interventions targeting transmission are required for malaria eradication. In the absence of an in vitro culture, transmission studies rely on live isolates from non-human primates or endemic regions. Here, we demonstrate P. vivax gametocytes from both India ...
DOI:
10.1016/j.ijpara.2016.06.003
PubMed:
27456552
PubMed Central:
PMC5028309

25. Invited Review: Advantages of current and future reproductive technologies for beef cattle production1,21Sincere appreciation is expressed to F. Ciriaco, G. de Moraes, M. Garcia, N. Oosthuizen, and T. Schulmeister for their assistance with reviewing and providing input for this manuscript. In addition, portions of the content published in this manuscript have been reprinted with kind permission from Springer Science + Business Media: Dahlen et al., 2013.2This article is based on a presentation by the authors in the ARPAS Symposium “Reproductive efficiency of beef cows—Current status and new technologies” at the Joint Annual Meeting of ADSA and ASAS in Orlando, Florida, July 14, 2015. Sponsorship of this publication by QualiTech and Micronutrients is appreciated. The ARPAS Foundation is also acknowledged for financial support.

Author:
G.C. Lamb; V.R.G. Mercadante; D.D. Henry; P.L.P. Fontes; C.R. Dahlen; J.E. Larson; N. DiLorenzo
Source:
˜The œprofessional animal scientist 2016 v.32 no.2 pp. 162-171
ISSN:
1525-318X
Subject:
artificial insemination; beef; beef cattle; business enterprises; calves; cattle production; climate; cryopreservation; embryo transfer; environmental impact; estrus; estrus synchronization; females; funding; in vitro fertilization; infrastructure; markets; ovulation; production technology; progeny; reproductive efficiency; semen; sex determination; spermatozoa; Brazil; Florida
Abstract:
... The refinement of current and development of new technologies aimed at increasing the productivity of resources while minimizing the environmental impact will be critical to meet the global food demand in the near future. During the past 50 yr, assisted reproductive technologies have been developed and refined to increase the prolificacy and quality of calves from beef females. Artificial insemina ...
DOI:
10.15232/pas.2015-01455

26. 45 acrosome reaction and heterologous zona binding assay of frozen stallion sperm after hyperactivation

Author:
M. A. Lagares; H. S. Martins; M. R. Souza; C. F. A. M. Penna; F. O. P. Leme; G. C. Silva; S. F. Cortes; R. Stahlberg
Source:
Reproduction, fertility, and development 2016 v.28 no.2 pp. 152
ISSN:
1031-3613
Subject:
acrosome; acrosome reaction; agglutinins; analysis of variance; animal ovaries; antioxidants; calcium; carbon dioxide; catalase; cattle; coculture; cryopreservation; flow cytometry; fluorescein; fluorescence; freeze-thaw cycles; funding; in vitro fertilization; lactoferrin; microscopy; mineral oil; oocytes; peanuts; probability; procaine; propidium; seminal plasma; slaughterhouses; sperm capacitation; stallions; thawing; zona pellucida; Brazil
Abstract:
... During cryopreservation and due to the large portion of seminal plasma removal, there is a decrease in equine sperm antioxidant protection. Lactoferrin and catalase in seminal plasma play an antioxidant role. The fertilizing ability of equine sperm has been analysed in vitro using sperm-zona binding assays with heterologous oocytes. The results have been correlated with in vivo fertility by means ...
DOI:
10.1071/RDv28n2Ab44

27. Cryopreservation of shoot tips excised from zygotic embryos of Araucaria angustifolia (Bertol.) Kuntze

Author:
D. O. Prudente; R. Paiva; P. D. O. Paiva; L. C. Silva
Source:
Acta horticulturae 2016 no.1113 pp. 257-264
ISSN:
0567-7572
Subject:
Araucaria angustifolia; benzyladenine; cryopreservation; cryoprotectants; explants; growth chambers; nitrogen; photoperiod; recalcitrant seeds; regrowth; shoot tips; sucrose; toxicity testing; vitrification; Brazil
Abstract:
... Araucaria angustifolia (Bertol.) Kuntze is the only species of the genus Araucaria naturally occurring in Brazil. The species has recalcitrant seeds, and is in the vulnerable category of the IUCN endangered list. This work presents a protocol for the in vitro conservation of A. angustifolia through the cryopreservation of apical shoot tips excised from zygotic embryos. In vitro regrowth of shoot t ...
DOI:
10.17660/ActaHortic.2016.1113.38

