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1. Candidiasis in Birds (Galliformes, Anseriformes, Psittaciformes, Passeriformes, and Columbiformes): A Focus on Antifungal Susceptibility Pattern of Candida albicans and Non-albicans Isolates in Avian Clinical Specimens

Author:
Forough Talazadeh; Masoud Ghorbanpoor; Abolfazl Shahriyari
Source:
Topics in companion animal medicine 2022 v.46 pp. 100598
ISSN:
1938-9736
Subject:
Anseriformes; Candida albicans; Columbiformes; Galliformes; Passeriformes; Psittaciformes; agar; amphotericin B; antibiotic resistance; candidiasis; chloramphenicol; cloaca; cycloheximide; cytochrome P-450; disk diffusion antimicrobial test; fluconazole; fungi; genes; glucose; itraconazole; ketoconazole; medicine; microscopy; nystatin; oligodeoxyribonucleotides; pets; pharynx; Iran
Abstract:
... Candidiasis is a fungal infection caused by Candida species which has been reported in most domestic and wild birds and mammals. In this study, 196 samples from different species of birds with suspected symptoms of candidiasis were examined. Pharyngeal swabs, cloacal swabs, and fecal samples were taken from the birds. The samples were cultured in sabouraud dextrose agar (SDA) containing cyclohexim ...
DOI:
10.1016/j.tcam.2021.100598

2. Synergy between fungi and bacteria promotes polycyclic aromatic hydrocarbon cometabolism in lignin-amended soil

Author:
Decheng Gu; Xingjia Xiang; Yucheng Wu; Jun Zeng; Xiangui Lin
Source:
Journal of hazardous materials 2022 v.425 pp. 127958
ISSN:
0304-3894
Subject:
Basidiomycota; Methylophilaceae; Sphingomonadaceae; anthracenes; biodegradation; bioremediation; carbon dioxide; cycloheximide; fungi; isotope labeling; lignin; mineralization; models; soil
Abstract:
... Lignin enhanced biodegradation of polycyclic aromatic hydrocarbons (PAHs) in soil, but collaboration among soil microorganisms during this process remains poorly understood. Here we explored the relations between microbial communities and PAH transformation in soil microcosms amended with lignin. Mineralization of the four-ring benzo(a)anthracene (BaA), which was selected as a model, was determine ...
DOI:
10.1016/j.jhazmat.2021.127958

3. The BRCA2 missense mutation K2497R suppressed self-degradation and increased ATP production and cell proliferation

Author:
Gerelmaa Enkhbat; Akira Nakanishi; Yoshio Miki
Source:
Biochemical and biophysical research communications 2022 v.590 pp. 27-33
ISSN:
0006-291X
Subject:
breast neoplasms; cell proliferation; centrosomes; cycloheximide; cytokinesis; genes; heat shock proteins; interphase; lysine; missense mutation; research; tumor suppressor proteins; ubiquitination
Abstract:
... Breast cancer susceptibility gene 2 (BRCA2) mediates genome maintenance during the S phase of the cell cycle, with important roles in replication stress, centrosome replication, and cytokinesis. In this study, we showed that a small heat shock protein, HSP27, interacted with and participated in the degradation of BRCA2 in estrogen-treated MCF-7 cells. BRCA2 degradation reportedly requires ubiquiti ...
DOI:
10.1016/j.bbrc.2021.12.073

4. 1 Effect of cytokine-supplemented maturation medium on bovine somatic cell nuclear transfer embryo development

Author:
J. Keim; Y. Liu; M. Regouski; R. Stott; G. N. Singina; I. A. Polejaeva
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 108
ISSN:
1031-3613
Subject:
blastocyst; bovine serum albumin; calcium; cattle; cycloheximide; embryo transfer; embryogenesis; estrus; fetal bovine serum; fibroblast growth factor 2; fibroblasts; humans; insulin-like growth factor I; leukemia inhibitory factor; magnesium; metaphase; oocytes; penicillins; pregnancy rate; somatic cells; sorbitol; streptomycin
Abstract:
... Invitro maturation is an important process in the invitro production of embryos. It has been shown recently that 3 cytokines: fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF1), increase the efficiency of IVM, blastocyst production, and invivo development following somatic cell nuclear transfer (SCNT) in pigs (Yuan et al. 2017 PNAS 114, E57 ...
DOI:
10.1071/RDv33n2Ab1

