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1. Arabidopsis PLDζ1 and PLDζ2 localize to post-Golgi membrane compartments in a partially overlapping manner

Author:
Ryota Shimamura; Yohei Ohashi; Yukimi Yamamoto Taniguchi; Mariko Kato; Tomohiko Tsuge; Takashi Aoyama
Source:
Plant molecular biology 2022 v.108 no.1-2 pp. 31-49
ISSN:
0167-4412
Subject:
Arabidopsis thaliana; cytoskeleton; dissection; fluorescence; lateral roots; moieties; molecular biology; phosphatidic acids; phospholipases; root cap; signal transduction; tonoplast
Abstract:
... KEY MESSAGE: Arabidopsis PLDζ1 and PLDζ2 localize to the trans-Golgi network and to compartments including the trans-Golgi network, multi-vesicular bodies, and the tonoplast, respectively, depending on their N-terminal regions containing PX-PH domains. Phospholipase D (PLD) is involved in dynamic cellular processes, including membrane trafficking, cytoskeletal reorganization, and signal transducti ...
DOI:
10.1007/s11103-021-01205-0

2. Bidirectional regulation of desmosome hyperadhesion by keratin isotypes and desmosomal components

Author:
Fanny Büchau; Franziska Vielmuth; Jens Waschke; Thomas M. Magin
Source:
Cellular and molecular life sciences 2022 v.79 no.5 pp. 223
ISSN:
1420-682X
Subject:
atomic force microscopy; cohesion; cytoskeleton; desmosomes; image analysis; keratin; keratinocytes; mechanical stress; pathogenesis; plasma membrane; tissue repair
Abstract:
... Desmosomes are intercellular junctions which mediate cohesion and communication in tissues exposed to mechanical strain by tethering the intermediate filament cytoskeleton to the plasma membrane. While mature desmosomes are characterized by a hyperadhesive, Ca²⁺-independent state, they transiently loose this state during wound healing, pathogenesis and tissue regeneration. The mechanisms controlli ...
DOI:
10.1007/s00018-022-04244-y

3. Biochemical Properties of Two Plasmodium malariae Cysteine Proteases, Malapain-2 and Malapain-4

Author:
Hương Giang Lê; Jung-Mi Kang; Tuấn Cường Võ; Won Gi Yoo; Kon Ho Lee; Byoung-Kuk Na
Source:
Microorganisms 2022 v.10 no.1 pp. -
ISSN:
2076-2607
Subject:
Plasmodium malariae; antimalarials; arginine; cysteine proteinases; cytoskeleton; enzyme activity; erythrocytes; hemoglobin; humans; hydrolysis; leucine; malaria; merozoites; pH; phenylalanine; phylogeny; spectrin
Abstract:
... Cysteine proteases belonging to the falcipain (FP) family play a pivotal role in the biology of malaria parasites and have been extensively investigated as potential antimalarial drug targets. Three paralogous FP-family cysteine proteases of Plasmodium malariae, termed malapains 2–4 (MP2–4), were identified in PlasmoDB. The three MPs share similar structural properties with the FP-2/FP-3 subfamily ...
DOI:
10.3390/microorganisms10010193

4. Camellia sinensis small GTPase gene (CsRAC1) involves in response to salt stress, drought stress and ABA signaling pathway

Author:
Xiaohan Xu; Xiaoli Ye; Anqi Xing; Zichen Wu; Xuyan Li; Zaifa Shu; Yuhua Wang
Source:
Gene 2022 v.821 pp. 146318
ISSN:
0378-1119
Subject:
Arabidopsis thaliana; Camellia sinensis; abscisic acid; antioxidants; cell membranes; cold; cytoskeleton; drought; genes; guanosinetriphosphatase; heat; physiological transport; salt stress; seed germination; tea; tobacco; water stress
Abstract:
... RAC/ROP gene (RACs) is a plant-specific small GTPases. RACs play an irreplaceable role in the tissue dynamics of cytoskeleton, vesicle transport and hormone signal transmission in plants. In the present study, a novel gene from RACs family, CsRAC1, was identified from tea [Camellia sinensis (L.) O. Kuntze]. CsRAC1 contained a 591-bp open reading frame and encoded a putative protein of 197 amino ac ...
DOI:
10.1016/j.gene.2022.146318

5. Chitosan‑sodium alginate-collagen/gelatin three-dimensional edible scaffolds for building a structured model for cell cultured meat

Author:
Linzi Li; Lin Chen; Xiaohong Chen; Yan Chen; Shijie Ding; Xiaojing Fan; Yaping Liu; Xinglian Xu; Guanghong Zhou; Beiwei Zhu; Niamat Ullah; Xianchao Feng
Source:
International journal of biological macromolecules 2022 v.209 pp. 668-679
ISSN:
0141-8130
Subject:
adhesion; biocompatibility; chewiness; chitosan; cytoskeleton; extracellular matrix; fluorescent antibody technique; freeze drying; models; pork; skeletal muscle; sodium alginate; swine; texture
Abstract:
... Cell cultured meat (CCM) production is an innovative technology that does not depend on livestock farming practices to produce meat. The construction of structured CCM requires a three-dimensional (3D) scaffold to mimic the extracellular matrix to provide mechanical support for the cells. Furthermore, the 3D scaffolds should be edible and have good biocompatibility and tissue-like texture. Here, w ...
DOI:
10.1016/j.ijbiomac.2022.04.052

6. Chronic microglial inflammation promotes neuronal lactate supply but impairs its utilization in primary rat astrocyte-neuron co-cultures

Author:
Yi Wang; Jie Li; Meng-Yue Wang; Zhi-Yong Pan; Zhi-Qiang Li; Ze-Fen Wang
Source:
Biochemical and biophysical research communications 2022 v.607 pp. 28-35
ISSN:
0006-291X
Subject:
adenosine triphosphate; astrocytes; coculture; cytoskeleton; energy; energy metabolism; glucose; inflammation; lactic acid; lipopolysaccharides; neurons; neurotoxicity; oxidation; rats; research
Abstract:
... Neuronal activity is closely associated with energy metabolism. In addition to glucose, astrocyte-derived lactate serves as an energy source for neurons. Chronic inflammation is a common pathological event that is associated with aging and neurodegenerative diseases. However, the mechanisms underlying inflammation-induced neuronal injury are not fully understood. Both microglia and astrocytes part ...
DOI:
10.1016/j.bbrc.2022.03.122

7. Comparative transcriptome analyses unravel the response to acute thermal stress in the razor clam, Sinonovacula constricta

Author:
Xianghui Kong; Liyuan Lv; Jianfeng Ren; Youli Liu; Zhihua Lin; Yinghui Dong
Source:
Aquaculture reports 2022 v.23 pp. 101079
ISSN:
2352-5134
Subject:
Veneroida; allografting; antigens; antioxidant activity; aquaculture; cytoskeleton; endoplasmic reticulum; evolution; fibrillins; gene expression regulation; heat stress; heat tolerance; hepatopancreas; homeostasis; immune response; lectins; lipid metabolism; transcriptome; transcriptomics
Abstract:
... The razor clam, Sinonovacula constricta, must have acquired adaptive mechanisms to defense thermal pressure during evolution as it’s an eurythermal bivalve. However, the specific mechanisms remain unclear. To identify thermal responsive genes and unravel adaptive mechanisms, the clams were subjected to acute thermal stress from 21 °C to 32 °C for 24 h. The transcriptomic profilings of gills and he ...
DOI:
10.1016/j.aqrep.2022.101079

8. Comparative transcriptome profiling reveals the basis of differential sheath blight disease response in tolerant and susceptible rice genotypes

Author:
Pankajini Samal; Kutubuddin A. Molla; Archana Bal; Soham Ray; Harekrushna Swain; Ansuman Khandual; Pritiranjan Sahoo; Motilal Behera; Sarika Jaiswal; Asif Iquebal; Mridul Chakraborti; Lambodar Behera; Meera K. Kar; Arup K. Mukherjee
Source:
Protoplasma 2022 v.259 no.1 pp. 61-73
ISSN:
0033-183X
Subject:
Thanatephorus cucumeris; biosynthesis; cytoskeleton; fungi; germplasm; glycolysis; glycosidases; host-pathogen relationships; leaf blight; pathogens; photosynthesis; rice; secondary metabolites; transcriptome
Abstract:
... Rice sheath blight (ShB) disease, caused by the fungal pathogen Rhizoctonia solani AG1-IA, is one of the devastating diseases and causes severe yield losses all over the world. No completely resistant germplasm is known till now, and as a result, the progress in resistance breeding is unsatisfactory. Basic studies to identify candidate genes, QTLs, and to better understand the host–pathogen intera ...
DOI:
10.1007/s00709-021-01637-x

9. Cytoskeletal Alteration Is an Early Cellular Response in Pulmonary Epithelium Infected with Aspergillus fumigatus Rather than Scedosporium apiospermum

Author:
Tapanee Kanjanapruthipong; Passanesh Sukphopetch; Onrapak Reamtong; Duangnate Isarangkul; Watcharamat Muangkaew; Tipparat Thiangtrongjit; Nichapa Sansurin; Kamonpan Fongsodsri; Sumate Ampawong
Source:
Microbial ecology 2022 v.83 no.1 pp. 216-235
ISSN:
0095-3628
Subject:
Aspergillus fumigatus; Scedosporium; amphotericin B; aspergillosis; chitin; cytoskeleton; epithelium; fungi; histopathology; immunoassays; immunohistochemistry; interleukin-12; interleukin-17; interleukin-2; interleukin-8; metalloproteinases; mice; microbial ecology; mortality; necrosis; neoplasms; pathogenesis; proteomics
Abstract:
... Invasive aspergillosis and scedosporiosis are life-threatening fungal infections with similar clinical manifestations in immunocompromised patients. Contrarily, Scedosporium apiospermum is susceptible to some azole derivative but often resistant to amphotericin B. Histopathological examination alone cannot diagnose these two fungal species. Pathogenesis studies could contribute to explore candidat ...
DOI:
10.1007/s00248-021-01750-7

10. Effect of substrate stiffness on the mechanical properties of cervical cancer cells

Author:
Ying Zhuang; Yihong Huang; Zijian He; Tianqi Liu; Xuefei Yu; Sherman Xuegang Xin
Source:
Archives of biochemistry and biophysics 2022 v.725 pp. 109281
ISSN:
0003-9861
Subject:
adhesion; atomic force microscopy; biophysics; cell membranes; collagen; computer software; cytoskeleton; gels; glass; integrins; mechanotransduction; modulus of elasticity; phalloidine; polyacrylamide; uterine cervical neoplasms
Abstract:
... Cervical cancer microenvironment is involved in the regulation of the behavior, morphology, and mechanical properties of the cervical cancer cells. Integrins expressed on the cell membrane combine with the factors of the microenvironment to determine cervical cancer cells’ properties. The mechanical properties of integrin-extracellular matrix (ECM) interactions are important for the mechanotransdu ...
DOI:
10.1016/j.abb.2022.109281

11. Epoxiconazole profoundly alters rat brain and properties of neural stem cells

Author:
Hiba Hamdi; Imen Graiet; Salwa Abid-Essefi; Joel Eyer
Source:
Chemosphere 2022 v.288 pp. 132640
ISSN:
0045-6535
Subject:
DNA fragmentation; acetylcysteine; adults; apoptosis; brain; caspase-3; cell proliferation; comet assay; cytoskeleton; epoxiconazole; histology; inhibitory concentration 50; lipid peroxidation; membrane potential; mitochondria; neonates; neurotoxicity; no observed adverse effect level; oral exposure; oxidation; oxidative stress; rats; toxic substances; triazoles
Abstract:
... Epoxiconazole (EPX), a widely used fungicide for domestic, medical, and industrial applications, could cause neurodegenerative diseases. However, the underling mechanism of neurotoxicity is not well understood. This study aimed to investigate the possible toxic outcomes of Epoxiconzole, a triazole fungicide, on the brain of adult rats in vivo, and in vitro on neural stem cells derived from the sub ...
DOI:
10.1016/j.chemosphere.2021.132640

12. Genome-wide association study (GWAS) of growth traits in olive flounder (Paralichthys olivaceus)

Author:
W.K.M. Omeka; D.S. Liyanage; Sukkyoung Lee; Chaehyeon Lim; Hyerim Yang; W.M. Gayashani Sandamalika; H.M.V. Udayantha; Gaeun Kim; Subothini Ganeshalingam; Taehyug Jeong; Seong-Rip Oh; Seung-Hwan Won; Hyoung-Bum Koh; Mun-Kwan Kim; David B. Jones; Cecile Massault; Dean R. Jerry; Jehee Lee
Source:
Aquaculture 2022 v.555 pp. 738257
ISSN:
0044-8486
Subject:
Paralichthys olivaceus; body weight; cytoskeleton; farmed fish; females; flounder; gene ontology; genome-wide association study; genomics; genotyping; growth performance; males; marker-assisted selection; market value; neurons; phenotypic correlation; profitability; quality control; South Korea
Abstract:
... Traits linked to growth performance in farmed fish are major drivers of profitability in aquaculture systems. Given the importance of growth-related traits, there have been efforts to unravel the genomic architecture of growth traits and to identify key genes or genomic regions useful as selection markers in various species. Such markers would increase the accuracy of selection and improve rates o ...
DOI:
10.1016/j.aquaculture.2022.738257