28. Sperm morphometry and chromatin condensation in Nelore bulls of different ages and their effects on IVF

Author:
B.H. Kipper; J.T. Trevizan; J.T. Carreira; I.R. Carvalho; G.Z. Mingoti; M.E. Beletti; S.H.V. Perri; D.A. Franciscato; J.C. Pierucci; M. B Koivisto
Source:
Theriogenology 2017 v.87 pp. 154-160
ISSN:
0093-691X
Subject:
Nellore; adults; artificial insemination; bulls; chromatin; cryopreservation; embryogenesis; in vitro fertilization; microscopy; morphometry; semen; spermatozoa; staining; toluidine blue; Brazil
Abstract:
... The aim of this study was to evaluate the chromatin packing and sperm head morphometry of cryopreserved semen of Nelore bulls (Bos taurus indicus) of different ages. Furthermore, the influence of the degree of chromatin compaction on in vitro embryo production (IVP) was investigated. Forty bulls were divided into three groups: young (1.8–2 years), adult (3.5–7 years), and senile (8–14.3 years). Th ...
DOI:
10.1016/j.theriogenology.2016.08.017

29. Cryopreservation and optimization of in vitro germination of handroanthus serratifolius

Author:
A. C. Souza; R. Paiva; L. C. Silva; P. A. A. Santos; A. A. Alvarenga; F. C. Moreira; D. P. Vargas
Source:
Acta horticulturae 2015 no.1083 pp. 481-486
ISSN:
0567-7572
Subject:
Handroanthus serratifolius; alcohols; chlorine; cryopreservation; decontamination; falcons; germination; nitrogen; ornamental plants; roots; seedlings; seeds; shoots; sodium hypochlorite; thawing; trees; viability; Brazil
Abstract:
... Handroanthus serratifolius is a tree species with wide distribution in Brazil which may be used as ornamental plant. It is propagated by seeds which loose viability within a short period. This study aimed to (i) test different thawing periods for cryopreserved seeds, (ii) evaluate the germination rate, fresh and dry weights of the seedlings, and (iii) optimize the in vitro germination process. See ...
DOI:
10.17660/ActaHortic.2015.1083.62

30. Effect of load solution and growth media on cryopreservation by droplet Vitrification of Hancornia speciose shoot tips

Author:
P. A. A. Santos; R. Paiva; L. C. Silva; A. C. Souza; C. M. Gallo; R. C. Herrera
Source:
Acta horticulturae 2015 no.1083 pp. 507-512
ISSN:
0567-7572
Subject:
ascorbic acid; benzyladenine; cryopreservation; culture media; explants; exposure duration; naphthaleneacetic acid; nitrogen; polyvinylpyrrolidone; recalcitrant seeds; regrowth; sucrose; thawing; vitrification; woody plants; Brazil
Abstract:
... Hancornia speciosa Gomes is a native fruit species from Brazil which produces recalcitrant seeds and thus, conservation methods must be studied in order to preserve the species. The objectives of this study were: (i) to subject shoot tips to different exposure periods to loading solution and, (ii) test different regeneration culture media for cryopreserved explants. Shoot tips were excised from in ...
DOI:
10.17660/ActaHortic.2015.1083.66

