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- Author:
- Taberner, E.; Morató, R.; Mogas, T.; Miró, J.
- Source:
- Animal reproduction science 2010 v.118 no.2-4 pp. 354-361
- ISSN:
- 0378-4320
- Subject:
- viability; pronucleus; laboratory techniques; cryopreservation; zona pellucida; accuracy; males; sperm motility; velocity; fertilization (reproduction); asses; cows; spermatozoa; thawing; oocytes; cell adhesion; in vitro studies
- Abstract:
- ... An experiment was designed to study the interaction between fresh/frozen-thawed donkey spermatozoa and zona pellucida (ZP)-free bovine oocytes in an attempt to develop a model for assessing cryopreserved Catalonian donkey sperm function. Semen from five donkeys was collected using an artificial vagina. Sperm motility and viability were immediately assessed and the semen sample cryopreserved. Sperm ...
- DOI:
- 10.1016/j.anireprosci.2009.08.005
- PubMed:
- 19748750
-
http://dx.doi.org/10.1016/j.anireprosci.2009.08.005
- Author:
- Lemoine, M.; Mignon-Grasteau, S.; Grasseau, I.; Magistrini, M.; Blesbois, E.
- Source:
- Theriogenology 2011 v.75 no.1 pp. 122-130
- ISSN:
- 0093-691X
- Subject:
- acrosome reaction; chickens; computers; cryopreservation; flocks; freezing; semen; sperm motility; spermatozoa; viability
- Abstract:
- ... The effects of in vitro storage on the sperm's ability to undergo the acrosome reaction (AR) have never been studied in avian species despite its major importance for reproduction management. The ability of chicken sperm to undergo the AR was measured after liquid storage at 4 °C and after cryopreservation, and its relationship with other semen quality parameters, including viability, mass motilit ...
- DOI:
- 10.1016/j.theriogenology.2010.07.017
-
http://dx.doi.org/10.1016/j.theriogenology.2010.07.017
- Author:
- Chantzaropoulos, A.; Nathanailides, C.; Kokokiris, L.; Barbouti, A.; Zhang, T.
- Source:
- Journal of applied ichthyology 2015 v.31 Suppl S1 pp. 89-93
- ISSN:
- 0175-8659
- Subject:
- Carassius auratus; bags; cold storage; cryopreservation; goldfish; pH; refrigeration; reproduction; spermatozoa; viability
- Abstract:
- ... Optimal conditions of short‐term storage of fish sperm are important for successful artificial reproduction protocols or incubation of sperm in a cryoprotecting medium for successful sperm cryopreservation. The influence of acid or alkaline incubating conditions on short term storage of goldfish sperm was investigated in a series of experiments. Goldfish sperm was diluted an immobilizing solution ...
- DOI:
- 10.1111/jai.12735
-
http://dx.doi.org/10.1111/jai.12735
- Author:
- Naing, Aung Htay; Kim, Chang Kil
- Source:
- 3 Biotech 2019 v.9 no.9 pp. 329
- ISSN:
- 2190-572X
- Subject:
- adsorption; antifreeze proteins; cold tolerance; cold zones; crops; cryopreservation; crystals; fish; food technology; frost protection; gene overexpression; genes; genetic engineering; ice; insects; temperature
- Abstract:
- ... Antifreeze proteins (AFPs) confer the ability to survive at subzero temperatures and are found in many different organisms, including fish, plants, and insects. They prevent the formation of ice crystals by non-colligative adsorption to the ice surface and are essential for the survival of organisms in cold environments. These proteins are also widely used for cryopreservation, food technology, an ...
- DOI:
- 10.1007/s13205-019-1861-y
- PubMed:
- 31448185
- PubMed Central:
- PMC6691018
-
https://dx.doi.org/10.1007/s13205-019-1861-y
- Author:
- Maya-Soriano, M.J.; Taberner, E.; Sabés-Alsina, M.; Piles, M.; Lopez-Bejar, M.
- Source:
- Zygote 2015 v.23 no.1 pp. 1-10
- ISSN:
- 1469-8730
- Subject:
- New Zealand White rabbit; acrosome; antioxidants; artificial insemination; bovine serum albumin; bucks; cryopreservation; freezing; rabbits; semen; sperm quality; staining; thawing; toxicity; viability; vitamin A
- Abstract:
- ... The generation of reactive oxygen species associated with cryopreservation could be responsible for mammalian sperm damage and the limitable value of stored semen in artificial insemination. The aim of this study was to assess several antioxidant agents supplemented in a commercial freezing extender (Gent B®) in order to improve post-thaw rabbit sperm quality. Ejaculates of 26 New Zealand White ra ...