13. Genome-wide identification, characterization, and expression analysis of keratin genes (KRTs) family in yak (Bos grunniens)

Author:
Qi Bao; Xiaolan Zhang; Pengjia Bao; Chunnian Liang; Xian Guo; Mancai Yin; Min Chu; Ping Yan
Source:
Gene 2022 v.818 pp. 146247
ISSN:
0378-1119
Subject:
bioinformatics; cattle; cell cycle; cytoskeleton; gene expression; genes; germplasm conservation; hair follicles; humans; keratin; keratinocytes; melanogenesis; mice; phosphorylation; phylogeny; rats; sheep; transcriptome; yaks
Abstract:
... As the largest subgroup of intermediate filament proteins, keratins are divided into two types of subfamily. Currently, the molecular mechanism of keratins in several animals has been reported but is limited in yak. Here, 53 different kinds of keratins were identified in the yak genome, including 23 type I and 30 type II keratins. Bioinformatics analysis in this study revealed that multiple phosph ...
DOI:
10.1016/j.gene.2022.146247

14. Genotoxic effects of electromagnetic field radiations from mobile phones

Author:
Ganesh Chandra Jagetia
Source:
Environmental research 2022 pp. 113321
ISSN:
0013-9351
Subject:
DNA adducts; Internet; chest; chromosomes; cytoskeleton; electric power; electromagnetic field; headache; humans; immunity; memory disorders; metabolism; mutagens; nausea; radio waves; reactive oxygen species; research; signal transduction; skin irritation; sleep; television; therapeutics; wireless technology
Abstract:
... The use of wireless communication technology in mobile phones has revolutionized modern telecommunication and mobile phones have become so popular that their number exceeds the global population. Electromagnetic field radiations (EMR) are an integral part of wireless technology, which are emitted by mobile phones, mobile towers, electric power stations, transmission lines, radars, microwave ovens, ...
DOI:
10.1016/j.envres.2022.113321

15. In vitro effects of the 4-[(10H-phenothiazin-10-yl)methyl]-N-hydroxybenzamide on Giardia intestinalis trophozoites

Author:
Roberta Veríssimo F. Oliveira; Wanderley de Souza; Katharina Vögerl; Franz Bracher; Marlene Benchimol; Ana Paula R. Gadelha
Source:
Acta tropica 2022 v.232 pp. 106484
ISSN:
0001-706X
Subject:
Giardia lamblia; benzimidazoles; cell membranes; cytokinesis; cytoskeleton; drug therapy; drugs; genome; giardiasis; glycogen; histones; intestines; lysine; nitroimidazoles; parasites; trophozoites; tubulin; ultrastructure; viability
Abstract:
... Giardiasis is an intestinal disease caused by the parasite protozoan Giardia intestinalis. For more than five decades, the treatment of this disease has been based on compounds such as nitroimidazoles and benzimidazoles. The parasite's adverse effects and therapeutic failure are largely recognized. Therefore, it is necessary to develop new forms of chemotherapy treatment against giardiasis. Lysine ...
DOI:
10.1016/j.actatropica.2022.106484

16. Interaction of influenza A virus NS1 and cytoskeleton scaffolding protein α-actinin 4

Author:
Yingyue Zeng; Rui Wang; Fengchao Wang; Man Zhang; Li Zhang; Chunyu Zhu; Jian Zhao; Hongsheng Liu
Source:
Virus genes 2022 v.58 no.1 pp. 15-22
ISSN:
0920-8569
Subject:
Influenza A virus; absorbance; cytoskeleton; drugs; enzyme-linked immunosorbent assay; fluorescent antibody technique; glutathione transferase; humans; lungs; mass spectrometry; neoplasm cells; pathogenesis; precipitin tests; viral nonstructural proteins; virulence; viruses
Abstract:
... NS1 (Non-structural protein 1) is a non-structural protein that can highly express when the avian influenza virus infects the host cells. NS1 can interact with various proteins to alter the intracellular distribution of host proteins and regulate the virulence and pathogenicity of the avian influenza virus. To further study the role of NS1 protein in replication and pathogenesis of avian influenza ...
DOI:
10.1007/s11262-021-01876-3

17. Involvement of the fusome-related protein Add1 in spermatogenesis of the Chinese mitten crab (Eriocheir sinensis) by organization of cytoskeleton

Author:
Lishuang Sun; Anni Ni; Yulian Tang; Shu Li; Genliang Li
Source:
Aquaculture international 2022 v.30 no.2 pp. 721-733
ISSN:
0967-6120
Subject:
Eriocheir sinensis; Western blotting; aquaculture; chromatin; cytoskeleton; fluorescent antibody technique; skeleton; spermatids; spermatocytes; spermatogonia; spermatozoa; spermiogenesis; transcription (genetics)
Abstract:
... The sperm nucleus of the decapod crustacean Chinese mitten crab, Eriocheir sinensis, is loose and fibrous, which is a non-condensed nucleus. Our purpose was to analyze the structural distribution of the fusome-related protein Add1 during the spermatogenesis in E. sinensis to better understand the non-condensed nucleus of E. sinensis. RT-qPCR and western blot results showed that Add1 was expressed ...
DOI:
10.1007/s10499-021-00825-z

18. Is Cryptosporidium a hijacker able to drive cancer cell proliferation?

Author:
Gabriela Certad
Source:
Food and Waterborne Parasitology 2022 v.27 pp. e00153
ISSN:
2405-6766
Subject:
Cryptosporidium muris; actin; adenocarcinoma; adults; animal models; carcinogenesis; cell proliferation; colon; colorectal neoplasms; cryptosporidiosis; cytoskeleton; epithelium; guanosinetriphosphatase; humans; microarray technology; neoplasm cells; oocysts; parasites; parasitology; phosphatidylinositol 3-kinase; severe combined immunodeficiency; signal transduction
Abstract:
... The pathophysiological mechanisms of Cryptosporidium infection are multifactorial and not completely understood. Some advances achieved recently revealed that the infection by Cryptosporidium parvum induces cytoskeleton remodeling and actin reorganization through the implication of several intracellular signals involving, for example, PI3K, Src, Cdc42 and GTPases. It has also been reported that th ...
DOI:
10.1016/j.fawpar.2022.e00153

19. Label-free based proteomics revealed the specific changes of muscle proteins in pike eel (Muraenesox cinereus) under cold stress

Author:
Pengxiang Yuan; Xiaonan Chen; Soottawat Benjakul; Jipeng Sun; Bin Zhang
Source:
Food chemistry: X 2022 v.14 pp. 100275
ISSN:
2590-1575
Subject:
Muraenesox cinereus; cold; cold stress; cytoskeleton; fibronectins; food chemistry; frozen storage; histones; liquid chromatography; mass spectrometry; muscles; proteomics; tubulin
Abstract:
... Chemical- and liquid chromatography coupled with mass spectrometry (LC–MS) based proteomics strategies were executed to investigate the alterations of protein profiles in pike eel (Muraenesox cinereus) muscle during chilling (CPE) and frozen (FPE) storage. Chemical results indicated that springiness and myofibrillar protein (MP) content of muscle tissues decreased significantly during 6 days of ch ...
DOI:
10.1016/j.fochx.2022.100275

20. Light-based 3D bioprinting of bone tissue scaffolds with tunable mechanical properties and architecture from photocurable silk fibroin

Author:
Monika Rajput; Pritiranjan Mondal; Parul Yadav; Kaushik Chatterjee
Source:
International journal of biological macromolecules 2022 v.202 pp. 644-656
ISSN:
0141-8130
Subject:
biodegradability; biomechanics; bioprinting; bone formation; bones; calcium; cell proliferation; cytoskeleton; fibroins; humans; hydrogels; silk; three-dimensional printing; viability; viscoelasticity
Abstract:
... Three-dimensional (3D) bioprinting based on digital light processing (DLP) offers unique opportunities to prepare scaffolds that mimic the architecture and biomechanical properties of human tissues. Limited availability of biocompatible and biodegradable bioinks amenable for DLP-bioprinting is an impediment in this field. This study presents a bioink prepared from silk fibroin (SF) tailored for DL ...
DOI:
10.1016/j.ijbiomac.2022.01.081

21. Molecular characterization of a profilin gene from a parasitic ciliate Cryptocaryon irritans

Author:
Zhongfeng Ye; Wei Ni; Jingwei Zhang; Yinan Zhang; Lingying Yu; Xiaohong Huang
Source:
Experimental parasitology 2022 v.236-237 pp. 108248
ISSN:
0014-4894
Subject:
Cryptocaryon irritans; Escherichia coli; Western blotting; actin; agarose; cDNA libraries; cell membranes; cell movement; codons; coprecipitation; cytokinesis; cytoskeleton; gene expression; glutathione; glutathione transferase; molecular weight; neurons; neuroplasticity; parasitology; plasmids; polyacrylamide gel electrophoresis; polypeptides; profilins; quantitative polymerase chain reaction; site-directed mutagenesis; thrombin
Abstract:
... Profilin, known as one of the core actin-binding proteins, is an integral part of actin-based cytoskeleton involved in cell motility, cytokinesis, neuronal differentiation, and synaptic plasticity. In this study, a putative profilin gene designated as CiProfilin (GenBank accession number: JX987286) was screened out from a cDNA library of Cryptocaryon irritans trophonts. The full-length cDNA of CiP ...
DOI:
10.1016/j.exppara.2022.108248

22. Plant Low-Temperature Stress: Signaling and Response

Author:
Mohammad Aslam; Beenish Fakher; Mohammad Arif Ashraf; Yan Cheng; Bingrui Wang; Yuan Qin
Source:
Agronomy 2022 v.12 no.3 pp. -
ISSN:
2073-4395
Subject:
Arabidopsis; acclimation; agronomy; calcium; cold; cold shock response; cold stress; crop yield; cytoskeleton; epigenetics; mitogen-activated protein kinase; plant development; rice
Abstract:
... Cold stress has always been a significant limitation for plant development and causes substantial decreases in crop yield. Some temperate plants, such as Arabidopsis, have the ability to carry out internal adjustment, which maintains and checks the metabolic machinery during cold temperatures. This cold acclimation process requires prior exposure to low, chilling temperatures to prevent damage dur ...
DOI:
10.3390/agronomy12030702

23. Platelet-released extracellular vesicles: the effects of thrombin activation

Author:
Rosa Suades; Teresa Padró; Gemma Vilahur; Lina Badimon
Source:
Cellular and molecular life sciences 2022 v.79 no.3 pp. 190
ISSN:
1420-682X
Subject:
adhesion; angiogenesis; cell adhesion; coagulation; cytoskeleton; flow cytometry; glycoproteins; inflammation; mass spectrometry; metabolism; phenotype; platelet activation; proteomics; signal transduction; thrombin; thrombosis
Abstract:
... Platelets exert fundamental roles in thrombosis, inflammation, and angiogenesis, contributing to different pathologies from cardiovascular diseases to cancer. We previously reported that platelets release extracellular vesicles (pEVs) which contribute to thrombus formation. However, pEV composition remains poorly defined. Indeed, pEV quality and type, rather than quantity, may be relevant in intra ...
DOI:
10.1007/s00018-022-04222-4

24. Pollen priming for more efficient reproduction in a heating world: what we know, what we need to know

Author:
Lavinia Mareri; Giampiero Cai
Source:
Plant Stress 2022 v.3 pp. 100060
ISSN:
2667-064X
Subject:
cell walls; cytoskeleton; gametophytes; heat; heat stress; heat tolerance; males; plant stress; pollen; pollen tubes; reactive oxygen species; sexual reproduction
Abstract:
... Global warming is predicted to alter temperatures thereby leading to a significant loss in crop productivity and yield. One target of global warming is plant sexual reproduction, which will be deeply threatened by high temperatures because the male gametophyte (the pollen) is particularly susceptible to heat stress. As a reaction to the latter, pollen enacts many responses that involve specific re ...
DOI:
10.1016/j.stress.2022.100060