31. Innovative approaches to the preservation of forest trees

Author:
Hugh W. Pritchard; Justin F. Moat; João B.S. Ferraz; Timothy R. Marks; José Luís C. Camargo; Jayanthi Nadarajan; Isolde D.K. Ferraz
Source:
Forest ecology and management 2014 v.333 pp. 88-98
ISSN:
0378-1127
Subject:
at-risk population; biodiversity; biogeography; biotechnology; cryopreservation; databases; drought tolerance; education; extinction; forest trees; forestry; genetic variation; geographic information systems; horticulture; in situ conservation; information systems; issues and policy; longevity; models; nongovernmental organizations; progeny; recalcitrant species; seed storage; seeds; viability; Brazil; China
Abstract:
... The recent acceleration of actions to conserve plant species using ex situ and in situ strategies has revealed the need to understand how these two approaches might be better developed and integrated in their application to tree species. Here we review some of the recent successes relating mainly to tree seed biology that have resulted in the development and application of innovative actions acros ...
DOI:
10.1016/j.foreco.2014.08.012

32. Trypanosoma cruzi and Leishmania infantum chagasi Infection in Wild Mammals from Maranhão State, Brazil

Author:
da Costa Andréa Pereira; Costa Francisco Borges; Soares Herbert Sousa; Ramirez Diego Garcia; de Carvalho Mesquita Eric Takashi Kamakura; Gennari Solange Maria; Marcili Arlei
Source:
Vector borne and zoonotic diseases 2015 v.15 no.11 pp. 656-666
ISSN:
1557-7759
Subject:
Leishmania donovani; phylogeny; Chiroptera; Leishmania infantum; genes; domestic animals; blood; ribosomal DNA; wild animals; Trypanosoma cruzi; human diseases; hematophagous arthropods; polymerase chain reaction; glyceraldehyde-3-phosphate dehydrogenase; fluorescent antibody technique; Didelphimorphia; serodiagnosis; cryopreservation; semiarid zones; synanthropes; cytochrome b; opossums; rodents; Brazil
Abstract:
... Trypanosoma and Leishmania are obligate parasites that cause important diseases in human and domestic animals. Wild mammals are the natural reservoirs of these parasites, which are transmitted by hematophagous arthropods. The present study aimed to detect the natural occurrence of trypanosomatids through serological diagnosis, PCR of whole blood and blood culture (hemoculture), and phylogenetic re ...
DOI:
10.1089/vbz.2015.1771

33. C-2013

Author:
G. Pacheco; T. Merhy; M. Vianna; R. Garcia; E. Mansur
Source:
Cryobiology 2014 v.69 no.3 pp. 519
ISSN:
0011-2240
Subject:
Passiflora; calcium alginate; cryopreservation; cryoprotectants; disease resistance; encapsulation; explants; fruit growing; genetic stability; genomics; germplasm; indigenous species; liquids; microsatellite repeats; passion fruits; random amplified polymorphic DNA technique; shoots; soil-borne diseases; storage time; sucrose; vitrification; Brazil
Abstract:
... Passiflora pohlii Mast. (Passifloraceae) is a wild species native to Brazil, with a potential agronomic interest due to its tolerance to soil-borne pathogens that cause damage to the passion fruit culture. Because this species occurs in impacted regions, the goal of this study was the development of an efficient cryopreservation strategy for nodal segments excised from axenic plants using vitrific ...
DOI:
10.1016/j.cryobiol.2014.09.360

34. Comparative evaluation of different preservation methods for cyanobacterial strains

Author:
Alberto A. Esteves-Ferreira; Débora M. Corrêa; Antônio P. S. Carneiro; Rinamara M. Rosa; Robson Loterio; Wagner L. Araújo
Source:
Journal of applied phycology 2013 v.25 no.4 pp. 919-929
ISSN:
0921-8971
Subject:
Cyanobacteria; cell viability; cryopreservation; cytotoxicity; dimethyl sulfoxide; freeze drying; genetic drift; genetic stability; glycerol; plant biology; plant exploration and collection; risk; storage time; Brazil
Abstract:
... Maintaining pure cultures using preservation methods is of high importance for biotechnological purposes. However, preservation does not necessarily guarantee the genetic stability of these cultures. Therefore, preservation methods are currently needed to assure viability as well as genetic, physiological, and morphological integrity across storage periods. In this study, preservation of five isol ...
DOI:
10.1007/s10811-012-9927-9

35. Effects of extenders, cryoprotectants and freezing methods on sperm quality of the threatened Brazilian freshwater fish pirapitinga-do-sul Brycon opalinus (Characiformes)

