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- Pirolla, Renan Augusto Siqueira, et al. Show all 6 Authors
- Journal of molecular biology 2019 v.431 no.4 pp. 732-747
- Bacteroidetes; Bifidobacterium; active sites; bees; beta-mannosidase; carbohydrates; depolymerization; digestive system; ecosystems; hosts; humans; intestinal microorganisms; moieties; nutrition; probiotics; rabbits; swine; symbionts; tryptophan
- ... Bifidobacteria represent one of the first colonizers of human gut microbiota, providing to this ecosystem better health and nutrition. To maintain a mutualistic relationship, they have enzymes to degrade and use complex carbohydrates non-digestible by their hosts. To succeed in the densely populated gut environment, they evolved molecular strategies that remain poorly understood. Herein, we report ...
- Pirolla, Renan Augusto Siqueira, et al. Show all 15 Authors
- Biotechnology for biofuels 2018 v.11 no.1 pp. 223
- alpha-N-arabinofuranosidase; arabinoxylan; biomass; cell walls; corn stover; crystal structure; depolymerization; fungi; grasses; hydrolysis; mass spectrometry; reducing sugars; saccharification; sugarcane; sugarcane bagasse; xylan
- ... BACKGROUND: Arabinoxylan is an abundant polysaccharide in industrially relevant biomasses such as sugarcane, corn stover and grasses. However, the arabinofuranosyl di-substitutions that decorate the xylan backbone are recalcitrant to most known arabinofuranosidases (Abfs). RESULTS: In this work, we identified a novel GH51 Abf (XacAbf51) that forms trimers in solution and can cope efficiently with ...
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