25. Potential protective effect of lactic acid bacteria against zearalenone causing reprotoxicity in male mice

Author:
Hela Belgacem; Massimo Venditti; Jalila Ben Salah-Abbès; Sergio Minucci; Samir Abbès
Source:
Toxicon 2022 v.209 pp. 56-65
ISSN:
0041-0101
Subject:
Lactobacillus plantarum; adults; apoptosis; bioavailability; chronic toxicity; cytoskeleton; dietary supplements; digestive tract; lactic acid; males; mice; morphogenesis; oxidative stress; probiotics; protective effect; protein content; spermatogenesis; spermatozoa; zearalenone
Abstract:
... Zearalenone (ZEN) is a worldwide fusarotoxin that poses a threat to the consumer due to its chronic toxicity. Herein we examined the effects of ZEN on adult mouse testis, focusing on oxidative stress, biochemical and morphological parameters. In addition, since cytoskeletal remodeling is a key event for the production of good quality gametes, the expression and localization of two proteins, Dishev ...
DOI:
10.1016/j.toxicon.2022.02.011

26. Predicted coronavirus Nsp5 protease cleavage sites in the human proteome

Author:
Benjamin M. Scott; Vincent Lacasse; Ditte G. Blom; Peter D. Tonner; Nikolaj S. Blom
Source:
BMC genomic data 2022 v.23 no.1 pp. 25
ISSN:
2730-6844
Subject:
COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; apoptosis; bioinformatics; cysteine proteinases; cytokines; cytoskeleton; databases; genomics; humans; innate immunity; pandemic; pathogenesis; polyproteins; protein structure; proteome; viral nonstructural proteins; virus replication; viruses
Abstract:
... BACKGROUND: The coronavirus nonstructural protein 5 (Nsp5) is a cysteine protease required for processing the viral polyprotein and is therefore crucial for viral replication. Nsp5 from several coronaviruses have also been found to cleave host proteins, disrupting molecular pathways involved in innate immunity. Nsp5 from the recently emerged SARS-CoV-2 virus interacts with and can cleave human pro ...
DOI:
10.1186/s12863-022-01044-y

27. Pro-inflammatory and (Epi-)genetic markers in saliva for disease risk in childhood obesity

Author:
Daniel A. Bizjak; Ole Ammerpohl; Sebastian VW. Schulz; Janine Wendt; Jürgen M. Steinacker; Marion Flechtner-Mors
Source:
Nutrition, metabolism, and cardiovascular diseases 2022 pp. -
ISSN:
0939-4753
Subject:
DNA methylation; biomarkers; cell movement; childhood obesity; cytoskeleton; epigenetics; interleukin-17; interleukin-6; metabolism; neopterin; nutrition; saliva; surveys; variance
Abstract:
... Childhood obesity is an emerging problem often leading to earlier onset of non-communicable diseases in later life. Biomarkers to identify individual risk scores are insufficient in routine clinical practice, which is related to the need for easily sampled, non-invasive survey methods in children. We aimed to investigate and strengthen possible pro-inflammatory markers and epigenetic risk factors ...
DOI:
10.1016/j.numecd.2022.03.016

28. Proteomic analysis identifies potential markers in small white and small yellow follicle development in chickens

Author:
Armughan Ahmed Wadood; Liping Pu; Qaisar Shahzad; Muhammad Waqas; Lintian Yu; Yuying Liao; Saif ur Rehman; Dongyang Chen; Zhenwen Huang; Yangqing Lu
Source:
Reproduction, fertility, and development 2022 v.34 no.6 pp. 516-525
ISSN:
1031-3613
Subject:
RNA; amino acids; biosynthesis; carbon metabolism; chickens; cytoskeleton; egg production; follicular development; gene expression regulation; gene ontology; oocytes; peptides; proteome; proteomics; quantitative polymerase chain reaction; China
Abstract:
... Extensive knowledge of follicular development is imperative for improving egg production in chickens. The functional role of follicles to produce oocytes (eggs) is well recognised; however, specific markers associated with follicle development have been poorly explored. Therefore, a tandem mass tag based proteomic technique was used to identify the status of the proteome of small white follicles ( ...
DOI:
10.1071/RD21184

29. Regulators regulated: Different layers of control for plasma membrane phosphoinositides in plants

Author:
Mareike Heilmann; Ingo Heilmann
Source:
Current opinion in plant biology 2022 v.67 pp. 102218
ISSN:
1369-5266
Subject:
biogenesis; cytoskeleton; plant tissues; plasma membrane; transcription (genetics)
Abstract:
... The membranes of plant cells serve diverse physiological roles, which are defined largely by the localized and dynamic recruitment of proteins. Signaling lipids, such as phosphoinositides, can aid protein recruitment to the plasma membrane via specific recognition of their head groups and influence vesicular trafficking, cytoskeletal dynamics and other processes, with ramifications for plant tissu ...
DOI:
10.1016/j.pbi.2022.102218

30. Relationship of matrix stiffness and cell morphology in regulation of osteogenesis and adipogenesis of BMSCs

Author:
Yutong Guo; Yini Qiao; Shuqi Quan; Cai Yang; Juan Li
Source:
Molecular biology reports 2022 v.49 no.4 pp. 2677-2685
ISSN:
0301-4851
Subject:
actin; adipogenesis; bone formation; bone marrow; cytoskeleton; fibronectins; fluorescent antibody technique; molecular biology; tissue culture
Abstract:
... BACKGROUNDS: Matrix stiffness has been found to regulate cell morphology, while both cell morphology and matrix stiffness are verified as important factors directing BMSCs (bone marrow mesenchymal stem cells) differentiation. This study aimed to investigate whether matrix stiffness depended on cell morphology to regulate osteogenesis and adipogenesis of BMSCs on 2D substrates. METHODS AND RESULTS: ...
DOI:
10.1007/s11033-021-07075-5

31. Rho GTPases: Non‐canonical regulation by cysteine oxidation

Author:
Mackenzie Hurst; David J. McGarry; Michael F. Olson
Source:
BioEssays 2022 v.44 no.2 pp. e2100152
ISSN:
0265-9247
Subject:
adhesion; cysteine; cytoskeleton; guanosinetriphosphatase; hydrogen peroxide; methionine; oxidation; phosphorylation; thiols; transportation
Abstract:
... Rho GTPases are critically important and are centrally positioned regulators of the actomyosin cytoskeleton. By influencing the organization and architecture of the cytoskeleton, Rho proteins play prominent roles in many cellular processes including adhesion, migration, intra‐cellular transportation, and proliferation. The most important method of Rho GTPase regulation is via the GTPase cycle; how ...
DOI:
10.1002/bies.202100152

32. Structure, regulation, and host interaction of outer membrane protein U (OmpU) of Vibrio species

Author:
Hilal A. Ganie; Aaina Choudhary; Somesh Baranwal
Source:
Microbial pathogenesis 2022 v.162 pp. 105267
ISSN:
0882-4010
Subject:
Vibrio; actin; adhesion; antimicrobial peptides; aquaculture; caspases; cations; cytokines; cytoskeleton; death; epithelium; humans; immune response; outer membrane proteins; pathogenesis; peptidoglycans; reactive oxygen species; secretion; virulence
Abstract:
... OmpU is a multimeric, cation selective outer membrane protein of Vibrio and related species that non-covalently interact with peptidoglycan layer. Interaction of OmpU with human host cells triggers signaling pathways to promote cytokine secretion, reactive oxygen species production, and caspase independent death in immune and epithelial cells. Non-choleric OmpU imparts resistance to antimicrobial ...
DOI:
10.1016/j.micpath.2021.105267

33. The role of Ca2+ in the injury of host cells during the schizogenic stage of E. tenella

Author:
Xiao-Ling Lv; Yong-Yan Wang; Ming-Xue Zheng; Rui Bai; Li Zhang; Bu-Ting Duan; Xuan Lei; Xue-Song Zhang; Yong-Juan Zhao; Kai-Ling Cui; Tong Xu
Source:
Poultry science 2022 v.101 no.7 pp. 101916
ISSN:
0032-5791
Subject:
apoptosis; calcium; chick embryos; cytoskeleton; epithelial cells; epithelium; flow cytometry; fluorescence; models; necrosis; transmission electron microscopy
Abstract:
... Cecal epithelial cell damage is a key factor in host injure during the development of E. tenella. The intracellular free Ca²⁺ of the host cell is closely related to the invasion, development and proliferation of intracellular parasites, and cell damage. To determine the relationship between Ca²⁺ and host cell damage in the schizogenic stage of E. tenella, we established a chick embryo cecal epithe ...
DOI:
10.1016/j.psj.2022.101916

34. Transcriptomic investigation of the effects of TDCPP on PC12 and GC2 cells with experimental validation

Author:
Xi Zhang; Qifu Zhang; Yaohui Shan; Jingsong Xiao; Jin Cheng; Feng Ye; Yan Sai
Source:
Gene 2022 v.822 pp. 146349
ISSN:
0378-1119
Subject:
apoptosis; cell adhesion; cell cycle checkpoints; cytoskeleton; flame retardants; focal adhesions; genes; protein synthesis; reproductive system; reproductive toxicology; toxicity; transcriptome; transcriptomics; vimentin
Abstract:
... TDCPP is a flame retardant which has nervous and reproductive toxicity. Although there is a close association between nervous and reproductive system, the exact toxic mechanism of TDCPP in these systems is still seldom, especially in a genome scale. In this study, we explored the transcriptomic landscape of TDCPP in PC12 and GC2 cells using RNAseq method. A total of 465 co-differential expressed g ...
DOI:
10.1016/j.gene.2022.146349

35. Use of microscale thermophoresis to measure protein-lipid interactions

Author:
Sparks Robert P.; Lawless William; Arango Andres S.; Tajkhorshid Emad; Fratti Rutilio A.
Source:
Journal of visualized experiments 2022 no.180 pp. e60607
ISSN:
1940-087X
Subject:
calorimetry; chemical species; cost effectiveness; cytoskeleton; drugs; fluorescence; fluorescent dyes; heat; ligands; signal transduction; surface plasmon resonance; titration
Abstract:
... The ability to determine the binding affinity of lipids to proteins is an essential part of understanding protein-lipid interactions in membrane trafficking, signal transduction and cytoskeletal remodeling. Classic tools for measuring such interactions include surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC). While powerful tools, these approaches have setbacks. ITC requi ...
DOI:
10.3791/60607

36. 1α,25-(OH)2 vitamin D3 prevents insulin resistance and regulates coordinated exocytosis and insulin secretion

Author:
Ana Karla Bittencourt Mendes; Paola Miranda Sulis; Fernanda Carvalho Cavalari; Diana Patricia Rey Padilla; Marcela Aragón; Joana Margarida Gaspar; Fátima Regina Mena Barreto Silva
Source:
Journal of nutritional biochemistry 2022 v.99 pp. 108864
ISSN:
0955-2863
Subject:
blood glucose; blood serum; calcium; cholecalciferol; cytoskeleton; diazoxide; exocytosis; glibenclamide; glucose; homeostasis; insulin resistance; insulin secretion; plasma membrane; signal transduction
Abstract:
... Vitamin D₃ is associated with improvements in insulin resistance and glycemia. In this study, we investigated the short-term effect of 1α,25(OH)₂ Vitamin D₃ (1,25-D₃) and cholecalciferol (vitamin D₃) on the glycemia and insulin sensitivity of control and dexamethasone-induced insulin-resistance rats. ⁴⁵Ca²⁺ influx responses to 1,25-D₃ and its role in insulin secretion were investigated in isolated ...
DOI:
10.1016/j.jnutbio.2021.108864

37. UBXN1 is a strong candidate gene in regulation of pork water-holding capacity

Author:
J. He; X. Lin; H. Yang; Y. Tian; Y. Zhao; L. Zhang; W. Wei; J. Chen
Source:
Archiv für Tierzucht 2021 v.64 pp. 109-118
ISSN:
2363-9822
Subject:
DNA; Duroc; Large White; alleles; crossbreds; cytoskeleton; desmin; dystrophin; gene expression; genotype; genotyping; luciferase; muscles; pork; pork quality; promoter regions; single nucleotide polymorphism; water holding capacity
Abstract:
...

The UBX domain containing protein 1-like gene (UBXN1) promotes the degradation of myofibrillar proteins during meat maturation, which affects meat water-holding capacity (WHC). This study aims to identify functional mutations in UBXN1 promoter region, which affects the transcription activity and therefore the WHC. Firstly, we confirmed that the UBXN1 expression level was po ...

