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- Sakita, Masashi, et al. Show all 25 Authors
- Biologicals 2011 v.39 no.3 pp. 171-180
- acid hydrolysis; anion exchange chromatography; biopharmaceuticals; derivatization; digestion; fluorometry; glycoproteins; glycosylation; high performance liquid chromatography; models; monosaccharides; pH; sialic acids; sialidase
- ... The various monosaccharide composition analysis methods were evaluated as monosaccharide test for glycoprotein-based pharmaceuticals. Neutral and amino sugars were released by hydrolysis with 4–7N trifluoroacetic acid. The monosaccharides were N-acetylated if necessary, and analyzed by high-performance liquid chromatography (HPLC) with fluorometric or UV detection after derivatization with 2-amino ...