5. 133 Effect of cytokines on the quality bovine oocytes matured invitro

Author:
G. N. Singina; E. N. Shedova; T. E. Taradajnic
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 174-175
ISSN:
1031-3613
Subject:
blastocyst; cattle; cell cleavage; cumulus oophorus; cycloheximide; embryo culture; fetal bovine serum; fibroblast growth factor 2; follicle-stimulating hormone; in vitro fertilization; insulin-like growth factor I; ionophores; leukemia inhibitory factor; luteinizing hormone; oocytes; parthenogenesis; pyridines; sodium pyruvate
Abstract:
... As has been shown previously, fibroblast growth factor 2 (FGF2), leukaemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF1) in combination play a positive role in maintaining the quality of mammalian oocytes maturing invitro. In the present work, we studied the effects of these cytokines in optimal concentrations when added in combination to IVM medium on the nuclear status and dev ...
DOI:
10.1071/RDv33n2Ab133

6. Assessing the efficacy of antibiotic treatment to produce earthworms with a suppressed microbiome

Author:
Henny O. Omosigho; Elmer Swart; Tom P. Sizmur; Dave J. Spurgeon; Claus Svendsen; Liz J. Shaw
Source:
European journal of soil biology 2022 v.108 pp. 103366
ISSN:
1164-5563
Subject:
Aeromonas; Allolobophora chlorotica; Comamonas; Eisenia fetida; Lumbricus terrestris; Sphingobacterium; agar; ampicillin; ciprofloxacin; community structure; culture media; cycloheximide; earthworms; gentamicin; microbiome; nalidixic acid; organic matter; sequence analysis; soil
Abstract:
... Earthworms are an integral part of soil ecosystems, especially for their role in soil functions such as organic matter (OM) decomposition and nutrient cycling. Earthworms and microorganisms are interdependent, and a considerable portion of the contribution earthworms make to influencing OM fate is through interactions with microorganisms. However, the importance of the earthworm-associated microbi ...
DOI:
10.1016/j.ejsobi.2021.103366

7. Danhe granule ameliorates nonalcoholic steatohepatitis and fibrosis in rats by inhibiting ceramide de novo synthesis related to CerS6 and CerK

Author:
Meixia Zhu; Zhixin Jia; Xiaoning Yan; Lirong Liu; Cong Fang; Menghan Feng; Yihang Dai; Yinhuan Zhang; Hao Wu; Beibei Huang; Yueting Li; Jie Liu; Hongbin Xiao
Source:
Journal of ethnopharmacology 2022 v.295 pp. 115427
ISSN:
0378-8741
Subject:
animal models; blood serum; blood stasis; body weight; ceramide glucosyltransferase; ceramides; cycloheximide; diet; enzyme activity; fatty liver; fibrosis; free fatty acids; inflammation; interleukin-6; liver; mechanism of action; non-specific serine/threonine protein kinase; oils; protein synthesis; quantitative polymerase chain reaction; serine; sphingosine N-acyltransferase; traditional medicine; triacylglycerols
Abstract:
... Danhe granule (DHG) is used by Chinese doctors to treat blood stasis, phlegm and dampness. Its lipid-lowering ability has been investigated in our previous research. However, the anti-liver inflammatory and fibrotic effects and mechanism of action of DHG in non-alcoholic steatohepatitis (NASH) have not been explored. To evaluate the ameliorative effects of DHG on liver inflammation and fibrosis in ...
DOI:
10.1016/j.jep.2022.115427

8. Enhanced GRP78 protein expression via the IRE1α/ASK1/p38 MAPK pathway during As2O3-induced endoplasmic reticulum stress in BEAS-2B cells

Author:
Jiaming Yuan; Chenjuan Yao; Jing Tang; Yingqi Liu; Chunyan Huang; Shali Yu; Haiyan Wei; Yu Han; Gang Chen
Source:
Toxicology 2021 v.462 pp. 152962
ISSN:
0300-483X
Subject:
actinomycin D; air; apoptosis; arsenic; arsenic oxide; biosynthesis; cell viability; cycloheximide; endoplasmic reticulum; endoplasmic reticulum stress; gene expression; mitogen-activated protein kinase; models; phosphorylation; protein synthesis inhibitors; ribonucleases; toxicology; unfolded protein response
Abstract:
... Inorganic arsenic is widely present in the environment. Exposure to moderate to high concentrations of arsenic from drinking water or air can cause various cancers and multisystem dysfunction. Glucose-regulated protein 78 (GRP78) is an endoplasmic reticulum (ER) stress sensor of unfolded protein response (UPR) under stress conditions and it enhances cell survival. The aim of this study is to inves ...
DOI:
10.1016/j.tox.2021.152962