DOI:
10.5194/aab-64-109-2021

38. A 21-bp indel within the LLGL1 gene is significantly associated with litter size in goat

Author:
Nuan Liu; Wenbo Cui; Mingyue Chen; Xuelian Zhang; Xiaoyue Song; Chuanying Pan
Source:
Animal biotechnology 2021 v.32 no.2 pp. 213-218
ISSN:
1532-2378
Subject:
DNA; alleles; bioinformatics; biotechnology; cell polarity; cytoskeleton; epithelium; genetic markers; genotype; goats; industry; litter size; marker-assisted selection
Abstract:
... The scribble cell polarity complex component (LLGL1) is part of the cytoskeletal network and is involved in maintaining cell polarity and epithelial integrity. Based on the whole-genome sequencing analysis in goat, LLGL1 gene is suggested as a putative important candidate gene affecting litter size in Shaanbei White Cashmere Goats (SBWC). Therefore, the objective of this study was to uncover the p ...
DOI:
10.1080/10495398.2019.1677682

39. A Layered View on Focal Adhesions

Author:
Karin Legerstee; Adriaan B. Houtsmuller
Source:
Biology 2021 v.10 no.11 pp. -
ISSN:
2079-7737
Subject:
actin; cell movement; cytoskeleton; extracellular matrix; fluorescence; integrins; physiological transport; plasma membrane; talin
Abstract:
... The cytoskeleton provides structure to cells and supports intracellular transport. Actin fibres are crucial to both functions. Focal Adhesions (FAs) are large macromolecular multiprotein assemblies at the ends of specialised actin fibres linking these to the extracellular matrix. FAs translate forces on actin fibres into forces contributing to cell migration. This review will discuss recent insigh ...
DOI:
10.3390/biology10111189

40. A Mucoralean White Collar-1 Photoreceptor Controls Virulence by Regulating an Intricate Gene Network during Host Interactions

Author:
Carlos Pérez-Arques; María Isabel Navarro-Mendoza; Laura Murcia; Carlos Lax; Marta Sanchis; Javier Capilla; Eusebio Navarro; Victoriano Garre; Francisco Esteban Nicolás
Source:
Microorganisms 2021 v.9 no.2 pp. -
ISSN:
2076-2607
Subject:
Mucor; cell movement; cytoskeleton; death; dissection; fungi; genes; germination; host-pathogen relationships; phagocytosis; photoreceptors; transcriptomics; virulence; zygomycosis
Abstract:
... Mucolares are an ancient group of fungi encompassing the causal agents for the lethal infection mucormycosis. The high lethality rates, the emerging character of this disease, and the broad antifungal resistance of its causal agents are mucormycosis features that are alarming clinicians and researchers. Thus, the research field around mucormycosis is currently focused on finding specific weaknesse ...
DOI:
10.3390/microorganisms9020459

41. A Transcriptome Insight During Early Fish Larval Development Followed by Starvation in Seriola rivoliana

Author:
Danitzia A. Guerrero-Tortolero; Grecia Vázquez-Islas; Rafael Campos-Ramos
Source:
Marine biotechnology 2021 v.23 no.5 pp. 749-765
ISSN:
1436-2228
Subject:
AMP-activated protein kinase; Seriola rivoliana; absorption; autophagy; biosynthesis; biotechnology; carbohydrates; catabolism; cell cycle; circadian rhythm; cytoskeleton; energy; estrogens; gene expression regulation; immune system; larval development; marine fish; mouth; muscle development; muscles; oils; protein synthesis; sequence analysis; starvation; transcription (genetics); transcriptome; vision; yolk sac; zooplankton
Abstract:
... We investigated a time-course larval transcriptional analysis (RNA-seq) in the longfin yellowtail Seriola rivoliana, from hatching to day four at 22 °C, without providing zooplankton as food. Larval starvation is a critical physiological stage that must be prevented to ensure survival. However, the transcriptional mechanisms to endure starvation have not been investigated in marine fish. Different ...
DOI:
10.1007/s10126-021-10061-4

42. A first glimpse into the transcriptomic changes induced by the PaV1 infection in the gut of Caribbean spiny lobsters, Panulirus argus (Latreille, 1804) (Decapoda: Achelata: Palinuridae)

Author:
Ioreni Margarita Hernández-Velázquez; Jesús Alejandro Zamora-Briseño; Alejandro Pereira-Santana; Juan Pablo Huchin-Mian; Carlos Eduardo González-Penagos; Juan Antonio Pérez-Vega; Enrique Lozano-Álvarez; Patricia Briones-Fourzán; Rossanna Rodríguez-Canul
Source:
Virus research 2022 v.311 pp. 198713
ISSN:
0168-1702
Subject:
Panulirus argus; cytoskeleton; gastrointestinal system; gene expression regulation; hepatopancreas; juveniles; proteolysis; research; sequence analysis; transcription (genetics); transcriptomics; unigenes; virulent strains; viruses; Caribbean
Abstract:
... The Caribbean spiny lobster, Panulirus argus (Latreille, 1804) supports important fisheries in the Caribbean region. This species is affected by a deadly virus, Panulirus argus Virus 1 (PaV1), the only known pathogenic virus for this species. As infection progresses, the effects of PaV1 on its host become systemic, with far reaching impacts on the host's physiology, including structural injuries t ...
DOI:
10.1016/j.virusres.2022.198713

43. A proteomic view of cellular responses of macrophages to copper when added as ion or as copper-polyacrylate complex

Author:
Bastien Dalzon; Julie Devcic; Joanna Bons; Anaelle Torres; Hélène Diemer; Stéphane Ravanel; Véronique Collin-Faure; Sarah Cianférani; Christine Carapito; Thierry Rabilloud
Source:
Journal of proteomics 2021 v.239 pp. 104178
ISSN:
1874-3919
Subject:
copper; cytoskeleton; glutathione; macrophages; membrane potential; mice; mitochondria; phagocytosis; polyacrylic acid; proteomics; toxicity
Abstract:
... Copper is an essential metal for life, but is toxic at high concentrations. In mammalian cells, two copper transporters are known, CTR1 and CTR2. In order to gain insights on the possible influence of the import pathway on cellular responses to copper, two copper challenges were compared: one with copper ion, which is likely to use preferentially CTR1, and one with a copper-polyacrylate complex, w ...
DOI:
10.1016/j.jprot.2021.104178

44. A quantitative proteomic analysis reveals the potential roles of PRDX3 in neurite outgrowth in N2a-APPswe cells

Author:
Benhong Xu; Chuanyue Gao; Huan Zhang; Xinfeng Huang; Xifei Yang; Chen Yang; Wei Liu; Desheng Wu; Jianjun Liu
Source:
Biochemical and biophysical research communications 2022 v.604 pp. 144-150
ISSN:
0006-291X
Subject:
Alzheimer disease; amyloid; catabolism; cell lines; cytoskeleton; gene ontology; glutathione transferase; lipids; mitochondria; neurites; oxidoreductases; plasma membrane; protein subunits; proteomics; research; retinoic acid; serine; signal transduction
Abstract:
... Alzheimer's disease (AD) is characterized by amyloid plaques and neurofibrillary tangles accompanied by progressive neurite loss. Mitochondria play pivotal roles in AD development. PRDX3 is a mitochondrial peroxide reductase critical for H₂O₂ scavenging and signal transduction. In this study, we found that PRDX3 knockdown (KD) in the N2a-APPswe cell line promoted retinoic acid (RA)-induced neurite ...
DOI:
10.1016/j.bbrc.2022.03.021

45. A role for the last C-terminal helix of the F plasmid segregating protein SopA in nucleoid binding and plasmid maintenance

Author:
Dipika Mishra; Anirudh Jakhmola; Ramanujam Srinivasan
Source:
Plasmid 2022 v.119-120 pp. 102617
ISSN:
0147-619X
Subject:
adenosinetriphosphatase; antibiotic resistance; cytoskeleton; plasmids
Abstract:
... The rapid emergence and spread of antibiotic resistance is a growing global burden. Antibiotic resistance is often associated with large single or low copy number plasmids, which rely upon cytoskeletal proteins for their stable maintenance. While the mechanism of plasmid partitioning has been well established for the R plasmids, the molecular details by which the F plasmid is maintained is only be ...
DOI:
10.1016/j.plasmid.2022.102617

46. Active biomaterials for mechanobiology

Author:
Berna Özkale; Mahmut Selman Sakar; David J. Mooney
Source:
Biomaterials 2021 v.267 pp. 120497
ISSN:
0142-9612
Subject:
biocompatible materials; biophysics; biopolymers; cell movement; cytoskeleton; fibrosis; heat; mammals; mechanotransduction; metastasis; therapeutics; tissue repair; transcription (genetics)
Abstract:
... Active biomaterials offer novel approaches to study mechanotransduction in mammalian cells. These material systems probe cellular responses by dynamically modulating their resistance to endogenous forces or applying exogenous forces on cells in a temporally controlled manner. Stimuli-responsive molecules, polymers, and nanoparticles embedded inside cytocompatible biopolymer networks transduce exte ...
DOI:
10.1016/j.biomaterials.2020.120497

47. Alkaloid-rich fraction of Ervatamia coronaria sensitizes colorectal cancer through modulating AMPK and mTOR signalling pathways

Author:
Naibedya Dutta; Deepak Bharadwaj Pemmaraju; Suvranil Ghosh; Asif Ali; Ayan Mondal; Chirantan Majumder; Vinod K. Nelson; Subhash C. Mandal; Anup K. Misra; Aravind K. Rengan; Velyutham Ravichandiran; Chun-Tao Che; Katerina V. Gurova; Andrei V. Gudkov; Mahadeb Pal
Source:
Journal of ethnopharmacology 2022 v.283 pp. 114666
ISSN:
0378-8741
Subject:
DNA; Tabernaemontana divaricata; apoptosis; autophagy; cell cycle checkpoints; colorectal neoplasms; cyclin-dependent kinase; cytoskeleton; death; drug therapy; fractionation; immunohistochemistry; interphase; leaves; mechanism of action; ornamental plants; phosphorylation; toxicity; traditional medicine; India
Abstract:
... Ervatamia coronaria, a popular garden plant in India and some other parts of the world is known traditionally for its anti-inflammatory and anti-cancer properties. The molecular bases of these functions remain poorly understood.Efficacies of the existing therapies for colorectal cancer (CRC) are limited by their life-threatening side effects and unaffordability. Therefore, identifying a safer, eff ...
DOI:
10.1016/j.jep.2021.114666

48. Alterations in Taenia crassiceps cysticerci cytoskeleton induced by nitazoxanide and flubendazole

Author:
Nayana Ferreira de Lima; Guaraciara de Andrade Picanço; Diana Gabriela Ríos Valencia; Edgar Oliver López Villegas; María Del Rosário Espinoza Mellado; Javier R. Ambrosio; Marina Clare Vinaud
Source:
Acta tropica 2021 v.221 pp. 106027
ISSN:
0001-706X
Subject:
Taenia crassiceps; Taenia solium; Western blotting; actin; albendazole; central nervous system; cysticerci; cysticercosis; cytoskeleton; electrophoresis; eyes; flubendazole; larvae; models; parasites; praziquantel; transmission electron microscopy; tubulin
Abstract:
... Cysticercosis is the presence of Taenia solium larval stage in tissues such as central nervous system, skin, muscles and eye globe. The current treatment is based on albendazole and praziquantel which already present resistance reports. Therefore, the search for alternative treatments is paramount. The aim of this study was to determine the effect of flubendazole and nitazoxanide on cytoskeleton p ...
DOI:
10.1016/j.actatropica.2021.106027

49. Alzheimer-like cell death after vanadium pentoxide inhalation

Author:
Enrique Montiel-Flores; Oscar A. Mejía-García; Jose Luis Ordoñez-Librado; Ana Luisa Gutierrez-Valdez; Jesús Espinosa-Villanueva; Claudia Dorado-Martínez; Leonardo Reynoso-Erazo; Rocio Tron-Alvarez; Vianey Rodríguez-Lara; Maria Rosa Avila-Costa
Source:
Heliyon 2021 v.7 no.8 pp. e07856
ISSN:
2405-8440
Subject:
Alzheimer disease; cell death; cerebellum; cytoskeleton; death; hippocampus; lipid peroxidation; males; necrosis; oral administration; oxidation; toxicity; vanadium
Abstract:
... Vanadium (V) toxicity depends on its oxidation state; it seems that vanadium pentoxide (V₂O₅) is the most toxic to the living cells. It has been reported that oral administration induces changes in motor activity and learning; in rats, I.P. administration increases lipid peroxidation levels in the cerebellum and the concentration of free radicals in the hippocampus and cerebellum. Mice that inhale ...
DOI:
10.1016/j.heliyon.2021.e07856

50. Analysis of major sperm proteins in two nematode species from two classes, Enoplus brevis (Enoplea, Enoplida) and Panagrellus redivivus (Chromadorea, Rhabditida), reveals similar localization, but less homology of protein sequences than expected for Nematoda phylum

Author:
Julia K. Zograf; Yulia A. Trebukhova; Vladimir V. Yushin; Konstantin V. Yakovlev
Source:
Organisms, diversity, & evolution 2022 v.22 no.1 pp. 117-130
ISSN:
1439-6092
Subject:
Enoplida; Enoplus; Panagrellus redivivus; cytoskeleton; evolution; sperm capacitation; spermatozoa
Abstract:
... Major sperm proteins (MSPs) are a nematode-specific system of cytoskeletal proteins required for amoeboid sperm movement. A number of MSP genes vary in different nematode species, but encoded protein sequences reveal high homology between these proteins. However, all studies of MSP localization and functions to date are based exclusively on the representatives of the order Rhabditida belonging to ...
DOI:
10.1007/s13127-021-00522-y