- DOI:
- 10.1017/S0967199413000270
- PubMed:
- 23965603
-
https://dx.doi.org/10.1017/S0967199413000270
- Author:
- Lewis, Jedediah; Morigi, Valentina; Giwa, Sebastian; Allal, Zak; Tocchio, Alessandro
- Source:
- Cryobiology 2015 v.71 no.1 pp. 178
- ISSN:
- 0011-2240
- Subject:
- animal ovaries; blood vessels; business enterprises; chilling injury; cryopreservation; cryoprotectants; ecosystems; entrepreneurship; experts; government agencies; heart valves; humans; ice; issues and policy; kidneys; liver; markets; patients; physicians; public health; rabbits; rats; sheep; shelf life; spermatozoa; stem cells; swine; temperature; tissue banks; tissue engineering; toxicity; vitrification
- Abstract:
- ... The Organ Preservation Alliance is a non-profit foundation incubated at SU labs at NASA Research Park in silicon valley and lead by an interdisciplinary team of scientists, doctors, policy influencers, and entrepreneurs. The Alliance aims to catalyze breakthroughs in the long-term preservation of human organs and other tissue systems. This will save and enrich the lives of millions of patients who ...
- DOI:
- 10.1016/j.cryobiol.2015.05.060
-
http://dx.doi.org/10.1016/j.cryobiol.2015.05.060
- Author:
- Santiago-Moreno, J.; Gil, M.G.; Dávila, S.G.; Campo, J.L.; Castaño, C.; Toledano-Díaz, A.; Prieto, M.T.; Blesbois, E.
- Source:
- Poultry science 2018 v.97 no.12 pp. 4433-4441
- ISSN:
- 0032-5791
- Subject:
- acrosome; chicken breeds; cryopreservation; freeze-thaw cycles; grazing; heterophils; paralysis; pastures; roosters; semen; sperm motility; sperm quality
- Abstract:
- ... The aim of the present work was to examine the influence of access to pasture in an outdoor housing system on rooster sperm quality and response to cryopreservation and to examine the possible correlation between values for sperm quality variables and welfare indicators. Two groups of Black-barred Andaluza and Red-barred Vasca roosters were housed in an outdoor system, with one group given daily a ...
- DOI:
- 10.3382/ps/pey299
-
https://dx.doi.org/10.3382/ps/pey299
- Author:
- Funnekotter, Bryn; Kaczmarczyk, Anja; Turner, Shane R.; Bunn, Eric; Zhou, Wenxu; Smith, Steven; Flematti, Gavin; Mancera, Ricardo L.
- Source:
- Plant cell, tissue, and organ culture 2013 v.114 no.1 pp. 83-96
- ISSN:
- 0167-6857
- Subject:
- Arabidopsis thaliana; Grevillea; acclimation; cell membranes; cold; cryopreservation; droplets; explants; indigenous species; oxidative stress; phospholipids; shoots; sterols; sugars; temperature; vitrification
- Abstract:
- ... Cell membranes are the primary sites of cryopreservation injury and measuring changes to membrane composition arising from cold acclimation may assist with providing a rationale for optimising cryopreservation methods. Shoot tips from two south-west Western Australian species, Grevillea scapigera and Loxocarya cinerea, and Arabidopsis thaliana (reference species) were subjected to cryopreservation ...
- DOI:
- 10.1007/s11240-013-0308-0
-
http://dx.doi.org/10.1007/s11240-013-0308-0
- Author:
- Abe, H.; Yamashita, S.; Satoh, T.; Hoshi, H.
- Source:
- Gamete research 2002 v.61 no.1 pp. 57-66
- ISSN:
- 1040-452X
- Subject:
- cattle; lipids; cytoplasm; culture media; oocytes; cell culture; embryogenesis; cryopreservation; embryo (animal)
- DOI:
- 10.1002/mrd.1131
- PubMed:
- 11774376
-
http://dx.doi.org/10.1002/mrd.1131
- Author:
- Zarletti, Gianpaolo; Tiberi, Massimo; De Molfetta, Veronica; Bossù, Maurizio; Toppi, Elisa; Bossù, Paola; Scapigliati, Giuseppe
- Source:
- Viruses 2020 v.12 no.11
- ISSN:
- 1999-4915
- Subject:
- B-lymphocytes; COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; antibodies; blood serum; cryopreservation; enzyme-linked immunosorbent assay; humoral immunity; immune response; immunogenicity; immunoglobulin G; patients; reverse transcriptase polymerase chain reaction; risk; screening; serodiagnosis; seroprevalence; vaccination; viruses
- Abstract:
- ... Knowledge of the antibody-mediated immune response to SARS-CoV-2 is crucial to understand virus immunogenicity, establish seroprevalence, and determine whether subjects or recovered patients are at risk for infection/reinfection and would therefore benefit from vaccination. Here, we describe a novel and simple cell-ELISA specifically designed to measure viral spike S1-specific IgG produced in vitr ...