9. FBX4 mediates rapid cyclin D1 proteolysis upon DNA damage in immortalized esophageal epithelial cells

Author:
Jia Liu; Hui Yang; Pak Yan Cheung; Sai Wah Tsao; Liang Lv; Annie L.M Cheung
Source:
Biochemical and biophysical research communications 2021 v.554 pp. 76-82
ISSN:
0006-291X
Subject:
DNA; DNA damage; carcinogenesis; cell cycle checkpoints; cyclins; cycloheximide; epithelium; esophageal neoplasms; genetic instability; irradiation; proteolysis; research; squamous cell carcinoma; ubiquitin-protein ligase
Abstract:
... It has been implied that deregulation of cyclin D1 turnover under stresses can facilitate genomic instability and trigger tumorigenesis. Much focus has been placed on identifying the E3 ligases responsible for mediating cyclin D1 degradation. However, the findings were quite controversial and cell type-dependent. Little is known about how cyclin D1 is regulated in precancerous cells upon DNA damag ...
DOI:
10.1016/j.bbrc.2021.03.089

10. Halofuginone triggers a transcriptional program centered on ribosome biogenesis and function in honey bees

Author:
Melissa E. Flores; Nora K. McNamara-Bordewick; Natalie L. Lovinger; Jonathan W. Snow
Source:
Insect biochemistry and molecular biology 2021 v.139 pp. 103667
ISSN:
0965-1748
Subject:
Nosema ceranae; amino acids; biogenesis; cycloheximide; gene expression; halofuginone; honey; insect biochemistry; molecular biology; pollinators; quality control; ribosomes; stress response; therapeutics; toxicity; transcription (genetics); unfolded protein response
Abstract:
... We previously found that pharmacological inhibition of prolyl-tRNA synthetase by halofuginone has potent activity against Nosema ceranae, an important pathogen of honey bees. However, we also observed that prolyl-tRNA synthetase inhibition is toxic to bees, suggesting further work is necessary to make this a feasible therapeutic strategy. As expected, we found that pharmacological inhibition of pr ...
DOI:
10.1016/j.ibmb.2021.103667

11. Identification and utilization of a mutated 60S ribosomal subunit coding gene as an effective and cost-efficient selection marker for Tetrahymena genetic manipulation

Author:
Yu Qiao; Ting Cheng; Jiachen Zhang; Saleh A. Alfarraj; Miao Tian; Yifan Liu; Shan Gao
Source:
International journal of biological macromolecules 2022 v.204 pp. 1-8
ISSN:
0141-8130
Subject:
Tetrahymena thermophila; cost effectiveness; cycloheximide; eukaryotic cells; genetic engineering; molecular biology; resistance genes
Abstract:
... Since the onset of molecular biology, the ciliate Tetrahymena thermophila has been one of the most convenient single-celled model eukaryotes for genetics, biochemistry, and cell biology. Particularly, thanks to the availability of several different selection markers, it is possible to knock out or knock in genes at multiple genetic loci simultaneously in Tetrahymena, which makes it an excellent mo ...
DOI:
10.1016/j.ijbiomac.2022.01.188

12. In vitro induction of catfish, Clarias batrachus, oocyte maturation by conspecific vitellogenin 1 (CFVg1)

Author:
Debapriya Bhattacharya; Shrabanti Sarkar; Panchanan Nath
Source:
Fish physiology and biochemistry 2022 v.48 no.1 pp. 227-239
ISSN:
0920-1742
Subject:
Clarias batrachus; actinomycin D; appetite; carp; catfish; conspecificity; cycloheximide; oocytes; theophylline; trypsin; vitellogenin
Abstract:
... Present study demonstrates that conspecific vitellogenin1 (CFVg1) induces oocyte maturation in the catfish, Clarias batrachus. CFVg1 is able to develop fertilizable eggs in the Clarias batrachus. Therefore, different in vitro oocyte culture experiments were designed to see whether CFVg1 has efficacy of oocyte maturation and its pathway. In in vitro oocyte culture experiment, CFVg1 showed a dose- a ...
DOI:
10.1007/s10695-022-01050-y

13. Insulin- and cholic acid-loaded zein/casein–dextran nanoparticles enhance the oral absorption and hypoglycemic effect of insulin