51. Antitumor Effects of Scorpion Peptide Smp43 through Mitochondrial Dysfunction and Membrane Disruption on Hepatocellular Carcinoma

Author:
Jinwei Chai; Wanren Yang; Yahua Gao; Ruiyin Guo; Qing Peng; Mohamed A. Abdel-Rahman; Xueqing Xu
Source:
Journal of natural products 2021 v.84 no.12 pp. 3147-3160
ISSN:
1520-6025
Subject:
Scorpiones; antimicrobial peptides; apoptosis; autophagy; cell cycle checkpoints; cell viability; cyclins; cytoskeleton; cytotoxicity; decline; endocytosis; hepatoma; humans; membrane potential; mitochondria; mitochondrial membrane; necrosis; venoms; xenotransplantation
Abstract:
... Smp43, a cationic antimicrobial peptide identified from the venom gland of the Egyptian scorpion Scorpio maurus palmatus, shows cytotoxicity toward hepatoma cell line HepG2 by membrane disruption. However, its underlying detailed mechanisms still remain to be further clarified. In the present study, we evaluated the cellular internalization of Smp43 and explored its effects on cell viability, cell ...
DOI:
10.1021/acs.jnatprod.1c00963

52. Arachidonic Acid as Mechanotransducer of Renin Cell Baroreceptor

Author:
Undurti N. Das
Source:
Nutrients 2022 v.14 no.4 pp. -
ISSN:
2072-6643
Subject:
DNA; arachidonic acid; atherosclerosis; blood pressure; blood volume; cell membrane structures; chromatin; coronary disease; cytoskeleton; diabetes mellitus; gene expression; homeostasis; hypertension; insulin; mechanotransduction; metabolites; myosin; neurotransmitters; peroxidation; phospholipases; renin; rheumatoid arthritis; secretion; transient receptor potential vanilloid channels
Abstract:
... For normal maintenance of blood pressure and blood volume a well-balanced renin-angiotensin-aldosterone system (RAS) is necessary. For this purpose, renin is secreted as the situation demands by the juxtaglomerular cells (also called as granular cells) that are in the walls of the afferent arterioles. Juxtaglomerular cells can sense minute changes in the blood pressure and blood volume and accordi ...
DOI:
10.3390/nu14040749

53. Atractylodis rhizoma water extract attenuates fructose-induced glomerular injury in rats through anti-oxidation to inhibit TRPC6/p-CaMK4 signaling

Author:
Li Chen; Jie Yang; Si-Jie Zhao; Tu-Shuai Li; Rui-Qing Jiao; Ling-Dong Kong
Source:
Phytomedicine 2021 v.91 pp. 153643
ISSN:
0944-7113
Subject:
Oriental traditional medicine; antioxidant activity; calcium-calmodulin-dependent protein kinase; catalase; cortex; cytoskeleton; diaphragm; drugs; edema; fructose; hydrogen peroxide; kidneys; males; malondialdehyde; oxidative stress; pharmacology; rats; rhizomes; superoxide dismutase; tandem mass spectrometry; transient receptor potential channels; urine
Abstract:
... Atractylodis rhizoma, an aromatic herb for resolving dampness, is used to treat Kidney-related edema in traditional Chinese medicine for thousands years. This herb possesses antioxidant effect. However, it is not yet clear how Atractylodis rhizoma prevents glomerular injury through its anti-oxidation.Based the analysis of Atractylodis rhizoma water extract (ARE) components and network pharmacology ...
DOI:
10.1016/j.phymed.2021.153643

54. Autism-associated ANK2 regulates embryonic neurodevelopment

Author:
Shotaro Kawano; Masayuki Baba; Hotaka Fukushima; Daiki Miura; Hitoshi Hashimoto; Takanobu Nakazawa
Source:
Biochemical and biophysical research communications 2022 v.605 pp. 45-50
ISSN:
0006-291X
Subject:
autism; cell differentiation; cerebral cortex; cytoskeleton; electroporation; genes; haploinsufficiency; membrane proteins; mice; neural stem cells; neurodevelopment; neurons; pathogenesis; research; risk
Abstract:
... Autism spectrum disorder (ASD) is a neurodevelopmental condition characterized by altered social communication, restricted interests, and stereotypic behaviors. Although the molecular and cellular pathogeneses of ASD remain elusive, impaired neural stem cell differentiation and neuronal migration during cortical development are suggested to be critically involved in ASD. ANK2, which encodes for a ...
DOI:
10.1016/j.bbrc.2022.03.058

55. Avoiding tensional equilibrium in cells migrating on a matrix with cell-scale stiffness-heterogeneity

Author:
Hiroyuki Ebata; Satoru Kidoaki
Source:
Biomaterials 2021 v.274 pp. 120860
ISSN:
0142-9612
Subject:
biocompatible materials; cytoskeleton; extracellular matrix; finite element analysis; microscopy
Abstract:
... Intracellular stresses affect various cell functions, including proliferation, differentiation and movement, which are dynamically modulated in migrating cells through continuous cell-shaping and remodeling of the cytoskeletal architecture induced by spatiotemporal interactions with extracellular matrix stiffness. When cells migrate on a matrix with cell-scale stiffness-heterogeneity, which is a c ...
DOI:
10.1016/j.biomaterials.2021.120860

56. Bacterial Vipp1 and PspA are members of the ancient ESCRT-III membrane-remodeling superfamily

Author:
Jiwei Liu; Matteo Tassinari; Diorge P. Souza; Souvik Naskar; Jeffrey K. Noel; Olga Bohuszewicz; Martin Buck; Tom A. Williams; Buzz Baum; Harry H. Low
Source:
Cell 2021 v.184 no.14 pp. 3660-3673.e18
ISSN:
0092-8674
Subject:
Nostoc punctiforme; ancestry; cryo-electron microscopy; eukaryotic cells; photosynthesis; plastids
Abstract:
... Membrane remodeling and repair are essential for all cells. Proteins that perform these functions include Vipp1/IM30 in photosynthetic plastids, PspA in bacteria, and ESCRT-III in eukaryotes. Here, using a combination of evolutionary and structural analyses, we show that these protein families are homologous and share a common ancient evolutionary origin that likely predates the last universal com ...
DOI:
10.1016/j.cell.2021.05.041

57. Biocompatibility and Antibiofilm Properties of Calcium Silicate-Based Cements: An In Vitro Evaluation and Report of Two Clinical Cases

Author:
Maurizio Bossù; Patrizia Mancini; Erika Bruni; Daniela Uccelletti; Adele Preziosi; Marco Rulli; Michela Relucenti; Orlando Donfrancesco; Flavia Iaculli; Gianni Di Giorgio; Roberto Matassa; Alessandro Salucci; Antonella Polimeni
Source:
Biology 2021 v.10 no.6 pp. -
ISSN:
2079-7737
Subject:
Streptococcus mutans; biocompatibility; biofilm; bone formation; calcium; cell viability; cement; cytoskeleton; cytotoxicity; endodontics; fluorescent antibody technique; humans; reactive oxygen species; sarcoma; viability assays
Abstract:
... Calcium silicate-based cements have reached excellent levels of performance in endodontics, providing predictable and successful results. To better assess the properties of these bioactive materials, the present study aimed to compare the biocompatibility and antibiofilm properties of ProRoot MTA and Biodentine. Human osteogenic sarcoma (Saos-2) cells were cultured on ProRoot MTA and Biodentine sa ...
DOI:
10.3390/biology10060470

58. CASK regulates Notch pathway and functions as a tumor promoter in pancreatic cancer

Author:
Jun Qu; Yeqi Zhou; Yuan Li; Jinsong Yu; Wei Wang
Source:
Archives of biochemistry and biophysics 2021 v.701 pp. 108789
ISSN:
0003-9861
Subject:
Western blotting; apoptosis; biophysics; cell proliferation; computer software; cytoskeleton; databases; flow cytometry; metastasis; non-specific serine/threonine protein kinase; pancreatic neoplasms
Abstract:
... Calcium/calmodulin-dependent serine protein kinase (CASK), a member of membrane-associated guanylate kinase (MAGUK) super-family, is implicated in regulating cell proliferation, cytoskeletal remodeling, and cell metastasis. Our study aimed to investigate the effect of CASK on the malignant behaviors of pancreatic cancer cells and to determine the signaling pathway involved. CASK expression in panc ...
DOI:
10.1016/j.abb.2021.108789

59. Candidatus Liberibacter asiaticus manipulates the expression of vitellogenin, cytoskeleton, and endocytotic pathway-related genes to become circulative in its vector, Diaphorina citri (Hemiptera: Psyllidae)

Author:
Damini Jaiswal; V. Kavi Sidharthan; Susheel Kumar Sharma; Richa Rai; Nandlal Choudhary; Amalendu Ghosh; Virendra Kumar Baranwal
Source:
3 Biotech 2021 v.11 no.2 pp. 88
ISSN:
2190-572X
Subject:
Candidatus Liberibacter asiaticus; Citrus; Diaphorina citri; Psyllidae; cytoskeleton; energy; extensin; gene expression regulation; greening disease; insect vectors; laminin; transcriptomics; tropomyosins; troponin C; vitellogenin
Abstract:
... Citrus greening disease or huanglongbing (HLB) caused by Candidatus Liberibacter asiaticus (CLas) limits citrus production worldwide. CLas is transmitted by the Asian citrus psyllid (ACP), Diaphorina citri (Hemiptera: Psyllidae) in a persistent-propagative manner. Understanding the molecular interaction between CLas and ACP and interrupting the interrelationship can provide an alternative to insec ...
DOI:
10.1007/s13205-021-02641-x

60. Cell Cytoskeleton and Stiffness Are Mechanical Indicators of Organotropism in Breast Cancer

Author:
Kai Tang; Ying Xin; Keming Li; Xi Chen; Youhua Tan
Source:
Biology 2021 v.10 no.4 pp. -
ISSN:
2079-7737
Subject:
actin; brain; breast neoplasms; cytoskeleton; gene expression regulation; mechanics; metastasis; neoplasm cells
Abstract:
... Tumor metastasis involves the dissemination of tumor cells from the primary lesion to other organs and the subsequent formation of secondary tumors, which leads to the majority of cancer-related deaths. Clinical findings show that cancer cell dissemination is not random but exhibits organ preference or organotropism. While intrinsic biochemical factors of cancer cells have been extensively studied ...
DOI:
10.3390/biology10040259

61. Cell polarity control by Wnt morphogens

Author:
Chung-Kuan Chen; Chun-Liang Pan
Source:
Developmental biology 2022 v.487 pp. 34-41
ISSN:
0012-1606
Subject:
Caenorhabditis elegans; asymmetry; axons; cell polarity; cytoskeleton; glycoproteins; mitosis; transcription (genetics)
Abstract:
... Cell polarity is regulated by both intrinsic properties of the cell and extrinsic factors in the environment. Wnts are secreted glycoproteins in graded distribution, and they function as morphogens to instruct cell fate and as guidance cues to steer axon growth cone, respectively. Recent studies suggest that Wnts also instruct cell polarization in diverse contexts, by engaging cytoskeletal machine ...
DOI:
10.1016/j.ydbio.2022.04.007

62. Cellular bases of olfactory circuit assembly revealed by systematic time-lapse imaging

Author:
Tongchao Li; Tian-Ming Fu; Kenneth Kin Lam Wong; Hongjie Li; Qijing Xie; David J. Luginbuhl; Mark J. Wagner; Eric Betzig; Liqun Luo
Source:
Cell 2021 v.184 no.20 pp. 5107-5121.e14
ISSN:
0092-8674
Subject:
Drosophila; innervation; microscopy; nerve tissue; olfactory receptors
Abstract:
... Neural circuit assembly features simultaneous targeting of numerous neuronal processes from constituent neuron types, yet the dynamics is poorly understood. Here, we use the Drosophila olfactory circuit to investigate dynamic cellular processes by which olfactory receptor neurons (ORNs) target axons precisely to specific glomeruli in the ipsi- and contralateral antennal lobes. Time-lapse imaging o ...
DOI:
10.1016/j.cell.2021.08.030

63. Characterization and Functional Study of FAM49B Reveals Its Effect on Cell Proliferation in HEK293T Cells

Author:
Yijian Chen; Yuyan Jiang; Jihui Lao; Yankuan Zhou; Lida Su; Xiao Huang
Source:
Genes 2022 v.13 no.2 pp. -
ISSN:
2073-4425
Subject:
CRISPR-Cas systems; cell lines; cell proliferation; cyclins; cytoskeleton; gene expression; sequence homology
Abstract:
... FAM49B/Fam49b is a member of the Fam49 (Family with sequence similarity 49) gene family, which is characterized by the conserved domain, DUF1394 (Domain of Unknown Function 1394). It has also been named CYRI-B (CYFIP related RAC1 interactor B), implicating its important function of regulating RAC1-driven cytoskeleton remolding. In this study, to further investigate its functions and mechanisms aff ...
DOI:
10.3390/genes13020388