Author:
A.T.M. Viveiros; L.H. Orfão; A.F. Nascimento; F.M. Corrêa; D. Caneppele
Source:
Theriogenology 2012 v.78 no.2 pp. 361-368
ISSN:
0093-691X
Subject:
Characiformes; conservation programs; cryopreservation; cryoprotectants; dimethyl sulfoxide; eggs; freezing; freshwater fish; glucose; hatching; larvae; nitrogen; sodium chloride; spermatozoa; temperature; thawing; threatened species; vapors
Abstract:
... The objective was to develop a suitable freezing method to cryopreserve Brycon opalinus (Characiformes) sperm. Extenders (NaCl and glucose at 325 and 365 mOsm/kg), cryoprotectants (dimethyl sulfoxide = dimethyl sulfoxide (DMSO) and methyl glycol = methyl glycol (MG)), equilibration times (15 and 30 min), thawing temperatures (30 and 60 °C), and straw sizes (0.5 and 4.0 mL) were tested. Sperm were ...
DOI:
10.1016/j.theriogenology.2012.02.015
PubMed:
22537997

36. Improvements in obtaining New World Leishmania sp from mucosal lesions: Notes on isolating and stocking parasites

Author:
Miriam L. Dorta; Milton A.P. Oliveira; Anna K.A. Fleuri; Fernanda B. Duarte; Sebastião A. Pinto; Ledice I.A. Pereira; Fatima Ribeiro-Dias
Source:
Experimental parasitology 2012 v.132 no.2 pp. 300-303
ISSN:
0014-4894
Subject:
Leishmania; animal models; biomarkers; biopsy; cryopreservation; culture media; cutaneous leishmaniasis; genetic variation; host-parasite relationships; isolation techniques; mice; parasites; parasitology; patients; Brazil
Abstract:
... Tegumentary leishmaniasis is an endemic protozoan disease that, in Brazil, is caused by parasites from Viannia or Leishmania complex. The clinical forms of cutaneous disease comprise localized, disseminated, mucosal or mucocutaneous, and diffuse leishmaniasis. Viannia complex parasites are not easy to isolate from patient lesions, especially from mucosal lesions, and they are difficult to culture. ...
DOI:
10.1016/j.exppara.2012.06.006

37. Impact of proximal cytoplasmic droplets on quality traits and in-vitro embryo production efficiency of cryopreserved bull spermatozoa

Author:
Janaina T Carreira; Gisele Z Mingoti; Lucia H Rodrigues; Carlos Silva; Silvia HV Perri; Marion B Koivisto
Source:
Acta veterinaria scandinavica 2012 v.54 no.1 pp. 760
ISSN:
1751-0147
Subject:
acridine orange; acrosome; bulls; correlation; cryopreservation; cytoplasm; droplets; early development; embryogenesis; hatching; in vitro fertilization; microscopy; semen; sperm motility; vigor; zebu
Abstract:
... BACKGROUND: Proximal cytoplasmic droplets (PCDs), a remnant of germ cell cytoplasm, are common non-specific morphological defects in bovine semen. This study evaluated the effect of higher percentages of PCDs on the quality of frozen-thawed bovine semen, embryo production and early embryo development. METHODS: Three ejaculates from each of five (group 1: PCD ≤ 1%, control) and eight adult Bos indi ...
DOI:
10.1186/1751-0147-54-1
PubMed:
22240071
PubMed Central:
PMC3292455

38. Sperm cryopreservation affects postthaw motility, but not embryogenesis or larval growth in the Brazilian fish Brycon insignis (Characiformes)

Author:
A.T.M. Viveiros; Z.A. Isaú; D. Caneppele; M.C. Leal
Source:
Theriogenology 2012 v.78 no.4 pp. 803-810
ISSN:
0093-691X
Subject:
Characiformes; animal growth; body weight; cryopreservation; embryogenesis; endangered species; fish; hatching; larvae; larval development; nitrogen; progeny; sperm motility; spermatozoa; thawing; vapors
Abstract:
... Sperm cryopreservation is an important method for preserving genetic information and facilitating artificial reproduction. The objective was to investigate whether the cryopreservation process affects postthaw sperm motility, embryogenesis, and larval growth in the fish Brycon insignis. Sperm was diluted in methyl glycol and Beltsville Thawing solution, frozen in a nitrogen vapor vessel (dry shipp ...
DOI:
10.1016/j.theriogenology.2012.03.028
PubMed:
22541324