Author:
Xiaoyan Bao; Kang Qian; Ping Yao
Source:
Journal of materials chemistry B 2021 v.9 no.31 pp. 6234-6245
ISSN:
2050-7518
Subject:
Maillard reaction; absorption; bioavailability; blood; casein; cement; cholic acid; cycloheximide; dextran; diabetes mellitus; glycemic effect; hydrodynamics; hydrolysis; hydrophilicity; hydrophobicity; ileum; insulin; liver; permeability; proteinases; zein
Abstract:
... Diabetes mellitus is the most common metabolic disease in the world. Herein, insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces were fabricated to enhance the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin. In the nanoparticles, zein acted as cement to embed insulin, cholic acid and casein by hydrophobic i ...
DOI:
10.1039/d1tb00806d

14. Persistent Effect of Advanced Glycated Albumin Driving Inflammation and Disturbances in Cholesterol Efflux in Macrophages

Author:
Carlos André Minanni; Adriana Machado-Lima; Rodrigo Tallada Iborra; Lígia Shimabukuro Okuda; Raphael de Souza Pinto; Monique de Fátima Mello Santana; Aécio Lopes de Araújo Lira; Edna Regina Nakandakare; Maria Lúcia Cardillo Côrrea-Giannella; Marisa Passarelli
Source:
Nutrients 2021 v.13 no.10 pp. -
ISSN:
2072-6643
Subject:
albumins; blood serum; cholesterol; cholesterol homeostasis; cycloheximide; cytokines; diabetes mellitus; glycemic control; homeostasis; inflammation; lipid content; macrophages; protein content; secretion
Abstract:
... Advanced glycated albumin (AGE-albumin) impairs cholesterol efflux and contributes to inflammation in macrophages. The current study evaluated: (1) the persistence of the deleterious effect of AGE-albumin in cholesterol efflux and in inflammation, and (2) how metabolic control in diabetes mellitus (DM) contributes to attenuate the deleterious role of AGE-albumin in macrophage cholesterol homeostas ...
DOI:
10.3390/nu13103633

15. Relative contributions of bacteria and fungi to nitrous oxide emissions following nitrate application in soils representing different land uses

Author:
Antonio Castellano-Hinojosa; Kate Le Cocq; Alice F. Charteris; Maider Abadie; David R. Chadwick; Ian M. Clark; Jesús González-López; Eulogio J. Bedmar; Laura M. Cardenas
Source:
International biodeterioration & biodegradation 2021 v.159 pp. 105199
ISSN:
0964-8305
Subject:
biodegradation; carbon dioxide; cycloheximide; denitrification; denitrifying microorganisms; fungi; fungicides; gene dosage; genes; greenhouses; internal transcribed spacers; nitrous oxide; pollution control; quantitative polymerase chain reaction; soil treatment; soil types; streptomycin
Abstract:
... Bacteria and fungi have been shown to produce nitrous oxide (N₂O) during denitrification, but their contribution after nitrate (NO₃⁻) application to soil is not clearly established. In a microcosm experiment, the relative contribution of bacteria and fungi to N₂O and carbon dioxide (CO₂) production by four contrasting soils representing different land uses after KNO₃ addition was studied. The soil ...
DOI:
10.1016/j.ibiod.2021.105199

16. STUB1/CHIP promotes ubiquitination and degradation of HIV-1 Vif to restore the cellular level of APOBEC3G protein

Author:
Amjad Ali; Vivek Kumar; Akhil C. Banerjea
Source:
Biochemical and biophysical research communications 2021 v.574 pp. 27-32
ISSN:
0006-291X
Subject:
cycloheximide; dose response; genomics; neutralization; research; ubiquitin-protein ligase; ubiquitination
Abstract:
... HIV-1 accessory protein Vif is required for neutralization of cellular restriction factor APOBEC3G through its ubiquitination and proteasomal degradation which allows replication of HIV-1 in non-permissive cells. This function of Vif is required for maintaining the genomic integrity of HIV-1. We here report that the Vif interacts with the cellular E3 ubiquitin ligase CHIP and the level of Vif prot ...
DOI:
10.1016/j.bbrc.2021.08.031