64. Citrinin impairs pig oocyte maturation by inducing oxidative stress and apoptosis

Author:
Wen-Jie Jiang; Wen Liu; Ying-Hua Li; Hao Jiang; Yong-Nan Xu; Nam-Hyung Kim
Source:
Toxicon 2022 v.205 pp. 84-90
ISSN:
0041-0101
Subject:
Aspergillus; Monascus; Penicillium; actin; apoptosis; caspase-3; cathepsin B; citrinin; cytoskeleton; dose response; extrusion; female reproductive system; meiosis; oocytes; oxidative stress; polyketides; reactive oxygen species; swine; toxicity
Abstract:
... Citrinin (CTN) is a polyketide mycotoxin produced by several strains of Penicillium, Monascus, and Aspergillus. While CTN poses various toxic effects on the female reproductive system in animals, its direct effects on germ cell development are unclear. This study aimed to evaluate the effects of increasing concentrations of CTN (0,20,40,80,100 μM) on porcine oocyte in vitro maturation. Our results ...
DOI:
10.1016/j.toxicon.2021.11.124

65. Combined Quantitative (Phospho)proteomics and Mass Spectrometry Imaging Reveal Temporal and Spatial Protein Changes in Human Intestinal Ischemia–Reperfusion

Author:
Anna M. Kip; Juan Manuel Valverde; Maarten Altelaar; Ron M. A. Heeren; Inca H. R. Hundscheid; Cornelis H. C. Dejong; Steven W. M. Olde Damink; Benjamin Balluff; Kaatje Lenaerts
Source:
Journal of proteome research 2021 v.21 no.1 pp. 49-66
ISSN:
1535-3907
Subject:
RNA; complement; cyclin-dependent kinase; cytoskeleton; desorption; extracellular matrix; humans; innate immunity; intercellular junctions; intestinal absorption; intestines; ischemia; liquid chromatography; microvilli; mitogen-activated protein kinase; morbidity; mortality; pathophysiology; peptides; phosphoproteome; phosphorylation; proteomics; research; spatial data; tandem mass spectrometry; therapeutics
Abstract:
... Intestinal ischemia–reperfusion (IR) injury is a severe clinical condition, and unraveling its pathophysiology is crucial to improve therapeutic strategies and reduce the high morbidity and mortality rates. Here, we studied the dynamic proteome and phosphoproteome in the human intestine during ischemia and reperfusion, using liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis to gai ...
DOI:
10.1021/acs.jproteome.1c00447

66. Comparative Proteomic Analysis of Young and Adult Bull (Bos taurus) Cryopreserved Semen

Author:
Błażej Westfalewicz; Mariola Słowińska; Sylwia Judycka; Andrzej Ciereszko; Mariola A. Dietrich
Source:
Animals 2021 v.11 no.7 pp. -
ISSN:
2076-2615
Subject:
adults; bulls; cryopreservation; cytoskeleton; energy; flow cytometry; male reproductive system; oxidative stress; proteome; proteomics; semen quality; spermatozoa
Abstract:
... The age of the bull is widely accepted to influence the production of sperm, affecting the amount and quality of produced semen, which in turn impacts the results of cryopreservation. However, the exact influence of the maturation process on cryopreserved sperm, as well as the underlying molecular mechanisms of this process, are not fully understood. The goal of this study was to evaluate changes ...
DOI:
10.3390/ani11072013

67. Comparative Transcriptome Identifies Gene Expression Networks Regulating Developmental Pollen Abortion in Ogura Cytoplasmic Male Sterility in Chinese Cabbage (Brassica rapa ssp. pekinensis)

Author:
Lijiao Hu; Xiaowei Zhang; Yuxiang Yuan; Zhiyong Wang; Shuangjuan Yang; Ruina Li; Ujjal Kumar Nath; Yanyan Zhao; Baoming Tian; Gongyao Shi; Zhengqing Xie; Fang Wei; Xiaochun Wei
Source:
Horticulturae 2021 v.7 no.6 pp. -
ISSN:
2311-7524
Subject:
Brassica rapa subsp. pekinensis; Chinese cabbage; NADP-glucose-6-phosphate dehydrogenase; Raphanus sativus; abortion (plants); cluster analysis; cytoplasmic male sterility; cytoskeleton; exine; gene expression; gene expression regulation; glutathione; lignin; meiosis; microsporogenesis; mitosis; pollen; radishes; sporopollenin; transcriptome
Abstract:
... Ogura cytoplasmic male sterility (Ogura CMS), originally identified in wild radish (Raphanus sativus), has enabled complete pollen sterility in Brassica plants, but the underlying mechanism in Ogura CMS Chinese cabbage (Brassica rapa ssp. pekinensis) remains unclear. In this study cytological analysis showed that during microsporogenesis the meiosis occurred normally, and the uninucleated pollens ...
DOI:
10.3390/horticulturae7060157

68. Comparative iTRAQ proteomics identified proteins associated with sperm maturation between yak and cattleyak epididymis

Author:
Wangsheng Zhao; Siraj Ahmed; Junxia Liu; Saeed Ahmed; Eugene Quansah; Tajmal Hussain Solangi; Yitao Wu; Yueling Yangliu; Hongmei Wang; Jiangjiang Zhu; Xin Cai
Source:
BMC veterinary research 2021 v.17 no.1 pp. 255
ISSN:
1746-6148
Subject:
acetylation; bioinformatics; boars; cell adhesion; cell communication; cytoskeleton; epididymis; gene ontology; humans; proteomics; rats; spermatozoa; stress response; two-dimensional gel electrophoresis; veterinary medicine; yaks
Abstract:
... BACKGROUND: During maturation, spermatozoa acquire motility and fertilizing capacity as they transit through the epididymis. In recent years, two-dimensional gel electrophoresis has been employed in proteomics studies conducted in rat, boar and human. However, there has not been a complete information regarding the proteins associated with sperm maturation in the epididymis. In this study, we empl ...
DOI:
10.1186/s12917-021-02907-9

69. Comparative organization of tubulin microtubules in root cells of Zea mays (Poaceae) and Beta vulgaris (Chenopodiaceae s. str. / Amaranthaceae s. l.) under the influence of clinorotation

Author:
G.V. Shevchenko
Source:
Ukrai̤nskyĭ botanychnyĭ zhurnal 2021 v.78 no.6 pp. 426-433
ISSN:
2415-8860
Subject:
Beta vulgaris; Zea mays; cell differentiation; meristems; microtubules; paclitaxel; rhizosphere; root growth; tubulin
Abstract:
... In order to identify the mechanism of functioning of the tubulin cytoskeleton, we have investigated the impact of clinorotation on cortical microtubules organization in the process of cell differentiation in growth zones of plant roots of Zea mays and Beta vulgaris. The similar organization of cortical and endoplasmic microtubules’ network in both species is noted. Clinorotation did not significan ...
DOI:
10.15407/ukrbotj78.06.426

70. Comparative proteomics for an in-depth understanding of bioadhesion mechanisms and evolution across metazoans

Author:
Chuang Liu
Source:
Journal of proteomics 2022 v.256 pp. 104506
ISSN:
1874-3919
Subject:
Animalia; bioadhesion; bioadhesives; biotechnology; cytoskeleton; dihydroxyphenylalanine; epidermal growth factor; evolution; freshwater; glycine (amino acid); invertebrates; peroxidase; proteinases; proteome; proteomics
Abstract:
... Bioadhesion is a critical process for many marine and freshwater invertebrate animals. Bioadhesives mainly made of proteins have remarkable adhesive ability underwater. Unraveling the molecular composition of bioadhesives is fundamental to understanding their physiological roles as well as their potential for biotechnology applications and antibiofouling strategies. With the development of high-th ...
DOI:
10.1016/j.jprot.2022.104506

71. Comparative transcriptome profiling of horseshoe crab Tachypleus gigas hemocytes in response to lipopolysaccharides

Author:
Maria E. Sarmiento; Kai Ling Chin; Nyok Sean Lau; Ismail Aziah; Noraznawati Ismail; Mohd Nor Norazmi; Armando Acosta; Nik Soriani Yaacob
Source:
Fish & shellfish immunology 2021 v.117 pp. 148-156
ISSN:
1050-4648
Subject:
Tachypleus; cytoskeleton; fish; gene expression regulation; hemocytes; immune response; innate immunity; lipopolysaccharides; shellfish; signal transduction; stress response; transcriptome; transcriptomics
Abstract:
... Horseshoe crabs (HSCs) are living fossil species of marine arthropods with a long evolutionary history spanning approximately 500 million years. Their survival is helped by their innate immune system that comprises cellular and humoral immune components to protect them against invading pathogens. To help understand the genetic mechanisms involved, the present study utilised the Illumina HiSeq plat ...
DOI:
10.1016/j.fsi.2021.08.001

72. Compartmentalization of mRNAs in the giant, unicellular green alga Acetabularia acetabulum

Author:
Ina J. Andresen; Russell J.S. Orr; Kamran Shalchian-Tabrizi; Jon Bråte
Source:
Algal research 2021 v.59 pp. 102440
ISSN:
2211-9264
Subject:
Acetabularia; DNA replication; acetabulum; adults; algae; cytoskeleton; gene expression; genes; morphogenesis; photosynthesis; physiological transport; research
Abstract:
... Acetabularia acetabulum is a single-celled green alga which has historically been an important model system in cell biology. Here, we attempt to re-introduce A. acetabulum as a model system through the investigation of the subcellular localization of mRNAs. A. acetabulum is a macroscopic single-celled alga with a highly complex cellular morphology. The cell is gigantic, and the single nucleus, whi ...
DOI:
10.1016/j.algal.2021.102440

73. Competitive Microtubule Binding of PEX14 Coordinates Peroxisomal Protein Import and Motility

Author:
Maren Reuter; Hamed Kooshapur; Jeff-Gordian Suda; Stefan Gaussmann; Alexander Neuhaus; Lena Brühl; Pratima Bharti; Martin Jung; Wolfgang Schliebs; Michael Sattler; Ralf Erdmann
Source:
Journal of molecular biology 2021 v.433 no.5 pp. 166765
ISSN:
0022-2836
Subject:
dissociation; humans; kinesin; ligands; microtubules; molecular biology; peroxisomes; protein transport; surfactants; tubulin
Abstract:
... Human PEX14 plays a dual role as docking protein in peroxisomal protein import and as peroxisomal anchor for microtubules (MT), which relates to peroxisome motility. For docking, the conserved N-terminal domain of PEX14 (PEX14-NTD) binds amphipathic alpha-helical ligands, typically comprising one or two aromatic residues, of which human PEX5 possesses eight. Here, we show that the PEX14-NTD also b ...
DOI:
10.1016/j.jmb.2020.166765

74. Comprehensive analysis of LIM gene family in wheat reveals the involvement of TaLIM2 in pollen development

Author:
Xuetong Yang; Yaning Bu; Fuqiang Niu; Yujie Cun; Lingli Zhang; Xiyue Song
Source:
Plant science 2022 v.314 pp. 111101
ISSN:
0168-9452
Subject:
Arabidopsis thaliana; Helianthus annuus; Setaria italica; cytoskeleton; gene expression; genes; genome-wide association study; germination; hybrids; metabolism; phenotype; phylogeny; pollen; rice; sequence analysis; tobacco; wheat
Abstract:
... LIM domain proteins were involved in organizing the cytoskeleton, adjusting the metabolism and gene expression, some of them were specific express in pollen. LIM gene family in plants were studied in sunflower, tobacco, foxtail millet, rape, rice and Arabidopsis thaliana, however, it has not been investigated in wheat to date. In the present study, we totally characterized 29 TaLIM genes through g ...
DOI:
10.1016/j.plantsci.2021.111101

75. Conquering the Nuclear Envelope Barriers by EBV Lytic Replication

Author:
Chung-Pei Lee; Mei-Ru Chen
Source:
Viruses 2021 v.13 no.4 pp. -
ISSN:
1999-4915
Subject:
DNA; Human gammaherpesvirus 4; animal pathogens; capsid; cytoskeleton; gene expression; nuclear lamina; nuclear pore; nucleocytoplasmic transport; pathogenesis
Abstract:
... The nuclear envelope (NE) of eukaryotic cells has a highly structural architecture, comprising double lipid-bilayer membranes, nuclear pore complexes, and an underlying nuclear lamina network. The NE structure is held in place through the membrane-bound LINC (linker of nucleoskeleton and cytoskeleton) complex, spanning the inner and outer nuclear membranes. The NE functions as a barrier between th ...
DOI:
10.3390/v13040702