- DOI:
- 10.3390/v12111274
-
http://dx.doi.org/10.3390/v12111274
- Author:
- da Silva, Aleksandro S.; Monteiro, Silvia G.; Gonçalves, Jamile F.; Spanevello, Rosélia; Oliveira, Camila B.; Costa, Marcio M.; Jaques, Jeandre A.S.; Morsch, Vera M.; Schetinger, Maria Rosa C.; Mazzanti, Cinthia M.; Lopes, Sonia T.A.
- Source:
- Veterinary parasitology 2011 v.175 no.3-4 pp. 237-244
- ISSN:
- 0304-4017
- Subject:
- Trypanosoma evansi; acetylcholine; acetylcholinesterase; blood; cerebellum; cerebral cortex; cryopreservation; hippocampus; lipid peroxidation; parasites; polymerase chain reaction; rats; spinal cord; thiobarbituric acid-reactive substances; trypomastigotes; vertebrae
- Abstract:
- ... Neurological and locomotor clinical signs are described in animals infected with Trypanosoma evansi. These disturbances may be related to changes in the amount of acetylcholine (neurotransmitter) in the synaptic cleft. Therefore, changes in acetylcholinesterase (AChE) activity and lipid peroxidation in brain and spinal cord of T. evansi-infected rats were investigated. Each rat was intraperitoneal ...
- DOI:
- 10.1016/j.vetpar.2010.10.021
- PubMed:
- 21055876
-
http://dx.doi.org/10.1016/j.vetpar.2010.10.021
- Author:
- Mullen, S.F.; Fahy, G.M.
- Source:
- Theriogenology 2012 v.78 no.8 pp. 1709-1719
- ISSN:
- 0093-691X
- Subject:
- cattle; chilling injury; cooling; cryopreservation; cytoskeleton; messenger RNA; nitrogen; oocytes; sheep; stress tolerance; swine; vitrification
- Abstract:
- ... Vitrification as a means of cryopreservation has become a standard approach for oocytes from livestock. This paradigm shift occurred primarily as a result of the demonstration in 1996 that bovine oocytes are extremely susceptible to chilling injury. Since that early work, numerous devices have been used as supports for oocytes during so-called “ultra-rapid cooling”, and occasionally, trials involv ...
- DOI:
- 10.1016/j.theriogenology.2012.06.008
- PubMed:
- 22968034
-
http://dx.doi.org/10.1016/j.theriogenology.2012.06.008
- Author:
- Thitilertdecha, Premrutai; Suwannachod, Pornpichaya; Poungpairoj, Poonsin; Tantithavorn, Varangkana; Khowawisetsut, Ladawan; Ammaranond, Palanee; Onlamoon, Nattawat
- Source:
- Journal of immunological methods 2018
- ISSN:
- 0022-1759
- Subject:
- CD4-positive T-lymphocytes; HIV infections; Macaca; bags; cell proliferation; cell viability; cryopreservation; culture flasks; immunotherapy; patients; phenotype; protocols
- Abstract:
- ... CD4 immunotherapy is potentially useful in immune reconstitution of CD4⁺ T cells for HIV-infected patients. Transfusion of anti-CD3/28 expanded CD4⁺ T cells is also proved to be safe and effective in both SIV-infected macaques and HIV-infected patients. However, there is no such standardized and practical protocol available for cell production in order to use in clinics. This study thus aimed to d ...
- DOI:
- 10.1016/j.jim.2018.06.004
-
https://dx.doi.org/10.1016/j.jim.2018.06.004
- Author:
- Husna, Asma Ul; Ejaz, Rabea; Qadeer, Saima; Azam, Asima; Rakha, Bushra Allah; Ansari, Muhammad Sajjad; Shahzad, Qaisar; Javed, Moazzam; Vazquez-Levin, Mónica H.; Akhter, Shamim
- Source:
- Animal reproduction science 2016 v.174 pp. 29-36
- ISSN:
- 0378-4320
- Subject:
- Sahiwal; buffaloes; bulls; cell membranes; chromatin; cryopreservation; filtration; glass fibers; in vitro fertilization; semen; spermatozoa; viability; Pakistan
- Abstract:
- ... Sperm selection techniques have been developed to get sperm suspensions enriched in motile and functional cells. Studies show that selection before cryopreservation improves post-thaw quality of cryopreserved sperm but information on buffalo bull sperm is scarce. The study was aimed to 1) perform a comparative analysis of sperm selection procedures; Swim-Up (SU), Sephadex™-G15 Filtration (S-G15) o ...