39. Effect of DMSO on castor bean pollen grain viability

Author:
Vargas, D.P.; Paiva, R.; Silva, D.P.C. da; Campos, A.C.A.L.; Nogueira, G.F.; Carvalho, M.L.M. de
Source:
Acta horticulturae 2011 v. no.908 pp. 93-96
ISSN:
0567-7572
Subject:
viability; male flowers; castor beans; cryopreservation; nitrogen; dimethyl sulfoxide; Ricinus communis; storage temperature; biodiesel; pollen; economic valuation; Brazil
Abstract:
... Castor bean (Ricinus communis L.) is an oleaginous plant of high economic value in Brazil due to its use in the industrial (biodiesel production) and medicinal areas. The objective of this study was to evaluate the effect of the use of the cryoprotectant DMSO on the maintenance of pollen grain integrity under different storage temperatures. Pollen grains were extracted from anthers of male flowers ...

40. Effect of glycerol as a cryoprotectant for castor bean pollen for different storage periods

Author:
Vargas, D.P.; Paiva, R.; Nogueira, G.F.; Carvalho, M.A.F.; Silva, D.P.C. da; Nery, F.C.; Porto, J.M.P.; Silva, G.A. da
Source:
Acta horticulturae 2011 v. no.908 pp. 97-100
ISSN:
0567-7572
Subject:
Ricinus communis; biodiesel; castor beans; cryopreservation; cultivars; dyes; glycerol; nitrogen; pollen; storage time; temperature; viability; water content; Brazil
Abstract:
... The cultivation of castor bean (Ricinus communis) in Brazil has been considered strategic due to the enormous increase of its use in the production of biodiesel. Cryopreservation shows a great potential to preserve cultivars or varieties of this species. Considering the importance of using pollen grains as conservation elements of genetic resources, the objective of this study was to evaluate the ...

41. In vitro and cryogenic preservation of plant biodiversity in Brazil

Author:
Pilatti, Fernanda Kokowicz; Aguiar, Tarsis; Simões, Thiago; Benson, Erica E.; Viana, Ana Maria
Source:
In vitro cellular & developmental biology 2011 v.47 no.1 pp. 82-98
ISSN:
1054-5476
Subject:
biodiversity; cerrado; climate change; cryopreservation; deforestation; economic valuation; ecosystems; forests; genetic background; genetic variation; germplasm; habitats; indigenous species; labor; land use; risk; Brazil; Pantanal
Abstract:
... The Brazilian biomes (Amazon, Atlantic Forest, Cerrado, Caatinga, Pantanal, and Pampa) comprise one of the highest levels of plant diversity in the world; however, non-sustainable practices, deforestation, and land use have resulted in significant losses and fragmentation of the native forests. These ecosystems are now threatened and protection of their native plants through ex situ conservation i ...
DOI:
10.1007/s11627-010-9302-y

42. Sperm quality and cryopreservation of Brazilian freshwater fish species: a review

Author:
Viveiros, A. T. M.; Godinho, H. P.
Source:
Fish physiology and biochemistry 2009 v.35 no.1 pp. 137-150
ISSN:
0920-1742
Subject:
breeding; cryopreservation; deforestation; extinction; freezing; freshwater fish; gene banks; genetic variation; hatcheries; hatching; indigenous species; males; pollution; spawning; sperm motility; spermatozoa; urbanization; Brazil
Abstract:
... The Brazilian freshwater fish diversity is the richest in the world. Only 0.7% of all Brazilian species have had any aspect of their sperm biology addressed up to this date. The majority of the fish species described in this review migrate during the spawning season (a phenomenon known as piracema). Urbanization, pollution, hydroelectric dams and deforestation are some of the causes of stock deple ...
DOI:
10.1007/s10695-008-9240-3
PubMed:
19189240