76. Construction of adenovirus vector expressing duck sclerostin and its induction effect on myogenic proliferation and differentiation in vitro

Author:
Hehe Liu; Yanying Li; Qian Xu; Jianmei Wang; Chunchun Han; Lili Bai; Liang Li
Source:
Molecular biology reports 2022 v.49 no.4 pp. 3187-3196
ISSN:
0301-4851
Subject:
cell differentiation; cell proliferation; cytoskeleton; ducks; embryogenesis; flow cytometry; fluorescent antibody technique; mesoderm; molecular biology; morphogenesis; muscle development; muscles; myoblasts; myotubes; transcriptome; transfection
Abstract:
... BACKGROUND: Bones and muscles originated together from the mesoderm during embryogenesis, and they can influence each other through mechanical stimulations and chemical signals. The sclerostin (SOST) is secreted from mature osteocytes. Here, we used a bird model to illustrate the potential roles of SOST on duck myoblasts to verify the hypothesis that SOST might play functions in coordinating the d ...
DOI:
10.1007/s11033-022-07151-4

77. Correlated fluorescence microscopy and multi-ion beam secondary ion mass spectrometry imaging reveals phosphatidylethanolamine increases in the membrane of cancer cells over-expressing the molecular chaperone subunit CCTδ

Author:
John S. Fletcher; Sanna Sämfors; Josefine Vallin; Andreas Svanström; Julie Grantham
Source:
Analytical and bioanalytical chemistry 2021 v.413 no.2 pp. 445-453
ISSN:
1618-2642
Subject:
analytical chemistry; carbon dioxide; cell growth; cell membranes; cell proliferation; cytoskeleton; fluorescence; fluorescence microscopy; lipid composition; mass spectrometry; membrane fluidity; metastasis; molecular chaperones; multivariate analysis; neoplasm cells; neoplasms; phosphatidylethanolamines; polypeptides; transfection
Abstract:
... Changes in the membrane composition of sub-populations of cells can influence different properties with importance to tumour growth, metastasis and treatment efficacy. In this study, we use correlated fluorescence microscopy and ToF-SIMS with C₆₀⁺ and (CO₂)₆ₖ⁺ ion beams to identify and characterise sub-populations of cells based on successful transfection leading to over-expression of CCTδ, a comp ...
DOI:
10.1007/s00216-020-03013-9

78. Costain 1 (ARM19800.1) - The first identified protein of the costa of the pathogenic protozoan Tritrichomonas foetus

Author:
Paula Terra Bandeira; Wanderley de Souza
Source:
Experimental parasitology 2022 v.232 pp. 108177
ISSN:
0014-4894
Subject:
Tritrichomonas foetus; amino acid sequences; cytoskeleton; drug therapy; electron microscopy; fluorescent antibody technique; immunocytochemistry; parasitology; proteomics; protists; serodiagnosis; trichomoniasis; vaccine development
Abstract:
... Protists members of the Trichomonadidae and Tritrichomonadidae families include agents of trichomoniasis that constitute important parasitic diseases in humans and in animals of veterinary interest. One of the characteristic features of these eukaryotic microorganisms is that they contain a fibrous structure known as the costa as an important cytoskeleton structure, that differs in several aspects ...
DOI:
10.1016/j.exppara.2021.108177

79. Critical Frequency and Critical Stretching Rate for Reorientation of Cells on a Cyclically Stretched Polymer in a Microfluidic Chip

Author:
Tianjiao Mao; Yingning He; Yexin Gu; Yuqian Yang; Yue Yu; Xinlei Wang; Jiandong Ding
Source:
ACS applied materials & interfaces 2021 v.13 no.12 pp. 13934-13948
ISSN:
1944-8252
Subject:
actin; biocompatible materials; biomechanics; cell adhesion; humans; mechanotransduction; models; organ-on-a-chip; polydimethylsiloxane
Abstract:
... The ability of cells to sense and respond to mechanical signals from their surrounding microenvironments is one of the key issues in tissue engineering and regeneration, yet a fundamental study of cells with both cell observation and mechanical stimulus is challenging and should be based upon an appropriate microdevice. Herein we designed and fabricated a two-layer microfluidic chip to enable simu ...
DOI:
10.1021/acsami.0c21186

80. Cyanotoxins within and Outside of Microcystis aeruginosa Cause Adverse Effects in Rainbow Trout (Oncorhynchus mykiss)

Author:
René S. Shahmohamadloo; Xavier Ortiz Almirall; Denina B. D. Simmons; John S. Lumsden; Satyendra P. Bhavsar; Trudy Watson-Leung; Angela Vander Eyken; Gabrielle Hankins; Kate Hubbs; Polina Konopelko; Michael Sarnacki; Damon Strong; Paul K. Sibley
Source:
Environmental science & technology 2021 v.55 no.15 pp. 10422-10431
ISSN:
1520-5851
Subject:
DNA repair; Microcystis aeruginosa; Oncorhynchus mykiss; acute exposure; adults; apoptosis; cytoskeleton; freshwater fish; hemorrhage; histopathology; juveniles; metabolism; microcystins; muscular diseases; necrosis; poisonous algae; proteomics; pyruvic acid; risk; technology; toxicity
Abstract:
... The global expansion of toxic Microcystis blooms, and production of cyanotoxins including microcystins, are an increasing risk to freshwater fish. Differentiating intracellular and extracellular microcystin toxicity pathways (i.e., within and outside of cyanobacterial cells) in fish is necessary to assess the severity of risks to populations that encounter harmful algal blooms in pre-to-postsenesc ...
DOI:
10.1021/acs.est.1c01501

81. Cytological Bases of Regulatory Development in Echinoderms and Determination in Mollusks

Author:
A. L. Drozdov
Source:
Biology bulletin of the Russian Academy of Sciences 2021 v.48 no.5 pp. 530-535
ISSN:
1062-3590
Subject:
Bivalvia; Echinoidea; centrifugation; cortex; cytoskeleton; embryogenesis
Abstract:
... Protostomia and Deuterostomia as coelomic animals have a deterministic (mosaic) and regulatory development. The embryogenesis of Protostomia has no tendency toward regulation, which, however, is well developed in Deuterostomia. In our works, the ability to regulate the development of echinoderms, including the effect of gravitational fields on embryos, has been investigated. After centrifugation o ...
DOI:
10.1134/S1062359021040063

82. Decellularized spinach: An edible scaffold for laboratory-grown meat

Author:
Jordan D. Jones; Alex S. Rebello; Glenn R. Gaudette
Source:
Food bioscience 2021 v.41 pp. 100986
ISSN:
2212-4292
Subject:
agriculture; cattle; cellular agriculture; cost effectiveness; cytoskeleton; gelatin; glass; land; leaves; meat; muscles; myosin; satellites; spinach; viability
Abstract:
... It is projected that by the year 2050, there will be insufficient land suitable for agriculture to feed the world. Cellular agriculture has the potential to produce meat that replicates the structure of traditionally produced meat while minimizing the land needed. There is a need for an edible scaffold suitable for the growth of animal muscle. This study showed that decellularizing spinach leaves ...
DOI:
10.1016/j.fbio.2021.100986

83. Deciphering the Molecular Machinery—Influence of sE-Cadherin on Tumorigenic Traits of Prostate Cancer Cells

Author:
Igor Tsaur; Anita Thomas; Eva Juengel; Sebastian Maxeiner; Timothy Grein; Quynh Chi Le; Veronika Muschta; Jochen Rutz; Felix K. H. Chun; Roman A. Blaheta
Source:
Biology 2021 v.10 no.10 pp. -
ISSN:
2079-7737
Subject:
Western blotting; adhesion; blood serum; cadherins; cell growth; collagen; confocal microscopy; cytoskeleton; fibronectins; flow cytometry; neoplasm cells; prostatic neoplasms; therapeutics
Abstract:
... The serum level of soluble (s)E-cadherin is elevated in several malignancies, including prostate cancer (PCa). This study was designed to investigate the effects of sE-cadherin on the behavior of PCa cells in vitro, with the aim of identifying a potential therapeutic target. Growth as well as adhesive and motile behavior were evaluated in PC3, DU-145, and LNCaP cells. Flow cytometry was used to as ...
DOI:
10.3390/biology10101007

84. Delivering the pain: an overview of the type III secretion system with special consideration for aquatic pathogens

Author:
Hadis Rahmatelahi; Mansour El-Matbouli; Simon Menanteau-Ledouble
Source:
BMC veterinary research 2021 v.52 no.1 pp. 146
ISSN:
1297-9716
Subject:
Yersinia ruckeri; apoptosis; cytoskeleton; eukaryotic cells; fish; immune response; pain; pathogens; veterinary medicine; virulence
Abstract:
... Gram-negative bacteria are known to subvert eukaryotic cell physiological mechanisms using a wide array of virulence factors, among which the type three-secretion system (T3SS) is often one of the most important. The T3SS constitutes a needle-like apparatus that the bacterium uses to inject a diverse set of effector proteins directly into the cytoplasm of the host cells where they can hamper the h ...
DOI:
10.1186/s13567-021-01015-8

85. Development of fusarium wilt resistant mutants of Musa spp. cv.Rasthali (AAB, Silk subgroup) and comparative proteomic analysis along with its wild type

Author:
Gandhi Kannan; Marimuthu Somasundaram Saraswathi; Raman Thangavelu; Parasuraman Subesh Kumar; Murugesan Bathrinath; Subbaraya Uma; Suthanthiram Backiyarani; Arumugam Chandrasekar; Thumballi R. Ganapathi
Source:
Planta 2022 v.255 no.4 pp. 80
ISSN:
0032-0935
Subject:
Fusarium wilt; Musa; UTP-glucose-1-phosphate uridylyltransferase; actin; adenosinetriphosphatase; bananas; carbohydrate metabolism; cytoskeleton; energy; ethyl methanesulfonate; gene expression regulation; genes; introgression; mutagenesis; mutants; oxidoreductases; phosphopyruvate hydratase; protein subunits; protein synthesis; proteomics; respiratory burst; toxins
Abstract:
... MAIN CONCLUSION: Induced mutagenesis using embryogenic cell suspension (ECS) explants with toxin based screening is an effective tool to create non-chimeral Fusarium wilt resistant mutants in banana. Global proteomics unravel the molecular mechanism behind resistance. Race 1 of Fusarium wilt is a serious threat to Musa spp. cv.Rasthali (AAB, Silk subgroup) which is a choice variety traditionally g ...
DOI:
10.1007/s00425-022-03860-z

86. Dkk3/REIC Deficiency Impairs Spermiation, Sperm Fibrous Sheath Integrity and the Sperm Motility of Mice

Author:
Ruizhi Xue; Wenfeng Lin; Hirofumi Fujita; Jingkai Sun; Rie Kinoshita; Kazuhiko Ochiai; Junichiro Futami; Masami Watanabe; Hideyo Ohuchi; Masakiyo Sakaguchi; Zhengyan Tang; Peng Huang; Yasutomo Nasu; Hiromi Kumon
Source:
Genes 2022 v.13 no.2 pp. -
ISSN:
2073-4425
Subject:
RNA; calcium; cytoskeleton; epididymis; genes; males; mice; sequence analysis; sperm motility; spermiation; ultrastructure
Abstract:
... The role of Dickkopf-3 (Dkk3)/REIC (The Reduced Expression in Immortalized Cells), a Wnt-signaling inhibitor, in male reproductive physiology remains unknown thus far. To explore the functional details of Dkk3/REIC in the male reproductive process, we studied the Dkk3/REIC knock-out (KO) mouse model. By examining testicular sections and investigating the sperm characteristics (count, vitality and ...
DOI:
10.3390/genes13020285

87. Dysregulated HIC1 and RassF1A expression in vitro alters the cell cytoskeleton and exosomal Piwi-interacting RNA

Author:
Chiao-Yin Sun; Guo-Dung Chen; Bo-Ching He; Wei-En Fu; Chia-Huei Lee; Yu-Wei Leu; Shu-Huei Hsiao
Source:
Biochemical and biophysical research communications 2022 v.594 pp. 109-116
ISSN:
0006-291X
Subject:
DNA; biomarkers; cytoskeleton; gene expression; genomics; methylation; patients; renal cell carcinoma; research; small interfering RNA; tumor suppressor genes
Abstract:
... HIC1 and RassF1A methylation, which cause loss of gene function, are found in various cancers, including renal cell carcinoma (RCC), and could alter cell stiffness and the content of extracellular vesicles (EVs). These physiological changes may provide a tumoral survival advantage and thus could serve as cellular biomarkers for monitoring cell transformation, although direct associations between t ...
DOI:
10.1016/j.bbrc.2022.01.065