- DOI:
- 10.1016/j.anireprosci.2016.08.015
-
https://dx.doi.org/10.1016/j.anireprosci.2016.08.015
- Author:
- Do, Van Huong; Walton, Simon; Catt, Sally; Taylor-Robinson, Andrew W.
- Source:
- Cryobiology 2017 v.77 pp. 58-63
- ISSN:
- 0011-2240
- Subject:
- blastocyst; cattle; cryopreservation; freezing; hatching; in vitro culture; thawing; vitrification; zygote
- Abstract:
- ... The effectiveness of three cryopreservation protocols (slow freezing, short equilibration vitrification and long equilibration vitrification) on in vitro-derived cattle embryos at expanded blastocyst and pronuclear stages was compared. 199 expanded blastocysts of good quality were assigned randomly into four treatment groups [control, non-cryopreserved (fresh, unfrozen); and the three cryopreserva ...
- DOI:
- 10.1016/j.cryobiol.2017.05.007
-
http://dx.doi.org/10.1016/j.cryobiol.2017.05.007
- Author:
- Gu, Yi-Fan; Lu, Chang-Fu; Lin, Ge; Lu, Guang-Xiu
- Source:
- Reproduction 2010 v.139 no.1 pp. 121-127
- ISSN:
- 1470-1626
- Subject:
- correlation; cryopreservation; embryo transfer; glycoproteins; humans; in vitro fertilization; light microscopy; patients; polarized light; pregnancy; quantitative analysis; zona pellucida
- Abstract:
- ... The cryopreservation of human embryos is thought to induce alteration in the glycoprotein matrix and lead to zona change. However, this assumption has been full of controversies till now. The objective of this study was to evaluate the effect of cryopreservation on zona pellucida of human embryos. Fresh (n=106, from 40 patients) and frozen-thawed embryos (n=123, from 40 patients) were obtained fro ...
- DOI:
- 10.1530/REP-09-0227
- PubMed:
- 19736257
-
http://dx.doi.org/10.1530/REP-09-0227
- Author:
- Bonga, J.M.
- Source:
- Canadian journal of forest research = 2014 v.45 no.4 pp. 379-383
- ISSN:
- 1208-6037
- Subject:
- conifers; cryopreservation; genetic improvement; germination; industrial applications; juveniles; organogenesis; rooting; somatic embryogenesis; vegetative propagation
- Abstract:
- ... Vegetative propagation of conifers has found large-scale industrial application via somatic embryogenesis (SE), rooting of cuttings, and organogenesis. Genetic gain is achieved with all of these methods but is the highest with SE, primarily because SE cultures can be cryopreserved. This allows for plants derived from part of each cell line to be field tested over a long period while the rest of ea ...
- DOI:
- 10.1139/cjfr-2014-0360
-
http://dx.doi.org/10.1139/cjfr-2014-0360
- Author:
- Clulow, J.R.; Mansfield, L.J.; Morris, L.H.A.; Evans, G.; Maxwell, W.M.C.
- Source:
- Animal reproduction science 2008 v.108 no.3-4 pp. 298-308
- ISSN:
- 0378-4320
- Subject:
- viability; cryopreservation; freezers; freeze-thaw cycles; automation; cryoprotectants; processing equipment; sperm motility; male fertility; acrosome; stallions; germplasm conservation; vapors; in vitro studies
- Abstract:
- ... The effects of sperm freezing concentration (40x10⁶ mL⁻¹ vs. 400x10⁶ mL⁻¹), straw size (0.25mL vs. 0.5mL) and freezing method (liquid nitrogen vapour in a Styrofoam® box vs. programmable freezing machine) were evaluated in a 2x2x2 factorial experimental design using 3 split ejaculates from each of 4 stallions. Immediately after thawing, the total motility and forward progressive motility of sperma ...
- DOI:
- 10.1016/j.anireprosci.2007.08.014
-
http://dx.doi.org/10.1016/j.anireprosci.2007.08.014