43. Gene banking of the neotropical fish Leporinus obtusidens (Valenciennes, 1836): A protocol to freeze its sperm in the field

Author:
Taitson, P.F.; Chami, E.; Godinho, H.P.
Source:
Animal reproduction science 2008 v.105 no.3-4 pp. 283-291
ISSN:
0378-4320
Subject:
milt; viability; freeze drying; cryopreservation; glucose; semen banks; tropics; dimethyl sulfoxide; sperm motility; cryoprotectants; fertilization (reproduction); egg yolk; water; male fertility; spermatozoa; gene banks; fish; transporting quality; germplasm conservation; Brazil
Abstract:
... A practical sperm cryopreservation protocol using a dry-shipper and a diluent of simple composition is described for the neotropical fish Leporinus obtusidens (Valenciennes, 1836). The cooling rate of the dry-shipper and its period of useful time, established under laboratory conditions, were respectively 25.7-30.8°C/min (between 0 and -60°C) and 9 days after charging. Sperm donors were selected o ...
DOI:
10.1016/j.anireprosci.2007.03.009

44. Pulmonary cryptococcosis in slaughtered sheep: Anatomopathology and culture

Author:
Lemos, L.S.; Santos, A.S. de O. dos; Vieira-da-Motta, O.; Texeira, G.N.; Carvalho, E.C.Q. de
Source:
Veterinary microbiology 2007 v.125 no.3-4 pp. 350-354
ISSN:
0378-1135
Subject:
sheep; sheep diseases; respiratory tract diseases; mycoses; Cryptococcus neoformans; disease detection; case studies; epidemiological studies; pathogen identification; lungs; tissue analysis; cryopreservation; histopathology; yeasts; epithelium; viability; microscopy; differential staining; in vitro culture; risk assessment; disease transmission; zoonoses; Brazil
Abstract:
... Cryptococcosis is considered a zoonosis but is rare in stock animals. In humans it is imputed to be a mortality cause in immunocompromised patients. This case study reports the anatomopathology of respiratory cryptococcosis in ovine and the recuperation and identification of Cryptococcus neoformans var. neoformans from 1-year frozen lung tissue sample. The findings from haematoxylin/eosin staining ...
DOI:
10.1016/j.vetmic.2006.11.028

45. In vitro conservation of Cedrela fissilis Vellozo (Meliaceae), a native tree of the Brazilian Atlantic Forest

Author:
Nunes, E. da C.; Benson, E.E.; Oltramari, A.C.; Araujo, P.S.; Moser, J.R.; Viana, A.M.
Source:
Biodiversity and conservation 2003 v.12 no.4 pp. 837-848
ISSN:
0960-3115
Subject:
germplasm conservation; artificial seeds; encapsulation; cotyledons; explants; shoots; cryopreservation; Brazil

46. Analise comparativa da estabilidade do DNA de Dalbulus maidis (DeLong & Wolcott) (Hemiptera: Cicadellidae) sob diferentes metodos de preservacao para uso em RAPD-PCR. [Erratum: July/Sept 2002, v. 31 (3), p. unnumbered.]

Author:
Oliveira, C.M.; Fungaro, M.H.P.; Camargo, L.E.A.; Lopes, J.R.S.
Source:
Neotropical entomology 2002 v.31 no.2 pp. 225-231
ISSN:
1519-566X
Subject:
random amplified polymorphic DNA technique; Zea mays; insect pests; shelf life; cryopreservation; DNA; corn; genetic stability; air drying; temperature; Dalbulus maidis; ethanol; Brazil
Abstract:
... The appropriate preservation of the DNA of a certain organism is important for successful application of molecular techniques like RAPD-PCR. This study was designed to compare simple methods of preservation of specimens of Dalbulus maidis (DeLong & Wolcott) (Hemiptera: Cicadellidae) with respect to quantity and quality of DNA for use in RAPD-PCR, after different storage periods. Eight methods were ...
DOI:
10.1590/S1519-566X2002000200008