88. Dysregulation of Cell Signaling by SARS-CoV-2

Author:
Rahul K. Suryawanshi; Raghuram Koganti; Alex Agelidis; Chandrashekhar D. Patil; Deepak Shukla
Source:
Trends in microbiology 2021 v.29 no.3 pp. 224-237
ISSN:
0966-842X
Subject:
Severe acute respiratory syndrome coronavirus 2; cytoskeleton; interferons; pathogenesis; therapeutics; transcription (genetics); viruses
Abstract:
... Pathogens usurp host pathways to generate a permissive environment for their propagation. The current spread of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection presents the urgent need to understand the complex pathogen–host interplay for effective control of the virus. SARS-CoV-2 reorganizes the host cytoskeleton for efficient cell entry and controls host transcriptional pr ...
DOI:
10.1016/j.tim.2020.12.007

89. EGFR is a pivotal player of the E2/ERβ – mediated functional properties, aggressiveness, and stemness in triple‐negative breast cancer cells

Author:
Konstantina Kyriakopoulou; Elena Kefali; Zoi Piperigkou; Christoph Riethmüller; Burkhard Greve; Marco Franchi; Martin Götte; Nikos K. Karamanos
Source:
FEBS journal 2022 v.289 no.6 pp. 1552-1574
ISSN:
1742-464X
Subject:
aggression; breast neoplasms; cytoskeleton; drug therapy; epidermal growth factor receptors; estradiol; estrogen receptors; extracellular matrix; flow cytometry; fluorescence microscopy; fluorescent antibody technique; metastasis; proteoglycans; stem cells; syndecans
Abstract:
... Triple‐negative breast cancer (TNBC) is defined by aggressive behavior, limited response to chemotherapy and lower overall survival rates. The increased metastatic potential of TNBC is a combined result of extensive extracellular matrix (ECM) remodeling that leads to cytoskeleton rearrangement and activation of epithelial‐to‐mesenchymal transition (EMT). The overexpression of epidermal growth fact ...
DOI:
10.1111/febs.16240

90. Ebola Virus GP Activates Endothelial Cells via Host Cytoskeletal Signaling Factors

Author:
Benedicte Mpia Moni; Yasuteru Sakurai; Jiro Yasuda
Source:
Viruses 2022 v.14 no.1 pp. -
ISSN:
1999-4915
Subject:
Ebolavirus; apoptosis; cell adhesion; cytoskeleton; glycoproteins; hemorrhage; intercellular adhesion molecule-1; non-specific protein-tyrosine kinase; therapeutics; virus replication
Abstract:
... Ebola virus disease (EVD) is a lethal disease caused by the highly pathogenic Ebola virus (EBOV), and its major symptoms in severe cases include vascular leakage and hemorrhage. These symptoms are caused by abnormal activation and disruption of endothelial cells (ECs) whose mediators include EBOV glycoprotein (GP) without the need for viral replication. However, the detailed molecular mechanisms u ...
DOI:
10.3390/v14010142

91. Effect of ACY-1215 on cytoskeletal remodeling and histone acetylation in bovine somatic cell nuclear transfer embryos

Author:
Song Gao; Zheng Wang; Jukui Ma; Jingyi Wang; Xupeng Xing; Chenglong Shen; Zhihan Niu; Heqiang Li; Shaowei Zhang; Kaijuan Zhang; Jing Gao; Yong Zhang; Yongsheng Wang
Source:
Theriogenology 2022 v.183 pp. 98-107
ISSN:
0093-691X
Subject:
abnormal development; acetylation; animal reproduction; apoptosis; blastocyst; cattle; chromosomes; cytoskeleton; embryogenesis; epigenetics; histones; somatic cells
Abstract:
... The latest studies indicated that in addition to alterations in abnormal chromosome epigenetic modifications, the abnormal cytoskeletal changes are also an important cause for the developmental failure of somatic cell nuclear transfer (SCNT) embryos. In the present study, the effects of ACY-1215, a specific inhibitor of HDAC6, on the acetylation of α-tubulin, histone epigenetic modification, spind ...
DOI:
10.1016/j.theriogenology.2022.02.018

92. Effect of HIF1α on the TRPC6 channel of glomerular podocytes under chronic hypoxia

Author:
Guangrong Hu; Yonghan He; Yaozong Li; Xiao Hu; Sida Liu; Chang Liao; Rui Zhang; Xinyao Zhou; Hui Sun
Source:
Biochemical and biophysical research communications 2021 v.541 pp. 1-7
ISSN:
0006-291X
Subject:
actin; agonists; calcium; confocal microscopy; cytoskeleton; dose response; gene expression; humans; hypoxia; ion channels; kidney diseases; phalloidine; protein synthesis; research
Abstract:
... Chronic hypoxia plays an important role in the initiation and progression of chronic renal disease. The pathogenic role of chronic hypoxia in tubulointerstitial injury has been investigated widely, but little is known about acute hypoxia implications in glomerular damage. In this study, we investigated the effect of chronic hypoxia on transient receptor potential cation channel 6 (TRPC6) and the u ...
DOI:
10.1016/j.bbrc.2020.12.088

93. Effects of Natural Polyphenols on Oxidative Stress-Mediated Blood-Brain Barrier Dysfunction

Author:
Yeonjae Kim; A Yeon Cho; Hong Cheol Kim; Dajung Ryu; Sangmee Ahn Jo; Yi-Sook Jung
Source:
Antioxidants 2022 v.11 no.2 pp. -
ISSN:
2076-3921
Subject:
adhesion; astrocytes; blood-brain barrier; brain; brain damage; cytoskeleton; metalloproteinases; neutrophils; parenchyma (animal tissue); polyphenols; reactive oxygen species; stroke; therapeutics
Abstract:
... The blood-brain barrier (BBB), which consists mainly of brain microvascular endothelial cells and astrocytes connected by tight junctions (TJs) and adhesion molecules (AMs), maintains the homeostatic balance between brain parenchyma and extracellular fluid. Accumulating evidence shows that BBB dysfunction is a common feature of neurodegenerative diseases, including stroke, traumatic brain injury, ...
DOI:
10.3390/antiox11020197

94. Effects of calcium application on apple fruit softening during storage revealed by proteomics and phosphoproteomics

Author:
Yaxiu Xu; Jiaojiao Liu; Nannan Zang; Zepeng Yin; Aide Wang
Source:
Horticultural plant journal 2022 pp. -
ISSN:
2468-0141
Subject:
Malus; apples; calcium; cell walls; cytoskeleton; data collection; electron transport chain; ethylene production; fatty acid metabolism; fruits; glycolysis; horticultural crops; mass spectrometry; mitogen-activated protein kinase; oxidation; phosphopeptides; phosphoproteins; phosphorylation; polygalacturonase; protein degradation; proteomics; second messengers; ubiquitin
Abstract:
... Fruit ripening has been reported to be related to calcium (Ca), but the underlying mechanisms by which Ca regulates this process remain largely unknown. In order to study the changes of proteins and enriched phosphopeptides, we conducted TMT labeling, bio-material-based PTM enrichment based on mass spectrometry in Ca-treated ‘Golden Delicious’ (GD) apple fruit (Malus. × domestica). This dataset pr ...
DOI:
10.1016/j.hpj.2022.03.002

95. Entamoeba histolytica Adaption to Auranofin: A Phenotypic and Multi-Omics Characterization

Author:
Yana Shaulov; Lotem Sarid; Meirav Trebicz-Geffen; Serge Ankri
Source:
Antioxidants 2021 v.10 no.8 pp. -
ISSN:
2076-3921
Subject:
Entamoeba histolytica; cytoskeleton; homeostasis; mammals; metronidazole; multiomics; oxidation; oxidative stress; oxidoreductases; parasites; phenotype; reactive oxygen species; thioredoxins; transcriptomics; trophozoites
Abstract:
... Auranofin (AF), an antirheumatic agent, targets mammalian thioredoxin reductase (TrxR), an important enzyme controlling redox homeostasis. AF is also highly effective against a diversity of pathogenic bacteria and protozoan parasites. Here, we report on the resistance of the parasite Entamoeba histolytica to 2 µM of AF that was acquired by gradual exposure of the parasite to an increasing amount o ...
DOI:
10.3390/antiox10081240

96. Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss)

Author:
Lara Zácari Fanali; Classius De Oliveira; Joachim Sturve
Source:
Environmental science and pollution research 2021 v.28 no.38 pp. 53926-53935
ISSN:
0944-1344
Subject:
Oncorhynchus mykiss; biotransformation; blood; cytoskeleton; cytotoxicity; fish; genotoxicity; genotoxicity tests; liver; melanin; mutagens; pollution; research; xenobiotics
Abstract:
... Fish have defense systems that are capable of repairing damages caused by xenobiotics like benzo[a]pyrene (BaP), so the aims of this study were to identify BaP toxicity in melanomacrophages (MMs) cytoskeleton, evaluate the melanin area in MMs, and analyze genotoxicity. Rainbow trout juveniles (n = 24) were split in 48h and 7d treatments that received 2 mg/kg of BaP. After the experiment, blood sam ...
DOI:
10.1007/s11356-021-14583-1

97. Epithelial-mesenchymal transition process during embryo implantation

Author:
Farnaz Oghbaei; Reza Zarezadeh; Davoud Jafari-Gharabaghlou; Minoo Ranjbar; Mohammad Nouri; Amir Fattahi; Kazuhiko Imakawa
Source:
Cell and tissue research 2022 v.388 no.1 pp. 1-17
ISSN:
0302-766X
Subject:
aquaporins; conceptus; cytoskeleton; embryo implantation; endometrium; epithelium; microRNA; neoplasm cells; research; trophoblast
Abstract:
... The epithelial to mesenchymal transition (EMT) in endometrial epithelial and trophectoderm cells is essential for the progression of embryo implantation and its impairment could cause implantation failure. Therefore, EMT should be tightly regulated in both embryonic and endometrial cells during implantation. Studies reported the involvement of numerous factors in EMT regulation, including hormones ...
DOI:
10.1007/s00441-021-03574-w

98. Ethanol affects fibroblast behavior differentially at low and high doses: A comprehensive, dose-response evaluation

Author:
Neelakshi Kar; Deepak Gupta; Jayesh Bellare
Source:
Toxicology reports 2021 v.8 pp. 1054-1066
ISSN:
2214-7500
Subject:
cell proliferation; cell viability; cytoskeleton; ethanol; fibroblasts; hormesis; mitochondria; oxidative stress; toxicity; toxicology
Abstract:
... This study aims to develop a comprehensive understanding of effects of low and high doses of ethanol on cellular biochemistry and morphology. Here, fibroblast cells are exposed to ethanol of varied concentrations [0.005−10 % (v/v)] to investigate cellular activity, cytoskeletal organization, cellular stiffness, mitochondrial structure, and real-time behavior. Our results indicate a sharp differenc ...
DOI:
10.1016/j.toxrep.2021.05.007

99. Evidence of Transcriptional Shutoff by Pathogenic Viral Haemorrhagic Septicaemia Virus in Rainbow Trout

Author:
Irene Cano; Eduarda M. Santos; Karen Moore; Audrey Farbos; Ronny van Aerle
Source:
Viruses 2021 v.13 no.6 pp. -
ISSN:
1999-4915
Subject:
Oncorhynchus mykiss; Viral hemorrhagic septicemia virus; cell lines; cytoskeleton; gene expression regulation; genes; immune response; lipid metabolism; pathogenicity; peroxisome proliferator-activated receptors; salmonellosis; transcription (genetics); transcriptome; virus replication
Abstract:
... The basis of pathogenicity of viral haemorrhagic septicaemia virus (VHSV) was analysed in the transcriptome of a rainbow trout cell line inoculated with pathogenic and non-pathogenic VHSV isolates. Although both VHSV isolates showed similar viral replication patterns, the number of differentially expressed genes was 42-fold higher in cells inoculated with the non-pathogenic VHSV at 3 h post inocul ...
DOI:
10.3390/v13061129

100. Exacerbation of copper pollution toxicity from ocean acidification: A comparative analysis of two bivalve species with distinct sensitivities

Author:
Ruiwen Cao; Yiling Zhang; Yuhao Ju; Wei Wang; Chenxiang Xi; Wenlin Liu; Kai Liu
Source:
Environmental pollution 2022 v.293 pp. 118525
ISSN:
0269-7491
Subject:
Bivalvia; apoptosis; bioaccumulation; biomarkers; carbon dioxide; cytoskeleton; energy metabolism; estuaries; health status; ocean acidification; pH; pollution; protein metabolism; proteomics; seawater; signal transduction; toxicity; viability
Abstract:
... In estuarine ecosystems, bivalves experience large pH fluctuations caused by the anthropogenic elevation of atmospheric CO₂ and Cu pollution. This study investigates whether Cu toxicity increases indiscriminately in two bivalve species from different estuarine habitats as a result of elevated Cu bioaccumulation in acidified seawater. This was carried out by evaluating the effects of Cu exposure on ...
DOI:
10.1016/j.envpol.2021.118525