17. Synergistic Interferon-Alpha-Based Combinations for Treatment of SARS-CoV-2 and Other Viral Infections

Author:
Aleksandr Ianevski; Rouan Yao; Eva Zusinaite; Laura Sandra Lello; Sainan Wang; Eunji Jo; Jaewon Yang; Erlend Ravlo; Wei Wang; Hilde Lysvand; Kirsti Løseth; Valentyn Oksenych; Tanel Tenson; Marc P. Windisch; Minna M. Poranen; Anni I. Nieminen; Svein Arne Nordbø; Mona Høysæter Fenstad; Gunnveig Grødeland; Pål Aukrust; Marius Trøseid; Anu Kantele; Eglė Lastauskienė; Astra Vitkauskienė; Nicolas Legrand; Andres Merits; Magnar Bjørås; Denis E. Kainov
Source:
Viruses 2021 v.13 no.12 pp. -
ISSN:
1999-4915
Subject:
RNA; Severe acute respiratory syndrome coronavirus 2; antiviral agents; blood serum; cycloheximide; homeostasis; lamivudine; lungs; metabolomics; organoids; protein synthesis; therapeutics; transcriptome
Abstract:
... Background: There is an urgent need for new antivirals with powerful therapeutic potential and tolerable side effects. Methods: Here, we tested the antiviral properties of interferons (IFNs), alone and with other drugs in vitro. Results: While IFNs alone were insufficient to completely abolish replication of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), IFNα, in combination with re ...
DOI:
10.3390/v13122489

18. The E3 ubiquitin ligase SCF(Fbxo7) mediates proteasomal degradation of UXT isoform 2 (UXT-V2) to inhibit the NF-κB signaling pathway

Author:
Valentine Spagnol; Caio A.B. Oliveira; Suzanne J. Randle; Patrícia M.S. Passos; Camila R.S.T.B. Correia; Natália B. Simaroli; Joice S. Oliveira; Tycho E.T. Mevissen; Ana Carla Medeiros; Marcelo D. Gomes; David Komander; Heike Laman; Felipe Roberti Teixeira
Source:
Biochimica et biophysica acta 2021 v.1865 no.1 pp. 129754
ISSN:
0304-4165
Subject:
F-box proteins; alternative splicing; androgens; apoptosis; cycloheximide; estrogens; microscopy; precipitin tests; transcription (genetics); ubiquitin; ubiquitin-protein ligase; ubiquitination
Abstract:
... Ubiquitously eXpressed Transcript isoform 2 (UXTV2) is a prefoldin-like protein involved in NF-κB signaling, apoptosis, and the androgen and estrogen response. UXT-V2 is a cofactor in the NF-κB transcriptional enhanceosome, and its knockdown inhibits TNF-α -induced NF-κB activation. Fbxo7 is an F-box protein that interacts with SKP1, Cullin1 and RBX1 proteins to form an SCF(Fbxo7) E3 ubiquitin lig ...
DOI:
10.1016/j.bbagen.2020.129754

19. The effect of soil moisture on the response by fungi and bacteria to nitrogen additions for N2O production

Author:
Lei Zhang; Junqiang Zheng; Xu Han; Junhui Zhang; Chengxu Li; Shicong Geng; Shijie Han
Source:
Journal of forestry research 2021 v.32 no.5 pp. 2037-2045
ISSN:
1007-662X
Subject:
carbon dioxide; cycloheximide; forestry; fungi; microbial activity; nitrogen; nitrous oxide; nitrous oxide production; research; soil water; streptomycin; temperate forests; China
Abstract:
... In addition to bacteria, the contribution of fungi to nitrous oxide (N₂O) production has been recognized but the responses of these two broad and unrelated groups of microorganisms to global environmental changes, atmospheric nitrogen (N) deposition, and precipitation in terms of N₂O production are unclear. We studied how these two microbial-mediated N₂O production pathways responded to soil moist ...
DOI:
10.1007/s11676-020-01262-z

20. The foodborne contaminant deoxynivalenol exacerbates DNA damage caused by a broad spectrum of genotoxic agents

Author:
Marion Garofalo; Delphine Payros; Eric Oswald; Jean-Philippe Nougayrède; Isabelle P. Oswald
Source:
Science of the total environment 2022 v.820 pp. 153280
ISSN:
0048-9697
Subject:
DNA damage; cisplatin; cycloheximide; deoxynivalenol; digestive system; environment; etoposide; food chain; genotoxicity; mutagens; phenotype; ribosomes
Abstract:
... Humans are exposed to different contaminants including mycotoxins. Deoxynivalenol (DON), a potent ribosome inhibitor, is a highly prevalent mycotoxin in the food chain worldwide. Although DON is not genotoxic, we previously showed that it exacerbates the genotoxicity of colibactin, a DNA-crosslinking toxin produced by bacteria in the gut. In the present study, we investigated whether this phenotyp ...
DOI:
10.1016/j.scitotenv.2022.153280